LeicaDMILM倒置金相显微镜-金相显微镜-USFEN

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leica dmi8倒置荧光显微镜工作原理
Leica DMI8倒置荧光显微镜工作原理基于倒置的光路径设计，通过逆向放大观察样品。

具体工作原理如下：
1. 光源：Leica DMI8倒置荧光显微镜通常采用激光或者弧光
灯作为光源。

光线通过光源进入显微镜系统。

2. 激发光源与滤光片：激发光源产生的光通过滤光片进行过滤。

滤光片选择能够激发样品荧光的波长，而过滤其他多余的光线。

3. 物镜：经过滤光片后的光线进入物镜。

物镜有不同的倍数，可以放大样品。

光在物镜中被聚焦，形成一个局部的高强度光点。

4. 分光镜：经过物镜后的光线进入分光镜。

分光镜表面涂有特殊的薄膜，将激发光和荧光信号分开。

激发光线被反射到目镜或相机，荧光信号则透过分光镜射向样品。

5. 样品：样品通常是放置在一个液体培养皿或者玻璃底片上。

荧光染料标记的生物标本或者细胞，会在激发光的作用下发出荧光信号。

6. 荧光信号：样品中的荧光染料吸收激发光子，高能激发光子的能量会导致荧光染料的电子跃迁到一个高能态，当电子回到基态时，会释放出激光波长较长的荧光信号。

7. 探测荧光信号：荧光信号通过目镜或者相机进行观察和记录。

荧光信号的特点可以提供有关样品的信息，如位置、结构和代谢活性等。

通过上述步骤，Leica DMI8倒置荧光显微镜能够通过荧光信
号对样品进行观察和分析。

徕卡光学显微镜操作方法徕卡光学显微镜是一种高质量的显微镜，使用起来需要一些操作方法和技巧。

下面，我将为您详细介绍徕卡光学显微镜的操作方法。

第一步：调节光源1. 打开显微镜底部的电源开关，通过转动亮度调控旋钮来调节光源的亮度。

2. 使用多种光源如白光、蓝光、绿光等，可以根据所观察的材料和需要来选择合适的光源，并通过切换按钮进行调节。

第二步：准备样本1. 取出待观察的样本，并使用切片机或切片刀等工具将样本切割成适当大小的薄片。

2. 将样本置于载物台上，通过定位螺丝将样本固定在载物台上，确保样本位置合适。

第三步：调焦1. 打开眼镜盒，并将目镜与眼镜盒上的刻度对齐。

2. 通过调节观察管的升降装置，将目镜与样本尽可能靠近，但不接触样本，然后通过调节调焦轮转动机构，使样本清晰可见。

3. 要注意调节焦距时，先用粗焦轮将样本基本对焦，再通过微调焦轮进行精细调节。

第四步：调节放大倍数1. 徕卡光学显微镜通常使用10倍至100倍的倍率，通过旋转目镜上的物镜切换器或者直接换上不同倍数的物镜，可以改变显微镜的放大倍数。

2. 每次切换倍率后，都需要重新调节焦距以保持样本的清晰可见。

第五步：调节光源方向和强度1. 通过显微镜底部的光源调节旋钮，可以调节光源的方向和强度，以便更好地观察样本。

2. 正常情况下，光源应该从样本底部照射并通过样本透射到目镜中。

第六步：观察和记录1. 调节好焦距和放大倍数后，即可通过目镜观察样本。

2. 可以使用调焦轮进行微调，以获取更清晰的图像。

还可以通过调节光源以改变样本的亮度和对比度，以便更好地观察细节。

3. 可以使用显微镜上的调节旋钮来对样本进行聚焦，旋转显微镜的聚焦盘或调节螺丝，观察到不同焦点的图像。

4. 还可以使用显微镜上的目镜游标盘来测量或记录样本的尺寸。

第七步：注意事项1. 在使用显微镜之前，要确保工作区域干净整洁，并保护好显微镜以防止刮擦或污染。

2. 使用显微镜时，要轻柔地操作，避免碰撞和震动。

倒置金相显微镜操作规程一、介绍倒置金相显微镜是一种用于观察金相组织和微观结构的特殊显微镜。

本文将详细介绍倒置金相显微镜的操作规程，帮助读者正确地使用该仪器，准确地观察样品，并获取准确的分析结果。

二、操作步骤2.1 准备工作在使用倒置金相显微镜之前，需要进行一系列的准备工作，包括： 1. 清洁显微镜外壳，保持仪器的整洁。

2. 准备好所需要的显微镜玻璃片、草图纸、铅笔等实验工具。

3. 根据实验需要，选择适当的目镜和物镜。

2.2 样品处理1.将需要观察的样品制备成适当的尺寸和形状，并确保表面平整和清洁。

2.对于金相显微镜观察，通常需要对样品进行打磨和腐蚀处理，以显现出金属组织的结构和特征。

2.3 装置样品1.打开显微镜盖板，将准备好的样品放置在镜台上。

2.利用显微镜的调焦装置，将样品调至合适的焦距。

2.4 观察样品1.调节目镜的放大倍数，选择适当的放大倍数以便观察样品。

2.利用显微镜的聚焦装置，调节合适的焦点，使得样品的细节清晰可见。

3.使用显微镜的可调光源，调整光源的亮度和角度，以便更好地观察样品。

2.5 记录和分析1.使用草图纸和铅笔，将观察到的样品结构绘制下来，标注重要的特征。

2.根据所观察到的样品结构，进行分析和解释，并与相关的理论知识进行比较。

三、维护和保养3.1 每次使用后1.关闭显微镜的电源，将镜头调至最低位置，盖好显微镜盖板。

2.清理镜头和物镜，使用干净的纱布轻轻擦拭，避免使用硬物或化学溶液接触镜头表面。

3.2 定期维护1.保持显微镜的清洁和整洁，定期清理外壳和镜头。

2.定期校准显微镜的聚焦装置，确保焦距的准确性。

3.定期检查显微镜的光源，确保亮度和角度的稳定性。

4.如发现异常情况或故障，应及时联系维修人员进行维修和保养。

四、安全注意事项1.使用显微镜时，应注意保护眼睛和身体安全，避免直视强光源和对镜头施加过大的压力。

2.使用化学腐蚀剂时，应佩戴防护手套和护目镜，避免溅溶液伤及皮肤和眼睛。

Leica荧光倒置显微镜使用指南1、按下接线板电源，打开计算机屏幕电源和主机箱电源，双击桌面上Leica Application Suite图标进入程序。

2、打开显微镜光源，如需使用荧光灯，打开荧光灯光源，在记录本上记录下荧光灯使用时间。

3、显微镜物镜放大倍数有5倍、10倍、20倍、40倍，旋转物镜下转盘改变放大倍数。

4、明场使用方法：将透射光装置的聚光镜转盘旋转至BF档。

选择合适的物镜，调节焦距，用明视光源强度旋转及透射光装置上的光圈调节光源强度。

将滤色块放在第4或5档。

5、相差使用方法：将透射光装置的聚光镜转盘转至PH1档（物镜为10×、20×）或PH2档（物镜为40×时）。

用明视光源强度旋转及透射光装置上的光圈调节光源强度。

将滤色块放在第4或5档。

（5×物镜无相差功能）6、荧光使用方法：放下明场遮拦，拉开荧光遮拦棒，旋转滤色块转盘，选择合适的激发波长。

用荧光光源视野调节旋钮和荧光光源光圈调节旋钮调节荧光的范围和强度。

7、图像处理：在“摄取”菜单栏Micl卡片下根据所选的物镜倍数选择放大倍数。

拉开摄像头操纵杆，单击鼠标左键选择一块白色区域，单击鼠标右键，进行白平衡。

可以通过改变摄取菜单栏摄像头卡片下曝光，饱和，伽马和增益改变现实图像的效果。

明场或者相差模式，建议将增益放在零。

单击拍摄，新建自己的文件夹，保存图片。

8、荧光拍摄完毕，推进荧光遮拦棒，看一下显微镜使用登记表。

（1）如果下一个使用登记时间小于或等于1个小时，不需要关闭荧光灯光源，记录下荧光灯光源使用时间。

将明场电源旋至最低。

将物镜旋至空挡，关闭应用程序。

填写完整仪器使用记录。

（2）如果下一个使用登记时间大于1个小时，使用完毕荧光及时关闭荧光灯光源，注意需长于半个小时，记录下荧光灯光源使用时间。

将明场电源旋至最低，关闭明场电源。

将物镜旋至空挡，关闭应用程序，关闭电脑。

填写完整仪器使用记录。

倒置荧光显微镜使用注意事项1、使用显微镜前，请和仪器负责人联系。

