BREWERY WASTEWATER TREATMENT IN DASB REACTOR AT AMBIENT TEMPERATURE
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linity was maintained either at 800 or 1,200 mgIL (as CaC03). The wastewater consisted mainly of carbohydrates and was highly biodegradable. The seed sludge for the reactor was collected from the anaerobic digester of a municipal sewage plant, with initial suspended solids (SS) of 48 gIL, volatile suspended solids (VSS) of 21.6 gIL, specific methanogenic activity of 0.08 gCH4 COD/gVSS . d, and median particle diameter of 0.06 mm. The quantity of the seed was 14.4 gVSSIL.
BREWERY WASTEWATER TREATMENT IN DASB REACTOR AT AMBIENT TEMPERATURE By Yue-Gen Yanl and Joo-Hwa Tay2
Granulation was examined in the 12.3 L upflow anaerobic sludge blanket reactor at the average ambient temperature of 21.8°C. Brewery wastewater with an average chemical oxygen demand (COD) of 2,030 mglL and biochemical oxygen demand of 1,150 mgIL was used as substrate, and anaerobic digested sludge as inoculum. During start-up, the sludge loading rate was gradually increased when 80% of soluble COD removal efficiency was achieved. The granules were initially developed after 4-month operation and fully grown after 6-month operation. At 21.8°C, the influent alkalinity seemed to have played a positive role in the granulation process by improving the biogas release from the sludge, with the appropriate value of 1,200 mgCaCOjL. The granules cultivated ranged from 0.4 mm to 1.5 mm in diameter, with specific methanogenic activity of 0.92 gCH4 -COD/gVSS·d and sludge volume index of 14 mL/g of suspended solids. After granulation, the upflow anaerobic sludge blanket reactor performed stably with soluble COD and biochemical oxygen demand removal efficiencies of 89.1 % and 91.3%, respectively, under the volumetric loading rate of 12.2 gCODIL' d and hydraulic retention time of 4 h.
Analytical Methods
The parameters regarding both reactor performance and sludge behavior were measured. Temperature, influent and effluent pH, alkalinity, COD, SS, and biogas production and its compositions were measured twice weekly, and specific methanogenic activity and particle size of the sludge were measured twice monthly. The pH, COD, biochemical oxygen demand (BODs), SS, and VSS were determined according to the standard methods (Standard 1992). The gas production rate was measured by a wet gas meter (Shinagawa W-NK-0.5). The gas compositions were analyzed by gas chromatography (HP 5890A-GC) with a 2 m long and 3 mm inside diameter packed column (Haye-Sep Q, 80/100 mesh) and thermal conductivity detector with a temperature of 200°C. Helium was used as the carrier gas in the gas chromatography operation with a flowrate of 30 mUmin. The specific methanogenic activity (SMA) of the sludge,
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MATERIALS AND METHODS
Experimental Materials
The UASB reactor for the study was a tower-shaped configuration with a gas-liquid-solids separator installed in the upper compartment (Fig. 1). The inside diameters in the reaction and settling zones were 100 and 140 mm, respectively, and the height was 1,300 mm, providing the effective volume of 12.2 L. Sampling ports were built along the height of the reactor at 120 mm intervals, with one additional port for online temperature monitoring. The experiment was conducted at ambient temperatures ranging from 18.6°C to 25.6°C (21.8 :t 1'soC). Brewery wastewater with nutrients and alkalinity adjustment was used as substrate (Table 1). The ratio of chemical oxygen demand (COD) to nitrogen (N) to phosphorus (P) (COD:N:P) was maintained approximately at 200:5: 1. The influent alkaIRes. Scholar, Div. of Water Resour. and Transp., School of Civ. and Struct. Engrg., Nanyang Techno!. Univ., Singapore, 639798. • 2Prof. and Head, Div. of Water Resour. and Transp., School of Civ. and Struct. Engrg., Nanyang Techno!. Univ., Singapore, 639798. Note. Associate Editor: Oliver J. Hao. Discussion open until November I, 1996. To extend the closing date one month, a written request must be filed with the ASCE Manager of Journals. The manuscript for this technical note was submitted for review and possible publication on July 19, 1994. This technical note is part of the ]ou17uJ1 of Environmental Engineering, Vo!. 122, No.6, June, 1996. ©ASCE, ISSN 0733-9372/96/ 0006-0550-0553/$4.00 + $.50 per page. Technical Note No. 8800.
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