下载文档原格式
DOI：10.16662/ki.1674-0742.2024.01.102探讨重组人脑利钠肽治疗慢性心衰急性加重期患者的临床效果周纪星临沂市中心医院心内科，山东临沂276000［摘要］目的分析慢性心衰急性加重期患者采用重组人脑利钠肽治疗的临床效果。
方法随机选取2020年6月—2021年6月临沂市中心医院心内科收治的60例慢性心衰急性加重期患者为研究对象。
按照区组随机法分为对照组和研究组，各30例，对照组患者开展常规治疗，研究组患者开展常规治疗+重组人脑利钠肽治疗，分析重组人脑利钠肽的治疗价值。
结果治疗后，研究组患者红细胞沉降率、红细胞压积、血浆动力黏度、全血动力黏度、血液血浆动力黏度、QT间期离散度水平、冠状动脉左前降支内径、左室舒张末期内径、左室收缩末期内径水平更低，左室射血分数水平更高，差异有统计学意义（P均<0.05）；治疗后，研究组患者血肌酐（77.31±6.43）µmol/L、脑钠肽前体（1 328.43±150.96）pg/mL水平更低，表皮生长因子受体（55.31±5.29）mL/（min·1.73 m2）水平更高，差异有统计学意义（t=6.243、5.228、6.351，P均<0.05）。
结论重组人脑利钠肽治疗在改善患者的心衰相关指标、心功能和血流流变学指标方面表现更为优越，患者治疗后临床效果更好。
［关键词］慢性心衰急性加重期；重组人脑利钠肽；心功能［中图分类号］R541.4 ［文献标识码］A ［文章编号］1674-0742（2024）01(a)-0102-04Exploring the Clinical Effect of Recombinant Human Brain Natriuretic Peptide in Treating Patients with Acute Exacerbation of Chronic Heart FailureZHOU JixingDepartment of Cardiology, Linyi Central Hospital, Linyi, Shandong Province, 276000 China[Abstract] Objective To analyze the clinical effect of recombinant human brain natriuretic peptide treatment in pa⁃tients with acute exacerbation of chronic heart failure. Methods 60 patients with acute exacerbation of chronic heart failure admitted to the cardiology Department of Linyi Central Hospital from June 2020 to June 2021 were randomly selected as the study objects. According to the block randomized method, the patients were divided into control group and study group, with 30 cases in each group. The patients in the control group received conventional treatment, and the patients in the study group received conventional treatment plus recombinant human brain natriuretic peptide therapy. The therapeutic value of recombinant human brain natriuretic peptide was analyzed. Results After treatment, erythrocyte sedimentation rate, hematocrit, plasma dynamic viscosity, whole blood dynamic viscosity, blood plasma dy⁃namic viscosity, QT interval dispersion level, left anterior descending coronary artery diameter, left ventricular end-diastolic diameter and left ventricular end-systolic diameter were lower in the study group, and left ventricular ejec⁃tion fraction was higher, the differences were statistically significant (all P<0.05). After treatment, the levels of serum creatinine (77.31±6.43) µmol/L, brain natriuretic peptide precursor (1 328.43±150.96) pg/mL were lower in the study group, and the levels of epidermal growth factor receptor (55.31±5.29) mL/ (min·1.73 m2) were higher, the differences were statistically significant (t=6.243, 5.228, 6.351, all P<0.05). Conclusion Recombinant human brain natriuretic peptide treatment was more superior in improving patients' heart failure-related indexes, cardiac function and blood flow rheology indexes, and patients had better clinical outcomes after treatment.[作者简介] 周纪星（1974-），男，硕士，副主任医师，研究方向为冠心病的基础与临床研究。
西红花提取物调控免疫细胞，提高程序性死亡受体-1抑制剂治疗肺腺癌效果的实验研究作者：李诗颖李存雅张雪钟薏来源：《上海医药》2024年第01期摘要目的：多项研究提示，西红花提取物能影响肿瘤的发展进程。
本实验探究西红花提取物在肺腺癌小鼠模型中对肿瘤免疫微环境和免疫治疗的影响，为西红花提取物抗肿瘤研究提供更多基础性数据。
方法：构建Lewis肺癌细胞和萤光素酶稳定结合的小鼠皮下瘤模型，观察西红花提取物对小鼠皮下瘤和肿瘤免疫微环境的影响：运用活体成像技术跟踪肿瘤生长情况；运用流式细胞技术检测小鼠CD4+、CD8+ T细胞的数量及占比；运用反转录-聚合酶链式反应技术检测程序性死亡受体配体1、含有T细胞免疫球蛋白和黏蛋白结构域的蛋白3（T cell immunoglobulin and mucin domaincontaining protein 3， TIM3）、淋巴细胞活化基因-3（lymphocyte-activation gene-3， LAG3）、具有免疫球蛋白和ITIM结构域的T细胞免疫受体（T cell immunoreceptor with immunoglobulin and ITIM domain， TIGIT）、胸腺细胞选择相关的高迁移率族蛋白（thymocyte selection-associated high mobility group box， TOX）1、TOX2、TOX3基因的mRNA表达情况。
结果：与对照组相比，给予西红花提取物能一定程度地抑制小鼠皮下瘤的生长（P关键词西红花免疫微环境肺腺癌免疫治疗中图分类号：R965； R282.71 文献标志码：A 文章编号：1006-1533（2024）01-0003-09引用本文李诗颖，李存雅，张雪，等. 西红花提取物调控免疫细胞，提高程序性死亡受体-1抑制剂治疗肺腺癌效果的实验研究[J]. 上海医药， 2024， 45（1）： 3-11； 28.基金项目：上海市2022年度“科技创新行动计划”医学创新研究专项项目（22Y31920104）；上海市虹口区第二轮“国医强优”三年行动计划（2022—2024年）中西医结合重点专科、薄弱专科建设项目（HKGYQYXM-2022-10）；上海市2021年度“科技创新行动计划”扬帆计划项目（21YF444400）；上海市2022年度“科技创新行动计划”启明星培育（扬帆专项）项目（22YF1444900）；山东省乡村振兴基金会张秀兰慈善基金项目Experimental study of saffron extracts to modulate immune cells to improve the efficacy of a programmed death-1 inhibitor in the treatment of lung adenocarcinomaLI Shiying1， LI Cunya1， ZHANG Xue2， ZHONG Yi1（1. Department of Oncology， Shanghai TCM-Integrated Hospital， Shanghai University of Traditional Chinese Medicine， Shanghai 200082， China； 2. Shanghai Traditional Chinese Medicine Co.， Ltd.， Shanghai 200082， China）ABSTRACT Objective： A number of studies have shown that saffron extracts can affect the development of tumor. This study explored the effect of saffron extract on tumor immune microenvironment and immunotherapy in a mouse model of lung adenocarcinoma so as to provide more basic data for the anti-tumor research of saffron extracts. Methods： The transplanted tumor model of Lewis lung carcinoma-luciferase in mice was established to detect the effect of saffron extracts on the transplanted tumor in vivo. At the same time， the tumor growth was tracked by in vivo imaging technique. The number and proportion of CD4+ and CD8+ T cells were determined by flow cytometry. The mRNA levels of programmed death-ligand 1， T cell immunoglobulin and mucin domain-containing protein 3 （TIM3）， lymphocyte-activation gene-3 （LAG3）， T cell immunoreceptor with immunoglobulin and ITIM domain （TIGIT）， thymocyte selection-associated high mobility group box （TOX） 1， TOX2 and TOX3 were detected by reverse transcription-polymerase chain reaction （RT-PCR） and immunohistochemical techniques to verify the effect of saffron extracts on the regulation of tumor immune microenvironment. Results：Compared with the control group， the administration of saffron extracts could inhibit the growth of subcutaneous tumor in mice to a certain extent， and the number and proportion of CD4+ and CD8+ T cells were increased （PKEY WORDS saffron； immune microenvironment； lung adenocarcinoma； immunotherapy肿瘤是一类恶性疾病，2018年全球肿瘤死亡病例数达约960万人，较2008年增加26.3%，其中男性肿瘤死亡病例数增加最多的是肺癌，增加了23.4万人[1-2]。
Journal of China Pharmaceutical University 2023，54（2）：141 - 149学 报通用型CAR-T细胞药物的开发策略及临床研究进展王辰，张正平，李盈淳*（正大天晴药业集团股份有限公司研究院，南京 211100）摘 要 嵌合抗原受体T细胞（chimeric antigen receptor T-cell，CAR-T）免疫疗法已在多种血液肿瘤的临床治疗中取得了突破性进展。
然而，目前国内外获批上市的CAR-T细胞产品均属于自体CAR-T。
相比于自体CAR-T治疗，通用型CAR-T优势显著，可满足更多患者的治疗需求，但同时也具有较高的技术壁垒。
本文围绕通用型CAR-T进行综述，明确指出通用型CAR-T发展面临的两大挑战，并从引起两大难题的机制出发总结分析可行的解决策略，同时对国内外通用型CAR-T布局企业及其优势产品的最新临床进展进行综述，从另一方面探讨开发策略的可行性，以期为开发新一代通用型CAR-T细胞治疗产品提供思路。
关键词CAR-T；同种异体；免疫排斥；基因编辑；临床研究；进展中图分类号R730.51 文献标志码 A 文章编号1000 -5048（2023）02 -0141 -09doi：10.11665/j.issn.1000 -5048.20211125001引用本文王辰，张正平，李盈淳.通用型CAR-T细胞药物的开发策略及临床研究进展［J］.中国药科大学学报，2023，54（2）：141–149. Cite this article as：WANG Chen，ZHANG Zhengping，LI Yinchun. Development strategy and clinical research progress of universal chime‑ric antigen receptor T-cell drugs［J］.J China Pharm Univ，2023，54（2）：141–149.Development strategy and clinical research progress of universal chimeric antigen receptor T-cell drugsWANG Chen, ZHANG Zhengping, LI Yinchun*Chia-Tai Tianqing Pharmaceutical Group Co., Ltd. Research Institute, Nanjing 211100, ChinaAbstract Chimeric antigen receptor T-cell (CAR-T) immunotherapy has made a breakthrough in the clinical treatment of a variety of hematological tumors.However, the CAR-T cell products listed at China and abroad are all autologous pared with autologous CAR-T treatment, universal CAR-T exhibits significant advan‑tages, which could fulfill the treatment demand of more patients, but also displays high technical barriers.This paper reviews the universal CAR-T, clearly points out the two major challenges faced by the development of uni‑versal CAR-T, and then summarizes and analyzes the feasible solutions according to the mechanism causing the two major problems.This paper also summarizes domestic and foreign companies producing universal CAR-T and the latest clinical progress of their superior products, and then discusses the feasibility of the development strategy from another aspect, in order to provide ideas for developing a new generation of universal CAR-T cell therapy products.Key words CAR-T; allogeneic; immune rejection; gene editing; clinical research; progress近年来，免疫疗法已成为继手术治疗、放化疗和靶向药物治疗之后另一种新型的肿瘤治疗手段［1］。
- 26 -甲状腺激素水平的影响[J].淮海医药，2021，39（1）：54-56.[15]张永博.腔镜辅助颈部小切口甲状腺手术治疗甲状腺良性肿瘤的效果分析[J].河南外科学杂志，2020，26（6）：109-110.[16]林仁志，郑晨辉，钟吉俊，等.经口腔前庭入路腔镜甲状腺全切除术治疗cN0甲状腺乳头状癌临床研究[J].中国实用外科杂志，2020，40（10）：1194-1196，1201.[17] CAMENZULI C，WISMAYER P S，AGIUS J C. Transoralendoscopic thyroidectomy: a systematic review of the practice so far[J]. JSLS，2018，22（3）：26.（收稿日期：2023-06-22） （本文编辑：马娇）①蚌埠医学院第二附属医院 安徽 蚌埠 233000依达拉奉右莰醇治疗急性进展性大动脉粥样硬化型脑梗死的临床效果万雅迪①【摘要】 目的：探讨依达拉奉右莰醇治疗急性进展性大动脉粥样硬化（LAA）型脑梗死的临床效果。
方法：回顾性选取2021年11月—2023年4月蚌埠医学院第二附属医院收治的100例急性进展性LAA 型脑梗死患者的临床资料。
根据治疗方案的不同将其分为对照组（n =50）和观察组（n =50）。
对照组给予常规治疗，观察组在对照组基础上给予依达拉奉右莰醇治疗。
比较两组治疗前后氧化应激指标、炎症因子、神经功能和预后情况及不良反应。
结果：治疗后，观察组谷胱甘肽过氧化物酶（GSH-Px）水平高于对照组，活性氧簇（ROS）水平显著低于对照组，差异有统计学意义（P <0.05）。
治疗后，观察组肿瘤坏死因子-α（TNF-α）、基质金属蛋白酶9（MMP-9）水平均显著低于对照组，差异有统计学意义（P <0.05）。
治疗后3个月，两组美国国立卫生研究院卒中量表（NIHSS）评分、改良Rankin 量表（mRS）评分均降低，观察组NIHSS 评分、mRS 评分均显著低于对照组，差异有统计学意义（P <0.05）。
