Spectral gap for products of $PSL(2,bbR)$

V GS - Gate-to-Source Voltage - VRDS(on)-On-StateResistance-mΩ01234567891051015202530G006Q g - Gate Charge - nCVGS-Gate-to-SourceVoltage-V01234567G003CSD17307Q5A SLPS252A–FEBRUARY2010–REVISED JULY201030V,N-Channel NexFET™Power MOSFETsCheck for Samples:CSD17307Q5AFEATURESPRODUCT SUMMARY•Optimized for5V Gate DriveV DS Drain to Source Voltage30V •Ultralow Q g and Q gdQ g Gate Charge Total(4.5V)4nC •Low Thermal ResistanceQ gd Gate Charge Gate to Drain1nC •Avalanche Rated VGS=3V12.8mΩR DS(on)Drain to Source On Resistance V GS=4.5V9.7mΩ•Pb Free Terminal PlatingV GS=8V8.4mΩ•RoHS CompliantV GS(th)Threshold Voltage 1.3V •Halogen Free•SON5-mm×6-mm Plastic PackageORDERING INFORMATIONDevice Package Media Qty Ship APPLICATIONSSON5-mm×6-mm13-Inch Tape andCSD17307Q5A2500•Notebook Point of Load Plastic Package Reel Reel •Point-of-Load Synchronous Buck inNetworking,Telecom and Computing Systems ABSOLUTE MAXIMUM RATINGST A=25°C unless otherwise stated VALUE UNIT DESCRIPTIONV DS Drain to Source Voltage30VV GS Gate to Source Voltage+10/–8V The NexFET™power MOSFET has been designedContinuous Drain Current,T C=25°C73Ato minimize losses in power conversion applications,I Dand optimized for5V gate drive applications.Continuous Drain Current(1)14AI DM Pulsed Drain Current,T A=25°C(2)92ATop View PDPower Dissipation(1)3WT J,Operating Junction and Storage–55to150°CT STG Temperature RangeAvalanche Energy,Single PulseE AS54mJI D=33A,L=0.1mH,R G=25Ω(1)Typical R q JA=41°C/W on a1-inch2(6.45-cm2),2-oz.(0.071-mm thick)Cu pad on a0.06-inch(1.52-mm)thickFR4PCB.(2)Pulse duration≤300m s,duty cycle≤2%GATE CHARGEPlease be aware that an important notice concerning availability,standard warranty,and use in critical applications of TexasInstruments semiconductor products and disclaimers thereto appears at the end of this data sheet.NexFET is a trademark of Texas Instruments.PRODUCTION DATA information is current as of publication date.Copyright©2010,Texas Instruments Incorporated Products conform to specifications per the terms of the TexasInstruments standard warranty.Production processing does notnecessarily include testing of all parameters.CSD17307Q5ASLPS252A–FEBRUARY2010–REVISED These devices have limited built-in ESD protection.The leads should be shorted together or the device placed in conductive foam during storage or handling to prevent electrostatic damage to the MOS gates.ELECTRICAL CHARACTERISTICS(T A=25°C unless otherwise stated)PARAMETER TEST CONDITIONS MIN TYP MAX UNIT Static CharacteristicsBV DSS Drain to Source Voltage V GS=0V,I D=250m A30VI DSS Drain to Source Leakage Current V GS=0V,V DS=24V1m AI GSS Gate to Source Leakage Current V DS=0V,V GS=+10/–8V100nAV GS(th)Gate to Source Threshold Voltage V DS=V GS,I D=250m A0.9 1.3 1.8VV GS=3V,I D=11A12.817.3mΩR DS(on)Drain to Source On Resistance V GS=4.5V,I D=11A9.712.1mΩV GS=8V,I D=11A8.410.5mΩg fs Transconductance V DS=15V,I D=11A66S Dynamic CharacteristicsC iss Input Capacitance535700pFC oss Output Capacitance V GS=0V,V DS=15V,f=1MHz290375pFC rss Reverse Transfer Capacitance2836pFR G Series Gate Resistance0.9 1.8ΩQ g Gate Charge Total(4.5V)4 5.2nCQ gd Gate Charge Gate to Drain1nCV DS=15V,I D=11AQ gs Gate Charge Gate to Source 1.3nCQ g(th)Gate Charge at Vth0.65nCQ oss Output Charge V DS=13V,V GS=0V7.3nCt d(on)Turn On Delay Time 4.6nst r Rise Time 6.7nsV DS=15V,V GS=4.5V,I DS=11A,R G=2Ωt d(off)Turn Off Delay Time9.3nst f Fall Time 2.6ns Diode CharacteristicsV SD Diode Forward Voltage I SD=11A,V GS=0V0.851VQ rr Reverse Recovery Charge13nCV DD=13V,I F=11A,di/dt=300A/m st rr Reverse Recovery Time16ns THERMAL CHARACTERISTICS(T A=25°C unless otherwise stated)PARAMETER MIN TYP MAX UNITR q JC Thermal Resistance Junction to Case(1) 1.9°C/WR q JA Thermal Resistance Junction to Ambient(1)(2)52°C/W (1)R q JC is determined with the device mounted on a1-inch2(6.45-cm2),2-oz.(0.071-mm thick)Cu pad on a1.5-inch×1.5-inch(3.81-cm×3.81-cm),0.06-inch(1.52-mm)thick FR4PCB.R q JC is specified by design,whereas R q JA is determined by the user’s board design. (2)Device mounted on FR4material with1-inch2(6.45-cm2),2-oz.(0.071-mm thick)Cu.2Submit Documentation Feedback Copyright©2010,Texas Instruments IncorporatedProduct Folder Link(s):CSD17307Q5AGATE SourceDRAINN-Chan 5x6 QFN TTA MIN Rev3M0137-02GATESourceDRAINN-Chan 5x6 QFN TTA MAX Rev3M0137-01t - Pulse Duration - sp 0.00010.010.11101001k0.0010.010.1110G012Z - N o r m a l i z e d T h e r m a l I m p e d a n c eq J A0.001CSD17307Q5ASLPS252A –FEBRUARY 2010–REVISED JULY 2010Max R q JA =52°C/W Max R q JA =121°C/W when mounted on when mounted on a 1inch 2(6.45cm 2)of minimum pad area of 2-oz.(0.071-mm thick)2-oz.(0.071-mm thick)Cu.Cu.TYPICAL MOSFET CHARACTERISTICS(T A =25°C unless otherwise stated)Figure 1.Transient Thermal ImpedanceCopyright ©2010,Texas Instruments Incorporated Submit Documentation Feedback3Product Folder Link(s):CSD17307Q5AV DS - Drain-to-Source Voltage - VI D S - D r a i n -t o -S o u r c e C u r r e n t - A051015202530G001V GS - Gate-to-Source Voltage - VG002Q g - Gate Charge - nCV G S - G a t e -t o -S o u r c e V o l t a g e - V1234567012345678G003C - C a p a c i t a n c e - n FT C - Case Temperature - °C-75-25257512517500.20.40.60.811.21.41.61.8G005V GS - Gate-to-Source Voltage - VR D S (o n ) - O n -S t a t e R e s i s t a n c e - m Ω123456789100510********G006CSD17307Q5ASLPS252A –FEBRUARY 2010–REVISED JULY 2010TYPICAL MOSFET CHARACTERISTICS (continued)(T A =25°C unless otherwise stated)Figure 2.Saturation CharacteristicsFigure 3.Transfer Characteristics Figure 4.Gate Charge Figure 6.Threshold Voltage vs.Temperature Figure 7.On-State Resistance vs.Gate-to-Source Voltage4Submit Documentation FeedbackCopyright ©2010,Texas Instruments IncorporatedProduct Folder Link(s):CSD17307Q5AT C - Case Temperature - °CN o r m a l i z e d O n -S t a t e R e s i s t a n c e-75-2525751251750.20.40.60.81.21.41.61G007V SD - Source-to-Drain Voltage - VI S D - S o u r c e -t o -D r a i n C u r r e n t - A00.20.40.60.81 1.20.00010.0010.010.1110100G008V DS - Drain-to-Source Voltage - V0.010.11101000.010.11101001kG009I D S - D r a i n -t o -S o u r c e C u r r e n t - At (AV) - Time in Avalanche - ms0.010.1110110100G010I (A V ) - P e a k A v a l a n c h e C u r r e n t - AT C - Case Temperature - °CI D S - D r a i n -t o -S o u r c e C u r r e n t - A-50-252550751001251501750102030405060708090100G011CSD17307Q5ASLPS252A –FEBRUARY 2010–REVISED JULY 2010TYPICAL MOSFET CHARACTERISTICS (continued)(T A =25°C unless otherwise stated)Figure 8.Normalized On-State Resistance vs.TemperatureFigure 9.Typical Diode Forward VoltageFigure 10.Maximum Safe Operating Area Figure 11.Single Pulse Unclamped Inductive SwitchingFigure 12.Maximum Drain Current vs.TemperatureCopyright ©2010,Texas Instruments Incorporated Submit Documentation Feedback5Product Folder Link(s):CSD17307Q5ATop ViewFront ViewSide ViewM0135-01bBottom ViewCSD17307Q5ASLPS252A–FEBRUARY2010–REVISED MECHANICAL DATAQ5A Package DimensionsMILLIMETERSDIMMIN NOM MAXA0.90 1.00 1.10b0.330.410.51c0.200.250.34D1 4.80 4.90 5.00D2 3.61 3.81 4.02E 5.90 6.00 6.10E1 5.70 5.75 5.80E2 3.38 3.58 3.78e 1.17 1.27 1.37H0.410.560.71K 1.10L0.510.610.71L10.060.130.20q0°12°6Submit Documentation Feedback Copyright©2010,Texas Instruments IncorporatedProduct Folder Link(s):CSD17307Q5AM0139-010.10K00.30 ±0.05B0 = 5.30 ±0.10K0 = 1.40 ±0.10M0138-01CSD17307Q5ASLPS252A –FEBRUARY 2010–REVISED JULY 2010MILLIMETERS INCHESDIM Recommended PCB PatternMIN MAX MINMAX F1 6.205 6.3050.2440.248F2 4.46 4.560.1760.18F34.46 4.560.1760.18F40.650.70.0260.028F50.620.670.0240.026F60.630.680.0250.027F70.70.80.0280.031F80.650.70.0260.028F90.620.670.0240.026F10 4.950.1930.197F114.464.560.1760.18For recommended circuit layout for PCB designs,see application note SLPA005–Reducing Ringing Through PCB Layout Techniques .Q5A Tape and Reel InformationNotes:1.10-sprocket hole-pitch cumulative tolerance ±0.22.Camber not to exceed 1mm in 100mm,noncumulative over 250mm3.Material:black static-dissipative polystyrene4.All dimensions are in mm (unless otherwise specified)5.A0and B0measured on a plane 0.3mm above the bottom of the pocketCopyright ©2010,Texas Instruments Incorporated Submit Documentation Feedback7Product Folder Link(s):CSD17307Q5ACSD17307Q5ASLPS252A–FEBRUARY2010–REVISED REVISION HISTORYChanges from Original(February2010)to Revision A Page •Updated the Q5A Package Dimensions table.DIM c MAX was0.30,DIM D2MAX was3.96,DIM e MIN was blank MAX was blank,DIM H NOM was0.51MAX was0.61 (6)•Deleted Note6from the Q5A Tape and Reel Information-"MSL1260°C(IR and convection)PbF reflow compatible" (7)•Deleted the Package Marking Information section (7)8Submit Documentation Feedback Copyright©2010,Texas Instruments IncorporatedProduct Folder Link(s):CSD17307Q5APACKAGE OPTION ADDENDUM6-Feb-2020Addendum-Page 1PACKAGING INFORMATION(1)The marketing status values are defined as follows:ACTIVE: Product device recommended for new designs.LIFEBUY: TI has announced that the device will be discontinued, and a lifetime-buy period is in effect.NRND: Not recommended for new designs. Device is in production to support existing customers, but TI does not recommend using this part in a new design.PREVIEW: Device has been announced but is not in production. Samples may or may not be available.OBSOLETE: TI has discontinued the production of the device.