Excalor 易克热

OMEGARH62CHYGROMETEROMEGAnet On-Line Service Internet e-mail **************For immediate technical or application assistance:USA and Canada:Mexico and Latin America:Sales Service: 1-800-826-6342 / 1-800-TC-OMEGA Tel: (95) 800-TC-OMEGA Customer Service: 1-800-622-2378 / 1-800-622-BEST FAX: (95) 203-359-7807Engineering Service: 1-800-872-9436 / 1-800-USA-WHEN En Español: (203) 359-7803TELEX: 996404 EASYLINK: 62968934 CABLE: OMEGA e-mail:*****************Servicing North America:USA: ISO 9001 Certified Canada:One Omega Drive, Box 4047976 Bergar Stamford, CT 06907-0047Laval (Quebec) H7L5A1Tel: (203) 359-1660Tel: (514) 856-6928FAX: (203)359-7700FAX: (514) 856-6886e-mail:**************e-mail:**************Servicing Europe:Benelux:Postbus 8034, 1180 LA Amstelveen,The Netherlands Tel: (31) 20 6418405 FAX: (31) 20 6434643Toll Free in Benelux: 06 0993344e-mail:************Czech Republic:ul. Rude armady 1868, 733 01 Karvina-Hranice, Czech Repubic Tel: 420 (69) 6311627 FAX: 420 (69)6311114e-mail:***************France:9, rue Denis Papin, 78190 Trappes Tel: (33) 130-621-400 FAX: (33)130-699-120Toll Free in France: 0800-4-06342e-mail:****************Germany/Austria:Daimlerstrasse 26, D-75392Deckenpfronn, Germany Tel: 49 (07056) 3017 FAX: 49 (07056) 8540TollFreeinGermany************e-mail:*****************United Kingdom: ISO 9002 Certified One Omega Drive Riverbend Technology Centre Northbank, Irlam,Manchester, M44 5EX, England Tel: 44 (161) 777-6611 FAX: 44 (161) 777-6622Toll Free in England: 0800-488-488e-mail:***************.ukINTRODUCTIONThis instrument is a portable 3½ digit, compact-sized digital Thermo-Hygrom-eter designed for simple one hand operation. Uses Platinum Resistance Tem-perature Detector Pt385/1000W (Alpha=0.00385) as temperature sensor, and uses thin film polymer capacitive type relative humidity sensor as hygrometer sensor.SAFETY INFORMATIONIt is recommended that you read the safety and operation instructions before using the hygrometer.CAUTION•Do not immerse the hygrometer sensor head into liquids since thiscauses permanent damage to the sensor.•The meter when not in use, please use protective metal cap cover thesensor head and rotate clockwise it to extend sensors life.The symbol on the instrument indicates that the operator must refer to an explanation in this manual.1SPECIFICATIONSGENERALDisplay: 3½ digit liquid crystal display (LCD) with maximum reading of 1999. Overtemperature: "OL" mark indication.Low battery indication: The "" is displayed when the battery voltage drops below the operating level.Measurement rate: 2.5 times per second, nominal.Accuracy: Stated accuracy at 23°C ± 5°C, <75% relative humidity. Temperature Coefficient: 0.1 times the applicable accuracy specification per °C from °C to 18°C and 28°C to 50°C.Operating environment: 0°C to 50°C at <75% relative humidity.Storage environment: -20°C to 60°C at <80% relative humidity.Battery: 4 pcs 1.5V (AAA size).Battery Life: 200 hours typical.Dimensions: 170mm(H) x 44mm(W) x 40mm(D).Weight: 160g (including probe and batteries).ELECTRICALTEMPERATURETemperature Scale: Celsius.Temperature Sensor: RTD Pt385/1000W.Measurement Range: -20°C to 100°C.Resolution: 0.1°C.Accuracy:±0.5°C0°C to 50°C±1°C -20°C to 0°C, 50°C to 100°CRELATIVE HUMIDITYHumidity Sensor: Electronic capacitance polymer film sensor. (The sensor is unaffected by water condensate, is immune to most reagent vapors) Measurement Range: 0% to 100%RH.Resolution: 0.1%RH.Accuracy:±2.5%RH (10% to 90%RH)±5%RH (<10%, > 90%RH)Sensor Response Time for 90% of Total Range: 60sec typical.Sensor Stability: ±2%RH, 2 years typical.Sensor Hysteresis (excursion of 10% to 90% to 10% RH): ±1%RH typical. Sensor Temperature Dependence: Negligible between 0°C to 50°C.OPERATING INSTRUCTIONSPush ButtonDisplay Back-Light ButtonPress "" button to toggle between turn on and turn off the Back-Light. DATA HOLD Buttonpresent readings and stops all further measurements.Press the DATA HOLD key again cancels DATA HOLD mode, causing hygrom-eter to resume taking measurements.MAX HOLD ButtonPress the MAX HOLD key to enter the MAX HOLD mode. The hygrometer then records and updates the maximum absolute values and the MAX annunciator appears on the display. Press the MAX HOLD key again to exit the MAX HOLD recording mode.In the MAX HOLD mode, press DATA HOLD key to stop the recording, press DATA HOLD key again to resume recording.OPERATION1. Rotate counter clockwise to remove the protective metal cap.2. Set the power switch to the desired %RH or °C range.3. Read the display.4. Cover sensor head to extend sensor life when not in use.SPECIAL CONSIDERATIONS•Before a reliable measurement can be made, the measuring hygrometer and medium to be measured must be in temperature and humidity eguilibrium.•Temperature measurement errorsDue to too short measurement time, sunshine during the measurement, heating, cold outer walls, air draft (e.g. fans), radiating hand and / or body heat etc.•Humidity measurement errorsDue to steam, water splashes, dripping water or condensation (not water condensate) on the sensor etc. However, repeatability and long-term stability are not impaired by this.•ContaminationBy dust in the air or measurements in powdery substances. This can be largely avoided by using a corresponding filter. The filter must be cleaned or replaced periodically depending upon the degree of contamination of the measuring site.OPERATOR MAINTENANCEBattery ReplacementPower is supplied by four 1.5V (AAA size) batteries. The "" appears on the LCD display when replacement is needed. To replace the batteries, remove the screw from the back of the meter and lift off the battery cover. Remove the batteries from battery contacts.CleaningPeriodically wipe the case with a damp cloth and detergent, do not use abrasives or solvents.WARRANTYOMEGA warrants this unit to be free of defects in materials and workmanship and to give satisfactory service for a period of 13 months from date of purchase. OMEGA Warranty adds an additional one (1) month grace period to the normal one (1) year product warranty to cover handling and shipping time. This ensures that OMEGA's customers receive maximum coverage on each product. If the unit should malfunction, it must be returned to the factory for evaluation. OMEGA's Customer Service Department will issue an Authorized Return (AR) number immediately upon phone or written request. Upon examination by OMEGA, if the unit is found to be defective it will be repaired or replaced at no charge. However, this WARRANTY is VOID if the unit shows evidence of having been tampered with or shows evidence of being damaged as a result of excessive corrosion; or current, heat moisture or vibration; improper specification; misapplication; misuse or other operating conditions outside of OMEGA's control. Components which wear or which are damaged by misuse are not warranted. This includes contact points, fuses, and triacs. OMEGA is glad to offer suggestions on the of use of its various products. Nevertheless, OMEGA only warrants that the parts manufactured by it will be as specified and free of defectsOMEGA MAKES NO OTHER WARRANTIES OR REPRESENTATIONS OF ANY KIND WHATSOEVER, EXPRESSED OR IMPLIED, EXCEPT THAT OF TITLE AND ALL IMPLIED WARRANTIES INCLUDING ANY WARRANTY OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE ARE HEREBY DISCLAIMED.LIMITATION OF LIABILITY: The remedies of purchaser set forth herein are exclusive and the total liability of OMEGA with respect to this order, whether based on contract warranty, negligence, indemnification, strict liability or otherwise, shall not exceed the purchase price of the component upon which liability is based. In no event shall OMEGA be liable for consequential, incidental or special damages.Every precaution for accuracy has been taken in the preparation of this manual; however, OMEGA ENGINEERING, INC. neither assumes responsibility for any omissions or errors that may appear nor assumes liability for any damages that result from the use of the products in accordance with the information contained in the manual.SPECIAL CONDITION: Should this equipment be used in or with any nuclear installation or activity, purchaser will indemnity OMEGA and hold OMEGA harmless from any liability or damage whatsoever arising out of the use of theIt is the policy of OMEGA to comply with all worldwide safety and EMC/EMI regulations that apply. OMEGA is constantly pursuing certification of its products to the European New Approach Directives. OMEGA will add the CE mark to every appropriate device upon certification.The information contained in this document is believed to be correct but OMEGA Engineering, Inc. accepts no liability for any errors it contains, and reserves the right to alter specifications without notice.WARNING: These products are not designed for use in, and should not be used for, patient connected application.RETURN REQUESTS / INQUIRIESDirect all warranty and repair requests/inquiries to the OMEGA Customer Service Department. BEFORE RETURNING ANY PRODUCT(S) TO OMEGA, PURCHASER MUST OBTAIN AN AUTHORIZED RETURN (AR) NUMBER FROM OMEGA'S CUSTOMER SERVICE DEP ARTMENT (IN ORDER TO AVOID PROCESSING DELAYS). The assigned AR number should then be marked on the outside of the return package and on any correspondence.The purchaser is responsible for shipping charges, freight, insurance and proper packaging to prevent breakage in transit.FOR WARRANTY RETURNS, please have the following information available BEFORE contacting OMEGA: 1.P.O. number under which the product wasPURCHASED.2.Model and serial number of the product underwarranty, and3.Repair instructions and/or specific problems relativeto the product.FOR NON-WARRANTY REPAIRS, consult OMEGA for current repair charges. Have the following information available BEFORE contacting OMEGA:1.P.O. number to cover the COST of the repair.2.Model and serial number of product , and3.Repair instructions and/or specific problems relativeto the product.OMEGA's policy is to make running changes, not model changes, whenever an improvement is possible. This affords our customers the latest in technology and engineering. OMEGA is a registered trademark of OMEGA ENGINEERING, INC. © Copyright 1999 OMEGA ENGINEERING, INC. All rights reserved. This document may not be copied, photocopied, reproduced, translated, or reduced to any electronic medium or machine-readable from, in whole or in part, without prior written consent of OMEGA ENGINEERING, INC.Where Do I Find Everything I Need for Process Measurement and Control?OMEGA...Of Course!HEATERSþHeating CableþCartridge & Strip HeatersþImmersion & Band HeatersþFlexible HeatersþLaboratory HeatersPRESSURE/STRAIN AND FORCE þTransducers & Strain GaugesþLoad Cells & Pressure GaugesþDisplacement TransducersþInstrumentation & AccessoriesFLOW/LEVELþRotameters, Gas MassFlowmeters & Flow Computers þAir Velocity IndicatorsþTurbine/Paddlewheel SystemsþTotalizers & Batch Controllers TEMPERATUREþThermocouple, RTD & ThermistorProbes, Connectors, Panels &AssembliesþWire: Thermocouple, RTD &ThermistorþCalibrators & Ice Point ReferencesþRecorders, Controllers & ProcessMonitorsþInfrared PyrometersENVIRONMENTALMONITORING AND CONTROLþMetering & Control InstrumentationþRefractometersþPumps & TubingþAir, Soil & Water MonitorsþIndustrial Water & WastewaterTreatmentþpH, Conductivity & DissolvedOxygen InstrumentspH/CONDUCTIVITYþpH Electrodes, Testers &AccessoriesþBenchtop/Laboratory MetersþControllers, Calibrators,Simulators & PumpsþIndustrial pH & ConductivityEquipmentDATA ACQUISITIONþData Acquisition & EngineeringSoftwareþCommunications-BasedAcquisition SystemsþPlug-in Cards for Apple, IBM& CompatiblesþDatalogging SystemsþRecorders, Printers & PlottersM-2860/0799。

