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优周高低压脉冲电子围栏产品使用说明手册本产品获得国家公安部权威认证本产品由太平洋保险公司承保优周高低压脉冲电子围栏产品使用说明手册前言本系统依照中华人民共和国公共安全行业标准以及上海市公安局技防办关于保安系统的有关规定，本着对客户负责的精神，力图达到较高的性能价格比。
本系统以“阻挡为主，报警为辅”的周界安全理念，园区的电子围栏系统注重整体功能完备，系统可靠安全，配置科学合理。
周界防攀越、阻挡报警系统实现基于同一操作平台上的集中控制和功能集成，从而形成功能更加强大、操作更加简单的安保体系。
为使本系统达到技术成熟，经济实用，安全可靠，质量优良的要求，而且是不间断、全天候式的，请严格按照说明书来操作。
目录一、功能与特点...................................................................... (1)1.1 系统功能...................................................................... (1)1.2 系统特点.......................................................................................................2 二、产品说明...................................................................... . (3)2.1 产品设计依据...................................................................... (3)2.2 产品特性...................................................................... (3)2.3 产品技术参数...................................................................... (4)2.4 控制器主机内部及遥控器说明示意图 (5)2.5控制器主机功能端接线说明 (10)三、电子围栏设计要求...................................................................... (10)3.1安全等级...................................................................... (10)3.2安全性............................................................................................................10 四、电子围栏前端安装...................................................................... ..........12 4.1前端安装方式....................................................................... ..............13 4.2周界围栏安装角度...................................................................... ........15 4.3终端杆及终端杆绝缘子安装 (16)4.4中间杆及中间杆绝缘子安装 (16)4.5收紧器安装...................................................................... .....................17 4.6避雷器安装...................................................................... .....................17 4.7合金线之间的连接...................................................................... .........18 4.8围栏警示牌的安装...................................................................... .. (18)4.9接地...................................................................... ..................................18 4.10地下布线...................................................................... ........................18 4.11常用连线方式...................................................................... ................19 五、电子围栏设计需求分析...................................................................... ....19 5.1电子围拦系统布线示意图. (19)5.2电子围栏系统组成部分 (2)5.3控制端控制方式 (20)5.4电子围栏周界需求分析 (20)六、主机安装与连线...................................................................... .................21 6.1主机安装...................................................................... ............................21 6.2安装配件...................................................................... ............................21 6.3主机与围栏连接图...................................................................... .. (22)七、主机调试与报警试验...................................................................... .........26 7.1通电检查...................................................................... ............................26 7.2报警试验...................................................................... ............................26 八、计算机直控软件安装...................................................................... .........27 九、施工安装规范及安全注意事项. (28)9.1施工安装规范 ..................................................................... ....................28 9.2施工中应注意的其他问题 (2)9十、附录A 脉冲电子围栏案例欣赏 (30)附录B安全注意事项...................................................................... . (32)一、功能与特点高低压系列脉冲主机产品主要有三大功能:1.威慑功能2.阻挡功能3.报警输出。
