高效液相色谱法测定人血浆中伏立康唑含量

高效液相色谱法测定注射用伏立康唑的含量
王黎;李华
【期刊名称】《西北药学杂志》
【年(卷),期】2007(022)002
【摘要】目的建立 HPLC 测定注射用伏立康唑含量的方法.方法选用 VP-ODS
C18色谱柱(150 mm×4.6 mm,5μm),流动相为乙腈-水(40:60),柱温为30℃,检测
波长为255 nm.结果伏立康唑在0.101 0～0.960 5 g·L-1范围内质量浓度与峰面积呈良好线性关系(r=0.999 5),平均回收率为99.8%,RSD为0.27%.结论本法简便、快速、可靠,可用于注射用伏立康唑的质量检验.
【总页数】2页(P51-52)
【作者】王黎;李华
【作者单位】西北大学分析科学研究所,陕西,西安,710069;西安医学院药学系,陕西,西安,710068;西北大学分析科学研究所,陕西,西安,710069
【正文语种】中文
【中图分类】R927.2
【相关文献】
1.HPLC法测定注射用伏立康唑含量及有关物质 [J], 姚毅;唐燕平
2.高效液相色谱-荧光法或超高效液相色谱-四极杆/轨道阱质谱法测定抑郁症小鼠
海马中5-羟色胺和相关的2种物质含量的比较 [J], 王丽; 朱军; 环飞; 程洁; 吴倩3.钩藤药材中异钩藤碱含量的超高效液相色谱和高效液相色谱法测定 [J], 李思蒙;侴桂新
4.RP-HPLC法测定注射用伏立康唑含量及其有关物质 [J], 浦锡娟
5.高效液相色谱和超高效液相色谱法测定乳制品中三聚氰胺含量的对比 [J], 冯小丽
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•论著•中国当代医药2021年3月第28卷第9期超高效液相色谱-串联质谱法测定血浆屮伏立康唑的浓度及其在深部真菌感染患者伏立康唑血药浓度监测屮的应用葛欣刘妍妍刘平山东省滕州市中心人民医院药学部，山东滕州277500［摘要］目的采用超高效液相色谱-串联质谱法测定患者血浆中伏立康唑的浓度,并用于监测血药浓度。

方法采用超高效液相色谱-串联质谱法进行测定。

血浆样品以氟康唑为内标，经氢氧化钠碱化、乙酸乙酯萃取后进行分析。

采用Acquity UPLC®BEH-C I8色谱柱(2.1mmx50mm,1.7滋m)分离，流动相为水(含0.1%，甲酸及2mmol/L甲酸铵)-乙腈，梯度洗脱,流速为0.10mL/min，柱温为40益,进样量为1滋L,总分析时间为5.0min;离子源为电喷雾离子源，采用正离子电离模式(ESI+),扫描方式为多离子反应监测模式渊MRM)。

结果血浆中伏立康唑浓度的线性范围为10~10000ng/mL(Y=1.30393x10-3X+1.00217,r=0.9992),定量下限为10ng/mL；质控样品准确度为95.2% ~104.8%■曰日内、日间RSD均<7%；方法回收率〉95%(RSD<7%J,提取回收率〉75%(RSD<5%J。