(2)RoHS: TI defines "RoHS" to mean semiconductor products that are compliant with the current EU RoHS requirements for all 10 RoHS substances, including the requirement that RoHS substance do not exceed 0.1% by weight in homogeneous materials. Where designed to be soldered at high temperatures, "RoHS" products are suitable for use in specified lead-free processes. TI may reference these types of products as "Pb-Free".RoHS Exempt: TI defines "RoHS Exempt" to mean products that contain lead but are compliant with EU RoHS pursuant to a specific EU RoHS exemption.Green: TI defines "Green" to mean the content of Chlorine (Cl) and Bromine (Br) based flame retardants meet JS709B low halogen requirements of <=1000ppm threshold. 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MagMAX ™ FFPE DNA/RNA Ultra KitAutomated or manual isolation of total nucleic acid (TNA) from FFPE samples using AutoLys tubesCatalog Number A31881Pub. No. MAN0017538 Rev.A.0WARNING! Read the Safety Data Sheets (SDSs) and follow thehandling instructions. Wear appropriate protective eyewear,clothing, and gloves. Safety Data Sheets (SDSs) are available from /support .Product descriptionThe Applied Biosystems ™ MagMAX ™ FFPE DNA/RNA Ultra Kit is designed to isolate both DNA and RNA from the same section offormaldehyde- or paraformaldehyde-fixed, paraffin-embedded (FFPE)tissues. The kit also allows for flexibility to isolate DNA only, RNA only or total nucleic acid (TNA). The kit uses MagMAX ™ magnetic-bead technology, ensuring reproducible recovery of high-quality nucleic acid through manual or automated processing. The isolated nucleic acid is appropriate for use with a broad range of downstream assays, such as quantitative real-time RT-PCR and next-generation sequencing.In addition to the use of traditional solvents, the kit is compatible with Autolys M tubes that enable a faster and more convenient means of deparaffinizing FFPE samples by eliminating the need for organic solvents such as xylene or CitriSolv and ethanol. Samples are put into the tubes for protease digestion, tubes are lifted with the Auto-pliers or Auto-Lifter and then samples are spun down. The wax and debris are contained in the upper chamber while the lysate is passed through.Afterwards, the clarified lysate can be directly purified with the MagMAX ™ FFPE DNA/RNA Ultra Kit.For guides without using AutoLys M tubes for sequential DNA and RNA isolation, or DNA isolation, or RNA isolation only, seeMagMAX ™ FFPE DNA/RNA Ultra Kit User Guide (sequential DNA/RNA isolation) (Pub. No. MAN0015877), or MagMAX ™ FFPE DNA/RNA Ultra Kit User Guide (DNA isolation only) (Pub. No. MAN0015905), or MagMAX ™ FFPE DNA/RNA Ultra Kit User Guide (RNA isolation only)(Pub. No. MAN0015906), respectively.This guide describes isolation of TNA from FFPE tissue blocks or FFPE slides using AutoLys M tubes. Three optimized methods for sections or curls both up to 40 µm using AutoLys M tubes are included:•Manual sample processing.•KingFisher ™ Flex Magnetic Particle Processor with 96 Deep-Well Head (DW96; 96-well deep well setting).•KingFisher ™ Duo Prime Magnetic Particle Processor (12-well deep well setting).For sequential DNA and RNA isolation, or DNA isolation, or RNA isolation only, see MagMAX ™ FFPE DNA/RNA Ultra Kit User Guide (sequential DNA/RNA isolation) (Pub. No. MAN0017541), MagMAX ™FFPE DNA/RNA Ultra Kit User Guide (DNA isolation only)(Pub. No. MAN0017539), or MagMAX ™ FFPE DNA/RNA Ultra Kit User Guide (RNA isolation only) (Pub. No. MAN0017540), respectively.Contents and storageReagents provided in the kit are sufficient for 48 TNA isolations from sections up to 40 µm with the AutoLys workflow.Table 1 MagMAX ™ FFPE DNA/RNA Ultra Kit (Cat. No. A31881)Additional reagents are available separately; Protease Digestion Buffer, Binding Solution, and DNA Wash Buffer are also available as Cat. No. A32796. [2]Shipped at room temperature. [3]Not used in TNA workflow[4]Final volume; see “Isolate TNA“ on page 4.Required materials not suppliedUnless otherwise indicated, all materials are available through . MLS: Fisher Scientific ( ) or other major laboratory supplier.Table 2 Materials required for nucleic acid isolation (all methods)Table 3 Additional materials required for manual isolationTable 4 Additional materials required for automated isolationIf needed, download the KingFisher ™ Duo Prime or Flex programThe programs required for this protocol are not pre-installed on theKingFisher ™Duo Prime Magnetic Particle Processor or on theKingFisher ™Flex Magnetic Particle Processor 96DW.1.On the MagMAX ™ FFPE DNA/RNA Ultra Kit product web page,scroll down to the Product Literature section.2.Right-click on the appropriate program file(s) for your samplesize to download the program to your computer:3.4.Refer to the manufacturer's documentation for instructions forinstalling the program on the instrument.Procedural guidelines•Perform all steps at room temperature (20–25°C) unless otherwise noted.•When mixing samples by pipetting up and down, avoid creating bubbles.•When working with RNA:–Wear clean gloves and a clean lab coat.–Change gloves whenever you suspect that they are contaminated.–Open and close all sample tubes carefully. Avoid splashing or spraying samples.–Use a positive-displacement pipettor and RNase-free pipette tips.–Clean lab benches and equipment periodically with an RNase decontamination solution, such as RNase Zap ™ Solution (Cat.No. AM9780).•Incubation at 60°C can be extended overnight to increase DNA yields, followed by incubation at 90°C for 1 hour.•Volumes for reagent mixes are given per sample. We recommend that you prepare master mixes for larger sample numbers. To calculate volumes for master mixes, refer to the per-well volume and add 5–10% overage.Before you beginBefore first use of the kit•Prepare the Wash Solutions from the concentrates:–Add 46 mL of isopropanol to RNA Wash Buffer Concentrate ,mix, and store at room temperature.–Add 168 mL of ethanol to Wash Solution 2 Concentrate , mix,and store at room temperature.Before each use of the kit•Equilibrate the Nucleic Acid Binding Beads to room temperature.•Pre-heat the incubators or ovens to 60°C and 90°C.•Prepare the following solutions according to the following tables.Table 5 Protease solutionTable 6 TNA Binding BufferPrepare the FFPE samples•For curls from FFPE tissue blocks: proceed to “Prepare the curls from FFPE tissue blocks“ on page 3.•For FFPE slide-mounted sections: proceed to “Prepare samples from FFPE slides“ on page 3.Prepare the curls from FFPE tissue blocksa.Cut sections from FFPE tissue blocks using a microtome.Note: For miRNA extraction, we recommend using sections of 10 µm or thicker.b.Collect each section in an AutoLys M tube.1Section FFPE tissue blocks a.Add 235 µL of the Protease Solution (see Table 5).Note: If working with curls, they might stick straight up so make sure to submerge samples in theProtease Solution with a tip or a 1 mL syringe plunger or do a quick spin down at 3000 rpm for 1 minute prior to the addition of buffer to collapse the curl. Time may be extended.b.Incubate at 60°C for 1 hour or longer.Note: Use the AutoLys racks and place in an incubator or oven.c.Incubate at 90°C for 1 hour.Note: For automated isolation, set up the processing plates during the incubation.