IDH-305_HNMR_27269_MedChemExpress

D5319dat Rev 07/231Product InformationDeoxyribonuclease I bovineRecombinant, expressed in Pichia pastoris , buffered aqueous glycerol solution, ≥ 5,000 units/mg proteinD5319Product DescriptionCAS Registry Number: 9003-98-9Enzyme Commission (EC) Number: 3.1.21.1 Synonyms: DNase I,Deoxyribonucleate 5'-Oligonucleotidohydrolase Molecular mass: ~39 kDaExtinction Coefficient: E 2801% = 11.1Deoxyribonuclease I (DNase I) is an endonuclease that acts on phosphodiester bonds adjacent topyrimidines to produce polynucleotides with terminal 5'-phosphates. A tetranucleotide is the smallest average digestion product. DNase I hydrolyzes single-stranded and double-stranded DNA.•In the presence of Mg 2+ ions, DNase I attacks each strand of DNA independently and the cleavage sites are random.•If Mn 2+ ions are present, both DNA strands are cleaved at approximately the same site.1When chromatin DNA is digested, the reaction rate is restricted by the association of DNA with histones.1 DNase I is found in most cells and tissues. Inmammals, the pancreas is one of the best sources for the enzyme. Pancreatic DNase I was the first isolated DNase.DNase I can be used to remove DNA from protein and nucleic acid samples, and to nick DNA as a first step to incorporate labeled bases into DNA.This recombinant bovine DNase I is a glycoprotein, produced without the addition of any animal-derived materials. Several theses 2 and dissertations 3-7 have cited use of product D5319 in their protocols.ActivatorsDNase I has an absolute requirement for divalent metal cations:• Mg 2+ is the most commonly used divalent cation.8,9•Mn 2+, Ca 2+, Co 2+, and Zn 2+ will also activate DNase I.8-10A concentration of 5 mM Ca 2+ will stabilize DNase I against proteolytic digestion. 0.1 mM Ca 2+ is needed to reduce the rate of inactivation by one-half.11Inhibitors•2-Mercaptoethanol (the reduced enzyme is inactive, but can be reactivated in the presence of Ca 2+ or Mg 2+ ions)10 • Chelators (such as EDTA) • Sodium dodecyl sulfate (SDS)12 •Actin 13There is no single general inhibitor specific for DNase I.2 Citrate inhibits Mg 2+-activated DNase I, but not Mn 2+-activated DNase I.Optimal pHThe optimal pH of DNase I activity is dependent on the divalent ion present. In the presence of both Mg 2+ and Ca 2+, the optimal pH is between 7-8, while in the absence of Ca 2+, the optimal pH is between 5.5-6.0.14Precautions and DisclaimerThis product is for R&D use only. Not for drug,household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.The life science business of Merck operates as MilliporeSigma in the U.S. and Canada.Merck and Sigma-Aldrich are trademarks of Merck KGaA, Darmstadt, Germany or its affiliates. All other trademarks are the property of their respective owners. Detailed information on trademarks is available via publicly accessible resources.© 2015-2023 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved. D5319dat Rev 07/23 DT,GCY,LS,RBG,RC,MAM2ProductThis product is supplied as a solution in 4 mg/mL glycine (pH 5.0), 5 mM calcium acetate, and 50% glycerol.Specific activity: ≥ 5,000 units/mg proteinUnit definition: One unit will produce a change in A 260 of 0.001 per minute per mL at pH 5.0 at 25 °C using DNA, Type I or III, as the substrate. This enzyme assay reaction is performed in 83 mM acetate buffer (pH 5.0), at 25 °C, containing 4.2 mM Mg 2+, in a 3 mL reaction.ImpuritiesProtease: None Detected RNase: None detectedStorage/StabilityThis product retains activity for at least two years when stored at –20 °C.References1. Sambrook, J. et al ., Molecular Cloning: ALaboratory Manual , 2nd edition. Cold Spring Harbor Laboratory Press (Cold Spring Harbor, New York, USA), Vol. 2, p. 5.83 (1989). 2. Schöftner, Leonie Christine, "Development of ahuman 3D skin model to study cutaneous T cells". University of Salzburg, M.Sc. thesis, pp. 18, 21 (2021). 3. Alsiwiehri, Naif, "Identification andcharacterisation of novel cancer testis antigens in human cancer cells". Bangor University, Ph.D. dissertation, p. 35 (2017). 4. Deans, Jonathan Robert, "The Role of NuclearReceptors in Tissue-Specific Gene Expression: The Impact of Genetic Variation on DNA Binding". University of California Riverside, Ph.D. dissertation, p. 34 (2017). 5. Hempel, Heidi Anne, "The role of mast cells inprostate cancer initiation and recurrence". Johns Hopkins University, Ph.D. dissertation, p. 55 (2017). 6. Wu, Zhiyuan, "Immunophenotyping ofColorectal Cancer Reveals a Reduction of CD73 Expression on Tumor-Infiltrating Lymphocytes". Friedrich-Alexander-Universität Erlangen-Nürnberg, Dr. med. dissertation, p. 9 (2021).7. Hinterdobler, Julia Michaela, "Linkinginflammation and atherosclerosis – novel mechanistic insights into how vascular inflammation fuels progression ofatherosclerosis". Technischen UniversitätMünchen, Dr. rer. nat. dissertation, p. 31 (2022). 8. Weir, A. F., Methods Mol. Biol ., 16, 1-6 (1993). 9. Weir, A. F., Methods Mol. Biol ., 16, 7-16 (1993). 10. Price, P. A. et al ., J. Biol. Chem ., 244(3),929-932 (1969). 11. Price, P. A., et al ., J. Biol Chem ., 244(3),917-923 (1969). 12. Liao, T.-H., J. Biol. Chem ., 250(10), 3831-3836(1975). 13. Love, J. D., and Hewitt, R. R., J. Biol. Chem .,254(24), 12588-12594 (1979). 14. Lazarides, E., and Lindberg, U., Proc. Natl. Acad.Sci. USA , 71(12), 4742-4746 (1974).NoticeWe provide information and advice to our customers on application technologies and regulatory matters to the best of our knowledge and ability, but without obligation or liability. Existing laws and regulations are to be observed in all cases by our customers. This also applies in respect to any rights of third parties. Our information and advice do not relieve ourcustomers of their own responsibility for checking the suitability of our products for the envisaged purpose. The information in this document is subject to change without notice and should not be construed as acommitment by the manufacturing or selling entity, or an affiliate. We assume no responsibility for any errors that may appear in this document.Technical AssistanceVisit the tech service page at /techservice .Terms and Conditions of UseWarranty, use restrictions, and other conditions of sale may be found at /terms .Contact InformationFor the location of the office nearest you, go to /offices .。

