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VICTOR Nivo Multimode Plate ReaderMultimode DetectionDescriptionThe VICTOR ® Nivo ™ is a high-performance filter-based multimode plate reader system that can be equipped with all major detection technologies – absorbance, luminescence, fluorescence intensity, time-resolved fluorescence, fluorescence polarization, and Alpha. It is a compact, light-weight instrument designed for life science research laboratories performing routine low-throughput assays, or assay development work, and with diverse application requirements. The system’s software has a modern, workflow-oriented user interface that is easy to learn and includes pre-defined application protocols to get users productive quickly . In addition, MyAssays Desktop Standard software is provided for data analysis.Detection TechnologiesThe system incorporates a dynamic wheel system with space for storage of up to 32 filters, providing ready access to filters for a large number of dyes. Filters are exchanged between the inner and outer filter wheels, so any individual filter can serve either excitation or emission lightpaths. As a result, there’s no need to install new filters when switching between assays, and filters can be locked within the system so they can’t be mislaid in the lab – ideal for multi-user environments. When fully-loaded, the filter system provides the flexibility to detect many dyes with better sensitivityand greater cost-effectiveness compared to a monochromator. For absorbance measurements, there is a choice of either a filter- or a spectrometer-based system. Full spectrum absorbance measurements are ultra-fast – 230 to 1000 nm at selectable resolutions (2.0 nm, 5.0 nm, 10 nm) in less than one second per well. The spectrometer system also allows for the detection of a wide range of dyes or measurement of samples with unknown absorbance spectra. The system also features high-performance Alpha laser technology, validated for use with our proprietary AlphaScreen ® and AlphaLISA ® technologies.Key Features• Available in four configurations - standard models include absorbance, luminescence, and fluorescence; option to add time-resolved fluorescence, fluorescence polarization, and/or Alpha • T op and bottom reading of all standard technologies (with the exception of Alpha) for plate formats up to 1536-wells • Compact, lightweight instrument frees-up bench space and is easy to move • Internal dynamic filter wheel system with space for up to 32 filters • For absorbance, choice of filter-based detection for best sensitivity or spectrometer for wavelength flexibility • Time resolved fluorescence certified for use with proprietary LANCE ® and HTRF ® technologies • Enhanced Security software for regulated environments provides technological controls and features that support 21 CFR Part 11 compliance • Laser based Alpha detection capabilities for fast and sensitive Alpha measurements • Browser-based software enables control from a variety of devices – PC, laptop, or tablet • Controllable via network or Wi-Fi to facilitate remote working and data access • Optional dispenser for applications such as fast kinetics, flash luminescence, or dual addition assays • Integrated temperature control and optional gas control unit to keep cells healthy during long term kinetic assaysFor research purposes only. Not for use in diagnostic procedures.For a complete listing of our global offices, visit /ContactUsCopyright ©2020, PerkinElmer, Inc. All rights reserved. PerkinElmer ® is a registered trademark of PerkinElmer, Inc. All other trademarks are the property of their respective owners.58608 PKIPerkinElmer, Inc. 940 Winter StreetWaltham, MA 02451 USA P: (800) 762-4000 or (+1) 203-925-4602*ATP detected by VICTOR Nivo with dispenser。

fluisigm ci single cell auto prep system Fluidigm JUNOfluidigm C1fluidigm BIOMARK HDfluidigm EPIfluidigm CYTOF 2fluidigm ACCESS ARRAYfluidigm C1 单细胞自动制备仪用于mRNA 测序更完整的流程- 首尾相连的流程对单细胞进行全转录组分析最高的通量- 每次运行可前所未有的平行处理96 个单细胞更容易的操作- 直接从单细胞开始，简化的样本制备，只需 3 小时手工操作时间，没有片段化和纯化步骤更节省的成本- 是其他文库制备系统成本的1/8 研究人员正在对转录组进行更深的分析，以揭示细胞发育、代谢和疾病的新机理。

mRNA 测序是一种宝贵的工具，它可以帮助研究者理解，在决定细胞命运的关键时期亚群怎样对信号和其他环境线索做出反应，或者它们何时获得异常表型。

在单细胞中研究这些基因表达类型已经极大地促进了细胞生物学的发展。

可是，绝大多数方法对单细胞分析来说并不现实：它们需要大量的细胞，基于太慢或者产生高度可变结果的复杂流程，并且想要从单细胞产生重大结果又太昂贵。

C1? 单细胞mRNA 测序应用测量基因、等位基因和剪接变体的表达水平比较单细胞和群体的表达谱确定转录起始位点鉴定可能的剪接类型评估后转录活动发现新的转录子和基因融合体Fluidigm 公司用于mRNA 测序的C1?单细胞自动制备系统专门为详细分析多种细胞群体表达谱而设计。

C1?单细胞自动制备系统采用了一种全新的途径，基于创新的微流体技术，使用纳升（nL）级别的反应体积。

只需传统文库制备方法一小部分的成本，就能以最低的样本要求，提供一致的结果。

这种新型、简化的流程利用Clontech? 公司的SMARTer? Ultra Low RNA Input 试剂盒进行cDNA 合成，加上Illumina? 公司的Nextera? XT DNA 样本制备试剂盒，来提供手工操作时间最少、最综合的流程。