·For isolation using KingFisher ™ Duo Prime Magnetic Particle Processor, proceed to “Set up the processing plate“ on page 4.·For isolation using KingFisher ™ Flex Magnetic Particle Processor 96DW, proceed to “Set up the TNA processing plates“ on page 5.2Digest with Protease a.Allow samples to cool down for 3–5 minutes before proceeding to lift the tubes.e the Auto-plier for individual tube lifting or the Auto-lifter for multiple tube lifting of up to 24 tubes.c.Lock the tubes in position by hand or use the locking lid.d.Centrifuge at 2000 × g for 10 minutes in a benchtop centrifuge with plate adapters.e.Unlock the tubes by hand or remove the locking lid.e the Auto-plier or Auto-lifer to lift the inner tube for sample access.g.Proceed to purification. See “Isolate TNA“ on page 43Lift the tubesPrepare samples from FFPE slidesa.Pipet 2–4 µL of Protease Digestion Buffer depending on the tissue size evenly across the FFPE tissuesection on the slide to pre-wet the section.Note: You can adjust the volume of Protease Digestion Buffer if the tissue is smaller or larger.b.Scrape the tissue sections in a single direction with a clean razor blade or scalpel, then collect the tissueon the slide into a cohesive mass.c.Transfer the tissue mass into an AutoLys M tube with the scalpel or a pipette tip.d.Add 235 µL of the Protease Solution (see Table 5).Note: Be sure to submerge samples in the Protease Solution with a tip or a 1 mL syringe plunger e.Incubate at 60°C for 1 hour or longer.Note: Use the AutoLys racks and place in an incubator or oven.f.Incubate at 90°C for 1 hour.Note: For automated isolation, set up the processing plates during the incubation.·For isolation using KingFisher ™ Duo Prime Magnetic Particle Processor, proceed to “Set up the processing plate“ on page 4.·For isolation using KingFisher ™ Flex Magnetic Particle Processor 96DW, proceed to “Set up the TNA processing plates“ on page 5.4Scrape the samples and digest with Proteasea.Allow samples to cool down for 3–5 minutes before proceeding to lift the tubes.e the Auto-plier for individual tube lifting or the Auto-lifter for multiple tube lifting of up to 24 tubes.c.Lock the tubes in position by hand or use the locking lid.d.Centrifuge at 2000 × g for 10 minutes in a benchtop centrifuge with plate adapters.e.Unlock the tubes by hand or remove the locking lid.e the Auto-plier or Auto-lifer to lift the inner tube for sample access.g.Proceed to purification. See “Isolate TNA“ on page 45Lift the tubesIsolate TNA•To isolate TNA manually, proceed to “Isolate TNA manually“ on page 4.•To isolate TNA using the KingFisher ™Duo Prime Magnetic Particle Processor, proceed to “Isolate TNA using KingFisher ™ Duo Prime Magnetic Particle Processor“ on page 4.•To isolate TNA using the KingFisher ™Flex Magnetic Particle Processor 96DW, proceed to “Isolate TNA using KingFisher ™ Flex Magnetic Particle Processor 96DW“ on page 5.Isolate TNA manuallyUse microcentrifuge tubes to perform manual TNA isolations.a.After the Protease digestion is complete, add 20 µL of Nucleic Acid Binding Beads to the samples.b.Add 900 µL of TNA Binding Buffer (see Table 6) to the sample.c.Shake for 5 minutes at speed 10 or 1150 rpm.d.Place the sample on the magnetic stand for 2 minutes or until the solution clears and the beads are pelleted against the magnet.e.Carefully discard the supernatant with a pipette.1Bind the TNA to beadsa.Wash the beads with 500 µL of RNA Wash Buffer.b.Shake for 1 minute at speed 10 or 1150 rpm until the mixture is thoroughly chocolate brown in color.c.Place the sample on the magnetic stand for 2 minutes or until the solution clears and the beads arepelleted against the magnet.d.Carefully discard the supernatant with a pipette.e.Repeat steps a-d.f.Wash the beads with 500 µL of Wash Solution 2.g.Shake for 1 minute at speed 10 or 1150 rpm until the mixture is thoroughly chocolate brown in color.h.Place the sample on the magnetic stand for 2 minutes or until the solution clears and the beads arepelleted against the magnet.i.Carefully discard the supernatant with a pipette.j.Repeat steps f-i.k.Shake for 1–2 minutes at speed 10 or 1150 rpm to dry the beads.Do not over-dry the beads. Over-dried beads results in low TNA recovery yields.2Wash TNA on the beadsa.Add 50 µL of Elution Solution to the beads.b.Shake for 5 minutes at speed 10 or 1150 rpm and at 55°C until the mixture is thoroughly chocolatebrown in color.c.Place the sample on the magnetic stand for 2 minutes or until the solution clears and the beads arepelleted against the magnet.The supernatant contains the purified TNAThe purified TNA is ready for immediate use. Store at –20°C or –80°C for long-term storage.3Elute the TNAIsolate TNA using KingFisher ™ Duo Prime Magnetic Particle ProcessorDuring the protease incubation, add processing reagents to the wells of a MagMAX ™ Express-96 Deep WellPlate as indicated in the following table.Table 7 TNA plate setupRow on the MagMAX ™ Express-96 Deep Well Plate.4Set up the processing plate a.Ensure that the instrument is set up for processing with the deep well 96–well plates and select theappropriate program A31881_DUO_large_vol_TNA on the instrument.b.At the end of the protease incubation, add 200 µL of sample to each well in Row G of the TNA plate.c.Add 20 µL of Nucleic Acid Binding Beads to each sample well in Row G.d.Start the run and load the prepared processing plates when prompted by the instrument.5Bind, wash, rebind, and elute the TNA5Bind, wash, rebind,and elute the TNA(continued)e.At the end of the run, remove the Elution Plate from the instrument and transfer the eluted TNA(Row A of TNA plate) to a new plate and seal immediately with a new MicroAmp ™ Clear Adhesive Film.IMPORTANT! Do not allow the purified samples to sit uncovered at room temperature for more than 10minutes, to prevent evaporation and contamination.The purified TNA is ready for immediate use. Store at –20°C or –80°C for long-term storage.Isolate TNA using KingFisher ™ Flex Magnetic Particle Processor 96DWDuring the protease incubation, add processing reagents to the wells of MagMAX ™ Express-96 Plates asindicated in the following table.Table 8 TNA plates setupPosition on the instrument6Set up the TNA processing platesa.Ensure that the instrument is set up for processing with the deep well magnetic head and select theA31881_FLEX_large_vol_TNA program on the instrument.b.At the end of the protease incubation, add 200 µL of sample to each well in Plate 1.c.Add 20 µL of Nucleic Acid Binding Beads to each sample well in Plate 1.d.Start the run and load the prepared processing plates in their positions when prompted by theinstrument (see “Set up the TNA processing plates“ on page 5).e.At the end of the run, remove the Elution Plate from the instrument and seal immediately with a newMicroAmp ™ Clear Adhesive Film.IMPORTANT! Do not allow the purified samples to sit uncovered at room temperature for more than 10minutes, to prevent evaporation and contamination.The purified TNA is ready for immediate use. Store at –20°C or –80°C for long-term storage. .7Bind, wash, rebind, and elute the TNALimited product warrantyLife Technologies Corporation and/or its affiliate(s) warrant their products as set forth in the Life Technologies' General Terms and Conditions of Sale found on Life Technologies' website at /us/en/home/global/terms-and-conditions.html . If you have any questions,please contact Life Technologies at /support .Manufacturer: Life Technologies Corporation | 2130 Woodward Street | Austin, TX 78744The information in this guide is subject to change without notice.DISCLAIMER : TO THE EXTENT ALLOWED BY LAW, LIFE TECHNOLOGIES AND/OR ITS AFFILIATE(S) WILL NOT BE LIABLE FOR SPECIAL, INCIDENTAL, INDIRECT, PUNITIVE,MULTIPLE, OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR ARISING FROM THIS DOCUMENT, INCLUDING YOUR USE OF IT.Important Licensing Information : This product may be covered by one or more Limited Use Label Licenses. By use of this product, you accept the terms and conditions of all applicable Limited Use Label Licenses.©2018 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified./support | /askaquestion 。

