细胞图像处理大作业
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《数字图像处理》期末大作业大作业题目及要求:一、题目:本门课程的考核以作品形式进行。
作品必须用Matlab完成。
并提交相关文档。
二、作品要求:1、用Matlab设计实现图形化界面,调用后台函数完成设计,函数可以调用Matlab工具箱中的函数,也可以自己编写函数。
设计完成后,点击GUI 图形界面上的菜单或者按钮,进行必要的交互式操作后,最终能显示运行结果。
2、要求实现以下功能:每个功能的演示窗口标题必须体现完成该功能的小组成员的学号(后三位)。
1)对于打开的图像可以显示其灰度直方图,实现直方图均衡化和直方图匹配(规定化)。
2)实现灰度图像的对比度增强,要求实现线性变换和非线性变换(包括对数变换和指数变换)。
3)实现图像的缩放变换、旋转变换等。
4)图像加噪(用输入参数控制不同噪声),然后使用空域和频域进行滤波处理。
5)采用robert算子,prewitt算子,sobel算子,拉普拉斯算子对图像进行边缘提取。
6)读入两幅图像,一幅为背景图像,一幅为含有目标的图像,应用所学的知识提取出目标。
3、认真完成期末大作业报告的撰写,对各个算法的原理和实验结果务必进行仔细分析讨论。
报告采用A4纸打印并装订成册。
附录:报告模板《数字图像处理》期末大作业班级:小组编号:组长:小组成员:浙江万里学院计算机与信息学院2013年12月目录(自动生成)1 绘制灰度直方图,实现直方图均衡化和直方图匹配(规定化)1.1 算法原理1.2 算法设计1.3 实验结果及对比分析图1 ××××××图2 ××××××分析***********************************************2 灰度图像的对比度增强2.1 算法原理2.2 算法设计2.3 实验结果及分析3 图形的几何变换3.1 算法原理3.2 算法设计3.3 实验结果及分析4 图像加噪(用输入参数控制不同噪声),然后使用空域和频域进行滤波处理4.1 算法原理4.2 算法设计4.3 实验结果及分析5 采用robert,prewitt,sobel,拉普拉斯算子对图像进行边缘提取5.1 算法原理5.2 算法设计5.3 实验结果及分析6 读入两幅图像,一幅为背景图像,一幅为含有目标的图像,应用所学的知识提取出目标6.1 算法原理6.2 算法设计6.3 实验结果及分析7 小结(感受和体会)(英文版)Two regulations promulgated for implementation is in the party in power for a long time and the rule of law conditions, the implementation of comprehensive strictly strategic plan, implementation in accordance with the rules and discipline to manage the party, strengthen inner-party supervision of major initiatives. The two regulations supporting each other, the < code > adhere to a positive advocate, focusing on morality is of Party members and Party leading cadres can see, enough to get a high standard; < rule > around the party discipline, disciplinary ruler requirements, listed as "negative list, focusing on vertical gauge, draw the party organizations and Party members do not touch the" bottom line ". Here, the main from four square face two party rules of interpretation: the first part introduces two party Revised regulations the necessity and the revision process; the second part is the interpretation of the two fundamental principles of the revision of laws and regulations in the party; the third part introduces two party regulations modified the main changes and needs to grasp several key problems; the fourth part on how to grasp the implementation of the two regulations of the party. < code > and < Regulations > revised the necessity and revised history of the CPC Central Committee the amendment to the Chinese Communist Party members and leading cadres honest politics several guidelines > and < Chinese Communist Party discipline and Punishment Regulations > column 1 by 2015 to strengthenparty laws and regulations focus. Two party regulations revision work lasted a Y ears, pooling the wisdom of the whole party, ideological consensus, draw historical experience, respect for the wisdom of our predecessors, which reflects the unity of inheritance and innovation; follow the correct direction, grasp the limited goals, adhere to the party's leadership, to solve the masses of the people reflect a focus on the problem. The new revision of the < code > and < rule >, reflects the party's 18 and the eighth session of the third, the spirit of the fourth plenary session, reflecting the experience of studying and implementing the General Secretary Xi Jinping series of important speech, reflects the party's eighteen years comprehensive strictly practice. (a) revised two regulations of the party need of < the ICAC guidelines > in < in 1997 Leaders as members of the Communist Party of China clean politics certain criteria (Trial) > based on revised, the promulgation and implementation of January 2010, to strengthen the construction of the contingent of leading cadres play an important role. But with the party to manage the party strictly administering the deepening, has not been able to fully meet the actual needs. Content is too complicated, "eight prohibition, 52 are not allowed to" hard to remember, and also difficult to put into practice; the second is concisely positive advocated by the lack of prohibited provisions excessive, no autonomy requirements; the third is banned terms and discipline law, both with the party discipline, disciplinary regulationsrepeat and Criminal law and other laws and regulations repeat; the fourth is to "clean" the theme is not prominent, not for the existing problems, and is narrow, only needle of county-level leading cadres above. < rule > is in 1997 < Chinese Communist Party disciplinary cases (Trial) > based on revision, in December 2003 the promulgation and implementation, to strengthen the construction of the party play very important role. Along with the development of the situation, which many provisions have been unable to fully meet the comprehensive strictly administering the practice needs. One is Ji law, more than half of the provisions and criminal law and other countries laws and regulations Repetition; two is the political discipline regulations is not prominent, not specific, for violation of the party constitution, damage the authority of Party Constitution of misconduct lack necessary and serious responsibility to pursue; third is the main discipline for the leading cadres, does not cover all Party members. Based on the above situation, need to < the criterion of a clean and honest administration > and < rule > the two is likely to be more relevant regulations first amendment. By revising, really put the authority of Party discipline, the seriousness in the party tree and call up the majority of Party members and cadres of the party constitution of party compasses party consciousness. (II) two party regulations revision process the Central Committee of the Communist Party of China attaches great importance to two regulations revision . Xi Jinping, general books recorded in the FifthPlenary Session of the eighth session of the Central Commission for Discipline Inspection, on the revised regulations < > made clear instructions. According to the central deployment, the Central Commission for Discipline Inspection from 2014 under six months begin study two regulations revision. The Standing Committee of the Central Commission for Discipline Inspection 4 review revised. Comrade Wang Qishan 14 times