Unipolar (1 Ubicación) o 3-Vías Unipolar (Múltiples Ubicaciones)Control de Velocidad de Ventilador SilenciosoNo. de Cat. DDF01-BL 1.5A - 120VCA, 60HzINSTRUCCIONES DE INSTALACIÓNDI-000-DDF01-42AADVERTENCIAS Y PRECAUCIONES• P ARA EVITAR DESCARGA ELÉCTRICA, FUEGO, O MUERTE, INTERRUMPA EL PASO DE ENERGÍA mediante el interruptor de circuito o fusible. ¡Asegúrese que el circuito no este energizado antes de iniciar la instalación!• PARA EVITAR DESCARGA ELÉCTRICA, FUEGO, O MUERTE, este control se debe usar con ventiladores de techo de 120 VCA, carga total máxima de 1.5 Amperios.• Para instalarse y/o usarse de acuerdo con los códigos eléctricos y normas apropiadas.• Si usted no está seguro acerca de alguna de las partes de estas instrucciones, consulte a un electricista.• El control de velocidad del ventilador DDF01 Decora ® no es compatible con interruptores estándar de 3 ó 4 vías. Se debe usar con un remoto digital Decora para controlar ubicaciones múltiples, tal como un DD00R-DL .ADVERTENCIAS Y PRECAUCIONES• El largo máximo del cable del control a todos los remotos instalados no debe exceder de 90m (300 ft).• S ólo para usar con ventiladores de paletas con motor con condensador dividido o monofásico de inducción. Por favor vea las instrucciones del fabricante del ventilador o la etiqueta de capacidad en el motor, para confirmar el tipo. Su uso con otro tipo de motor o equipo, puede causar sobrecalentamiento y/o daño al motor o equipo.• Sólo use un (1) control de velocidad de ventilador por carga. El interruptor(es) encenderá el ventilador en el nivel de velocidad seleccionado en el control.• P ara evitar sobrecalentamiento y posible daño a este producto y otro equipo, no lo instale para controlar un receptáculo, iluminación fluorescente, artefacto operado por motor o transformador u otro que no sea un ventilador de techo apropiado.GARANTÍA LIMITADA POR CINCO AÑOS Y EXCLUSIONESLeviton garantiza al consumidor original de sus productos y no para beneficio de nadie más que este producto en el momento de su venta por Leviton está libre de defectos en materiales o fabricación por un período de cinco años desde la fecha de la compra original. La única obligación de Leviton es corregir tales defectos ya sea con reparación o reemplazo, como opción. Para detalles visite o llame al 1-800-824-3005. Esta garantía es inválida si este producto es instalado inapropiadamente o en un ambiente inadecuado, sobrecargado, mal usado, abierto, abusado o alterado en cualquier manera o no es usado bajo condiciones de operación normal, o no conforme con las etiquetas o instrucciones. No hay otras garantías implicadas de cualquier otro tipo, incluyendo mercadotecnia y propiedad para un propósito en particular pero si alguna garantía implicada se requiere por la jurisdicción pertinente, la duración de cualquiera garantía implicada, incluyendo mercadotecnia y propiedad para un propósito en particular, es limitada a cinco años. Leviton no es responsable pordaños incidentales, indirectos, especiales o consecuentes, incluyendo sin limitación, daños a, o pérdida de uso de, cualquier equipo, pérdida de ventas o ganancias o retraso o falla para llevar a cabo la obligación de esta garantía. Los remedios provistos aquí son remedios exclusivos para esta garantía, ya sea basado en contrato, agravio o de otra manera.ARTWORK PRINT SPECIFICATIONSArtwork Print Specification Sheet Rev A11.eps。

Product Information internet-Link Axio Vert.A1Simply Get All Information from Your CellsAxio Vert.A1Simply Get All informationfrom Your Cells› In Brief›the Advantages›the Applications›the System›technology and details›ServiceAnimationAxio Vert.A1Simply Get All information from Your Cells›in Brief› The Advantages›the Applications›the System›technology and details ›ServiceAxio Vert.A1Simply Get All information from Your Cells›in Brief›the Advantages› The Applications›the System›technology and details ›ServiceAxio Vert.A1Simply Get All information from Your Cells›in Brief›the Advantages› The Applications›the System›technology and details›ServiceKeratinocytis of epidermis - DICHeLa cells - Phase contrast HeLa cells - PlasDICTobacco, protoplasts - BrightfieldEmbryo: nucleus with nucleoli visible in right cell - iHMCHeLa cells - 2-channel fluorescenceAxio Vert.A1Simply Get All information from Your Cells›in Brief›the Advantages›the Applications› The System›technology and details ›Service431251 Microscope• Axio Vert.A1, transmitted light• Axio Vert.A1 FL, transmitted lightand fluorescence• Axio Vert.A1 FL-LED,transmitted light and LED fluorescence2 Objectives• A-Plan• LD A-Plan• LD Plan-NEOFLUAR3 Illumination• Transmitted light: halogen lamp, LED (wave-length 400 to 700 nm, peak at 460 nm)• Reflected light: HBO 50, HBO 100, HXP 120 C,LED modules (wavelength, nm): 365, 385, 420,445, 455, 470, 505, 530, 590, 615, 625 orneutral white: 540 - 580 nmAxio Vert.A1Simply Get All information from Your Cells›in Brief›the Advantages›the Applications› The System›technology and details ›ServiceAxio Vert.A1Simply Get All information from Your Cells›in Brief›the Advantages›the Applications›the System› Technology andDetails›ServiceAxio Vert.A1Simply Get All information from Your Cells›in Brief›the Advantages›the Applications›the System› Technology andDetails›ServiceAxio Vert.A1Simply Get All information from Your Cells›in Brief›the Advantages›the Applications›the System› Technology andDetails›ServiceAxio Vert.A1Simply Get All information from Your Cells›in Brief›the Advantages›the Applications›the System› Technology andDetails›ServiceAxio Vert.A1Simply Get All information from Your Cells›in Brief›the Advantages›the Applications›the System› Technology andDetails›ServiceAxio Vert.A1Simply Get All informationfrom Your Cells›in Brief›the Advantages›the Applications›the System›technology and details› ServiceWe are here for you:/microserviceThe moment technology provides you with a result the first time.This is the moment we work for.// CONFIDENCEMADE By CARL ZEISSAxio Vert.A1Simply Get All information from Your Cells›in Brief›the Advantages›the Applications›the System›technology and details ›Service。