CS3W-435|440|445|450|455|460MSMORE POWER435 W ~ 460 W* For detailed information, please refer to Installation Manual.CSI Solar Co., Ltd. is committed to providing high quality solar products, solar system solutions and services to customers around the world. Canadian Solar was recognized as the No. 1 module supplier for quality and performance/price ratio in the IHS Module Customer Insight Survey, and is a leading PV project developer and manufacturer of solar modules, with over 50 GW deployed around the world since 2001.HiKuHIGH POWER MONO PERC MODULE26 % more power than conventional modules Low NMOT: 42 ± 3 °CLow temperature coefficient (Pmax): -0.35 % / °CMORE RELIABLEUp to 4.5 % lower LCOEUp to 2.7 % lower system cost Heavy snow load up to 5400 Pa, wind load up to 3600 Pa*42°CBetter shading toleranceLower internal current, lower hot spot temperatureMinimizes micro-crack impactsIEC 61215 / IEC 61730 / CE / MCS / INMETROFSEC (US Florida) / UL 61730 / IEC 61701 / IEC 62716 UNI 9177 Reaction to Fire: Class 1 / Take-e-wayPRODUCT CERTIFICATES*ISO 9001:2015 / Quality management systemISO 14001:2015 / Standards for environmental management systemISO 45001: 2018 / International standards for occupational health & safetyMANAGEMENT SYSTEM CERTIFICATES**According to the applicable Canadian Solar Limited Warranty Statement.12YearsYears1st year power degradation no more than 2%Subsequent annual power degradation no more than 0.55%Enhanced Product Warranty on Materialsand Workmanship*Linear Power Performance Warranty*CSI Solar Co., Ltd.199LushanRoad,SND,Suzhou,Jiangsu,China,215129,,********************* The specific certificates applicable to different module types and markets will vary, and therefore not all of the certifications listed herein will simultaneously apply to the products you order or use. Please contact your local Canadian Solar sales representative to confirm the specific certificates available for your product and applicable in the regionsin which the products will be used.PARTNER SECTIONELECTRICAL DATA | STC*CS3W435MS 440MS 445MS 450MS 455MS 460MSNominal Max. Power (Pmax)435 W 440 W 445 W 450 W 455 W 460 W Opt. Operating Voltage (Vmp)40.5 V 40.7 V 40.9 V 41.1 V 41.3 V 41.5 V Opt. Operating Current (Imp)10.75 A 10.82 A 10.89 A 10.96 A 11.02 A 11.09 A Open Circuit Voltage (Voc)48.5 V 48.7 V 48.9 V 49.1 V 49.3 V 49.5 V Short Circuit Current (Isc)11.42 A 11.48 A 11.54 A 11.60 A 11.66 A 11.72 A Module Efficiency 19.7%19.9%20.1%20.4%20.6%20.8%Operating Temperature -40°C ~ +85°CMax. System Voltage 1500V (IEC/UL) or 1000V (IEC/UL)Module Fire Performance TYPE 1 (UL 61730 1500V) or TYPE 2 (UL 61730 1000V) or CLASS C (IEC 61730)Max. Series Fuse Rating 20 A Application Classification Class A Power Tolerance* Under Standard Test Conditions (STC) of irradiance of 1000 W/m 2, spectrum AM 1.5 and cell tempera-ture of 25°C.ELECTRICAL DATA | NMOT*CS3W435MS 440MS 445MS 450MS 455MS 460MSNominal Max. Power (Pmax)325 W 328 W 332 W 336 W 339 W 343 W Opt. Operating Voltage (Vmp)37.8 V 37.9 V 38.1 V 38.3 V 38.5 V 38.7 V Opt. Operating Current (Imp)8.59 A 8.65 A 8.71 A 8.76 A 8.82 A 8.87 A Open Circuit Voltage (Voc)45.6 V 45.8 V 46.0 V 46.2 V 46.4 V 46.6 V Short Circuit Current (Isc)9.21 A 9.26 A 9.31 A 9.36 A 9.41 A 9.45 A* Under Nominal Module Operating Temperature (NMOT), irradiance of 800 W/m 2, spectrum AM 1.5, ambient temperature 20°C, wind speed 1 m/s.CS3W-435MS / I-V CURVESMECHANICAL DATA Specification DataCell TypeMono-crystalline Cell Arrangement 144 [2 X (12 X 6) ]Dimensions 2108 X 1048 X 35 mm (83.0 X 41.3 X 1.38 in)Weight 24.3 kg (53.6 lbs)Front Cover 3.2 mm tempered glass Frame Anodized aluminium alloy J-BoxIP68, 3 bypass diodesCable4 mm 2 (IEC), 12 AWG (UL)Cable Length(Including Connector)500 mm (19.7 in) (+) / 350 mm (13.8 in) (-) or customized length*Connector T4 series or H4 UTX or MC4-EVO2 Per Pallet30 piecesPer Container (40' HQ)660 pieces* For detailed information, please contact your local Canadian Solar sales and technical representatives.VA 1211109876543210 5 10 15 20 25 30 35 40 45 50 V A5 10 15 20 25 30 35 40 45 501000 W/m 800 W/m600 W/m 400 W/m200 W/m5°C 25°C 45°C 65°CTEMPERATURE CHARACTERISTICS SpecificationData Temperature Coefficient (Pmax)-0.35 % / °C Temperature Coefficient (Voc)-0.27 % / °C Temperature Coefficient (Isc)0.05 % / °CNominal Module Operating Temperature 42 ± 3°C1211109876543210 ~ + 10 WFeb. 2021. All rights reserved, PV Module Product Datasheet V5.61_ENCSI Solar Co., Ltd.199LushanRoad,SND,Suzhou,Jiangsu,China,215129,,********************* The specifications and key features contained in this datasheet may deviate slightly from our actual products due to the on-going innovation and product enhancement .CSI Solar Co., Ltd. reserves the right to make necessary adjustment to the information described herein at any time without further notice.Please be kindly advised that PV modules should be handled and installed by qualified people who have professional skills and please carefully read the safety and installation instructions before using our PV modules.ENGINEERING DRAWING (mm)Rear ViewMounting HoleA-AB-BFrame Cross Section。

