CHZ868_LCMS_24724_MedChemExpress

1包装清单产品概述MCE 磁力架是磁珠产品专用配套设备，支持 MCE 全线磁珠类产品，内含强磁磁芯，可实现快速高效的分离。

MCE 磁力架 (200 μL / 2 mL / 15 mL ) 采用独特的三明治槽设计，磁条可抽出，可容纳 200 μL PCR 管，1.5 mL EP 管，2 mL EP 管，15 mL 离心管。

本产品适用于抗体纯化、免疫沉淀 (IP )、免疫共沉淀 (Co-IP )、细胞分选和核酸分离等实验。

2操作说明31. 将装有磁珠悬液的 EP 管/离心管置于磁力架对应的样品孔中，静置数分钟后磁珠被吸附聚集于管壁，溶液恢复澄清。

2. 用移液器或吸管从管底将溶液吸出，或小心倾倒出液体。

3. 抽去磁力条，加入复溶液体，轻缓震荡即可混合均匀，进行下一步操作。

注：磁性分离的时间与磁珠粒径有关，磁珠粒径越小，磁性分离时间越长。

此外，溶液的黏稠程度以及溶液的成分也会对磁性分离时间产生影响。

5注意事项1. 根据实验参数和样品体积不同，可调整磁芯位置或选用不同样式的磁力架。

2. 为减少操作过程中磁珠的损失，请将样品管底端插入磁力架底部的凹槽内。

当磁珠吸附在管壁上后，缓慢倾去上清，或用移液枪吸尽。

3. 由于磁力架有强大的磁场，请远离手机、电脑、手表、起博器、磁铁等易被磁力干扰的物体，尤其是刀具，以免对操作人员造成伤害。

4. 如需同时使用多个磁力架 (≥2 个) ，应分开放置，避免磁场之间产生干扰。

不要把多个磁棒放在一起，以防止夹伤。

5. 请勿与强酸、强碱等腐蚀性溶剂直接接触。

6. 请勿拆卸磁块。

7. 为保护外壳，请勿长时间暴露在阳光和紫外线下。

8. 为保持磁力架磁性，请勿置于高温和强外界磁场环境中。

9. 使用后请及时清洁，妥善放置在干燥环境中。

Magnetic StandMedChemExpress MedChemExpress 400-820-3792 电话: ************ 传真: ************Email: t *********************MCE Hotline: 400-820-3792Contents HY-K0200Magnetic Stand 200 μL-2 mL-15 mL。