Energy recovery from secondary pulp/paper-mill sludge and sewage sludge with supercritical water treatment qLinghong Zhang a ,Chunbao (Charles)Xu b,*,Pascale Champagne a,caDepartment of Civil Engineering,Queen’s University,Kingston,ON,Canada K7L 3N6bDepartment of Chemical Engineering,Lakehead University,Thunder Bay,ON,Canada P7B 5E1cDepartment of Chemical Engineering,Queen’s University,Kingston,ON,Canada K7L 3N6a r t i c l e i n f o Article history:Received 17June 2009Received in revised form 12November 2009Accepted 30November 2009Available online 30December 2009Keywords:Secondary pulp/paper-mill sludge Sewage sludgeSupercritical water Synthetic gas Heavy oila b s t r a c tSecondary pulp/paper-mill sludge (SPP)and sewage sludges (primary,secondary,and digested sewage sludges)were treated in supercritical water at temperatures ranging between 400°C and 550°C over 20–120min for energy recovery.Low temperature and short reaction time favored the formation of heavy oil (HO)products,which were mainly composed of a variety of phenol and phenolic compounds,as well as some nitrogen-containing compounds,long-chain alkenes and alcohols,etc.,with high gross calorific values (>36MJ/kg).By contrast,the formation of synthetic gases,a mixture of hydrogen,carbon monoxide,carbon dioxide,methane,and other light hydrocarbons,were not significantly affected by reaction time but greatly enhanced with increasing temperature.The highest gas yield was obtained at 550°C,where 37.7wt.%of the SPP (on dry basis)was converted into gases,with hydrogen yields as high as 14.5mol H 2/kg SPP (on a dry basis).In comparison to sewage sludges,SPP exhibited a greater capabil-ity for the production of HO and gases owing to its higher contents of volatiles and alkali metals,indicat-ing a prospective utilization potential for SPP as a source of bio-energy.Ó2009Elsevier Ltd.All rights reserved.1.IntroductionSecondary pulp/paper-mill sludge (SPP)is the residue produced in the biological treatment process of wastewaters from the pulp/paper industry.It is a liquid suspension that contains approxi-mately 2wt.%solids,and is composed of cells,biodegradable and recalcitrant organic compounds (e.g.,carbohydrates and lignin),as wells as ashes.SPP can also be considered a waste biomass that can potentially be recovered into a series of solid (e.g.,charcoal),liquid (e.g.,bio-oils),and gaseous (e.g.,biogas and synthetic gas)bio-fuels using various biological and thermo-chemical processes,contributing little or no net greenhouse gas emissions to the envi-ronment because of its carbon–neutral lifecycle (Metcalf and Eddy,2003;Fytili and Zabaniotou,2008).Supercritical water (SCW)is a special water phase where the pressure and temperature of water are increased to or above their respective critical points (22.1MPa and 374°C).As a superior reac-tion medium,SCW is featured as having a high diffusivity,a low viscosity,and a high solvating ability for organic compounds (Gloyna et al.,1994).In addition,water is also an active reactantin steam reforming and water–gas shift reactions (shown below in Reactions 1and 2)in the supercritical region.Therefore,SCW is able to transform biomass feedstock into hydrogen (H 2)-rich gas-eous products with reduced tar and coke formation,and this pro-cess is commonly regarded as supercritical water gasification (SCWG)(Guo et al.,2007;Demirbas,2004;Osada et al.,2006;Ya-nik et al.,2007;Kruse et al.,2003).Steam reforming reaction:CH x O y þð1Ày ÞH 2O !CO þ1Ày þx 2H 2ð1ÞWater–gas shift reaction:CO þH 2O $CO 2þH 2ð2ÞFor most conventional thermo-chemical treatment processes,such as combustion,pyrolysis,and gasification,a pre-dried feed-stock is typically required (Furness et al.,2000).Considering the extremely high water content (>90%on wet mass basis)of sludges,the pre-drying operation would hence be energy and capital inten-sive.However,sludge can be treated directly with SCW,eliminat-ing the need for drying.In comparison to anaerobic digestion (biomethanation),a con-ventional biological method for the methane (CH 4)-rich biogas product generation from sludge,SCWG can be considered to be more advantageous in terms of providing a higher treatment0960-8524/$-see front matter Ó2009Elsevier Ltd.All rights reserved.doi:10.1016/j.biortech.2009.11.106qPart of this work has been presented and collected in the Proceedings of the 8th World Congress of Chemical Engineering,Montreal,Quebec,Canada,August 23–27,2009.*Corresponding author.Tel.:+18073438761;fax:+18073438928.E-mail address:cxu@lakeheadu.ca (C.Xu).Bioresource Technology 101(2010)2713–2721Contents lists available at ScienceDirectBioresource Technologyj o u r n a l ho m e p a g e :w w w.e l s e v i er.com/locate/biortechefficiency.SCWG requires much less reaction time(several sec-onds,minutes,to hours for SCWG vs.several days to weeks for anaerobic digestion)to accomplish(Bridgewater,2001)and is able to destroy lignin compounds,which are typically recalcitrant in anaerobic digestion.In addition,SCWG may be a more capitally effective process in comparison to biomethanation,as noted by Matsumura(2002).As such,the use of SCWG for the treatment of sludge for energy recovery was the focus of this research.A number of studies have recently been conducted on SCWG covering a wide range of biomass feedstocks,such as model com-pounds(e.g.,glucose,cellulose,lignin,and starch),fruit shells,saw-dust,rice straw,municipal solid waste,etc.However,there are very few investigations that have focused on the use of sludges as feedstocks.Xu et al.(1996)employed ground and homogenized sewage sludge,pumped as a slurry into a supercriticalflow-type reactor at a temperature of600°C and pressure of34.5MPa,using coconut shell activated carbon as the catalyst.They observed that with a low feedstock concentration(2.8wt.%)and a low weight hourly space velocity(WHSV)of0.5hÀ1,almost all of the sludge was converted into gaseous product,with a H2yield of13.5mol H2/kg sludge(dry basis).However,they did not attempt to use a higher solids concentration due to the experimental challenge associated with the pumping system.Xu and Antal(1998)over-came pumping issues by mixing the sludge in a cornstarch paste and utilizing a cement pump.By doing this,a higher sludge solids concentration(up to7.69wt.%)could be employed as a feedstock. In this experiment,the temperature and pressure were650°C and 28MPa,respectively,and a packed bed of coconut shell activated carbon was also used as the catalyst.At a low solid concentration (2.1wt.%sludge+5.1wt.%corn starch;flow rate: 2.0g/min; WHSV:1.48hÀ1),99%of the feed was converted to a gas product with a mole fraction of H2of42%.When a higher solids concentra-tion of the feedstock was used(7.69wt.%sludge+7.69wt.%corn starch),the fractions of carbon dioxide(CO2)and H2were found to decrease,while CH4increased,and carbon monoxide(CO)did not change significantly.However,reactor plugging occurred in both of these tests because of the high ash content of the sludge (25.1%,on a dry basis).Zhang et al.(2007)studied the effect of reaction temperature(400–600°C),pressure(24–30MPa),resi-dence time(3–15min),and catalyst(with and without sodium hydroxide)on the H2yield from a sewage sludge sample with a 95%water content,using a continuousflow reactor,in the presence of30wt.%hydrogen peroxide(H2O2)for partial oxidation.It was observed that higher temperature and pressure,longer residence time,and the presence of a sodium hydroxide(NaOH)catalyst, all contributed to the enhanced H2production in this SCWG and partial oxidation process.In addition,the authors evaluated the en-ergy efficiency for this process.In an ideal scenario assuming no external energy loss considering the energy input for pumping and heating,and taking into consideration the energy recovery from hot-pressured water,the estimated energy yield could reach 85%.As the overall reaction is endothermic,it was recommended that heat recovery from the hot-pressured water would be of sig-nificant importance for a better energy yield.Despite these limited studies on the SCWG of sewage sludge, there is very little documented information on the SCWG behavior of sludge from industrial sources(e.g.,from the pulp/paper indus-try),although Xu and Lancaster(2008)reported on conversion of secondary pulp/paper sludge to bio-oils by direct liquefaction in hot compressed or sub-and near-critical water at280–380°C.As such,the primary objective of this study was to investigate the po-tential of recovering energy from secondary pulp/paper sludge by SCWG.More specifically,the effects of reaction temperature,reac-tion time and sludge dry matter content(or water content)on product yields in the SCWG of SPP for H2production were investi-gated using a batch reactor.In addition,the various forms of prod-ucts recovered from this SCWG process were characterized with respects to chemical compositions.For comparison,three types of municipal sewage sludges:primary sewage sludge(PS),second-ary sewage sludge(SS)and digested sewage sludge(DS)were also examined under similar experimental conditions to those of SPP,to investigate the relationships between sludge composition and product yields.2.Methods2.1.MaterialsThe sludge feedstocks used in this work were the SPP,supplied by the AbitibiBowater Thunder Bay Corporation,and PS,SS,and DS supplied by the Thunder Bay Municipal Wastewater Treatment Plant.DS is the biosolids product resulting from the anaerobic digestion of the PS and SS,which is commonly disposed to landfill after further dewatering.The compositions,in terms of the water content,proximate and ultimate analyses,as well the mineral ele-mental compositions of each type of sludge are given in Tables1 and2.The solvents used in this work for product separation were distilled water and ACS reagent-grade ethyl acetate and acetone from Canadawide Scientific and used as received.The water content was determined by measuring the weight loss of each sludge sample before and after it was dried at105°C in an oven for at least12h.The volatile matter(VM)andfixed car-bon(FC)contents were tested by using a thermogravimetric analyzer(TGA)-i1000(Instrument Specialist Inc.):heating the pre-dried sample in a nitrogen atmosphere at10°C/min up to 900°C.The ash content was obtained by recording the weight of the remaining