Luc-Pair ™ Duo-Luciferase Assay Kit 2.0For luciferase assaysCat. No. LF001 (Old Cat. No. LPFR-P010, 100 reactions) Cat. No. LF002 (Old Cat. No. LPFR-P030, 300 reactions) Cat. No. LF003 (Old Cat. No. LPFR-P100, 1000 reactions)User ManualGeneCopoeia, Inc.9620 Medical Center Drive, #101 Rockville, MD 20850 USA301-762-0888 866-360-9531***********************© 2017 GeneCopoeia, Inc.Expressway to Discovery G C n p o eiae e o TMUSER MANUALLuc-Pair™ Duo-Luciferase Assay Kit 2.0I. Introduction and PrinciplesII. Contents and StorageIII. Preparation of Cell Lysates Using Lysis BufferIV. Preparation of FLuc and RLuc Assay Working SolutionV. ProcedureVI. Limited Use License and WarrantyI. Introduction and PrinciplesThe study of transcriptional regulation using reporter gene expression is common and necessary in cell biology research and pharmaceutical discovery. Luciferase is the most widely used genetic reporter for gene expression studies due to several advantages, including:1) high sensitivity in a large dynamic range2) natural absence from mammalian cells3) consistent reproducibility4) cost effectiveness5) simple assay format6) suitable for high-throughput screeningFirefly and Renilla luciferases have been widely used as co-reporters for normalization studies because both assays are quick, easy and sensitive. Firefly and Renilla luciferases are ideal co-reporters because they have distinct evolutionary origins and very different enzyme structures and substrates. This renders cross-reactivity obsolete. Firefly (Photinus pyralis) luciferase has been proven to be an ideal reporter for monitoring both promoter activity and post-transcriptional regulation in the control of gene expression. It is a cytoplasmic enzyme with a molecular weight of about 61 kDa and catalyzes the following reaction:The intensity of light emission is proportional to the amount of luciferase and is measured using a luminometer and luminescence plate reader.Renilla (Renilla reniformis) luciferase is a 36 kDa monomeric protein, which requires no post-translational processing. Therefore, it can function as a real-time transcription reporter. It catalyzes the following reaction:Using this assay system allows one to monitor the transcriptional activation of cis-elements in proximity to the gene of interest. However, it has been more difficult to measure the transcriptional repression via 3’ UTR regulation of genes since the enzyme-substrate activity window is relatively small. Longer stability of the enzyme-substrate complex allows greater flexibility in monitoring true repressive events. Further, biological variation and stochastic events may add noise, thereby reducing the differences in observed luciferase activity. Thus, normalizing the expression of an experimental reporter to the expression of an independent control reporter can help differentiate between true signal and nonspecific cellular responses. Normalization is also needed for adjusting differences in transfection efficiencies and cell viability.GeneCopoeia has leveraged the differences in Firefly and Renilla enzyme structures and substrates to optimize and develop a convenient system for measuring two luciferase activities in succession. The assay measures the activities of Firefly and Renilla luciferases sequentially from a single sample. The Firefly luciferase luminescence is produced by one reagent, while a second reagent simultaneously quenches the Firefly luciferase and produces Renilla luciferase luminescence.The GeneCopoeia Luc-Pair TM Duo-Luciferase Assay Kit 2.0 development team incorporated several features into the reagents to enhance product performance and convenience, including the following:∙Enhanced stability. The reagents have been developed so that the signals for firefly and Renilla luciferases exhibit greater stability than the original Luc-Pair TM reagents and competitor kits (Figure 1). 30 minutes after addition of the appropriate reagents at 22-25°C, firefly luciferase activity will be at least 80% of its initial activity, while Renilla luciferase activity will be at least 95% of its initial activity.∙Versatility. The system has been designed for use with many different eukaryotic (vertebrates, lower invertebrates) cells lines and is optimized for use on micro-plate readers for high-throughput screening assays. Single-tube samples can also be used.∙Low background. The system produces very limited background luminescence. No subtraction is required from readings.∙Simplicity. Renilla luciferase buffer and substrate contains the quencher firefly luciferase activity. This allows for a quick two-step assay (Figure 2).∙Reproducibility. This system is designed to yield reliable, linear results for a concentration range over several orders of magnitude (Figure 3).Figure 1. Stability of Firefly luciferase and Renilla luciferase signals using GeneCopoeia (GCI) Luc-Pair™Duo-Luciferase Assay Kit 2.0. HEK 293 cells were transfected with GeneCopoeia pEZX-MT06 miRNA reporter vector for 48 hours. A.) The FLuc and RLuc activity was measured as described in the procedure. B.) The Competitor’s dual-luciferase assay kit was used in comparison.Figure 2. Firefly luciferase activity is quenched with the addition of Buffer II. HEK 293 cells were transfected with GeneCopoeia pEZX-MT06 miRNA reporter vector for 48 hours. The Firefly luciferase activity was measured as described in the procedure. Afterwards, 1x Buffer II (without substrate) was added to the wells, followed by count reading in a luminometer.Figure 3. Linear relationship between emitted light and amount of luciferase expression vector transfected in HEK293. HEK 293 cells were transfected with GeneCopoeia pEZX-MT06 miRNA reporter vector for 48 hours. The FLuc (A) and RLuc (B) activities were measured in lysates from different numbers of cells after incubation for 3 min as described in the procedure.II.Contents and StorageThe Luc-Pair™ Duo-Luciferase Assay Kit 2.0 (Cat.Nos. LF001, LF002, LF003)III. Preparation of Cell Lysates Using Lysis BufferThe Lysis Buffer is supplied as a 10×concentrate. It may show turbid after thawing and mix which won’t affect the luciferase assays. Vortex 3-5 sec after thawing, and prepare a sufficient quantity of the 1×working concentration by adding 1 volume of 10×Lysis Buffer to 9 volumes of distilled water and mix. The diluted (1×) Lysis Buffer may be stored at -20°C for 1-2 months; however, we recommend preparing the volume of Lysis Buffer required just before use.A. Lysis of Cells Cultured in Multi-well Plates1. Determine transfection parameters (i.e., plated cell density and subsequent incubation time) such that cells are 80-95%confluent at the desired time of lysate preparation. Remove the growth medium from the cultured cells, and gently applya sufficient volume of phosphate buffered saline (PBS) to wash the surface of the culture vessel. Swirl the vessel brieflyto remove detached cells and residual growth medium. Completely remove the rinse solution before applying LysisBuffer.2. Dispense into each culture well the minimum volume of 1×Lysis Buffer required to completely cover the cell monolayer.The recommended volumes of 1×Lysis Buffer to add per well are as follows:Note: The Lysis Buffer provided in the kit is sufficient for directly lysing cells in 24-, 48- or 96-well culture plates. If a 6-well or 12-well plates are used, we recommend either purchasing more Lysis Buffer: Luc-Lysis buffer(10×), Cat. No.LF003-01, or harvesting cells by scraping or trypsinization according to the procedures in III-B below.3. Place the culture plates on a rocking platform or orbital shaker with gentle rocking/shaking to ensure complete and evencoverage of the cell monolayer with 1×Lysis Buffer. Rock the culture plates at room temperature for 10-15 minutes.Note1: If cell clumps appear, pipetting up and down several times could be helpful to disperse the cells. Overgrown cells are more resistant to complete lysis, and typically require an increased volume of lysis buffer or extra steps toensure complete lysis (See Note 2).Note2: If the cells are hard to lyse on the plates after adding Lysis Buffer, there are several steps you can take to help lyse the cells:Use a cell scraper to scrape and collect the cells into tubes, freeze-thaw several times, and vortex to help lyse the cells.Freeze-thaw the culture plates several times and pipette up and down several times to help detach and lyse the cells.Add more lysis buffer, and pipette up and down to help detach and lyse the cells.Note3:The Firefly and Renilla luciferases contained in the cell lysates are stable for at least 30 minutes at room temperature (22°C) and up to 2 hours on ice. -70°C is recommended for long-term storage. Subjecting cell lysates tomore than 5 freeze-thaw cycles may result in gradual loss of luciferase reporter enzyme activities.4. Transfer the lysate to a tube or vial for further handling and storage. Alternatively, reporter assays may be performeddirectly in the 96-well culture plate if the plates are compatible with the type of luminometer being used.B. Lysis of Cells in tubes.5. For cells cultured in suspension, or cells harvested by scraping or trypsinization. Collect 1-2×105 cells in 1.5mL tubes,rinse cells with 1mL of PBS buffer, spin at 500g for 5 minutes, and completely remove the rinse solution.6. Add 50-100μl of 1×Lysis Buffer to make 2×103 cells/μl, Vortex 5-10 sec to completely disperse the cells, then 1 to 2freeze-thaw cycles to accomplish complete lysis of cells.7. Proceed to Luciferase assay.Note:The Firefly and Renilla luciferases contained in the cell lysates are stable for at least 30 minutes at room temperature (22°C) and up to 2 hours on ice. -70°C is recommended for long-term storage. Subjecting cell lysates tomore than 5 freeze-thaw cycles may result in gradual loss of luciferase reporter enzyme activities. 