普通照明用LED产品光生物安全研究吴启保;李金玉;王勇;唐飞;周钢【摘要】分析了白光LED制造技术及照明产品光生物安全性，认为可用蓝光光化学危害来评价其光生物安全等级，并介绍了蓝光危害评估的测量方法。

对常用八款产品按照标准进行测评，结果表明目前主流产品是无危害的。

提出了提升LED照明产品光生物安全性的建议。

%The photobiological safety of white LED and lighting products is analyzed. The method of measuring blue light hazard of LED products is introduced. Measurement and risk classification for eight types of LED general lighting products is carried out in accordance with standards. Testing results shows that the current status of the main LED lighting products is good. Some suggestions for how to improve photobiological safety are presented.【期刊名称】《深圳信息职业技术学院学报》【年(卷),期】2015(000)001【总页数】3页(P51-53)【关键词】LED;光生物安全;蓝光危害;测量方法;等级分类【作者】吴启保;李金玉;王勇;唐飞;周钢【作者单位】深圳信息职业技术学院机电工程学院，广东深圳518172;广东轻工职业技术学院环境工程系，广东广州510300;深圳信息职业技术学院机电工程学院，广东深圳518172;深圳信息职业技术学院机电工程学院，广东深圳518172;广州赛西光电标准检测研究院有限公司，广东广州510163【正文语种】中文【中图分类】TM923光生物安全问题包括紫外光、可见光和红外光辐射对人体眼睛和皮肤等器官危害。

bcl-2 PCR Primers Set Product No. B9179 Store at –20 ºCProduct DescriptionIn recent years, several genes have been linked to apoptosis. The bcl-2 family of genes regulates PCD either positively or negatively. Bcl-2 and members of its family have been found to block apoptotic cell death. Bcl-2 protein heterodimerizes with Bax (Bcl-2 Associated X protein), which is a potent mediator of programmed cell death. The Bcl-2/Bax ratio appears to determine whether some cells live or die.1-4The bcl-2 PCR Pr imers Set contains both sense and antisense primers for the amplification of the bcl-2αgene. It is designed for PCR† detection of human, rat and mouse cDNA levels (representing mRNA expression) of the bcl-2α apoptotic gene. No ampli-fication of the genomic DNA has been observed.The size of the amplified product resulting from the use of the bcl-2 PCR Primers Set is 127 bp.Component• bcl-2 PCR Primers Set, Product No. B9179 1 vial Equipment and Reagents Required but Not Provided (Sigma product numbers have been given where appropriate)• Thermal cycler• Taq DNA polymerase, Product No. D4545 or equivalent• Deoxynucleotide mix, 10 mM, Product No. D7295 or equivalent• Agarose• Ethidium bromide, 500 µg/ml, Product No. E1385 • PCR 100 bp low ladder, Product No. P1473• Gel loading solutions, Product No. G2526 or G7654• PCR grade water, Product No. W1754• Mineral oil, Product No. M8662• PCR microtubes, Product No. Z37,487-3 or Z37,496-2StorageStore the vial at −20 °C.Preparation InstructionsThe bcl-2 PCR Primers Set contains 1 nmoles of each primer (sense and antisense). Centrifuge the tube briefly in order to collect the tube contents. For the following procedure, resuspend the primers set in100 µl deionized water to a final concentration of10 pmole/µl. Mix until the solution is homogenous. Once suspended, store the solution at –20 °C. To avoid repeated freeze-thaw cycles, aliquot the primer solution for long-term storage.ProcedureNote: Use aseptic techniques and use aerosol barrier tips while performing PCR experiments.1. Thaw the bcl-2 PCR Primers Set on ice, beingsure that the solution is homogenous.2. Add the following reagents to a PCRmicrocentrifuge tube in the following order:Amount for50 µl singlePCRreactionFinalconcentrationin the PCRreaction Water To 50 µl ----10X PCR Buffer 5 µl 1X2 mM dNTP solution 5 µl 0.2 mM ofeach dNTP25 mM MgCl2*solution3 µl 1.5 mMbcl-2 PCR PrimersSet, 10 pmole/µl2 µl 0.4 µM cDNA** 2 µl ~30 ngTaq DNAPolymerase,5 units/µl1 µl 0.1 units/µl Total volume50 µl -* When using the bcl-2 PCR Primers Set for thefirst time, you may set two additional reactiontubes with a higher and a lower MgCl2concentrations (see Note at the end of thissection).** Optimize this parameter with your own cDNA.3. Mix gently by finger tapping and centrifuge briefly to collect the mixture in the bottom of the tube. Overlay the reaction mixture with 2 drops (~30 µl) of mineral oil to cover the surface of the reaction mixture if not using a thermal cycler with a heated lid. Place the tube in the thermal cycler when the thermal cycler reaches 95 oC, and run the following PCR program. 95 oC for 2 min 94 oC for 45 sec 53 oC for 45 sec x 30 cycles 72 oC for 1.5 min 72 oC for 7 min The amplified DNA can be evaluated by agarose gel electrophoresis.Note: Using different thermal cyclers:For a better detection of the amplified product you may increase the number of amplification cycles. In case you do not see differences in the amount of the amplified DNA fragments, decrease the number of cycles to verify your results.In rare cases, some of the parameters should beoptimized for the specific thermal cycler or cDNA samples. The most frequently adjusted factors are MgCl 2 concentration and annealing temperature. You may prepare three different reactions using MgCl 2 at a concentration of 0.5-3 mM (e.g., 0.5-0.8 mM, 1.5 mM and 3 mM). Optimize the MgCl 2 and/or the annealing temperature on your instrument using the positive control cDNA provided before using your own cDNA.Troubleshooting GuideProblem Cause Solution A PCR component may be missing or degraded. Try to isolate the problematic reagent by replacing it with a fresh one. A checklist is also recommended when assembling reactions.No PCR products cDNA or MgCl 2concentration is not optimal .Optimize the cDNA and MgCl 2 concentrations.Highbackground, smearing or nonspecific bandsIncrease the annealing temperature or decrease the MgCl 2concentration. Another solution for avoiding high background is to decrease the amount of cDNA template used for amplification.Contamination with other DNAUse sterile techniques while performing PCR experiments. cDNA quality is not sufficientUse a different cDNA preparation.Amplifiedproducts are not the correct sizeNon-optimal PCR conditions Optimize PCR conditions especially cDNA and MgCl 2 concentrations and annealing temperature. Poor resolution of products in agarose gelUse 2% agarose gel and increase run time.References1. Oltavi, Z.N., et al ., Cell, 74, 609 (1993)2. Korsmeyer, S.J., Cancer Research (Suppl), 59,1693s (1999)3. Agarwal, N. and Metha, K., Biochem. Biophys.Res. Commun., 230, 251 (1997)4. Aggarwal, S. and Gupta, S., J. Immunol., 160,1627 (1998)†The PCR process is covered by patents owned by Hoffman-LaRoche, Inc. Purchase of this product does not convey a license under these patents.ya 6/00Sigma brand products are sold through Sigma -Aldrich, Inc.Sigma-Aldrich, Inc. warrants that its products conform to the information contained in this and other Sigma -Aldrich publications. Purchaser must determine the suitability of the product(s) for their particular use. Additional terms and conditions may apply.Please see reverse side of the invoice or packing slip.。