portion of the pre-dried sludge sample after it had been burned in a muffle furnace at575°C for8h.The elemental (e.g.,C,H,and N)composition was determined with a CEC(SCP) 240-XA elemental analyzer,and the composition of oxygen(O) was estimated by difference,assuming negligible amounts of sul-phur.In addition,the ash samples were analyzed for their mineral composition using a Varian Vista-PRO CCD Simultaneous ICP-OES (inductively coupled plasma-optical emission spectroscopy)with a Cetac ASX-510Autosampler.Specifically,each ash sample was digested in aqua regia(3:1HCl/HNO3)using microwave CEM Mars 5open vessels for3–4h,diluted with distilled deionized water and then injected into ICP using an inert spray chamber with a cross-flow nebulizer.The major operational conditions of the ICP-OES were:power:1.10kW;plasmaflow:15.0L/min;auxiliaryflow: 1.50L/min;nebulizerflow:0.75L/min;replicate time:15.000s; stabilization time:20s;and replicates:3.2.2.Sludge supercritical water gasificationThe SCWG tests were performed in a75mL Parr High-Pressure reactor,constructed of Hastelloy alloy,with maximum working pressure of41.37MPa at600°C.In each experimental run,approx-imately20g of raw/concentrated/diluted sludge was loaded into the reactor.The residual air in the reactor was completely removed with at least three cycles of vacuuming and nitrogen purging.The reactor was pressurized to2MPa using high-purity N2to prevent the boiling of water during heating,then heated at about10°C/ min to a specified temperature and maintained for a pre-deter-mined period of time(i.e.20,40,60,and120min),which repre-sented the reaction times investigated in this research.During each experimental run,the pressure inside the reactor was re-corded.Since the reaction pressure was a function of the reaction temperature inside the sealed batch reactor,it could not be ad-justed manually.The reaction pressures for the tests throughout the study were all noted to be above22.1MPa(the critical pressure2714L.Zhang et al./Bioresource Technology101(2010)2713–2721for water)at all reaction temperatures.After the desired reaction time elapsed,the reactor was cooled down rapidly to room tem-perature using a wetted cloth towel.2.3.Separation and analyses of the reaction productsGas products were collected in a gas cylinder and analyzed with an Agilent3000Micro-Gas Chromatograph(GC)equipped with dual columns(Molecular Sieve and PLOT-Q)and thermal conduc-tivity detectors(TCDs).The remaining solid/liquid products were recovered thoroughly from the reactor by washing with ethyl ace-tate solvent andfiltered under reduced pressure through a pre-weighed No.5filter paper.The solids retained on thefilter paper were oven dried at105°C for at least12h and weighed to obtain the weight of the solid residue(SR),while thefiltrate which was composed of an aqueous phase and an ethyl acetate soluble phase was separated in a separatory funnel.As the aqueousfiltrate was composed of water soluble products(WSPs)and the dissolved ethyl acetate,the total organic carbon(TOC)analysis results for the WSPs were not usable.As such,the TOC results for the WSPs were not reported in this paper.The ethyl acetate soluble phase was further transferred into a pre-weighed evaporationflask and evaporated at56°C at a reduced pressure of10kPa to remove the solvent.The remaining brown liquid was weighed and referred to as heavy oil(HO).The yields of total gas,HO and SR were defined as percentages (%)of the mass of each product in relation to the mass of dried matter in the sludge fed into the reactor prior to the reaction, and the yield of WSPs and pyrolytic water were lumped and calcu-lated by difference.Two to three duplicate runs were conducted for each experimental condition and the average yields were calcu-lated and reported.The error between replicate runs was main-tained to within5%of the yields for most of the gas and solid products while10%of the yields for most liquid products.Higher reproducibility for the liquid yields was challenging due to the dif-ficulty in liquid product separation and their low formation amounts in the SCWG process under investigation.The elemental compositions(C,H,and N)of selected HOs and SRs were determined using a CEC(SCP)240-XA elemental analyzer,and the composition of oxygen(O)in HOs was estimated by difference assuming negligible contents of sulphur and ash in the samples.In addition,the HO product samples from SCWG of PS,SS,DS,and SPP were dissolved in acetone and analyzed with a Shimadzu gas chromatograph/mass spectrometer(GC/MS)-QP2010S equipped with a SHRXI-5MS capillary column(30mÂ0.25mmÂ0.25l m). Approximately1l L of HO–acetone solution was injected into the instrument in a split mode with a split ratio of20.0.The column temperature was initially40°C,held for2min,and raised to 190°C at a heating rate of12°C/min.The column was then heated at a heating rate of8°C/min to290°C,followed by another isother-mal hold for30min at this temperature.The ion source and inter-face temperature were set at200°C and275°C,respectively.Data was acquired in a scan mode with a solvent cut time of2.5min, and the m/z ranged between40and500.The obtained chromato-graphic peaks were identified using the WILEY8library.3.Results and discussion3.1.Effects of temperatureThe product yields obtained at various reaction temperatures (400–550°C)are presented in Fig.1.The corresponding pressures inside the reactor were23.79MPa(400°C),30.34MPa(450°C), 37.00MPa(500°C),and45.51MPa(550°C),respectively,indicat-ing that water in all cases was in supercritical state.The gaseous products were mainly composed of H2,CO,CO2,CH4,and small quantities of light C2and C3compounds,such as ethylene(C2H4), ethane(C2H6),acetylene(C2H2),propane(C3H8),and propylene (C3H6).As expected,higher temperature facilitated the formation of gases,which is in good agreement with Demirbas(2004)and Zhang et al.(2007).In addition,an increase in the yield of SR and a decrease in the yield of HO were also observed as temperature was increased.Specifically,at the low temperature of400°C,the yield of gaseous products was16.4wt.%,while the yields of HO and SR were as high as28.8wt.%and29.1wt.%,respectively.As the temperature increased from400°C to500°C,the yield of gas products was improved considerably to30wt.%(almost double that observed at400°C)at the expenses of HO(17wt.%yield) and SR(26wt.%yield).As the temperature was increased further,Table1Properties of PS,SS,DS,and SPP.Type of sludge Water content(wt.%)a pH Proximate analysis(wt.%)(d.b.b)Ultimate analysis(wt.%)(d.b.b)HHV d(MJ/kg)VM FC Ash C H N O cPS97.2567.810.721.540.3 5.17 3.2829.815.7 SS95.5860.112.227.737.9 4.77 5.8523.815.4 DS97.2848.99.3041.831.6 3.66 3.8819.112.5 SPP98.0960.615.024.441.2 4.51 4.1825.715.8a Water content=100%Àdry matter content.b On a dry basis.c By difference(O%=100%Àash%ÀC%ÀH%ÀN%)assuming negligible sulphur content.d Higher heating value(HHV)calculated by the Dulong Formula,i.e.,HHV(MJ/kg)=0.3383C+1.422(H–O/8).Table2Mineral elemental compositions of PS,SS,DS,and SPP a.Type of sludge Major mineral elements in the sample(wt.%)(d.b.b)Na K Mg Ca Mn Fe Zn Al Si P SPS0.2410.3400.555 1.9110.0290 3.3170.0338 1.570.0903 1.260.252 SS0.3620.7200.562 2.9710.1447.3310.0509 5.810.194 4.610.573 DS0.4700.7580.918 4.1090.136 6.3680.0820 4.800.128 4.280.791 SPP7.0410.5340.808 1.4230.03410.4760.0606 1.650.07640.867 2.33a Determined by ICP-AES.b On a dry basis.L.Zhang et al./Bioresource Technology101(2010)2713–27212715from 500°C to 550°C,the gas yield was further increased at the ex-pense of HO,suggesting cracking of the heavy oil product into gases.From a thermodynamic perspective,a high temperature fa-vors gas formation,since the overall biomass SCWG process is endothermic (Guo et al.,2007).As the temperature was increased from 500°C to 550°C,how-ever,a surprisingly large increase in the yield of SR,from 26wt.%at 500°C to 39wt.%at 550°C,was observed.Similar results,where the formation of SR (or char)increased with temperature,were ob-served in previous work by Xu and Lancaster (2008),when liquefy-ing SPP in hot-compressed water (280–380°C).The increase in char formation at higher temperatures could be attributed to the condensation of the lighter fragments (intermediates)derived from cellulose and lignin compounds in the system (Osada et al.,2006;Yanik et al.,2007).For example,phenolic compounds and formaldehydes,both of which are hydrolysis products from lignin,may polymerize into high molecular-weight condensed products or solid chars via condensation/cross-linking reactions.These phe-nol and phenolic compounds may not only be decomposed from lignin compounds,but can also be converted from glucose/fruc-tose,the products of cellulose hydrolysis (Kruse and Gawlik,2003).Hence,it is likely that the increase in gases and solids re-sulted from the cracking or the condensation of the phenolic com-pounds in the HO.Additionally,it has been widely accepted that as temperature increases,the dehydration of low-molecular-weight carbohydrates and some soluble organic acids,present in the WSPs as a product of lignocellulosic or cellulose feedstock hydrolysis,would occur at a high temperature to form oily intermediates which would further be condensed into char (Osada et al.,2006).As shown in Fig.1a,the net increase in SR yield ($14wt.