held a special meeting to study two regulations revision, amendment clarifies the direction, major issues of principle, path and target, respectively held a forum will listen to part of the province (area) secretary of the Party committee, Secretary of the Discipline Inspection Commission, part of the central ministries and state organs DepartmentThe first party committee is mainly responsible for people, views of experts and scholars and grassroots party organizations and Party members. Approved by the Central Committee of the Communist Party of China, on 7 September 2015, the general office of the Central Committee of the Party issued a notice to solicit the provinces (autonomous regions, municipalities) Party, the central ministries and commissions, state ministries and commissions of the Party (party), the General Political Department of the military, every 3 people organization of Party of two regulations revision opinion. Central Commission for Discipline Inspection of extensive solicitation of opinions, careful study, attracting, formed a revised sent reviewers. In October 8 and October 12, Central Committee PoliticalBureau Standing Committee and the Political Bureau of the Central Committee After consideration of the two regulations revised draft. On October 18, the Central Committee of the Communist Party of China formally issued two regulations. Can say, two laws amendment concentrated the wisdom of the whole party, embodies the party. Second, < code > and < Regulations > revision of the basic principles of two party regulations revision work and implement the party's eighteen, ten eight plenary, the spirit of the Fourth Plenary Session of the Eleventh Central Committee and General Secretary Xi Jinping important instructions on the revised < low political criterion > and < Regulations >, highlighting the ruling party characteristics, serious discipline, the discipline quite in front of the law, based on the current, a long-term, advance as a whole, with Bu Xiuding independent < rule > and < rule >. Main principle is: first, adhere to the party constitution to follow. The constitution about discipline and self-discipline required specific, awaken the party constitution of party compasses party consciousness, maintaining the authority of the constitution. General Secretary Xi Jinping pointed out that "no rules, no side round. Party constitution is the fundamental law, the party must follow the general rules. In early 2015 held the eighth session of the Central Commission for Discipline Inspection Fifth Plenary Session of the 16th Central Committee, Xi Jinping again pointed out that constitution is the party must follow the general rules, but also the general rules." the revisionof the < code > and < rule > is Method in adhere to the regulations established for the purpose of combining rule of virtue is to adhere to the party constitution as a fundamental to follow, the constitution authority set up, wake up the party constitution and party rules the sense of discipline, the party constitution about discipline and self-discipline specific requirements. 4 second is to adhere to in accordance with the regulations governing the party and the party. The Party of rule of virtue "de", mainly refers to the party's ideals and beliefs, excellent traditional style. The revised the < code > closely linked to the "self-discipline", insisting on the positive initiative, for all members, highlight the "vital few", emphasized self-discipline, focusing on the morality, and the majority of Party members and the ideological and moral standards. The revised < > Ji method separately, Ji, Ji Y an to Method, as a "negative list", emphasizing the heteronomy, focusing on vertical gauge. Is this one high and one low, a positive reaction, the strict party discipline and practice results transformation for the integration of the whole party to observe moral and discipline requirements, for the majority of Party members and cadres provides benchmarking and ruler. Third, insist on to. In view of the problems existing in the party at the present stage, the main problems of Party members and cadres in the aspect of self-discipline and abide by the discipline to make clearly defined, especially the party's eighteen years strict political discipline and political rules, organization and discipline andto implement the central eight provisions of the spirit against the four winds and other requirements into Disciplinary provisions. Not one pace reachs the designated position, focusing on in line with reality, pragmatic and effective. After the revision of major changes, major changes in the < code > and < rule > modified and needs to grasp several key problems (a) < code > < code > adhere to according to regulations governing the party and party with morals in combination, for at the present stage, the leadership of the party members and cadres and Party members in existing main problems of self-discipline, put forward principles, requirements and specifications, showing Communists noble moral pursuit, reflected at all times and in all over the world ethics from high from low 5 common requirements. One is closely linked to the "self-discipline", removal and no direct relation to the provisions of . the second is adhere to a positive advocate, "eight prohibition" 52 are not allowed to "about the content of the" negative list moved into synchronization amendment < cases >. Three is for all the party members, will apply object from the leadership of the party members and cadres to expand to all Party members, fully embodies the comprehensive strictly required. The fourth is prominent key minority, seize the leadership of the party members and cadres is the key, and put forward higher requirements than the ordinary Party members. Five is to simplify, and strive to achieve concise, easy to understand, easy to remember. The revised < code > is the ruling Party since the first insists ona positive advocate forAll Party members and the self-discipline norms, moral declaration issued to all members of the party and the National People's solemn commitment. > < criterion of a clean and honest administration consists