AREMA_MRE_2010_TOC-Vol2_Ch8

Förster resonance energy transferFrom Wikipedia, the free encyclopediaJump to: navigation, searchJablonski diagram of FRET with typical timescales indicatedFörster resonance energy transfer (FRET), fluorescence resonance energy transfer (FRET), resonance energy transfer (RET) orelectronic energy transfer (EET) is a mechanism describing energy transfer between two light-sensitive molecules (chromophores).[1] A donor chromophore, initially in its electronic excited state, may transfer energy to an acceptor chromophore through nonradiative dipole–dipole coupling.[2] The efficiency of this energy transfer is inversely proportional to the sixth power of the distance between donor and acceptor, making FRET extremely sensitive to small changes in distance.[3]Measurements of FRET efficiency can be used to determine if two fluorophores are within a certain distance of each other.[4] Such measurements are used as a research tool in fields including biology and chemistry.FRET is analogous to near-field communication, in that the radius of interaction is much smaller than the wavelength of light emitted. In the near-field region, the excited chromophore emits a virtual photon that is instantly absorbed by a receiving chromophore. These virtual photons are undetectable, since their existence violates the conservation of energy and momentum, and hence FRET is known as a radiationless mechanism. Quantum electrodynamical calculations have been used to determine that radiationless (FRET) and radiative energy transfer are the short- and long-range asymptotes of a single unified mechanism.[5][6]Contents[hide]∙ 1 Terminology∙ 2 Theoretical basis∙ 3 Experimental co nfirmation of the Förster resonance energy transfer theory∙ 4 Methods to measure FRET efficiencyo 4.1 Sensitized emissiono 4.2 Photobleaching FRETo 4.3 Lifetime measurements∙ 5 Fluorophores used for FRETo 5.1 CFP-YFP pairso 5.2 BRETo 5.3 Homo-FRET∙ 6 Applicationso 6.1 Biology∙7 Other methods∙8 See also∙9 References∙10 External linksTerminology[edit]Förster resonance energy transfer is named after the German scientist Theodor Förster.[7] When both chromophores are fluorescent, the term "fluorescence resonance energy transfer" is often used instead, although the energy is not actually transferred by fluorescence.[8][9]In order to avoid an erroneous interpretation of the phenomenon that is always a nonradiative transfer of energy (even when occurring between two fluorescent chromophores), the name "Förster resonance energy transfer" is preferred to "fluorescence resonance energy transfer;" however, the latter enjoys common usage in scientific literature.[10] It should also be noted that FRET is not restricted to fluorescence. It can occur in connection with phosphorescence as well.[8]Theoretical basis[edit]The FRET efficiency () is the quantum yield of the energy transfer transition, i.e. the fraction of energy transfer event occurring per donor excitation event:[11]where is the rate of energy transfer, the radiative decayrate, and the 's are the rate constants of any other de-excitation pathways.[12]The FRET efficiency depends on many physical parameters that can be grouped as follows:∙The distance between the donor and the acceptor (typically in the range of 1-10 nm)∙The spectral overlap of the donor emission spectrum and the acceptor absorption spectrum.∙The relative orientation of the donor emission dipole moment and the acceptor absorption dipole moment.depends on the donor-to-acceptor separation distance with an inverse 6th power law due to the dipole-dipole coupling mechanism:with being the Förster distance of this pair of donor andacceptor, i.e. the distance at which the energy transfer efficiency is 50%.[12]The Förster distance depends on the overlap integral of the donor emission spectrum with the acceptor absorption spectrum and their mutual molecular orientation as expressed by the following equation.[13][14]where is the fluorescence quantum yield of the donor in the absence of the acceptor, κ2 is the dipole orientation factor, is the refractive index of the medium, is Avogadro's number, and is the spectral overlap integral calculated aswhere is the normalized donor emission spectrum, and is the acceptor molar extinction coefficient.[15] The orientation factor κ is given by,Where denotes the normalized transition dipole moment of therespective fluorophore and denotes the normalized inter-fluorophore displacement. κ2 =2/3 is often assumed. This value is obtained when both dyes are freely rotating and can be considered to beisotropically oriented during the excited state lifetime. If either dye is fixed or not free to rotate, then κ2 =2/3 will not be a valid assumption. In most cases, however, even modest reorientation of the dyes results in enough orientational averaging that κ2 = 2/3 doesnot result in a large error in the estimated energy transfer distance due to the sixth power dependence of R0 on κ2. Even when κ2 is quite different from 2/3 the error can be associated with a shift in R0 and thus determinations of changes in relative distance for a particular system are still valid. Fluorescent proteins do not reorient on a timescale that is faster than their fluorescence lifetime. In this case 0 ≤ κ2≤ 4.[15]The FRET efficiency relates to the quantum yield and the fluorescence lifetime of the donor molecule as follows:[16]where and are the donor fluorescence lifetimes in the presenceand absence of an acceptor, respectively, or aswhere and are the donor fluorescence intensities with and without an acceptor, respectively.Experimental confirmation of the Förster resonance energy transfer theory[edit]The inverse sixth-power distance dependence of Förster resonance energy transfer was experimentally confirmed by Wilchek, Edelhoch and Brand[17][18] using tryptophyl peptides. Stryer, Haugland and Yguerabide[19] also experimentally demonstrated the theoretical dependence ofFörster resonance ene rgy transfer on the overlap integral by using a fused indolosteroid as a donor and a ketone as an acceptor. However, a lot of contradictions of special experiments with the theory was oserved. The reason is that the theory has approximate character and gives overstimated distances of 50-100 Angstrems (Vekshin N.L. Energy Transfer in Macromolecules, SPIE, 1997; Vekshin N.L. Photonics of Biopolymers, Springer, 2002).Methods to measure FRET efficiency[edit]In fluorescence microscopy, fluorescence confocal laser scanning microscopy, as well as in molecular biology, FRET is a useful tool to quantify molecular dynamics in biophysics and biochemistry, such as protein-protein interactions, protein–DNA interactions, and protein conformational changes. For monitoring the complex formation between two molecules, one of them is labeled with a donor and the other with an acceptor. The FRET efficiency is measured and used to identify interactions between the labeled complexes. There are several ways of measuring the FRET efficiency by monitoring changes in the fluorescence emitted by the donor or the acceptor.