%)was al-most balanced by a corresponding decrease in WSPs +H 2O yield as temperature increased from 500°C to 550°C,implying the possi-bility of WSPs dehydration to form oily intermediates,followedby the condensation of these intermediates into char at 500–550°C.The production of H 2and other combustible gases were of pri-mary interest in this study.The yields of each main gaseous com-pound (e.g.,H 2,CO,CO 2,and CH 4)in mole per kg of sludge (on a dry basis)are presented in Fig 1b.As was expected,the yield of all indi-vidual gas components (except CO)increased continuously with temperature.In particular for H 2and CH 4formation,significant in-creases were observed between 500°C and 550°C.Overall,increasing the temperature from 400°C to 550°C led to an almost 10-fold increase in the yields of H 2and CH 4,the H 2yield reaching as high as 14.5mol/kg dried sludge at 550°C,which was compara-ble to the results reported by Xu et al.(1996)who obtained a H 2yield of 13.5mol H 2/kg sewage sludge (dry basis)at 600°C,34.5MPa and WHSV of 0.5h À1with a supercritical flow-type reac-tor using coconut shell activated carbon as the catalyst.3.2.Effects of reaction timeThe product yields and the formation of the main gaseous prod-ucts (H 2,CO 2,CO and CH 4)from the SCWG of raw SPP (dry matter content:2wt.%)at 500°C over various reaction times (20–120min)are shown in pared with temperature,reaction time had a lower influence on SR yields and total gas yields (Fig.2a),as well as the yield of individual gaseous species such as H 2,CO,CO 2and CH 4(Fig.2b).These results were generally con-sistent with previous work by Xu and Lancaster (2008)treating a similar SPP feedstock in hot-compressed water.The results (Fig.2a)indicated that reaction time significantly affected the dis-tribution of the liquid products between HO and WSPs,where the lumped WSPs +H 2O yield increased at the expense of the HO yield as reaction time increased from 20min to 120min at 500°C.Wil-liams and Onwudili (2005)investigated the effect of reactiontimeFig.1.Effects of temperature on product distribution in different phases (a),and yields of main gaseous products (H 2,CO 2,CO and CH 4)(b)from SCWG of raw SPP for 60min.Fig.2.Effects of reaction time on product distribution in different phases (a),and yields of main gaseous products (H 2,CO 2,CO and CH 4)(b)from SCWG of raw SPP at 500°C.2716L.Zhang et al./Bioresource Technology 101(2010)2713–2721on product distribution using glucose as the model compound for biomass,and similar results were obtained where a significant de-crease in HO yield corresponded to a higher WSPs yield at a longer reaction time.The above result implies the conversion of HO to WSPs with longer residence time,which is probably via hydration reactions.However,the mechanism governing the conversion of HO into WSPs is not yet clear.Future work is thus needed.3.3.Effects of dry matter content (water content)in the sludge feedstockIn this study,the raw SPP with an original solid concentration of 2.0wt.%was thickened by vaporization of water to produce con-densed feedstock with dry matter contents of 6.0wt.%and 8.8wt.%,respectively,to investigate the effects of dry matter con-tent on product yields.The results are illustrated in Fig.3.It can be noted from Fig.3a that the change in feedstock dry matter content did not result in a significant change in the SR yield.However,the dry matter content of the feedstock did influence the yields of gas-eous and liquid products.Generally,an increase in the dry matter content of the feedstock from 2wt.%to 8.8wt.%was accompanied by decreased yields of HO and total gas (Fig.3a),but an increased WSPs yield.An increase in the dry matter content of the feedstock also led to significant reductions in the formation of H 2and CO 2,while the effect of feedstock dry matter content on the formation of CO and CH 4gases was minimal (Fig.3b).Similar results were re-ported in other work by Kruse et al.(2003)and Onwudili and Wil-liams (2007),where a lower water density (or higher dry matter content)decreased H 2production.Kruse (2008)has summarized the following two equations for H 2and CH 4formation from glu-cose,respectively:Hydrogen formation :C 6H 12O 6þ6H 2O !6CO 2þ12H 2ð3ÞMethane formation :C 6H 12O 6!3CO 2þ3CH 4ð4ÞIt can be seen that 6mol of water is required to convert 1mol of C 6H 12O 6into H 2-rich gas,whereas no water is needed for CH 4for-mation.Therefore,H 2formation was likely suppressed as the water content decreased.3.4.Effects of different sources of sludgeFour types of raw sludge as received,i.e.,PS,SS,and DS from a municipal source,and the SPP from an industrial source,were investigated for their potential for H 2production via SCWG.The products yields at 500°C for 60min are displayed in Fig.4.Clearly,SPP appeared to be the best candidate among these four feedstocks since it exhibited the highest yields of valuable products such as H 2,CH 4,and oil.Conversely,DS showed the least potential due to its low yields of gas and oil,but a high yield of SR.To eliminate any possible influence caused by the different lev-els of water content (dry matter content),the three municipal sew-age sludges:PS,SS,and DS,were diluted with distilled water to obtain a similar dry matter content ($2wt.%)as that of SPP to eval-uate their potentials for gas production in SCW.SCWG of these sludge feedstocks was also performed at 500°C for 60min,and the yields of gas,HO,and solid SR products,as well as the yields of the major gaseous components are illustrated in Fig.5.Obviously,product distribution and gas formation are strongly dependent on the type of sludge (or the properties of the feed-stock).Similar to that observed from Fig.4,SCWG of SPP produced the highest yields of total gas,HO as well as the yields of H 2,CH 4and CO 2.With respect to the production of H 2and CH 4and HO,the four types of sludges tested in this work showed the following priority sequence:SPP >PS >SS >DS.The difference in the perfor-mance of these feedstocks may be associated with their distinct compositional characteristics,such as the contents of volatile mat-ter and ash as well as the ash compositions (alkali metalcontent).Fig.3.Effects of dry matter content on product distribution in different phases (a)and yields of main gaseous products (H 2,CO 2,CO and CH 4)(b)from SCWG of SPP of various water contents at 500°C for 60min.Fig.4.Effects of various types of sludges (raw)on product distribution in different phases (a)and yields of main gaseous products (H 2,CO 2,CO and CH 4)(b)from the SCW treatment at 500°C for 60min.L.Zhang et al./Bioresource Technology 101(2010)2713–27212717Specifically,the low yields of total gas and individual gas species of H 2,CH 4and CO 2from DS was likely due to its low content of vol-atile matter (Table 1).Moreover,an alkaline environment was pro-ven to decrease the degradation temperature of cellulose,a major component in biomass material,and to reduce the yields of tars/chars by suppressing the formation of tar-precursor compounds such as furfurals and 5-hydroxylmethylfurfural (5-HMF)(Onwudili and Williams,2009;Matsumura et al.,2005).In addition,the pres-ence of alkali is also effective for improving biomass gasification and H 2formation due to its catalytic effects on the water–gas shift reaction (Reaction 2)through the mechanism involving the forma-tion of metal formate and CO (Zhang et al.,2007;Kruse et al.,2003;Onwudili and Williams,2009).Furthermore,Onwudili and Williams,2009)proposed that the removal of CO 2as sodium car-bonate or bicarbonate in a NaOH surrounding also contributed to shift the water–gas reaction towards the formation of hydrogen gas.As shown in Table 2,the SPP feedstock contained high concen-trations of alkali metals,e.g.,7.04wt.%Na in relation to <0.5%Na for the other feedstocks (PS,SS,and DS).In addition,it also exhib-ited the highest pH among these sludges (Table 1).These two fac-tors might account for the greater yields of total gas and H 2from SPP.To validate this explanation,several drops of concentrated NaOH solution were added to PS to increase its pH from 5to 9(la-beled as PS 00in Fig.5).As predicted,greatly enhanced yields of gas-eous species (H 2and CH 4and CO 2),with the exception of CO were observed (Fig.5a).As shown in Fig.5b,it can be seen that the H 2yield from PS 00was almost six times that of PS,accompanied by a great increase in CO 2yield and a drastic decrease in CO yield,sug-gesting a catalytic effect of NaOH on the water–gas shift reactions.Although different from what was obtained by Onwudili and Wil-liams (2009)where a significant amount of CO 2was converted into carbonates or bicarbonates in the aqueous phase,the carbonates or bicarbonates-combined CO 2would not be significant in the study due to the acidity of the aqueous phase (pH $6).Moreover,the dissolved CO 2in the aqueous phase was less significant either(accounting for only 3–4%of the amount of CO 2in the gaseous phase,based on Henry’s Law calculation).Accordingly,the CO 2formed in this study was believed mainly present in the gaseous phase.In addition,as shown in Table 2,although SPP had a higher Na content than SS,SS contained higher levels of Ca,Fe,Al,and P.Ca and Fe have been noted by other researchers to be effective in enhancing hydrogen yield from coal or biomass material via SCWG (Wang and Takarada,2001;Yanik et al.,2008),therefore they might have promoted hydrogen production from SS to some extent as well.However as shown in Fig.5,despite that SPP and SS exhib-ited similar VM contents (Table 1),the hydrogen yield from SS was much lower than that from SPP.One possible reason was the pH:the pH of SS was lower than that in SPP (8in SS vs.9in SPP).It is also possible that the catalytic effects of Ca and Fe were not as obvious as that of Na,or that the presence of P or other compounds exerted negative influence and thus decreasing hydrogen yield.Fu-ture work is needed to clarify the contribution of each compound in this complex reaction system.3.5.Characterization of the liquid/solid productsCarbon balance has been commonly employed to evaluate the material balance for various biomass conversion processes.It is,however,very difficult to test the level of TOC in the aqueous phase due to the presence of the dissolved ethyl acetate.In addition,for most tests the mass of solid feed was very small (i.e.approximately 0.4g),hence,the quantities of recovered products were not large enough for CHN elemental analysis.Consequently,carbon balance analysis was not performed in the present study.However,the fractions of recovered carbons from the gaseous products (e.g.,CO,CO 2,CH 4,C 2H 4,C 2H 6,C 3H 8,C 3H 6,etc.)in all the tests have been calculated and were found to fall into the range of 12.6–33.6wt.