采用该法检测12例深部真菌感染患者体内伏立康唑的血药浓度为817.29~9054.24ng/mL,有4例患者的血药浓度不在推荐治疗浓度范围内。

结论该方法快速、灵敏、准确，专属性强，重复性好，适用于伏立康唑治疗药物监测。

［关键词］伏立康唑;超高效液相色谱-串联质谱法;血药浓度；治疗药物监测；侵袭性真菌感染冲图分类号］R917.101［文献标识码］A［文章编号］1674-4721(2021)3(c)-0026-06Concentration determination of Voriconazole in patients plasma by ultra performance liquid chromatography-tandem mass spectrometry and its application in monitoring the plasma concentration of Voriconazole in patients with deep fungal infectionGE Xin LIU Yan-yan LIU PingDeparLmenL of Pharmacy,Tengzhou CenLral People's HospiLal,Shandong Province,Tengzhou277500,China[Abstract]Objective To develop an ulLra performance liquid chromaLography-Landem mass specLromeLry(UPLC-MS/MS) meLhod for deLerminaLion of Voriconazole in paLienLs plasma and apply iL Lo drug moniLoring.Methods UPLC-MS/MS meLhod was ing fluconazole as inLernal sLandard,Lhe plasma samples were alkalized wiLh sodium hydroxide and exLracLed wiLh eLhyl aceLaLe and analyzed.The separation was achieved on an AcquiLy UPLC®BEH-C^column(2.1mmx50mm,1.7滋m)and eluLed wiLh linear gradient using waLer(containing0.1%formic acid and2mmol/L am­monium formaLe)-aceLoniLrile aL flow raLe of0.10mL/min.The injection volume was1滋L and Lhe column LemperaLure was40益.The LoLal Lime of Lhe analysis was5.0min.DeLecLion of Lhe analyLes was achieved using positive ion elecLro-spray ionizaLion(ESI+)in Lhe mulLiple reacLion moniLoring(MRM)mode.Results The linear range of voriconazole was 10-10000ng/mL(Y=1.30393x10-3X+1.00217,r=0.9992),wiLh Lhe lower limiL of quanLiLaLion of10ng/mL.The accu­racy of qualiLy conLrol sample was95.2%—104.8%・.InLra-day and inLer-day relative standard deviations were boLh< 7%.MeLhod recovery was>95%(RSD<7%)and exLracLion recovery was>75%(RSD<5%).The plasma concenLraLions of Voriconazole in12paLienLs wiLh invasive fungal infecLion deLermined by Lhis meLhod were817.29-9054.24ng/mL, and of Lhose4paLienLs'plasma concentrations were ouLside Lhe recommended LreaLmenL concentration range.Conclu­sion The esLablished meLhod is rapid,sensiLive,accuraLe,specific and repeaLable,and can be applied for Lhe Lherapeu-Lic drug moniLoring of Voriconazole.［作者简介］葛欣(1984-),女，山东滕州人，博士，主管药师,研究方向:治疗药物监测[Key words]Voriconazole;UlLra performance liquid chro-maLography-Landem mass specLromeLry;Plasma concenLra-Lion;TherapeuLic drug moniLoring;Invasive fungal infecLion26CHINA MODERN MEDICINE Vol.28No.9March 2021中国当代医药2021年3月第28卷第9期•论著窑伏立康唑（Voriconazole）是新型第2代三唑类广谱抗真菌药,为氟康唑的衍生物，是治疗侵袭性真菌感染（invasive fungal infection,IFI）的一线药物［1］。

高效液相色谱法测定血浆中伏立康唑浓度的方法及应用程振宇;王焕锋【期刊名称】《现代诊断与治疗》【年(卷),期】2017(028)010【摘要】建立测定人血浆中伏立康唑浓度的高效液相色谱方法,分析临床应用效果,为个体化用药提供参考.35例患者静脉滴注伏立康唑150~300mg,q12h,监测伏立康唑谷浓度.色谱柱为COSNOSIL C18柱(4.6mm×250mm,5μm),流动相为乙腈:水(V:V=36:64),流速为1.0ml/min,柱温为40℃,检测波长为247nm.伏立康唑浓度在0.2~16.0μg/ml内呈良好的线性关系(r2=0.9994).伏立康唑批内、批间精密度分别为为6.2%~10.6%和5.9%~12.5%,提取回收率为(85.32±10.55)%~(91.27±7.01)%,所有样品在考察条件下稳定.我院所监测的35例重症患者伏立康唑血样中,有22例(62.9%)患者伏立康唑谷浓度在1.5~4.0μg/ml之间.本法用于重症患者伏立康唑血药浓度监测快速、准确、重现性好,可为优化治疗方案提供依据.【总页数】4页(P1835-1838)【作者】程振宇;王焕锋【作者单位】郑州市第一人民医院药剂科;中州大学,河南郑州 450001【正文语种】中文【中图分类】R927.2【相关文献】1.高效液相色谱法测定人血浆中伏立康唑浓度及其应用 [J], 刘亮;郭美华;陈岩;鲁静;钱钊;高春璐;海鑫2.HPLC-MS/MS法检测血浆伏立康唑、泊沙康唑和伊曲康唑浓度的方法建立及在白血病患者用药监测中的应用 [J], 王磊;孙文利;刘瑞;王红春;刘红星3.高效液相色谱法测定人血浆中米卡芬净浓度及其在重症患者中的应用 [J], 赵明明;陈亚南;路童;李国飞;肇丽梅4.超高效液相色谱-串联质谱法测定血浆中伏立康唑的浓度及其在深部真菌感染患者伏立康唑血药浓度监测中的应用 [J], 葛欣;刘妍妍;刘平5.高效液相色谱法测定家兔血浆中Z－47浓度及其在药代动力学研究中的应用 [J], 钟大放;陈仁弟;李雪庆;秦文杰;顾景凯因版权原因，仅展示原文概要，查看原文内容请购买。