®意大利倍尔实验室仪器设备符号电磁力恢复：永久磁铁中的线圈，用于实现最精确的称重高精度称重传感器：应变计通过测量电阻的变化来测量变形RS232 数据接口：通过此接口连接天平与打印机和其他 PC 外围设备。
测量单位：选择天平显示重量时所用测量单位计件：此功能对相同的项目进行计数，有一些参考数量可供选择检重：上限和下限可以各自设置，用于计量和配料。
百分比称重功能：计算样本重量相比于参考重量(100%)的百分比值。
累计功能：测量多个项目的累计重量。
显示总计和当前重量动物称重：在不稳定条件(如称量活动的实验动物等)下提供准确读数。
密度测定功能：准确测定固态物体或液体的密度最大负荷(峰值保持)功能：称量一连串重物时保留峰值重量GLP 设置：允许存储天平、项目和用户的识别数据。
日期和时间日期和时间：天平显示当前的日期和时间多语言：天平的菜单中有多种语言可供选择配方：允许设置配方中每种成分的名称和公差。
自动配料悬挂称重：可以在天平的底面装一个配有挂钩的支架蓄电池：天平内自带蓄电池，或可连接可选外置蓄电池使用电池操作：天平可以使用一组 AA 电池。
统计功能：显示最小值(Min)、最大值(Max)、总和(Sum)、平均值(Average)、标准偏差(Standard Deviation)毛重-净重-皮重：此功能用于同时显示毛重、净重和皮重。
数据库：内置数据库，用于存储数据和干燥方法纺织功能：确定线的名称、测量单位：Tex 、Td 、Nm 、Nc 。
干燥方式：提供干燥过程在不同温度下的曲线红外传感器：非接触，调用“去皮”或“打印”功能而不用接触天平触摸显示屏：此技术用户友好、易于使用、直观易懂。
蓝牙接口：可以连接蓝牙设备USB 接口：可连接 U 盘图：实时干燥过程可视化显示图密码：用户配置文件可以用密码保护内存：最多可以存储 5 种不同的干燥方案RECIPE STATRS 232G L P % WEIGH%UNITSLIMITS– +UNDERPCSACCUANIMALADDPEAKDENSITYCLOCK LANGBATTERYAANETLC-HREM-FRN S RECIPESTATUNDERACCULANGBATTERYAANETDATABASETEXTILETOUCHSENSMODESBLUETOOTHUSBATGRAPHNET DATABASETEXTILE TOUCH SENS MODES USB PASSWORDM E M OGRAPH ET DATABASE TEXTILE TOUCHSENSMODES USB PASSWORDM E M OGRAPH ABASETEXTILE TOUCHSENSMODESUSBPASSWORD M E M O6-7半微量天平M5-HPB系列可读性 0,01mg8-9半微量天平HPB和S HPB系列可读性 0,01mg10分析天平M系列可读性 0,0001g11精密天平MW和LW 系列可读性 0,001g12可读天平M和L系列可读性 0,01g13可读天平L系列可读性 0,1g14-15可读天平M5-RB 和RB系列可读性 0,01g-0,1g16-17水分测定仪i-Thermo和M5-Thermo 系列可读性 0,0001g-0,001g-0,01g18 可读天平S系列可读性 0,001g-0,01g-0,1g可选配件19-21水分测定仪4( n = 标准 q = 可选的 s = 仅限某些型号)5全自动内部校准M5-HPB-425i 420,01Ø 800,03± 0,08M5-HPB-625i 620,01Ø 800,03± 0,08M5-HPB-105i 1020,01Ø 800,05± 0,08M5-HPB-1265Di 62/1200,01/0,1Ø 800,03 / 0,1± 0,08 / ± 0,2M5-HPB-2285Di 82/2200,01/0,1Ø 800,04 / 0,1± 0,08/ ± 0,2M5-HPB-22105Di102/2200,01/0,1Ø 800,05 / 0,1± 0,08 / ± 0,2系列 - 可读性 0,01mg - 0,1mg型号最大秤量 (g)可读性[d](mg)秤盘尺寸(mm)重复性 (mg)线性误差 (mg)②②②②②②ADD G L P % WEIGH %UNITS LIMITS – +PCS ANIMAL PEAK DENSITY CLOCK LANG RECIPE STAT G L PUNDER ACCU PEAK CLOCK LANG BATTERY AANET DATABASE TEXTILE TOUCH MODES BLUETOOTH USB USB GRAPH PASSWORD M E M O RECIPE ADD G L P % WEIGH %LIMITS – +PCS ANIMAL PEAK DENSITY CLOCK LANG RECIPESTAT G L PUNDER ACCU CLOCK LANGBATTERY AANET DATABASE TEXTILE STAT BATTERY AANET DATABASE TEXTILE M5-M214Ai 2200,1Ø 800,1± 0,0003M5-M314Ai 3100,1Ø 800,1± 0,0003M5-HPB 414i4100,1Ø 800,1mg 如果重量 ≤ 220g 0,3mg 从 220g 到 310g 0,35mg 从 310g 到 410g ± 0,0005M5-HPB 514i 5100,1Ø 800,1mg 如果重量 ≤ 220g 0,3mg 从 220g 到 310g 0,35mg 从 310g 到 510g ± 0,0005M5-HPB 614i 6100,1Ø 800,1mg 如果重量 ≤ 220g 0,3mg 从 220g 到 310g 0,35mg 从 310g 到 610g± 0,0005ADDRS 232G L % WEIGH %UNITSLIMITS– +PCS ANIMALPEAK DENSITY LC-HR7附件TX-110 串行打印机 (AC030)用于固体和液体的密度工具包(AC002)串行到 USB 转换器(E1002))串行电缆，用于串行输出到打印机或电脑(E743)8 GB U盘(AC020)触摸屏手写笔(AC021)技术数据n 量程漂移(+10 … +30°C): +/– 2ppm/°Cn外形尺寸 长×宽×高(毫米):345×215×345n 称重室尺寸 长×宽×高(毫米):162×171×225n 电源 110-230 Vac, 50/60 Hz:输出 24 V /500 mA / 13 VAn 净重：7,2 千克静电消除器技术数据n 操作方式: 2分钟或连续运作最多8小时n 臭氧浓度: 0-0.05 ppm(距离离子源2 厘米)n 放电时间: 9 秒/5 厘米,13秒/10 厘米n 环境条件: 0-50°C，20-80% 空气湿度(无冷凝)n 交流适配器: 交流电 100-240V，50-60 HzA主要功能页面使称重操作更简单百分比称量移液器校准清晰简单的GLP功能输入静电消除器与天平整合:在实验室日常操作过程中，静电会在多种待称重的样品上聚积。
高一化学知识点汇总高一化学学问点汇总：胶体1.胶体区分于其他分散系的本质特征是分散质颗粒直径大小(1～101 nm)。
2.常见胶体Fe(OH)3胶体、Al(OH)3胶体、血液、豆浆、淀粉溶液、蛋白质溶液、有色玻璃、墨水等。
3.Fe(OH)3胶体的制备方法将饱和FeCl3溶液滴入沸水中，接着加热至体系呈红褐色，停止加热，得Fe(OH)3胶体。
4.胶体的提纯：渗析法胶体的提纯：渗析(用半透膜);胶体与浊液的分别：过滤(滤纸)5.区分胶体和其他分散系的最简便方法是利用丁达尔效应。