[20]Sensitized emission[edit]One method of measuring FRET efficiency is to measure the variationin acceptor emission intensity.[14] When the donor and acceptor are in proximity (1–10 nm) due to the interaction of the two molecules, the acceptor emission will increase because of the intermolecularFRET from the donor to the acceptor. For monitoring protein conformational changes, the target protein is labeled with a donor and an acceptor at two loci. When a twist or bend of the protein brings the change in the distance or relative orientation of the donor and acceptor, FRET change is observed. If a molecular interaction or a protein conformational change is dependent on ligand binding, this FRET technique is applicable to fluorescent indicators for the ligand detection.Photobleaching FRET[edit]FRET efficiencies can also be inferred from the photobleaching rates of the donor in the presence and absence of an acceptor.[14] This method can be performed on most fluorescence microscopes; one simply shines the excitation light (of a frequency that will excite the donor but not the acceptor significantly) on specimens with and without the acceptor fluorophore and monitors the donor fluorescence (typically separated from acceptor fluorescence using a bandpass filter) over time. The timescale is that of photobleaching, which is seconds to minutes, with fluorescence in each curve being given bywhere is the photobleaching decay time constant and depends on whether the acceptor is present or not. Since photobleaching consists in the permanent inactivation of excited fluorophores, resonance energy transfer from an excited donor to an acceptor fluorophore prevents the photobleaching of that donor fluorophore, and thus high FRET efficiency leads to a longer photobleaching decay time constant:where and are the photobleaching decay time constants of thedonor in the presence and in the absence of the acceptor, respectively. (Notice that the fraction is the reciprocal of that used for lifetime measurements).This technique was introduced by Jovin in 1989.[21] Its use of anentire curve of points to extract the time constants can give it accuracy advantages over the other methods. Also, the fact that time measurements are over seconds rather than nanoseconds makes it easierthan fluorescence lifetime measurements, and because photobleaching decay rates do not generally depend on donor concentration (unless acceptor saturation is an issue), the careful control of concentrations needed for intensity measurements is not needed. It is, however, important to keep the illumination the same for the with- and without-acceptor measurements, as photobleaching increases markedly with more intense incident light.Lifetime measurements[edit]FRET efficiency can also be determined from the change in the fluorescence lifetime of the donor.[14] The lifetime of the donor will decrease in the presence of the acceptor. Lifetime measurements of FRET are used in Fluorescence-lifetime imaging microscopy.Fluorophores used for FRET[edit]If the linker is intact, excitation at the absorbance wavelength of CFP (414nm) causes emission by YFP (525nm) due to FRET. If the linker is cleaved by a protease, FRET is abolished and emission is at the CFP wavelength (475nm).CFP-YFP pairs[edit]One common pair fluorophores for biological use is a cyan fluorescent protein (CFP) – yellow fluorescent protein (YFP) pair.[22] Both are color variants of green fluorescent protein (GFP). Labeling with organic fluorescent dyes requires purification, chemical modification, and intracellular injection of a host protein. GFP variants can be attached to a host protein by genetic engineering which can be more convenient. Additionally, a fusion of CFP and YFP linked by a protease cleavage sequence can be used as a cleavage assay.[23]BRET[edit]A limitation of FRET is the requirement for external illumination to initiate the fluorescence transfer, which can lead to background noise in the results from direct excitation of the acceptor or to photobleaching. To avoid this drawback, Bioluminescence Resonance Energy Transfer (or BRET) has been developed.[24] This technique uses a bioluminescent luciferase (typically the luciferase from Renilla reniformis) rather than CFP to produce an initial photon emission compatible with YFP.Homo-FRET[edit]In general, "FRET" refers to situations where the donor and acceptor proteins (or "fluorophores") are of two different types. In many biological situations, however, researchers might need to examine the interactions between two, or more, proteins of the same type—or indeed the same protein with itself, for example if the protein folds or forms part of a polymer chain of proteins[25] or for other questions of quantification in biological cells.[26]Obviously, spectral differences will not be the tool used to detect and measure FRET, as both the acceptor and donor protein emit light with the same wavelengths. Yet researchers can detect differences in the polarisation between the light which excites the fluorophores andthe light which is emitted, in a technique called FRET anisotropy imaging; the level of quantified anisotropy (difference in polarisation between the excitation and emission beams) then becomes an indicative guide to how many FRET events have happened.[27]Applications[edit]Biology[edit]FRET has been used to measure distance and detect molecular interactions in a number of systems and has applications in biology and chemistry.