.94.中国抗生素杂志2019年1月第44卷第1期文章编号：1001-8689(2019)01-0094-07 HPLC-MS/MS法测定人血浆中伏立康瞠浓度的不确定度评价于思源冯志平(江门市中心医院药学部，中山大学附属江门医院，江门529000)摘要：目的评价高效液相色谱-串联质谱法(HPLC-MS/MS)测定人血浆中伏立康哇浓度的不确定度。

方法分析HPLC-MS/MS法测定人血浆中伏立康哇浓度过程中的不确定度来源，计算其大小并合成。

结果HPLC-MS/MS法测定伏立康哇浓度的不确定度在低浓度时主要由标准曲线拟合、样品提取和重复性引入，在高浓度时主要由仪器允差，重复性和样品提取引入。

人血浆中伏立康哇在低浓度(0.025yg/mL)、中浓度(1.5pg/mL)和高浓度(8.0ng/mL)的扩展不确定度分别为0.0018,0.2057和0.8723 (置信概率P=95.45%,A=2)o结论为有效减小测量结果的不确定度，建议在今后工作中应尽量避免过宽的标准曲线范围，增加测定点的个数和重复测定次数，同时提高向处理后的基质中添加内标或质控溶液的加样技巧。

关键词：高效液相色谱-串联质谱法；伏立康哇；人血浆；浓度测定；不确定度中图分类号：R978文献标志码：AThe uncertainty evaluation of the determination of voriconazole concentrationsin human plasma by HPLC-MS/MSYu Si-yuan and Feng Zhi-ping(Department of Pharmacy,Jiangmen Central Hospital,Affiliated Jiangmen Hospital of Sun Yat-San University,Jiangmen529000)Abstract Objective To evaluate the uncertainty in the determination of voriconazole(VOR)concentrations in human plasma by HPLC-MS/MS.Methods The uncertainty sources in the determination of VOR were analyzed and the uncertainty was evaluated and combined.Results The uncertainty in the determination of VOR concentrations by HPLC-MS/MS is mainly caused by the calibration curve fitting,the extraction,and the repeatability at low concentrations. At high concentrations,the uncertainty was majorly caused by LC-MS/MS errors,the extraction,and the repeatability.The expanded uncertainty ofVOR at low(0.025pg/mL),middle(1.5pg/mL),and high(8.0|ig/mL)concentrations were0.001& 0.2057,and0.8723(P=95.45%,k=2),respectively.Conclusion In order to reduce the uncertainty of the measurement results effectively,it is suggested that the wide range of standard curves should be avoided,the number of points and the number of repeated measurements should also be increased,and then the addition of the internal standard or the quality control working solution to the treated matrix should be improved in the future work.Key words HPLC-MS/MS;Voriconazole;Human plasma;Concentration determination;Uncertainty不确定度的定义为表征合理地赋予被测量之值的分散性，广义而言，意为对测量结果正确性的可疑程度。