Inhibitors, Agonists, Screening Libraries Data SheetBIOLOGICAL ACTIVITY:BFH772 is a potent oral VEGFR2 inhibitor, which is highly effective at targeting VEGFR2 kinase with an IC 50 value of 3 nM.IC50 & Target: IC50: 2.7±0.9 nM (hVEGFR2), 1.5±0.53 μM (mVEGFR2), 1.7±0.36 μM (hVEGFR1), 1.1±0.29 μM (hVEGFR3)[1]In Vitro: BFH772 is highly selective; apart from inhibiting VEGFR2 at 3 nM IC 50, it also targets B–RAF, RET, and TIE–2, albeit with atleast 40–fold lower potency. BFH772 is inactive (IC 50>10 μM; >2 μM for cKIT) against all other tyrosine specific– andserine/threonine–specific protein kinases tested. BFH772 inhibits VEGFR2 with IC 50 of 4.6±0.6 nM in CHO cells. BFH772 inhibits VEGFR2 with IC 50 of 3 nM in HUVEC cells. BFH772 inhibits the ligand induced autophosphorylation of RET, PDGFR, and KIT kinases,with IC 50 values ranging between 30 and 160 nM. BFH772 is selective (IC 50 values >0.5 μM) against the kinases of EGFR, ERBB2,INS–R, and IGF–1R and against the cytoplasmic BCR–ABL kinase. IC 50 of BFH772 (<0.01 nM, n=2) demonstrates that they abrogated VEGF induced proliferation at remarkably low nM concentrations [1].In Vivo: BFH772 at 3 mg/kg orally dosed once per day potently inhibits melanoma growth (by 54–90% for primary tumor and71–96% for metastasis growth) as depicted by treatment to control ratios. Dose–response curves of BFH772 at 0.3, 1, and 3 mg/kg demonstrate that even at the lowest concentrations, this naphthalene–1–carboxamide inhibits VEGF induced tissue weight and TIE–2 levels but only reaches statistical significance at 1 mg/kg and above [1].PROTOCOL (Extracted from published papers and Only for reference)Kinase Assay:[1]In vitro kinase assay is based on a filter binding assay, using the recombinant GST–fused kinase domainsexpressed in baculovirus and purified over glutathione–sepharose, γ–[33P]ATP as the phosphate donor, and poly(Glu:Tyr 4:1) peptide as the acceptor. Each GST–fused kinase is incubated under optimized buffer conditions [20 mM Tris–HCl buffer (pH 7.5), 1–3 mM MnCl 2, 3–10 mM MgCl 2, 3–8 μg/mL poly(Glu:Tyr 4:1), 0.25 mg/mL polyethylene glycol 20000, 8 μM ATP, 10 μM sodium vanadate, 1mM DTT] and 0.2 μCi γ–33P ATP in a total volume of 30 μL in the presence or absence of a test substance for 10 min at ambient temperature. The reaction is stopped by adding 10 mL of 250 mM EDTA. Using a 384–well filter system, half the volume istransferred onto an Immobilon–polyvinylidene difluoride membrane. The membrane is then washed extensively and dried, and scintillation counting is performed. IC 50s for compounds are calculated by linear regression analysis of the percentage inhibition [1].Cell Assay: BFH772 is dissolved in DMSO (10 mM) and stored, and then diluted with appropriate medium before use [1]. [1]DifferentBa/F3 cell lines rendered IL–3 independent by transduction with various constitutively active tyrosine kinases are grown in RPMI 1640 medium containing 10% fetal calf serum. For maintenance of parental Ba/F3 cells, the medium is additionally supplemented with 10 ng/mL interleukin–3 (IL–3). For proliferation assays, Ba/F3 cells are seeded on 96–well plates in triplicates at 10000 cells per well and incubated with various concentrations of compounds for 72 h followed by quantification of viable cells using a resazurin sodium salt dye reduction readout (commercially known as Alamar Blue assay). IC 50s are determined with the XLFit Excel Add–In using a four–parameter dose response model [1].Animal Administration: BFH772 is prepared in PEG200 100% (Mice)[1].Product Name:BFH772Cat. No.:HY-100419CAS No.:890128-81-1Molecular Formula:C 23H 16F 3N 3O 3Molecular Weight:439.39Target:VEGFR Pathway:Protein Tyrosine Kinase/RTK Solubility:DMSO: 7.75 mg/mLBFH772 is dissolved in N–methyl pyrrolidone/polyethylene glycol200 (30:70, v/v) (Rat)[1].[1]Mice[1]Female FVB mice weighing between 18 and 20 g are housed in groups of six. Porous chambers containing VEGF (2 μg/mL) in 0.5 mL of 0.8% w/v agar (containing heparin, 20 U/mL) are implanted subcutaneously in the flank of the mice (n=6 per group). VEGF induces the growth of vascularized tissue around the chamber. This response is dose–dependent and can be quantified by measuring the weight and TIE–2 levels of the tissue. Mice are treated either orally once daily with compounds or vehicle (PEG200 100%, 5 mL/kg) starting4–6 h before implantation of the chambers and continuing for 4 days. The animals are sacrificed for measurement of the vascularized tissues 24 h after the last dose. Tissue weight is taken and then a lysate prepared for TIE–2 ELISA analysis .Rat[1]Catheters are implanted into the femoral artery and vein of na?ve female rats strain OFA for BFH772, and BAW2881, or in the jugular vein and femoral artery in female Sprague–Dawley rats for compounds 4, 9, and 10. Animals are allowed to recover for 96 h and are housed in single cages with free access to food and water throughout the experiment. Female OFA rats received 2.5 mg/kg ofBAW2881 dissolved in ethanol/dimethylisosorbide/polyethylene glycol400/D5W (10/15/35/40 v/v) or 1 mg/kg of BFH772 dissolved in N–methyl pyrrolidone/polyethylene glycol200 (30:70, v/v) via injection into the femoral vein. D5W is glucose 5%/water (v/v). Oral administration: BAW2881 and BFH772 are formulated as a micronized suspension (dissolved/suspended in 0.5% carboxymethyl cellulose in distilled water) and administered by gavage to female OFA rats to deliver a dose of 25 mg/kg for BAW2881 or 3 mg/kg BFH772 (n=4 rats per group). For compounds 4, 9, and 10, female Sprague–Dawley rats at 8 weeks of age received an intraveno References:[1]. Bold G, et al. A Novel Potent Oral Series of VEGFR2 Inhibitors Abrogate Tumor Growth by Inhibiting Angiogenesis. J Med Chem. 2016 Jan 14;59(1):132–46.Caution: Product has not been fully validated for medical applications. For research use only.Tel: 609-228-6898 Fax: 609-228-5909 E-mail: tech@Address: 1 Deer Park Dr, Suite Q, Monmouth Junction, NJ 08852, USA。