of 4 parts, 18, more than 3600 words. After the revision of the < code >, a total of eight, 281 words, including lead, specification and Party member cadre clean fingered self-discipline norms, etc. Part 3 members low-cost clean and self-discipline, the main contents can be summarized as "four must" "eight code". Lead part, reiterated on ideal and faith, fundamental purpose, the fine traditions and work style, noble sentiments, such as "four must" the principle of requirements, strong tone of self-discipline, The higher request for 6 and supervised tenet, the foothold in permanent Bao the party's advanced nature and purity, to reflect the revised standards requirements. Members of self-discipline norms around the party members how to correctly treat and deal with the "public and private", "cheap and rot" thrifty and extravagance "bitter music", put forward the "four norms". Party leader cadre clean fingered self-discipline norms for the leadership of the party members and cadres of the "vital few", around the "clean politics", from civil servant of the color, the exercise of power, moral integrity, a good family tradition and other aspects of the leadership of the party members and cadres of the "four norms" < > < norm norm. "The Party member's self-discipline norms" and "party members and leading cadre clean fingered self-discipline norms," atotal of eight, collectively referred to as the "eight". "Four must" and "eight" of the content from the party constitution and Party's several generation of leaders, especially Xi Jinping, general secretary of the important discussion, refer to the "three discipline and eight points for attention" statements, and reference some embody the Chinese nation excellent traditional culture essence of epigrams. (2) the revised regulations, the main changes in the revised Regulations > to fully adapt to the strictly requirements, reflects the according to the regulations governing the law of recognition of deepening, the realization of the discipline construction and Jin Ju. < rule > is party a ruler, members of the basic line and follow. And the majority of Party members and cadres of Party organizations at all levels should adhere to the bottom line of thinking, fear discipline, hold the bottom line, as a preventive measure, to keep the party's advanced nature and purity. 1, respect for the constitution, refinement and discipline. Revised < rule > from comprehensive comb physical constitution began, the party constitution and other regulations of the Party of Party organizations and Party discipline requirements refinement, clearly defined in violation of the party constitution will be in accordance with regulations to give the corresponding disciplinary action. The original 10 categories of misconduct, integration specification for political discipline, discipline, honesty and discipline masses Ji Law and discipline and discipline and other six categories, the content of < rule >real return to Party discipline, for the majority of Party members and listed a "negative list. 7 2, highlighting the political discipline and political rules. > < Regulations according to the stage of the discipline of outstanding performance, emphasizing political discipline and political rules, organization and discipline, in opposition to the party's leadership and the party's basic theory, basic line, basic program and basic experience, the basic requirement of behavior made prescribed punishment, increase the cliques, against the organization such as violation of the provisions, to ensure that the central government decrees and the Party of centralized and unified. 3, adhere to strict discipline in the law and discipline In front, Ji separated. Revised < Regulations > adhere to the problem oriented, do Ji separated. Any national law existing content, will not repeat the provisions, the total removal of 79 and criminal law, repeat the content of the public security management punishment law, and other laws and regulations. In the general reiterated that party organizations and Party members must conscientiously accept the party's discipline, die van comply with national laws and regulations; at the same time, to investigate violations of Party members and even criminal behavior of Party discipline and responsibility, > < Regulations distinguish five different conditions, with special provisions were made provisions, so as to realize the connection of Party discipline and state law. 4, reflect Wind building and anti-corruption struggle of the latest achievements. < rule > the party's eighteen yearsimplement the spirit of the central provisions of the eight, against the requirements of the "four winds" and transformation for disciplinary provisions, reflecting the style construction is always on the road, not a gust of wind. In the fight against corruption out of new problems, increase the trading rights, the use of authority relatives profit and other disciplinary terms. Prominent discipline of the masses, the new against the interests of the masses and ignore the demands of the masses and other disciplinary terms and make provisions of the disposition and the destruction of the party's close ties with the masses.Discipline to protect the party's purpose. 8 of these regulations, a total of three series, Chapter 15, 178, more than 24000 words, after the revision of the regulations a total of 3 series, Chapter 11, 133, 17000 words, divided into "general" and "special provisions" and "Supplementary Provisions" Part 3. Among them, add, delete, modify the provisions of the proportion of up to nearly 90%. 