高效液相色谱法测定血浆中伏立康唑的浓度苏涌;杨春兰;黄燕;夏泉;许杜娟【摘要】Objective To develop a HPLC method for the determination of voriconazole in Plasma.Methods Hypersil ODS2 C18(4.6 mm×250 mm,5μm)column was used and the flow rate was 1.0 mL·min-1.The mobile phase was consisted of acetonitrile-water (36:64).The column temperature was 30 ℃ and the detective wave length was 254 nm.Estazolam was selected as internal standard.Results A linearity of voriconazole concentration curve was between 0.06-20 mg·L-1,r=0.999 9.The intra-day and inter-day RSDs were between 1.49%-2.91%,absolute recovery rates of voriconazole were between 87.07%-96.82%.By detecting plasma voriconazole concentration in 15 patients,the average plasma drug concentration was (4.99±4.77) mg·L-1.Conclusion This method was sensitive and specific,which is suitable for clinical therapeutic drug monitoring (TDM).%目的建立适用于病人血浆中伏立康唑浓度测定的高效液相色谱法.方法色谱柱为Hypersil ODS2 C18(4.6 mm×250 mm,5 μm);流动相为乙腈:水(36:64);流速为1.0 mL·min-1;检测波长为254 nm;柱温为30 ℃;内标为艾司唑仑.结果伏立康唑及内标艾司唑仑的色谱峰分离良好,伏立康唑在0.06~20 mg·L-1范围内线性关系良好,r=0.999 9.日内及日间RSD在1.49%~2.91%之间,绝对回收率在87.07%~96.82%之间.检测15例使用伏立康唑的病人血浆药物浓度,其均值(4.99±4.77) mg·L-1.结论该方法专属性强、灵敏度高,适合伏立康唑的血浆浓度测定.【期刊名称】《安徽医药》【年(卷),期】2017(021)002【总页数】4页(P244-247)【关键词】伏立康唑;治疗药物监测;高效液相色谱法【作者】苏涌;杨春兰;黄燕;夏泉;许杜娟【作者单位】安徽医科大学第一附属医院药剂科、国家中医药管理局中药化学三级实验室,安徽合肥 230022;安徽医科大学第一附属医院药剂科、国家中医药管理局中药化学三级实验室,安徽合肥 230022;安徽医科大学第一附属医院药剂科、国家中医药管理局中药化学三级实验室,安徽合肥 230022;安徽医科大学第一附属医院药剂科、国家中医药管理局中药化学三级实验室,安徽合肥 230022;安徽医科大学第一附属医院药剂科、国家中医药管理局中药化学三级实验室,安徽合肥 230022【正文语种】中文伏立康唑是广谱三氮唑类抗真菌药，该药具有良好的药代动力学特征：起效迅速，达峰时间(Tmax)为1～2 h；生物利用度高达96%；半衰期约为6 h[1]。

高效液相色谱检测血浆伏立康唑浓度研究雷玲【摘要】目的采用高效液相色谱法(HPLC)检测人体血浆中伏立康唑的浓度,为伏立康唑临床药物个体化应用提供依据.方法以乙酸乙酯-二氯甲烷(75:25)为萃取剂,用ZORBAX Extend-C18(4.6 mmx250 mm,5 μm)色谱柱,劳拉西泮为内标,在0.1 mol/L磷酸二氢钠-水-乙腈(20:35:45)、256 nm波长条件下对血浆样本中利康唑的浓度进行检测.方法学考察后,选取2013年1月至2013年12月在海军总医院感染科室中口服伏立康唑治疗(40例)及静脉注射伏立康唑治疗(20例)的真菌感染患者为受试对象,采用HPLC法检测患者血浆中伏立康唑的浓度.结果口服组各个时间点血浆中伏立康唑在色谱柱中保留时间为5.8～8.2(7.0±0.9) min,注射组各个时间点血浆中伏立康唑的保留时间为6.2～8.7(7.4±0.8)min.劳拉西泮保留时间为5.2～6.3(5.7±0.4)min.线性关系为Y =0.5238X+0.002 (r2 =0.9997),定量下限口服组为0.1 mg/L,静脉注射组为0.12 mg/L.0.2、2.0和8.0 mg/L 3个浓度的相对回收率分别为83.24％、84.06％和80.27％;日内、日间精密度的相对标准偏差RSD均＜5.0％.结论采用HPLC方法能够检测患者血浆中伏立康唑的浓度.经方法学考察发现该HPLC检测方法具有专属性强、灵敏度高等优点,并且本方法操作简单,分析结果准确,可为临床检测伏立康唑血浆浓度和药动学研究提供有利依据.【期刊名称】《医学综述》【年(卷),期】2014(020)019【总页数】3页(P3646-3648)【关键词】高效液相色谱;伏立康唑;浓度【作者】雷玲【作者单位】海军总医院药剂科,北京100048【正文语种】中文【中图分类】R917伏立康唑作为一种新型高效广谱抗菌药物，在临床抗真菌治疗方面发挥着重要作用。

