AP650

表733-11-I 编号：AIP650-AX-BJ-78AIP650LED驱动控制/键盘扫描专用集成电路产品说明书说明书发行履历：版本发行时间新制/修订内容2010-01-A 2010-01 更换新模板2012-01-B1 2012-01 增加说明书编号及发行履历第 1 页共 12 页版本：2012-01-B11、概 述AIP650是一种带键盘扫描电路接口的LED 驱动控制专用电路。

内部集成有MCU 输入输出控制数字接口、数据锁存器、LED 驱动、键盘扫描、辉度调节等电路。

本芯片性能稳定、质量可靠、抗干扰能力强，可适应于24小时长期连续工作的应用场合。

其主要特点如下： ● 显示模式：8段×4位● 段驱动电流不小于25mA ，字驱动电流不小于150mA. ● 提供8级亮度控制 ● 键盘扫描：7×4bit ● 高速两线式串行接口 ● 内置时钟振荡电路 ● 内置上电复位电路 ● 支持3V －5.5V 电源电压● 使用时VCC 端建议加104电容，且电容尽量靠近AIP650的VCC 端口（小于2cm ） ● 封装形式：DIP16/SOP162、引脚排列图及引脚说明2.1、引脚排列图第 2 页 共 12 页版本：2012-01-B1DP/KP G/KI7F/KI6E/KI5D/KI4C/KI3VCC B/KI21211DIG1CLK DAT GND DIG2DIG3DIG4A/KI11615141312345678910第 3 页 共 12 页版本：2012-01-B12.2、引脚说明 引脚符 号引 脚 名 称功 能1 DIG1 位/键扫描输出 LED 位驱动输出，低电平有效，及作为键盘扫描输出，高电平有效。