Spectroscopic Study of the Interaction between Small Molecules and Large Proteins1. IntroductionThe study of drug-protein interactions is of great importance in drug discovery and development. Understanding how small molecules interact with proteins at the molecular level is crucial for the design of new and more effective drugs. Spectroscopic techniques have proven to be valuable tools in the investigation of these interactions, providing det本人led information about the binding affinity, mode of binding, and structural changes that occur upon binding.2. Spectroscopic Techniques2.1. Fluorescence SpectroscopyFluorescence spectroscopy is widely used in the study of drug-protein interactions due to its high sensitivity and selectivity. By monitoring the changes in the fluorescence emission of either the drug or the protein upon binding, valuable information about the binding affinity and the binding site can be obt本人ned. Additionally, fluorescence quenching studies can provide insights into the proximity and accessibility of specific amino acid residues in the protein's binding site.2.2. UV-Visible SpectroscopyUV-Visible spectroscopy is another powerful tool for the investigation of drug-protein interactions. This technique can be used to monitor changes in the absorption spectra of either the drug or the protein upon binding, providing information about the binding affinity and the stoichiometry of the interaction. Moreover, UV-Visible spectroscopy can be used to study the conformational changes that occur in the protein upon binding to the drug.2.3. Circular Dichroism SpectroscopyCircular dichroism spectroscopy is widely used to investigate the secondary structure of proteins and to monitor conformational changes upon ligand binding. By analyzing the changes in the CD spectra of the protein in the presence of the drug, valuable information about the structural changes induced by the binding can be obt本人ned.2.4. Nuclear Magnetic Resonance SpectroscopyNMR spectroscopy is a powerful technique for the investigation of drug-protein interactions at the atomic level. By analyzing the chemical shifts and the NOE signals of the protein in thepresence of the drug, det本人led information about the binding site and the mode of binding can be obt本人ned. Additionally, NMR can provide insights into the dynamics of the protein upon binding to the drug.3. Applications3.1. Drug DiscoverySpectroscopic studies of drug-protein interactions play a crucial role in drug discovery, providing valuable information about the binding affinity, selectivity, and mode of action of potential drug candidates. By understanding how small molecules interact with their target proteins, researchers can design more potent and specific drugs with fewer side effects.3.2. Protein EngineeringSpectroscopic techniques can also be used to study the effects of mutations and modifications on the binding affinity and specificity of proteins. By analyzing the binding of small molecules to wild-type and mutant proteins, valuable insights into the structure-function relationship of proteins can be obt本人ned.3.3. Biophysical StudiesSpectroscopic studies of drug-protein interactions are also valuable for the characterization of protein-ligandplexes, providing insights into the thermodynamics and kinetics of the binding process. Additionally, these studies can be used to investigate the effects of environmental factors, such as pH, temperature, and ionic strength, on the stability and binding affinity of theplexes.4. Challenges and Future DirectionsWhile spectroscopic techniques have greatly contributed to our understanding of drug-protein interactions, there are still challenges that need to be addressed. For instance, the study of membrane proteins and protein-protein interactions using spectroscopic techniques rem本人ns challenging due to theplexity and heterogeneity of these systems. Additionally, the development of new spectroscopic methods and the integration of spectroscopy with other biophysical andputational approaches will further advance our understanding of drug-protein interactions.In conclusion, spectroscopic studies of drug-protein interactions have greatly contributed to our understanding of how small molecules interact with proteins at the molecular level. Byproviding det本人led information about the binding affinity, mode of binding, and structural changes that occur upon binding, spectroscopic techniques have be valuable tools in drug discovery, protein engineering, and biophysical studies. As technology continues to advance, spectroscopy will play an increasingly important role in the study of drug-protein interactions, leading to the development of more effective and targeted therapeutics.。

美国FDA提交药品和生物制品稳定性文件的指南1987年Ⅰ.导言本指南提供：——举荐稳固性研究的设计，以制订适宜的有效期和储存要求(见Ⅲ部分)。

——举荐为新药临床试验申请(IND's)和生物制品(Ⅳ部分)、新药申请(NDA's)(V部分)和生物制品许可证申请(PLA's)(Ⅵ部分)所提交给药品和生物制品中心(CDB)的稳固性资料和数据。

那个指南是依照21CFRl0．90公布的，申请人可依照指南提交用于人类药品和生物制品的稳固性文件，也可遵循其他方法。

当选择其他方法时，劝说申请人预先与FDA讨论此方法，以防止财务的支出和预备的全部工作提交后被决定不能同意。

目的是提供符合规章要求的方法，如下所列：新药临床试验申请21 CFR 312．23(a)(7)新药申请21 CFR 314．50简略的新药申请21 CFR 314．55生物制品许可证申请21 CFR 601．2增补21 CFR 314．70本指南提供的制定有效期方法来自至少三个不同批次的药品，以保证统计可同意的提议期限可靠。

不管如何样，生产者认识到通过稳固特性的不断评判来进—步确定估量有效期的可靠是重要的，在生产者的稳固性方案方面，如此连续确定有效期将是重点考虑的事。

Ⅱ.定义1．加速试验(Accelerated resting)：通过采纳最不利的贮存条件，对原料药或药物制剂的化学或物理降解增长的速率进行研究设计，其目的是确定动力学参数，以便预言暂定的有效期。

“加速试验”又称“应力试验”。

2．批准的稳固性研究草案(Approved stability study protocol)：编写详细的打算以符合批准的新药申请，同时应用于产生和分析在整个有效期内的可同意的稳固性数据；也能够利用所产生的相近的数据延长有效期。

3．批次(Batch)：按照2l CFR210．3(b)(2)定义，“批次”的意思是一种特定质量的药品或其他物质，在规定的范畴内具有同一的特性和质量，同时是在相同生产周期内按照相同的生产程序生产的产品。

Potassium phosphate dibasic trihydrate Product Number P 5504Store at Room TemperatureProduct DescriptionMolecular Formula: K2HPO4• 3H2OMolecular Weight: 228.2CAS Number: 16788-57-1Synonyms: dipotassium phosphate, potassium biphosphate, sec-potassium phosphate, dipotassium hydrogen phosphate trihydratePotassium phosphate is a reagent with very high buffering capacity that is widely used in molecular biology, biochemistry, and chromatography. Potassium phosphate occurs in several forms: monobasic (KH2PO4), dibasic (K2HPO4), and tribasic (K3PO4). Most neutral potassium phosphate buffer solutions consist of mixtures of the monobasic and dibasic forms to varying degrees, depending on the desired pH. A table for preparation of 0.1 M potassium phosphate buffer at 25 °C using various proportions of potassium phosphate monobasic and potassium phosphate dibasic has been published.1,2 Some limitations of the usefulness of phosphate buffers include their precipitation of Ca2+ and Mg2+, their inhibition of restriction enzyme activity, and their interference in protocols related to DNA ligation and bacterial transformation.1 A study of the effect of freeze-thaw storage cycles on proteins in potassium phosphate and sodium phosphate buffer solutions has been reported.3The use of high concentrations of potassium phosphate in the immobilization of affinity ligands onto epoxide-activated stationary phases has been reviewed.4 A two-phase system of aqueous potassium phosphate and poly(ethylene glycol) for the isolation of E. coliβ-galactosidase and β-galactosidase fusion proteins has been published.5 The quantitation of nonionic surfactants in buffered solutions using strong cation and anion exchange HPLC guard columns and potassium phosphate solution has been investigated.6 Precautions and DisclaimerFor Laboratory Use Only. Not for drug, household or other uses.Preparation InstructionsThis product is soluble in water (100 mg/ml), yielding a clear, colorless solution.References1. Molecular Cloning: A Laboratory Manual, 3rd ed.,Sambrook, J. F., et al., Cold Spring HarborLaboratory Press (Cold Spring Harbor, NY: 2001), p. A1.5.2. Green, A. A., and Hughes, W. L., ProteinFractionation on the Basis of Solubility in Aqueous Solutions of Salts and Organic Solvents. Meth.Enzymol., 1, 67-90 (1955).3. Pikal-Cleland, K. A., et al., Protein denaturationduring freezing and thawing in phosphate buffersystems: monomeric and tetrameric beta-galactosidase. Arch. Biochem. Biophys., 384(2),398-406 (2000).4. Wheatley, J. B., and Schmidt, D. E. Jr., Salt-induced immobilization of affinity ligands ontoepoxide-activated supports. J. Chromatogr. A.,849(1), 1-12 (1999).5. Enfors, S. O., et al., Combined use of extractionand genetic engineering for protein purification:recovery of beta-galactosidase fused proteins.Bioseparation, 1(3-4), 305-310 (1990).6. Pardue, K., and Williams, D., Quantitativedetermination of non-ionic surfactants in proteinsamples, using ion-exchange guard columns.Biotechniques, 14(4), 580-583 (1993).GCY/RXR 1/03Sigma brand products are sold through Sigma-Aldrich, Inc.Sigma-Aldrich, Inc. warrants that its products conform to the information contained in this and other Sigma-Aldrich publications. Purchaser must determine the suitability of the product(s) for their particular use. Additional terms and conditions may apply. Please see reverse side ofthe invoice or packing slip.。