%.In addition,the CHN elemental analysis has been performed on the HO and SR products from the condensed SPP (with the dry matter content of 8.8wt.%)and the original SS (with the dry matter con-tent of 4.5wt.%).For both tests,approximately 30wt.%of the total carbon from the feedstock went into the HOs (32.1wt.%for the condensed SPP and 30.0for the original SS),while only less than 8wt.%of the carbon retained in SRs (7.33wt.%for the condensed SPP and 4.29for the original SS),indicating high biomass conver-sion efficiencies.As the byproducts of the SCWG process,the elemental and chemical compositions of the HOs are of particular interest in this work.The elemental (C,H,and N)analysis results for the HOs from SCWG of the condensed SPP and the original SS are presented in Table 3.From this table it can be seen that both HOs exhibited very high carbon contents (i.e.$80%)and very low oxygen content (i.e.$9%)compared with the respective feedstock (Table 1).This trans-lates to much higher gross calorific values of the oils (>36MJ/kg),more than twice those for the original dried sludge powders.The increased energy density from sludge to HO would provide a great-er ease of utilization,storage and transportation of the liquid fuels.In addition,the calculated empirical formula shows that the organ-ic compounds within the HO have a high degree of unsaturation,indicating the presence of aromatics of benzene,phenol,or their derivatives.Table 4presents the GC/MS analysis results of the HO products obtained from SCWG of the four types of raw sludge at 500°C for one hour.The chemical compounds were identified using the WI-LEY8library based on the GC/MS chromatographs.The relative area%for each compound identified (defined by percentage of the compound’s chromatographic area out of the total area)is shown in the table.To better understand the contents,a typical chromatogram of the HO product is also displayed (Fig.6).It can be seen that the compositional differences were relativelysmallFig.5.Effects of various types of sludges (whose dry matter contents were adjusted to approximately 2wt.%)on product distribution in different phases (a)and yields of main gaseous products (H 2,CO 2,CO and CH 4)(b)from SCWG at 500°C for 60min.2718L.Zhang et al./Bioresource Technology 101(2010)2713–2721。
壁虎提取物抗小鼠H22肝癌及免疫调节罗文军王建刚★（河南科技大学医学院药理教研室，洛阳，471003）摘要目的研究壁虎提取物(gekko extract,GE)抗肿瘤活性及其免疫学机制。
方法采用小鼠H22肝癌移植模型，观察GE对肿瘤生长的抑制,免疫器官的影响,外周血白细胞计数和ELISA检测试剂盒测血清中IL-12，IL-18含量。
结果GE高中低剂量组均对H22荷瘤小鼠均具有明显的抑制作用抑制率分别为55.25%,47.89%,15.16%，与模型组、阳性组相比差异显著。
高中低剂量的荷瘤小鼠脾脏胸腺质量及其指数外周血白细胞计数，血清中IL-12，IL-18含量同空白及阳性组相比差异均非常显著。
结论GE抑制肿瘤的生长，增强荷瘤小鼠免疫功能，其机制可能与其促进白细胞增殖及IL-12，IL-18分泌有关。
关键词壁虎提取物; 抗瘤; 免疫调节Immunoregulation and antitumor of gekko extract in H22 tumor mice ABSTRACT Objective To investigate antitumor activity and it’s immunological mechanisms of GekkoExtract(GE).Methods H22 tumor-bearing model KM mice were applied by intraperitoneal injection toevaluated in vivo antitumor activity and immunoregulatory effect of GE. The influence of different doseGE(0.4,0.8,1.6g/kg) on immune organs and antitumor effects in tumor-bearing mice were observed.The levels of IL-12 and IL-18 in serum from mice were detected by means of ELISA Kits. Results GE possessedmarkedly inhibitory activity on the growth of H22 tumor in mice,the dosage of gekko extract were1.60，0.80 or 0.40 mg·Kg-1, theirs inhibitory rates were 55.25％、47.89％and l5.16％respectively with H22 liver cancer.To compare with model group、Positive group，theirs' difference was obvious,and had distinct effect on weight of thymus and spleen was observed,peripheral blood white blood cells were proliferated,the contents of serum IL-12/ IL-18 were induced significantly. Conclusion GE not only shows obvious antitumor effect,but also protects thymus and spleen, GE s Immunoregulation can Induce WBC,IL-12 and IL-18 productionKEYWORDS:GE; antitumor; immunoregulation壁虎作为我国传统中药，具有补肺肾、益精血、止咳定喘、镇痉祛风和发散消肿的功效。
纳米雄黄抗B细胞非霍奇金淋巴瘤Raji细胞的体外作用研究姜爽;王晓波;张治然;孙岚;李劲草;张英鸽【摘要】Aim To observe the effects of realgar nano-particles on B cell non-Hodgkin's lymphoma Raji cells in vitro. Methods Realgar nanoparticles and crude realgar particles were characterized with a laser particle size analyzer, a transmission electron microscopy (TEM)and an atomic force microscopy(AFM). The morphological changes of proliferation of Raji cells brought about by the use of realgar naoparticles and crude realgar particles were observed with a light mi-croscope. The membrane changes of Raji cells treated with realgar naoparticles and crude realgar particles were observed with AFM. The ultrastructures of Raji cells were observed with TEM. The inhibitory effects of Raji cells treated with realgar naoparticles and crude realgar particles were measured with MTT. The nuclear apoptosis morphologies of Raji cells were observed with fluorescence microscopy. The apoptosis rates and the cell cycle distributions of Raji cells treated with real-gars were measured with flow cytometry. Results The size of realgar nanoparticles and crude realgar particles was (79 ± 8)nm and (1. 89 ± 0. 2)μm,respectively. Light microscopy showed that realgar nanoparticles could inhibit the aggregation growth of Raji cells. AFM showed that Raji cells treated with realgar nanoparticle became shrank, had smaller volume and lost the growth state of stretching out. Raji cells treated with crude realgars did not change significantly. TEM showed Raji cells treated with realgarnanoparticle had damaged subcellular organelles and mitochondria with increased vacuoles. The Raji cells treated with crude realgar did not change significantly. MTT assay showed that when treated with the final concentration of 50 mg ·L - 1 of realgar nanoparticle for 24 h,the cell survival rate of Raji cells was (40 ± 2)% . When treated with the same concentration of crude realgar,its survival rate was (65 ± 3)% . When treated with 50 mg·L - 1 of realgar nanoparticle for 48 h,its survival rate was only 10 % ,and when treated with crude realgar ,its survival rate was (42 ± 2 )% . Fluorescence micro-scope indicated that the Raji cells treated with realgar nanoparticle had obvious nuclear apoptosis,which was not obvious in crude realgar group. Flow cytometry showed that the total apoptosis rate of Raji cells in-duced by realgar nanoparticles and by crude realgar was 11. 14%,15. 9%,respectively. Compared with those treated with crude realgar,the Raji cells treated with realgar nanoparticles presented a significantly higher ratio cell distribution in G1 phase and an obvious decreased ratio in S phase. Conclusion Compared with crude realgar particles,the same dose of realgar nanoparticles can significantly inhibit the proliferation of Raji cells,destroy their sub-cellular structure,and induce the cell apoptosis of Raji cells.%目的考察纳米雄黄对B细胞非霍奇金淋巴瘤Raji细胞的体外作用.方法利用激光粒度仪、TEM和AFM对纳米雄黄和水飞雄黄进行表征;利用光镜、AFM和TEM依次观察纳米雄黄和水飞雄黄作用下Raji细胞的增殖形态变化、单细胞表面细胞膜变化以及细胞内超微结构的变化;MTT法检测纳米雄黄和水飞雄黄作用下的细胞存活率;利用荧光显微镜及流式细胞术观察纳米雄黄和水飞雄黄引起Raji细胞的凋亡和细胞周期分布情况.结果纳米雄黄的粒径为(79±8)nm,水飞雄黄的粒径为(1.89±0.2)μm.光镜下,可明显观察到纳米雄黄可抑制Raji细胞的聚集生长状态,AFM下可观察到纳米雄黄作用下的Raji细胞皱缩,体积变小,膜表面的黏附物质不再向四周伸展,而水飞雄黄作用下的Raji细胞变化不明显.TEM下可观察到纳米雄黄作用下的Raji细胞胞内亚细胞器受到破坏,线粒体空泡明显增多,水飞雄黄组变化不明显.MTT结果显示,50 mg·L-1纳米雄黄作用Raji 细胞24 h时,细胞的存活率为(40±2)%,而相同剂量的水飞雄黄作用组为(65±3)%;50 mg·L-1纳米雄黄作用Raji细胞48 h时,Raji细胞的存活率仅为10%,而相同剂量的水飞雄黄组,Raji细胞的存活率为(42±2)%.荧光显微镜下可观察到纳米雄黄作用下的Raji细胞核凋亡明显,水飞雄黄组作用不明显.流式细胞术结果显示,水飞雄黄作用下的Raji细胞总凋亡率为11.14%,而纳米雄黄处理组的Raji细胞总凋亡率为15.9%.与水飞雄黄相比,纳米雄黄作用下Raji细胞在G 1期的分布比例明显升高,S期分布比率下降.结论与水飞雄黄相比,相同剂量的纳米雄黄在相同作用时间下,可明显抑制B细胞淋巴瘤Raji细胞的增殖,破坏其亚细胞结构,进而引起其凋亡.【期刊名称】《中国药理学通报》【年(卷),期】2017(033)012【总页数】9页(P1721-1729)【关键词】雄黄;纳米雄黄;水飞雄黄;B细胞淋巴瘤;非霍奇金淋巴瘤;细胞凋亡【作者】姜爽;王晓波;张治然;孙岚;李劲草;张英鸽【作者单位】解放军第 210 医院药剂科,辽宁大连 116021;解放军第 210 医院药剂科,辽宁大连 116021;解放军第 210 医院药剂科,辽宁大连 116021;军事医学科学院毒物药物研究所纳米药理毒理室,北京 100850;军事医学科学院毒物药物研究所纳米药理毒理室,北京 100850;军事医学科学院毒物药物研究所纳米药理毒理室,北京 100850【正文语种】中文【中图分类】R282.760.5;R329.24;R329.25;R733.402.2;R944.9在我国，非霍奇金淋巴瘤(non-Hodgkin’s lymphoma, NHL)占全部淋巴瘤病例的90%左右，其发病率和死亡率居恶性肿瘤第 5 位。
櫄櫄櫄櫄櫄櫄櫄櫄櫄櫄櫄櫄櫄櫄櫄櫄櫄櫄櫄櫄櫄櫄櫄櫄櫄櫄櫄櫄櫄櫄櫄櫄櫄櫄櫄櫄櫄櫄櫄櫄櫄櫄櫄櫄櫄櫄［２８］任 慧，张 常，范晓军．昆虫几丁质酶的分类、功能分析、重组表达及酶学性质的研究进展［Ｊ］．农业生物技术学报，２０１４，２２（４）：５０２－５０７．［２９］陶如玉，李 妍，马慧玲，等．家蝇ＡＭＰ１７基因的克隆及其分子特性和表达模式研究［Ｊ］．中国病原生物学杂志，２０１７，１２（１１）：１０７９－１０８３，１０９８．［３０］ＳｈｉＺＫ，ＷａｎｇＳＧ，ＺｈａｎｇＴ，ｅｔａｌ．ＴｈｒｅｅｎｏｖｅｌｔｒｅｈａｌａｓｅｇｅｎｅｓｆｒｏｍＨａｒｍｏｎｉａａｘｙｒｉｄｉｓ（Ｃｏｌｅｏｐｔｅｒａ：Ｃｏｃｃｉｎｅｌｌｉｄａｅ）：ｃｌｏｎｉｎｇａｎｄｒｅｇｕｌａｔｉｏｎｉｎｒｅｓｐｏｎｓｅｔｏｒａｐｉｄｃｏｌｄａｎｄｒｅ－ｗａｒｍｉｎｇ［Ｊ］．３Ｂｉｏｔｅｃｈ，２０１９，９（９）：３２１．［３１］ＴａｔｕｎＮ，ＶａｊａｒａｓａｔｈｉｒａＢ，ＴｕｎｇｊｉｔｗｉｔａｙａｋｕｌＪ，ｅｔａｌ．Ｉｎｈｉｂｉｔｏｒｙｅｆｆｅｃｔｓｏｆｐｌａｎｔｌａｔｅｘｏｎｔｒｅｈａｌａｓｅａｃｔｉｖｉｔｙａｎｄｔｒｅｈａｌａｓｅｇｅｎｅｅｘｐｒｅｓｓｉｏｎｉｎｔｈｅｒｅｄｆｌｏｕｒｂｅｅｔｌｅ，Ｔｒｉｂｏｌｉｕｍｃａｓｔａｎｅｕｍ（Ｃｏｌｅｏｐｔｅｒａ：Ｔｅｎｅｂｒｉｏｎｉｄａｅ）［Ｊ］．ＥｕｒｏｐｅａｎＪｏｕｒｎａｌｏｆＥｎｔｏｍｏｌｏｇｙ，２０１４，１１１（１）：１１－１８．［３２］ＲｉｚｚｏＡＭ，ＮｅｇｒｏｎｉＭ，ＡｌｔｉｅｒｏＴ，ｅｔａｌ．ＡｎｔｉｏｘｉｄａｎｔｄｅｆｅｎｃｅｓｉｎｈｙｄｒａｔｅｄａｎｄｄｅｓｉｃｃａｔｅｄｓｔａｔｅｓｏｆｔｈｅｔａｒｄｉｇｒａｄｅＰａｒａｍａｃｒｏｂｉｏｔｕｓｒｉｃｈｔｅｒｓｉ［Ｊ］．ＣｏｍｐａｒａｔｉｖｅＢｉｏｃｈｅｍｉｓｔｒｙａｎｄＰｈｙｓｉｏｌｏｇｙ（ＰａｒｔＢ：ＢｉｏｃｈｅｍｉｓｔｒｙａｎｄＭｏｌｅｃｕｌａｒＢｉｏｌｏｇｙ），２０１０，１５６（２）：１１５－１２１．［３３］ＬａｐａｒｉｅＭ，ＬａｒｖｏｒＶ，ＦｒｅｎｏｔＹ，ｅｔａｌ．Ｓｔａｒｖａｔｉｏｎｒｅｓｉｓｔａｎｃｅａｎｄｅｆｆｅｃｔｓｏｆｄｉｅｔｏｎｅｎｅｒｇｙｒｅｓｅｒｖｅｓｉｎａｐｒｅｄａｔｏｒｙｇｒｏｕｎｄｂｅｅｔｌｅ（ＭｅｒｉｚｏｄｕｓＳｏｌｅｄａｄｉｎｕｓ；Ｃａｒａｂｉｄａｅ）ｉｎｖａｄｉｎｇｔｈｅＫｅｒｇｕｅｌｅｎＩｓｌａｎｄｓ［Ｊ］．ＣｏｍｐａｒａｔｉｖｅＢｉｏｃｈｅｍｉｓｔｒｙａｎｄＰｈｙｓｉｏｌｏｇｙ（ＰａｒｔＡ：Ｍｏｌｅｃｕｌａｒ＆ＩｎｔｅｇｒａｔｉｖｅＰｈｙｓｉｏｌｏｇｙ），２０１２，１６１（２）：１２２－１２９．黄友明，曾晓春．茉莉酸甲酯对高温下抽穗扬花期水稻叶片生理特性的影响［Ｊ］．江苏农业科学，２０２３，５１（２）：６６－７１．ｄｏｉ：１０．１５８８９／ｊ．ｉｓｓｎ．１００２－１３０２．２０２３．０２．００９茉莉酸甲酯对高温下抽穗扬花期水稻叶片生理特性的影响黄友明，曾晓春（宜春学院生命科学与资源环境学院，江西宜春３３６０００） 摘要：研究茉莉酸甲酯（ＭｅＪＡ）对抽穗扬花期水稻的抗高温效应，为水稻农业生产和杂交制种中抽穗扬花期抵抗和避开高温危害等问题提供理论依据。