2 CLK 时钟输入 2线串行接口的数据时钟输入，内置上拉电阻。

3 DAT 数据输入/输出 2线串行接口的数据输入输出，为内置上拉开漏模式。

4 GND接地端 接地5 DIG2位/键扫描输出 LED 位驱动输出，低电平有效，及作为键盘扫描输出，高电平有效。

精神技能名称效果消费SP偵察调查对方的状态以及各种数据。

1集中一个回合以内，使命中率和回避率提高30％。

15ひらめき受到敌人的攻击可以完全回避不受伤害，只限一次。

10必中在一个回合内，命中率达到100％，攻击必定会命中。

但是如果对方使用了技能“ひらめき”则没有效果。

20てかげん使用了此技能后不会直接击溃敌人，会给敌人留下10HP，但是，如果技量数值不比对方高的话，改技能无效。

10加速移动力+3，只限一次，在移动之前一直有效。

5突撃1个回合以内，移动后可以使用除了Map外的武器（P武器）。

25応援指定的角色在下一场战斗内获得2倍的经验值。

20鉄壁1个回合以内，自己的装甲值增长到2倍。

30脱力指定的敌人角色的气力值-10。

40熱血可以给予敌人2倍的攻击伤害，只限一次，可以和会心攻击的效果叠加。

40魂可以给予敌人2.5倍的攻击伤害，只限一次，不可以和“熱血”叠加使用。

60献身给予指定的战机的驾驶员回复10点的SP，如果战机上有多名驾驶员，则全员都可以回复10点SP。

30努力在下一场战斗内获得2倍的经验值。

15幸運在下一场战斗内获得2倍的金钱。

40祝福指定的角色在下一场战斗内获得2倍的金钱。

45自爆发生自爆，无视对方的防御力，按照自己的剩余HP值给予身边角色（包含我方人员）相同的伤害值。

1気合对自己的气力值+10。

50激励对指定的我方战机的气力值+10 70根性回复自己最大HP值的30％。

20ド根性完全回复自己的HP值。

40信頼给予指定的角色回复最大HP值的30％。

30再動可以使一名行动结束的角色再次行动。

90覚醒一次可以连续行动多次。

50奇襲同时附加“加速”，“熱血”，“ひらめき”，“必中”等多种属性的技能。

60補給回复指定角色的最大EN值和剩余弹药数值，但是被使用技能的角色的气力值会－１0。

70復活可以令行动不能的角色复活。

100奇跡同时给予“気合×３，加速，閃き，必中，努力，幸運，魂”等多种效果，只限一次。

AlphaScreen®SureFire®STAT3 (p-Tyr705) Assay KitsManualAssay Points Catalog #500TGRS3S50010 000TGRS3S10K50 000TGRS3S50KFor Research Use OnlyResearch Reagents for Research Purposes OnlyGeneral Information on the AlphaScreen® SureFire® p-STAT3 assayThe AlphaScreen® SureFire® p-STAT3 assay is used to measure the phosphorylation of endogenous STAT3αand STAT3βin cellular lysates. The assay is an ideal system for the screening of both modulators of receptor activation (e.g. agonists and antagonists) as well as agents acting intracellularly, such as small molecule inhibitors of upstream events. The assay will measure STAT3 phosphorylation by either cloned or endogenous receptors, and can be applied to primary cells.This assay eliminates the need for laborious techniques, such as Western blotting or conventional ELISA. It is a homogeneous assay, in that no sample washing steps are required, which allows for minimal handling, short assay times, and robotic operation if desired. The assay utilizes the bead-based Alpha Technology, and requires an Alpha Technology-compatible plate reader.Alpha Technology AlphaScreen® SureFire® Assay PrincipleAlphaScreen® SureFire® technology allows the detection of phosphorylated proteins in cellular lysates in a highly sensitive, quantitative and user friendly assay. In these assays, sandwich antibody complexes, which are only formed in the presence of analyte, are captured by AlphaScreen donor and acceptor beads, bringing them into close proximity. The excitation of the donor bead provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in the emission of light at 520-620nm.Kit-Specificity informationThis assay kit contains antibodies which recognize the phospho-Tyr705-epitope, and a distal epitope on STAT3αand STAT3β. The proteins detected by this kit correspond to GenBank Accessions NP_644805 and NP_998827.These antibodies recognize STAT3 of human, mouse and rat origin. Other species should be tested on a case-by-case basis.Kit ContentsKit Size500 points10,000 points50,000 pointsLysis buffer (5X) 1 x 10 mL 4 x 60 mL 3 x 400 mL Activation buffer 1 x 2 mL 1 x 60 mL 1 x 300 mL Reaction buffer 1 x 2.6 mL 1 x 45 mL 1 x 225 mL Dilution buffer 1 x 1.5 mL1 x 25 mL2 x 60 mLPositive Control Lysate 1 tube to be re-dissolved in 250 µL H 2O Negative Control Lysate1 tube to be re-dissolved in 250 µL H 2OStorage conditions upon receiptThe kit buffers e.g. 5X Lysis buffer, Activation buffer and Reaction buffer should be stored at 4°C. DO NOT freeze the kit buffers – the Reaction buffer contains antibodies and freeze/thaw cycles can lead to a loss of activity.Materials Required But Not ProvidedThe AlphaScreen SureFire assay kits are optimized to work with AlphaScreen Protein A general IgG detection beads. These are available separately from PerkinElmer. The AlphaScreen Protein A general IgG detection kits contain a biotinylated rabbit IgG control, which can be used to test the instrument settings and bead performance.ItemSuggested source Catalog # Size Protein A general IgG detection kit(contains the Acceptor and Donor Beads) PerkinElmer Inc. 6760617C 6760617M 6760617R 500 pt 10,000 pt 50,000 pt Proxiplate™-384 Plus, white, shallow well assay platePerkinElmer Inc. 6008280 6008289 50/box 200/box Optiplate™-384 Plus, white, assay plate PerkinElmer Inc. 6007290 6007299 50/box 200/box TopSeal-A 384, clear adhesive sealing film PerkinElmer Inc. 6050185100/boxEnvision® or Enspire® Alpha-readerPerkinElmer Inc.--Buffer preparation and subsequent storage conditions5X Lysis bufferStore 5X Lysis buffer at 4°C. For assay, dilute 5-fold in water immediately prior to use. Discard unused buffer. Activation bufferPrecipitation will occur during storage 4°C. To re-dissolve, warm to 37°C and mix. Alternatively, Activation buffer can be stored at room temperature with no loss in activity.Reaction buffer*Keep on ice while in use. Do not freeze.Once diluted discard unused reaction buffer. AlphaScreen ® Protein A IgG KitStore at 4°C in the dark.Acceptor Mix(Reaction buffer + Activation buffer +AlphaScreen ® Acceptor beads)Immediately prior to use, dilute Activation buffer B 5-fold in Reaction buffer (e.g. take 98 μL Activation buffer and dilute in 392 μL Reaction buffer).Dilute Acceptor beads 50-fold in Acceptor mix (e.g. add10 μL Acceptor beads to 490 μL of premixed Reactionbuffer + Activation buffer).The Acceptor mix should be used immediately for best results. Excess mix should be discarded.Donor Mix**(Dilution buffer + AlphaScreen ®Donor beads)Immediately prior to use, dilute Donor beads 20-fold in Dilution buffer (e.g. add 10 μL Don or beads to 190 μLDilution buffer).The Donor mix should be used immediately for best results. Excess mix should be discarded.Assay Control lysateAfter reconstitution in 250 μL water, lysates should be frozen at -20°C in single use aliquots and used within 1 month.* Do not vortex the Reaction buffer, as vigorous mixing can damage some antibodies. ** Prepare and use Donor Mix under low-light conditions.Control Lysate informationControl lysates are prepared from A431 cells (ATCC #CRL-1555) at a concentration of approximately 1 mg/mL. The controls are supplied lyophilized, and should be reconstituted in either dd H 2O or MilliQ® H 2O. Once reconstituted, lysates should be stored frozen in single use aliquots.Negative Lysate: Prepared from A431 cells treated with EGF receptor inhibitor (2 μM AG1478)for 2 hours prior to lysis.Positive Lysate: Prepared from A431 cells treated with 200 ng/mL EGF for 10 minutes.p-STAT3 AlphaScreen® SureFire® Assay ProtocolsA. 