1, the general general is divided into five chapters. The first chapter to the regulations of the guiding ideology, principles and scope of application of the provisions, highlight the strengthening of the party constitution consciousness, maintenance the authority of Party Constitution, increase the party organizations and Party members must abide by the party constitution, Y an Centralized centralized, would examine at all levels of the amended provisions implementing and maintaining Party discipline, and consciously accept the party discipline,exemplary compliance with national laws and regulations. The second chapter of discipline concept, disciplinary action types and effects of the regulations, will be a serious warning from the original a year for a year and a half; increase the Party Congress representative, by leaving the party above (including leave probation) punishment, the party organization should be terminated its representative qualification provisions. The third chapter of the disciplinary rules of use prescribed in the discipline rectifying process, non convergence, not close hand classified as severely or heavier punishment. "Discipline straighten "At least eighteen years of five years, these five years is to pay close attention to the provisions of the central eight implementation and anti -" four winds ". The fourth chapter on suspicion of illegal party disciplinary distinguish five different conditions, with special provisions were made provisions, to achieve effective convergence of Party and country 9 method. < rule > the provisions of Article 27, Party organizations in the disciplinary review found that party members have committed embezzlement, bribery, dereliction of duty dereliction of duty and other criminal law act is suspected of committing a crime shall give cancel party posts, probation or expelled from the party. The second is < Regulations > Article 28 the provisions of Party organizations in the disciplinary review But found that party members are stipulated in the criminal law, although not involved in a crime shall be investigated for Party discipline and responsibility shouldbe depending on the specific circumstances shall be given a warning until expelled punishment. This situation and a difference is that the former regulation behavior has been suspected of a crime, the feeling is quite strict, and the latter for the behavior not involving crime, only the objective performance of the provisions of the criminal code of behavior, but the plot is a crime to slightly. < Regulations > the 29 provisions, Party organizations in the discipline review found that party members and other illegal behavior, affect the party's image, the damage to the party, the state and the people's interests, we should depend on the situation Seriousness given disciplinary action. The loss of Party members, seriously damaging the party's image of behavior, should be given expelled from the party. At this article is party member is in violation of the criminal law outside the other illegal acts, such as violates the public security administration punishment law, customs law, financial laws and regulations behavior. The fourth is < cases > Article 32 stipulates, minor party members and the circumstances of the crime, the people's Procuratorate shall make a decision not to initiate a prosecution, or the people's court shall make a conviction and exempted from criminal punishment shall be given within the party is removed from his post, probation or expelled from the party. Party members and crime, sheets were fined in accordance with For acts; the principal Ordinance amended the provisions of the preceding paragraph. This is the new content, in order to achieve Ji method effectiveconvergence. Five is < > the thirty third article 10 of the provisions, the Party member due to an intentional crime is sentenced to criminal law (including probation) sheets or additional deprivation of political rights; due to negligence crime and was sentenced to three years or more (excluding three years) a penalty, shall give expelled punishment. Due to negligence crime is convicted and sentenced to three years (including three years) in prison or be sentenced to public surveillance, detention, shall in general be expelled from the party. For the individual may not be expelled from the party, should control Approval. This is followed and retained the original > < Regulations the provisions of punishment party authorization rules and report to a level party organizations. For is "party members with criminal acts, and by the criminal punishment, generally should be expelled from the party". The fifth chapter of probationary Party member of the discipline and discipline after missing members of the treatment and punishment decisions, such as the implementation of the provisions, clear the related party discipline and punishment decision made after, for duties, wages and other relevant alteration formalities for the longest time. 2, sub sub section will the original regulations of10 categories of acts of violation of discipline integration revised into 6 categories, respectively, in violation of the punishments for acts of political discipline "in violation of discipline behavior of punishment" in violation of integrity of disciplinary action points "of violation punishments for actsof mass discipline" "the violation of work discipline, punishment" in violation of discipline of life behavior punishment "6 chapters. 3, annex" Supplementary Provisions "clear authority making supplementary provisions of, cases of interpretative organ, as well as regulations implementation time and retroactivity etc.. 11 (3) learning understanding > < regulations needs to grasp several key problems The first problem -- about the violation of political discipline behavior > < new ordinance chapter 6 the political discipline column for the six disciplines, that is the main opposition to Party leadership and the opposition of the basic theory, basic line, basic program and basic experience, basic requirements of misconduct made provisions of the disposition, especially the eighteen since the CPC Central Committee put forward the Yan Mingzheng treatment of discipline and political rules requirements and practical achievements transformation for Discipline article, increase the false debate central policies, cliques, against the organization review, make no discipline of the principle of harmony terms. These are the party's eighteen years in comprehensive strictly Process combined with the practice of rich content. (1) false debate the central policies and undermine the Party of centralized and unified the problem is made in accordance with the provisions of the party constitution. Constitution