反相高效液相色谱法测定人血浆中伏立康唑的浓度李宇;张蕾;邓明影;张永煌;陈卫东;史天陆【摘要】Objective To establish RP-HPLC method for the determination of voriconazole in human plasma.Methods Syncronis C18 column(5 μm,4.6 mm ×250 mm)was adopted.The mobile phase was 0.025 mol·L -1 potassium dihydrogen phosphate buffer solu-tion(pH =5.25,added with triethylamine 62.5 μL)and acetonitrile and methanol(35∶45∶20).The flow rate was 1.0 mL·min -1 , the detection wavelength was 260 nm,and the column temperature was 30℃.Ketoconazole was used as intern al standard,20 μL injected directly after plasma samples were extracted by acetonitrile.Results A linearity was obtained within the range of voriconazole concen-trations from 0.2 to 10 mg·L -1 .LLOQ was 0.2 mg·L -1 .The standard curve was As =0.460 9C +0.030 1(r2 =0.999 6,n =5). The intra-day and inter-day RSD were less than 7%.The accuracy of voriconazole was between 98% and 103%,and the extraction re-coveries of voriconazole was between 86% and 95%.The stable inspection RSD was less than 8%.Conclusion The method was sim-ple,highsensitive,accurate,and well stable for the determination of voriconazole in human plasma,which provided a basis for individu-alized voriconazole treatment.%目的：建立反相高效液相色谱法（Reversed Phase High Performance Liquid Chromatography，RP-HPLC）测定人血浆中伏立康唑的浓度。

人血浆中伏立康唑的HPLC测定黄成坷;周伶俐;邱相君;胡国新;王增寿【期刊名称】《中国抗生素杂志》【年(卷),期】2009(034)006【摘要】目的建立检测人血浆伏立康唑的高效液相色谱法,并对其在人体内的药代动力学进行研究.方法以ZORBAX Extend-C18(4.6 mm×250mm,5μm)为色谱柱;乙腈-0.15mol/L磷酸二氢钠-水(45∶20∶35)为流动相,流速0.9ml/min;以劳拉西泮为内标,血浆样品经乙酸乙酯和正己烷(4∶1)混合提取后,在256nm波长下进行检测.结果伏立康唑在25～1600ng/ml浓度范围内线性关系良好(r=0.9999),定量下限为25ng/ml;50、400和1200ng/ml 3种浓度的日间、日内精密度RSD(n=5)均小于10%;相对回收率均在85%～115%范围内,绝对回收率大于75%.单剂量口服200mg伏立康唑的主要药代动力学参数Tmax、Cmax、t1/2和AUC(0-t)分别为(1.70±0.41)h、(1037.01±81.18)ng/ml、(5.88±0.93)h和(6643.92±696.70)ng·h/ml.健康志愿者单剂口服伏立康唑200mg,其药动学参数与国外相关报道一致.结论本方法操作简便,结果准确;适用于血浆伏立康唑的检测.【总页数】4页(P359-362)【作者】黄成坷;周伶俐;邱相君;胡国新;王增寿【作者单位】温州医学院附属第二医院,温州,325027;温州医学院附属第二医院,温州,325027;河南科技大学医学院药理学教研室,洛阳,471003;温州医学院药理学教研室,温州,325027;温州医学院附属第二医院,温州,325027【正文语种】中文【中图分类】R978.5【相关文献】1.HPLC法测定人血浆中伏立康唑及其代谢物的浓度 [J], 王志君;杨云云;张文静;王学彬;宋洪杰;王卓2.HPLC-MS/MS法测定人血浆中伏立康唑浓度的不确定度评价 [J], 于思源;冯志平3.HPLC测定人血浆中伏立康唑的血药浓度 [J], 高元峰; 欧阳林旗; 肖望重; 欧阳荣4.HPLC-MS/MS法同时测定人血浆中伏立康唑及其氮氧化代谢物浓度 [J], 李天萍;王永庆;于勇;应玉雯;杨雨晴;谢利军;陈娟;张宏文;周辰;孙鲁宁5.HPLC法测定人血浆中伏立康唑的浓度 [J], 史长城;叶健;卓广超;楼江;李晴宇;林能明因版权原因，仅展示原文概要，查看原文内容请购买。