Rank ISSN Abbreviated JournalTitleFull Title Category2940007-9235CA-CANCER J CLIN CA-A CANCER JOURNAL FOR CLINICIAN医学10200028-4793NEW ENGL J MED NEW ENGLAND JOURNAL OF MEDICINE医学3410009-2665CHEM REV CHEMICAL REVIEWS化学17260140-6736LANCET LANCET医学63341474-1776NAT REV DRUG DISCOV NATURE REVIEWS DRUG DISCOVERY医学51741087-0156NAT BIOTECHNOL NATURE BIOTECHNOLOGY工程技术10020028-0836NATURE NATURE综合性期刊32570732-0582ANNU REV IMMUNOL ANNUAL REVIEW OF IMMUNOLOGY医学62471471-0072NAT REV MOL CELL BIO NATURE REVIEWS MOLECULAR CELL B生物63331474-175X NAT REV CANCER NATURE REVIEWS CANCER医学62461471-0056NAT REV GENET NATURE REVIEWS GENETICS生物63621476-1122NAT MATER NATURE MATERIALS工程技术16260098-7484JAMA-J AM MED ASSOC JAMA-JOURNAL OF THE AMERICAN ME医学63321474-1733NAT REV IMMUNOL NATURE REVIEWS IMMUNOLOGY医学75191748-3387NAT NANOTECHNOL Nature Nanotechnology工程技术12390036-8075SCIENCE SCIENCE综合性期刊27400306-0012CHEM SOC REV CHEMICAL SOCIETY REVIEWS化学14560066-4146ANNU REV ASTRON ASTR ANNUAL REVIEW OF ASTRONOMY AND地学天文75331749-4885NAT PHOTONICS Nature Photonics物理15680092-8674CELL CELL生物67681548-7091NAT METHODS NATURE METHODS生物62451471-003X NAT REV NEUROSCI Nature Reviews Neuroscience NEUROSCIENCES 14570066-4154ANNU REV BIOCHEM ANNUAL REVIEW OF BIOCHEMISTRY生物11940034-6861REV MOD PHYS REVIEWS OF MODERN PHYSICS物理48751061-4036NAT GENET NATURE GENETICS生物15060079-6425PROG MATER SCI PROGRESS IN MATERIALS SCIENCE工程技术51051078-8956NAT MED NATURE MEDICINE医学11140031-9333PHYSIOL REV PHYSIOLOGICAL REVIEWS医学15100079-6700PROG POLYM SCI PROGRESS IN POLYMER SCIENCE化学76261755-4330NAT CHEM Nature Chemistry化学62371470-2045LANCET ONCOL LANCET ONCOLOGY医学74001740-1526NAT REV MICROBIOL NATURE REVIEWS MICROBIOLOGY生物66151535-6108CANCER CELL CANCER CELL医学67091543-5008ANNU REV PLANT BIOL ANNUAL REVIEW OF PLANT BIOLOGY生物63191473-3099LANCET INFECT DIS LANCET INFECTIOUS DISEASES医学150001-4842ACCOUNTS CHEM RES ACCOUNTS OF CHEMICAL RESEARCH化学80331934-5909CELL STEM CELL Cell Stem Cell生物57941364-6613TRENDS COGN SCI TRENDS IN COGNITIVE SCIENCES医学63351474-4422LANCET NEUROL LANCET NEUROLOGY医学14660066-4308ANNU REV PSYCHOL ANNUAL REVIEW OF PSYCHOLOGY医学50621074-7613IMMUNITY IMMUNITY医学19000163-769X ENDOCR REV ENDOCRINE REVIEWS医学580001-8732ADV PHYS ADVANCES IN PHYSICS物理17250140-525X BEHAV BRAIN SCI BEHAVIORAL AND BRAIN SCIENCES医学76141754-5692ENERG ENVIRON SCI Energy & Environmental Science化学74791745-2473NAT PHYS Nature Physics物理29820370-1573PHYS REP PHYSICS REPORTS-REVIEW SECTION 物理65501529-2908NAT IMMUNOL NATURE IMMUNOLOGY医学61991465-7392NAT CELL BIOL NATURE CELL BIOLOGY生物84372159-8274CANCER DISCOV Cancer discovery ONCOLOGY 18180147-006X ANNU REV NEUROSCI ANNUAL REVIEW OF NEUROSCIENCE医学60511433-8351LIVING REV RELATIV Living Reviews in Relativity物理28850360-1285PROG ENERG COMBUST PROGRESS IN ENERGY AND COMBUST工程技术医学68241553-4006ANNU REV PATHOL-MECH Annual Review of Pathology-Mechanisms o14640066-4278ANNU REV PHYSIOL ANNUAL REVIEW OF PHYSIOLOGY医学32580732-183X J CLIN ONCOL JOURNAL OF CLINICAL ONCOLOGY医学29200362-1642ANNU REV PHARMACOL ANNUAL REVIEW OF PHARMACOLOGY 医学1520003-4819ANN INTERN MED ANNALS OF INTERNAL MEDICINE医学地学天文39930935-4956ASTRON ASTROPHYS REV ASTRONOMY AND ASTROPHYSICS REV71031614-4961LIVING REV SOL PHYS LIVING REVIEWS IN SOLAR PHYSICS ASTRONOMY & AS 67881550-4131CELL METAB Cell Metabolism生物39970935-9648ADV MATER ADVANCED MATERIALS工程技术76391756-1833BMJ-BRIT MED J BMJ-British Medical Journal MEDICINE, GENER 36650893-8512CLIN MICROBIOL REV CLINICAL MICROBIOLOGY REVIEWS医学1830003-9926ARCH INTERN MED ARCHIVES OF INTERNAL MEDICINE医学10940031-6997PHARMACOL REV PHARMACOLOGICAL REVIEWS医学680002-5100ALDRICHIM ACTA ALDRICHIMICA ACTA化学11890034-4885REP PROG PHYS REPORTS ON PROGRESS IN PHYSICS物理生物26600301-5556ADV ANAT EMBRYOL CEL ADVANCES IN ANATOMY EMBRYOLOGY化学14630066-426X ANNU REV PHYS CHEM ANNUAL REVIEW OF PHYSICAL CHEMIS5210016-5085GASTROENTEROLOGY GASTROENTEROLOGY医学51251081-0706ANNU REV CELL DEV BI ANNUAL REVIEW OF CELL AND DEVELO生物医学32960735-1097J AM COLL CARDIOL JOURNAL OF THE AMERICAN COLLEGE20440169-5347TRENDS ECOL EVOL TRENDS IN ECOLOGY & EVOLUTION生物71041614-6832ADV ENERGY MATER Advanced Energy Materials CHEMISTRY, PHYS 52791097-6256NAT NEUROSCI NATURE NEUROSCIENCE医学化学59481389-5567J PHOTOCH PHOTOBIO C JOURNAL OF PHOTOCHEMISTRY AND P81491946-6234SCI TRANSL MED Science Translational Medicine CELL BIOLOGY医学64501520-765X EUR HEART J SUPPL EUROPEAN HEART JOURNAL SUPPLEM14600066-4197ANNU REV GENET ANNUAL REVIEW OF GENETICS生物39160927-796X MAT SCI ENG R MATERIALS SCIENCE & ENGINEERING 工程技术80491936-122X ANNU REV BIOPHYS Annual Review of Biophysics生物76891759-4758NAT REV NEUROL Nature Reviews Neurology医学21640195-668X EUR HEART J EUROPEAN HEART JOURNAL医学37130896-6273NEURON NEURON医学20380169-409X ADV DRUG DELIVER REV ADVANCED DRUG DELIVERY REVIEWS医学75131748-0132NANO TODAY Nano Today工程技术86088755-1209REV GEOPHYS REVIEWS OF GEOPHYSICS地学81551947-5454ANNU REV CONDEN MA P Annual Review of Condensed Matter Physi PHYSICS, CONDEN 19620167-5729SURF SCI REP SURFACE SCIENCE REPORTS化学11550033-2909PSYCHOL BULL PSYCHOLOGICAL BULLETIN医学5530017-5749GUT GUT医学51931088-9051GENOME RES GENOME RESEARCH生物生物52311092-2172MICROBIOL MOL BIOL R MICROBIOLOGY AND MOLECULAR BIOL83072047-7538LIGHT-SCI APPL Light-Science & Applications OPTICS 76811758-678X NAT CLIM CHANGE Nature climate change ENVIRONMENTAL 57581359-4184MOL PSYCHIATR MOLECULAR PSYCHIATRY医学1820003-990X ARCH GEN PSYCHIAT ARCHIVES OF GENERAL PSYCHIATRY医学3590009-7322CIRCULATION CIRCULATION医学67721549-1676PLOS MED PLOS MEDICINE MEDICINE, GENER 49041063-5157SYST BIOL SYSTEMATIC BIOLOGY生物81041941-1405ANNU REV MAR SCI Annual Review of Marine Science地学73441723-8617WORLD PSYCHIATRY World Psychiatry医学64881523-9829ANNU REV BIOMED ENG ANNUAL REVIEW OF BIOMEDICAL ENG工程技术76901759-4774NAT REV CLIN ONCOL Nature Reviews Clinical Oncology医学58401369-7021MATER TODAY Materials Today工程技术52771097-2765MOL CELL MOLECULAR CELL生物26720302-2838EUR UROL EUROPEAN UROLOGY医学14580066-4170ANNU REV ENTOMOL ANNUAL REVIEW OF ENTOMOLOGY生物65641530-6984NANO LETT NANO LETTERS工程技术19380166-2236TRENDS NEUROSCI TRENDS IN NEUROSCIENCES医学67381545-9993NAT STRUCT MOL BIOL NATURE STRUCTURAL & MOLECULAR BBIOCHEMISTRY & 76941759-5029NAT REV ENDOCRINOL Nature Reviews Endocrinology医学19350166-0616STUD MYCOL STUDIES IN MYCOLOGY生物20150168-6445FEMS MICROBIOL REV FEMS MICROBIOLOGY REVIEWS生物医学7260021-9738J CLIN INVEST JOURNAL OF CLINICAL INVESTIGATION84752168-6106JAMA INTERN MED JAMA Internal Medicine MEDICINE, GENER医学50501073-449X AM J RESP CRIT CARE AMERICAN JOURNAL OF RESPIRATORY68091552-4450NAT CHEM BIOL Nature Chemical Biology生物57731360-1385TRENDS PLANT SCI TRENDS IN PLANT SCIENCE生物14610066-4219ANNU REV MED ANNUAL REVIEW OF MEDICINE医学80481936-0851ACS NANO ACS Nano工程技术医学67671548-5943ANNU REV CLIN PSYCHO ANNUAL REVIEW OF CLINICAL PSYCHO76951759-5045NAT REV GASTRO HEPAT Nature Reviews Gastroenterology & Hepat医学10000027-8874JNCI-J NATL CANCER I JNCI-Journal of the National Cancer Institu ONCOLOGY医学7570022-1007J EXP MED JOURNAL OF EXPERIMENTAL MEDICINE44551001-0602CELL RES CELL RESEARCH生物68181552-5260ALZHEIMERS DEMENT Alzheimers & Dementia医学79881931-3128CELL HOST MICROBE Cell Host & Microbe生物1050002-953X AM J PSYCHIAT AMERICAN JOURNAL OF PSYCHIATRY医学3970010-8545COORDIN CHEM REV COORDINATION CHEMISTRY REVIEWS化学14620066-4227ANNU REV MICROBIOL ANNUAL REVIEW OF MICROBIOLOGY生物770002-7863J AM CHEM SOC JOURNAL OF THE AMERICAN CHEMICA化学84802168-622X JAMA PSYCHIAT JAMA Psychiatry PSYCHIATRY 43000962-8924TRENDS CELL BIOL TRENDS IN CELL BIOLOGY生物19870167-7799TRENDS BIOTECHNOL TRENDS IN BIOTECHNOLOGY工程技术76871759-0876WIRES COMPUT MOL SCI W IREs Computational Molecular Science CHEMISTRY, MUL 65801531-7331ANNU REV MATER RES ANNUAL REVIEW OF MATERIALS RESE工程技术1150003-0007B AM METEOROL SOC BULLETIN OF THE AMERICAN METEOR地学71151616-301X ADV FUNCT MATER ADVANCED FUNCTIONAL MATERIALS工程技术68331554-8627AUTOPHAGY Autophagy生物75871752-0894NAT GEOSCI Nature Geoscience地学医学19310165-6147TRENDS PHARMACOL SCI TRENDS IN PHARMACOLOGICAL SCIEN15560091-6749J ALLERGY CLIN IMMUN JOURNAL OF ALLERGY AND CLINICAL I医学82642041-1723NAT COMMUN Nature communications Biological Science Disciplines 20200168-8278J HEPATOL JOURNAL OF HEPATOLOGY医学化学60491433-7851ANGEW CHEM INT EDIT ANGEWANDTE CHEMIE-INTERNATIONA19030163-8998ANNU REV NUCL PART S ANNUAL REVIEW OF NUCLEAR AND PA物理43630968-0004TRENDS BIOCHEM SCI TRENDS IN BIOCHEMICAL SCIENCES生物14690067-0049ASTROPHYS J SUPPL S ASTROPHYSICAL JOURNAL SUPPLEME地学天文物理14590066-4189ANNU REV FLUID MECH ANNUAL REVIEW OF FLUID MECHANICS24880270-9139HEPATOLOGY HEPATOLOGY医学3600009-7330CIRC RES CIRCULATION RESEARCH医学940002-9297AM J HUM GENET AMERICAN JOURNAL OF HUMAN GENE生物74601744-4292MOL SYST BIOL Molecular Systems Biology生物61981465-6906GENOME BIOL GENOME BIOLOGY BIOTECHNOLOGY 36240890-9369GENE DEV GENES & DEVELOPMENT生物370001-6322ACTA NEUROPATHOL ACTA NEUROPATHOLOGICA医学930002-9270AM J GASTROENTEROL AMERICAN JOURNAL OF GASTROENTE医学61551461-023X ECOL LETT ECOLOGY LETTERS环境科学67111543-592X ANNU REV ECOL EVOL S ANNUAL REVIEW OF ECOLOGY EVOLU生物2530006-4971BLOOD