[28] FRET can be used to measure distances between domains in a single protein and therefore to provide information about protein conformation.[29] FRET can also detect interaction between proteins.[30] Applied in vivo, FRET has been used to detect the location and interactions of genes and cellular structures including intergrins and membrane proteins.[31] FRET can be used to obtain information about metabolic or signaling pathways.[32] FRET is also used to study lipid rafts in cell membranes.[33]FRET and BRET are also the common tools in the study of biochemical reaction kinetics and molecular motors.The applications of Fluorescence Resonance Energy Transfer (FRET) have expanded tremendously in the last 25 years, and the technique has become a staple technique in many biological and biophysical fields. FRET can be used as spectroscopic ruler in various areas such as structural elucidation of biological molecules and their interactions in vitro assays, in vivo monitoring in cellular research, nucleic acid analysis, signal transduction, light harvesting and metallic nanomaterial etc. Based on the mechanism of FRET a variety of novel chemical sensors and biosensors have been developed.[34]Other methods[edit]A different, but related, mechanism is Dexter Electron Transfer.An alternative method to detecting protein–protein proximity is the bimolecular fluorescence complementation (BiFC) where two halves of a YFP are fused to a protein. When these two halves meet they form a fluorophore after about 60 s – 1 hr.[35]See also[edit]∙Förster coupling∙Surface energy transfer∙Dexter electron transfer∙Time-resolved fluorescence energy transferReferences[edit]1.Jump up ^ Cheng, Ping-Chin (2006). "The Contrast Formation in OpticalMicroscopy". In Pawley, James B. Handbook Of Biological Confocal Microscopy(3rd ed.). New York, NY: Springer. pp. 162–206. doi:10.1007/978-0-387-45524-2_8. ISBN 978-0-387-25921-5.2.Jump up ^ Helms, Volkhard (2008). "Fluorescence Resonance Energy Transfer".Principles of Computational Cell Biology. Weinheim: Wiley-VCH. p. 202.ISBN 978-3-527-31555-0.3.Jump up ^ Harris, Daniel C. (2010). "Applications of Spectrophotometry".Quantitative Chemical Analysis (8th ed.). New York: W. H. Freeman and Co.pp. 419–44. ISBN 978-1-4292-1815-3.4.Jump up ^ Zheng, Jie (2006). "Spectroscopy-Based Quantitative FluorescenceResonance Energy Transfer Analysis". In Stockand, James D.; Shapiro, MarkS. Ion Channels: Methods and Protocols. Methods in Molecular Biology,Volume 337. Totowa, NJ: Humana Press. pp. 65–77. doi:10.1385/1-59745-095-2:65. ISBN 978-1-59745-095-9.5.Jump up ^ Andrews, David L. (1989). "A unified theory of radiative andradiationless molecular energy transfer". Chemical Physics135 (2): 195–201. Bibcode:1989CP....135..195A. doi:10.1016/0301-0104(89)87019-3.6.Jump up ^ Andrews, David L; Bradshaw, David S (2004). "Virtual photons,dipole fields and energy transfer: A quantum electrodynamical approach".European Journal of Physics25 (6): 845. doi:10.1088/0143-0807/25/6/017.7.Jump up ^Förster, Theodor (1948). "Zwischenmolekulare Energiewanderung undFluoreszenz" [Intermolecular energy migration and fluorescence]. Annalender Physik (in German) 437: 55–75. Bibcode:1948AnP...437...55F.doi:10.1002/andp.19484370105.8.^ Jump up to: a b Valeur, Bernard; Berberan-Santos, Mario (2012). "ExcitationEnergy Transfer". Molecular Fluorescence: Principles and Applications, 2nded. Weinheim: Wiley-VCH. pp. 213–261. doi:10.1002/9783527650002.ch8.ISBN 9783527328376.9.Jump up ^ FRET microscopy tutorial from Olympus10.Jump up ^Glossary of Terms Used in Photochemistry (3rd ed.). IUPAC. 2007.p. 340.11.Jump up ^ Moens, Pierre. "Fluorescence Resonance Energy Transferspectroscopy". Retrieved July 14, 2012.12.^ Jump up to: a b Schaufele, Fred; Demarco, Ignacio; Day, Richard N. (2005)."FRET Imaging in the Wide-Field Microscope". In Periasamy, Ammasi; Day, Richard. Molecular Imaging: FRET Microscopy and Spectroscopy. Oxford:Oxford University Press. pp. 72–94. doi:10.1016/B978-019517720-6.50013-4.ISBN 978-0-19-517720-6.13.Jump up ^Förster, Th. (1965). "Delocalized Excitation and ExcitationTransfer". In Sinanoglu, Oktay. Modern Quantum Chemistry. IstanbulLectures. Part III: Action of Light and Organic Crystals3. New York and London: Academic Press. pp. 93–137. Retrieved 2011-06-22.14.^ Jump up to: a b c d Clegg, Robert (2009). "Förster resonance energytransfer—FRET: what is it, why do it, and how it's done". In Gadella,Theodorus W. J. FRET and FLIM Techniques. Laboratory Techniques inBiochemistry and Molecular Biology, Volume 33. Elsevier. pp. 1–57.doi:10.1016/S0075-7535(08)00001-6. ISBN 978-0-08-054958-3.15.^ Jump up to: a b Demchenko, Alexander P. (2008). "Fluorescence DetectionTechniques". Introduction to Fluorescence Sensing. Dordrecht: Springer.pp. 65–118. doi:10.1007/978-1-4020-9003-5_3. ISBN 978-1-4020-9002-8. 16.Jump up ^ Majoul, Irina; Jia, Yiwei; Duden, Rainer (2006). "PracticalFluorescence Resonance Energy Transfer or Molecular Nanobioscopy of Living Cells". In Pawley, James B. Handbook Of Biological Confocal Microscopy (3rd ed.). New York, NY: Springer. pp. 788–808. doi:10.1007/978-0-387-45524-2_45. ISBN 978-0-387-25921-5.17.Jump up ^ Template:ISRAEL JOURNAL OF CHEMISTRY. Vol. 1. No. Sa. 196318.Jump up ^ {Edelhoch, H., Brand, L., Wilchek, M. (1967). "Fluorescencestudies with tryptophyl peptides". Biochemistry 6 (2): 547–559.doi:10.1021/bi00854a024. PMID 6047638}19.Jump up ^ Lakowicz, Joseph R., ed. (1991). Principles. New York: PlenumPress. p. 172. ISBN 978-0-306-43875-2.20.Jump up ^ "Fluorescence Resonance Energy Transfer Protocol". WellcomeTrust. Retrieved 24 June 2012.[dead link]21.Jump up ^Szöllősi, János; Alexander, Denis R. (2007). "The Application ofFluorescence Resonance Energy Transfer to the Investigation ofPhosphatases". In Klumpp, Susanne; Krieglstein, Josef. ProteinPhosphatases. Methods in Enzymology, Volume 366. Amsterdam: Elsevier.pp. 203–24. doi:10.1016/S0076-6879(03)66017-9. ISBN 978-0-12-182269-9. 