流动注射化学发光测定盐酸丙卡特罗

Determination of doxycyclinehydrochloride molecular imprinting chemiluminescence methodZhou Yanmei 1, Zhang Chengli 1, Lei Jian 2, Ma Tongsen 1, Ma 2 glorious, Su Zhiguo 2, Chao Yang 1College of chemistry and chemical engineering, Henan University 1. Henan Kaifeng 4750042 Chinese Science Institute of process engineering, State Key Laboratory of biochemical engineering, Beijing 100080The study found that in alkaline conditions, doxycycline hydrochloride has obvious enhancement of luminescence reaction Lumino ferricyanide system of ChemistryUsing the molecular imprinting, a new flow injection chemiluminescence method for the analysis of legal alligator doxycycline hydrochloride. Methacrylic acid as the functional monomer,Two glycol methacrylate synthesis of molecularly imprinted polymer of doxycycline hydrochloride as crosslinking agent. The molecularly imprinted polymer for molecular recognitionThe material, use a doxycycline hydrochloride a luminol Potassium Ferricyanide Chemiluminescence System, chemiluminescence analysis with flow injection technique was developed for the determination of chemical, saltAcid doxycycline highly selective molecularly imprinted a flow injection chemiluminescence analysis. The linear range of the method is 9 × 10 ~ 6 × 10_5 g called· rnL ~, the detection limit is 3.2 × 10q g · rrlL ~. Water doxycycline hydrochloride solution on 6.0X10 called G and rnL-1 were analyzed, 9 parallelThe relative standard deviation is 3.5%. Content determination of doxycycline hydrochloride in urine and doxycycline hydrochloride tablets by using this method, the result isSatisfied.Keywords molecularly imprinted polymer; flow injection; chemiluminescence; doxycycline hydrochlorideCLC number: 657.3 document code: A Ⅸ) I:10.3964 / j.issn.1000-0593{2009) 07 -1745-05IntroductionDoxycycline hydrochloride (doxycycline hydrochloride, DC) belongs to the secondGeneration of tetracycline antibiotics, hemolytic streptococcus, Staphylococcus aureus and gram positiveBacteria, Pasteurella multocida, Salmonella, Escherichia coli and other gram negativeInhibition of bacteria have a strong, had wider range of antibacterial, was widely used in livestockAnimal husbandry, aquaculture, part of the DC will be stored in the organism, which mayLead exceed the standard DC drug residues in food, thus causing harm to consumers. Because ofThis study established a rapid, accurate,, DC analysis technology, and to ensure food safetyIt has important scientific significance. At present, the determination method of IX reported in the literature: the mainA chromatographic method '2] [1 [3' 4], spectrometry, fluorescence [s], R61 spectrophotometry, capillary[7 '8] tube electrophoresis, chemiluminescence [gadolinium 10] etc..The experiment found that, under alkaline conditions, DC Lumino and potassium ferricyanide systemThe luminescent reaction has obvious enhancement, but because the method is selective limitSystem, is difficult to be applied in complex samples IX: Determination of content analysis. But the molecular markTrace polymer [11-14] is generally adopted by the template molecules with polymerizable functional monomerElectrostatic interactions, Fan Dehua forces, hydrophobic interaction and hydrogen bonding and other non covalent interactions and can beInverse covalent interaction of host guest complexes, adding initiator and crosslinking agentPolymerization, to prepare polymer, by chemical or physical methods to removeThe template molecule, can be "memory" template molecular structure and functional groups of Rigid PolyComplexes, thus the template molecules exhibit specific molecular recognition ability. The experimentSpecific recognition properties of the template molecule with molecular imprinted polymer and capture capability,The template molecule and coexisting substances in the sample separation, and chemiluminescence detectionMeasurement of chemiluminescence analysis method, so as to make up for the shortcomings of the selective. According toThis, this paper takes DC as the template molecular, methacrylic acid as the functional monomer, ethylene twoAlcohol two methyl acrylate as crosslinking agent: synthesis of IX molecular imprinted polymer, andFrom 74 to 100 Tan MIP particles filled in the glass tube column, packed in flow injectionThe photoelectric emission analyzer shoot multiplication optical window chemical pipe, using DC to RumiSensitization and chemiluminescence reaction of potassium ferricyanide chemical, established the DC moleculeA flow injection chemiluminescence analysis was imprinted chemical, and successfully applied to urine and tabletIn DC determination.The 1 part of the experiment1.1 instruments and reagentsIFFM-E type flow injection chemiluminescence analyzer (Xi'an remex analyzerFor a limited liability company); LabTech-1000 type ultraviolet visible spectrophotometer(Beijing Laib Teco Instrument Co. Ltd.); SKY 100C type constant temperature oscillationDevice (Shanghai Su Kun Industrial Co., Ltd.); KQ-100 type ultrasonic cleaner (KunshanUltrasonic instruments Co., Ltd.). Chemiluminescence flow path as shown in figure 1.Received date: 2008 - 08-10. revision date: 2008-11-20Foundation item: National Natural Science Foundation of China Youth (20506026), national high technology research and development program "863" project(2006AAloz437), 2008 year Henan province government decisionStudy on bidding project (B153) and 2008 year Henan Province Department of education science research project (200813610001) supportedAuthor brief introduction: Zhou Yanmei, female, born in 1970, associate professore-mail:zhouyrn of Henan University College of chemistry and Chemical Engineering (Luciola .ca, zhouymhd (. 163.COITIWanfang Data。