Leica DM ILInverted Microscope –for all Routine and Laboratory Applications2Leica Design by Ernest Igl /Christophe ApothélozLeica DM IL – compact inverted microscope for laboratory routine The new inverted Leica microscope blends ergonomy, a compactdesign and effective contrasting methods into a system for virtuallyunlimited life science applications.The integration of Leica HCS* optics extends the range of objectivesfor inverted microscopy.For the first time, high-quality relief contrast can be producedwithout special objectives with our new Integrated ModulationContrast (IMC) technique.Optimised phase contrast and brilliant incident light fluorescencemake the Leica DM IL the number one choice in contrastingmicroscopes.3With its unbeatable modularity, ergonomy and free view of thespecimen, teamed with newly developed and optimised contrastingtechniques, the Leica DM IL offers you a top level introduction toinverted microscopy.The adaption of the Leica DM IL to infinity optics allows theintegration of Leica HCS* components for superb image resolution,brilliant contrast and precise colour rendering. The DM IL is theinverted equivalent of our successful upright DM microscopes ofthe L and R class.The Leica DMIL is a microscope for all applications in microbiologyor the cell culture laboratory. A universal inverted microscope forroutine use: stable and space-saving, flexible and upgradablewith optics from Leica’s research microscopes.* Harmonic Component System Microinjection of oocytes in mouseRNA microinjection of frog oocytes(Xenopus)4The stand The stand of the Leica DM IL microscope is stable, aluminium-cast and excellently designed. There are two versions for biological applications:The DM IL for brightfield, phase and modulation contrast and the DM IL with additional incident light fluorescence unit.Appreciated by users for many years, the stable T-shaped micro-scope base offers plenty of valuable space round the microscope and ensures comfortable, fatigue-free microscopy. The micro-scope’s footprint is optimised to provide the necessary room for experiments, and all controls are ergonomically located. Many different components can be adapted to suit individual require-ments. The high stability, low centre of gravity and four vibration damping feet eliminate vibrations even in extreme conditions. The excellent stability of the DM IL also makes it the ideal solution for photography with long exposure times. In addition, finite element calculations and thorough practical testing in a wide variety of applications guarantee focusing which is not only ultra precise but also stable over long periods of time.The Leica DMIL contrasting microscopeThe System Discussion unitCardiac muscle cells5Due to its modularity, the Leica DM IL is particularly suitable forliving cell microscopy.Its modern, practical design, the integration of state-of-the-art,top quality optics and the excellent standard of the adaptedcontrasting techniques prove useful in research tasks as well asroutine applications.You will be convinced by the DM IL’s first-class technology andmany innovative and practical ideas.We at Leica believe microscopy should be a pleasurable experienceand designed the DM IL to be associated with enjoyment at thelaboratory workplace.Trinocular tube with DC 100Ergonomic phototubeTrinocular tube with MPS DM IL with illumination column the other way round6Nosepiece focusingSamples are focused with the quadruple objective nosepiece.The reliability, stability and precision of the focusing is not influenced by the microscope stage