赛默飞世尔Corona Ultra系列CAD检测器样本

赛默飞近红外参数【实用版】目录一、赛默飞近红外光谱仪的概述二、赛默飞近红外光谱仪的参数1.Nicolet iS 5N2.Trudefender 手持红外光谱仪三、赛默飞近红外光谱仪的应用领域四、赛默飞近红外光谱仪的优势五、结语正文一、赛默飞近红外光谱仪的概述赛默飞近红外光谱仪是一款高性能的光谱分析仪器，能够对样品进行近红外区域的光谱分析，被广泛应用于各个领域，如化学、生物学、医学、环境监测等。

赛默飞作为全球知名的科学仪器制造商，在近红外光谱仪领域有着丰富的经验和技术积累。

二、赛默飞近红外光谱仪的参数1.Nicolet iS 5Nicolet iS 5N 是赛默飞一款先进的近红外光谱仪，具有高分辨率、高灵敏度和快速扫描等特点。

其主要参数如下：- 波长范围：0.9μm ~ 1.7μm- 分辨率：≤0.5cm^-1- 灵敏度：≤0.001g/mL- 扫描速度：≤1s/点2.Trudefender 手持红外光谱仪Trudefender 是赛默飞一款手持式近红外光谱仪，具有便携、易操作和实时分析等特点。

其主要参数如下：- 波长范围：0.9μm ~ 1.7μm- 分辨率：≤1.5cm^-1- 灵敏度：≤0.01g/mL- 扫描速度：≤2s/点- 重量：≤3kg三、赛默飞近红外光谱仪的应用领域赛默飞近红外光谱仪广泛应用于各种领域，如生物医学、化学化工、食品饮料、环境和材料科学等。

在生物医学领域，可以用于蛋白质结构分析、疾病诊断和生物分子识别等；在化学化工领域，可以用于分子结构分析、化学反应监测和产品质量控制等；在食品饮料领域，可以用于成分分析、品质控制和食品安全监测等。