in general programme requirements adhere to democratic centralism is one of the requirements of the construction of the party must adhere to the four cardinal. Applicationof this principle is not only the party the basic organization principle and is also the mass line in party life, it requires that we must fully develop inner-party democracy, respect for the dominant position of Party members, safeguarding the Party member democratic rights, give full play to the enthusiasm and creativity of the party organizations at all levels and Party members, at the same time, also must implement the right concentration, ensure the party's mission < the chaos in unity and concerted action to ensure that the party's decision to get quickly and effectively implementing. The Party Central Committee formulated the major principles and policies, through different channels and ways, fully listen to the party organizations and Party members of the opinions and suggestions, but 12 is some people face to face not to say back blather "" will not say, after the meeting said, "" Taiwan does not say, and nonsense ", in fact, not only disrupt the people thought, some causing serious consequences, the damage to the Party of the centralized and unified, hinder the central policy implementation, but also a serious violation of the democratic system of principles. There is no doubt that shall, in accordance with the Regulations > 4 Specified in Article 6 to give the appropriate punishment. For did not cause serious consequences, to give criticism and education or the corresponding tissue processing. (2) about the destruction of the party's unity < New Regulations > the forty eighth to fifty second article, to damage Party's unity unified and violation of political discipline, punishment situationmade explicit provisions. Article 52 of the new "in the party get round group, gangs seek private gain, cliques, cultivate private forces or through the exchange of interests, for their own to create momentum and other activities to gain political capital, given a serious warning or withdraw from their party posts disposition; if the circumstances are serious, to give Leave a party to observation or expelled from the party. (3) on against the organization review of the provisions of the constitution, party loyalty honesty is party members must comply with the obligations. Members must obey the organization decision, shall not violate the organization decided encounters by asking questions to find organization, rely on the organization, shall not deceive the organization, against the organization. For example, after the investigation does not take the initiative to explain the situation, but to engage in offensive and defensive alliance, hiding the stolen money is against survey organization, is a violation of the behavior of political discipline. Article 24 of the original > < Regulations, although the provisions of the interference, hinder group review the behavior of the fabric can be severely or 13 Aggravated punishment, but did not put this kind of behavior alone as a discipline for qualitative amount of discipline. > < new regulations increase the Article 57, "anti organization review, one of the following acts, given a warning or serious warning; if the circumstances are relatively serious, giving removed from or placed on probation within the party post; if the circumstances are serious, give。
1、下图是一用于干涉原理进行测试的干涉场图像,要求判读条纹的间距,请给出图像处理的方案并说明每一步的作用及其对其它处理步骤可能产生的影响。
解:步骤与思路:○1.进行模糊处理,消除噪声○2.边缘检测,进行图像增强处理○3.二值化图像,再进行边缘检测,能够得到很清晰的边界。
○4.采用横向标号法,根据值为1像素在标号中的相邻位置可以确定间距I=imread('xz mjt.bmp');I1=medfilt2(I); %对图像中值滤波imshow(I1);[m,n]=size(I1);for i=1:mfor j=1:nif(I1(i,j)<100) %阈值为100I1(i,j)=255;elseI1(i,j)=0; %进行二值化endendendfigure;imshow(I1);Y1=zeros(1,25);y2=y1;c=y2;i=100;for j=1:1200if (I1(i,j)==255&&I1(i,j+1)==0)Y1=j+1;endif (I1(i,j)==0&&I1(i,j+1)==255)Y2=j;endendfor i=1:25c=Y2(i)-Y1(i)endc %找出每两个条纹之间的距离2. 现有8个待编码的符号m0,……,m7,它们的概率分别为0.11,0.02,0.08,0.04,0.39,0.05,0.06,0.25,利用哈夫曼编码求出这一组符号的编码并画出哈夫曼树。
3. 请以图像分割方法为主题,结合具体处理实例,采用期刊论文格式,撰写一篇小论文。
各种算子对图像进行边缘检测效果的研究图像分割是根据需要将图像划分为有意义的若干区域或部分的图像处理技术。
通过边缘检测在Matlab 中实现方法,及用四叉数分解函数进行区域分割的方法,掌握了Matlab 区域操作函数的使用和图像分析和理解的基本方法,并学到了'roberts','sobel','prewitt','canny','log'算子对图像进行边缘检测的不同效果。
医学影像分析中的细胞图像处理技术研究随着医学技术的不断发展,医学影像学已经成为了现代医学中不可或缺的一部分。
医学影像学通过对人体进行影像的获取、处理和诊断,对疾病的早期发现和治疗提供了重要的依据。
其中,细胞图像处理技术在医学影像学中发挥着重要作用。
本文将从细胞图像获取、处理和分析三个方面对医学影像分析中的细胞图像处理技术进行探讨。
一、细胞图像获取细胞图像获取是医学影像分析中的第一步,其质量将直接影响到后续的分析和诊断结果。
目前细胞图像获取的主要技术有多光子显微镜、荧光共聚焦显微镜和电子显微镜等。
多光子显微镜可以通过非线性光学效应在样品中产生荧光,对活体细胞进行成像,不需要用到染色剂。
但是,多光子显微镜成像的分辨率相对其他技术较低,不能获得非常高精度的细胞图像。
荧光共聚焦显微镜可以通过三维成像技术获得三维细胞图像,其分辨率比多光子显微镜高。
但是,荧光共聚焦成像需要样品中具有荧光素,不能获得非荧光样品的图像。
电子显微镜是一种非常高分辨率的成像技术,可以对细胞进行超高分辨率成像。
但是,电子显微镜成像需要细胞样品的处理、培养和金属蒸镀等操作,不适用于活体细胞成像。
另外,电子显微镜成像的价格也比较高,不适合大规模使用。
综合以上几种技术,目前通过荧光共聚焦显微镜获得的细胞图像应用相对广泛。
荧光共聚焦显微镜成像技术可以通过样品标记荧光素,不仅可以获得三维高分辨率的图像,而且可以在细胞内部无破坏地观察生化反应的过程。
二、细胞图像处理细胞图像获取获得的是原始的图像数据,需要经过一系列的处理和拼接才能应用到疾病诊断中。
目前细胞图像处理的主要应用领域包括了细胞形态分析、信号分析和功能分析等。
细胞形态分析是细胞图像处理的基础,其主要内容包括细胞的形状、大小和数量等指标的测量。
这些指标可以反映出细胞的生长状态、病变程度和受到药物干预的程度等信息。
细胞形态分析的方法包括图像分割、特征提取和分类等技术。
信号分析是指对细胞内分子和物质在空间和时间上的定量分析。
(完整word版)数字图像处理实验报告6数字图像处理与分析实验报告学院:班级:姓名:学号:实验六细胞图像的分割与测量一、实验目的1. 通过分析细胞图像特点,完成细胞图像的分割和测量,并分析测量结果。
2. 将图像预处理、分割、分析等关键技术结合起来,理论与实践相结合,提高图像处理关键技术的综合应用能力。
二、实验要求1. 对比中值、均值和形态学开闭运算对细胞图像的滤波效果,选择适用于细胞图像的滤波方法2. 运用大津阈值对细胞图像分割,观察分割后噪声情况,观察目标边缘处的分割效果;(使用函数:im2bw)3. 实现连通区域的编号;(使用函数:bwlabel)4. 计算各连通区域的相关信息,如面积、重心等。