2×103cells/μL in1×Lysis Buffer is good for the assay in normal transfected cells. If the cells have lower transfection efficiency or thepromoter is very weak, you may increase the cell numbers. This Lysis Buffer is optimized for compatible with thefollowing FLuc and RLuc detection assays. If other cell lysis buffers are used, the signal strength and stability of theluciferases could be affected.IV. Preparation of FLuc and RLuc Assay Working SolutionNote 1. Luc Buffers I and II are stable at -20°C for at least 6 months. Freezing and thawing the reagents 3-4 cycles won’t affect the activity of the luciferases. Aliquotting is recommended if more freeze-thaw cycles are required.Note 2. Working Solutions (Buffers contain Substrates) are stable at room temperature for 1-2 hours. Prepare only the required amount of Working Solution before use.Note 3. Light intensity is a measure of the rate of catalysis by the luciferases, and is therefore, temperature sensitive. The temperature optimum for the activity of both luciferases is approximately room temperature (20-25°C), so it is important that the reagents be equilibrated to room temperature before beginning measurements. This kit is designed for dual luciferase detection. If using for detection of single luciferase, the procedures for dual luciferase detection in this manual should still be strictly followed.1. Thaw Luc Buffer I (5×) and Luc Buffer II (5×) thoroughly at room temperature, inverting the tube several times and thenvortexing for 3-5 seconds.Note: Some pellets may appear in the Luc Buffer II (5×) after thawing. It is important to completely dissolve the pellets before using. Incubation at 37°C for 5-10 minutes and more vortexing will be necessary to fully re-dissolve the pellets.2. Dilute 1:5 in distilled water to make 1×Luc Buffer I and 1×Luc Buffer II. Prepare 100µL of each Buffer for each reaction(well). Duplicates or triplicates for each sample are recommended.Example: If you have 5 samples in duplicated reactions, prepare 1mL of 1×Luc Buffer I and 1×Luc Buffer II. By diluting0.2mL of each 5×Buffers with 0.8mL ddH2O respectively. Preparing a little extra may be helpful to avoid buffer shortagecaused by pipetting error.3. Prepare the FLuc and RLuc Assay Working Solution (e.g.10 samples) by adding 10μL of Luc Sub I and II (100×) to 1mLof 1×Luc Buffer I and 1×Luc Buffer II, respectively. Mix well by inverting the tube several times.4. Incubate at room temperature for 5 minutes (capped and protected from light) before adding to the samples.Note: The RLuc Assay Working Solution will be used after reading the FLuc assay. It can be kept at room temperature as long as 1 hour if properly capped and protected from light.V. Assay Procedure1. Set up the luminometer. Follow the manual associated with your plate reader. Set the measurement for 1-2 seconds ofintegration.2. Pipette the cell lysis samples (20μL per well) into a 96-well white (opaque) or black plate, or luminometer tubes.3. Add the FLuc Assay Working Solution from step IV-4 (100μL per well or tube) to the samples. Gently pipette up anddown mix the sample and assay solution. Do not vortex.Note:If you have many samples and use 96-well plates, we recommend using a multi-channel pipette in order to reduce the time between additions of Assay Working Solution to each well.Auto-Injector: is not recommended for this kit.4. Incubate at room temperature for 3-5 minutes and proceed with the measurement.Note:Read the plate(s) within 5 minutes after the incubation. If using single luminometer tubes, make sure the incubation and processing times before the luminescence detection are identical for all samples.5. Save the reading if the luminometer reader does not automatically record the readings.6. Remove the plates or luminometer tubes.7. Add the RLuc Working Solution from Step IV-4 (100μL per well or tube) to the plates or tubes from Step V-6. Gentlypipette up and down or tap the plate (tube) several times to mix the sample and assay solution. Do not vortex.Note:If you have many samples and use 96-well plates, we recommend using a multi-channel pipette in order to reduce the time between additions of Assay Working Solution to each well.Auto-Injector: is not recommended for this kit.8. Incubate at room temperature for 3-5 minutes and proceed with the measurement.Note:Read the plate(s) within 5 minutes after the incubation. If using single luminometer tubes, make sure the incubation and processing times before the luminescence detection are identical for all samples.9. Record the reading if the luminometer reader does not automatically save the readings.10. Remove the plates or luminometer tubes.11. Calculate the ratio of luminescence from the Firefly luciferase to the Renilla luciferase.IMPORTANT NOTE:Because the luminescent signals are affected by assay conditions, raw results should be compared only between samples measured at the same time and using the same medium/serum combination.Incorporation of consistent control wells on each plate provides the ability to calculate a normalized Fireflyluminescence/Renilla luminescence ratio for each sample well. These normalized ratios will remain essentially constant(±10%) for samples in a plate measured during the 1-hour measurement window. This kit is designed for dual luciferasedetection. If using for detection of single luciferase, the procedure for dual luciferase detection in this manual still shouldbe strictly followed. You may also purchase our single luciferase detection kits.VII. Limited Use License and WarrantyLimited Use LicenseFollowing terms and conditions apply to use of the Luc-Pair™ Duo-Luciferase Assay Kit 2.0 (the Product). If the terms and conditions are not acceptable, the Product in its entirety must be returned to GeneCopoeia within 5 calendar days. A limited End-User license is granted to the purchaser of the Product. The Product shall be used by the purchaser for internal research purposes only. The Product is expressly not designed, intended, or warranted for use in humans or for therapeutic or diagnostic use. The Product must not be resold, repackaged or modified for resale, or used to manufacture commercial products or deliver information obtained in service without prior written consent from GeneCopoeia. This Product should be used in accordance with the NIH guidelines developed for recombinant DNA and genetic research. Use of any part of the Product constitutes acceptance of the above terms.Limited WarrantyGeneCopoeia warrants that the Product meets the specifications described in the accompanying Product Datasheet. If it is proven to the satisfaction of GeneCopoeia that the Product fails to meet these specifications, GeneCopoeia will replace the Product. In the event a replacement cannot be provided, GeneCopoeia will provide the purchaser with a refund. This limited warranty shall not extend to anyone other than the original purchaser of the Product. Notice of nonconforming products must be made to GeneCopoeia within 30 days of receipt of the Product. GeneCopoeia’s liability is expressly limited to replacement of Product or a refund limited to the actual purchase price. GeneCopoeia’s liability does not extend to any damages arising from use or improper use of the Product, or losses associated with the use of additional materials or reagents. This limited warranty is the sole and exclusive warranty. GeneCopoeia does not provide any other warranties of any kind, expressed or implied, including the merchantability or fitness of the Product for a particular purpose.GeneCopoeia is committed to providing our customers with high-quality products. If you should have any questions or concerns about any GeneCopoeia products, please contact us at 301-762-0888.© 2017 GeneCopoeia, Inc.。