四、赛默飞近红外光谱仪的优势赛默飞近红外光谱仪具有以下优势：1.高分辨率和高灵敏度，能够对样品进行精确分析。

2.快速扫描和实时分析，能够提高分析效率。

3.便携式设计，便于携带和现场分析。

4.丰富的应用经验和技术支持，确保分析结果的准确性。

五、结语赛默飞近红外光谱仪凭借其优异的性能和广泛的应用领域，在光谱分析领域具有重要地位。

5 µmCu Ag O CaSi Microstructure of copper-silver alloy revealed with ChemiSEM technology. Silicate contamination is immediately recognized when inspecting samples with live compositional imaging via ChemiSEM.2 µmApreo 2 SEMUnmatched versatility powered by ChemiSEM TechnologyDatasheetResolve gray areas with the Thermo Scientific Apreo 2 SEM, a high-performance field emission gun (FEG) SEM with unique, live elemental imaging and an advanced, automated optics system that enables you to focus on your research rather than microscope performance.Key featuresAll-round nanometer or sub-nanometer resolution performance on materials ranging from nanoparticles,powders, catalysts, and nanodevices to bulk magnetic samples, even at long (10 mm) working distancesExtreme flexibility for handling a wide range of sampletypes, including insulators, sensitive materials, or magnetic samples, and for collecting the data that matters most to your applicationLess time spent on maintenance with an optics system that tiling, and stitchingChemiPhase image showing different phases present in a complex inclusion in steel.P3P2P4The result is an easy-to-use system that allows you to focus on discovery rather than manipulating multiple software packages.The Apreo SEM’s unique Trinity in-column detection systemis present, but now with improved performance. TheApreo 2 SEM remains the platform of choice for research on nanoparticles, catalysts, powders, and nanodevices, thanksto its innovative final lens design that does not compromise on magnetic sample imaging performance. The electrostatic final lens (available on Apreo 2 C and Apreo 2 S SEMs) enables simultaneous in-column detection at high resolution, whilethe Apreo 2 S SEM combines the electrostatic final lens with magnetic immersion into a compound lens. The compound final lens further boosts resolution performance, providinga resolution of 0.9 nm at 1 kV without additional beam deceleration, while offering unique options for signal filtering.For the most challenging applications, the Apreo 2 SEM’s charge mitigation routines can include optional low vacuum (up to 500 Pa) to mitigate charge on any sample while providing excellent resolution and large analytical currents with field-proven through-the-lens differential pumping and dedicated LoVac detectors.All these capabilities are complemented by easy sample handling and an easy-to-use microscope user interface, saving time for novice and expert users alike. A customizable user interface provides many options for user guidance, automation, and remote operation. With unique technologies like SmartAlign, FLASH, and ChemiSEM Technology addedto an already advanced microscope, the Apreo 2 SEM adds additional flexibility to any lab while providing advanced imaging capability for all users.Electron optics• High-resolution field emission SEM column with:–High-stability Schottky field emission gun to provide stable high-resolution analytical currents–Compound final lens: a combined electrostatic, field-free magnetic and immersion magnetic objective lens(optional)–60° objective lens geometry: allows tilting larger samples –Automated heated apertures to ensure cleanliness and touch-free aperture changes• SmartAlign Technology: user-alignment-free technology • Through-the-lens differential pumping for low vacuum (optional) reduces beam skirting for the most accurateanalysis and highest resolution• Beam deceleration with stage bias from -4,000 V to +600 V • Continuous beam current control and optimized aperture angle • Double stage scanning deflection• Easy gun installation andmaintenance: auto bake-out, autostart, no mechanical alignments• PivotBeam Mode for selected areaelectron channeling, also known as“rocking beam” mode (Apreo 2 Smodel only)• Guaranteed minimum source lifetime: 24 monthsElectron beam resolutionElectron beam parameter space• Beam current range: 1 pA to 50 nA(400 nA configuration also available)• Accelerating voltage range: 200 V – 30 kV• Landing energy range: 20 eV – 30 keV• Max. horizontal field width: 3 mm at 10 mm WD (corresponds to 29x minimum magnification)Chamber• Inner width: 340 mm• Analytical working distance: 10 mm• Ports: 12• EDS take-off angle: 35°• Three simultaneous EDS detectors possible, two at 180°•Coplanar EDS/EBSD orthogonal to the tilt axis of the stageApreo 2 C Apreo 2 S15 kV (30 Pa) 1.2 nm 1.2 nmBD: beam deceleration mode. WD: working distance. Resolutions are at optimum working distance unless specified otherwise. By default, upon final installation,the resolution is proven in the systems acceptance test at 1 kV and 30 kV in highvacuum and with immersion switched on if applicable.DetectorsThe Apreo 2 SEM detects up to four signals