(使用函数:regionprops )三、实验步骤1、预处理去噪x=imread('C:\Documents and Settings\Administrator\桌面\CHEN2-7.BMP');y=medfilt2(rgb2gray(h));subplot(2,2,1)imshow(x)title('原图像');subplot(2,2,2)imshow(y)title('中值滤波处理');I=fspecial('average')z=imfilter(rgb2gray(x),I);subplot(2,3,4)imshow(z)title('均值滤波处理');se = strel('ball',5,5);m = imopen(rgb2gray(x),se);subplot(2,3,5)imshow(m)title('形态学开运算处理');se = strel('ball',5,5);n = imclose(rgb2gray(x),se);subplot(2,3,6)imshow(n)title('形态学闭运算处理');2、大津阈值分割先做出灰度图像的直方图,根据直方图选取合适的分割灰度值x=imread('C:\Documents and Settings\Administrator\桌面\CHEN2-7.BMP');b=rgb2gray(x);imhist(b);title('灰度直方图');由上图可知,选择阈值在185/255附近可以达到最好的分割效果,则有:x=imread('C:\Documents and Settings\Administrator\桌面\CHEN2-7.BMP'); b=rgb2gray(x);y1=medfilt2(b);w1=im2bw(y1,185/255);h=fspecial('average') ;y2=imfilter(b,h);w2=im2bw(y2, 185/255);se=strel('line',11,90);y3=imopen(b,se);w3=im2bw(y3, 185/255);y4=imclose(b,se);w4=im2bw(y4, 185/255);figuresubplot(2,2,1)imshow(w1)title('中值大津阈值分割');subplot(2,2,2)imshow(w2)title('均值大津阈值分割');subplot(2,2,3)imshow(w3);title('开运算大津阈值分割');subplot(2,2,4)imshow(w4);title('闭运算大津阈值分割');3、目标编号标记x=imread('C:\Documents and Settings\Administrator\桌面\CHEN2-7.BMP'); b=rgb2gray(x);se=strel('ball',5,5);y4=imclose(b,se);w4=im2bw(y4, 185/255);z=imcomplement(w4);subplot(2,2,1)imshow(z);title('取反后图像')z=bwareaopen(z,200);subplot(2,2,2);imshow(z);title('去除像素点小于200的部分')BW = logical(z);L = bwlabel(BW,4);subplot(2,2,3);imshow(L);title('四连通')M=bwlabel(BW,8);Subplot(2,2,4)imshow(M);title('八连通')4、测量各个细胞的面积等参数x=imread('C:\Documents and Settings\Administrator\桌面\CHEN2-7.BMP'); b=rgb2gray(x);se = strel('ball',5,5);I0=imclose(b,se);I11=im2bw(I0,185/256);I12=imcomplement(I11);I13= bwlabel(I12);s = regionprops(I13, 'centroid');centroids = cat(1, s.Centroid);figure(1);imshow(I13)title('重心标记图');hold onplot(centroids(:,1), centroids(:,2), 'r*')hold offm=regionprops(I13,'Area');areas=cat(1, m.Area);figure(3);plot(areas)如图为重心标记和各区域面积分布图:1.为何需要预处理?直接分割的效果如何?答:如果不进行预处理,在后续处理时如进行阈值分割会产生边缘毛刺,使效果不明显;2.选择何种预处理方法?a) 中值适合于去除脉冲噪声和图像扫描噪声,同时不会使图像变模糊,但对消除细节较多的图像不适合用中值滤波;b) 均值可以有效的是噪声得到消除,但同时图像变得模糊,丢失了一些图像的细节部分;c) 形态学开运算对于消除背景噪声有很好的效果,尤其是一些胡椒噪声;d) 形态学闭运算对消除前景噪声效果较好,如:沙眼噪声;通过以上分析及结合图像处理效果可以得出,利用形态学开闭运算对滤除图像中的沙眼噪声和胡椒噪声效果较好;3.分析预处理的目的,有针对性的选择合适的方法答:预处理的目的是为了事先消除图像的噪声,好为后处理做准备;四、思考题1.若将预处理去噪的步骤省掉,则如何在目标编号的过程中加入滤波处理;答:若预处理中没有去噪步骤,从图像处理结果可以看出,经过阈值分割后,图像中还有很多椒盐噪声,要在编号中滤除这些噪声,可通过形态学开运算后,再进行取反操作等后续操作;2.将去噪过程与阈值分割前后调换,选择哪种滤波方法可以滤除二值图像上的噪声;答:通过阈值分割之后,图像中有很多胡椒噪声,可通过形态学开操作将其去除;3.总结大津阈值在细胞图像分割中存在的问题,想一想你所学的算法中哪种算法更适合于细胞图像的分割。
细胞图像处理方法的使用方法与细胞计数准确性评估细胞图像处理方法是一种应用于生物医学研究中的重要技术,它能够通过数字图像处理和计算机视觉算法对细胞图像进行分析和处理,实现细胞计数、形态特征提取等功能。
本文将介绍细胞图像处理方法的使用方法以及如何评估细胞计数准确性。
一、细胞图像处理方法的使用方法1. 图像获取:首先,需要通过显微镜或者其他成像设备获取细胞图像。
图像获取过程中应注意调整曝光时间、聚焦程度等参数,以获得清晰、准确的细胞图像。
2. 图像预处理:对于原始细胞图像,通常需要进行预处理,以去除噪声、增强图像对比度等。
预处理步骤可包括灰度处理、平滑滤波、边缘检测等。
3. 分割与提取:细胞图像通常需要进行细胞分割,将细胞与背景区分开来。
分割方法包括阈值分割、基于边缘的分割、基于区域的分割等。
分割完成后,可以使用形态学操作等方法进一步提取细胞的形态特征。
4. 特征提取与量化:通过细胞图像处理方法,可以提取细胞的特征,如面积、周长、形状等。
这些特征可以反映细胞的状态和功能。
特征提取可以使用传统的几何特征提取方法,也可以使用机器学习方法,如支持向量机、神经网络等。
5. 细胞计数:细胞计数是细胞图像处理的一个重要应用领域。
细胞计数可以通过手工标注或自动计数的方式进行。
手工标注通常需要在图像上逐个标记细胞,然后统计数量。
自动计数是一种常见的方法,通过图像处理技术实现自动定位和计数,提高效率和准确性。
二、细胞计数准确性评估在使用细胞图像处理方法进行细胞计数时,准确性评估是必不可少的步骤。
以下是一些常用的评估方法:1. 标准比较:与手工标注结果进行比较是一种常见的评估方法。
将细胞图像处理得到的计数结果与手工标注结果进行对比,计算误差率。
误差率越小,则表示细胞计数准确性越高。
2. 重复实验:进行多次实验,并统计不同实验的计数结果之间的一致性。
如果多次实验得到的计数结果相近,则说明细胞计数具有较高的准确性。
3. 计数对比:将细胞图像处理方法得到的计数结果与其他计数方法进行对比。
数字图像处理1.图像工程的三个层次是指哪三个层次?各个层次对应的输入、输出对象分别是什么?①图像处理特点:输入是图像,输出也是图像,即图像之间进行的变换。
②图像分割特点:输入是图像,输出是数据。
③图像识别特点:以客观世界为中心,借助知识、经验等来把握整个客观世界。
“输入是数据,输出是理解。
2.常用的颜色模型有哪些(列举三种以上)?并分别说明颜色模型各分量代表的意义。
①RGB(红、绿、蓝)模型②CMY(青、品红、黄)模型③HSI(色调、饱和度、亮度)模型3.什么是图像的采样?什么是图像的量化?1.采样采样的实质就是要用多少点来描述一幅图像,采样结果质量的高低就是用前面所说的图像分辨率来衡量。
简单来讲,对二维空间上连续的图像在水平和垂直方向上等间距地分割成矩形网状结构,所形成的微小方格称为像素点。
一副图像就被采样成有限个像素点构成的集合。
例如:一副640*480分辨率的图像,表示这幅图像是由640*480=307200个像素点组成。
2.量化量化是指要使用多大范围的数值来表示图像采样之后的每一个点。
量化的结果是图像能够容纳的颜色总数,它反映了采样的质量。
针对数字图像而言:采样决定了图像的空间分辨率,换句话说,空间分辨率是图像中可分辨的最小细节。
量化决定了图像的灰度级,即指在灰度级别中可分辨的最小变化。
数字图像处理(第三次课)调用图像格式转换函数实现彩色图像、灰度图像、二值图像、索引图像之间的转换。
图像的类型转换:对于索引图像进行滤波时,必须把它转换为RGB图像,否则对图像的下标进行滤波,得到的结果是毫无意义的;2.用MATLAB完成灰度图像直方图统计代码设计。
6789101112131415161718192021222324252627282930title('lady-lenna');if isrgb(a);b=rgb2gray(a);%RGB转换为灰度图像endsubplot(2,2,2);imshow(b);%显示图像title('ladygaga-lenna');[m,n]=size(a);%返回图像大小e=zeros(1,256);for k=0:255for i=1:mfor j=1:nif a(i,j)==ke(k+1)=e(k+1)+1;%灰度值相同的进行累加endendendendsubplot(2,2,4);bar(e);%画图像的灰度直方图title('灰度直方图');c=imrotate(a,20);%图像的旋转subplot(2,2,3);imshow(c);数字图像处理(第四次课)编写matlab函数,实现在医学图像中数字减影血管造影。
大作业指导书题目:数字图像处理院(系):物联网工程学院专业: 计算机班级:计算机1401-1406指导老师:学号:姓名:设计时间: 2016-2017学年 1学期摘要 (3)一、简介 (3)二、斑点数据模型.参数估计与解释 (4)三、水平集框架 (5)1.能量泛函映射 (5)2.水平集传播模型 (6)3.随机评估方法 (7)四、实验结果 (8)五、总结 (11)基于水平集方法和G0模型的SAR图像分割Abstract(摘要)这篇文章提出了一种分割SAR图像的方法,探索利用SAR数据中的统计特性将图像分区域。
我们假设为SAR图像分割分配参数,并与水平集模型相结合。
分布属于G分布中的一种,处于数据建模的目的,它们已经成功的被用于振幅SAR图像中不同区域的建模。
这种统计数据模型是驱动能量泛函执行区域映射的基础,被引用到水平集传播数值方案中,将SAR 图像分为均匀、异构和极其异构区域。
此外,我们引入了一个基于随机距离和模型的评估过程,用于量化我们方法的鲁棒性和准确性。
实验结果表明,我们的算法对合成和真实SAR 数据都具有准确性。
+简介1、Induction(简介)合成孔径雷达系统是一种成像装置,采用相干照明比如激光和超声波,并会受到斑点噪声的影响。
在SAR图像处理过程中,返回的是斑点噪声和雷达切面建模在一起的结果。
这个积性模型(文献[1])因包含大量的真实SAR数据,并且在获取过程中斑点噪声被建模为固有的一部分而被广泛应用。
因此,SAR图像应用区域边界和目标检测变得更加困难,可能需要斑点去除。
因此,斑点去除是必需的,有效的方法可以在文献[2][3][4][5][6][7][8][9][10]中找到。
对于SAR图像分割,水平集方法构成一类基于哈密顿-雅克比公式的重要算法。
水平集方法允许有效的分割标准公式,从文献[12]中讨论的传播函数项可以得到。
经典方法有着昂贵的计算成本,但现在的水平集的实现配置了有趣的低成本的替换。
生物学研究中的细胞图像分析技术随着科技的不断发展,生物学研究领域中出现了越来越多的图像分析技术。
细胞图像分析技术便是其中之一,它研究的对象是生物细胞,可以通过图像分析技术对细胞结构、形态、功能等进行定量分析,从而深入研究生物现象。
一. 细胞图像处理技术细胞图像处理技术是细胞图像分析技术的重要组成部分,其主要功能是对细胞图像进行清晰化、分割、分析等操作。
图像处理的第一步是对细胞图像进行灰度化或颜色标定,将图像数字化并导入到计算机系统中。
然后通过滤波、边缘检测、形态学处理等方法对图像进行清晰化和分割。
最后,对分割后的细胞图像进行特征提取和分析,研究其形态、大小、密度等参数,了解细胞的生长发育和变异规律。
二. 细胞图像分析技术在细胞病理学中的应用细胞图像分析技术可以应用于细胞病理学中的细胞样本分析、肿瘤标记物检测、细胞核分析等。
在细胞样本分析中,可以通过图像处理技术对样本中的不同类型细胞进行分离识别,了解其分布、数量等信息。
在肿瘤标记物检测中,可以根据细胞核与细胞周边区域的对比度等特征,进行精准的细胞核图像分割和特征提取,从而达到肿瘤标记物检测的目的。
在细胞核分析中,对细胞核的形态、大小、颜色、纹理等特征进行分析可以有效地帮助判断疾病及其程度。
因此,细胞图像分析技术在细胞病理学中的应用为疾病的早期诊断和治疗提供了可靠的技术手段。
三. 细胞图像分析技术在细胞生物学中的应用细胞图像分析技术还可以应用于细胞生物学中,特别是在微观细胞研究方面。
通过图像分析技术,可以对细胞内蛋白质、RNA、DNA等物质进行精细的定量研究,进一步了解其分布、运动、交互等特征。
在细胞动力学方面,可以通过对细胞形态和运动轨迹的分析,研究细胞的生长发育和代谢过程。
在细胞分子生物学方面,可以通过对细胞核、染色体、内质网等亚细胞结构的形态学和功能特征的研究,揭示生命活动的本质规律。
总之,细胞图像分析技术在生物学研究中发挥了重要的作用,为研究生物现象提供了新的思路和手段。
数字图像处理期末大作业一、问题描述实现第十章中采用Hough变换来检测图像中圆的过程。
,通过包括平滑(把细节去除),边缘检测(得到轮廓)以及Hough变换得到的圆,并把结果叠加到原来的灰度图像上。
给出具体的过程,中间结果,最后结果,实现的代码,并写出报告。
二、图片的获取以及预处理针对老师提供的一副硬币图片,要求检测出其中的hough圆,并叠加到原图像上以便增强图像。
在检测hough圆之前,首先要对图像进行平滑处理,进行拉普拉斯变换,然后检测垂直方向,水平方向,+45度和-45度方向的边缘,将四个方向的边缘叠加起来,得到总的边缘,对该图像进行二值化,然后对得到的图像检测其hough圆,得到圆形边缘,将该图像叠加到原图像上,就实现了图像边缘增强的目的。
三、图像处理算法的基本原理以及处理结果本实验流程图如下:1.读取图像图像处理的第一步就是对所采集的图像进行读入,本次实验的输入图像是一幅灰度图像,不需要将图像转换成为灰度图像,直接利用函数imread ()完成。
原图像如下所示:原图像2.图像预处理在图像预处理中,我们完成了两步工作,首先使用方差为1的高斯噪声对图像进行平滑,然后进行拉普拉斯变换,即)],(*)([2y x f r h ∇,222r 2e 21)(σσ-=πr h 为方差为2σ的高斯噪声,本实验中12=σ。
又),(*)]([)],(*)([22y x f r h y x f r h ∇=∇,其中2224222]2[)(σσσr er r h --=∇,将)(2r h ∇和),(y x f 分别进行傅里叶别换,将其逐点相乘,再进行傅里叶反变换,就得到了预处理后的图像。