网络出版时间：２０２３－０５－０９１６：５４：４７　网络出版地址：ｈｔｔｐｓ：／／ｋｎｓ．ｃｎｋｉ．ｎｅｔ／ｋｃｍｓ／ｄｅｔａｉｌ／３４．１０８６．Ｒ．２０２３０５０８．１４３０．０３８．ｈｔｍｌ◇抗炎免疫药理学◇ＭｉｃｒｏＲＮＡ ２０４／ ２１１敲除加重ＤＭＭ小鼠骨关节炎滑膜增生和滑膜炎症滕　辉，陈斯佳，王婷玉（上海交通大学医学院附属第九人民医院药剂科，上海　２０００１１）收稿日期：２０２３－０１－１０，修回日期：２０２３－０３－１０基金项目：国家自然科学基金资助项目（Ｎｏ８１８７４０１１，８２１７２３８３）作者简介：滕　辉（１９９６－），女，硕士生，研究方向：药理学，Ｅ ｍａｉｌ：ｈｔｅｎｇ＿ｙｘｙ＠１６３．ｃｏｍ；王婷玉（１９８２－），女，博士，主任药师，博士生导师，研究方向：药理学、临床药学，通信作者，Ｅ ｍａｉｌ：ｄｒｔｙｗａｎｇ＠１６３．ｃｏｍｄｏｉ：１０．１２３６０／ＣＰＢ２０２２１２０２５文献标志码：Ａ文章编号：１００１－１９７８（２０２３）０５－０９２６－０６中国图书分类号：Ｒ ３３２；Ｒ３２２ ７２；Ｒ３６４ ５；Ｒ３９４ ２；Ｒ６８４ ３摘要：目的　观察ｍｉｃｒｏＲＮＡ ２０４／ ２１１敲除对内侧半月板失稳术（ｄｅｓｔａｂｉｌｉｚａｔｉｏｎｏｆｔｈｅｍｅｄｉａｌｍｅｎｉｓｃｕｓ，ＤＭＭ）所致骨关节炎（ｏｓｔｅｏａｒｔｈｒｉｔｉｓ，ＯＡ）小鼠的影响。