流动注射化学发光法测定微乳液介质中盐酸可卡因朱乾华【摘要】在酸性条件下，盐酸可卡因分子中氮原子被质子化后与阴离子AuCl4-形成离子缔合物，该缔合物被二氯甲烷带入含鲁米诺的氯化十六烷基三甲基铵反胶柬微乳液中，离解出来的AuCl4-立即与鲁米诺产生化学发光，结果表明：发光强度与盐酸可卡因的质量浓度在0．001～10mg·L-1范围内呈线性关系，据此提出了间接测定盐酸可卡因的微乳液介质一流动注射化学发光法。

方法的检出限（3σ）为0．01μg·L-1。

以尿样和血清样品为基体，用标准加入法测定方法的回收率，测得回收率在95．0％～102．0％之间，相对标准偏差（n=5）在1．8％～3．2％之间。

%It was found that in an acidic medium, an ion association complex was formed between AuCl4- and the protonated nitrogen atom of cocaine hydrochloride, and this complex was extracted with dichloromethane and the extract was transfered into a reversed micellar microemulsion prepared by mixing cetyltrimethylammonium chloride （CTAC） and luminol. Then AuCL was released and reacted with luminol to produce chemiluminescence. Based on these facts, a FI-CL method for indirect determination of cocaine hydrochloride was proposed. Linearity range of the working curve was kept between 0. 001-- 10 mg·L-1 , with detection limit （3σ）of 0. 01 μg·L-1. Test for recovery was made by standard addition method using samples of urine and blood serum as matrixes, results of recovery found were in the range of 95.0%0--102.0% with RSD＇s （n=5） in the range of 1.8%-3.2%.【期刊名称】《理化检验-化学分册》【年(卷),期】2011(047)009【总页数】3页(P1052-1054)【关键词】微乳液介质;流动注射;化学发光法;盐酸可卡因【作者】朱乾华【作者单位】长江师范学院化学化工学院,重庆408100【正文语种】中文【中图分类】O657.31盐酸可卡因学名为8甲基3(苯甲酞氧基)8氮杂双环[3,2,1]辛烷2甲酸甲醋盐酸盐,是一种局部麻醉药。

中国乡村医药3 讨论初治涂阳肺结核患者免疫功能受抑制，抗结核分枝杆菌保护免疫能力主要由机体T淋巴细胞免疫功能决定，主要效应细胞为活化的巨噬细胞。

CD4+和CD8+可抑制结核分枝杆菌生长、增殖及持续损伤作用[5]。

肺结核发病初期，由于受到结核分枝杆菌的刺激作用，CD4+和CD4+/CD8+水平显著降低，随病情发展降低幅度逐渐加大[6]。

血清sHLA-G可经过体液循环遍布于全身，尤以外周血、脐带血中表达水平较高[7]。

机体受到病原菌入侵时，血清sHLA-G水平显著升高，可增强对T淋巴免疫细胞活性和功能抑制作用，使T淋巴细胞亚群水平失衡，帮助病毒逃避宿主免疫系统的监视和攻击，使得病原菌可长期潜伏于机体中导致疾病发生，促进肺结核的发生和发展[8]。

血清sHLA-G水平还可以预测初治肺结核患者病变累及范围和临床治疗。

本文结果显示，广泛组血清sHLA-G水平高于不广泛组，提示初治肺结核患者病情越严重、病变累及范围越广，血清sHLA-G水平也越高；治疗后该因子水平有所回落，使得致病菌逃离机体细胞免疫监视和攻击的能力显著降低，增强机体对致病菌的杀伤作用，同时也调节T淋巴细胞亚群水平，以增强自然杀伤细胞和细胞毒T细胞的免疫杀伤活性，增强疗效。

参考文献[1]胡耀，宋锁言，张艳芳，等. 免疫调节治疗对于老年肺结核患者细胞免疫功能的影响研究[J]. 中国医刊，2015，50（10）：94.[2]耿书军，刘建玲，冯玉英，等. 胸腺五肽联合常规抗结核方案治疗复治菌阳肺结核的疗效及对患者免疫功能的影响[J]. 中国现代医学杂志，2015，25（7）：51.[3]徐景杰，杭木兰，赵玮. 孕妇血清、羊水可溶性人白细胞抗原-G与胎膜早破的相关性[J]. 武警医学，2014，25（8）：812. [4]中华医学会. 临床诊疗指南: 结核病分册[M]. 北京: 人民卫生出版社，2005：12.[5]杨朝晖，刘东红，翁明钢，等. 初治涂阳肺结核患者细胞因子变化及生命质量分析[J]. 中国公共卫生，2016，32（2）：230.[6]高智，蒋之，万轲. 初治肺结核患者强化治疗前后检测IL-2、SIL-2R及IL-4的临床意义[J]. 安徽医药，2012，16（2）：215.[7]武丽萍，蔡力，陶剑虹. 人类白细胞相关抗原G在移植免疫中的作用及其研究进展[J]. 实用医院临床杂志，2013，10（1）：159.[8]张星. 肺结核病生物学标志物miRNAs筛选、鉴定及miRNA和MRC1 SNPs与肺结核病易感性的研究[D]. 浙江大学，2014.（收稿：2016-05-10 修回：2016-10-19）（发稿编辑：张戈薇）盐酸丙卡特罗治疗小儿支原体肺炎效果观察周挺敏小儿支原体肺炎临床表现主要为发热、咳嗽、气促和肺部啰音等症状体征，部分患儿会出现喘息或呼吸困难症状，病程可持续2周以上。