高效液相色谱法测定血浆中伏立康唑的浓度苏涌，杨春兰，黄燕，夏泉，许杜娟(安徽医科大学第一附属医院药剂科、国家中医药管理局中药化学三级实验室，安徽合肥230022)摘要：目的建立适用于病人血浆中伏立康唑浓度测定的高效液相色谱法。

方法色谱柱为Hypersll O D S2 C1S (4.6 m m X250 mm,5 |jun);流动相为乙腈:水（36:64);流速为1.0 m L • min4 ;检测波长为254 nm ;柱温为30 T ：;内标为艾司挫仑。

结果伏 立康唑及内标艾司唑仑的色谱峰分离良好，伏立康唑在0.06 ~20 m g • 1T1范围内线性关系良好，r=0. 999 9。

日内及日间 RSD 在1.49% ~2.91%之间,绝对回收率在87.07% ~96.82%之间。

检测15例使用伏立康唑的病人血浆药物浓度,其均值 (4.99 ±4.77) mg • 1T1。

结论该方法专属性强、灵敏度高，适合伏立康唑的血浆浓度测定。

关键词:伏立康唑;治疗药物监测;高效液相色谱法doi ： 10. 3969/j. issn. 1009 - 6469. 2017.02.013Determination of plasma concentration of voriconazole by HPLCSU Yong,YANG Chunlan,HUANG Yan,XIA Quan,XU Dujuan{Department of Pharmacy y The First Affiliated Hospital of Anhui Medical University ,Third-Grade PharmaceuticalChemistry Laboratory of State Administration of Traditional Chinese Medicine , Hefei , A nhui 230022, China )Abstract ：Objective To develop a HPLC method for the determination of voriconazole in Plasma. Methods Hypersil 0DS2 C18(4. 6mm x250 mm,5 jjim) column was used and the flow rate was 1. 0 mL • min 1. The mobile phase was consisted of acetonitrile-water (36-64). The column temperature was 30 and the detective wave length was 254 nm. Estazolam was selected as internal standard. Results A linearity of voriconazole concentration curve was between 0. 06-20 mg • L 1 ,r = 0. 999 9. The intra-day and inter-day RS- Ds were between 1.49% -2. 91 % ,absolute recovery rates of voriconazole were between 87. 07% -96. 82% . By detecting plasma voricon­azole concentration in 15 patients, the average plasma drug concentration was (4. 99 ± 4. 77 ) mg • L 1. Conclusion This method was sensitive and specific, which is suitable for clinical therapeutic drug monitoring ( TDM).Key words ：Voriconazole ；TDM；HPLC通信作者:许杜娟，女，主任药师，硕士生导师，研究方向：临床药学，肿瘤药理学，E-mail: xuduj+uan6365@ 163. com态血药浓度情况，为该药的后期临床疗效研究提供 参考依据和监测手段。

HPLC法测定人血浆中伏立康唑的浓度作者：史长城叶健卓广超楼江李晴宇林能明来源：《中国药房》2021年第20期中圖分类号 R927.2 文献标志码 A 文章编号 1001-0408（2021）20-2525-05DOI 10.6039/j.issn.1001-0408.2021.20.15摘要目的：建立测定人血浆中伏立康唑浓度的方法。

方法：血浆经乙腈沉淀蛋白后，以利鲁唑为内标，采用高效液相色谱（HPLC）法测定其中伏立康唑的血药浓度。

色谱柱为Agilent Zorbax Eclipse Plus C18，流动相为乙腈-0.01 mol/L磷酸二氢钾溶液（38 ∶ 62，V/V），流速为1.0 mL/min，柱温为40 ℃，检测波长为255 nm，进样量为20 μL。