方法　使用１２只Ｃ５７ＢＬ／６Ｊ（ＷＴ）小鼠和１２只ｍｉｃｒｏＲＮＡ ２０４／ ２１１基因敲除（ｍｉＲ ２０４／ ２１１ ｄＫＯ）小鼠，ＷＴ小鼠随机分为假手术组和ＤＭＭ组，即ＷＴ ｃｏｎｔｒｏｌ组和ＷＴ＋ＤＭＭ组；ｍｉＲ ２０４／ ２１１ ｄＫＯ小鼠随机分为假手术组和ＤＭＭ组，即ｄＫＯ组和ｄＫＯ＋ＤＭＭ组。

ＷＴ＋ＤＭＭ组和ｄＫＯ＋ＤＭＭ组行ＤＭＭ。

ＶｏｎＦｒｅｙ法测定小鼠对疼痛的敏感性。

３个月后取材，膝关节行微计算机断层扫描（Ｍｉｃｒｏ ＣＴ）检测、组织染色以及免疫组化分析，ＲＴ ｑＰＣＲ检测ＤＲＧ组织中相关疼痛和炎症因子。

专利名称：一种顺式乌头酸酐键阿霉素/人血清白蛋白（CAD/HSA）组合物及其制备方法和应用专利类型：发明专利
发明人：李红玉,王加涛,余兰,邹玥,乔志伟,李洋
申请号：CN202111555576.0
申请日：20211217
公开号：CN114569736A
公开日：
20220603
专利内容由知识产权出版社提供
摘要：本发明涉及一种叶酸/顺式乌头酸酐键阿霉素/人血清白蛋白(FA/CAD/HSA)组合物及其制备方法和应用。

该组合物是由顺式乌头酸酐键阿霉素和人血清白蛋白通过化学偶联而成的靶向给药系统，具有pH敏感释药功能和一定的肿瘤靶向性，能够在杀伤肿瘤细胞的同时降低阿霉素的毒性，有益于临床应用。

申请人：兰州大学
地址：730000 甘肃省兰州市城关区天水南路222号兰州大学
国籍：CN
更多信息请下载全文后查看。

NAD-苹果酸脱氢酶（紫外分光光度法货号：BC1040规格：50T/48S产品组成：使用前请认真核对试剂体积与瓶内体积是否一致，有疑问请及时联系索莱宝工作人员。

试剂名称规格保存条件提取液液体50 mL×1瓶4℃保存试剂一液体40 mL×1瓶4℃保存试剂二粉剂×2支-20℃保存试剂三粉剂×2支-20℃保存溶液的配制：1、试剂二：临用前加入360 μL双蒸水，用不完的试剂仍-20℃保存；2、试剂三：临用前加入327 μL双蒸水，用不完的试剂仍-20℃保存。

产品说明：MDH（EC 1.1.1.37）广泛存在于动物、植物、微生物和培养细胞中，线粒体中MDH是TCA循环的关键酶之一，催化苹果酸形成草酰乙酸；相反，胞浆中MDH催化草酰乙酸形成苹果酸。

草酰乙酸是重要的中间产物，连接多条重要的代谢途径。

因此，MDH在细胞多种生理活动中扮演着重要的角色，包括线粒体的能量代谢、苹果酸-天冬氨酸穿梭系统、活性氧代谢和抗病性等。

根据不同的辅酶特异性，MDH分为NAD-依赖的MDH和NADP-依赖的MDH，细菌中通常只含有NAD-MDH，在真核细胞中，NAD-MDH分布于细胞质和线粒体中。

NAD-MDH催化NADH还原草酰乙酸生成苹果酸，导致340nm处光吸收下降。

注意：实验之前建议选择2-3个预期差异大的样本做预实验。

如果样本吸光值不在测量范围内建议稀释或者增加样本量进行检测。

需自备的仪器和用品：紫外分光光度计、台式离心机、水浴锅、可调式移液器、1mL石英比色皿、研钵/匀浆器、蒸馏水。

操作步骤：一、样本处理（可适当调整待测样本量，具体比例可以参考文献）细菌或培养细胞：收集细菌或细胞到离心管内，离心弃上清，按照每200万细菌或细胞加入400μL提取液，超声波破碎细菌或细胞（功率20%，超声3s，间隔10s，重复30次），8000g 4℃离心10min，取上清，置冰上待测。

组织：称取约0.05g组织，加入1mL提取液进行冰浴匀浆；8000g 4℃离心10min，取上清，置冰上待测。