simultaneously from any combination of the available detectors or detector segments (optional):• Trinity Detection System (in-lens and in-column)–T1 segmented lower in-lens detector–T2 upper in-lens detector–T3 in-column detector (optional)• ETD—Everhart-Thornley SE detector• DBS—Retractable segmented under-the-lens BSED (optional)• Low-vacuum SE detector (optional)• DBS-GAD—Lens-mounted gaseous analytical BSED (optional)• STEM 3+—Retractable segmented detector(BF, DF, HADF, HAADF) (optional)• IR-CCD• Thermo Scientific Nav-Cam™ Camera (chamber-mounted)ChemiSEM Technology (optional)• EDS detector size: 10, 30, or 60 mm²• Light element sensitivity down to beryllium• 127 eV or 129 eV spectral resolution• Optional motorized slide availableVacuum system• Complete oil-free vacuum system• 1 × 240 l/s TMP• 1 × PVP-scroll• 2 × IGP• Chamber vacuum (high vacuum) <6.3 × 10-6 mbar (after 12 hours pumping)• Evacuation time: ≤3.5 minute• Optional low-vacuum mode• 10–500 Pa chamber pressure• Automatic Pressure Limiting Aperture (PLA) LoaderSample holders• Standard multi-purpose holder uniquely mounts directly onto the stage, hosts up to 18 standard stubs (ø12 mm),three pre-tilted stubs, cross-section samples, and two pre-tilted row-bar holders (optional) (38° and 90°). Tools are not required to mount a sample.• Each optional row-bar accommodates 6 STEM grids• Wafer and custom holders (optional)System control• 64-bit GUI with Windows 10, keyboard, optical mouse • 24-inch LCD display, WUXGA 1920×1200(second monitor optional)• Customizable graphical user interface,with up to 4 simultaneously active views• FLASH automated image tuning for focus,lens align, and stigmator• Image registration• Navigation montage• Image analysis software• Undo / Redo functionality• User guidance for basic operations / applications• Optional joystick• Optional manual user interface (knob board)Image processor• Dwell time range from 25 ns to 25 ms/pixel• Up to 6144×4096 pixels• File type: TIFF (8-, 16-, 24-bit), JPEG or BMP• Single-frame or 4-view image display• SmartScan Mode (256-frame average or integration, line integration and averaging, interlaced scanning)• DCFI (drift compensated frame integration) Mode• Digital image improvement and noise reduction filter Type Eucentric goniometer stage,5 axes motorizedXY110x110 mmRepeatability <3.0 μm (@ 0° tilt)Motorized Z65 mmRotation n × 360°Tilt -15° / +90°Max. sample height Clearance 85 mm to eucentric point Max. sample weight 500 g in any stage positionUp to 5 kg at 0° tiltMax. sample size122 mm diameter with fullX, Y, rotation (larger samplespossible with limited stagetravel or rotation)For research use only. Not for use in diagnostic procedures. For current certifications, visit /certifications© 2023 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified. DS0345-EN-07-2023Accessories (optional)• Sample / chamber cleaning: CryoCleaner,Integrated Plasma Cleaner• Analysis: EDS, EBSD, WDS, CL, Raman• Thermo Scientific QuickLoader ™Load Lock for fastsample transfer• Navigation: correlative navigation, Thermo Scientific Maps ™Software tiling and stitching• Gas injection: up to 2 units (other accessories may limitnumber of GIS available) for beam-induced deposition of:–Platinum –Tungsten –Carbon • Manipulators • Cryo-stage• Electrical probing / multi-probing stations • Electrostatic beam blanker• CleanConnect Sample Transfer DeviceSoftware options• Maps Software for automatic large area acquisition usingtiling and stitching; correlative work• Thermo Scientific AutoScript ™ 4 Software—Python-basedapplication programming interface• TopoMaps for image colorization, image analysis,and 3D surface reconstruction• Advanced image analysis software • Remote control softwareDocumentation• Online user guidance• Operating instructions handbook • Online help• Prepared for RAPID (remote diagnostic support)• Free access to online resources for ownersWarranty and Training• 1 year warranty• Choice of service maintenance contracts• Choice of operation / application training contractsInstallation requirement(Refer to preinstall guide for detailed data)• Power:–Voltage 100–240 V AC (-6%, +10%) –Frequency 50 or 60 Hz (±1%)–Consumption: <3.0 kVA for basic microscope • Earth resistance <0.1 Ω• Environment:–Temperature (20 ± 3)°C –Relative humidity below 80%–Stray AC magnetic fields <40 nT asynchronous, <100nT synchronous for line times, 20 ms (50 Hz mains) or 17 ms (60 Hz mains)• Minimum door size: 0.9 m wide × 1.9 m high • Weight: column console 980 kg • Dry nitrogen recommended for venting • Compressed air 4–6 bar, clean, dry and oil-free • System chiller• Acoustics: site survey required,as acoustic spectrum relevant• Floor vibrations: site survey required,as floor spectrum relevant• Optional active vibration isolation tableConsumables (partial list)• Replacement Schottky electron source moduleL earn more at /apreo。