22.Jump up ^ Periasamy, Ammasi (July 2001). "Fluorescence resonance energytransfer microscopy: a mini review". Journal of Biomedical Optics6 (3): 287–291. Bibcode:2001JBO.....6..287P. doi:10.1117/1.1383063.PMID 11516318.23.Jump up ^ Nguyen, AW; Daugherty, PS (March 2005). "Evolutionaryoptimization of fluorescent proteins for intracellular FRET.". Naturebiotechnology23 (3): 355–60. doi:10.1038/nbt1066. PMID 15696158.24.Jump up ^ Bevan, Nicola; Rees, Stephen (2006). "Pharmaceutical Applicationsof GFP and RCFP". In Chalfie, Martin; Kain, Steven R. Green FluorescentProtein: Properties, Applications and Protocols. Methods of Biochemical Analysis, Volume 47 (2nd ed.). Hoboken, NJ: John Wiley & Sons. pp. 361–90. doi:10.1002/0471739499.ch16. ISBN 978-0-471-73682-0.25.Jump up ^ Gautier, I.; Tramier, M.; Durieux, C.; Coppey, J.; Pansu, R.B.;Nicolas, J.-C.; Kemnitz, K.; Coppey-Moisan, M. (2001). "Homo-FRETMicroscopy in Living Cells to Measure Monomer-Dimer Transition of GFP-Tagged Proteins". Biophysical Journal80 (6): 3000–8.Bibcode:2001BpJ....80.3000G. doi:10.1016/S0006-3495(01)76265-0.PMC 1301483. PMID 11371472.26.Jump up ^ Bader, Arjen N.; Hofman, Erik G.; Voortman, Jarno; Van Bergen EnHenegouwen, Paul M.P.; Gerritsen, Hans C. (2009). "Homo-FRET ImagingEnables Quantification of Protein Cluster Sizes with SubcellularResolution". Biophysical Journal97 (9): 2613–22.Bibcode:2009BpJ....97.2613B. doi:10.1016/j.bpj.2009.07.059. PMC 2770629.PMID 19883605.27.Jump up ^ Gradinaru, Claudiu C.; Marushchak, Denys O.; Samim, Masood;Krull, Ulrich J. (2010). "Fluorescence anisotropy: From single molecules to live cells". The Analyst135 (3): 452–9. Bibcode:2010Ana...135..452G.doi:10.1039/b920242k. PMID 20174695.28.Jump up ^ Lakowicz, Joseph R. (1999). Principles of fluorescencespectroscopy (2nd ed.). New York, NY: Kluwer Acad./Plenum Publ. pp. 374–443. ISBN 978-0-306-46093-7.29.Jump up ^ Truong, Kevin; Ikura, Mitsuhiko (2001). "The use of FRET imagingmicroscopy to detect protein–protein interactions and proteinconformational changes in vivo". Current Opinion in Structural Biology11(5): 573–8. doi:10.1016/S0959-440X(00)00249-9. PMID 11785758.30.Jump up ^ Pollok, B; Heim, R (1999). "Using GFP in FRET-basedapplications". Trends in Cell Biology9 (2): 57–60. doi:10.1016/S0962-8924(98)01434-2. PMID 10087619.31.Jump up ^ Sekar, R. B.; Periasamy, A (2003). "Fluorescence resonance energytransfer (FRET) microscopy imaging of live cell protein localizations". The Journal of Cell Biology160 (5): 629–33. doi:10.1083/jcb.200210140.PMC 2173363. PMID 12615908.32.Jump up ^ Ni, Qiang; Zhang, Jin (2010). "Dynamic Visualization of CellularSignaling". In Endo, Isao; Nagamune, Teruyuki. Nano/Micro Biotechnology.Advances in Biochemical Engineering/Biotechnology, Volume 119. Springer.pp. 79–97. Bibcode:2010nmb..book...79N. doi:10.1007/10_2008_48.ISBN 978-3-642-14946-7. PMID 19499207.33.Jump up ^ Silvius, John R.; Nabi, Ivan Robert (2006). "Fluorescence-quenching and resonance energy transfer studies of lipid microdomains in model and biological membranes (Review)". Molecular Membrane Biology23(1): 5–16. doi:10.1080/09687860500473002. PMID 16611577.34.Jump up ^ S. A., Hussain et al. (2015). "Fluorescence Resonance EnergyTransfer (FRET) sensor" (PDF). J. Spectrosc. Dyn.5 (7): 1–16.35.Jump up ^ Hu, Chang-Deng; Chinenov, Yurii; Kerppola, Tom K. (2002)."Visualization of Interactions among bZIP and Rel Family Proteins in Living Cells Using Bimolecular Fluorescence Complementation". Molecular Cell9(4): 789–98. doi:10.1016/S1097-2765(02)00496-3. PMID 11983170。

-基础研.*基于TCGA数据库的肾嫌色细胞癌生物信息学分析瞿根义，王佳威，徐勇，阳光，聂海波，黄文琳，汤乘(中南大学湘雅医学院附属株洲医院泌尿外科，湖南株洲412007)Bioinformatic analysis of genes related to chromophobe renal cell carcinoma based onTCGAdatabaseQU Genyi,WANG Jiawei,XU Yong,YANG Guang,NIE Haibo,HUANG Wenlin,TANG Cheng (Department of Urology,Zhuzhou Hospital Affiliated to Xiangya School of Medicine,CSU,Zhuzhou412007,China)ABSTRACT:Objective To explore the Hub genes related to renal chromophobe cell carcinoma(chRCC)based on TCGA database.Methods Dataset of chRCC was extracted and screened from TCGA database.The differentially expressed genes (DEGs)were analyzed with edgeR algorithm of R software.After that,the volcano map was drawn.The DAVID and STRING onlinebioinforma+ic+oolswereused+oanalyzeandcons+ruc++hepro+ein-pro+einin+eracion(PPI)ne+work.Hubgeneswere screened with Cytoscape software,and survival analysis was carried out.Results A total of1850DEGs were screened out,in­cluding760up-regulated genes and1090down-regulated genes.GO enrichment analysis,KEGG pathway enrichment analysis and PPI network analysis showed the top10Hub genes were KNG1,AGT,CASR,SST,AGTR2,PMCH,GNG4,DRD2,MCHR2 and SSTR3.Survival analysis revealed that only MCHR2was significantly correlated with overall survival(OS)of chRCC(P# 0.05).Conclusion Based on the TCGA dat a base,mining of the10Hub genes of chRCC will help t o unders t a nd the genesis anddevelopmen.MCHR2maybecomeapo enial herapeuic arge andprognos ic marker of chRCC.KEY WORDS:chromophobe renal cell carcinoma；TCGA dat a base；bioinformat i cs；differen t i a lly expressed genes；Hub genes摘要：/的基于TCGA数据库肾嫌色细胞癌基因表达谱数据，探讨肾嫌色细胞癌发生的关键基因。

JDF-2S/N 0005473 & UPILLUSTRATED PARTS CATALOG Designs, materials, weights, specifications, and dimensions for equipment or replacement parts are subject to change without notice.BUNN-O-MATIC CORPORATION POST OFFICE BOX 3227SPRINGFIELD, ILLINOIS 62708-3227PHONE: (217) 529-6601 FAX: (217) 529-664438311.0000E 03/08 ©2005 Bunn-O-Matic Corporation DISCONTINUED VERSION Parts listed in this catalog may no longer be available.BUNN-O-MATIC COMMERCIAL PRODUCT WARRANTYBunn-O-Matic Corp. 