and the samples on it or by accessory components such as the object guide and manipulators.Illumination system The compact brightfield illumination unit is attached to a column and can be comfortably adjusted. Setting the correct height for the condenser used or the specimen on the stage is facilitated by markings along the column. The pre-centred, extremely powerful 6V 35W halogen lamp provides optimal illumination even of critical specimens. The transmitted light illumination concept is rounded off by the integration of the contrast slide (for phase and modulation contrast), the module for light filters with 32 mm diameter and the aperture diaphragm. The microscope stage with illumination arm can be turned round by 180°and is freely accessable for sample positioning from three sides.Light filters The filter module on the illumination column accommodates 32 mm diameter filters in a spoon-shaped holder. We are constantly adding to our wide range of filters, enabling you to selectively optimise the illumination for observation and image documentation.Built-in 6V 35W power supply The 6V 35W power supply, which provides the full lamp power including on/off switch, status indicator and brightness adjustment,is fully integrated in the microscope stand. Apart from the ergonomic advantages, this saves space on the microscope desk and allows the microscope to be easily picked up as a unit and moved elsewhere.The Technology7CondensersThe Leica DM IL offers you a choice of two condensers.The S 90/0.23 condenser with a free working distance of 90 mm and a numerical aperture of 0.23 is designed for brightfield and phase contrast and is particularly suitable for specimens in bulky laboratory vessels.The S 55/0.35 condenser with a free working distance of 55 mm and a numerical aperture of 0.35 for bulky containers is designed for brightfield, phase and integrated modulation contrast (IMC)and is particularly suitable for higher magnifications or thick specimens.Without a condenser, the maximum free working distance is 200 mm.Stage and accessoriesThe DM IL offers a wide variety of stages with a whole array of accessories and different inserts for your specimen vessels:The standard stage is a fixed stage plate of 252 x 212 mm. The stage can be widened by 70 mm on both sides by adapter plates. The interchangeable stage inserts (20-50 mm) allow smaller petri dishes to be used as well without losing the focus when the objective nosepiece is rotated.Object guides can be attached to both the left and right of the stage and have a minimum adjustment range of 83 x 127 mm. The control of the coaxial drive is in an ergonomically low position so that you can rest your hands on the desk while scanning specimens.The object guides accommodate special and multi-purpose frames for all types of culture vessels.A heating stage up to max. 45°C, a 3-plate mechanical stage and scanning stages round off the range of stages for the DM IL.Micromanipulation ...... and scanning stage8DM LB tubeEyepieces and objectivesThe optics are the heart of every microscope and decisive for the quality of information. We have set new standards here by intro-ducing our HC optics.The Leica DM IL is designed for brightfield, phase contrast, Inte-grated Modulation Contrast (IMC) and incident light fluorescence.All infinity-corrected high performance objectives in the Leica range with 25 mm screw thread are compatible