6.胶体的应用：胶体的在生活、生产和科研等方面有着重要用途，如常见的有：①盐卤点豆腐②肥皂的制取分别③明矾、Fe2(SO4)3溶液净水④FeCl3溶液用于伤口止血⑤江河入海口形成的沙洲⑥冶金厂大量烟尘用高压电除去⑦土壤胶体中离子的吸附和交换过程，保肥作用;⑧纳米技术与胶体高一化学学问点汇总：化学试验平安(1)做有毒气体的试验时，应在通风厨中进行，并留意对尾气进行适当处理(汲取或点燃等)。
进行易燃易爆气体的试验时应留意验纯，尾气应燃烧掉或作适当处理。
(2)烫伤宜找医生处理。
(3)浓酸撒在试验台上，先用Na2CO3 (或NaHCO3)中和，后用水冲擦干净。
浓酸沾在皮肤上，宜先用干抹布拭去，再用水冲净。
浓酸溅在眼中应先用稀NaHCO3溶液淋洗，然后请医生处理。
(4)浓碱撒在试验台上，先用稀醋酸中和，然后用水冲擦干净。
浓碱沾在皮肤上，宜先用大量水冲洗，再涂上硼酸溶液。
浓碱溅在眼中，用水洗净后再用硼酸溶液淋洗。
(5)钠、磷等失火宜用沙土扑盖。
(6)酒精及其他易燃有机物小面积失火，应快速用湿抹布扑盖。
高一化学学问点汇总：金属及其化合物(1)分类专题：①.含金属阳离子的物质分为金属单质、金属氧化物、金属过氧化物、金属超氧化物、金属氢氧化物、金属无氧酸盐、金属含氧酸盐。
②.金属氧化物分为酸性氧化物(过渡元素的高价态氧化物Mn2O7 、CrO3)、碱性氧化物(CaO、Na2O、Fe2O3 、FeO)和两性氧化物(Al2O3 、ZnO)。
用于空气温湿度测量的标准探头，经济实用、应用广泛。
可以配合手持表、记录器和变送器使用。
HC2-S 有两路模拟量信号输出（0...1V），输出信号可以用于OEM产品或数据采集说明:应用手持表、记录器和变送器，直接或者通过延长线连接。
特点湿度工作范围: 0… 100% rh温度工作范围: -50… 100 ° C(-50… 212 ° F)直径: 15mm (0.6 ")长度: 85mm (3.3 ")外壳和过滤器:聚碳酸酯,黑色探头类型HygroClip® 2湿度传感器ROTRONIC Hygromer®IN-1温度传感器Pt100, 1 / 3 Class B长期稳定性测量范围(可互换探头) 0… 100%rh，-50… 100 ° C精度——标准探± 0.8%rh / ± 0.1 K （温度23 °C，湿度10, 35, 80 %rh三点）精度——高精度探头± 0.5%rh / 0.1 K （温度23 °C，湿度10, 20, 30, 40, 50, 60, 70, 80, 90 % rh九点）精度——客户定制探头± 0.5% rh /0.1K （在-10至70 ° C范围内3个可选温度点，20个可选湿度值）；Airchip再现性< 0.02 %rh / 0.01 K响应时间t63 < 15秒初始化时间< 2秒测量间隔通常1秒(计算功能未启用)工作范围-50…100 °C；0…100 %rh允许风速20 m/s报警测量值报警, 传感器故障和短路报警数据存储2000个记录(%rh/°C,日期/时间)软件ROTRONIC HW4统计功能统计功能探头调节(通过软件) 1点或多点温湿度湿度学参数计算露点/霜点事件日志系统相关事件(FDA, GAMP)用户定义模拟量输出量程可以用户信息由HW4进行管理软件数据处理通过接口电缆设备密码保护功能有传感器诊断（漂移,状态）可设定；出厂缺省设置-关溯源/电子记录溯源/电子记录数据存储2000组数据存储供电/功耗 3.2…5 VDC ±0 % / < 4.5 mA信号输出类型 2 x 0…1 VDC模拟信号输出（标准）0…1 V = 0...100 %rh0…1 V = -50...60 °C数字信号输出（标准）UART = Universal AsynchronousReceiverTransmitter (ASCII) 接口标准UART电压反极性保护接头机械性保护温湿度传感器支架有延长电缆最大长度 5 m探头材质聚碳酸酯过滤器聚乙烯过滤器外壳材质聚碳酸酯尺寸15 x 82 mm重量约9 gFDA/GAMP FDA CFR21 part 11 & GAMP订货号HC2-S。
⾼⼀化学必修2-有机化学知识点归纳⾼⼀化学必修2 有机化学知识点归纳(⼆)⼀、有机物的结构与性质1、官能团的定义：决定有机化合物主要化学性质的原⼦、原⼦团或化学键。
2、常见的各类有机物的官能团，结构特点及主要化学性质(1)烷烃A) 官能团：⽆；通式：C n H 2n +2；代表物：CH 4B) 结构特点：键⾓为109°28′，空间正四⾯体分⼦。
烷烃分⼦中的每个C 原⼦的四个价键也都如此。
C) 化学性质：①取代反应（与卤素单质、在光照条件下），，……。
②燃烧③热裂解 (2)烯烃：A) 官能团：；通式：C n H 2n (n ≥2)；代表物：H 2C=CH 2B) 结构特点：键⾓为120°。
双键碳原⼦与其所连接的四个原⼦共平⾯。
C) 化学性质：①加成反应（与X 2、H 2、HX 、H 2O 等）②加聚反应（与⾃⾝、其他烯烃）③燃烧 (3)炔烃：A) 官能团：—C≡C— ；通式：C n H 2n —2(n ≥2)；代表物：HC≡CHB) 结构特点：碳碳叁键与单键间的键⾓为180°。
两个叁键碳原⼦与其所连接的两个原⼦在同⼀条直线上。
CH 4 + Cl 2CH 3Cl + HCl 光CH 3Cl + Cl 2CH 2Cl 2 + HCl 光CH 4 + 2O 2CO 2 + 2H 2O点燃CH 4C + 2H 2⾼温隔绝空⽓C=C CH 2=CH 2 + HXCH 3CH 2X催化剂CH 2=CH 2 + 3O 22CO 2 + 2H 2O点燃n CH 2=CH 2CH 2—CH 2n催化剂CH 2=CH 2 + H 2OCH 3CH 2OH催化剂加热、加压CH 2=CH 2 + Br 2BrCH 2CH 2BrCCl 4原⼦：—X原⼦团（基）：—OH 、—CHO （醛基）、—COOH （羧基）、C 6H 5— 等化学键：、—C ≡C — C=C 官能团C) 化学性质：（略） (4)苯及苯的同系物：A) 通式：C n H 2n —6(n ≥6)；代表物：B)结构特点：苯分⼦中键⾓为120°，平⾯正六边形结构，6个C 原⼦和6个H 原⼦共平⾯。
HC2检测原理
HC2检测原理是基于聚合酶链式反应（PCR）技术的一种检测方法。
该方法通过检测宫颈刮片中高危型人乳头瘤病毒（HPV）的DNA，进而判断是否存在宫颈癌前病变。
具体操作流程如下：
1.采集宫颈刮片样本，提取其中的DNA。
2.将DNA进行预处理，包括去除其中的RNA、蛋白质等杂质。
3.使用HC2检测仪器进行PCR扩增，将样本中的HPV DNA复制成数百万个拷贝。
4.将扩增产物与荧光探针反应，通过荧光信号测定HPV DNA的存在量。
5.根据荧光信号强度，判断样本中是否存在高危型HPV，从而判断宫颈癌前病变的风险程度。
HC2检测原理具有高灵敏度和高特异性，能够准确地检测宫颈癌前病变。
同时，该方法操作简单、快速，适用于大规模筛查和临床诊断。
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USER’S MANUALPORTABLE HIGH CURRENT TEST SETMODEL NUMBER HC2Version 4.1Phenix Technologies Inc.75 Speicher DriveAccident, Maryland 21520Copyright © Phenix Technologies, Inc.Rev 11/14/2016 nabTABLE OF CONTENTSDESCRIPTION SECTION NUMBERDANGER / GENERAL SAFETY PRECAUTIONSTECHNICAL SPECIFICATIONS 1 DEVICE WARNINGS 2 CONTROLS AND INDICATORS 3BASIC APPROACH TO TESTING 4 CALIBRATION 5PARTS LIST 6PARTS ORDERING INFORMATION 7 RECOMMENDED SPARE PARTS 8 RETURNED MATERIAL 9 ELECTRICAL