梅特勒Easymax量热仪是一种广泛应用于化学、生物、药学等领域的热学测试仪器。

它能够精确测量化学反应的热变化，为科研工作者提供了重要的实验数据。

在使用Easymax量热仪之前，了解它的原理对于正确操作和解读实验结果非常重要。

本文将介绍梅特勒Easymax量热的原理。

一、热量的测量原理梅特勒Easymax量热仪的原理是基于热量的测量。

在化学反应中，原料转化为产物释放或吸收热量，这种热量变化可以直接反映出反应的特性。

Easymax量热仪使用高灵敏度的热敏传感器，能够准确地捕捉这种热量变化，从而实现实验数据的准确测量。

二、热量的传递原理在Easymax量热仪中，样品和试剂通过特殊设计的反应池进行反应，而热量则通过传热系统传递到热敏传感器上。

传热系统采用高效的热导材料，能够快速而均匀地将反应中释放或吸收的热量传递给热敏传感器，确保实验数据的准确性。

三、实验数据的处理原理Easymax量热仪通过高精度的数据采集系统，能够实时记录反应过程中的温度变化并将其转化为热量变化数据。

这些数据经过内置的计算模型处理后，可以得到反应的热力学参数，如反应热、反应速率等，为科研工作者提供了重要的参考信息。

四、优化原理梅特勒Easymax量热仪在设计上充分考虑了操作便捷性和实验效率。

其智能化的操作界面和丰富的实验模式，能够满足不同领域、不同需求的科研工作者的实验要求。

Easymax量热仪的模块化设计使得其维护和升级更加方便快捷，为用户提供了持久而稳定的实验评台。

总结：梅特勒Easymax量热仪基于热量测量原理，通过传热系统和数据处理原理实现了对化学反应热量变化的精确测量。

其优化的设计理念使其成为广泛应用于科研领域的重要实验工具。

了解Easymax量热仪的原理，有助于科研工作者正确操作和解读实验数据，推动科研工作的顺利进行。

梅特勒Easymax量热仪的原理和优势让它成为科研工作者的得力助手，下面我们将进一步深入了解其原理，并探讨其在实验中的应用。

Product InformationKrytox™ oils and greases are based on perfluoropolyether (PFPE) oils. Chemically inert and safe for use aroundmost chemicals and reactive gases, these lubricants are nonflammable and are also safe for use in oxygen service. Krytox™ oils and greases are compatible with commonly used materials and do not damage plastics or elastomers, nor cause corrosion to metals.Krytox™ oils and greases are commonly used as lubricantsin aerospace, automotive, industrial, and semiconductor applications, as well as in solving many other routine lubrication problems. In addition, they provide exceptionally long lifetimes in sealed-for-life bearings and can extendre-lubrication intervals in bearings that require re-lubrication. Krytox™ base oil is a clear, colorless, fluorinated synthetic oil; it is a PFPE, but is also called perfluoroalkylether (PFAE) or perfluoropolyalkylether (PFPAE) with the following chemical structure:F-(CF-CF2-O)n-CF2CF3| where n=10–60CF3The polymer chain is completely saturated and contains only carbon, oxygen, and fluorine. On a weight basis, a typical Krytox™oil contains 21.6% carbon, 9.4% oxygen, and 69.0% fluorine. Krytox™ GPL 203 LubricantKrytox™ GPL 203 grease is polytetrafluoroethylene (PTFE)-thickened, contains no additives, and can be used on components that come in contact with chemicals. Typical applications include valves, instruments, or bearings that come into contact with chemicals, such as alcohols, ammonia, solvents, steam, acids and bases, and oxygen systems, e.g., LOX1 and GOX2. Krytox™ GPL 203 grease is commonly used as a lubricant for seals and O-rings, and is compatible with most types of seals.Krytox™ oils and greases are silicone-free. They do not contain any VOC3 materials or chlorine and are not hazardous tothe atmosphere or ozone layer. They are biologically and environmentally inert.1LOX = Liquid oxygen2GOX = Gaseous oxygen3VOC =Volatile organic compounds© 2015 The Chemours Company FC, LLC. Krytox ™ and any associated logos are trademarks or copyrights of The Chemours Company FC, LLC. Chemours ™ and the Chemours Logo are trademarks of The Chemours Company.Replaces: K-23315-2C-10350 (10/15)Krytox ™Performance LubricantsThe information set forth herein is furnished free of charge and based on technical data that Chemours believes to be reliable. It is intended for use by persons having technical skill, at their own discretion and risk. The handling precaution information contained herein is given with the understanding that those using it will satisfy themselves that their particular conditions of use present no health or safety hazards. Because conditions of product use are outside our control, Chemours makes no warranties, express or implied, and assumes no liability in connection with any use of this information. As with any material, evaluation of any compound under end-use conditions prior to specification is essential. Nothing herein is to be taken as a license to operate under or a recommendation to infringe any patents.NO PART OF THIS MATERIAL MAY BE REPRODUCED, STORED IN A RETRIEVAL SYSTEM OR TRANSMITTED IN ANY FORM OR BY ANY MEANS ELECTRONIC, MECHANICAL, PHOTOCOPYING, RECORDING OR OTHERWISE WITHOUT THE PRIOR WRITTEN PERMISSION OF CHEMOURS.For product information, industry applications, technical assistance, or global distributor contacts, visit or within the U.S. and Canada, call 1-844-773-CHEM/2436 or outside of the U.S., call 1-302-773-1000.The high-temperature stability of fully fluorinated Krytox ™ lubricants improves bottom-line savings from improved reliability as well as reduced grease usage and manpower through extended re-lubrication intervals. Excellent film strength reduces wear to reduce maintenance costs. Under high loads, the viscosity increases to provide support and absorb pressure.Typical ApplicationsApplications for these lubricants are generally of a critical nature. T emperatures in all industries are reaching extremes for conventional lubricants; lubricants are expected to be durable in the most aggressive environments and are now often considered an integral part of the design. Where extreme conditions and extreme performance demands prevail, whether because of durability, warranty, safety, loss of productivity, or downtime, Krytox ™ lubricants are the ideal choice in a wide range of industries and applications.Preparing the BearingsNew, unlubricated bearings often have rust-preventive oils in them to prevent damage while they are in storage prior to use. Before using new bearings, they should be inspected for damage and cleanliness*. When preparing to use Krytox ™ lubricant, greases or preservative oils need to be removed.Failure to do so could result in reduced bearing life. Bearing life tests on uncleaned bearings have shown reduced life in high temperature, high-speed tests where the bearing was filled with a minimum amount of grease. The preservatives coat the metal surface to prevent rusting; therefore, they can also prevent the grease from adhering, causing them to be thrown off by the action of the bearing. The preservatives could also oxidize and harden, and create debris that will contaminate the new grease.Storage and Shelf LifeBecause of the inert, non-oxidizing nature of the ingredients, Krytox ™ lubricants have an indefinite shelf life if unopened and stored in a clean dry location. Greases might show oil separation after extended storage, but mixing the free oil back into the grease will return the grease to its normal usable condition.*For guidelines on bearing preparation, ask for literature item no. K-22116-1, ”New Bearing Preparation.”。