BNI IOL-712-000-K023 BNI IOL-714-000-K023User´s GuideContent1Notes 21.1.About this guide 21.2.Struture of the guide 21.3.Typographical conventions 2Enumerations 2 Actions 2 Syntax 2 Cross references 21.4.Symbols 21.5.Abbreviations 2 2Safety 32.1.Intended use 31.1Installation and startup 32.2.General safety notes 32.3.Resistance to aggressive substances 3Hazardous voltage 3 3Getting started 43.1.Connection overview 43.2.Mechanical connection 43.3.Electrical connection 43.4.IO-Link interface 4Connecting the module 4 Module versions 53.5.Sensor interface 5 4IO-Link interface 64.1.IO-Link data 64.2.Process data / input data 6BNI IOL-712-000-K023 6 BNI IOL-714-000-K023 64.3.Parameter datat / Request data 74.4.Errors 74.5.Events 7 5Technical data 85.1.Dimensions 85.2.Mechanical data 85.3.Electrical data 85.4.Operating conditions 85.5.LED indicators 9Status LED 9 6Appendix 106.1.Product ordering code 106.2.Order information 106.3.Scope of delivery 10Balluff Network Interface / IO-Link BNI IOL-712/714-000-K023 1Notes1.1.About thisguide This guide describes the Balluff Network Interface BNI IOL-712/714-000-K023 for the application as peripheral input module to connect analogue sensors. Hereby it is about an IO-Link device which communicates by means of IO-Link protocol with the superordinate IO-Link master assembly.1.2.Struture of theguide The guide is organized so that the sections build on one another. Section 2: Basic safety information.…….1.3.TypographicalconventionsThe following typographical conventions are used in this guide.Enumerations Enumertions are shown in list form with bullet points:•Entry 1•Entry 2Actions Action instructions are indicated by a preceding triangle. The result of an action is indicated by an arrow.Action instruction 1Action resultAction instruction 2Syntax Numbers:Decimal numbers are shown without additional indicators (e.g. 123),Hexadecimal numbers are shown with the additional indicator hex (e.g. 00hex).Cross references Cross references indicate where additional information on the topic can be found.1.4.Symbols Note, TippThis symbol indicates general notes.NoteThis symbol indicates a security notice which most be observed.1.5.Abbreviations BNI Balluff Network InterfaceI-Port Standard input portDPP Direct parameter pageIOL IO-LinkEMC Electromagnetic compatibilityFE Function groundSPDU Service Protocol Data Unit2Safety2.1.Intended use This guide describes the Balluff Network Interface BNI IOL-712/714-000-K023 for theapplication as peripheral output module to connect analogue sensors. Hereby it is about anIO-Link device which communicates by means of IO-Link protocol with the superordinate IO-Link master assembly.1.1Installation andstartup NoteInstallation and startup are to be performed only by trained specialists. Qualified personnel are persons who are familiar with the installation and operation of the product, and who fulfills the qualifications required for this activity. Any damage resulting from unauthorized manipulation or improper use voids the manufacturer's guarantee and warranty. The Operator is responsible for ensuring that applicable of safety and accident prevention regulations are complied with.2.2.General safetynotes Commissioning and inspectionBefore commissioning, carefully read the operating manual.The system must not be used in applications in which the safety of persons is dependent on the function of the device.Authorized PersonnelInstallation and commissioning may only be performed by trained specialist personnel. Intended useWarranty and liability claims against the manufacturer are rendered void by: -Unauthorized tampering-Improper use-Use, installation or handling contrary to the instructions provided in this operating manualObligations of the Operating CompanyThe device is a piece of equipment from EMC Class A. Such equipment may generate RF noise. The operator must take appropriate precautionary measures. The device may only be used with an approved power supply. Only approved cables may be used.MalfunctionsIn the event of defects and device malfunctions that cannot be rectified, the device must be taken out of operation and protected against unauthorized use.Intended use is ensured only when the housing is fully installed.2.3.Resistance toaggressivesubstances NoteThe BNI modules generally have a good chemical and oil resistance. When used in aggressive media (eg chemicals, oils, lubricants and coolants each in high concentration (ie, low water content)) must be checked prior application-related material compatibility. In the event of failure or damage to the BNI modules due to such aggressive media are no claims for defects.Hazardous voltage NoteDisconnect all power before servicing equipment.NoteIn the interest of product improvement, the Balluff GmbH reserves the right tochange the specifications of the product and the contents of this manual at any time without notice.Balluff Network Interface / IO-Link BNI IOL-712/714-000-K0233 Getting started3.1. ConnectionoverviewFigure 3-1: BNI IOL-…-K0231Status LED: Supply, Communication 2 Label3 IO-Link interface 4Analogue input port3.2. Mechanicalconnection To avoid long, shielded analogue cables, the BNI IOL-712/714-000-K023 modules should be attached to the analogue unit which has to be connected. No further mechanical attachment is required.3.3. ElectricalconnectionThe BNI IOL-712/714-000-K023 modules require no separate supply voltage connection. Power is provided through the IO-Link interface by the superordinate IO-Link Master Assembly.3.4. IO-Link interfaceIO-Link (M12, A-coded, male)Connecting the module Connect the BNI IOL-…-K023 either to an IO-Link Master directly or to an analoguesensor.Connect the male plugs unconnected by using cables.Note:A standard 3 wire sensor cable is used for connection to the host IO-Link master.Note:A shielded 4 wire sensor cable is used for connection to the analog actuator.13423 Getting startedModule versions3.5. Sensor interfaceAnalogue input port (M12, A-coded, female)2) Only in case of BNI IOL-714-000-K023* Depending on the IO-Link master, but max. 2A.Balluff Network Interface / IO-Link BNI IOL-712/714-000-K0234 IO-Link interface4.1. IO-Link dataBNI IOL-712-000-K023BNI IOL-714-000-K0234.2. Process data /input dataBNI IOL-712-000-K023BNI IOL-714-000-K023NoteThe measuring range from 4 - 20 mAmp (for BNI IOL-712-000-K023) will be shown in 16384 steps.NoteThe measuring range from 0 - 10 Volt (for BNI IOL-714-000-K023) will be shown in 16384 steps.Error:0 - current > 4mA 1 – current < 4mA4 IO-Link interface4.3. Parameterdatat / Request data4.4. Errors4.5. EventsBalluff Network Interface / IO-Link BNI IOL-712/714-000-K0235Technical data5.1.DimensionsFigure 5-1: Dimensions BNI IOL-...-K0235.2.Mechanicaldata5.3.Electrical data5.4.Operatingconditions5 Technical data5.5. LED indicatorsFigure 5-2: LED indicatorsStatus LEDBNI IOL-71x-000-K023LED 1: CommunicationLED 2: Supply106 Appendix6.1. Product ordering codeBNI IOL-71x-000-K023Balluff Network InterfaceIO-Link interfaceFunctions712 = Current input 4-20mA 714 = Voltage input 0-10VVersions000 = Standard designMechanical designK023 = Plastic housing, HotmeltBus connection and voltage supply 1xM12 male, 4-poles, external thread Analogue port: 1xM12, female, 4-poles, internal thread6.2. Orderinformation6.3. Scope ofdelivery BNI IOL-…-K023 consists of the following components:∙ IO-Link module ∙ User´s guideBalluff GmbHSchurwaldstrasse 973765 Neuhausen a.d.F. DeutschlandTel. +49 7158 173-0 Fax +49 7158 5010 ******************N r . 920741 E ∙ E d i t i o n B 15 ∙ S u b j e c t t o m o d i f i c a t i o n .。