3.边缘检测对水平,垂直,+45度,-45度方向进行边缘检测,本实验中我们采用了Prewitt 梯度算子,它用于检测水平方向,垂直方向,+45度方向和-45度方向的掩膜分别如下:水平掩膜 垂直掩膜 +45度掩膜 -45度掩膜使用这四个掩膜分别对上一步得到的图像逐点进行处理,就可以得到四个方向的边缘了(本实验中边缘的一个像素都不处理),再将它们加起来,就得到了总的边缘,实验结果如下:水平边缘垂直边缘-45度边缘总的边缘如下图所示:4.二值化对上图得到的图像进行二值化,这里我采用的是循环方式确定图像全局阈值,即首先以图像的平均值作为阈值,将图像分成两部分,分别求两部分的平均值,新的阈值为这两个平均值的均值,重复上述过程,直到两次阈值之差小于特定的值时停止,并以最后一次得到的阈值对图像进行二值化,本实验中我要求两次阈值之差小于0.5时停止,最后得到的全局阈值为 -102.1332,二值化后的图像如下所示:二值化后的图像5.Hough变换检测圆形边界Hough 变换的原理就是利用图像全局特征将边缘像素连接起来组成区域封闭边界,它将图像空间转换到参数空间,在参数空间对点进行描述,达到检测图像边缘的目的。
(一)灰度灰度(grey level)指图像各种分颜色的深浅程度。
比较高级的图像分析仪可将灰度分成为256级,也有的只能将灰度分成64级,总之都是2n,24是26256是28。
免疫细胞化学标本上反应产物的染色深浅即可用灰度来表示。
能将一张标本上不同染色深度区分为几十或更多的等级,这是人眼所不及的。
因此,某些实验的结果,如果用光学显微镜作一般的观察,似乎实验组与对照组无明显差异,而用图像灰度法经统计学分析,则可反映出显著性差异,说明仅仅用显微镜观察是不够的,在某些情况下可能会辜负了制片过程中所花费的大量精力,而得不到应有的结果。
由于免疫反应产物在细胞内不一定是均匀分布的,在同一个细胞中就可产生各种灰度,这种情况如何进行比较?有两类方法可供选用:一种是测每个细胞的平均灰度,近年较新型的图像分析系统有此性能,而比较早期的或比较简单的图像分析系统就没有这种性能,例如某一细胞在电视屏幕上占有120个像素,按灰度级进行测量后,也许120个像素中有十多种不同的灰度,分析仪可立即显示其平均灰度。
另一种方法是在反应产物不均匀的细胞中可用每一种灰度所占的百分率来表示,例如你要观察1000个细胞,在这些细胞中各种灰度所占的百分率可测出,并作出曲线,或用其他统计学方法处理,来进行比较。
如果用彩色图像分析仪,除了上述的灰度测量以外,还可以将不同的灰度编成不同的颜色,使图像更鲜明美丽,而且便于测量。
彩色有两种情况:在屏幕上出现与标本一样的颜色称为真色(real color),如果将不同的灰度转换成不同的颜色则称为假色(pseudo color),应用假色的目的是增强对比度,来改善对细胞结构的视觉识别,同时可增强轮廓用来强调结构的细节。
例如,免疫细胞化学中常用的非标记抗体过氧化物酶—抗过氧化物酶法,最终反应产物呈棕色,假设在脑内某核团的许多细胞中,呈现出不同深度的棕色,深浅差别较大者显示在屏幕上人眼还能区别,若差别小的则不易区别了,操作者可将灰度11~15用红色显示,灰度16~20用蓝色显示、灰度21~25用黄色显示、灰度26~30用绿色显示等。
(一)灰度灰度(grey level)指图像各种分颜色的深浅程度。
比较高级的图像分析仪可将灰度分成为256级,也有的只能将灰度分成64级,总之都是2n,24是26256是28。
免疫细胞化学标本上反应产物的染色深浅即可用灰度来表示。
能将一张标本上不同染色深度区分为几十或更多的等级,这是人眼所不及的。
因此,某些实验的结果,如果用光学显微镜作一般的观察,似乎实验组与对照组无明显差异,而用图像灰度法经统计学分析,则可反映出显著性差异,说明仅仅用显微镜观察是不够的,在某些情况下可能会辜负了制片过程中所花费的大量精力,而得不到应有的结果。
由于免疫反应产物在细胞内不一定是均匀分布的,在同一个细胞中就可产生各种灰度,这种情况如何进行比较?有两类方法可供选用:一种是测每个细胞的平均灰度,近年较新型的图像分析系统有此性能,而比较早期的或比较简单的图像分析系统就没有这种性能,例如某一细胞在电视屏幕上占有120个像素,按灰度级进行测量后,也许120个像素中有十多种不同的灰度,分析仪可立即显示其平均灰度。
另一种方法是在反应产物不均匀的细胞中可用每一种灰度所占的百分率来表示,例如你要观察1000个细胞,在这些细胞中各种灰度所占的百分率可测出,并作出曲线,或用其他统计学方法处理,来进行比较。
如果用彩色图像分析仪,除了上述的灰度测量以外,还可以将不同的灰度编成不同的颜色,使图像更鲜明美丽,而且便于测量。
彩色有两种情况:在屏幕上出现与标本一样的颜色称为真色(real color),如果将不同的灰度转换成不同的颜色则称为假色(pseudo color),应用假色的目的是增强对比度,来改善对细胞结构的视觉识别,同时可增强轮廓用来强调结构的细节。
例如,免疫细胞化学中常用的非标记抗体过氧化物酶—抗过氧化物酶法,最终反应产物呈棕色,假设在脑内某核团的许多细胞中,呈现出不同深度的棕色,深浅差别较大者显示在屏幕上人眼还能区别,若差别小的则不易区别了,操作者可将灰度11~15用红色显示,灰度16~20用蓝色显示、灰度21~25用黄色显示、灰度26~30用绿色显示等。
《图像处理技术》大作业学号:200808205135姓名:柯锦班级:B08082051学院:数计1 作业题目基于图像的动态景物的监测与跟踪2 作业数据短视频,背景相同,一个目标运动,及多目标运动3 基本方法(1)图像增强(2)图像基本运算(3)图像分割见代码注释:单目标主要代码如下function pushbutton4_Callback(hObject, eventdata, handles)% hObject handle to pushbutton4 (see GCBO)% eventdata reserved - to be defined in a future version of MATLAB% handles structure with handles and user data (see GUIDATA)global KeJin;%设置全局变量,用于记录目标单击的按钮axes(handles.axes1); %在axes1中处理,实现原动画的显示clear data %数据清理avi = aviread('samplevideo.avi');%读取一个avi动画,放到临时数组avi中video = {avi.cdata};for a = 1:length(video) %一帧一帧的读取video,并调用绘图函数imagesc显示每一帧的内容,以达到avi动画的播放imagesc(video{a});axis image offdrawnow;end;%handles.keJin=1;%guidata(hObject, handles);KeJin=1;% --- Executes on button press in pushbutton5.function pushbutton5_Callback(hObject, eventdata, handles)% hObject handle to pushbutton5 (see GCBO)% eventdata reserved - to be defined in a future version of MATLAB% handles structure with handles and user data (see GUIDA TA)global KeJin;if KeJin==1axes(handles.axes2);%在axes2中处理,实现目标的跟踪的显示clear dataavi = aviread('samplevideo.avi');video = {avi.cdata};tracking(video);%调用tracking函数,实现动画跟踪(单目标)endif KeJin==2axes(handles.axes2);MovingDetect();%调用MovingDetect函数,实现动画跟踪(多目标)endfunction d = tracking(video) %跟踪处理函数,实现一个运动目标的跟踪if ischar(video)% Load the video from an avi file.avi = aviread(video);pixels = double(cat(4,avi(1:2:end).cdata))/255;%将数据缩小255倍,然后以第四维进行组合,图像的增强(像素点的值缩小)clear avielse% Compile the pixel data into a single arraypixels = double(cat(4,video{1:2:end}))/255; %将数据缩小255倍,然后以第四维进行组合,图像的增强(像素点的值缩小)clear videoend% Convert to RGB to GRAY SCALE image.nFrames = size(pixels,4);%pixels第四维的大小放在nFrames中(总帧数)for f = 1:nFrames %对于没一帧,都做如下工作pixel(:,:,f) = (rgb2gray(pixels(:,:,:,f))); %转换为灰度图像endrows=240;cols=320;nrames=f;for l = 2:nrames%从第二帧到最后一帧,都做如下工作%下句为图像的基本运算,减运算,也是该跟踪的根本算法之一d(:,:,l)=(abs(pixel(:,:,l)-pixel(:,:,l-1)));%前后图像相减,得到的矩阵放到d中,以实现图像跟踪,此为图像的基本运算k=d(:,:,l);bw(:,:,l) = im2bw(k, .2);%转化为二值图像bw1=bwlabel(bw(:,:,l));imshow(bw(:,:,l))%显示二值图像hold on%右方向为横坐标轴%下方向为纵坐标轴cou=1;for h=1:rowsfor w=1:colsif(bw(h,w,l)>0.5)%此处用到图像分割,全局阀值分割toplen = h; %每一帧,如果cou!=1的话,下边界纵坐标放在toplen中,if (cou == 1)tpln=toplen; %每一帧,上边界纵坐标放在tpln中endcou=cou+1;breakendendend%disp(toplen);coun=1;for w=1:colsfor h=1:rowsif(bw(h,w,l)>0.5)%此处用到图像分割,全局阀值分割leftsi = w; %每一帧,如果coun!=1的话,右边界横坐标放在leftsi中if (coun == 1)lftln=leftsi; %每一帧,左边界横坐标放在lftln中coun=coun+1;endbreakendendendwidh=leftsi-lftln;%矩形的长(宽度)heig=toplen-tpln;%矩形的宽(高度)widt=widh/2;heit=heig/2;with=lftln+widt;%中心横坐标heth=tpln+heit;%中心纵坐标rectangle('Position',[lftln tpln widh heig],'EdgeColor','r');%用红色矩形框住目标,以确定目标位置plot(with,heth, 'r^');%用Δ标出目标的中心drawnow;hold offend;多目标function x = MovingDetect()clear data%初始化背景所需要的帧数N=15;%背景更新参数p1 = 95;p2 = 100-p1;M_ = 1;%读取A VI视频文件mov = aviread('My.AVI');%获取视频帧数dimension = size(mov);frameQTY = dimension(2);M=mov;tmFr = rgb2gray(frame2im(mov(1)));s = size(tmFr);scrsz = get(0,'ScreenSize');%%%h1 = figure('position', [scrsz(4)/2,scrsz(4)/2,s(2)*2,s(1)*2]); cdataSum = int16(zeros(s));sedisk = strel('rectangle',[2,2]);sedisk2 = strel('rectangle',[3,2]);count = zeros(s);for c1 = 1:frameQTY;colorFrC = M(c1);%获取第i帧frC = rgb2gray(frame2im(colorFrC));frC = im2frame(frC, gray(256));f = int16(frC.cdata);if( c1<= N)%前N帧用于背景建模movie(frC, 1, 15, [0,s(1),0,0]);%播放第i帧%movie(h1, frC, 1, 15, [0,s(1),0,0]);%播放第i帧cdataSum = cdataSum + f;else%开始运动检测,首先计算出背景if(c1==N+1)cdB = cdataSum/N;else cdB = int16(cdB);endf2 = abs( f - cdB );f2 = uint8(f2);if( c1 == 25)disp('1');endbwf = f2;bw = find( f2 < 45 );bwf(bw) = 0;cdB(bw) = (f(bw)+9*(cdB(bw)) )/10;bw = find( f2 >= 45 );bwf(bw) = 255;%bwf=logical(bwf);cdB = uint8(cdB);f3 = imdilate(bwf, sedisk);f3 = imerode(f3, sedisk);noSmall = bwareaopen( f3, 150 );%标签矩阵L = bwlabel( noSmall, 4 );taggedCars( :,: ) = frC.cdata;if any( L(:) )stats = regionprops(L,'boundingbox','filledArea');for label = 1:length(stats)box = stats(label);if( box.FilledArea < 200 || box.FilledArea/(s(1)*s(2)) > 0.3 )continue;endbox = box.BoundingBox;left=round(box(1));top=round(box(2));right=round(box(1)+box(3)-1);bottom=round(box(2)+box(4)-1);if( abs(top - bottom)/abs(right - left) > 4 || abs(right - left)/abs(top - bottom) > 4 )continue;endtaggedCars(top,left:right)=1; %画矩形框%taggedCars(bottom,left:right)=1;taggedCars(top:bottom,left)=1;taggedCars(top:bottom,right)=1;endend%f5 = imdilate(imerode(f,tk1), tk);ff = [frC.cdata, cdB;f3, taggedCars];%noSmall, taggedCarsff = im2frame(ff,gray(256));movie(ff, 1, 15, [0,0,0,0]);%播放第i帧%movie(h1, ff, 1, 15, [0,0,0,0]);%播放第i帧end% end of if-elseend%--播放结束4 作业完成目标:动态目标的定位与跟踪,并用方框提示并给出运动轨迹截图如下:单目标:多目标:部分代码来源于:/search.php?searchid=475&orderby=lastpost&ascdesc=desc&searchsubmit=yes&page=1小结:此次大作业采用减法运算达到单个目标跟踪的目的,采用了图像增强,设定阀值使结果便于观察,分析。