e-mail:**************For latest product manuals:Shop online at SMUser’s GuideTSD6002The information contained in this document is believed to be correct, but OMEGA accepts no liability for any errors it contains, and reserves the right to alter specifications without notice.Servicing North America:U.S.A.:Omega Engineering, Inc., One Omega Drive, P.O. Box 4047S tamford, CT 06907-0047 USAToll-Free: 1-800-826-6342 (USA & Canada only)Customer Service: 1-800-622-2378 (USA & Canada only) Engineering Service: 1-800-872-9436 (USA & Canada only) Tel: (203) 359-1660 Fax: (203) 359-7700 e-mail:**************For Other Locations Visit /worldwide***********************The TSD600 is a temperature indicator, data logger, alarm unit and configuration tool for Omega infrared temperature sensors. It is compatible with all models in the OS210-C4 and OS-MINIHUB series, as well as all OS-MINI and OS-MINIFB models with Modbus output.The TSD600 functions as the Modbus Master on an RS485 network of up to 6 temperature sensors, and can itself be connected as a slave device to another RS485 network via a second, isolated Modbus interface. This allows multiple TSD600 units to be multi-dropped to create a large network of sensors and displays.Optional alarm relay modules allow the TSD600 to be connected to alarm equipment such as sounders and beacons, and optional analogue output modules allow it to be connected to non-Modbus instrumentation.All the configurable parameters for the hub, the connected sensors and the optional output modules are adjustable via the TSD600’s built-in resistive touch screen interface, which can be operated even with gloves on.With an optional MicroSD Card inserted, the TSD600 functions as a fully-configurable data logger. SPECIFICATIONSDisplay 2.83” (72 mm) resistive touch TFT,320 x 240 pixels, backlitSupply Voltage 10 to 30 V DCMaximum Current Draw 100 mAAmbient Temperature Range 0°C to 60°CRelative Humidity Maximum 95%, non-condensing Configurable Parameters (global) Temperature units, date and time, data logging,graph channels, alarm loggingConfigurable Parameters (per channel) Signal processing, emissivity setting, reflected energycompensation, alarms, Modbus addressAlarm Configuration12 alarms (2 per sensor) with adjustable level,individually configurable as HI or LO. Temperature Units °C or °F selectableTemperature Resolution 0.1° below 1000°; 1° above 1000°Signal Processing Average, peak hold, valley hold, minimum, maximum Display Sample Period120 ms per device (720 ms in total for 6 devices) Compatible Sensor Types OS201-C4 (all models),OS-MINIHUB (all models),OS-MINI (-BB and -BRT models),OS-MINIFB (–BRT models)Compatible Output Module Types ICP DAS M-7061 12-channel relay output moduleICP DAS M-7024 4-channel analogue outputmodule, selectable V/mAMECHANICALDimensions98(w) x 64(h) x 36(d) mm excluding cable glands Weight280 g1DATA LOGGINGWith an optional MicroSD Card installed in the slot inside the TSD600, data logging may be manually started and stopped via a button on the temperature display screen, or scheduled to begin at a pre-determined time via the Settings menu.If the TSD600 is connected via the slave interface to another Modbus network, logging may also be started remotely by the Modbus Master on that network.DATA LOGGING SPECIFICATIONSLogging Interval 1 to 86,400 seconds (1 day)MicroSD Card Max. capacity: 32 GB (not included)Internal Clock Battery 1 x BR 1225 3V (not included)Variables Logged T arget temperature, sensing head temperature, alarm events File Format.csv (can be imported to Excel)Configurable Parameters Sample period, number of samples, scheduled start dateand time2The TSD600 has removable screw terminal blocks for the Modbus Slave and Modbus Master interfaces.− Connect the Master interface to the sensors and output modules. Be sure to check the power supply requirements of each device before applying power.− Optionally, connect the Slave interface to another Modbus network with its own Modbus Master such as a PLC or SCADA system.Isolation is provided between the Slave and Master interfaces.MICROSD CARD AND BATTERYThe MicroSD Card and battery slots are located on the touch screen circuit board. Unscrew the lid of the TSD600 to access them.The battery is optional. With a battery fitted, the internal clock will continue to run when the power is off. Without a battery, the unit will request the date and time each time the power is cycled.All other settings are stored in the unit’s permanent memory and will be preserved when it isswitched off, regardless of whether a battery is fitted.3PASSWORDThe default password is 1234. The password may be changed via the touch screen interface.USING THE TOUCH SCREEN INTERFACEVisit for help on how to use the touch screen interface, including a diagramshowing how to navigate to each setting.4MODBUS OVER SERIAL LINE (RS485)INTERFACEBaud rate9600Format8 data, No parity, 1 stop bitReply delay (ms) 20SUPPORTED FUNCTIONSRead register0x03, 0x04Write single register 0x06Write multiple register 0x10The list below includes all available addresses:R = Read, W = Write5SENSOR SETTINGS ADDRESS SPACEThe settings of attached sensors can be read by adding the following offsets to the addresses speci-fied by the sensor manufacturer:See sensor manual for further details.Notes:1. For further information please refer to /specs.php2. Use address 255 to communicate with any connected unit (only one sensor connected)3. Use address 0 to broadcast to all connected units (no response expected)6WARRANTY/DISCLAIMEROMEGA ENGINEERING, INC. warrants this unit to be free of defects in materials and workmanship for a period of 13 months from date of purchase. OMEGA’s WARRANTY adds an additional one (1) month grace period to the normal one (1) year product warranty to cover handling and shipping time. This ensures that OMEGA’s customers receive maximum coverage on each product.If the unit malfunctions, it must be returned to the factory for evaluation. OMEGA’s Customer Service Department will issue an Authorized Return (AR) number immediately upon phone or written request. Upon examination by OMEGA, if the unit is found to be defective, it will be repaired or replaced at no charge. OMEGA’s WARRANT Y does not apply to defects resulting from any action of the purchaser, including but not limited to mishandling, improper interfacing, operation outside of design limits, improper repair, or unauthorized modification. T his WARRANT Y is VOID if the unit shows evidence of having been tampered with or shows evidence of having been damaged as a result of excessive corrosion; or current, heat, moisture or vibration; improper specification; misapplication; misuse or other operating conditions outside of OMEGA’s control. Components in which wear is not warranted, include but are not limited to contact points, fuses, and triacs.OMEGA is pleased to offer suggestions on the use of its various products. However, OMEGA neither assumes responsibility for any omissions or errors nor assumes liability for any damages that result from the use of its products in accordance with information provided by OMEGA, either verbal or written. OMEGA warrants only that the parts manufactured by the company will be as specified and free of defects. OMEGA MAKES NO OTHER WARRANTIES OR REPRESENTATIONS OF ANY KIND WHATSOEVER, EXPRESSED OR IMPLIED, EXCEPT THAT OF TITLE, AND ALL IMPLIED WARRANTIES INCLUDING ANY WARRANTY OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE ARE HEREBY DISCLAIMED. LIMITATION OF LIABILITY: The remedies of purchaser set forth herein are exclusive, and the total liability of OMEGA with respect to this order, whether based on contract, warranty, negligence, indemnification, strict liability or otherwise, shall not exceed the purchase price of the component upon which liability is based. In no event shall OMEGA be liable for consequential, incidental or special damages.CONDITIONS: Equipment sold by OMEGA is not intended to be used, nor shall it be used: (1) as a “Basic Component” under 10 CFR 21 (NRC), used in or with any nuclear installation or activity; or (2) in medical applications or used on humans. Should any Product(s) be used in or with any nuclear installation or activity, medical application, used on humans, or misused in any way, OMEGA assumes no responsibility as set forth in our basic WARRANTY / DISCLAIMER language, and, additionally, purchaser will indemnify OMEGA and hold OMEGA harmless from any liability or damage whatsoever arising out of the use of the Product(s) in such a manner.RETURN REQUESTS/INQUIRIESDirect all warranty and repair requests/inquiries to the OMEGA Customer Service Department. BEFORE RET URNING ANY PRODUCT (S) T O OMEGA, PURCHASER MUST OBT AIN AN AUTHORIZED RETURN (AR) NUMBER FROM OMEGA’S CUSTOMER SERVICE DEPARTMENT (IN ORDER TO AVOID PROCESSING DELAYS). The assigned AR number should then be marked on the outside of the return package and on any correspondence.T he purchaser is responsible for shipping charges, freight, insurance and proper packaging to prevent breakage in transit.OMEGA’s policy is to make running changes, not model changes, whenever an improvement is possible. This affords our customers the latest in technology and engineering.OMEGA is a registered trademark of OMEGA ENGINEERING, INC.© Copyright 2015 OMEGA ENGINEERING, INC. All rights reserved. This document may not be copied, photocopied, reproduced, translated, or reduced to any electronic medium or machine-readable form, in whole or in part, without the prior written consent of OMEGA ENGINEERING, INC.FOR WARRANTY RETURNS, please have the following information available BEFORE contacting OMEGA:1. Purchase Order number under which the product was PURCHASED,2.M odel and serial number of the product under warranty, and 3. R epair instructions and/or specific problems relative to the product.FOR NON-WARRANTY REPAIRS, consult OMEGA for current repair charges. Have the following information available BEFORE contacting OMEGA:1. P urchase Order number to cover the COST of the repair,2. Model and serial number of theproduct, and3. Repair instructions and/or specific problemsrelative to the product.Where Do I Find Everything I NeedforProcess Measurement and Control?OMEGA…Of Course!Shop online at SMTEMPERATUREThermocouple, RTD & Thermistor Probes, Connectors, Panels & AssembliesWire: Thermocouple, RTD & ThermistorCalibrators & Ice Point References Recorders, Controllers & Process MonitorsInfrared Pyrometers PRESSURE, STRAIN AND FORCETransducers & Strain GagesLoad Cells & Pressure Gages Displacement Transducers Instrumentation & Accessories FLOW/LEVEL Rotameters, Gas Mass Flowmeters & Flow ComputersAir V elocity IndicatorsTurbine/Paddlewheel Systems Totalizers & Batch ControllerspH/CONDUCTIVITYpH Electrodes, Testers & Accessories Benchtop/Laboratory Meters Controllers, Calibrators, Simulators & PumpsIndustrial pH & Conductivity Equipment DATA ACQUISITIONData Acquisition & Engineering Software Communications-Based Acquisition SystemsPlug-in Cards for Apple, IBM & CompatiblesData Logging Systems Recorders, Printers & Plotters HEATERSHeating CableCartridge & Strip Heaters Immersion & Band Heaters Flexible HeatersLaboratory Heaters ENVIRONMENTAL MONITORING AND CONTROL Metering & Control Instrumentation RefractometersPumps & TubingAir, Soil & Water Monitors Industrial Water & Wastewater TreatmentpH, Conductivity & Dissolved Oxygen InstrumentsM-5582/0516。