基于HITEMP数据库的分子吸收光谱高精度快速建模方法钱宝健，蔡静*，常海涛，高一凡（航空工业北京长城计量测试技术研究所，北京 100095）摘要：为解决高温环境下分子吸收光谱精确计算的时间复杂性，满足宽光谱测量领域对理论吸收光谱计算的需求，本研究利用Python语言以逐线计算为基础，结合线型函数的简化、线翼截止准则和谱线数据库的优化，建立了基于高温分子吸收参数数据库（High⁃Temperature molecular spectroscopic absorption parameters data⁃base，HITEMP）的分子吸收光谱精确快速计算模型。

以Hartmann⁃Tran线型函数作为吸收光谱标准线型编写部分相关二次速度依赖硬碰撞函数（partially⁃Correlated quadratic⁃Speed⁃Dependent Hard⁃Collision Profile，pCqSDHC），结合复概率函数（Complex Probability Function，CPF）简化模型实现了线型函数的精确快速计算，相较于理论计算模型计算速度提高了20倍。

按照光谱计算残差在10-5量级确定了固定波数截断结合谱线半宽等倍数截断的线翼截止准则。

以阈值线强度10-25 cm-1/(mol∙cm-2)为标准筛选了每100 K温度梯度时的光谱数据，整合得到优化数据库。

在6 500 ~ 8 000 cm-1范围内对水分子的吸收光谱进行计算，并与“”分子气体集成光谱建模网站仿真结果对比，逐线模型的计算误差在10-7量级，优化模型的计算误差在10-5量级，计算速度平均提升25倍。

该模型满足吸收光谱测量中对于理论吸收光谱的高效准确计算，为复杂环境中基于宽调谐、超连续激光吸收光谱的测量研究提供了理论模型基础。

关键词：吸收光谱；HITEMP数据库；线型函数；线翼截止中图分类号：TB9；O433 文献标志码：A 文章编号：1674-5795（2023）05-0039-10Modeling molecular absorption spectra based on the HITEMP databaseQIAN Baojian, CAI Jing*, CHANG Haitao, GAO Yifan(Changcheng Institute of Metrology & Measurement, Beijing 100095, China)Abstract: To address the computational complexity of accurately calculating molecular absorption spectra in high⁃temperature environments and meet the demand for theoretical absorption spectrum calculations in broad⁃spectrum mea⁃surement fields, this study developed a precise and fast calculation model for molecular absorption spectra based on the High⁃Temperature molecular spectroscopic absorption parameters database (HITEMP). The model was implemented us⁃ing Python language, employing a line⁃by⁃line calculation approach combined with simplification of line shape functions, line wing truncation criteria, and optimization of spectral line databases. The Hartmann⁃Tran line shape function was used as the standard absorption spectrum line shape, and partially⁃Correlated quadratic⁃Speed⁃Dependent Hard⁃Collision Pro⁃file (pCqSDHC) was developed for relevant second⁃order velocity⁃dependent hard⁃collision functions. By incorporating the doi：10.11823/j.issn.1674-5795.2023.05.06收稿日期：2023-09-26；修回日期：2023-10-08基金项目：国家“十三五”计量技术基础科研项目（JSJL2020205A003）引用格式：钱宝健，蔡静，常海涛，等.基于HITEMP数据库的分子吸收光谱高精度快速建模方法［J］.计测技术，2023，43（5）：39-48.Citation：QIAN B J，CAI J，CHANG H T，et al.Modeling molecular absorption spectra based on the HITEMP database［J］.Metrology & Measurement Technology，2023，43（5）：39-48.Complex Probability Function (CPF) and simplifying the model, the line shape functions were calculated accurately and rapidly, resulting in a 20⁃fold increase in computational speed compared to theoretical models. The line wing truncation criteria were determined based on the spectral calculation residual at the level of 10-5 and involved the truncation of fixed wavenumbers combined with equal multiple truncations of spectral line half widths. Spectral data for each temperature gradient of 100 K were selected using a threshold line intensity of 10-25 cm-1/(mol∙cm-2) and integrated to create an opti⁃mized database. The absorption spectra of water molecules were calculated within the range of 6 500 ~ 8 000 cm-1 and compared with the simulation results from "", a molecular gas integrated spectral modeling website. The calculation error of the line⁃by⁃line model was at the level of 10-7, while the optimized model achieved a calculation error at the level of 10-5, with an average speed improvement of 25 times. This model enables efficient and accurate calculation of theoretical absorption spectra for absorption spectral measurements and provides a theoretical foundation for measuring studies based on wide⁃tunable and supercontinuum laser absorption spectra in complex environments.Key words: absorption spectrum; HITEMP database; line shape functions; line wing cutoff0　引言分子吸收光谱是一种描述物质分子对特定波长光的吸收能力的图谱，通过测量物质对不同波长光的吸收程度，可以推断物质的组成、浓度、结构和化学性质等重要信息，从而在燃烧诊断［1-2］、温度测量［3-4］、污染物监测［5］等领域中进行定性和定量分析。