中考攵习堂堂清•英语参考答案第一篇教材知识梳理第一讲七年级（上）Starter Unit 1-Unit 4 知识梳理词汇拓展1 . pleased pleasure 2.meeting 3. fix st 4.second twice S.fiiendly fiiendship6. Chinese7.dictionanes8.iadios9.watches lO.photos ll.thankftil 12.these 13.those 14.1ias had IS.them then thens 16.he him 17.my niuie IS.player19.helpftil 20.whom whose重点短语1• telephone/phone number 2>in English 3<fiist name4. last name 5<niiddle school 6JD card 7.ask...fb「…8>a set of 9<undei the chanlO.on the sofa ll.m the pencil box e on 13.model plane 14.excuse me重点句型1. What is Fm 2 - Wliafs He's His 3.This is4. How Thaiiks5<Nice meet 6>telephoue/phone number It^sV.call me at 8.Who are TheyTe9. Aie you Yes am FnilOJs this it is it isn f t ll.those her aien f t mine 12>Excuse me Aie these aienl 13.Where aie aie on考点探究针对训练1. one2.it 3>that 4>say 5<tell 6 • speak 7>talk8.and 9.so lO.or ll.but堂清练习1〜5.ACBCC6 〜10.BDACA 11 〜15.DCBBD16 -20.CADBB 21 〜25.ACDAB 26 -30.ADBDC31 • radios 32.looking 33<hers 34>going 35 • family36 • spell 37.cousin 38.teacher 39.1ibiaiy40. numbei 41. a photo of my family 42 - where my model plane is 43.on the chan yours 44>is his last name 45>Thaiik you fbf givmg meI Love My FamilyI am a left- behind child.There are five people in my fanuly.They aie my gfandmothei; my paients, my sister and me.My paients work in a big city m oidei to make money fbi living.So my sistei and I live with my giandniothei in a small village.My giandmother is 75 years old.Last yeai, I was badly ill.I had a fever at nudnight.But it was laimiig heavily outside.Aiid my home was fai fiom the town hospital.My giandniothei was womed about me.So she took me to the town hospital.When we anived at the town hospital, she was too tued to stand.The doctor looked over me and said it was notlmig senous.My gfandmotlier snuled.I love my family.I love my giandniothei more than anyone else.I hope my paients can work in mv hometown.第二讲七年级（上）Unit 5〜Unit 9知识梳理词汇拓展1 • bung 2>moie most 3>inteiested bornig 4<difficulty easy 5 - health healthiei healthiest 6>ielaxlelaxed 7.tomatoes S.fifth 9.eighth lO.miith 11.twentieth 12.small bigger biggest 13」o ng/tall14. sale buy sold 15.musician musical16 • use useless 17<scientist scientific 18>happilyhappmess 19>young/new 20. women重点短语1 • watch TV 2.play comput er games 3.play volleyball4. tlmik about5.a pan of 6>buthday party 7.fbf sure 8.have Chinese lesson 9.on Monday moining lO.fiom • • • to • • •重点句型1 • What like foi breakfast 2>How much are TheyTe3.Can/May help you4.Heie you are S.When is your on Januaiy 6.want to be fat7.How old aie you8.Have a good time 9>My favorite subject 10.Why does Because mteiesting考点探究针对训练1 • C 2.buy some piesents fbi my father 3.D 4」ookfeel 5>smell 6>sounds 7.tastes &Look 9. watches lO.ieadmg ll.see 12.D 13 • C堂清练习1 〜5.ABDDD 6 -10.CACCB H -15.BCCDB16 -20.CADCB 21 〜25.CACBD26 -30.DCBCC31 . favorite 32.boiing 33.habit te 35.December 36.fimslies 37.February 3&ninth39. Wednesday 40<photos 41>asks me about my eating hcibit(s) 42>My daughters favorite sport43. What time does he have lunch 44 • How about going fishing 45 • Its lelaxing fbi me46. bioken 47.another 48.woise 49.safe 5O.ielatives 51 .uncle52 • pionuse 53 - alone 54.feel 55.accept第三讲七年级(下)Unit 1〜Unit 4知识梳理词汇拓展1• smgei 2.wntei 3.cential 4.teeth 5.halves6. duty 7ednvei 8>tmth 9Jife lives 10>nnpoitance ll.quiet noise 12Jucky luckily unlucky13.feeling 14<speaker重点短语1-play chess 2.be good at 3.talk to••- 4.play the piano 5.be good with 6.make friends 7.on weekends/the weekend 8>get up 9>take a shower 10>do (one^) homewoik ll.take the subway 12<nde a bike 13>bem r een-c<and ee- 14>come tme 15>go out 16>make one's bed 17.be strict (with sb.)18.follow the mles重点句型1• Wliat club want to join the swimming club 2.to help with 3<Wliat time does gets up at seven oOock4. either or S.How does go to school takes the bus 6<How fai is it not sure five kilometers7. easy to get to school 8<takes me ten nunutes by bike 9>Don r t anive/be late fbi get/anive to/at school on tune lO.Does have to go to bed he does考点探究针对训练1. A2.C3.D4.D5.B堂清练习1〜5.CBDBC6 〜10.ACCBC 11 〜15.DACCB16 -20.ABCDB 21 〜25.ABCAD 26 -30.ADCBC31 . chess 32.kilometeis 33.villagers 34>teetli 35.never 36.listen 37.quiet38. between 39.quickly40.dream 41.will take the subway to visit 42.Don*t anive late fbi cl ass 43 • is good at telling stones44.practice playing the piano eveiy day 45>unpoitant fbi us to followDeal Toni,Glad to receive youi e- mail.Oui school has made many safety mles fbr us to fbllow.Let me tell you something about them.We aie asked to be caiefiil of electncity when we are usmg it.lt is dangerous to play with fixe, fbi it may cause temble accidents.We should make sure doors and windows are shut befbie we leave.Besides, we are not allowed to swim alone, especially in deep fivers or lakes.If we leally want tof we must go to the swinmung pool with our paients or teachers.Whafs more, following traffic mles is also veiy important.Eveiyone should watch out when crossing the load.Aiid oui school wains us not to play on the road.I thuik these rules aie necessaiy as safety comes fiist.Have a good sununef vacation!Li Wei第四讲七年级（下）Unit 5〜Unit 8知识梳理词汇拓展I• beauty beautifiilly 2.scared 3.AustialianYours, 10>menII. childien 12<wmdy 13>suiuiy 14 • cloudy15 . rainy 16.snowy 17.badly 18. wet 19.warm 2O.Canadian 21 .nonliein 22.European重点短语4 • southern 5.African 6>sleepy asleep 7<Ameiican 8.dangerous safety eftil1 • kind of 2<South Afiica 3>get lost4>be m (great) danger 5<cut down 6 • (be) made of7.iead a newspaper 8>go to the movies 9>eat out 10>take a message Ikon vacation12.police station13. across from 14.ni fiont of 15. go along (the street) 16>tiun liglit/Ieft 17>spend tune 18>eiijoy reading重点句型1 • Wliere are from are from South Africa 2.walk on two legs 3.one of the symbols of4. are you doing