WCH SeriesWashdown / Corrosion ResistantIndustrial / Commercial Electric Unit HeaterOwner’s ManualThis manual covers installation, operation and maintenance. Read carefully before attempting to install, operate or service the WCH Series Unit Heater.IMPORTANT INSTRUCTIONSSAVE THESE INSTRUCTIONS FOR FUTURE REFERENCEHAZLOC HEATERS INC.#1, 666 Goddard Ave. NE • Calgary, AB • T2K 5X3 • CanadaTel: +1‐403‐730‐2488 • Toll Free (Canada & U.S.): 1‐866‐701‐HEAT (4328)Fax: +1‐403‐730‐2482IMPORTANT INSTRUCTIONSInstallation and maintenance personnel should familiarize themselves with this manual and all the IMPORTANT INSTRUCTIONS before installing or working on this heater to avoid potential unsafe conditions, severe property damage, personal injury or death.1. Read all instructions before installing andoperating this heater.2. Verify that the supply voltage and phase tothe heater matches the nameplate ratingbefore energizing.3. Potentially lethal voltages are present. Besure to lock the branch circuit disconnectswitch in the OFF position and tag thecircuit “Out for Maintenance” beforeworking on this equipment.4. Keep electrical enclosure cover tightlyclosed while in operation.5. Hazard of Electric Shock. Heater must begrounded in accordance with both local andnational codes.6. This heater should be installed by a licensedelectrician familiar with all applicablenational and local codes having jurisdiction.It is the responsibility of the installer toverify the safety and suitability of theinstallation.7. Disassembly of the unit for installation isnot required or authorized.8. Replacement electrical components mustbe obtained from the factory in order tomaintain any applicable Agency Listings. 9. Use this heater only as described in thismanual. Any other use is not recommended by the manufacturer and may result in fire, electric shock or personal injury.10. The heater and discharge air are hot whenin use. To avoid burns, do not let bare skintouch hot surfaces.11. To prevent a possible fire, do not block orallow foreign objects to enter air intakes orexhaust in any manner. 12. Risk of fire due to high temperatures. Keepelectrical cords, drapery, furnishings,insulation and other combustibles at least 3feet (0.9m) from the front of the heater and away from the sides, rear and top.13. Installation minimum mounting clearancesspecified both on heater nameplate and inthis owner’s manual must be maintained.14. Use copper wire rated 90°C min. for supplyconnections.15. This heater should not be used inpotentially explosive atmospheres. Do notuse in areas where gasoline, paint, orflammable liquids are used or stored. 16. Do not use outdoors. Heater may bewashed down with water for cleaning. Donot use high pressure cleaning systems. 17. Risk of fire. Do not use as a residential orhousehold heater.18. Heater is designed to be controlled by aroom thermostat located within theoperating space that will control the roomtemperature below 80°F (26.7°C). Do notallow heater to cycle on the built‐on limitcontrols.19. The heaters are designed for permanentwall or ceiling mounting in a horizontalposition only.20. Do not operate heater after it malfunctions.Disconnect power at service panel and have heater inspected by a reputable electricianbefore reusing.21. SAVE THESE INSTRUCTIONS.WARRANTY WILL BE VOID IF INSTRUCTIONS ARE NOT FOLLOWED.INSTALLATION INSTRUCTIONSRISK OF FIRE / EXPLOSION∙This heater should not be used in potentially explosive atmospheres. Do not use in areas where gasoline, paint or flammable liquids are used or stored.∙Keep electrical cords, drapery, furnishings, insulation and other combustibles at least 3 feet (0.9m) from the front of the heater and away from the sides, rear and top.∙Installation minimum clearances specified both on the heater nameplate and in the owner’s manual must be maintained.∙Do not use as a residential or household heater.These air heaters are designed for comfort heating and should not be used in ambient temperatures exceeding 80ºF (26.7ºC). They are to be permanently mounted to the wall or ceiling for horizontal discharge. The unit is designed to give years of safe, trouble‐free operation when properly installed and maintained.A. Site Selection:The heaters are intended for elevated mounting locations so that they blow warm air down to the floor area. A mounting height should be selected so that the heater is out of the way of possible moving equipment or personnel, yet low enough to deliver warm air to the selected area. See the mechanical installation section for recommended installation heights.Heater airflow should be directed to areas of greatest heat loss. In general, greater numbers of small heaters will provide more uniform and even heat distributions than a few large ones. In order to help move heated air around the room, multiple heaters should be spaced out and direct air in a circular pattern around the room perimeter such that each heater supports the next heater’s airstreams. See Figures 1 & 2 for some typical airflow pattern arrangements:Large room with exposed walls and roof :B. Mechanical Installation:These heaters are to be mounted for horizontal discharge only. Mounting is to be accomplished by using either (4) 3/8x16 threaded rods (supplied by others) or the stainless steel swivel mounting bracket factory supplied with the heater. Lock washers should be used on all mounting nuts and bolts to ensure they don’t vibrate or work loose due to fan vibration or other vibration transmitted to the heater.The supporting structure that the heater is attached to must have adequate strength to safely support the heater. The heater dimensions and maximum unit weights are: TABLE 1:KW CabinetSizeX Y Z Weight<= 12 115.50”(394mm)21.50”(546mm)13.00”(330mm)56 lbs(25 kg)12.1 to 47 226.00”(660mm)28.00”(711mm)20.00”(508mm)115 lbs(52 kg)Select a location for installation such that the outlet air is not directed at an adjacent wall. Louvered grills can be installed to deflect the discharge air up, down or sideways. TABLE 2:KW CabinetSizeAir VolumeAirThrowOutletVelocity<= 7.5 1700 CFM(1189 cubic m/hr)26 Ft(8 m)760 FPM(232 m/min)7.6 to 12 11450 CFM(2463 cubic m/hr)43 Ft(13 m)1575 FPM(481 m/min)12.1 to 47 22400 CFM(4077 cubic m/hr)50 Ft(15 m)1200 FPM(366 m/min)Once an acceptable location has been determined, see thefollowing instructions to complete the mechanicalinstallation:1. To ensure proper heating of floor surfaces,observethefollowingrecommendedmountingheightlimitations (tobottom of heater):TABLE 3: Max. Mounting Height from Floor, feet (m)<= 5 KW 5.1 to 10KW10.1 to 20KW20.1 to 47KW10’ (3m) 15’ (4.6m) 20’ (6.1m) 25‘ (7.6m)2. Install the heater at least 6 feet (1.8m) from the floorand 8” (203mm) from the wall or ceiling.3. Mount heater using one of the following two options:Standardceiling or wall mount using the swivel bracket:(See Table 4 on next page for tabulated dimensions)Ceiling or wall mount using swivel bracket (cont):TABLE 4:KW BHBLM <= 124.25” (108mm) 19.50” (495mm) 8.50” (216mm) 12.1 to 477.125” (181mm) 22.25”(565mm)3.25” (83mm)Optional Ceiling Installation using 4 threaded rods:For ceiling installation using threaded rods, secure the four rods to the ceiling using locknuts.INSTALLATION INSTRUCTIONS ‐ ContinuedELECTRIC SHOCK HAZARDElectrical installation should be made by a qualified licensed electrician. Wiring procedures, connectionsand grounding shall be in accordance with the national and local codes having jurisdiction.C. Electrical Installation:Follow these instructions to complete the electrical installation:1. External branch circuit protection is required. See nameplate ratings and follow Code recommendations.2. Follow the national and local electrical and building codes related to the installation and intended use of the heater.3. When doing any work on a heater, including the initial electrical connection, disconnect the electrical supply at the mainbranch circuit switch, and lock the switch in the off (open) position. Tag the circuit "Out for Maintenance" to prevent potential lethal shock hazards.4. Confirm that the electrical power supply matches the nameplate voltage, phase, amperage and frequency rating of theheater to be connected.5. Field supply conductors must be sized for at least 125% of the circuit current. The circuit current in amps iscalculated as follows: Single Phase Amperage = Circuit kW x 1000 Circuit VoltageThree Phase Amperage = Circuit kW x 1000 1.732 x Circuit Voltage6. Use copper conductors rated 90°C minimum with watertight conduit fittings.7. Field wiring to the heater must comply with all branch circuit requirements of any national and local codes havingjurisdiction.8. Proper installation of the heater requires that an adequate grounding conductor be connected to the ground terminal.This terminal marked with the letter “G” and is located on the inside of the control enclosure.9. A wiring diagram is supplied with each heater and is located on the inside surface of the control enclosure cover. 