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Accordingly, statements by such individuals, whether oral or written, do not constitute warranties and should not be relied upon.If BUNN determines in its sole discretion that the equipment does not conform to the warranty, BUNN, at its exclusive option while the equipment is under warranty, shall either 1) provide at no charge replacement parts and/or labor (during the applicable parts and labor warranty periods specified above) to repair the defective components, provided that this repair is done by a BUNN Authorized Service Representative; or 2) shall replace the equipment or refund the purchase price for the equipment.THE BUYER’S REMEDY AGAINST BUNN FOR THE BREACH OF ANY OBLIGATION ARISING OUT OF THE SALE OF THIS EQUIPMENT, WHETHER DERIVED FROM WARRANTY OR OTHERWISE, SHALL BE LIMITED, AT BUNN’S SOLE OPTION AS SPECIFIED HEREIN, TO REPAIR, REPLACEMENT OR REFUND.In no event shall BUNN be liable for any other damage or loss, including, but not limited to, lost profits, lost sales, loss of use of equipment, claims of Buyer’s customers, cost of capital, cost of down time, cost of substitute equipment, facilities or services, or any other special, incidental or consequential damages.BrewWISE, BUNN Gourmet Ice, BUNN Pour-O-Matic, BUNN, Bunn-OMatic, Bunn-O-Matic, BUNNlink, BUNNserve, BUNN Espress, DBC, Dr. Brew, Dual, EasyClear, EasyGard, Easy Pour, FlavorGard, Gourmet Ice, Gourmet Juice, High Intensity, IMIX, Infusion Series, Legendary for Quality, The Mark of Quality in Beverage Equipment Worldwide, My Café, Power-Logic, Safety-Fresh, Scale-Pro, Single, Smart Funnel, Smart Hopper, Soft Heat, SplashGard, System III, ThermoFresh, 392, AXIOM, Beverage Profit Calculator, Beverage Bar Creator, BrewLOGIC, BrewMETER, BrewWIZARD, BUNNSERVE, BUNNsource, Coffee At Its Best, Cool Froth, Digital Brewer Control, Intellisteam, Nothing Brews Like a BUNN, Pouring Profits, Pulse Wave, Quality Beverage Equipment Worldwide, Signature Series, Silver Series, Smart Heat, SmartWAVE, Tea At Its Best, The Horizontal Red Line, Titan, Ultra, are either trademarks or registered trademarks of Bunn-O-Matic Corporation.238311 020608TABLE OF CONTENTSMain Frame & Covers (4)Upper Cabinet & Panels (6)Refrigeration Components (8)Compressor Components (10)Fan Assembly (12)Water Tank Assy (14)Circulation Pump & Lines (18)Product Coil & Lines (20)Dispense Platform Assy (22)Dispense Valve Assy (24)Plain Water Manifold (Early Models) (26)Plain Water Manifold (Late Models) (28)Rinse Water Manifold & Solenoid (Early Models) (30)Rinse Water Manifold & Solenoid (Late Models) (34)Pump Assy (38)Bottle Adapter Assy (40)Door Assy (42)Electrical Components (46)Refillable Container Assy (50)Numerical Index (52)338311 111706438311 050807ITEM PART NO. QTY. DESCRIPTION5* Indicates the part number listed is for reference only. See DESCRIPTION for possible service replacement.MAIN FRAME & COVERS1 34409.0000 1 Base Frame Assy2 26528.0000 4 Leg 03996.0000 4 Pad, Non-skid3 34410.0000 1 Frame, Refridgeration - - - - - - - -4 Screw #10-32 x4 39428.0000* 2Bracket, Rear support (Late models with Water Inlet Valve)34797.0000* 2 Bracket, Rear support (Early models prior to Water Inlet Valve) 02332.0001 8 Screw, Crimptite #6 x .25"5 38469.0001* 1 Bracket, Upper Frame6 36192.1000 1 Panel w/Decals, Right (Includes item 11) 02332.0001 3 Screw, Crimptite #6 x .25"7 34786.1000 1 Panel w/Decals, Top (Includes item 8) 02332.0001 4 Screw, Crimptite #6 x .25"8 33458.0000 1 Decal, Refridgeration ON - OFF9 36191.1000 1 Panel w/Decals, Left (Includes items 10, 11, 12 & 24) 02332.0001 3 Screw, Crimptite #6 x .25"10 33461.0001 1 Decal, Refridgerant Charge11 27442.0000 2 Decal, Warning - Moving Parts12 00986.0002 1 Decal, Warning - Electrical13 39561.0001 1 Panel, Rear 02332.0001 4 Screw, Crimptite #6 x .25"14 33645.0000 1 Decal, Main Water Connection15 33646.0000 1 Decal, Rinse Water Connection (Early Models)16 00656.0000 1 Decal, Comply To Plumbing Code17 27354.0000 4 Bushing18 00668.0000 3 Plug19 35998.0001 1 Panel, Splash 01317.0003 2 Screw, Truss Head, Locking #8-32 x .38"20 21436.0000 2 Catch, Magnetic21 33538.0000*1 Bracket, Right33539.0000* 1 Bracket, Left 01311.0001 4 Screw, Truss Head, Locking #8-32 x .25"22 38765.1000 1 Drip Tray Assy (Includes strike plate and item 23)23 26801.0000 1 Grate, Drip Tray24 35277.0000 1 Schematic, Electrical38311 121207638311 111706ITEM PART NO. QTY. DESCRIPTION7* Indicates the part number listed is for reference only. See DESCRIPTION for possible service replacement.UPPER CABINET & PANELS1 35478.0000* 1 Cabinet (Not serviced) 24544.0000 10 Screw, Pan Head #8-182 34738.0000 1 Plate, Cabinet Mounting 01311.0001 4 Screw, Truss Head #8-32 x .25" Lkg.3 34784.0000 1 Plate, Bottom Closure 01359.0000 3 Screw, Flat Head #8-32 x .38"4 34362.0000 1 Bushing, Lower Hinge5 34790.0000 1 Wrap 01311.0001 4 Screw, Truss Head #8-32 x .25" Lkg.6 34785.0000* 1 Bracket, Hinge 01327.0000 3 Screw, Pan Head #10 -32 x .38"7 34666.0001 1 Catch, Door Latch 01382.0000 2 Screw, Truss Head #6 x .375"8 27394.0000 2 Plug9 00669.0002 4 Plug10 34419.0000 1 Coil, Water 24544.0000 4 Screw, Pan Head #8-1811 27246.0000 1 Sensor Assy, Temperature12 33669.0000 1 Clip, Adhesive Back13 33297.0000 1 Fan Assy. 03290.0000 1 Cable Tie 38616.0000 4 Screw, Pan Head #8-1814 34421.0000* 1 Cover, Fan 24544.0000 4 Screw, Hex Head w/Washer #10-2415 34422.1000 1 Shelf, Product (Includes items 16 & 17)16 33459.0003 2 Decal, Dispense/Rinse17 32461.0000 2 Gasket, Drip18 32455.0000 2 Knob19 27394.0000 2 Plug20 35930.0000 2 Nozzle21 32848.0000 2 O-ring22 35108.1000 2 Mixer, Spiral23 38519.0000 1 Decal, Dispense Ratio24 M2568.1000 - Grease, USDA H-1 (Use when assembling items 21 & 22)38311 121207838311 062905ITEM PART NO. QTY. DESCRIPTION9* Indicates the part number listed is for reference only. See DESCRIPTION for possible service replacement. 1 35164.1000 1 Air Filter2 29951.0001 1 Condenser 02335.0001 4 Screw, Crimptite #8 x .38"3 35180.0001 1 Tube (Requires brazing to install) (Not serviced)4 35386.0001 1 Tube (Requires brazing to install) (Not serviced)5 00396.0002 1 Tee (Requires brazing to install) (Not serviced)6 35385.0001 1 Tube (Requires brazing to install) (Not serviced)7 35580.0001 1 Tube (Requires brazing to install) (Not serviced)8 35175.1000 1 Filter Dryer Assy (Requires brazing to install)9 35179.0001 1 Tube (Requires brazing to install) (Not serviced)10 32776.0000 1 Accumulator11 35581.0001 1 Tube (Requires brazing to install) (Not serviced)12 - - - - - - - - 1 Compressor (See COMPRESSOR COMPONENTS) 34529.0000* 4 Washer 00901.0000 4 Nut .25"-2013 - - - - - - - - 4 Grommet14 - - - - - - - - 4 Sleeve 15 33536.0000 1 Platform 01317.0003 4 Screw, Truss Head, Locking #8-32 x .38"16 - - - - - - - - 1 Fan Assy (See FAN ASSEMBLY) 27243.0000 3 Screw #8-36 x .437"17 34418.0000* 1 Bracket 01317.0003 4 Screw, Truss Head, Locking #8-32 x .38"18 28567.0000 1 Mounting Kit (Includes four each of items 13 & 14)19 M2332.1000 - Insulation, Tube 25/50 - 1.6"ID x .50" wall x 6.0 Ft (Use as required) 20 