watching TV5.How f s the weathei in sunny6. How's it going7.to call me back 8Js there a hospital there is on 9.Go along on youi left10. Wheie's behind the police station考点探究针对训练1. C2.thiough3.across4.over5.B6.takes7.spend8.cost9.pay堂清练习1-5.CDCCB 6 -10.CDADB 11 〜15.BABBD16-20.DABBC 21 〜25.BACBA 26 〜30.ADBCD31.delicious 32.spring 33.across 34.spend35.easily 36.sunny 37>teliuig 38>sleepy 39>clnldien 40.visitois 41<Can I take a message 42 • is cooking fbi me 43 • is next to the post office44., spends a lot of time playing computei games 45.are in great danger 46.people47. happeiiiiig 48>caiefiilly 49.that SO.fbund Sl.fewei 52.but 53.inteiested 54>ieadeis 55>many第五讲七年级(下)Unit 9〜Unit 12知识梳理词汇拓展1• height 2.fat 3.fa nner 4.mice S.peisonal6 • sheep 7.expensive cheaply S.potatoes 9.natiiral lO.suiprised/suiprisiiig11.diffeience diffeiently same 12<gl asses 13<womed 14 - slowly quick重点短语1- a little 2 • in the end 3>take one r s oidei 4>make a wish 5>get popular 6.bung good luck to••- k a cow S.nde a horse 9.feed chickens 10.in the countiyside ll.all in allJ12.be mterested in13. stay up late 14.nm away 15.fly a kite 重16.put up 17,up and down 18,wake—up 点句型1. does she look like long straight black hau2.What would Fm not sure 3<The numbei of 4 • How was was great up S.taught us how to make 6.it r s unpoitant to learn 7.put tued our tents to keep us warm 8.so 9.sleeping near the file lO.not to go考占探究针对训练1 -7.ACDCADC堂清练习1 -5.DCCBB 6 -10.DBCAA 11 〜15.CBCDB16 〜20.BACDA21 〜25.CBDCB 26 -30.DBAAD31. straight 32<answei 33>woriy 34>expensive 35Janguage 36>visitois 37<diffeiently38<inteiested 39>put 40>sheep 4Lis getting popular with the students 42.Did you take somephotos 43.am leaining how to nulk a cow 44.stayed up late to watch the basketball match 45<kind of salad would you likeA Pleasant OutingLast weekend, I went fbi an outmg with my classmates.At 7： 00 m the moining, we met at our school gate.We went to the Noitli Hill Park by bike.On the way, we were so excited that we sang loudly.When we ainved there, we started to clunb the hill at once.We had a picnic on the top of the hill.Aftei that, we walked down the path and had a tei; some guls danced undei the trees and some boys played games happily.We didn't go back until 4： 00 p - m.We were tired but veiy happy.What a pleasant outmg it was!第六讲 八年级（上）Unit 1〜Unit''2知识梳理act 3 • build6.huiigef full7.above & different deadbuilding 13Jittle less 149混做1中 healthily 15.decision2 .Everyone on vacation 3.anything special 4 • How was Eveiythnig 5>decided to go6 Hnw often 考点探究针对训练1. B2.B堂清练习1 〜5.CDBCD6 〜10.DABBA 11 〜15.BDDAB16 〜20.BACAD 21 〜25.DCBBC 26 〜30.BDBAC31 - bored 32.once 33.nmid 34>dentist 35.wait 36>enjoyable 37.activities 38>diffeieiices39. Healthy health 4O.helps 41.tiy oui best to help the children 42>exeicise two oi three times a week43.hardly ever eat junk food 44.decide not to take the subway 45.so beautiful that lots of people go there46 • enough 47>crowded 48<noticed 49. folio wed 50 , Thief 51>tluown 52.only 53<allowed 54 • eve 55>fine第七讲 八年级（上）Unit 3〜Unit 4知识梳理词汇拓展1 • better best 2」o udly quiet louder 3.wiimei won wiminig 4.broke broken 5>rich 6<competitoi competition 7>choice chose chosen 8eieport9 • serve seiva nt IO.create ll.magic magical 12.beautifiil beautifiilly 13.crowd重点短语1 • care about 2.as long as 3 - be diffeient fiom词汇拓展 1 • wondeifiil 2.activities active 4 • boring 5.waiter waitress dyinglO.like ll.eiijoyable unhealthy重点短语 1. quite a few 2.of comse 3<feel like. - . 6.at least 7.juiik food 3 • liaidlv ever JJ 4・ because of S.such as 9.moie than lO.less than 重点句型 1. Where did7. How many hoursgo to the movies once a month4. bring out 5 •the same as 6 • in fact 7>be sinulai to8. so far 9.all kinds of 10.be up to ll.play a role12 • make up 13<fbr example 14.take---seriously 15.be talented in 16」augh at 17.aiound the world 18.singmg competition重点句型1• smarter than 2 • as friendly as 3>moie haid-woikuighaidei 4<do you tliuik 5.the best 6>the most conifbitable 7>tliuik of 8.up to you 考点探究针对训练1 • won 2.beat堂清练习1 〜BBB 6 -10.BBACD 11 -15.CDBBD16 -20.BDACB 21 〜25.CADBB 26 -30.BACAC31 • competition 32.talented 33」oudly 34.service 35>cieative 36.necessary 37>piunaiy 38>onifbitable 39<beautifully 40.choose 4Lplay an impoitant role 42>is up to your father 43 • is the most uiterestmg of all the subjects 44 - is not as difficult as 45<take then kiefs request seriously 46.fail 47<sure48.best 49.iemembei- SO.agree 51 .but 52.more 53.difficult 54>Fmally 55.tioubleThere are thiee shoe stores near mv home.Thev are Fuiikv shoe store. Fanulv shoe store and J J JJSpimg shoe stoie.Funky shoe store has the best shoes.Aiid it has fiiendly seivice.But the shoes there are the most expensive? like Fanuly shoe store best, because it has better shoes than Spimg shoe stoie.lt has the fiiendliest seivice.And the shoes there are cheaper than Funky shoe stoie's.As fbr Spimg shoe store, I don't like it at all.l tlrnik it's the worst store, because it has the worst shoes and service, though the shoes there are the cheapest of the three.