同时将该方法应用于10例使用伏立康唑患者的血药浓度测定。

结果：伏立康唑检测质量浓度的线性范围为0.2～20.0 μg/mL（r=0.999 6），定量下限为0.2 μg/mL，日内、日间RSD均小于7%（n=6或n=18），准确度平均值为98.59%～106.18%，提取回收率平均值为86.77%～89.86%（RSD关键词伏立康唑;高效液相色谱法;血药浓度Determination of Voriconazole Concentration in Human Plasma by HPLCSHI Changcheng1，2，YE Jian3，ZHUO Guangchao4，LOU Jiang1，2，LI Qingyu2，LIN Nengming1，2（1. Zhejiang Provincial Key Laboratory of Clinical Cancer Pharmacology and Toxicology Research，the Affiliated Hangzhou First People’s Hospital， School of Medicine，Zhejiang University， Hangzhou 310006， China; 2. Dept. of Pharmacy， the Affiliated Hangzhou First People’s Hospital， School of Medicine， Zhejiang University， Hangzhou 310006， China; 3. Dept. of Respiratory Medicine，the Affiliated Hangzhou First People’s Hospital， School of Medicine， Zhejiang University， Hangzhou 310006， China; 4. Central Laboratory， the Affiliated Hangzhou First People’s Hospital， School of Medicine， Zhejiang University， Hangzhou 310006， China）ABSTRACT OBJECTIVE： To establish the method for the determination of voriconazole concentration in human plasma. METHODS： After protein precipitation with acetonitrile， using riluzole as internal standard， plasma concentration of voriconazole were determined by HPLC method. The determination was performed on Agilent Zorbax Eclipse Plus C18 column with mobile phase consisted of acetonitrile-0.01 mol/L monopotassium phosphate solution （38 ∶ 62， V/V） at the flow rate of 1.0 mL/min. The column temperature was maintained at 40 ℃， and the detection wavelength was 255 nm. The sample size was 20 μL. Meanwhi le， the method was applied to determine plasma concentration of voriconazole in 10 patients receiving voriconazole. RESULTS：The liner range of voriconazole was 0.2-20.0 μg/mL （r=0.999 6）. The lower limit of quantitation was 0.2 μg/mL. RSDs of intra-day and inter-day were both lower than 7% （n=6 or n=18）. Average accuracies were 98.59%-106.18%. Average extraction recoveries were 86.77%-89.86% （RSDKEYWORDS Voriconazole; HPLC; Plasma concentration伏立康唑是广谱三唑类抗真菌药物，对曲霉菌、念珠菌等常见致病菌和放线菌、镰刀菌等罕见真菌均具有良好的抗菌活性，临床应用广泛[1]。

超高效液相色谱法测定人体血清中伏立康唑药物浓度杨婷;陈璐璐;胡伟;朱熙;刘丽萍;杨雅茹【期刊名称】《中国现代医学杂志》【年(卷),期】2016(026)019【摘要】目的采用超高效液相色谱法测定人血清中伏立康唑(VRC)浓度,并用于临床上该药的血药浓度监测.方法以地西泮为内标,色谱柱为Agilent Poroshell 120 EC-C18(4.6×100 mm,2.7μm),流速1 ml/nin,流动相为乙腈-0.2％甲酸水溶液(45∶55),检测波长254 nm,进样量10μl,柱温40℃.结果 VRC及地西泮的保留时间分别为2.52和4.06 min;血清中VRC线性范围为0.12～12.0μg/ml(r =0.9999),定量下限为0.12μg/ml,日内精密度相对标准偏差(RSD)均＜1.9％,日间精密度RSD 均＜2.1％,低、中、高3个浓度方法回收率为98.39％、101.16％和96.63％.结论该方法用于VRC的血药浓度检测,具有操作简便、稳定性良好,节约时间等优点.【总页数】5页(P37-41)【作者】杨婷;陈璐璐;胡伟;朱熙;刘丽萍;杨雅茹【作者单位】安徽医科大学第二附属医院药剂科,安徽合肥230601;安徽医科大学第二附属医院药剂科,安徽合肥230601;安徽医科大学第二附属医院药剂科,安徽合肥230601;安徽医科大学第二附属医院药剂科,安徽合肥230601;安徽医科大学第二附属医院药剂科,安徽合肥230601;安徽医科大学第二附属医院药剂科,安徽合肥230601【正文语种】中文【中图分类】R969.1【相关文献】1.LC-MS/MS法同时测定干血斑中伏立康唑及其主要代谢产物氮氧化伏立康唑的质量浓度 [J], 程晓亮; 邸莹; 张春; 李娜; 李嘉璐; 张雷; 姚鸿萍2.超高效液相色谱法测定人血清中伏立康唑血药浓度与临床应用 [J], 陈君;黄春新;王敏3.超高效液相色谱-串联质谱法测定血浆中伏立康唑的浓度及其在深部真菌感染患者伏立康唑血药浓度监测中的应用 [J], 葛欣;刘妍妍;刘平4.超滤法测定伏立康唑的结合型药物浓度及血浆蛋白结合率 [J], 孟冬梅;刘冬冬;喻鹏久;魏理5.超高效液相色谱法测定人血浆中伏立康唑浓度 [J], 庄奕筠;张吟;许秋霞;高雅莉因版权原因，仅展示原文概要，查看原文内容请购买。