with the DM IL microscope.Even earlier-type Leica objectives can be adapted for use on the DM IL. We offer a wide range of special objectives for inverted microscopy applications with long free working distances (L objectives) and/or with correction mounts (Corr objectives) to compensate for different vessel thicknesses. The latest Leica optics brochure features our whole range of objectives.Depending on the tube configuration, there is a wide choice of eyepieces with magnifications 10x, 12.5x, 15x, 16x or 25x, suitable for fields of view up to 20 mm. Besides special high-point eyepieces for eyeglass wearers, we also supply eyepieces with adjustable eyelens (M eyepieces), into which different types of graticule can be inserted.The DM IL range also comprises many different observation and photo tubes. The tubes are interchangeable and can be individually rotated by 360°in the tube mount and then fixed in position. All tubes are fitted with an infinity tube lens 1x. The following tubes are used on the DM IL:•Binocular tube ILB, with 45°viewing angle, for eyepieces with 23.2 mm outer diameter •Trinocular (photo)tube ILT, with 45°viewing angle, for eyepieces with 23.2 mm outer diameter, with vertical photo/TV exit with switchable light path for either 100% visual or 100% photo/TV.The position of the photo/TV exit 88 mm to the side of the tube has the special advantage that it allows an unobstructed view of the specimen.Other tubes from the Leica DM L range can be used via an IL/L adapter:•Binocular tube HC LB 45°viewing angle •Binocular tube HC LVB 0-35°ergotube •Trinocular (photo)tube HC L1T 45°viewing angle •Trinocular (photo)tube HC L3T 45°viewing angle •Trinocular (photo)tube HC LV1T 0-35°ergotubeThe Optics351+364 mn UVAr 488mn 568mn 647mn Ar/Kr 4905205575766486703504004505005506006507009Developments in diagnostics and med./biol. research (e.g. fluores-cence applications) and the increasing use of video technology and electronic image processing have to be paralleled by intelligent technical adaptations of the microscope system.The new HCS optics concept introduced with the Leica DM R microscope meets this requirement. It is the result of an integral system consideration, harnessing all technological potential.The abbreviation HCS stands for Harmonic Component System.Its special features are:•well-balanced optical and mechanical fitting dimensions•harmonious balance of all optical system components, i.e. the parameters contributing to the microscope’s performance (objectives, tube lenses, tubes, eyepieces, TV cameras/adapters,etc.) have been harmonised throughout the entire optic system.This has created scope for even greater optical opportunities.The HCS system is the answer to your application requirements not only today, but in future, too.Rat testicles, IMC10The Leica DM IL is the microscope for all requirements in the cell culture lab. A universal inverted microscope for routine application: stable and space-saving, flexible and upgradable with optics from Leica research microscopes.BrightfieldThe whole range of objectives from 4x-100x magnification can be used for brightfield applications. Samples in almost any kind of vessel can be examined with or without a condenser, while a 6V 35W halogen lamp ensures optimal illumination.Phase contrastIn vivo/ in vitro microscopy specimens are mostly living cultures or microorganisms and are examined under sterile