M2336.1000 - Insulation, Tube 25/50 - 0.25"ID x .19" wall x 6.0 Ft (Use as required)REFRIGERATION COMPONENTSOrder item 1838311 0303081038311 111706ITEMPART NO.QTY.DESCRIPTION11* Indicates the part number listed is for reference only. See DESCRIPTION for possible service replacement.COMPRESSOR COMPONENTS- 38231.1000 1 Compressor 120V (Includes items 1 - 5) 33522.1001 1 Compressor 230V (Includes items 1 - 5) 1 38897.0000 1 Relay, Compressor 120V 38897.0001 1 Relay, Compressor 230V 2 38917.0000 1 Overload Protector 3 38916.0000 1 Retainer 4 38918.0000 1 Clip 538896.00001Cover38311 1117061238311 062905ITEMPART NO.QTY.DESCRIPTION13* Indicates the part number listed is for reference only. See DESCRIPTION for possible service replacement.FAN ASSEMBLY- 32204.1001 1 Fan Assy 120V (Includes items 1-4) 32204.1002 1 Fan Assy 230V (Includes items 1-4) 1 33603.0000 1 Motor Assy 120V 33603.0001 1 Motor Assy 230V 2 33602.0000 1 Blade, Fan 3 27258.0000 1 Silencer 423700.00001Nut .25"-2038311 0629051438311 111706ITEMPART NO.QTY.DESCRIPTION15* Indicates the part number listed is for reference only. See DESCRIPTION for possible service replacement.WATER TANK ASSY1 35165.0000 1 Tank Assy - - - - - - - - 4 Screw2 35272.1001 1 Lid Assy 24544.0000 6 Screw, Pan Head #8-183 35526.0000* 2 Bracket 02332.0001 2 Screw, Crimptite #6 x .25" 01317.0003 2 Screw, Truss Head, Locking #8-32 x .38"4 34403.1001 1 Panel Assy 00973.0000 4 Nut, Keps #6-325 33801.0000 1 Grommet6 29327.0000 1 Probe, Temperature & Dry Plug7 33801.0002 1 Grommet 7 02536.0000 1 Grommet9 02645.1000 1 Probe, Liquid Level 01522.0000 1 Washer 20203.0100 1 Lockwasher, Internal-tooth #800908.0000 1 Nut #8-32 10 21275.0004 3 Clamp .54" - .64"11 20976.0062* 1 Tube, Silicone .38" ID x 1.75" (Order item 35) 1221275.0005 1 Clamp .62" - .73" 120V 21275.0009 1 Clamp .65" - .78" 230V 13 35077.0000 1 Tee .38" Sngl. Barb 14 21275.0003 4 Clamp .57" - .70"15 34325.0003* 1 Hose, Flex .38" ID x .625" OD x 3.0" (Order item 36) 16 33623.0000 2 Elbow .38" Barb17 34325.0006* 1 Hose, Flex .38" ID x .625" OD x 2.25" (Order item 36) 18 20976.0016* 1 Tube, Silicone .38" ID x 2.75" (Order item 35) 19 33068.0000 1 Fitting .38" Barb x .25" Barb20 37809.1000 1 Pump Assy 120V (Includes items 11 - 19) 37265.0000 1 Pump Assy 230V 21 35132.0000* 1 Product Coil22 34416.0000 2 Spacer 00973.0000 4 Nut, Keps #6-32 23 03290.0000 2 Cable Tie 2432734.00016Cable Tie, 28.0"(Continued)38311 0508071638311 111706ITEMPART NO.QTY.DESCRIPTION17* Indicates the part number listed is for reference only. See DESCRIPTION for possible service replacement.WATER TANK ASSY25 33533.0000* 4 Spacer 26 33519.0000* 1 Evaporator Coil 27 36448.0000 2 Spacer 28 03290.0000 2 Cable Tie29 33670.0004* 1 Tube, Clear .25" x 8.0" (Not serviced) 30 38605.0000 1 Tee31 21275.0006 1 Clamp .40" - .50" 32 35174.0000 1 Fitting 33 33149.0000 1 Gasket 34 00976.0000 1 Cap35 35106.0000 1 Manifold, Return Water36 20976.1000 1 Tube, Silicone .38" ID x 12.0" (Cut to required length) 20976.1001 1 Tube, Silicone .38" ID x 36.0" (Cut to required length) 20976.1002 1 Tube, Silicone .38" ID x 120.0" (Cut to required length) 37 34325.1000 1 Hose, Flex .38" ID x .625" OD x 12.0" (Cut to required length) 34325.1001 1 Hose, Flex .38" ID x .625" OD x 36.0" (Cut to required length)34325.10021Hose, Flex .38" ID x .625" OD x 120.0" (Cut to required length)38311 1117061838311 062905ITEMPART NO.QTY.DESCRIPTION19* Indicates the part number listed is for reference only. See DESCRIPTION for possible service replacement.1 - - - - - - - - 1 Coil, Upper Cabinet (See UPPER CABINET & PANELS)2 34325.0004* 2 Tube, Silicone .38" ID x 1.5" (Order item 10)3 21275.0003 8 Clamp .57" - .70"4 37850.0000 2 Elbow .38" Barb5 34325.0010* 1 Tube, Silicone .38" ID x 17.0" (Order item 10)6 34325.0021* 1 Tube, Silicone .38" ID x 8.0" (Order item 10)7 35173.0001 1 Fitting .312" Fl x .375" Barb8 35305.0000 1 Gasket9 - - - - - - - - 1 Manifold, Return Water (See WATER TANK ASSEMBLY) 10 34325.1000 - Tube, Silicone .38" ID x 12.0" (Cut to required length) 34325.1001 - Tube, Silicone .38" ID x 36.0" (Cut to required length) 34325.1002 - Tube, Silicone .38" ID x 120.0" (Cut to required length) 11M2337.1000-Tube Insulation (Cut to required length)CIRCULATION PUMP & LINES38311 1117062038311 111706ITEMPART NO.QTY.DESCRIPTION21* Indicates the part number listed is for reference only. See DESCRIPTION for possible service replacement.PRODUCT COIL & LINES1 - - - - - - - - 1 Product Coil (See WATER TANK ASSEMBLY)2 35305.0000 2 Gasket3 35173.0001 1 Fitting .312" Fl x .375" Barb4 21275.0003 8 Clamp .57" - .70"5 34325.0010* 1 Hose, Flex .38" ID x 17.0" (Order item 19)(Early models prior to Water Inlet Valve) 34325.0014* 1 Hose, Flex .38" ID x 15.0" (Order item 19)(Late models with Water Inlet Valve)6 33151.0004 1 Fitting, Elbow .38" Barb7 33149.0000 1 Gasket8 12757.0000 1 Nut9 01514.0000 1 Lockwasher 10 12756.0000 1 Fitting, Bulkhead11 35172.0001 1 Fitting, Elbow .312" Fl x .375" Barb 12 34325.0004* 1 Hose, Flex .38" ID x 1.5" (Order item 19) 13 37850.0000 1 Elbow .38" Barb14 34325.0012* 1 Hose, Flex .38" ID x 10.5" (Order item 19) 15 35171.0000 1 Valve, Shut Off 16 34540.0000 1 Decal, Caution - Close17 34325.0004* 1 Hose, Flex .38" ID x 1.5" (Order item 19) 18 34462.0000 1 Coupling, Quick Disconnect, Male19 34325.1000 1 Hose, Flex .38" ID x 12.0" (Cut to required length) 34325.1001 1 Hose, Flex .38" ID x 36.0" (Cut to required length) 34325.1002 1 Hose, Flex .38" ID x 120.0" (Cut to required length)20M2337.1000-Insulation, Tube 25/50 - 0.5"ID x .19" wall x 6.0 Ft (Use as required)38311 111706Early Models2238311 110107ITEMPART NO.QTY.DESCRIPTION23* Indicates the part number listed is for reference only. See DESCRIPTION for possible service replacement.DISPENSE PLATFORM ASSEMBLY1 38116.1000 1 Platform Assy (Includes items2 - 18) 2 - - - - - - - - 2 Pump Assy (See PUMP ASSY) 01338.0000 2 Screw, Truss Head #8-32 x .625" 01522.0000 4 Washer00991.0000 4 Nut, Elastic Lock #8-32 3 32957.0001 8 Bushing, Rubber 4 21275.0006 4 Clamp .40" - .50"5 38018.1000 2 Dispense Valve (See DISPENSE VALVE) 01308.0000 4 Screw, Truss Head #6-32 x 25"6 37786.0001 2 Plate 01308.0000 2Screw, Truss Head #6-32 x 25"7 - - - - - - - - 1 Manifold, Plain Water (See PLAIN WATER MANIFOLD) 8 - - - - - - - - 1 Manifold, Rinse Water (See RINSE WATER MANIFOLD) 9 32756.1000 2 Bottle Adapter (See BOTTLE ADAPTER) 01308.0000 4 Screw, Truss Head #6-32 x 25" 10 38122.0000* 1 Plate 01311.0001 4Screw, Truss Head #8-32 x .25" Lkg.11 32458.0000 2 Clip, Retainer 12 38104.0000 2 Thumbscrew 13 32454.0000 2 Fitting 1434087.00002O-ringThe following items are not illustrated 15 35189.0000 1 Harness, Dispense Platform 16 35181.0001 1 Harness, Dispense Valve 17 - - - - - - - - 4 Cable Tie (Not serviced) 1812422.00002Clamp38311 1101072438311 062905ITEMPART NO.QTY.DESCRIPTION25* Indicates the part number listed is for reference only. See DESCRIPTION for possible service replacement.DISPENSE VALVE ASSEMBLY- 38018.1000 1 Dispense Valve Assy (Includes items 1 - 24) 1 37801.0001 1 Body 2 37990.0000 1 Seal 3 37843.0000 1 Nozzle, Water4 32252.0000 1 Valve, Solenoid (Includes items5 - 10) 5 33554.0000 1 Solenoid Valve Coil6 28481.0000 1 Guide Kit (Includes Guide, Washer and O-ring)7 33585.0000 1 Solenoid Valve Base Kit (Includes O-ring and items 6 & 7) 8 01111.0002 1 Solenoid Valve Repair Kit (Includes Plunger, Spring and O-ring) 9 33580.0000 1 O-ring 10 33553.0000 1 O-ring11 37987.0000 1 Bracket 01308.0000 2 Screw #6-32 x .25" 12 32463.0000 1 Fitting, Elbow13 32849.0001 1 O-ring (Also use item 24) 14 37853.0000 1 Clamp 01308.0000 1 Screw #6-32 x .25" 15 32603.0000 1 Sleeve16 32852.0000 1 O-ring (Also use item 24) 17 32602.0000 1 Piston18 32853.0001 1 Spring, Flow Control 19 32624.0000 1 Bonnet, Adjuster 20 33725.0000 1 O-ring (Also use item 24) 21 32623.0000 1 Adjuster, Position 22 32851.0000 1 O-ring (Also use item 24) 23 37977.0000 1 Bracket, Water Adjustment 01308.0000 2 Screw #6-32 x .25"24M2568.1000-Grease, USDA H-1 (Use when assembling items 13,16,20 & 22)38311 1117062638311 111706ITEMPART NO.QTY.DESCRIPTION27* Indicates the part number listed is for reference only. See DESCRIPTION for possible service replacement.PLAIN WATER MANIFOLD (Early Models)1 32631.00002 Fitting, Straight 2 32849.0001 2 O-ring3 38129.0005* 2 Hose, Braided .25" ID x 2.25" (Order item 12)4 21275.0006 4 Clamp .40 - .50"5 36204.0000* 1 Manifold, Plain SST6 34325.0017* 1 Hose, Flex .38" ID x 1.25" (Order item 13)7 21275.0003 2 Clamp .57 - .70"8 34463.0000 1 Coupling, Quick Disconnect, Female12 38129.1000 1 Hose, Braided .25" ID x 12.0" (Cut to required length) 38129.1001 1 Hose, Braided .25" ID x 36.0" (Cut to required length) 38129.1002 1 Hose, Braided .25" ID x 120.0" (Cut to required length) 13 34325.1000 1 Hose, Flex .38" ID x 12.0" (Cut to required length) 34325.1001 1 Hose, Flex .38" ID x 36.0" (Cut to required length)34325.10021Hose, Flex .38" ID x 120.0" (Cut to required length)38311 1117062838311 111706ITEMPART NO.QTY.DESCRIPTION29* Indicates the part number listed is for reference only. See DESCRIPTION for possible service replacement.PLAIN WATER MANIFOLD (Late Models)38311 1117061 32631.00002 Fitting, Straight 2 32849.0001 2 O-ring3 38129.0012* 2 Hose, Braided .25" ID x 3.0" (Order item 8)4 21275.0006 6 Clamp .40 - .50"5 38605.0000 1 Tee .25" Barb6 38129.0013* 1 Hose, Braided .25" ID x 5.25" (Order item 8)7 34461.0000 1 Coupling, Quick Disconnect, Female8 38129.1000 1 Hose, Braided .25" ID x 12.0" (Cut to required length) 38129.1001 1 Hose, Braided .25" ID x 36.0" (Cut to required length)38129.10021Hose, Braided .25" ID x 120.0" (Cut to required length)3038311 111706RINSE WATER MANIFOLD & SOLENOID (Early Models) ITEM PART NO. QTY. DESCRIPTION1 32756.10002 Bottle adapter (See BOTTLE ADAPTER)2 38129.0001*3 Hose, Braided .25" ID x .44" OD x 1.50" (Order item 37)3 21275.0006 16 Clamp .40" - .50"4 36203.0000* 1 Manifold. Rinse SST5 34460.0000 1 Fitting, Quick Disconnect, Male6 34461.0000 1 Fitting, Quick Disconnect, Female7 32745.0021* 1 Hose .25" ID x .44" OD x 15.75" (Order item 38)8 33151.0005 3 Fitting, Elbow .25" Barb9 33149.0001 4 Gasket10 01085.0002 1 Solenoid Valve 120V (Includes items 11 - 14)28005.0000 1 Solenoid Valve 230V (Includes items 11- 13)01327.0000 2 Screw, Pan Head #10-32 x .38" 120V02308.0000 2 Screw, Pan Head #8-32 x .38" 230V11 28480.0000 1 Coil, Solenoid 120V28020.0000 1 Coil, Solenoid 230V12 01111.0002 1 Solenoid Repair Kit (Includes Plunger, Spring and O-ring)13 28479.0000 1 Solenoid Base Kit 120V (Includes O-ring)28021.0000 1 Core Assy Kit 230V14 28481.0000 1 Guide Kit15 00402.0001 2 Connector16 00425.0001 1 Nipple17 00425.0002 1 Nut18 38129.0001* 1 Hose, Braided .25" ID x .44" OD x 1.50" (Order item 37)19 00459.0000 1 Fitting, Bulkhead01532.0000 1 Lockwasher, Internal-toth .438"00463.0000 1 Nut, Hex .375"-2020 38129.0009* 1 Hose, Braided .25" ID x .44" OD x 4.25" (Order item 37)21 33149.0000 3 Gasket22 33151.0004 1 Fitting, Elbow .38" Barb23 21275.0003 4 Clamp .57" - .70"24 34325.0004* 1 Hose, Flex .38" ID x .595" OD x 1.5" (Order item 39)25 32749.0001 1 Tee .38" Barb x .38" Barb x .25" Barb(Continued)RINSE WATER MANIFOLD & SOLENOID (Early Models) ITEM PART NO. QTY. DESCRIPTION26 34325.0006* 1 Hose, Flex .38" ID x .595" OD x 2.25" (Order item 39)27 33150.0000 1 Nut28 33686.0000 1 Nipple29 23720.0000 1 Strainer Assy (Includes items 30 - 32)30 - - - - - - - - 1 Housing, Strainer (Order item 29)31 23721.0000 1 Screen32 23723.0000 1 End Cap33 21275.0006 2 Clamp .41 - .50"34 35573.0000* 1 Bracket, Rinse Solenoid (Early Models)01317.0003 2 Screw, Truss Head #8-32 x .38" Lkg35 32463.0000 2 Fitting, Elbow36 32849.0001 2 O-ring37 38129.1000 - Hose, Braided .25" ID x .44" OD x 12.0" (Cut to required length)38129.1001 - Hose, Braided .25" ID x .44" OD x 36.0" (Cut to required length)38129.1002 - Hose, Braided .25" ID x .44" OD x 120.0" (Cut to required length)38 32745.1002 - Hose .25" ID x .44" OD x 120.0" (Cut to required length)39 34325.1000 - Hose, Flex .38" ID x .595" OD x 12.0" (Cut to required length)34325.1001 - Hose, Flex .38" ID x .595" OD x 36.0" (Cut to required length)34325.1002 - Hose, Flex .38" ID x .595" OD x 120.0" (Cut to required length)ITEMPART NO.QTY.DESCRIPTIONRINSE WATER MANIFOLD & SOLENOID (Late Models)(Continued)1 32756.10002 Bottle adapter (See BOTTLE ADAPTER) 2 32849.0001 2 O-ring3 32463.0000 2 Fitting, Elbow4 38129.0009* 1 Hose, Braided .25" ID x 4.25" (Order item 40)5 21275.0006 8 Clamp .40" - .50"6 38129.0004* 1 Hose, Braided .25" ID x 3.50" (Order