2-Plate Assay - assay protocol for adherent cellsCell Seeding1. Seed cell s (200 μL of cells for 96 well plates, 50 μL for 384 well plates) in tissue culture plates. Incubate at 37°C overnight in serum-containing media.Cell Treatment2. Remove culture media, and stimulate the cells with 50 μL agonists prepared in serum-free media (25 µL for 384-well plates). (If testing antagonists, prior to stimulation remove culture medium and replace with 50 μL serum-free media containing antagonists (25 µL for 384-well plates)). Return cells to 37°C incubator for desired time. 1 hour is often sufficient for signal transduction inhibitors, and 5 minutes for receptor agonists.Note: Peptidic agonists and antagonists can often stick to plastic surfaces. To minimize this effect, dilute in serum-free media containing a suitable carrier protein (e.g. 0.1% IgG free BSA - Jackson Immunoresearch Cat #001-000-161).Lysate Preparation5. To lyse cells, remove medium from wells, and add freshly prepared 1X Lysis Buffer(50-100 μL fora 96 well plate, 25 μL for a 384 well plate). Agitate on a plate shaker (~350 rpm) for 10 minutes at room temperature.6. Take 4 μL of the lysate and transfer to a 384-well Proxiplate™ for assay. (Add 4 μL Control lysates to separate wells if required).SureFire Assay7. Add 5 μL of Acceptor Mix to wells. Seal plate with Topseal-A adhesive film, and incubate for 2 hours at room temperature.8. Add 2 μL of Donor Mix to wells under subdued light. Seal plate with Topseal-A adhesive film, and cover plate with foil. Incubate for 2 hours at room temperature.Note: Longer incubation may give greater sensitivity. Plates can be incubated overnight if required.9. Read plate on an Alpha Technology-compatible plate reader, using standard AlphaScreen settings.B. 1 Plate Assay - assay protocol for non-adherent cells, and for high-throughput applications. Note: the larger volumes required using this assay will result in achieving less assay points per kit. Cell Seeding1. Harvest cells by centrifugation, and re-suspend cells in HBSS at a suitable cell density. We recommend 107cells/mL as a starting point. Seed 4 μL of cells/well into a 384-well culture plate.2. If using test agents/inhibitors, add 2 μL/well of 4X inhibitors prepared in HBSS.Note: Peptidic agonists and antagonists can often stick to plastic surfaces. To minimize this effect, dilute in serum-free media containing a suitable carrier protein (e.g. 0.1% IgG free BSA - Jackson Immunoresearch Cat #001-000-161).3. Return cells to incubator at 37°C for 1-2 hours.Cell Treatment4. Stimulate cells with agonists by addition of 2 μL/well of 4X agonist stock in HBSS containing 0.1% BSA. The final volume in the wells should be 8 μL. (if no antagonists were used in step 2, stimulate the cells with 4 μL/well of 2X agonist, to give a final volume in the wells of 8 μL.)Lysate Preparation5. To lyse the cells, add 2 μL/well 5X Lysis buffer.(Add 10 μL control lysates to separate wells if required)SureFire Assay6. Add 8 μL of Acceptor Mix to wells. Seal plate with Topseal-A adhesive film, and incubate for 2 hours at room temperature.7. Add 3 μL of Donor Mix to wells under subdued light. Seal plate with Topseal-A adhesive film, and cover plate with foil. Incubate for 2 hours at room temperature.Note: Longer incubation may give greater sensitivity. Plates can be incubated overnight if required.8. Read plate on an Alpha Technology-compatible plate reader, using standard AlphaScreen settings.Representative DataWestern blot analysis (35 μg protein/lane) of phospho-STAT3 in lysates generated from either unstimulated (-) or IL6-stimulated (+) THP-1 cells, or the AlphaScreen SureFire assay (3.5 μg protein/well), using the standard 2-plate protocol.THP-1 cells (ATCC Cat# TIB-202) were harvested from growing flasks, resuspended in HBSS, and seeded at 25K cells/well in 384-well microplates. Cells were incubated for 2 hours at 37°C, and stimulated with IL-6 for 10 minutes at room temperature (left) or pre-treated with JAK inhibitor I for 2 hours at 37°C, then stimulated with IL-6 (right). Cells were lysed with 5X Lysis buffer with shaking at RT for 10 minutes, and analyzed for phospho-STAT3 using the standard 1-plate protocol.Frequently Asked Questions & TroubleshootingFor comprehensive information on assay optimization and troubleshooting, please refer to the following resources:▪ Guide to AlphaScreen® SureFire ® assay optimization ▪ AlphaScreen® SureFire ® user guideTo download these resources, and other related technical information, visit /category/alpha-surefire-kitsFor general information on AlphaScreen® SureFire ® assays, visit - +Customer CareTo contact the customer care team, please visit /ServiceCallFor more information regarding related AlphaScreen® SureFire® products andprotocols refer to:PerkinElmer web site: TGR BioSciences website: FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.This product is not for resale or distribution except by authorized distributors.LIMITED WARRANTY: PerkinElmer, Inc. warrants that, at the time of shipment, the products sold by it are free from defects in material and workmanship and conform to specifications which accompany the product. PerkinElmer Inc. makes no other warranty, express or implied with respect to the products, including any warranty of merchantability or fitness for any particular purpose. Notification of any breach of warranty must be made within 60 days of receipt unless provided in writing by PerkinElmer Inc. No claim shall be honored if the customer fails to notify PerkinElmer Inc. within the period specified. The sole and exclusive remedy of the customer for any liability of PerkinElmer Inc. of any kind including liability based upon warranty (express or implied whether contained herein or elsewhere), strict liability contract or otherwise is limited to the replacement of the goods or the refunds of the invoice price of goods. PerkinElmer Inc. shall not in any case be liable for special, incidental or consequential damages of any kind.。

