《文献信息检索》综合作业 2要求： 1．通过实习，巩固所学的检索策略及资源类型。
2． 写出所用检索词、 检索表达式及命中篇数并复制粘贴 2 篇在文 挡中。
3．课堂作业评点和分析时，希望积极参与教学互动。
一、医学数据库检索 1、Pubmed 库（1）检索福建中医药大学吴锦忠教授发文情况，分别以题 录（Summary）和文摘（Abstrats）格式下载保存。
检索式：wu jz[AU] AND Fujian University of traditional Chinese Medicine[AD]，共 7 篇。
Summary 格式：1: Wu YB, Zheng LJ, Wu JG, Chen TQ, Yi J, Wu JZ. Antioxidant activities of extract and fractions from receptaculum nelumbinis and related flavonol glycosides. Int J Mol Sci. 2012;13(6):7163-73. Epub 2012 Jun 11. PubMed PMID: 22837685; PubMed Central PMCID: PMC3397517. 2: Zeng PY, Wu JG, Liao LM, Chen TQ, Wu JZ, Wong KH. In vitro antioxidant activities of endophytic fungi isolated from the liverwort Scapania verrucosa. Genet Mol Res. 2011 Dec 20;10(4):3169-79. PubMed PMID: 22194173. 3: Lin XH, Wu YB, Lin S, Zeng JW, Zeng PY, Wu JZ. Effects of volatile components and ethanolic extract from Eclipta prostrata on proliferation and differentiation of primary osteoblasts. Molecules. 2010 Jan 8;15(1):241-50. PubMed PMID: 20110887. 4: Wu YB, Zheng CJ, Qin LP, Sun LN, Han T, Jiao L, Zhang QY, Wu JZ. Antiosteoporotic activity of anthraquinones from Morinda officinalis on osteoblasts and osteoclasts. Molecules. 2009 Jan 23;14(1):573-83. PubMed PMID:119169204. 5: Lin S, Zhang H, Han T, Wu JZ, Rahman K, Qin LP. In vivo effect of casticin on acute inflammation. Zhong Xi Yi Jie He Xue Bao. 2007 Sep;5(5):573-6. PubMed PMID: 17854563. 6: Lin S, Zheng WW, Wu JZ, Zhou LJ, Song YN. [PCR, clone and sequence analysis of rDNA-ITS of Nelumbo nucifera from different geographical origins in China]. Zhongguo Zhong Yao Za Zhi. 2007 Apr;32(8):671-5. Chinese. PubMed PMID: 17608215. 7: Zeng JW, Qian SH, Wu JZ, Yang NY. [Studies on involatile constituents of Mentha haplocalyx]. Zhongguo Zhong Yao Za Zhi. 2006 Mar;31(5):400-2. Chinese. PubMed PMID: 16711426.Abstrats 格式：1. Int J Mol Sci. 2012;13(6):7163-73. Epub 2012 Jun 11. Antioxidant activities of extract and fractions from receptaculum nelumbinis and related flavonol glycosides. Wu YB, Zheng LJ, Wu JG, Chen TQ, Yi J, Wu JZ. Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China; E-Mails: wxsq1@ (Y.-B.W.); zhenglijun1985@ (L.-J.Z.); wjg1419@ (J.-G.W.). The antioxidant activities of ethanolic crude extract (ECE) and its four different solvent sub-fractions (namely, petroleum ether fraction (PEF), ethyl acetate fraction (EAF), n-butanol fraction (BF) and the aqueous fraction (AF) from the receptacles of Nelumbo nucifera Gaertn. (Receptaculum Nelumbinis) were investigated using two in vitro antioxidant assays. BF showed the highest total phenolic content (607.6 mg/g gallic acid equivalents), total flavonoid content (862.7 mg/g rutin equivalents) and total proanthocyanidin content (331.0 mg/g catechin equivalents), accompanied with the highest antioxidant activity compared to other fractions through 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) radical scavenging assays. Five flavonol glycosides, namely hyperoside (1), isoquercitrin (2), quercetin-3-O-β-d-glucuronide (3), isorhamnetin-3-O-β-d-galactoside (4) and syringetin-3-O-β-d-glucoside (5) were isolated from the Receptaculum Nelumbinis. Compounds 2-5 were isolated for the first time from the Receptaculum Nelumbinis. The five isolated flavone glycosides, particularly compounds 1-3, demonstrated significant DPPH and ABTS radical scavenging activity, with IC(50) values of 8.9 ± 0.2, 5.2 ±0.2, 7.5 ±0.1 for DPPH and 114.2 ±1.7, 112.8 ±0.8, 172.5 ±0.7 μ g/mL for ABTS, respectively. These results suggest that Receptaculum Nelumbinis has strong antioxidant potential and may be potentially used as a safe and inexpensive bioactive source of natural antioxidants. PMCID: PMC3397517 PMID: 22837685 [PubMed - in process] 2. Genet Mol Res. 2011 Dec 20;10(4):3169-79. In vitro antioxidant activities of endophytic fungi isolated from the liverwort Scapania verrucosa.2Zeng PY, Wu JG, Liao LM, Chen TQ, Wu JZ, Wong KH. Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, China. We investigated in vitro antioxidant activities of 49 endophytic fungi isolated from the liverwort Scapania verrucosa. Based on morphological and molecular identification, the endophytic fungi isolated were classified into seven genera (Hypocrea, Penicillium, Tolypocladium, Chaetomium, Xylaria, Nemania, and Creosphaeria), all belonging to one family (Xylariaceae). By screening with the 2,2'-azino-di(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) decolorization assay, the ethyl acetate extracts of five endophytic fungi (T7, T21, T24, T32, and T38 strains), which exhibited remarkable Trolox equivalent (TE) antioxidant capacity (ranging from 997.06 to 1248.10 μmol TE/g extract), were selected and their antioxidant capacity was further evaluated by assays for 2,2'-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, hydroxyl radical scavenging, reducing power, and ferrous ion chelating. The ethyl acetate extracts of two endophytic fungi (T24 and T38) were found to have comparable scavenging abilities on both DPPH-free radicals (93.9 and 88.7%, respectively, at 50 μg/mL) and hydroxyl radicals (97.1 and 89.4%, respectively, at 2 mg/mL) when compared with those of the positive controls (ascorbic acid and butylated hydroxytoluene, respectively). Although their reducing powers were similar to that of butylated hydroxytoluene, as indicated by absorbance (0.35 and 0.30 at 50 μg/mL, respectively), only the T38 strain's ethyl acetate extract showed ferrous ion chelating ability (92.9% at 1 mg/mL) comparable to that of the EDTA-2Na control. These endophytic fungi in S. verrucosa are a potential novel source of natural antioxidants. PMID: 22194173 [PubMed - indexed for MEDLINE] 3. Molecules. 2010 Jan 8;15(1):241-50. Effects of volatile components and ethanolic extract from Eclipta prostrata on proliferation and differentiation of primary osteoblasts. Lin XH, Wu YB, Lin S, Zeng JW, Zeng PY, Wu JZ. Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350108, China. Eclipta prostrata, an aromatic plant, is known in Chinese herbal medicine for the treatment of various kidney diseases. In the present study, the volatile components were isolated from the aerial parts of this plant by hydrodistillation and analysed by GC-MS. A total of 55 compounds, which were the major part (91.7%) of the volatiles, were identified by matching mass spectra with a mass spectrum library (NIST 05.L). The main components were as follows: heptadecane (14.78%), 6,10,14-trimethyl-2-pentadecanone (12.80%), n-hexadecanoic acid (8.98%), pentadecane (8.68%), eudesma-4(14),11-diene (5.86%), phytol (3.77%), octadec-9-enoic acid (3.35%), 1,2-benzenedicarboxylic acid diisooctyl ester (2.74%), (Z,Z)-9,12-octadecadienoic acid (2.36%), (Z)-7,11-dimethyl-3-methylene-1,6,10-dodecatriene (2.08%) and (Z,Z,Z)-1,5,9,9-tetramethyl-1,4,7-cycloundecatriene (2.07%). The effects of3volatile components and ethanolic extract from the aerial parts of this plant on the proliferation and differentiation of primary osteoblasts were evaluated by the MTT method and measuring the activity of alkaline phosphatase (ALP activity). Both volatile components and ethanolic extract (1 microg/mL to 100 microg/mL) significantly (p < 0.01) stimulated the proliferation and increased the ALP activity of primary osteoblasts. These results propose that E. prostrata can play an important role in osteoblastic bone formation, and may possibly lead to the development of bone-forming drugs. PMID: 20110887 [PubMed - indexed for MEDLINE] 4. Molecules. 