SCHEMATICS 10 CUSTOMER COMMENTS / SUGGESTIONS 11WARNING !!Complete Grounding of this unit is necessary for the safe operation of this equipment. Disconnect inputs before un-grounding this equipmentGENERAL SAFETY PRECAUTIONSHIGH CURRENT/HIGH VOLTAGEThis equipment is capable of providing POTENTIALLY LETHAL VOLTAGESAND/OR SEVERE BURNS! Improper operation or test practices may result in injury or death to the operator or surrounding personnel.The operation of this test equipment should only be performed by personnel familiar with Electrical Testing and Safety Procedures. The operator of this equipment must be aware of all hazards associated with Electrical Testing. The operator is responsible for himself and others in close proximity of the testing area.Some General Safety Practices for working with Electrical Test Equipment have been listed below for your reference.∙Become familiar with your instrument before performing an actual test∙Know your work area, check that all circuits are de-energized and locked out.∙Never work alone; always work with another qualified worker.∙Mark off entire work area with barriers and warning tape as necessary.∙Make all personnel aware of your testing activities.∙Be aware of dangerous conditions that may arise from energizing a test specimen.∙Never modify test equipment, modifications to equipment could introduce an unknown hazard or hinder a designed-in safety feature.∙DO NOT operate damaged equipment. Remove power, and do not use the equipment until safe operation can be verified by service-trained personnel. Phenix Technologies, Inc. assumes no liability for unsafe or improper use of test equipment.SECTION 1: TECHNICAL SPECIFICATIONSThe HC2 is a portable high current test set built for field and shop use. Designed using the latest technology, the HC2 combines a variable high current output with appropriate controls and instrumentation for testing thermal, magnetic, and solid-state motor overload relays, molded-case circuit breakers, and ground fault trip devices. The HC2 can be used in many other applications requiring a high current source.The HC2 provides a short duration output of 2000 amps through a typical 200 amp molded-case circuit breaker when an instantaneous trip element must be tested. Sufficient current is available for testing time delay characteristics of motor overload relays and molded-case circuit breakers.The unique auto-sensing feature makes the HC2 easy to operate. Sensing leads, which operate on either normally-open or normally-closed (non-energized) devices, are connected to the test object auxiliary or non-energized contacts. The output current level can be easily pre-set. When the output is initiated, the pre-set output current locks on and the timer starts. When the test set senses a change in state of the test object auxiliary contacts (NO to NC or NC to NO), the current shuts off and the timer stops. If set to sense current, unit will shut current off at approximately 10% of range switch setting of currentmeter when test object goes open. For instantaneous trip tests, the memory feature of the currentmeter holds the peak current value until reset by the operator.TECHNICAL DATA AND SPECIFICATIONSINPUT: 120 Volts or 220 Volts (as ordered), 50 Hz or 60 Hz (must be preset)OUTPUT: 0-70 Volts at 25 amperes0-14 Volts at 125 amperes0-7 Volts at 250 amperes0-3.5 Volts at 500 amperesOVERLOAD:Short duration overloads are possible on each tap. The test set is capable ofup to 2000 amps, depending on the impedance of the test circuit.DUTY CYCLE:Continuous at 100%5 minutes ON/15 minutes OFF at 200%1 minute ON/5 minutes OFF at 300%30 seconds ON/5 minutes OFF at 400%INSTRUMENTATION:Currentmeter: 3 1/2 digit LCDRanges: 0-1.999/19.99/199.9/1999 amperesAccuracy: +/- .5%Timer: 6 digit LCD, in cycles or secondsRanges: 0-999999 cycles or 0-9999.99 secondsAccuracy: +/- 0.1% readingDIMENSIONS:21.25" W x 16.75" D x 13.75" H; 93 lbs. (220 V unit; 95 lbs.)