姓名班级学号实验日期2014.5.21
科目实验名称Mouse Dissection
合作者指导教师成绩
LAB 10: Mouse Dissection
Introduction:
In biomedical research, animal models are always regarded as indispensable tools. They contribute to the scientific discovery in biology and our understanding of the functions of individual genes, even the mechanism of different diseases. Typically, although mice are different from humankind in size and appearance, they have a distinct genetic similarity. At the same time, mice have an efficient ability to reproduce, so they are important research tools for experiments in the lab.
In this experiment, we will exercise to dissect a mouse, so that we can observe the inside of a mammalian body to identify the female and male mice. Learning and recognizing the anatomical structure of mice, including.
Materials and Methods:
Materials:
Operation plate, Scissor, Forceps, Alcohol cotton, Mouse.
Methods: [we get a male mouse]
Part 1: Observations of external features. Make a table T-1.
1．Having an overhead view, identify the mouse’s head, neck, truck, and tail; observe the dorsal and ventral surfaces. Roughly record what is seen.
2．Observe the thorax which is supported by the rib cage, and the abdomen, and the details of appendages attached to the abdomen.
3．Find the mouth, two external nostrils, two external auditory canals, and anus, which are on the body surface.
4．Have a simply look at the surface of reproductive organ, prepuce, urethral and penis including.
And locate the saclike scrotum; feel for the paired testes in the scrotum. Note the rough
features.
Part 2: Observation of organs and structures inside the mouse. [Open the Ventral Body Cavities, Thoracic Cavity, and Abdominopelvic Cavity in order. We must break the ribs near the attachment to the vertebral column to fold back the upper flaps.] Make a table T-2.
1.Ventral Body Cavities.
a. Make a longitudinal incision through the skin with a dissecting scissor, from the neck to the
preputial opening. Pierce the body wall below the ribs, with the blades angled upward, so
that it won’t damage the internal organs.
b. Pull the sides of the longitudinal incision, and look for the diaphragm. Then make two
2. Thoracic Cavity.
a. After opening, observe the heart and cut the thymus away so that we can observe the heart
clearly.
b. Make sure the location of lung, find the trachea and bronchus. Note the features in table T-2.
3.Abdominopelvic Cavity.
a.Observe the liver which is posterior to the diaphragm, look for the gallbladder.
b.Locate the stomach, and then examine the spleen. Note where the small intestine is, and look
for the pancreas (can secrete insulin). Take photos and record the features.
c.Find the place where small intestine empties into large intestine. Observe the short rectum
which is near to anus, and cecum.
d.Separate the esophagus, dorsal mesentery, and the descending portion of the large intestin
e.
Give the urinary bladder.
e.Find two kidneys, and the urethra connected to them, the ureter start from the central of
kidney.
f.Examine the organs of the reproductive system. Use scissors to open the scrotum to expose
the testis, and then find the coiled epididymis and vas deferens.
g.Roughly observe a mouse of the opposite sex.
Results:
Part 1: table T-1
As we can see, the blood vessels on the ear are apparent, and the penis is wrapped in prepuce. The saclike