conditions. Contrast of the transparent tissue can only be enhanced by optical methods.Phase contrast is a useful technique for high-contrast imaging of unstained specimens. The phase contrast technique used by Leica on the DM IL has been optimised for inverted microscopy applications and produces equally excellent contrast in watery solutions and of dry preparations in petri dishes.Spinal cord, catContrastingLymphocyte toxicity testdouble staining, strongly positiveLymphocyte toxicity testdouble staining, weakly positive11Human brain Femtotip microinjection needle (Photo: Eppendorf)FibroblastsIMC (Integrated Modulation Contrast)The innovative technique of Integrated Modulation Contrast (IMC)now introduced by Leica in the DM IL is based on Hoffman’sprinciple and produces this contrast without the need for specialobjectives–ordinary brightfield or phase contrast objectives canbe used. The Leica IMC provides a high-contrast, 3D image oftransparent objects similar to that of interference contrast. Plasticculture vessels do not impair the quality of the image as thetechnique is polarisation-neutral.The diaphragm slide on the side of the illumination and theswitchable modulator in the intermediate image of the pupilproduce the type of contrast named after Hoffman without modi-fying the objectives. High contrast, high resolution, a halo-freerelief image of either stained or unstained specimens make Leica’sIMC a new standard in the class of inverted routine microscopy.FluorescenceThe fluorescence model of the DM IL reflects the growing signifi-cance of fluorescence for in vivo/in vitro microscopy. The maincomponents of this configuration are an incident light axis integrat-ed in the microscope stand, incorporating a fluorescence slide forthree filter cubes. A wide range of light sources with multi-lens,chromatically corrected collectors brighten up even the weakestfluorescence. The fluorescence filter cubes comprise an optimallymatched combination of excitation, reflection, band-pass andbarrier filters. We are constantly updating our range of filtercubes to keep pace with the latest challenges in biology andmedicine.Transmitted light techniques can be used simultaneously or inalternation in order to clearly allocate fluorescent and non-fluorescent structures.The Leica DM IL offers you a powerful system for immunology,cytopathology, virology – in fact wherever fluorescence techniquesare used in combination with inverted microscopy.Leica Microsystems – the brand for outstanding products Microscopes Compound Stereo Surgical Laser Scanning Photomicrography Video Microscopy Measuring Microscopes Advanced Systems Image Analysis Spectral Photometry Automated Inspection Stations Measurement Systems Electron Beam Lithography Laboratory equipment Tissue Processors Embedding Systems Routine- & Immunostaining Coverslippers Refractometers Microtomes Sliding, Rotary & Disc Cryostats Ultramicrotomes EM Sample Preparation Leica Microsystems’ Mission is to be the world’s first-choice provider of innovative solutions to our customers’ needs for vision, measurement, lithography and analysis of microstructures.Leica, the leading brand for microscopes and scientific instruments, has developed from five brand names, all with a long tradition: Wild, Leitz, Reichert, Jung and Cambridge Instruments. 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倒置生物显微镜DMI3000B简要操作说明
1.接通电源，通过“开/关”接通显微镜电源。