Special Property DescriptionsA General PurposeB Low Compression SetE Ethylene Acrylate or (Vamac®)F Fuel Resistant or Fully FluorinatedG Higher Fluorine ContentJ NSF / FDA / WRAS Approvals L Internally LubedM Mil/ AMS Specifications P Low Temperature Flexible or (AFLAS)W Non-Black Compound X CarboxylatedParker O-Ring Compound Numbering SystemsNote: There are two types of nomenclature used to reference Parker O-Ring products. See tables below for description of these types.AFLAS® is a registered trademark of Asahi Glass Co., Ltd.Vamac® is a registered trademark of E.I. du Pont de Nemours & Co.Neoprene® is a registered trademark of DuPont Performance ElastomersMATERIAL OFFERINGPOLYACRYLATE (ACM)ACM (acrylic rubber) has good resistance to mineral oil, oxygen and ozone. The water compatibility and cold flexibility of ACM are considerably worse than withNBR.AA150-70Engines & Transmission Seals -5 to 350Black AA154-75Engines & Transmission Seals-5 to 350BlackETHYLENE ACRYLATE (AEM)Ethylene acrylate is a mixed polymer of ethylene, methyl acrylate and a small amount of carboxylated cure-site monomer. Developed as a lower-temperature version of Polyacrylate, but swells slightly more. Polymer is sold under the tradename VAMAC®. AE152-70Transmission Applications-40 to 325Black AE153-75Transmission Applications, Internally Lubed-40 to 325BlackBUTYL RUBBER (IIR)Butyl rubber (isobutylene-isoprene rubber or IIR) has a very low permeabilityrate and good electrical properties, but poor short-term rebound.B0318-70General Purpose-75 to 250Black B0612-70Vacuum, Low Compression Set-75 to 250BlackPOLYCHLOROPRENE RUBBER (CR)Also known by the tradename Neoprene®, polychloroprene was the first synthetic rubber and exhibits generally good ozone, aging, and chemical resistance. It has good mechanical properties over a wide temperature range. C0267-50MIL-G-1149 Ty I Cl I, AMS 3208, Low Temperature-60 to 250Black C0557-70Drive Belt Applications, General Purpose -35 to 250Black C0873-70Refrigerant Gases, Low Extractibles, Low Compression Set -35 to 225Black C0944-70General Purpose-35 to 250RedTEMP. RANGECOMPOUND NO. RECOMMENDED FOR (Degrees° F) COLORC1124-70AMS 3209, Low Temperature-60 to 250Black C1276-70Super Neoprene, Low Compression Set -35 to 250Black CL172-70 Internally Lubed-35 to 225Black C1278-80Super Neoprene, Low Compression Set-35 to 250BlackEPICHLOROHYDRIN (ECO)Epichlorohydrin is a special-purpose seal material with good resistance to both refrigerant gases and gasoline, as well as inherent resistance to atmospheric ozone.YB146-75Refrigerant Gases, Gasoline, O-zone Resistant-31 to 250BlackETHYLENE PROPYLENE RUBBER (EPM, EPR, EPDM)EPM (EPR) is a copolymer of ethylene and propylene. EPDM is a terpolymer of ethylene, propylene, and a diene third monomer used for cross-linking.E1100-50 General Purpose -70 to 250 Black EA454-50 (3575) UL Listed -70 to 250 Black E1157-60 General Purpose -70 to 250 Black E1561-60 NSF 61, KTW, WRAS -70 to 250 Black E0751-65 Drive Belt Applications -70 to 250 Black E0603-70 General Purpose -70 to 250 Black E0667-70 Auto Disc Brakes -70 to 250 Black E0803-70 General Purpose -70 to 250 Black E1022-70 Internally Lubed, Brakes -70 to 250 Black E1028-70 FDA -70 to 250 Black E1244-70 NSF 61, Internally Lubed -70 to 250 Black E1549-70 NSF 61, WRAS, KTW, FDA -70 to 250 Black E1583-70 NSF 51, NSF 61, -70 to 250 Black Internally Lubed E3609-70 NSF 51, NSF 61, WRAS, -70 to 250 Black KTW, FDA, USP Class VI EB152-70 (3407) General Purpose -70 to 250 Black EJ273-70 Chloramine Resistant -70 to 250 Black EJ274-70 Internally lubed, NSF 61 -70 to 250 Black Chloramine Resistant E3609-70 NSF 51 & 61, WRAS, FDA, -70 to 250 Black USP Class VI, ISO 10993 USP <87> E0740-75 Nuclear Applications -70 to 250 BlackTEMP. RANGE COMPOUND NO. RECOMMENDED FOR (Degrees° F)COLORMATERIAL OFFERINGE0515-80 NAS 1613 Rev 2 -70 to 250 Black E0540-80 General Purpose -70 to 250 Black E0893-80 General Purpose -70 to 250 Purple E1267-80 NAS 1613 Rev 5 -70 to 250 Black E0652-90 General Purpose, -60 to 250 BlackBack-Up RingsE0962-90 Excellent Steam to 500° F , -60 to 250BlackED ResistantFLUOROSILICONE (FVMQ)Fluorosilicone is a silicone polymer chains with fluorinated side-chains for improved oil and fuel resistance. The mechanical and physical properties arevery similar to those of silicone.LM151-50 (11645) General Purpose -100 to 350 Blue LM158-60 AMS-R-25988, TY 1, CL 1, -100 to 350 Blue GR 60, AMS 3325LA163-70 General Purpose -100 to 350 Greene LM100-70 MIL-DTL-25988, TY1, CL 1 -100 to 350 Blue Gr 70, UL Listed LM159-70 MIL-DTL-25988, TY 1, CL 1, -100 to 350 Blue GR 70 L1120-70 MIL-DTL-25988, TY I, -100 to 350 Blue CL I, GR 70, UL listed L1077-75 MIL-DTL-25988, -90 to 350 Blue TY I, CL III, GR 75 40713-75 Automotive Fuel Quick -90 to 350 Yellow Disconnects LM160-80 MIL-DTL-25988, TY 1, CL 1, -90 to 350 Blue GR 80 L1186-80 PTFE Loaded -85 to 350 RustTEMP. RANGECOMPOUND NO. RECOMMENDED FOR (Degrees° F) COLORTEMP. RANGE COMPOUND NO. RECOMMENDED FOR (Degrees° F) COLORACRYLONITRILE-BUTADIENE (NBR)Nitrile rubber (NBR) is the general term for acrylonitrile-butadiene terpolymer. The acrylonitrile content of nitrile sealing compounds varies considerably (18 to 50%.) Polymers with higher ACN content exhibit less swell in gasoline and aromatic solvents, while lower ACN polymers exhibit better compression set and low temperature flexibility. Polymer is also called Buna-N.GENERAL PURPOSEN0545-40 AMS 3201 -45 to 225 Black NA192-50 General Purpose, -45 to 250 BlackNon-BloomingN0525-60 AMS 3212, AMS 3220 -25 to 250 Black N1219-60 NSF 51, FDA -30 to 225 Black N0287-70 Synthetic Lubricant -35 to 250 BlackResistant, AMS 7272N0674-70 General Purpose -30 to 250 BlackMIL-G-21569, Cl I,UL listedN0757-70 NSF 61, UL Listed -30 to 225 Black N1069-70 FDA -30 to 180 Black N1220-70 NSF 51, FDA -30 to 225 Black N1470-70 General Purpose, X-rings -40 to 225 Black N1471-70 General Purpose -40 to 250 Black N1499-70 General Purpose, UL -30 to 250 Black N1510-70 NSF 61 -30 to 225 Black N1517-70 NSF 61 -30 to 225 Black N1527-70 UL Listed -30 to 225 Black N0508-75 FDA, USDA -30 to 180 Black NA155-80 (7538) Abrasion Resistant -25 to 250 Black N0300-90 Back Up Rings -40 to 180 Black N0552-90 General Purpose -30 to 250 Black N1444-90 Parbaks only -30 to 250 Black N1490-90 General Purpose -30 to 250 Black LOW SETN0299-50 UL listed -55 to 225 Black NL151-50 Internally Lubed -55 to 225 Black N0406-60 Low Temperature -55 to 225 Black NM506-65 AMS 7271 -70 to 180 Black N0103-70 Low Temperature -55 to 225 Black N0602-70 AMS-P-5315, -70 to 180 BlackLow TemperatureNM072-70 AMS-R-7362 -60 to 180 BlackMATERIAL OFFERINGNM304-75 MIL-DTL-25732 -66 to 225/250 Black N0756-75 AMS-P-83461, -65 to 250/275 BlackLow Comp. Set, CNG Applications N0507-90 AMS-P-5510 -65 to 180 BlackLow Temperature, CNG ApplicationsHigh ACN/Low Swell N0497-70 Low Swell, SAE 1120R1 CL2, -35 to 212 BlackUL ListedN1500-75 Low Swell, Fuel Applications -35 to 212 BlackUL ListedImproved Dynamic Properties NL151-50 Internally Lubed -55 to 225 Black NW163-70 Internally Lubed -30 to 225 Rust N0818-70 Internally Lubed -30 to 250 Black N0750-80 Carboxylated (good wear -25 to 250 Black resistance)N1090-85 Carboxylated (good wear -25 to 225Blackresistance), Internally LubedHYDROGENATED NITRILE (HNBR, HSN)Hydrogenated nitrile was developed as an air-resistant variant of nitrile rubber. In HNBR, the carbon-carbon double bonds in the main polymer chain are saturated with hydrogen atoms in a process called “hydrogenation” that improves the material’s thermal stability and oxidation resistance.KB190-50 (21705) Automotive Applications -25 to 300/325 Black N1173-70 General