2009 Jan 23;14(1):573-83. Antiosteoporotic activity of anthraquinones from Morinda officinalis on osteoblasts and osteoclasts. Wu YB, Zheng CJ, Qin LP, Sun LN, Han T, Jiao L, Zhang QY, Wu JZ. Department of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian, PR China. wxsq1@ Bioactivity-guided fractionation led to the successful isolation of antiosteoporotic components, i.e. physicion (1), rubiadin-1-methyl ether (2), 2-hydroxy-1-methoxy- anthraquinone (3), 1,2-dihydroxy-3-methylanthraquinone (4), 1,3,8-trihydroxy-2-methoxy- anthraquinone (5), 2-hydroxymethyl-3-hydroxyanthraquinone (6), 2-methoxyanthraquinone (7) and scopoletin (8) from an ethanolic extract of the roots of Morinda officinalis. Compounds 4-8 are isolated for the first time from M. officinalis. Among them, compounds 2 and 3 promoted osteoblast proliferation, while compounds 4, 5 increased osteoblast ALP activity. All of the isolated compounds inhibited osteoclast TRAP activity and bone resorption, and the inhibitory effects on osteoclastic bone resorption of compounds 1 and 5 were stronger than that of other compounds. Taken together, antiosteoporotic activity of M. officinalis and its anthraquinones suggest therapeutic potential against osteoporosis. PMID: 19169204 [PubMed - indexed for MEDLINE] 5. Zhong Xi Yi Jie He Xue Bao. 2007 Sep;5(5):573-6. In vivo effect of casticin on acute inflammation. Lin S, Zhang H, Han T, Wu JZ, Rahman K, Qin LP. Department of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian Province 350003, China. OBJECTIVE: To investigate the anti-inflammatory effect of casticin of Fructus Viticis. METHODS: Observations were made on the anti-inflammatory effect of casticin in the models of acute inflammation in vivo. RESULTS: Casticin inhibited significantly xylene-induced mouse ear edema, egg albumen-induced rat paw edema and acetic acid-induced mouse vascular permeability. CONCLUSION: Casticin has significant anti-inflammatory effect on acute inflammation, which is probably related to the inhibition of the inflammation factors. Casticin is the effective constituent contributing to the4anti-inflammatory effect of Fructus Viticis. PMID: 17854563 [PubMed - indexed for MEDLINE] 6. Zhongguo Zhong Yao Za Zhi. 2007 Apr;32(8):671-5. [PCR, clone and sequence analysis of rDNA-ITS of Nelumbo nucifera from different geographical origins in China]. [Article in Chinese] Lin S, Zheng WW, Wu JZ, Zhou LJ, Song YN. Department of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350003, China. OBJECTIVE: To provide DNA molecular marker for identification of Nelumbo nucifera by exploring the differences of nrDNA-ITS sequence of N. nucifera originated from different habitats. METHOD: To compare nrDNA-ITS base sequence using specific PCR-ITS. RESULT: The completed sequence of ITS and 5.8 S rDNA, and the partial sequences of 18S rDNA and 26S rDNA, totally 750 bp, from N. nucifera were obtained. The differences among N. nucifera from different habitats and from different cultivars were found. CONCLUSION: The method can be used to identify N. nucifera among different species and to distinguish their fakes. It provided the basis for identifying N. nucifera from different geographical regions by comparison of their ITS sequences. PMID: 17608215 [PubMed - indexed for MEDLINE] 7. Zhongguo Zhong Yao Za Zhi. 2006 Mar;31(5):400-2. [Studies on involatile constituents of Mentha haplocalyx]. [Article in Chinese] Zeng JW, Qian SH, Wu JZ, Yang NY. Department of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou, China. zjwlantu@ OBJECTIVE: To promote its comprehensive utilization, the involatile constituents of Mentha haplocalyx were studied systematically. METHOD: The chemical components were isolated and purified by silca gel column chromatography and recrystallization. The chemical structures were elucidated on the basis of physico-chemical properties and spectral data. RESULT: Eight compounds were isolated and identified as: acacetin (I), tilianine (II), linarin (III), n-butyl-beta-D-fructopyranoside (IV), ursolic acid (V), oleanolic acid (VI), beta-sitosterol (VII), daucosterol (VIII). CONCLUSION: Compounds I approximately V were obtained from this plant for the first time. PMID: 16711426 [PubMed - indexed for MEDLINE]（2）检索多动症病因及饮食治疗方面的文献。