10. Check and confirm all connections are securely tightened. Remove any foreign objects from the control boxandcloseaccess door.11. See section titled “Operating Instructions” before energizing the heater.OPERATING INSTRUCTIONSRISKOFFIRE / EXPLOSION∙ This heater should not be used in potentially explosive atmospheres. Do not use in areas where gasoline,paint or flammable liquids are used or stored.∙ Keep electrical cords, drapery, furnishings, insulation and other combustibles at least 3 feet (0.9m) from the front of the heater and away from the sides, rear and top.∙ To prevent a possible fire, do not block or allow foreign objects to enter air intakes or exhaust inany manner.ELECTRIC SHOCK HAZARD∙ Keep electrical enclosure cover tightly closed while in operation.∙ Do not operate heater after a malfunction. Disconnect power at service panel and have heaterinspected by a reputable electrician before reusing.∙ Use this heater only as described in this manual. Any other use is not recommended by the manufacturer and may result in fire, electric shock or personal injury.RISK OF INJURY / BURN∙ The heater and discharge air are hot when in use. To avoid burns, do not let bare skin touch hotsurfaces.∙ Do not attempt to service or clean heater while unit is operating as there is a hazard from electricshock, injury from operating fan blade and burns from hot heating elements.The unit is designed to give years of safe, trouble ‐free operation when properly installed and maintained. Please read the following guidelines to ensure reliable operation:1. Confirm proper mechanical and electrical installation before operation of the heater.2. This unit heater may be operated at ambient temperatures of 80°F (26.7°C) or less.3. Make sure the wiring enclosure cover is closed securely.4. Set the temperature control thermostat to a setting above the current room temperature.5. If the heater is provided with a selector switch, it must be in the ON position.6. Energize the heater electrical supply circuit.7. The heater and fan should come on.8.Check for the correct fan blade rotation. If air is not discharging through the louvers, interchange any two of the three field supplied power leads on three phase units only.9. Since the heater is equipped with a built ‐in time delay relay, the fan will remain in operation for about a minute to coolthe elements after the heater is switched off either by the thermostat or the selector switch. Do not turn the heater off at the main disconnect switch, as this will prevent the fan from cooling elements.OPERATING INSTRUCTIONS ‐ ContinuedRISKOFFIRE / EXPLOSION∙This heater should not be used in potentially explosive atmospheres. Do not use in areas where gasoline, paint or flammable liquids are used or stored.∙Keep electrical cords, drapery, furnishings, insulation and other combustibles at least 3 feet(0.9m) from the front of the heater and away from the sides, rear and top.∙To prevent a possible fire, do not block or allow foreign objects to enter air intakes or exhaust in any manner.ELECTRIC SHOCK HAZARD∙Keep electrical enclosure cover tightly closed while in operation.∙Do not operate heater after a malfunction. Disconnect power at service panel and have heater inspected by a reputable electrician before reusing.∙Use this heater only as described in this manual. Any other use is not recommended by the manufacturer and may result in fire, electric shock or personal injury.RISK OF INJURY / BURN∙The heater and discharge air are hot when in use. To avoid burns, do not let bare skin touch hot surfaces.∙Do not attempt to service or clean heater while unit is operating as there is a hazard from electric shock, injury from operating fan blade and burns from hot heating elements.The unit is designed to give years of safe, trouble‐free operation when properly installed and maintained. Please read the following guidelines to ensure reliable operation:10.During normal operation, the limit controls are not supposed to cycle the heater ON and OFF. If frequent cycling ofthe limit controls occurs, verify the minimum mounting clearances are maintained, proper operation of the roomthermostat and that there is no visible obstruction to the heater inlet or outlet. If this does not eliminate the limitcontrol cycling, the heater must be examined by qualified service personnel before further operation to determine the cause.11.Heater may be provided with a manual reset limit control (order code M). This limit control disables the electricalfunction of the heater whenever excessive temperatures are present. If the manual reset limit control is suspected of causing heater non‐function then the heater must be disconnected from the heater electrical supply and examined bya qualified service personnel. The personnel must determine the cause of the excessive temperatures, verify theminimum mounting clearances, verify proper orientation of the heater, verify proper orientation of the roomthermostat, or if there are any obstructions or damage to the heater or heater inlet or heater outlet. The manualreset limit control may be reset, after determining the cause, by removing the electrical enclosure cover and pressing on the manual reset button. The location of the manual reset button within the electrical enclosure is shown in the Maintenance Instructions.12.Check out and report any unusual or questionable operating characteristics, such as noise, vibration, etc.13.Set the temperature control thermostat to the desired room temperature setting and place unit in service.MAINTENANCE INSTRUCTIONSELECTRIC SHOCK HAZARD∙ Potentially lethal voltages are present. Be sure to lock the branch circuit disconnect switch in theOFF position and tag the circuit “Out for Maintenance” before working on this equipment.RISK OF INJURY / BURN∙ Do not attempt to service or clean heater while unit is operating as there is a hazard from electricshock, injury from operating fan blade and burns from hot heating elements. ∙ Maintenance and repair must be performed by qualified service personnel only.Electrical:1. Inspect all terminal connections, contactors and conductor insulation for damage, looseness, fraying, etc., as applicable. Tighten any loose terminals and replace or repair wire that has damaged ordeteriorated insulation. Replace any contactor that has contacts that are badly pitted, welded together, or burned.2. If reduced heat output is suspected, perform themechanical checks. If low heat output is still suspected after completing the mechanical checks, verify the condition of the heating elements by visual inspection and by using an amperage meter to check the current draw of each input line. Adjust the room thermostat to its highest temperature to energize heater. All input lines should draw approximately equal current which should agree with the nameplate rating. If they do not, one or more of the heating elements could be burned out and should be replaced. 3. For heaters supplied with the optional manual reset limit control (order code ‘M’), the electrical enclosure cover must be opened to gain access to the reset button. See below to locate the reset button:Mechanical:1. Before cleaning or servicing, ensure power has been turned off at the service panel and the heating elements are cool.2. Annually check the tightness of all visible bolts and nuts, in particular the support structure bolts and nuts.3. Periodically, check the motor, fan, discharge openings, intake openings, heating elements and control compartment for cleanliness. If necessary, clean by using a vacuum or compressed air. Be careful not to bend or distort the fan blade propeller.4. Check fan motor for smooth running operation. Any unusual noise or vibration must be investigated and rectified.5. The electric motors are permanently lubricated and thermally protected. Check for smooth and quiet operation at all inspections. Replace motor if excessive bearing play is detected.6. The heater may be washed downwithwater. Do not use high pressure cleaning systems.。