Purpose -25 to 300/325 Black N1239-70 Refrigerants -25 to 300/325 Red KA157-70 (21407) General Purpose -30 to 300/325 Black KA158-70 Low Temperature -40 to 300/325 Black KA174-75 (21107) 75 Duro, General Purpose -25 to 300/325 Black N1231-80 RGD Resistand, -25 to 300/325 Black Automotive Refrigerants KA453-80 (21508) Low Swell, ED Resistant -25 to 300/325 Black KB162-80 (21378) High Temp. Hydraulics -25 to 300/325 Black KA183-85 Low Temp. -55 to 300/320 Black KB163-90 (21379) High Temp. Hydraulics -25 to 300/325 Black ED Resistant N4007-95 Extrusion Resistant, -25 to 300/325 Black ED ResistantTEMP. RANGECOMPOUND NO. RECOMMENDED FOR (Degrees° F) COLORTEMP. RANGE COMPOUND NO. RECOMMENDED FOR (Degrees° F) COLORPOLYURETHANE (AU, EU)Polyurethane elastomers have excellent wear resistance, high tensile strength and high elasticity in comparison with any other elastomers. Permeability is good and comparable with butyl. Millable urethanes should not be confused with thermoplastic urethanes, which are generally harder, less flexible, and have slightly better wear resistance.P0642-70Drive Belt Applications, Millable-40 to 180Black SILICONE RUBBER (VMQ, PVMQ)Silicones possess good insulating properties and tends to be physiologically neutral. However, silicone elastomers have relatively low tensile strength, poor tear and wear resistance.S0469-40 AMS 3301 -75 to 400 Rust S0802-40 FDA -60 to 400 White S0595-50 AMS 3302 -70 to 400 Rust S0899-50 ZZ-R-765 CL 1a, 1b, 2a, 2b -103 to 400 RustGR 50SA150-50 Low Organic Extractibles -65 to 400 Trans S0317-60 FDA, USDA, USP CL VI -103 to 450 Rust S0613-60 ZZ-R-765 Cl 2b, Gr 60, -60 to 450 RustAMS 3303S0383-70 ZZ-R-765 Cl 1a, 1b, -175 to 400 RustGr 70, AMS 3337S0455-70 High Temp. -65 to 450/500 Rust S0604-70 ZZ-R-765 Cl 2a, 2b, Gr 70 -65 to 450 RustAMS 3304, AMS 3357MIL-G-21569S1138-70 FDA -60 to 400 Rust S1224-70 ZZ-R-765 Cl 2a, 2b, Gr 70 -65 to 450 RustAMS 3304, AMS 3357MIL--21569SM355-75 AMS 7267, FDA, USDA -60 to 450 Rust S0614-80 ZZ-R-765 Cl 2a, 2b -65 to 450 RustGr 80, AMS 3305MATERIAL OFFERINGFLUOROCARBON (FKM, FPM)Fluorocarbon (FKM) has excellent resistance to high temperature and a broad range of chemicals. Permeability and compression set are excellent.General Purpose V0986-50 General Purpose -15 to 400 Brown V0763-60 General Purpose -15 to 400 Brown V0769-60General Purpose -15 to 400 Black VA150-65 (19356) General Purpose -15 to 400 Black V0680-70 FDA, USDA, NSF 51 -15 to 400 Red V0747-75 AMS-R-83248, TY I, CL I, -15 to 400BlackUL listed V0848-75 PTFE Loaded-15 to 400 Black V0884-75 General Purpose, UL listed -15 to 400 Brown V1164-75 Low Set, AMS 7276, -15 to 400BlackAMS-R-83248, TY I, CL I V1226-75 Low Set,-15 to 400BrownAMS 7276, UL listed,AMS-R-83248, TY I, CL I V1436-75 General Purpose, -15 to 400 BlackUL ListedV1475-75 General Purpose -15 to 400 Black V1476-75General Purpose-15 to 400 Brown VA151-75 (19357) General Purpose, UL Listed -15 to 400 Black VA203-75 (16737) Extrusion Resistant, -15 to 400 BlackDiesel Fuel Injectors VM100-75 Low Compression Set, -15 to 400 BlackAMS 7276VW153-75 (16207) General Purpose-15 to 400 Brown VW173-75 (19457) Automotive Applications -15 to 400 Green VA163-80 (19318) Internally Lubed-15 to 400 Black V0709-90 AMS-R-83248, TY I, Cl II -15 to 400BlackAMS 7259V0894-90 General Purpose -15 to 400 Brown V1411-90 General Purpose -15 to 400 Black V1412-90General Purpose -15 to 400 Brown VA153-90 (19359) General Purpose -15 to 400 Black VW155-90 (16129) General Purpose -15 to 400 Green V1238-95 Extrusion Resistant,-15 to 400BlackExplosive DecompressionResistantTEMP. RANGECOMPOUND NO. RECOMMENDED FOR (Degrees° F) COLORTEMP. RANGE COMPOUND NO. RECOMMENDED FOR (Degrees° F) COLORImproved Chemical ResistanceV1262-65 Low Swell-Flex Fuel Blends -15 to 400 BlackUL lIstedVW252-65 Low Swell -15 to 400 Green VB185-70 Acid Resistant, Steam -15 to 400 Black 16327-75 Automotive Fuel, Good -15 to 400 GreenPermeation ResistanceV1163-75 "GFLT" Type, UL Listed -35 to 400 Black V1260-75 Very Chemically Resistant -15 to 400 Black V1263-75 Low Swell, Flex Fuel Blends, -15 to 400 BlackUL ListedV1289-75 AMS 7379, Extreme Low -55 to 400 BlackTemp (-40 TR-10)VB153-75 Good Compression Set, -15 to 400 BlackFuelsVG162-75 Good Fuel Resistance, -15 to 400 Black"GF" TypeVW263-75 Biodiesel Reistant -15 to 400 Brown V1274-80 USP Class VI, ISO 10993, -15 to 400 BlackLow SwellVG286-80 Fuel Injectors, Good -45 to 400 BlackMethanol & BiodieselResistanceVP104-85 Base Resistant +10 to 400 Black Improved Low TemperatureV1163-75 "GFLT" Type, UL Listed -35 to 400 Black VG292-75 Engine Coolant & Biodiesel -40 to 400 BlackResistantVM125-75 "GLT" Type, AMS-R-83485 -40 to 400 BlackAMS 7287, Low SetVM128-75 "GLT" Type, AMS-R-83485 -40 to 400 Black VM835-75 “GLT Type”, AMS-R-83485 -40 to 400 Black Extreme Low TemperatureVG286-80 Fuel Injectors, Good -45 to 400 BlackMethanol & BiodieselResistanceV1289-75 AMS 7379, Good Methanol -55 to 400 BlackResistanceMATERIAL OFFERINGVX065-75 Extreme Low Temperature, -65 to400 BlackLow Com. SetVG109-90 ISO 23936 RGD & -45 to 400 BlackExtrusion Resistant,High Pressure CNGApplicationsVX165-90 Extreme Low Temperature, -55 to 400 BlackLow Comp. Set TETRAFLUOROETHYLENE - PROPYLENE (AFLAS)This material is a copolymer of TFE and propylene. Its chemical resistance isexcellent across a wide range of aggressive media. Polymer is sold under the tradename Aflas®.V1006-75AMS 725525 to 450BlackVP101-80General Purpose25 to 450BlackVP102-80LowCompression Set15 to 450BlackV1041-85RGD Resistant,GeneralPurpose,NORSOK M71015 to 450BlackVP103-90ExtrusionResistant25 to 450BlackHIGH PERFORMANCE FLUOROELASTOMER (HiFluor)Hifluor is Parker’s tradename for high performance fluoroelastomers – materialsthat “bridge the gap” between traditional fluorocarbon and perfluoroelastomer.HF355-65USP Class VI, Extreme LowExtractibles-15 to 400TranslucentV3819-75Chemically Resistant, LowCompression Set-15 to 400BlackHF391-75Extreme Resistance to PlasmaEtching-15 to 375BlueHF359-80Resistant to Plasma Etch, LowMetal Ions -15 to 400Tan TEMP. RANGECOMPOUND NO. RECOMMENDED FOR (Degrees° F) COLOR11Parker Hannifin Corporation • O-Ring Division2360 Palumbo Drive, Lexington, KY 40509Phone: (859) 269-2351 • Fax: (859) 335-5128ORD 5712O-Ring Material Offering GuidePERFLUOROELASTOMER (ULTRA™)Perfluoroelastomer is a rubber version of PTFE. Available from Parker under the tradenames Parofluor™ and Parofluor ULTRA™.FF374-60Low Particle Generation, Low Metal Ion Content 5 to 608PurpleFF354-65Low Closure Force5 to 608White FF102-75Acid Resistant, General Purpose 5 to 525Black FF106-75General Purpose, Low Cost 5 to 500Black FF200-75Low Comp Set, AMS7257, FDA 5 to 608Black FF302-75Etch Resistant, Low Metal Ions 5 to 608Brown FF350-75Plasma, High urity, FDA, USP Class VI5 to 608White FF352-75General Purpose, Etch Resistant 5 to 608White FF370-75Low Particle Generation, Low Metal Ion Content 5 to 608Black FF400-75Extreme Low Temperature -40 to 500Black FF500-75Broad Chemcial Resistance, FDA 5 to 550Black FF580-75Steam/Amine/Base Resistant, USP Class VI5 to 525Black V8545-75FDA, General Purpose 5 to 572Black FF376-80Low Particle Generation, Low Metal Ion Content5 to 608Black FF202-90Low Compression Set, Extrusion Resistant Pressure 5 to 608Black FF582-90Pressure5 to 525Black V8581-90Plasma Etch Resistant, Low Stiction5 to 550White V8588-90Extrusion & RGD Resistant5 to 572BlackTEMP. RANGE COMPOUND NO. RECOMMENDED FOR (Degrees° F)COLORNumbers in ( ) next to the Parker compound reference old Wynn's Precision numbers.Hifluor™ is a registered trademark of the Parker Hannifin Corporation ULTRA™ is a registered trademark of the Parker Hannifin Corporation。