(540mm W x 426 mm D x 350 mm H; 42.2 kg) (220 V unit; 43 kg)Cables: 11.5 lbs. (5.2 kg.)OUTPUT LEADS: 5 ft. sense control leads5 ft. high current leads (4/0 and 4 Ga)5 ft. low current leads (10 Ga)SECTION 2: DEVICE WARNINGS1. For safe operation, it is important that the unit be plugged into a properly grounded receptacle.2. This unit is not designed for use in energized circuits.3. Due to leakage current within the solid-state circuitry, it is STRONGLY RECOMMENDED to turn controlpower off when changing the output leads.4. NEVER connect sense jacks to energized contacts or terminals being injected by test unit.SECTION 3: CONTROLS AND INDICATORSHC2 Control Panel-1. MAIN POWER This circuit breaker controls the input power to the control and the power sections.2. OUTPUT This circuit breaker protects the variable transformer.3. F1 This protects the control circuitry and power supplies.4. HC ONWhen pressed, this allows current to flow from the unit (provided that the output is shorted as through a closed breaker). If this output current exceeds 10% of the selected output current range, the output will be maintained when the switch is released. Also, the TIMER will begin to run at this point.5. HC OFF When this is pressed, the output current will cease to flow from the unit.6. JOGThis switch is used when it is desired to preset output current to a specific level. When pressed, the output current will begin to flow as it would when HC ON ispressed. The difference is that the TIMER will not begin running in this mode, and the output current will cease to flow when the switch is released, regardless of its magnitude.12316151213111098754614CONTROLS AND INDICATORSHC2 Control Panel (Cont’d)7.RAISE CURRENT Controls current level from minimum to maximum of output tap when HC ON or JOGis activated. Use with JOG to preset current level through test object.8. RANGE SWITCH Four ranges available are 2 Amp (R84), 20 Amp (R85), 200 Amp (R-69), 2000 Amp(R73)9. CURRENTMETER This displays the RMS value of the output current level of the unit. There are fourranges available:2 Amp 1.99920 Amp 19.99200 Amp 199.92000 Amp 1999WARNING: Please note that there is no correlation between the currentmeterrange switch setting and the rating of the taps.MEM/CONT When set to the CONT (continuous) mode, the meter will continuously update todisplay the output current level. When placed in the MEM (memory) mode, the meterwill retain the highest output current level achieved. If the level again exceeds thisvalue, the meter will be updated. If you are below the highest level, pressing RESETwill update the meter to the present level of output.Please note that the MEM mode of operation will only maintain the reading for a shorttime before the reading starts to decay.10. TRIP/RESET This switch serves several purposes.1) When in CONTACTS mode, the lamp will illuminate to indicate that the non-energized contacts of the test specimen connected to the SENSE INPUTS haschanged states.2) When