BLOOD医学84252157-1724KIDNEY INT SUPPL Kidney International Supplements UROLOGY & NEPHROLOGY 23760261-4189EMBO J EMBO JOURNAL生物35670887-6924LEUKEMIA LEUKEMIA医学62881471-4906TRENDS IMMUNOL TRENDS IN IMMUNOLOGY医学医学1580003-4967ANN RHEUM DIS ANNALS OF THE RHEUMATIC DISEASES85252211-2855NANO ENERGY Nano Energy CHEMISTRY, PHYS 2400006-3223BIOL PSYCHIAT BIOLOGICAL PSYCHIATRY医学16200098-2997MOL ASPECTS MED MOLECULAR ASPECTS OF MEDICINE医学57341355-4786HUM REPROD UPDATE HUMAN REPRODUCTION UPDATE医学16720105-2896IMMUNOL REV IMMUNOLOGICAL REVIEWS医学79641884-4049NPG ASIA MATER NPG Asia Materials MATERIALS SCIEN 81361943-8206ADV OPT PHOTONICS Advances in Optics and Photonics OPTICS24090265-0568NAT PROD REP NATURAL PRODUCT REPORTS化学85472214-109X LANCET GLOB HEALTH Lancet Global Health PUBLIC, ENVIRON 19010163-7827PROG LIPID RES PROGRESS IN LIPID RESEARCH生物28830360-0564ADV CATAL ADVANCES IN CATALYSIS化学26360301-0082PROG NEUROBIOL PROGRESS IN NEUROBIOLOGY医学29480364-5134ANN NEUROL ANNALS OF NEUROLOGY医学20260168-9525TRENDS GENET TRENDS IN GENETICS生物76911759-4790NAT REV RHEUMATOL Nature Reviews Rheumatology医学7130021-9525J CELL BIOLJOURNAL OF CELL BIOLOGY 生物18980163-7258PHARMACOL THERAPEUT P HARMACOLOGY & THERAPEUTICS 医学66051534-5807DEV CELL DEVELOPMENTAL CELL 生物9990027-8424P NATL ACAD SCI USA PROCEEDINGS OF THE NATIONAL ACA 综合性期刊76111754-2189NAT PROTOC Nature Protocols 生物61911464-7931BIOL REV BIOLOGICAL REVIEWS 生物85402213-2600LANCET RESP MED Lancet Respiratory Medicine RESPIRATORY SYSTEM 14650066-4286ANNU REV PHYTOPATHOL ANNUAL REVIEW OF PHYTOPATHOLOG 生物62141467-5463BRIEF BIOINFORM BRIEFINGS IN BIOINFORMATICS 生物33610742-3098J PINEAL RES JOURNAL OF PINEAL RESEARCH 医学42810960-9822CURR BIOL CURRENT BIOLOGY 生物84242157-1422CSH PERSPECT MED Cold Spring Harbor perspectives in medicin MEDICINE, RESEA 62891471-4914TRENDS MOL MED TRENDS IN MOLECULAR MEDICINE 医学47001043-2760TRENDS ENDOCRIN MET TRENDS IN ENDOCRINOLOGY AND MET 医学47361046-6673J AM SOC NEPHROL JOURNAL OF THE AMERICAN SOCIETY 医学67321545-7885PLOS BIOL PLoS Biology BIOCHEMISTRY &46501040-4651PLANT CELL PLANT CELL 生物47171044-579X SEMIN CANCER BIOL SEMINARS IN CANCER BIOLOGY 医学3220008-5472CANCER RES CANCER RESEARCH 医学83162050-084X ELIFE eLife BIOLOGY 84112155-5435ACS CATAL ACS catalysis CHEMISTRY, PHYSICAL 75601751-7362ISME J ISME Journal 环境科学82692041-6520CHEM SCI Chemical science CHEMISTRY, MULT 2610006-8950BRAIN BRAIN 医学11620033-3190PSYCHOTHER PSYCHOSOM PSYCHOTHERAPY AND PSYCHOSOMAT 医学43460966-842X TRENDS MICROBIOL TRENDS IN MICROBIOLOGY 生物85462213-8587LANCET DIABETES ENDO Lancet Diabetes & Endocrinology ENDOCRINOLOGY 76931759-5002NAT REV CARDIOL Nature Reviews Cardiology 医学26130300-5771INT J EPIDEMIOL INTERNATIONAL JOURNAL OF EPIDEMI 医学58301368-7646DRUG RESIST UPDATE DRUG RESISTANCE UPDATES 医学27240305-1048NUCLEIC ACIDS RES NUCLEIC ACIDS RESEARCH 生物33250737-4038MOL BIOL EVOL MOLECULAR BIOLOGY AND EVOLUTION 生物62311469-221X EMBO REP EMBO REPORTS 生物84392160-3308PHYS REV X Physical Review X PHYSICS, MULTIDISCIPLINAR 32930734-9750BIOTECHNOL ADV BIOTECHNOLOGY ADVANCES 工程技术65391527-8204ANNU REV GENOM HUM G A NNUAL REVIEW OF GENOMICS AND H 生物48421058-4838CLIN INFECT DIS CLINICAL INFECTIOUS DISEASES 医学80501936-1327ANNU REV ANAL CHEM Annual Review of Analytical Chemistry CHEMISTRY, ANAL 18530149-7634NEUROSCI BIOBEHAV R NEUROSCIENCE AND BIOBEHAVIORAL 医学49101063-6706IEEE T FUZZY SYST IEEE TRANSACTIONS ON FUZZY SYSTE 工程技术56991350-9462PROG RETIN EYE RES PROGRESS IN RETINAL AND EYE RESE 医学50971078-0432CLIN CANCER RES CLINICAL CANCER RESEARCH 医学81271943-0264CSH PERSPECT BIOL Cold Spring Harbor perspectives in biology CELL BIOLOGY 81541947-5438ANNU REV CHEM BIOMOL Annual review of chemical and biomolecula CHEMISTRY, APPL 47411047-3211CEREB CORTEX CEREBRAL CORTEX 医学76581757-4676EMBO MOL MED EMBO Molecular Medicine 医学15250084-6597ANNU REV EARTH PL SC ANNUAL REVIEW OF EARTH AND PLAN地学天文85372212-6864PHYS DARK UNIVERSE Physics of the Dark Universe ASTRONOMY & ASTROPHYSI 15260085-2538KIDNEY INT KIDNEY INTERNATIONAL医学17610142-9612BIOMATERIALS BIOMATERIALS工程技术76961759-5061NAT REV NEPHROL Nature Reviews Nephrology医学51771087-0792SLEEP MED REV SLEEP MEDICINE REVIEWS医学41100950-6608INT MATER REV INTERNATIONAL MATERIALS REVIEWS工程技术化学18720161-4940CATAL REV CATALYSIS REVIEWS-SCIENCE AND EN41800955-0674CURR OPIN CELL BIOL CURRENT OPINION IN CELL BIOLOGY生物41120950-9232ONCOGENE ONCOGENE医学31840586-7614SCHIZOPHRENIA BULL SCHIZOPHRENIA BULLETIN医学21910198-6325MED RES REV MEDICINAL RESEARCH REVIEWS医学18490149-5992DIABETES CARE DIABETES CARE医学37140896-8411J AUTOIMMUN JOURNAL OF AUTOIMMUNITY医学71011613-6810SMALL SMALL工程技术21950199-9885ANNU REV NUTR ANNUAL REVIEW OF NUTRITION医学85242211-1247CELL REP Cell reports CELL BIOLOGY37210897-4756CHEM MATER CHEMISTRY OF MATERIALS工程技术1570003-4932ANN SURG ANNALS OF SURGERY医学13180040-6376THORAX THORAX医学10150028-3878NEUROLOGY NEUROLOGY医学62101467-2960FISH FISH FISH AND FISHERIES农林科学56481342-937X GONDWANA RES GONDWANA RESEARCH地学56981350-9047CELL DEATH DIFFER CELL DEATH AND DIFFERENTIATION生物85532223-7755GEOCHEM PERSPECT Geochemical Perspectives GEOCHEMISTRY & 4220012-1797DIABETES DIABETES医学57201354-1013GLOBAL CHANGE BIOL GLOBAL CHANGE BIOLOGY环境科学77831838-7640THERANOSTICS THERANOSTICS MEDICINE, RESEA 61781463-9262GREEN CHEM GREEN CHEMISTRY化学78381863-8880LASER PHOTONICS REV Laser & Photonics Reviews物理62171467-7881OBES REV Obesity Reviews医学2760007-1250BRIT J PSYCHIAT BRITISH JOURNAL OF PSYCHIATRY医学74121741-7007BMC BIOL BMC BIOLOGY生物环境科学15570091-6765ENVIRON HEALTH PERSP ENVIRONMENTAL HEALTH PERSPECTIV11570033-295X PSYCHOL REV PSYCHOLOGICAL REVIEW医学69511568-9972AUTOIMMUN REV AUTOIMMUNITY REVIEWS医学3680009-9147CLIN CHEM CLINICAL CHEMISTRY医学医学3730009-9236CLIN PHARMACOL THER CLINICAL PHARMACOLOGY & THERAPE67011542-3565CLIN GASTROENTEROL H Clinical Gastroenterology and Hepatology医学4390012-8252EARTH-SCI REV EARTH-SCIENCE REVIEWS地学58361369-5266CURR OPIN PLANT BIOL CURRENT OPINION IN PLANT BIOLOGY生物医学27100304-419X BBA-REV CANCER BIOCHIMICA ET BIOPHYSICA ACTA-REV生物11730033-5835Q REV BIOPHYS QUARTERLY REVIEWS OF BIOPHYSICS化学560001-8686ADV COLLOID INTERFAC ADVANCES IN COLLOID AND INTERFAC1990004-3591ARTHRITIS RHEUM-US ARTHRITIS & RHEUMATISM-ARTHRITIS医学78321863-2297SEMIN IMMUNOPATHOL Seminars in Immunopathology医学46731040-9238CRIT REV BIOCHEM MOL CRITICAL REVIEWS IN BIOCHEMISTRY 生物25510277-7037MASS SPECTROM REV MASS SPECTROMETRY REVIEWS物理20130168-3659J CONTROL RELEASE JOURNAL OF CONTROLLED RELEASE医学10210028-646X NEW PHYTOL NEW PHYTOLOGIST生物78421864-5631CHEMSUSCHEM ChemSusChem化学37510903-1936EUR RESPIR J EUROPEAN RESPIRATORY JOURNAL医学27360305-7372CANCER TREAT REV CANCER TREATMENT REVIEWS医学48921062-7995PROG PHOTOVOLTAICS PROGRESS IN PHOTOVOLTAICS工程技术生物42350959-437X CURR OPIN GENET DEV CURRENT OPINION IN GENETICS & DEV68281553-7366PLOS PATHOG PLoS Pathogens生物68291553-7390PLOS GENET PLoS Genetics生物11050031-9007PHYS REV LETT PHYSICAL REVIEW LETTERS物理65491529-1839OPTOMETRY OPTOMETRY OPHTHALMOLOGY4270012-3692CHEST CHEST医学41370952-7915CURR OPIN IMMUNOL CURRENT OPINION IN IMMUNOLOGY医学69851571-0645PHYS LIFE REV Physics of Life Reviews生物81631948-7185J PHYS CHEM LETT The journal of physical chemistry letters CHEMISTRY, PHYS 83212050-7488J MATER CHEM A Journal of Materials Chemistry A CHEMISTRY, PHYS环境科学66791540-9295FRONT ECOL ENVIRON FRONTIERS IN ECOLOGY AND THE ENV环境科学38970926-3373APPL CATAL B-ENVIRON APPLIED CATALYSIS B-ENVIRONMENTA8310022-3417J PATHOL JOURNAL OF PATHOLOGY医学1840003-9942ARCH NEUROL-CHICAGO ARCHIVES OF NEUROLOGY医学64781523-0864ANTIOXID REDOX SIGN ANTIOXIDANTS & REDOX SIGNALING生物82562040-3364NANOSCALE Nanoscale CHEMISTRY, MUL80181933-0219MUCOSAL IMMUNOL Mucosal Immunology医学75341749-5016SOC COGN AFFECT NEUR Social Cognitive and Affective Neuroscienc医学42930962-4929ACTA NUMER ACTA NUMERICA MATHEMATICS80611936-8798JACC-CARDIOVASC INTE JACC. Cardiovascular interventions CARDIAC & CARDI 52851098-3600GENET MED GENETICS IN MEDICINE医学1190003-0090B AM MUS NAT HIST BULLETIN OF THE AMERICAN MUSEUM环境科学84892190-5991J CACHEXIA SARCOPENI Journal of Cachexia Sarcopenia and Muscl MEDICINE, GENER 84762168-6149JAMA NEUROL JAMA Neurology CLINICAL NEUROLOGY医学36210890-8567J AM ACAD CHILD PSY JOURNAL OF THE AMERICAN ACADEMY74131741-7015BMC MED BMC Medicine医学76241755-263X CONSERV LETT Conservation Letters BIODIVERSITY CONSERVATIO物理15070079-6565PROG NUCL MAG RES SP PROGRESS IN NUCLEAR MAGNETIC RE19850167-7659CANCER METAST REV CANCER AND METASTASIS REVIEWS医学医学7860022-202X J INVEST DERMATOL JOURNAL OF INVESTIGATIVE DERMATO28540342-4642INTENS CARE MED INTENSIVE CARE MEDICINE医学42370959-440X CURR OPIN STRUC BIOL CURRENT OPINION IN STRUCTURAL BI生物80601936-878X JACC-CARDIOVASC IMAG JACC. Cardiovascular imaging CARDIAC & CARD33330738-8551CRIT REV BIOTECHNOL CRITICAL REVIEWS IN BIOTECHNOLOG工程技术84792168-6203JAMA PEDIATR JAMA Pediatrics PEDIATRICS42210958-1669CURR OPIN BIOTECH CURRENT OPINION IN BIOTECHNOLOG工程技术74501743-8977PART FIBRE TOXICOL Particle and fibre toxicology TOXICOLOGY生物42960962-8436PHILOS T R SOC B PHILOSOPHICAL TRANSACTIONS OF THNEUROPSYCHOPHARMACOLOGY医学36560893-133X NEUROPSYCHOPHARMACO38530923-7534ANN ONCOL ANNALS OF ONCOLOGY医学15500091-3022FRONT NEUROENDOCRIN FRONTIERS IN NEUROENDOCRINOLOG医学17840144-235X INT REV PHYS CHEM INTERNATIONAL REVIEWS IN PHYSICA化学82261998-0124NANO RES Nano Research化学化学14450065-3055ADV ORGANOMET CHEM ADVANCES IN ORGANOMETALLIC CHEM4410012-9615ECOL MONOGR ECOLOGICAL MONOGRAPHS环境科学17300140-7791PLANT CELL ENVIRON PLANT CELL AND ENVIRONMENT生物7120021-9517J CATAL JOURNAL OF CATALYSIS工程技术11830033-8419RADIOLOGY RADIOLOGY医学11250032-0889PLANT PHYSIOL PLANT PHYSIOLOGY生物50551073-8584NEUROSCIENTIST NEUROSCIENTIST医学57701359-7345CHEM COMMUN