第八讲八年级（上）Unit5〜Unit 6 知识梳理词汇拓展1• educatio n educate 2.mean meaning 3.discussion 4.act actoi actressS.disappear appearance6 • became become 7.succeed success successfiilly8. lost lost 9 • simply lO.violm ll.pianist12.science scientific 13<medical 14.begimiuig 15Jmpiovement 16>ownei 重点短语1• find out 2.action movie 3.be famous for 4.diess up 5.be ready to 6.take one's place 7.do a good job 8<giow up 9>be sure about 10.make sure11. be able to 12 - at the beginmiig of 13<wnte down14. have to do with 15>take up 16>agiee with重点句型1 • think of nimd stan d2.plan to 3.expect to leain4. dresses up takes her fathers place 5 - to be grow up 6>wnte amcles send to 7ehave to do with 8.agree with 9<to find out gomg on考点探究针对训练1 • to see 2>him 3.C 4<to succeed 5 - success6.successftil7.Make sure8.make sure that堂清练习1-5.BBABA 6-10.DBDAC 11 〜15.DBDBD16-20.BDADC 21 〜25.ACBDB 26 -30.BDACB31 • education 32<fbreign 33 • happenmg 34.simple35 , medicme 36<begiiming 37>aie going to watch 38<hobbies 39,unlucky 40. washing41. had a discussion about movies 42.is ready to go shopping43 • to take his place to play basketball 44.have to do with health 45.going to take up painting 46<expenence 47 • how 48.starts 49<meal 5O.prepaied 51.delicious 52>made 53>whole/wondeifiil 54>electncity 55<about第九讲八年级（上）Unit 7〜Unit 8知识梳理词汇拓展1 • pollute polluted 2.peaceful 3.factones 4>believable unbelievable 5.shook shaken 6>agieement disagree 7<final in the end 8>salty 9>diggmg dug 10>sandwiches ILtiaditional 12.uavelei 13.celebration Impossibly impossible 15 • fellfallen 16.mixture 17.enviiomnental IS.filled empty重点短语1 • play a part 2.space station 3.over and over again4.hundieds of 5>fall down 6」ook for 7 - niilk shake 8.open up 9 - m one hundred years10.pou I -• into ll.cut up 12.tuin on 13 • a piece of with重点句型1.• live to be2.m 100 years they won't be free3.more crowded fewer trees 4 - be like 5 .play a part6.there are woiking7<theie will be &How milk shake 9 • How much lO.put on11.fbiget to考点探究针对训练1.A2.C3.B堂清练习1〜DBD 6 -10.ACABA 11 -15.BCBCA16-20.BABCD 21 〜25.DACBC 26 -30.CDCAB31. cheese 32 • fill 33>sugai 34.peace 35.tiavelei36 Autiinm 37>seive 38 - holiday 39.dangerous 40>dig 41 .fbf 42.spare 43 - climbed 44>noodle 45>cook 46>find 47<Clunese 48>pionunciations 49>expenences 50>alwaysMy life in the FutureMy life in the ftituie will be coloifiil and meaningfiil.I want to be a good doctor when I glow up.I believe Fil bling my patients health and happiness.In my spare tune, Fil stay with my faniily.Well travel a lot and do lots of sports.Fll also read as many books as I can, for I want to impiove myself.Even if Em busy with my work, I will never give up snidymg.ini sure my dream will come tine because of my hard work and the life in the ftituie will get better and better.第十讲八年级仕）Unit 9〜Unit 10知识梳理词汇拓展1. prepaiation2.examine 3>catches catching caught 4<mvitation inviting S.sadness sadly sadder saddest 6.oiganization oiganized 7.advisesuggestion S.undeistanding undeistood 9.caielesscaieftil caiefiilly 10>sohition 11 .angiily anger 12>answer重点短语1. piepaie for 2<go to the doctor 3>hang out 4<the day before yesterday S.the day after tomonow 6 j ook after 7.nun down S.take a trip 9.keep---to oneself10. look fbiwaid to ll.in half 12.make careless mistake（s）重点句型1. Can you come to Fd love to 2>next time 3 • isn f t leavmg until 4>What*s today 5」ook fbrwaid to healing fiom 6.What will happen 7.give me some advice/suggestionsS.the best time to 9.keep them to themselves 10.sharing a problem cutting it in half 考点探究针对训练1.C2.A3.replied 堂清练习1〜5.CBDCC6 〜10.DBCBC 11 〜15. AC ADC16-20.BDBCD 21〜25.BADCA 26 -30.BABCD31 • organize 32.available 33.accept 34 • ad vice 35.meetmg 36.caieftil37.sol ve38> wallet39.piepaiations 40>enough 41 ooking fbnvaid to heaimg from 42einade some careless mistakes43.didift leave until 10 o r clock 44. prepare for the birthday party 45.1 earn to solve then problems by themselves 46>picking 47.saw 48.weanng49.window 5O.if 5Lcold 52 - took 53<liand54 • saying 55>snuledMy Dream JobWorking as a lepoitei is veiy mterestnig, so Fd like to be a repoitei when I grow up.I must work hard to achieve my dieam.I should work hard at all my subjects, especially Cliuiese.Pm going to wnte aiticles eveiy day and send them to magazines and newspapers fiom now on.Pm gomg to majoi in news m college.Aftei graduation, I want to work at a TV station.As a repoiler, I can go to many beautifiil places and meet many niterestmg people.What fiiii it will be?第一讲八年级（下）Unit 1〜Unit 2知识梳理词汇拓展1 • feet 2<lying lay lain 3.climber 4 - knives5. meant meaiiuig 6<impoitant 7.bieath 8<satisfied satisfy9>ownei 10<excitmg excited ll.kind mteiestmg inteiested重点短语1 • have a cold 2>have a stomachache 3」ie down4. take one's temperaune 5<liave a fever 6>take bieaks/a break 7>get off 8>to one's suipiise 9>nght away 10.get into ILbe/get used to 12<take nsks/a nsk 13>iiin out (of)14.cut off IS.get out of16. be in control of 17<give up 18.clean up19. cheer up 20>give out 21>come up with 22>put off 23ehand out 24>call up 25>caie fbi 26.tiy out27.fix up 28>give away 29.take after 30.set up 31 .make a difference重点句型1• Whafs the matter I have a sore back 2.see a dentist get an X-iay 3 - without thinking twice 4>to his suipiise agreed to 5>used to taking risks 6>woiks to help 7.herself the age of &make it possible to have考点探究针对训练1- has a fever 2.has learned 3」ive 4」iving 5.B6. C 7>takes after 8>takes/looks aftei/like 9.D lOehas run out of ll.C堂清练习1〜5.DBCAD 6 -10.BBCCC 11 〜15.DDBBD16-20.BDACB 21 〜25.BCABD 26 -30.CADCB31 • volunteers 32.headache 33 • unagine 34>ownei35.passenger 36<feelings 37<kuidness 38>difficulty39.impoitance 40.decision 41.wants to set up a school 42<volunteeiuig to help the disabled43.without having a lest 44.have difficulty (in) making decisions 45.To my suipiise 46>tiouble 47.idea 48. light 49.befbie SO.angiy SLieftised 52.stolen 53.as 54<sad 55>givmgHow to Keep HealthyKeeping in good health is veiy impoitant m our daily life.But some people don't have a balanced diet.They often stay up late.Aiid they hardly ever exeicise.These bad habits make them unliealthy and always get sick.T hese bad habits are bad fbi theu work and study •I thuik we must pay attention to oui health.We should sleep well so that we can have enough energy to work and study.And it's necessaiy to have a balanced diet.At last, we have to do moie exercise in oui sp are tune.If we keep doing these, we will be strong enough •Remembei our health is moie impoitant than money.Oui greatest wealth is health •第十二讲八年级。