in CURRENT mode, the lamp will illuminate to indicate that the outputcurrent has dropped below the 10% level.3) When pressed, the TIMER will be reset to zero.4) When the CURRENTMETER MEM/CONT is set to the MEM mode, pressing resetwill release currentmeter from its highest registered reading. If the output currentis not at zero, the present output reading will be maintained until either the outputlevel increases, or the reset is pressed again.5) When illuminated, it must be pressed to reset controls for further operation.11. TIMER The timer will begin counting when HC ON is pressed and the current surpasses the10% level. The timer will stop when the current drops below the 10% level in theSENSE CURRENT mode. When in the SENSE CONTACTS mode, the timer will stopwhen a change in the SENSE INPUTS is detected.The timer can be set to read CYCLES or SECONDS.CONTROLS AND INDICATORS12. SENSE SWITCH This can be set to CONTACTS or CURRENT mode. When placed in CONTACTSmode, the unit will react to a change in condition of the SENSE INPUTS. Upon achange in condition, the TIMER will stop running and the output current will cease toflow. The TRIP lamp will illuminate as an indication to the operator of a change incondition.In CONTACTS mode, sense leads must be properly connected to non-energizedcontacts operated by the test specimen or the timer will not shut off when thetest specimen goes open. If the sense leads are not used, the sense switchmust be in CURRENT position for proper functioning.When the SENSE switch is set to the CURRENT mode, the unit will react to the outputcurrent dropping below 10% of the level of the selected current range.13. SENSE INPUTS These are connected to an auxiliary set of contacts (non-energized) regardless if theyare normally open or closed. When RESET is pressed, the unit will remember itsstate. If the state changes, the unit will react to this change if the SENSE switch is setto CONTACTS.CAUTION: Please do not attempt to connect these to the same set of contactswhich you intend to energize, or energized auxiliary contacts. This may causedamage to the unit.14. CONNECTORS Locations for 3 test connections (7 VAC, 250 A), (70 VAC 25 A), (14 VAC, 125 A).15. COMMON Electrical connection for test cable.16. CONNECTOR Test connection (3.5 VAC, 500 A).SECTION 4: BASIC APPROACH TO TESTINGThe HC2 is basically a continuously variable high current source, the magnitudes of which are dependent on the output tap that is selected. Please refer to the Technical Data andSpecifications for the voltage and current ratings of the taps.The output current is controlled by use of the RAISE CURRENT knob on the control panel. By turning the knob in the clockwise direction, the current is raised. By turning the knob in the counter-clockwise direction the output magnitude is decreased.The RMS value of the output current is displayed on the CURRENTMETER. If the MEM/CONT switch is in the CONT (for CONTinuous), the meter displays the real-time value of the output. If the MEM/CONT switch is in the MEM (for MEMory), the highest value of the output current is displayed.When a breaker is to be tested, the operator may select the SENSE CONTACTS mode or the SENSE CURRENT mode of operation. If an auxiliary set of non-energized contacts are available, the SENSE CONTACTS mode may be selected. By pressing JOG or RESET the state of the contacts (be it normally open or normally closed) are remembered by the unit. The operator