item 40)7 38605.0000 1 Tee .25" Barb8 38129.0001* 1 Hose, Braided .25" ID x 1.50" (Order item 40) 9 34460.0000 1 Fitting, Quick Disconnect, Male 10 34461.0000 1 Fitting, Quick Disconnect, Female11 32745.0021* 1 Hose .25" ID x 15.75" (Order item 39)(Early models prior to Water Inlet Valve) 38129.0014* 1 Hose .25" ID x 13.0" (Order item 40)(Late models with Water Inlet Valve) 12 01085.0002 1 Solenoid Valve 120V (Includes items 13 - 16) 28005.0000 1 Solenoid Valve 230V (Includes items 13- 15) 01327.0000 2 Screw, Pan Head #10-32 x .38" 120V02308.0000 2 Screw, Pan Head #8-32 x .38" 230V 1328480.0000 1 Coil, Solenoid 120V 28020.0000 1 Coil, Solenoid 230V14 01111.0002 1 Solenoid Repair Kit (Includes Plunger, Spring and O-ring) 15 28479.0000 1 Solenoid Base Kit 120V (Includes O-ring) 28021.0000 1 Core Assy Kit 230V 16 28481.0000 1 Guide Kit17 35573.0000* 1 Bracket, Rinse Solenoid (Early Models) 01317.0003 2 Screw, Truss Head #8-32 x .38" Lkg 18 00402.0001 1 Connector19 33151.0005 1 Fitting, Elbow .25" Barb 20 33149.0001 1 Gasket21 33152.0001 1 Fitting .38" Barb x .125" MPT22 34325.0022* 1 Hose, Flex .38" ID x 2.50" (Order item 41) 23 32750.0001 1 Tee .38" Single Barb24 34325.0021* 1 Hose, Flex .38" ID x 8.0" (Order item 41) 25 33686.0000 1 Nipple33150.00001NutRINSE WATER MANIFOLD & SOLENOID (Late Models) ITEM PART NO. QTY. DESCRIPTION26 21275.0003 5 Clamp .57" - .70"27 36203.0000* 1 Manifold. Rinse SST (Early Models)28 33149.0000 2 Gasket29 23720.0000 1 Strainer Assy (Includes items 30 - 32)30 - - - - - - - - 1 Housing, Strainer (Order item 29)31 23721.0000 1 Screen32 23723.0000 1 End Cap33 01533.0005 2 Spacer01326.0006 2 Screw, Truss Head #10-32 x .625"34 00464.0001 1 Elbow .25" Barb x .125"MPT35 34325.0004* 2 Hose, Flex .38" ID x 1.50" (Order item 41)36 35077.0000 1 Tee, Nylon .38" Barb37 36233.0000 1 Valve, Water Inlet 120V36233.0001 1 Valve, Water Inlet 230V32283.0000 2 Screw, Pan Head M4 x 6mm38 37297.0000 1 Elbow39 32745.1002 - Hose .25" ID x .44" OD x 120.0" (Cut to required length)40 38129.1000 - Hose, Braided .25" ID x .44" OD x 12.0" (Cut to required length)38129.1001 - Hose, Braided .25" ID x .44" OD x 36.0" (Cut to required length)38129.1002 - Hose, Braided .25" ID x .44" OD x 120.0" (Cut to required length) 41 34325.1000 - Hose, Flex .38" ID x .595" OD x 12.0" (Cut to required length)34325.1001 - Hose, Flex .38" ID x .595" OD x 36.0" (Cut to required length)34325.1002 - Hose, Flex .38" ID x .595" OD x 120.0" (Cut to required length)PUMP ASSEMBLYITEM PART NO. QTY. DESCRIPTION- 33803.1001 1 Pump Assy (Includes items 1 - 29)1 32613.1002 1 Motor Assy01322.0001 4 Screw #8-32 x 1.25"2 32654.0000 1 Magnet Hub01512.0000 1 Lockwasher, Internal-tooth #1001331.0004 1 Screw, Locking #10-32 x .50"3 33608.1000 1 Sensor, RPM02336.0000 2 Screw #4-40 x .38"4 07130.0000* 1 Cable Tie5 32957.0000 4 Bushing6 37152.0001 1 Mount Assy, Pump7 29904.0000 1 Insert, Well Nut #8-328 33781.0000 1 Gasket9 28755.0010* 1 Tube, Norprene .25" ID x 18.5" lg (Order item 17)10 32732.0000 1 Clamp .39"/.48"11 32656.0000 1 Fitting, Elbow .25" Barb x .38" Barb12 21275.0003 1 Clamp .57"/.70"13 34325.0019* 1 Hose, Flex .375" ID x 5.75" lg (Order item 18)14 28759.0000 1 Pump Assy. (Includes items 15 & 16)15 28794.0000 1 Rotor16 01370.0001 4 Screw, Pan Head #8-32 x 2.45"17 28755.1000 - Tube, Neoprene .25" ID x .45" OD x 12.0" (Use as required)28755.1001 - Tube, Neoprene .25" ID x .45" OD x 36.0" (Use as required)28755.1002 - Tube, Neoprene .25" ID x .45" OD x 60.0" (Use as required) 18 34325.1000 - Hose, Flex .375" ID x 12.0" (Use as required)34325.1001 - Hose, Flex .375" ID x 36.0" (Use as required)34325.1002 - Hose, Flex .375" ID x 120.0" (Use as required)19 39689.0000 - Pump Tube (Includes items 9,10,11,12 & 13)。

规范信息
本产品符合所有适用的欧盟指令要求。您可以从网站 /compliance 找到完整的符合性声明。
此符号表示不得将此产品作为生活垃圾丢弃，应将其送到合适的 回收站点进行回收。正确处理和回收有助于保护自然资源、人类 健康和环境。想要获得更多关于此产品的处理和回收的信息，请 联系当地市政当局、垃圾处理服务部门或您购买此产品的商店。
中国危险物质限用表
有毒或有害物质或元素名称及成分
有毒或有害物质和元素
零件名称
铅
汞
(Pb) (Hg)
镉 ()
六价铬 (CR(VI))
多溴化 联苯 (PBB)
印刷电路板 X
O
O
O
O
金属零件
X
O
O
O
O
塑料零件
O
O
O
O
O
扬声器
X
O
O
O
O
线缆
X
O
O
O
O
此表格依据 SJ/T 11364 的要求制定。
O：表示此零件中所有均质材料所包含的此类有毒或有害物质均低于 GB/T 26572 中的限定要求。
6. 只能使用制造商指定的附件/配件。
7.
只能使用制造商指定或随本设备一起销售的推车、支架、
三角架、托架或工作台。如果使用推车，则在移动推车/设
备时应格外小心，以免因倾倒而造成伤害。
警告/小心：
包含可能导致窒息危险的小部件。不适合 3 岁以下的儿童使用。
本产品含有磁性材料。有关这是否会影响到您的植入式医疗器 械，请咨询您的医生。
包装清单................................................................................................................................................................................................... 5 可选配件................................................................................................................................................................................................... 5 检查和维护....................................................................................................................................................................................................... 6 保持防风雨性........................................................................................................................................................................................... 6 推荐使用的工具.............................................................................................................................................................................................. 6 产品尺寸........................................................................................................................................................................................................... 7 ArenaMatch Utility AMU105........................................................................................................................................................... 7 ArenaMatch Utility AMU108........................................................................................................................................................... 8 ArenaMatch Utility AMU206.......................................................................................................................................................... 9 ArenaMatch Utility AMU208........................................................................................................................................................ 10