CHEMICAL COMMUNICATIONS化学35080884-0431J BONE MINER RES JOURNAL OF BONE AND MINERAL RES医学生物58211367-5931CURR OPIN CHEM BIOL CURRENT OPINION IN CHEMICAL BIOLO8170022-3050J NEUROL NEUROSUR PS JOURNAL OF NEUROLOGY NEUROSUR医学COMPUTER SCIEN 68301553-877X IEEE COMMUN SURV TUT IEEE COMMUNICATIONS SURVEYS AND83942150-7511MBIO mBio MICROBIOLOGY 72461674-2788J MOL CELL BIOL Journal of molecular cell biology CELL BIOLOGY 890002-9165AM J CLIN NUTR AMERICAN JOURNAL OF CLINICAL NUT医学52751096-7176METAB ENG METABOLIC ENGINEERING工程技术14380065-2660ADV GENET ADVANCES IN GENETICS生物51221080-6040EMERG INFECT DIS EMERGING INFECTIOUS DISEASES医学81421944-8244ACS APPL MATER INTER ACS Applied Materials & Interfaces工程技术57651359-6446DRUG DISCOV TODAY DRUG DISCOVERY TODAY医学4230012-186X DIABETOLOGIA DIABETOLOGIA医学21520193-936X EPIDEMIOL REV EPIDEMIOLOGIC REVIEWS医学医学47911053-2498J HEART LUNG TRANSPL JOURNAL OF HEART AND LUNG TRANS50601074-5521CHEM BIOL CHEMISTRY & BIOLOGY生物42360959-4388CURR OPIN NEUROBIOL CURRENT OPINION IN NEUROBIOLOGY医学化学15120079-6786PROG SOLID STATE CH PROGRESS IN SOLID STATE CHEMISTR66181535-9476MOL CELL PROTEOMICS MOLECULAR & CELLULAR PROTEOMIC生物75431750-1326MOL NEURODEGENER Molecular Neurodegeneration医学82652041-210X METHODS ECOL EVOL Methods in ecology and evolution ECOLOGY环境科学62071466-822X GLOBAL ECOL BIOGEOGR GLOBAL ECOLOGY AND BIOGEOGRAPH59381388-9842EUR J HEART FAIL EUROPEAN JOURNAL OF HEART FAILU医学49671066-5099STEM CELLS STEM CELLS医学17070129-0657INT J NEURAL SYST International Journal of Neural Systems工程技术25530278-0046IEEE T IND ELECTRON IEEE TRANSACTIONS ON INDUSTRIAL E工程技术42900962-1083MOL ECOL MOLECULAR ECOLOGY生物21560194-911X HYPERTENSION HYPERTENSION医学19340165-9936TRAC-TREND ANAL CHEM TRAC-TRENDS IN ANALYTICAL CHEMIS化学18990163-7525ANNU REV PUBL HEALTH ANNUAL REVIEW OF PUBLIC HEALTH医学41020950-1991DEVELOPMENT DEVELOPMENT生物医学7220021-9630J CHILD PSYCHOL PSYC JOURNAL OF CHILD PSYCHOLOGY AND物理46661040-8436CRIT REV SOLID STATE CRITICAL REVIEWS IN SOLID STATE AN81451945-4589AGING-US Aging CELL BIOLOGY 59271387-6473NEW ASTRON REV NEW ASTRONOMY REVIEWS地学天文74431743-5390NANOTOXICOLOGY Nanotoxicology医学41970956-5663BIOSENS BIOELECTRON BIOSENSORS & BIOELECTRONICS工程技术11870034-4257REMOTE SENS ENVIRON REMOTE SENSING OF ENVIRONMENT环境科学43220964-6906HUM MOL GENET HUMAN MOLECULAR GENETICS生物64871523-7060ORG LETT ORGANIC LETTERS化学61691462-8902DIABETES OBES METAB DIABETES OBESITY & METABOLISM医学81681949-2553ONCOTARGET Oncotarget Oncogenes 47971053-8119NEUROIMAGE NEUROIMAGE医学24860270-6474J NEUROSCI JOURNAL OF NEUROSCIENCE医学63411474-9718AGING CELL AGING CELL生物72451674-2052MOL PLANT Molecular Plant生物8020022-2593J MED GENET JOURNAL OF MEDICAL GENETICS医学79091874-9399BBA-GENE REGUL MECH Biochimica et Biophysica Acta-Gene Regul生物15320090-3493CRIT CARE MED CRITICAL CARE MEDICINE医学81051941-1413ANNU REV FOOD SCI T Annual review of food science and technoloFOOD SCIENCE & 12760038-6308SPACE SCI REV SPACE SCIENCE REVIEWS地学天文81441945-0877SCI SIGNAL Science Signaling BIOCHEMISTRY & 26820303-4240REV PHYSIOL BIOCH P REVIEWS OF PHYSIOLOGY BIOCHEMIS医学84482162-4011ONCOIMMUNOLOGY OncoImmunology ONCOLOGY生物51631084-9521SEMIN CELL DEV BIOL SEMINARS IN CELL & DEVELOPMENTAL9450025-6196MAYO CLIN PROC MAYO CLINIC PROCEEDINGS医学工程技术57541359-0286CURR OPIN SOLID ST M CURRENT OPINION IN SOLID STATE & M79001873-9946J CROHNS COLITIS Journal of Crohns & Colitis医学64971525-0016MOL THER MOLECULAR THERAPY医学14460065-308X ADV PARASIT ADVANCES IN PARASITOLOGY医学68991560-2745FUNGAL DIVERS FUNGAL DIVERSITY生物51441083-4419IEEE T SYST MAN CY B IEEE TRANSACTIONS ON SYSTEMS MA工程技术81121941-7640CIRC-CARDIOVASC INTE Circulation: Cardiovascular Interventions CARDIAC & CARDI 30630378-7753J POWER SOURCES JOURNAL OF POWER SOURCES工程技术生物33830748-3007CLADISTICS CLADISTICS-THE INTERNATIONAL JOUR生物58771383-5742MUTAT RES-REV MUTAT MUTATION RESEARCH-REVIEWS IN MU7250021-972X J CLIN ENDOCR METAB JOURNAL OF CLINICAL ENDOCRINOLO医学62901471-4922TRENDS PARASITOL TRENDS IN PARASITOLOGY医学61641462-2912ENVIRON MICROBIOL ENVIRONMENTAL MICROBIOLOGY环境科学3240008-6223CARBON CARBON工程技术47121044-3983EPIDEMIOLOGY EPIDEMIOLOGY医学36600893-3952MODERN PATHOL MODERN PATHOLOGY医学18730161-5505J NUCL MED JOURNAL OF NUCLEAR MEDICINE医学68751558-3724POLYM REV Polymer Reviews工程技术67831549-9634NANOMED-NANOTECHNOL Nanomedicine-Nanotechnology Biology an工程技术18760161-6420OPHTHALMOLOGY OPHTHALMOLOGY医学29840370-2693PHYS LETT B PHYSICS LETTERS B物理63151473-0197LAB CHIP LAB ON A CHIP工程技术46281029-8479J HIGH ENERGY PHYS JOURNAL OF HIGH ENERGY PHYSICS PHYSICS, PARTICL10420029-6643NUTR REV NUTRITION REVIEWS医学79501879-6257CURR OPIN VIROL Current Opinion in Virology VIROLOGY 41750954-6820J INTERN MED JOURNAL OF INTERNAL MEDICINE医学76151754-6834BIOTECHNOL BIOFUELS Biotechnology for Biofuels工程技术医学62321469-493X COCHRANE DB SYST REV Cochrane Database of Systematic Reviews36740894-1491GLIA GLIA医学16730105-4538ALLERGY ALLERGY医学74331742-7061ACTA BIOMATER Acta Biomaterialia工程技术46641040-841X CRIT REV MICROBIOL CRITICAL REVIEWS IN MICROBIOLOGY生物76631757-7004WIRES RNA WIRES RNA CELL BIOLOGY工程技术35420885-8993IEEE T POWER ELECTR IEEE TRANSACTIONS ON POWER ELEC51121079-5642ARTERIOSCL THROM VAS ARTERIOSCLEROSIS THROMBOSIS AN医学7830022-1899J INFECT DIS JOURNAL OF INFECTIOUS DISEASES医学2090004-637X ASTROPHYS J ASTROPHYSICAL JOURNAL地学天文28390340-5761ARCH TOXICOL ARCHIVES OF TOXICOLOGY医学42700960-7412PLANT J PLANT JOURNAL生物49631065-9471HUM BRAIN MAPP HUMAN BRAIN MAPPING医学5280016-6731GENETICS GENETICS生物14410065-2776ADV IMMUNOL ADVANCES IN IMMUNOLOGY医学34470820-3946CAN MED ASSOC J CANADIAN MEDICAL ASSOCIATION JOU医学3260008-6363CARDIOVASC RES CARDIOVASCULAR RESEARCH医学11560033-2917PSYCHOL MED PSYCHOLOGICAL MEDICINE医学43190964-4563TOB CONTROL TOBACCO CONTROL医学57881364-0321RENEW SUST ENERG REV R ENEWABLE & SUSTAINABLE ENERGY工程技术医学25140272-6386AM J KIDNEY DIS AMERICAN JOURNAL OF KIDNEY DISEA58371369-5274CURR OPIN MICROBIOL CURRENT OPINION IN MICROBIOLOGY生物环境科学67121543-5938ANNU REV ENV RESOUR ANNUAL REVIEW OF ENVIRONMENT AN81081941-3289CIRC-HEART FAIL Circulation-Heart Failure医学35880889-1591BRAIN BEHAV IMMUN BRAIN BEHAVIOR AND IMMUNITY医学1350003-3022ANESTHESIOLOGY ANESTHESIOLOGY医学47501049-250X ADV ATOM MOL OPT PHY ADVANCES IN ATOMIC MOLECULAR AN物理医学53951180-4882J PSYCHIATR NEUROSCI JOURNAL OF PSYCHIATRY & NEUROSC47961053-5888IEEE SIGNAL PROC MAG IEEE SIGNAL PROCESSING MAGAZINE工程技术57551359-0294CURR OPIN COLLOID IN CURRENT OPINION IN COLLOID & INTE化学31020390-6078HAEMATOLOGICA HAEMATOLOGICA HEMATOLOGY地学天文63551475-7516J COSMOL ASTROPART P JOURNAL OF COSMOLOGY AND ASTRO生物60021420-682X CELL MOL LIFE SCI CMLS-Cellular and Molecular Life Sciences9170024-9297MACROMOLECULES MACROMOLECULES工程技术84962192-2640ADV HEALTHC MATER Advanced healthcare materials工程技术82952046-2441OPEN BIOL Open biology BIOCHEMISTRY &工程技术18870162-8828IEEE T PATTERN ANAL IEEE TRANSACTIONS ON PATTERN ANA54041198-743X CLIN MICROBIOL INFEC CLINICAL MICROBIOLOGY AND INFECT医学84402161-1653ACS MACRO LETT ACS Macro Letters化学62151467-7644PLANT BIOTECHNOL J PLANT BIOTECHNOLOGY JOURNAL生物62121467-3037CURR ISSUES MOL BIOL CURRENT ISSUES IN MOLECULAR BIOL生物65071525-7797BIOMACROMOLECULES BIOMACROMOLECULES化学36340891-5849FREE RADICAL BIO MED FREE RADICAL BIOLOGY AND MEDICIN医学40690947-6539CHEM-EUR J CHEMISTRY-A EUROPEAN JOURNAL化学50391072-4710ARCH PEDIAT ADOL MED ARCHIVES OF PEDIATRICS & ADOLESC医学医学24640269-2813ALIMENT PHARM THER ALIMENTARY PHARMACOLOGY & THER9590025-7974MEDICINE MEDICINE医学12840039-2499STROKE STROKE医学69011560-7917EUROSURVEILLANCE EUROSURVEILLANCE INFECTIOUS DISEASES 66541538-7933J THROMB HAEMOST JOURNAL OF THROMBOSIS AND HAEM医学84262157-6564STEM CELL TRANSL MED Stem cells translational medicine CELL & TISSUE ENGINEERING 15130079-6816PROG SURF SCI PROGRESS IN SURFACE SCIENCE工程技术84982192-8606NANOPHOTONICS-BERLIN N anophotonics NANOSCIENCE &66161535-7163MOL CANCER THER MOLECULAR CANCER THERAPEUTICS医学医学70591600-6135AM J TRANSPLANT AMERICAN JOURNAL OF TRANSPLANTA35210885-3185MOVEMENT DISORD MOVEMENT DISORDERS医学35010883-7694MRS BULL MRS BULLETIN工程技术26690301-9268PRECAMBRIAN RES PRECAMBRIAN RESEARCH地学71071615-4150ADV SYNTH CATAL ADVANCED SYNTHESIS & CATALYSIS化学42180957-9672CURR OPIN LIPIDOL CURRENT OPINION IN LIPIDOLOGY医学81131941-7705CIRC-CARDIOVASC QUAL Circulation: Cardiovascular Quality and Ou CARDIAC & CARDI1320003-2700ANAL CHEM ANALYTICAL CHEMISTRY化学26970304-3835CANCER LETT CANCER LETTERS医学84312158-3188TRANSL PSYCHIAT Translational Psychiatry PSYCHIATRY43740969-2126STRUCTURE STRUCTURE生物78331863-2653BRAIN STRUCT FUNCT Brain Structure & Function医学27410306-2619APPL ENERG APPLIED ENERGY工程技术450001-690X ACTA PSYCHIAT SCAND ACTA PSYCHIATRICA SCANDINAVICA医学17750143-5221CLIN SCI CLINICAL SCIENCE医学57371355-6037HEART HEART医学82852045-2322SCI REP-UK Scientific reports Natural Science Disciplines 47861052-9276REV MED VIROL REVIEWS IN MEDICAL VIROLOGY医学34040749-6419INT J PLASTICITY INTERNATIONAL JOURNAL OF PLASTIC工程技术24600268-960X BLOOD REV BLOOD REVIEWS医学64761522-8517NEURO-ONCOLOGY NEURO-ONCOLOGY医学18600160-4120ENVIRON INT ENVIRONMENT INTERNATIONAL环境科学24800269-9370AIDS AIDS医学2780007-1323BRIT J SURG BRITISH JOURNAL OF SURGERY医学13480043-1354WATER RES WATER RESEARCH环境科学57241354-3784EXPERT OPIN INV DRUG EXPERT OPINION ON INVESTIGATIONA医学7550022-0957J EXP BOT JOURNAL OF EXPERIMENTAL BOTANY生物7450022-0477J ECOL JOURNAL OF ECOLOGY环境科学77031759-9954POLYM CHEM-UK POLYMER CHEMISTRY POLYMER SCIENCE 82141994-0416CRYOSPHERE Cryosphere GEOGRAPHY, PHY 18630160-6689J CLIN PSYCHIAT JOURNAL OF CLINICAL PSYCHIATRY医学67821549-9618J CHEM THEORY COMPUT J ournal of Chemical Theory and Computat化学65401527-8999CHEM REC CHEMICAL RECORD化学61951465-542X BREAST CANCER RES BREAST CANCER RESEARCH ONCOLOGY。