may then preset the output current to the desired level by use of the JOG switch. When this is done, by pressing HC ON (and assuming that the current level exceeds 10% of the CURRENTMETER range selected), the current will continue flowing after the switch is released and the TIMER will begin to run.When the contacts connected to the SENSE INPUTS change state, the TIMER will stop running and the output current will cease to flow. The TRIP lamp will illuminate as an indication to the operator. If the MEM/CONT switch is in the MEM mode, the highest output current achieved will be displayed on the CURRENTMETER. The operator should note this level before the readings begin to decay. Pressing RESET will zero the TIMER and CURRENTMETER readings, and prepare the unit for operation again.When the SENSE switch is set to the CURRENT mode, the SENSE INPUTS are not used. This position was meant mainly for single pole breakers in which the SENSE INPUTS cannot be used. In this mode initiation of the test is the same as in SENSE CONTACTS mode. The difference is the completion of the test. When the output current falls below 10% of range (as when the contacts of the breaker open) the TIMER stops running and the output current turns off. The TRIP lamp illuminates as an indication to the operator.SECTION 5: CALIBRATIONTIMER The timer is run from a precision programmable oscillator and should never requirecalibration.CURRENTMETER The calibration of the currentmeter should be checked on a yearly basis. To do this, place the MEM/CONT switch in the CONT position. Place the range switch in the 2AMP range. With nothing connected to the output and the RAISE CURRENT knob inits fully counter-clockwise position, adjust R10 until the CURRENTMETER reads zero.With appropriate test equipment (RMS) raise the output current to 80% of each rangeand adjust the corresponding calibration potentiometer to agree with your standard.SECTION 6: HC-2 PARTS LISTSECTION 7: PARTS ORDERING INFORMATIONReplacement parts are available from Phenix Technologies, Inc.Changes to Phenix Technologies' products are sometimes made to accommodate improved components as they become available, and to give you the benefit of the latest technical improvements developed in our Engineering Department. It is, therefore, important when ordering parts to include the serial number of the unit as well as the part number of the replacement part.When your purchase order is received at our office, a representative of Phenix Technologies will contact you to confirm the current price of the part being ordered. If a part you order has been replaced with a new or improved part, an Applications Engineer will contact you concerning any change in part number.Send orders for replacement parts to:Service DepartmentPhenix Technologies, Inc.75 Speicher DriveAccident, Maryland 21520Ph 1 (301) 746-8118Fax: 1 (301) 895-5570E-mail:*******************SECTION 8: RECOMMENDED SPARE PARTSPhenix Technologies recommends that the customer purchase and stock the following parts for normal maintenance of the unit. The recommended quantity should be sufficient to support the unit during normal operation.If the unit will be operated at an isolated site for an extended period or will be subjected to unusual stresses, a larger quantity of parts should be stocked as spares. In such cases, contact Phenix Technologies for a recommendation.Current prices may be obtained by contacting the Service Department at