发烧音响有问有答问：请问Mission Pilastro报价。

用什么功放推？答：此扬声器的定价大概30万左右，建议用PASS LABS X350推，或者BRYSTON的4B、7B或者14B来推，也可以用MARK LEVENSON 4系列来推。

问：天仙配视频三线RCA有没有5米长的？价钱是多少？望给予回复多谢！！答：天仙配视频三线RCA线有5米长的，但需要订货，价格大约1万5千元人民币左右，详情请月国内代理广州保捷极品影音行联系，他们的电话是问：在下有几个问题请教。

现有器材:功放.马兰士pm-16;cd机.TEAC 20;音箱.AE100;全套超时空方心铜线材.听音广范,现想升级音箱,心意的有PROAC 50S,PROAC 2000S,B&W CDM 1NT..请推荐。

但关于马兰于PM-16功放,本人翻查了大量的音响刊物及上网查讯,却只有大量关于马兰士pm-14及pm-17的评论。

现请主持点评马兰士pm-16功放的声色,特点及与pm-14和pm-17有何不同？达到何等级别？另请推荐一台中低价位的LP唱盘。

望主持先生能抽空解答我的难题。

答：建议用PM-16推奥地利的维也纳的海顿扬声器，此箱的价格约8800元，或者选丹麦的SEK的S11，此箱价格约14800元。

这样的搭配比较合理，声音的表现也比较完美。

LP转盘可以考虑下美国的音乐教堂的唱盘。

问：请问BRYSTON 7Bst 和7Bst Pro ，BP-25前级的价钱，有现货吗？答：这套器材全部大概7万元左右，具体要与代理那边联系下才知道有没有现货，他们的电话是，。

问：本人想查询英国ATC音箱的中国(广州市)代理商是谁? 答：他们的代理的电话是，问：我于今年购得森海塞尔HD580耳机一副，望推荐耳放（国产，进口）及讯号线，现看中科颂U5及VHL 102D，不知此番搭配是否妥当。

另外，不知国产的滤波器效果如何望指点一二，平日喜欢听人声和小的作品，如钢琴、小号的小段，听感要求细节丰富，富有音乐感和层次感，对低频要求不高答：耳放可以用英国瑟顿SUGDEN或者意大利的BLUE NOTE的产品。