实验报告实验课程:数字图像处理实验名称:骨髓细胞计数实验地点:计算机中心405学生姓名:学号:指导教师:实验时间:年月一、实验要求:将被染成紫色的白细胞从较暗的红细胞和背景中分割出来,去除细胞粘连,用数字图像处理技术实现图像中紫色细胞的自动计数。
二、实验过程1、对原图像进行预处理(1)由于源图像是彩色图像,为了便于进行仿真实验,在进行处理之前需要将彩色图像转换为灰度图像。
用到的函数为rgb2gray(2)获取图像的全局阈值并将图像二值化,以方便后面实验中对图像进行形态学修饰的进行(3)在图像获取过程中,由于热传输等影响,会产生高斯噪声,较适合采用中值滤波的方法进行去噪处理。
因为,中值滤波的方法能够较好的保护细胞的边缘信息,满足本实验的需求;(4)删除小面积图像(5)对图像进行开闭运算。
开和闭这两种运算可以除去比结构元素小的特定图像细节,同时保证不产生全局几何失真。
开运算可以把比结构元素小的突刺滤掉,切断细长搭接而起到分离作用;闭运算可以把比结构元素小的缺口或孔填充上,搭接短的间隔而起到连接作用。
(6)对图像进行孔洞填充,(7)开闭运算会留下一些偏离的孤立像素,使用bwareaopen来移除少于特定像素个数的斑点。
BW2= bwareaopen(BW,P)从二值图像中移除所以少于P像素值的连通块,得到另外的二值图像BW3。
实验图片显示如下:2、分割计数本次实验采用分水岭法对进行过预处理的图像进行分割和计数,最后计数得紫色细胞个数为35三、实验心得体会通过这学期的学习,我获益匪浅,学到了很多有趣的知识,知道了什么是灰度图,二值化,阈值,分水岭等很多有趣的图像处理方法,知道了怎么使用matlab帮助查找自己不会的函数,也成功的自己独立完成了细胞的计数。
这门课也激起了我对图像处理的兴趣,课程结束后也会继续学习更多的函数知识,不会停止这方面的学习。
四、源代码Image = imread('b1.bmp'); subplot(321);imshow(Image);title('原图');Image=rgb2gray(Image);subplot(322);imshow(Image);title('灰度图');Theshold = graythresh(Image); BW = im2bw(Image,1.3*Theshold);subplot(323),imshow(BW);title('二值化图像');BW1= medfilt2(BW,[13 13]);BW1=bwareaopen(BW,50,4)subplot(3,2,4);imshow(BW1);title('删除小面积后图像');se=strel('disk',5);fo=imopen(BW1,se);foc=imclose(fo,se)subplot(3,2,5);imshow(foc);title('开闭运算后图像');BW2=imfill(foc,'holes');subplot(3,2,6);imshow(BW2);title('孔洞填充后图像');BW3=bwareaopen(BW2,20);D=bwdist(~BW3);D=-D;D(~BW3)=-Inf;L=watershed(D);rgb=label2rgb(L,'jet');figure;imshow(rgb,[]);title('分水岭分割计算后图像');number=max(max(L))-1; number方法一源程序:A=imread('y1.bmp');B=rgb2gray(A);h=[0 1 0,1 -4 1,0 1 0];J1=conv2(B,h,'same');K1=medfilt2(J1,[3 3]);¨BW=edge(K1,'canny');SE= strel('line',11,90);BW1=imdilate(BW,SE);BW2=bwmorph(BW1,'thin',Inf);[H, theta, rho] = hough(BW2, 'ThetaResolution', 0.2);subplot(221),imshow(H, [], 'XData', theta, 'YData', rho, 'InitialMagnification', 'fit')axis on, axis normalxlabel('\theta'), ylabel('\rho') ,peaks = houghpeaks(H, 5);hold onplot(theta(peaks(:, 2)), rho(peaks(:, 1)), ...'linestyle', 'none', 'marker', 's', 'color', 'w');lines = houghlines(BW2, theta, rho, peaks);subplot(222),imshow(BW2), hold onfor k = 1:length(lines)xy = [lines(k).point1 ; lines(k).point2];plot(xy(:,1), xy(:,2), 'LineWidth', 4, 'Color', [.8 .8 .8]); end。
2014年上学期《数字图像处理》复习大作业及参考答案=====================================================一、选择题(共20题)1、采用幂次变换进行灰度变换时,当幂次取大于1时,该变换是针对如下哪一类图像进行增强。
( B)A 图像整体偏暗B 图像整体偏亮C图像细节淹没在暗背景中 D图像同时存在过亮和过暗背景2、图像灰度方差说明了图像哪一个属性。
( B )A 平均灰度B 图像对比度C 图像整体亮度 D图像细节3、计算机显示器主要采用哪一种彩色模型( A )A、RGBB、CMY或CMYKC、HSID、HSV4、采用模板[-1 1]T主要检测( A )方向的边缘。
A.水平B.45︒C.垂直D.135︒5、下列算法中属于图象锐化处理的是:( C )A.低通滤波B.加权平均法C.高通滤波D. 中值滤波6、维纳滤波器通常用于( C )A、去噪B、减小图像动态范围C、复原图像D、平滑图像7、彩色图像增强时, C 处理可以采用RGB彩色模型。
A. 直方图均衡化B. 同态滤波C. 加权均值滤波D. 中值滤波8、__B__滤波器在对图像复原过程中需要计算噪声功率谱和图像功率谱。
A. 逆滤波B. 维纳滤波C. 约束最小二乘滤波D. 同态滤波9、高通滤波后的图像通常较暗,为改善这种情况,将高通滤波器的转移函数加上一常数量以便引入一些低频分量。
这样的滤波器叫 B。
A. 巴特沃斯高通滤波器B. 高频提升滤波器C. 高频加强滤波器D. 理想高通滤波器10、图象与灰度直方图间的对应关系是 B __A.一一对应B.多对一C.一对多D.都不11、下列算法中属于图象锐化处理的是: CA.低通滤波B.加权平均法C.高通滤D. 中值滤波12、一幅256*256的图像,若灰度级数为16,则存储它所需的比特数是:( A )A、256KB、512KC、1M C、2M13、噪声有以下某一种特性( D )A、只含有高频分量B、其频率总覆盖整个频谱C、等宽的频率间隔内有相同的能量D、总有一定的随机性14. 利用直方图取单阈值方法进行图像分割时:(B)a.图像中应仅有一个目标b.图像直方图应有两个峰c.图像中目标和背景应一样大d. 图像中目标灰度应比背景大15. 在单变量变换增强中,最容易让人感到图像内容发生变化的是( C )A亮度增强觉 B饱和度增强C色调增强 D不一定哪种增强16、利用平滑滤波器可对图像进行低通滤波,消除噪声,但同时模糊了细节。
实验报告
实验课程:数字图像处理
实验名称:骨髓细胞计数实验地点:计算机中心405
学生姓名:
学号:
指导教师:
实验时间:年月
一、实验要求:
将被染成紫色的白细胞从较暗的红细胞和背景中分割出来,去除细胞
粘连,用数字图像处理技术实现图像中紫色细胞的自动计数。
二、实验过程
1、对原图像进行预处理
(1)由于源图像是彩色图像,为了便于进行仿真实验,在进行处理之前需要将彩色图像转换为灰度图像。
用到的函数为rgb2gray
(2)获取图像的全局阈值并将图像二值化,以方便后面实验中对图像进行形态学修饰的进行
(3)在图像获取过程中,由于热传输等影响,会产生高斯噪声,较适合采用中值滤波的方法进行去噪处理。
因为,中值滤波的方法能够较好的保护细胞的边缘信息,满足本实验的需求;(4)删除小面积图像
(5)对图像进行开闭运算。
开和闭这两种运算可以除去比结构元素小的特定图像细节,同时保证不产生全局几何失真。
开运算可以把比结构元素小的突刺滤掉,切断细长搭接而起到分离作用;闭运算可以把比结构元素小的缺口或孔填充上,搭接短的间隔而起到连接作用。
(6)对图像进行孔洞填充,
(7)开闭运算会留下一些偏离的孤立像素,使用bwareaopen来移除少于特定像素个数的斑点。
BW2= bwareaopen(BW,P)从二值图像中移除所以少于P像素值的连通块,得到另外的二值图像BW3。
实验图片显示如下:
2、分割计数
本次实验采用分水岭法对进行过预处理的图像进行分割和计数,最后计数得紫色细胞个数为35
三、实验心得体会
通过这学期的学习,我获益匪浅,学到了很多有趣的知识,知道了什么是灰度图,二值化,阈值,分水岭等很多有趣的图像处理方法,知道了怎么使用matlab帮助查找自己不会的函数,也成功的自己独立完成了细胞的计数。
这门课也激起了我对图像处理的兴趣,课程结束后也会继续学习更多的函数知识,不会停止这方面的学习。
四、源代码
Image = imread('b1.bmp'); subplot(321);
imshow(Image);
title('原图');
Image=rgb2gray(Image);
subplot(322);
imshow(Image);
title('灰度图');
Theshold = graythresh(Image); BW = im2bw(Image,1.3*Theshold);
subplot(323),imshow(BW);
title('二值化图像');
BW1= medfilt2(BW,[13 13]);
BW1=bwareaopen(BW,50,4)
subplot(3,2,4);
imshow(BW1);
title('删除小面积后图像');
se=strel('disk',5);
fo=imopen(BW1,se);
foc=imclose(fo,se)
subplot(3,2,5);
imshow(foc);
title('开闭运算后图像');
BW2=imfill(foc,'holes');
subplot(3,2,6);
imshow(BW2);
title('孔洞填充后图像');
BW3=bwareaopen(BW2,20);
D=bwdist(~BW3);
D=-D;
D(~BW3)=-Inf;
L=watershed(D);
rgb=label2rgb(L,'jet');
figure;
imshow(rgb,[]);
title('分水岭分割计算后图像');
number=max(max(L))-1; number
方法一源程序:
A=imread('y1.bmp');
B=rgb2gray(A);
h=[0 1 0,1 -4 1,0 1 0];
J1=conv2(B,h,'same');
K1=medfilt2(J1,[3 3]);¨
BW=edge(K1,'canny');
SE= strel('line',11,90);
BW1=imdilate(BW,SE);
BW2=bwmorph(BW1,'thin',Inf);
[H, theta, rho] = hough(BW2, 'ThetaResolution', 0.2);
subplot(221),imshow(H, [], 'XData', theta, 'YData', rho, 'InitialMagnification', 'fit')
axis on, axis normal
xlabel('\theta'), ylabel('\rho') ,
peaks = houghpeaks(H, 5);
hold on
plot(theta(peaks(:, 2)), rho(peaks(:, 1)), ...
'linestyle', 'none', 'marker', 's', 'color', 'w');
lines = houghlines(BW2, theta, rho, peaks);
subplot(222),imshow(BW2), hold on
for k = 1:length(lines)
xy = [lines(k).point1 ; lines(k).point2];
plot(xy(:,1), xy(:,2), 'LineWidth', 4, 'Color', [.8 .8 .8]); end。