2.根据使用者的眼间距调节双目镜筒的间距。

3.观察方法：
（1）亮视野观察
A.把聚光器设置到亮视野位置（在0-8范围内调到适当的位置）。

B.插入一个样品在载物台上。

C.先选择低倍物镜，通过右侧的转动杆调整载物台的位置，找到合适的视野。

D.用左侧下方的光线分压器调节亮度。

E.利用调焦操纵轮聚焦图像（左手侧聚焦操纵轮能用于粗略调焦，也可用于
精确调焦；右手侧聚焦操纵轮只能用于精确调焦）。

F.再转换成合适倍数的物镜聚焦进行观察。

（2）相衬观察（推荐用于活体细胞观察）
A.完全敞开聚光器的光圈（调节到ph位置）。

B.插入需要观察的样本。

C.选择一个相衬物镜（标有PH0/PH1/PH2），先用低倍物镜找视野。

D.在聚光器上选择与物镜对应的光圈（选物镜上标有PH0，则要将光圈转盘
转到0位置；标有PH1，则要转到1位置；标有PH2，则要转到2位置）。

E.用左侧下方的光线分压器调节亮度。

F.利用调焦操纵轮聚焦图像。

G.再换成合适倍数的物镜聚焦进行观察。

【注意事项】
1.显微镜系精密仪器，所有切换，动作要轻，要到位，务必仔细耐心使用。

2.如镜头上有指纹或污迹用擦镜纸轻轻将其擦除。

打扫卫生。

Leica荧光倒置显微镜操作步骤△显微镜系精密仪器，务请不要碰击，要仔细耐心使用。

△荧光光源关闭后，15分钟内不得再开启。

△荧光灯泡的最佳使用期限是200小时，因此每次使用时请尽量缩短时间。

⚫普通光源操作1. 插上电源插座，打开主机右下侧电源开关。

2. 将待检标本置于载物台上。

3. 用10×物镜对焦点，根据使用者的眼间距调节双目镜筒的间距。

4. 调节光圈及灯光强度至合适位置.5.调节粗调和微调使标本至最清晰。

6.拔出活动杆，使光路通过CCD至显示器。

7.保存图象至电脑中。

8.使用完毕后关掉电源，如镜头上有指纹或污迹用擦镜纸将其擦除。

⚫荧光光源操作1.插上荧光光源电源插座，打开荧光光源电源开关。

2.打开光栅。

3.根据使用荧光素的不同选择不同的荧光滤光片。

4.将待检标本置于载物台上。

5.用10×物镜对焦点，根据使用者的眼间距调节双目镜筒的间距。

6.调节粗调和微调使标本至最清晰。

7.拔出活动杆，使光路通过CCD至显示器。

8. 保存图象至电脑中。

9. 使用完毕后关掉电源，如镜头上有指纹或污迹用擦镜纸将其擦除。

10. 登记使用情况。

Leica DM主要技术参数：放大倍数10-400倍，内置滤色镜，数字成像头。

倒置明场、相差、荧光/明场、荧光/暗场。

波长：330-380，450-490，510-560。

主电90~250V。

频率：50~60HZ。

功率消耗：最大160W。

周围环境温度：10~36℃。

相对湿度：0~80%。

污染级别：2。

应用范围：倒置明场、相差及荧光，主要用于细胞及细菌等的观察及荧光摄影。