陈思羽，饶桂维，王露桦，等. 微波辅助深共熔溶剂提取紫斑牡丹籽总黄酮的工艺优化及其抗氧化活性[J]. 食品工业科技，2024，45（5）：161−168. doi: 10.13386/j.issn1002-0306.2023030318CHEN Siyu, RAO Guiwei, WANG Luhua, et al. Optimization of Microwave-assisted Deep Eutectic Solvent Extraction of Total Flavonoids from Paeonia rockii Seeds and Its Antioxidant Activity[J]. Science and Technology of Food Industry, 2024, 45(5):161−168. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023030318· 工艺技术 ·微波辅助深共熔溶剂提取紫斑牡丹籽总黄酮的工艺优化及其抗氧化活性陈思羽1，饶桂维2, *，王露桦3，盛颖霏2（1.浙江树人学院树兰国际医学院，浙江杭州 310015；2.浙江树人学院交叉科学研究院，浙江杭州 310015；3.浙江树人学院生物与环境工程学院，浙江杭州 310015）摘　要：目的：优化紫斑牡丹籽总黄酮的微波辅助深共熔溶剂提取方法，并探究其体外抗氧化活性。

方法：以低共熔溶剂作为提取溶剂，采用微波辅助技术提取紫斑牡丹籽中的总黄酮。

采用单因素实验以及响应面法进行提取工艺优化。

从DPPH 自由基清除率、羟基自由基清除率进行总黄酮的体外抗氧化活性研究。

结果：以氯化胆碱作为氢键受体，脲作为氢键供体的低共熔溶剂体系下，在摩尔比为1:3，含水量41.80%（V/V ）的深共熔溶剂体系最优，料液比1:19 g/mL ，微波功率为110 W ，微波时间3.00 min ，此时紫斑牡丹籽总黄酮得率为1.76 mg/g 。

溴代十六烷吡啶共振瑞利散射法测定肝素
徐红;黄亚励;刘红
【期刊名称】《化工时刊》
【年(卷),期】2011(025)008
【摘要】基于肝素对溴代十六烷吡啶共振瑞利散射的增强作用,建立了一种测定肝素的新方法.在pH值为5.02～8.3的B-R缓冲溶液中,溴代十六烷吡啶与肝素反应
形成缔合物,使溶液共振瑞利散射(RRs)增强,以475 nm处的灵敏度最高,它对肝素
的检出限(3σ)为0.052 mg/L.研究了适宜的反应条件和影响因素,表明该方法灵敏、稳定、选择性好,用于肝素钠注射液的测定,回收率为96.4％～ 101.0％.
【总页数】3页(P29-30,38)
【作者】徐红;黄亚励;刘红
【作者单位】贵阳医学院化学教研室,贵州贵阳550004;贵阳医学院化学教研室,贵
州贵阳550004;贵阳医学院化学教研室,贵州贵阳550004
【正文语种】中文
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