HPLC-CAD法检测庆大霉素C 组分引言氨基糖苷类抗生素的测定由于在色谱柱上保留弱，没有紫外吸收，对于其含量的测定一直是一个难点。

蒸发光检测器可用于该类化合物的测定，但蒸发光线性、响应一致性和稳定性均较差，一直让其使用者感到较难接受。

电喷雾检测器基于其自身技术特点，在动态响应和稳定性上有了很大的提高，近年来深受广大科研工作者欢迎。

本文使用电喷雾检测器来测定庆大霉素注射液中的有效组分，使广大用户对该检测器的应用有个更好的认识。

测定条件Ultimate 3000系列：泵：DGP-3400自动进样器：WPS-3000SL柱温箱：TCC-3000柱温：30℃检测器：Corona Ultra CAD检测器条件：CAD: neb=25℃ Gas pressure=35.0psi ； 进样量：10 μL ；色谱柱：Acclaim C18 5 μm, 4.6×150mm (PN 059149)流动相：100mM TFA/H2O —MeOH (92:8)流速：0.6 mL/min样品前处理对照品溶液制备精密称定一定量的庆大霉素对照品，加流动相溶解并定量稀释成1mg/ml 的对照品溶液，精密吸取上述溶液10µL 注入液相色谱仪进行测定。

供试品溶液制备将庆大霉素注射液(2ml 8万单位)用流动相稀释成相当于含庆大霉素0.8mg/ml 的供试品溶液，摇匀，0.22µm 滤膜滤过，精密吸取上述溶液10µL 注入液相色谱仪进行测定。

结果和讨论庆大霉素对照品测定谱图(1mg/ml ，进样10µL)样品测定谱图(0.8mg/ml 注射液，进样10µL)刘兴国 金燕赛默飞世尔科技（中国）有限公司线性关系不取对数4个组分在(0.1mg/ml 到2mg/ml)20倍的动态范围内线性良好样品测定结果样品 C1a% c2% c2a% c1% (c2+c2a)%1 25.63807 24.40509 23.85764 26.12537 48.262732 25.65109 24.53197 23.86917 26.16714 48.40114该样品C 组分合格，符合C1 25%-50%，C1a 15%-40%，(c2+c2a)% 20%-50%的药典规定。