1.AWG1.1.StsEnableAWG ()StsEnableAWG (Floating VI，……);ParametersVI填写需要使能的VI源名称，此处VI源只能填FPVI、FOVI和QVM对应的源的名称。

0做结束符。

Remarks全局启动AWG使能。

Example例1：使能fovi0的AWG波形。

StsEnableAWG(&fovi0,0);1.2.StsEnableMeas ()StsEnableMeas (FloatingVI，……);ParametersVI填写需要同步测量的VI源名称，此处VI源只能填FPVI、FOVI和QVM对应的源的名称。

0做结束符。

Remarks全局启动AWG同步测量。

Example例1：fovi0和fovi1同步测试。

StsEnableMeas(&fovi0,&fovi1,0);例2：fovi0和fpvi1同步测试。

StsEnableMeas(&fovi0,&fpvi1,0);1.3.StsAWGRun ()StsAWGRun (delayTime);ParametersdelayTimeINT型变量，单位us，设置启动延时。

可以不写，默认为0。

RemarksAWG全局启动。

Example例1：AWG立即输出。

StsAWGRun ();例2：AWG输出开始2ms后继续后面的命令。

StsAWGRun (2000);2.1.FOVI()FOVI(BYTE byChannel)ParameterschannelFOVI逻辑通道号Remarks定义一个FOVI，并指定使用的逻辑通道号。

每个FOVI模块的8个物理通道分别为CH0~3和CH4~7两组，每一个物理通道都可独立进行编程以及选择所绑定的工位。

由于FOVI每四个通道共用一个LOW端，为了确保测量精度，不建议将同一组内的四个物理通道绑到不同的工位上，比如将CH0和CH1绑到site1上，将CH2和CH3绑到site2上是不建议的。

Radiant Gas FireplacesHOME WIFI GUIDEIn-home setup and minimum system requirements.Valor L1, L1 See-Thru, L2, L3, LX1, and LX2 gas fireplaces can be installed or upgraded with a WiFi module, allowing you to connect the fireplace onto the home’s WiFi system and control it using the Valor 10 app on a smart device. Take full control of with intuitive and interactive options—including the ability to program, set a timer, or integrate child safety locks.Introduction To ensure that the installation of your Valor 10 WiFi system goes smoothly, follow these steps to prepare your home before your authorized Valor dealer installs or upgrades your fireplace. Please note the minimum system requirements for optimal performance.Getting Started•Must have an internet connection.• Must have wireless router, located close to the room with the fireplace. Be prepared to add a WiFi repeater if signal strength at the fireplace location is weak.• Have your home WiFi password on hand.• Ensure that your smartphone and or tablet device can run the Valor 10 App. Download to device before installers arrive.• Have 120v AC power available at the fireplace installation location to power V-module.In-Home Setup•Supports Apple iOS 6.0 or later and Android 4.2 and up.•App takes up 60.3 MB for Apple/iOS and 19 MB for Android.•An internet connection is necessary for the app to control the fireplace(s).•SSID Address (home WiFi networ) & password are needed.•Controlling smart device must use home WiFi network (can not be controlled from network outside of the home).• Can control up to 8 gas fireplaces. Each fireplace requires a separate WiFi receiver.Minimum System Requirements For a complete overview of the Valor 10 WiFi system and mobile app, visit /valor10.Search for “Valor 10” or “Valor10 Remote” on the Apple AppStore or Google Play Store todownload the free app. Enjoy!。