Phenix Technologies.Part Name ComputerNumber Recommended QuantityLamps, 6.3 V, EAO 1420143 8 EAO Momentary – 1 POLE Switch (SW1, 3, 4, 5) 1860120 1 Binding Post – RED 1351102 1 Binding Post - BLACK 1351100 1 F1 (120V) 3 AG 3 Amp 1603603 1 F1 (220V) 3 AG 1.5 Amp 1603602 1 Current Meter – KNS 3 ½ digit 1506400 1 Timer – Red Lion Sub- Cub – D 1505420 1 Lens – Red EAO 1422150 1 Lens – Green EAO 1422151 1 Lens – Amber EAO 1422152 1SECTION 9: RETURNED MATERIALIf for any reason it should become necessary to return this equipment to the factory, the Service Department of Phenix Technologies, Inc. must be given the following information:Name Plate InformationModel NumberSerial NumberReason for ReturnCause of DefectIf Phenix Technologies, Inc. deems return of the part appropriate, it will then issue an "Authorization for Return." If return is not deemed advisable, other inspection arrangements will be made.NOTE: Material received at this plant without the proper authorization shall be held as"Customer's Property" with no service until such time as the proper steps have been taken.Your cooperation is requested in order to ensure prompt service.SECTION 10: ELECTRICAL SCHEMATICS Drawing Number Description1. 9304510 HC-2 Electrical Schematic (120V)2. 9304511 HC-2 Electrical Schematic (220V)SECTION 11: CUSTOMER COMMENTS/SUGGESTIONSPhenix Technologies made significant efforts to ensure that the materials in this Operator’s Manual are correct. If there are concerns or comments as you have used this information, Phenix Technologies appreciates any feedback.Unit Serial Number:Please return to Phenix Technologies, Engineering Department, 75 Speicher Drive, Accident, MD 21520 USA.Phone:1(301)746-8118,Fax1(301)895-5570,*********************。
RDB和HC2的介绍反向斑点杂交（RDB）工作原理，利用生物素等标记的探针和特意性扩增PCR产物(靶DNA序列)杂交。
但是不同于一般的斑点杂交。
一般的点印迹杂交是靶DNA固定于硝酸纤维素膜或尼龙膜上，标记的探针和靶DNA杂交显色。
这种方法每次杂交反映只能判断待测DNA是否含有某一种探针的同源序列，对于某些基因座位可能含有十几至几十个等位基因(如HLADRB位点或地中海贫血突变基因等)，用这种点杂交方法就会很繁杂，甚至可能做不到。
而RDB正是解决了这一难题。
RDB是先将待用的探针分别点到硝酸纤维素膜或尼龙膜上，每个探针一个点并编上号，再将待测的DNA样本(一般是经PCR特意性扩增的产物，在PCR引物5’端预先进行生物素标记，使扩增产物相应标记有生物素)与之杂交，这样待检样本就会与具有同源序列的探针结合，经洗涤去除未结合的DNA样本，由于待测的DNA样本具有生物素类的标记物，结合了待测DNA的探针点上就带有生物素类的标记物，再经相应的显色反应就能显出杂交信号。
这样一次就可以判断某一基因座位的大部分或全部等位基因，因此利用这样的工作原理还可以用于基因分型、病原体检测、肿瘤研究等。
杂交捕获法（HC2），先用试剂中的RNA与标本中的HPV病毒DNA杂交。
RNA的稳定性是很难保证的。
再用微孔板中的包被抗体捕获RNA-DNA杂交体，通过化学发光进行检测。
由于所检测对象为13种病毒中的一种或几种，RNA-DNA杂交体的抗原性是很难保证的。
由于没有扩增放大，该检测灵敏度低，且无法进行HPV分型。
HC2是唯一获美国FDA认证可用于临床的HPV DNA检测技术,已获欧洲CE及中国SDA认证。
有研究表明HC2检测法能定量地检测到HPV病毒的DNA，具有灵敏度高、特异性好、重复性和客观性强的等优点，无需严格的实验室环境及复杂的实验室技能，无需基因扩增，免除了交叉污染的后顾之忧。
但是HC2是进口试剂，成本高，目前在我国不能用于全面普及筛查。
凯杰digene HC2 HPV DNA检测系统产品用途及售后服务旋转混合器DML3000基因杂交信号放大仪自动恒温加热器digene® HC2 HPV DNA 检测试剂盒凯杰企业管理（上海）有限公司1产品用途1.1产品原理基于杂交捕获二代技术（Hybrid Capture® 2，简称HC2），采用专利全长8,000 个碱基对的RNA混合鸡尾酒探针，结合基因杂交、抗体捕获和化学发光信号放大方法，定性且定量地检测世界卫生组织（WHO）国际癌症研究机构（IARC）最新（迄今）公布的13 种高危型HPV：16、18、31、33、35、39、45、51、52、56、58、59 和68 型。
*HC2检测是目前临床HPV DNA检测领域的金标准，也是子宫颈癌筛查的首选方法。
1.1.1HC2技术原理A.样本DNA双链被释放并分解为DNA单链；B.HC2专利全长8000碱基对的RNA探针与DNA单链结合为RNA-DNA杂交体；C.固定在微孔壁的特异性抗体（一抗）捕获RNA-DNA杂交体；D.携带碱性磷酸酶的特异性抗体（二抗）与RNA-DNA杂交体结合；E.基因信号放大仪检测由碱性磷酸酶催化底物释放的化学发光信号。
1.2临床应用1.2.1子宫颈癌筛查A.高度的阴性预测值，安全地将子宫颈癌筛查间隔延长至3 年，由此降低的检查成本远远高于因采用HPV 检测所增加的费用；B.发现高危型HPV持续阳性而细胞学涂片未见异常的妇女，因这一人群中有20–25%会在四年内发展为CIN III或浸润癌；C.找出高危型阳性的妇女，浓缩高危人群，便于进行有效监控，早期发现宫颈病变。
1.2.2ASCUS 的分流与管理A.把潜在患子宫颈癌风险妇女从低风险妇女中分离出来，合理指导阴道镜的使用，提高诊断率，降低漏诊风险；B.相比复查细胞学，HC2 HPV检测提供了更快捷的结果，准确有效地把HPV 阴性的低风险妇女重新放回常规筛查人群中，减少过度诊断和过度治疗；C.作为细胞病理学实验室质量控制的有效手段，通过HC2 HPV 辅助检测，从病因学角度进行分子水平检测来监测细胞学形态结果。