CAD VEO操作注意事项1.目的规范CAD VEO液相色谱检测器开关机注意事项，正确使用仪器，保证检测工作顺利进行、操作人员人身安全和设备安全。

2.适用范围适用于CAD VEO液相色谱检测器的使用操作。

3.职责3.1 CAD VEO液相色谱检测器操作人员应严格按照本规程进行仪器的开关机，对仪器进行正确地日常维护，并填好使用记录。

3.2 CAD VEO液相色谱检测器保管人员负责监督仪器操作是否符合规程，对仪器进行正确地开关机、定期维护、保养。

3.3 室主任负责仪器综合管理。

4．开关机操作程序4.1 开机注意事项4.1.1 确认氮气钢瓶压力，开启氮气压力到0.6Mpa,确保持续供气。

气体压力不可过高,否则CAD进气口容易冲开。

如果使用发生器系统，开启发生器电源。

开启出口通风设备。

如果出口废气管排放到室外可以不用.4.1.2 开启VEO电源。

启动液相色谱系统。

4.1.3 打开电脑。

打开变色龙软件。

选择仪器界面。

点击CAD选项卡，到控制界面。

4.1.4 选择打开GAS ON。

选择检测界面里的more option。

设置雾化器温度（35或者50度，RS型号可以具体设定到室温+5度-100度）。

对难挥发的化合物，提高雾化器温度，有利于降低背景噪音。

4.1.5 开机通氮气的时间越长，CAD的灵敏度越高，一般在4小时左右达到稳定值，建议开机通氮气的时间为4小时。

4.1.6 连接CAD之前，将液相系统冲洗干净。

如果不确定流路是否干净，请断开检测器入口流路。

冲洗整个系统。

如果是Veo RS型号，请直接选择detector flow 到off状态。

冲洗完成后，Veo型号接回检测器入口管路。

Veo Rs型号直接选择detector flow到on状态。

4.1.7 用流动相平衡色谱柱。

冲洗时间大于等于15-20倍柱体积。

注意：流动相只能使用挥发性溶剂和挥发性缓冲盐和酸，如三氟乙酸，甲酸，乙酸，甲酸铵和乙酸铵。

在不影响色谱行为的情况下，选用选用甲酸铵做流动相添加剂可以获得比同浓度乙酸铵更低的背景噪音。

HPLC—CAD法同时测定葡萄糖氯化钠注射液中葡萄糖和氯及钠的含量目的：建立高效液相色谱-电喷雾检测器（HPLC-CAD）法同时测定葡萄糖氯化钠注射液中葡萄糖、氯和钠的含量。

方法：采用Thermo U3000-Corona CAD 测定，用超纯水溶解葡萄糖氯化钠注射液，过滤后注入液相色谱仪测定。

色谱柱为Thermo Mixed-Mode Hilic-1柱（3μm 21×150mm）；柱温：40℃；流动相：50mmol/L醋酸铵和乙腈梯度洗脱；流速：03mL/min；进样体积：10μL。

结果：葡萄糖的线性范围为005～100mg/mL（r=09993），氯和钠的线性范围为009～180mg/mL（r=09999）。

方法回收率均>998%，精密度RSD均<20%，超纯水中ClO-、NO3-对葡萄糖氯化钠的含量测定几乎没有干扰。

结论：该方法准确，快速、简便，可用于葡萄糖氯化钠注射液中葡萄糖、氯和钠的测定及其质量控制，对其类似化合物的测定具有参考价值。

标签：葡萄糖氯化钠注射液；高效液相色谱；电喷雾检测器Determination of Glucose，Cl- and Na+ in Glucose and Sodium Chloride Injectionby High Performance Liquid Chromatography with Charge Aerosol Detection（HPLC-CAD）SUN LiangguangGUO Zhenwang*Guangxi Wuzhou Institute for Food and Drug Control，Wuzhou 543001，ChinaAbstract：Objective To establish a high performance liquid chromatographic with charge aerosol detection（HPLC-CAD）method for the determination of Glucose，Cl- and Na+ in Glucose and sodium chloride injection Methods An Thermo U-3000 HPLC system with a charged aerosol detector was usedThe Glucose and sodium chloride injection samples were dissolved by water and injected into a Thermo Mixed-Mode Hilic-1（3μm 21×150mm）column at 40℃The mobile phase was composed of 50 mmol·L-1 ammonium acetate-acetonitrile with a flow rate of 03mL·min-1The injection volumn was 10μL Results The methodology recovery was higher than 998%The RSD were less than 20%The calibration curve was linear at 005～100mg·mL-1，with coefficient of determination over 09999Glucose and sodium chloride injection was detected with little interference from the excipient blend of waterConclusion The method is accurate，rapid and convenient for releage and stability test of Glucose，Cl- and Na+ in Glucose and sodium chloride injection and may be used as reference for similar compoundsKeywords：Glucose and Sodium Chloride Injection；HPLC；CAD葡萄糖氯化鈉注射液（Glucose and sodium chloride injection）是常用的补充热能和体液的复方制剂，内含葡萄糖与氯化钠，用于各种原因引起的进食不足或大量体液丢失[1]。