4FUNF, UNL, UNY, UNA unions and EL elbowsCl. I, Div. 1 & 2, Groups A, B, C, D A Cl. II, Div. 1, Groups E, F, G Cl. II, Div. 2, Groups F, G Cl. IIIExplosionproof Dust-ignitionproofApplications:UNY and UNF unions are installed in threaded thick wall conduit systems:• UNY – to connect conduit to a conduit fitting, junction box or device enclosure • UNF – to connect conduit to conduit, or to provide a means for future modification of the conduit systemUNA unions are used in conduit and fitting installations when entrance angle is between 90° and 180°.EL elbows are installed in conduit run or in box or fitting hub:• To change direction in threaded rigidconduit run by 90°, or when terminating at a box or fittingFeatures:UNY, UNF and UNL unions have:• Compact design which permits assembly with a minimum of clearance to other adjacent conduit and/or equipment • Strong and durable construction UNA unions:• Have a single clamping nut on angle, making it both a union and a connector • Permit conduit joints at angles between 90° and 180°EL elbows have a smooth interior and are both strong and compact.Standard materials:• UNY, UNF unions – 1/2” to 1” and 11/2”: steel• UNY, UNF unions – 11/4” to 6”: Feraloy iron alloy• UNL, UNA unions – Feraloy iron alloy • EL elbows – Feraloy iron alloy or ductile ironStandard finishes:• Steel – electrogalvanized with chromate treatment• Feraloy iron alloy, malleable iron –electrogalvanized and aluminum acrylic paintUNL 90° angleUNY maleUNF female1/2” - 4”1/2” - 4”5” - 6”5” - 6”Certifications and compliances:NEC/CEC:EL 1/2”, 3/4”, 1”UNF/UNY -105, -215, -205, -305UNL -105, -125, -215, -205• Class I, Divisions 1 & 2, Groups A, B, C, D • Class II, Division 1, Groups E, F, G • Class II, Division 2, Groups F, G • Class IIIUNF/UNY -406, -506, -606, -706, -806, -905, -1005• Class I, Divisions 1 & 2, Groups B, C, D • Class II, Division 1, Groups E, F, G • Class II, Division 2, Groups F, G • Class IIIEL, UNF, UNL, UNY – All Sizes• Class I, Divisions 1 & 2, Groups C, D • Class II, Division 1, Groups E, F, G • Class II, Division 2, Groups F, G • Class III UNA• Class I, Divisions 1 & 2, Group D • Class II, Division 1, Groups E, F, G • Class II, Division 2, Groups F, G • Class III UL standard:• UL1203CSA standard:• C22.2 No. 30A See Certifications and compliances section for classification of each product.B Not UL Listed.C Not available with SA (aluminum) material option.Options:Description Suffix • Copper-free aluminum ..........................SANot available on UNA or 5” and 6” UNY/UNFOrdering information:Hub sizeCat. # UNY Hub sizeCat. # UNF 1/“UNY1051/“UNF105Hub sizeCat. #1/“ to 1/“UNL10544FDimensions (in inches):UNYUNYUNF Hub sizeMaterialLengthMax. dia.LengthMax. dia.1/“Iron25/19/127/19/UNLDim.105125215205a 211/211/27/27/D See Certifications and compliances section for classification of each product.UNY/UNFUNLUNF, UNL, UNY, UNA unions and EL elbowsCl. I, Div. 1 & 2, Groups A, B, C, D D Cl. II, Div. 1, Groups E, F, G Cl. II, Div. 2, Groups F, G Cl. IIIExplosionproof Dust-ignitionproof4FUNAMale(with removable nipple)EL90° male45° female90° female90° male and femaleDimensions (in inches):90° male90° female45° female90° male and female ELE See Certifications and compliances section for classification of each product.F Available in copper-free aluminum. To order, add suffix ‘SA’ to end of catalog number.Ordering information (continued):Male Hub sizeCat. #1/“UNA161”UNA3690° male Hub sizeCat. #1/“EL1951”45° female Hub sizeCat. #1/“EL190° female Hub sizeCat. #1/“F 2/2“90° male and female Hub sizeCat. #1/“F Male Hub sizeLengthWidth1/“45/25/1”5/163/2UNA45° female90° male 90° female 90° male and female Hub sizeaaaa1/“13/17/117/117/ELUNF, UNL, UNY, UNA unions and EL elbowsCl. I, Div. 1 & 2, Groups C, D E Cl. II, Div. 1, Groups E, F, G Cl. II, Div. 2, Groups F, G Cl. IIIExplosionproof Dust-ignitionproof44F UNY and UNF unions –stainless steelCl. I, Div. 1 & 2, Groups A, B, C, DCl. II, Div. 1, Groups E, F, GCl. II, Div. 2, Groups F, GCl. IIIExplosionproofDust-ignitionproofApplications:UNY and UNF unions are installed inthreaded thick wall conduit systems:• UNY – to connect conduit to a conduitfitting, junction box or device enclosure• UNF – to connect conduit to conduit, or toprovide a means for future modification ofthe conduit systemFeatures:UNY and UNF unions have:• Compact design which permits assemblywith a minimum of clearance to otheradjacent conduit and/or equipment• Strong and durable constructionStainless steel benefits:• Self-healing properties help reduce thepenetration of rust/corrosion and eliminatedamage to the fitting• Retain strength in extreme heat andextreme cold conditions• Surface is easy to maintain and keep clean• Easy cleaning capabilities make thesefittings perfect for food processing andother hygienic areas where wash downsare common• Superior strength and durability greatlyreduce replacement – this will lower yourtotal cost of ownership and increase yourreturn on investment• Do not require harsh environment-damaging cleaners to keep them lookinglike newCertifications andcompliances:NEC/CEC:• Class I, Divisions 1 & 2, Groups A, B, C, D• Class II, Division 1, Groups E, F, G• Class II, Division 2, Groups F, G• Class IIIUL standard:• UL1203CSA standard:• C22.2 No. 30Standard material:• 316 stainless steelStandard finish:• PassivatedOrdering information:Hub sizeCat. #UNY Hub sizeCat. #UNF1/“UNY105 SS1/“UNF105 SSDimensions (in inches):UNYHub sizeUNY UNFLength Max. dia.Length Max. dia.1/“215/11/15/11/UNYUNF。

TOP10感受态细胞说明书货号：C1200规格：10×100ul/20×100ul保存：-70℃保存，运输为干冰包装。

自收货之日起液氮保存至少一年，-70℃保存至少6个月。

产品简介：TOP10感受态细胞是采用大肠杆菌TOP10菌株经特殊工艺处理得到的感受态细胞，可用于DNA的化学转化。

使用pUC19质粒检测，转化效率可达108，-70℃保存六个月转化效率不发生改变。

基因型：F_mcrAΔ(mrr-hsd RMS-mcrBC)φ80lacZΔM15△lacⅩ74recA1deoR araD139Δ(ara-leu)7697galU galK rpsL(StrR)endA1nupG特点：一种用于铺制与培养质粒平板和粘粒平板的重级缺陷的抑制型菌株。

其中φ80lacZΔM15基因的产物可与pUC载体编码的β-半乳糖苷酶氨基端实现α-互补，可用于蓝白斑筛选。

该菌株适用于高效的DNA克隆和质粒扩增，能保证高拷贝质粒的稳定遗传。

操作方法：（以下各步骤均为无菌操作）1、将感受态细胞置于冰上融化，以下实验以100ul感受态细胞为例。

2、向感受态细胞悬液中加入需转化的目的DNA，注意目的DNA的体积不要超过感受态细胞悬液体积的十分之一，轻轻旋转离心管以混匀内容物，冰浴放置30分钟。

3、将离心管置于42℃水浴中60-90秒，然后快速转移到冰浴中放置2-3分钟，注意不要摇动离心管。

4、向离心管中加入500ul无菌无抗的SOC或LB培养基，37℃180rpm振荡培养1小时。

目的是使质粒上相关的抗性标记基因表达，使菌体复苏。

5、取适量已转化的感受态细胞涂布含相应抗生素的SOC或LB平板，37℃倒置培养12-16小时。

涂布用量可根据具体实验来调整，如转化的DNA总量较多，可取100ul左右的转化产物涂板；反之，如转化的DNA总量较少，可取200-300ul的转化产物涂板。

过多菌液可以抑制细菌生长。

如果预计的克隆较少，可通过离心后吸除部分培养液，悬浮菌体后将其涂布于一个平板中。