高效液相色谱法测定人血浆中伏立康唑浓度及其应用刘亮;郭美华;陈岩;鲁静;钱钊;高春璐;海鑫【摘要】目的:建立一种简单、准确的测定人血浆中伏立康唑浓度的方法,并应用于临床伏立康唑血药浓度监测,以促进个体化用药.方法:采用高效液相色谱法,以酮康唑为内标.色谱柱:Waters Symmetry C18柱(4.6 mm×250 mm,5μm),流动相为乙腈-0.01 mol·L-1醋酸铵溶液(50:50),流速1.0 mL·min-1,紫外检测波长255 nm,柱温40℃,进样量40μL.结果:伏立康唑在0.1~20.0μg·mL-1范围内线性良好,r=0.9997.定量下限为0.1μg·mL-1.伏立康唑高、中、低三个浓度的提取回收率分别是96.24%~101.61%、89.06%~93.55%、83.76%~108.87%.日内精密度RSD<9.44%,日间精密度RSD<5.56%.本方法测定患者的伏立康唑血浆药物浓度分别为6.36、4.98、0.54μg·mL-1.结论:该方法灵敏度高,操作简便,结果准确,适用于伏立康唑血药浓度监测.%Objective: To establish a simple and accurate method for the determination of voriconazole in human plasma. This method was used to monitor voriconazole blood concentration and promote the individualized medication. Methods: With ketoconazole as the internal standard, the chromatographic column was the Waters Symmetry C18 (4.6 mm×250 mm, 5 μm), the mobile phase was acetonitrile-0.01 mol·L-1 ammonium acetate solution (50 : 50). The detection wavelength was 255 nm with the flow rate of 1.0 mL·min-1, and the column te mperature was 40 ℃ . The injection volume was 40 μL. Results: The concentration of voriconazole in the range of 0.1 – 20.0μg·mL-1 (r = 0.9997) showed the good linearity. The lower limit of quantitation was 0.1 μg·mL-1. The recovery rates of low, middle and highconcentrations were 96.24% – 101.61%, 89.06% – 93.55% and 83.76% –108.87%, respectively. The intra-day RSDs were less than 9.44% and the inter-day RSDs were less than 5.56%. The concentration of voriconazole in patient plasma were 6.36, 4.98, 0.54 μg·mL-1, respectively by the method. Conclusion: The method was sensitive, simple, accurate and applicable for voriconazole blood concentration monitoring.【期刊名称】《中国药物应用与监测》【年(卷),期】2017(014)004【总页数】4页(P205-208)【关键词】伏立康唑;血药浓度监测;高效液相色谱法【作者】刘亮;郭美华;陈岩;鲁静;钱钊;高春璐;海鑫【作者单位】哈尔滨医科大学附属第一医院,黑龙江哈尔滨 150001;哈尔滨医科大学附属第一医院,黑龙江哈尔滨 150001;哈尔滨医科大学附属第一医院,黑龙江哈尔滨 150001;哈尔滨医科大学附属第一医院,黑龙江哈尔滨 150001;哈尔滨医科大学附属第一医院,黑龙江哈尔滨 150001;哈尔滨医科大学附属第一医院,黑龙江哈尔滨 150001;哈尔滨医科大学附属第一医院,黑龙江哈尔滨 150001【正文语种】中文【中图分类】R917伏立康唑（voriconazole，VRC）是第二代三唑类抗真菌药物，该药在氟康唑的基础上引入甲基，并以氟代嘧啶环取代了氟康唑的三唑环，通过抑制真菌细胞膜麦角固醇的合成，从而抑制真菌的生长[1-2]。