HPLC-CAD法测定硫酸新霉素中新霉素B、新霉素C、新霉胺及其他有关物质刘庄蔚;朱健萍;梁秋霞;蒋洁;杨欣智【摘要】目的建立测定硫酸新霉素中新霉素B、新霉素C、新霉胺及其他有关物质的高效液相色谱-电喷雾检测器(HPLC-CAD)方法.方法采用Waters HSS T3色谱柱(4.6mm×250mm,5μm),以0.15mol/L三氟乙酸水溶液为流动相,流速1.0mL/min,柱温30℃,电喷雾检测器的雾化温度为45℃.结果新建方法对新霉素B、新霉素C、其他有关物质分离度良好,精密度、重复性和回收率均满足分析要求.新霉素B、新霉胺浓度与峰面积线性关系良好(r＞0.995),检出限可达到0.02μg和0.003μg.结论新建方法分离度好、灵敏度高,可以检出更多的杂质,可以满足硫酸新霉素原料有关物质分析的要求.【期刊名称】《中国抗生素杂志》【年(卷),期】2019(044)003【总页数】6页(P370-375)【关键词】电喷雾检测器;硫酸新霉素;有关物质;高效液相色谱法【作者】刘庄蔚;朱健萍;梁秋霞;蒋洁;杨欣智【作者单位】广西壮族自治区食品药品检验所,南宁530021;广西壮族自治区食品药品检验所,南宁530021;广西壮族自治区食品药品检验所,南宁530021;广西壮族自治区食品药品检验所,南宁530021;广西壮族自治区食品药品检验所,南宁530021【正文语种】中文【中图分类】R978.1;R917硫酸新霉素(neomycin sulfate)是首个被发现的氨基糖苷类抗生素，它是1949年Waksman等[1]从新霉素链霉菌代谢产物中分离得到的混合物。

其抗菌谱较广，对革兰阳性菌、革兰阴性菌、结核杆菌等均有抑制作用[2]，由于新霉素有显著的肾毒性和耳毒性，故常被制备成乳膏剂、软膏剂和滴眼液等外用制剂供患者使用。

据文献报道，硫酸新霉素的主要成分为新霉素A、B、C 3种，新霉素A(新霉胺)仅微量，是新霉素B、C的降解产物之一[1]。

四极式离子阱 GC/MS nITQ™ 系列为真实世界的样品分析而优化设计●最可靠的离子阱技术●无可匹敌的全扫描灵敏度●先进的MSn扫描对复杂基质样品提供高的选择性●外离子源能够得到最大的生产率、可靠性，以及经典的谱库检索质谱图Thermo Scienti fic ITQ 系列外源离子阱质谱仪是得到工业界认可的传统离子阱仪器中最新的一代—GC/MS n 。

能提供无与伦比的分析结果，可承受满负荷的全天工作 — 无论是监测工业企业生产设备附近水质量的环境实验室，还是为调查犯罪做线索追踪的国家法院实验室，或者是为全球性经济力争保护食品的食品安全实验室，ITQ 系列都能满足您的分析需要。

Thermo Scienti fic ITQ 系列四极式离子阱当前最高灵敏度的离子阱质谱仪ITQ 系列GC/MS n 仪器给你的实验室提供选择的机会，它允许你根据你实验室的需求选择相匹配的GC/MS 系统。

• Thermo Scienti fic ITQ 700™: 常规实验室的理想选择，它适合于空间和预算都有限的实验室做GC/MS 全扫描分析。

• Thermo Scienti fic ITQ 900™:灵活进样口和检测器的选择，进一步扩大了GC 的灵活性。

• Thermo Scienti fic ITQ 1100™: 从研究到常规应用的最终选择，它具有强大的新工具程序，能够扩展你实验室的能力。

ITQ 系列以提高性能的理念而设计，通过不断适应随着时间的推移而逐渐改变的工作流程和需要，来节省您的投资。

如果你需要改变，升级你的仪器可获得更新的性能、更高灵活度和更强大的功能。

更好的是，无论如何选择, 你都会得到可用的灵敏性最好的GC-离子阱质谱仪，即使复杂的基质，也会获得很低的检出限。

ITQ 系列提供了一系列的操作模式， 从全扫描MS 和MS/MS （MS n ），到正/负化学离子化。

连续全扫描和MS/MS 的双模式或者正离子/负离子化学离子源(PPINICI ™)*允许我们单次进样获得两种形式的数据。