2016年6月 CIESC Journal ·2410· June 2016第67卷 第6期 化 工 学 报 V ol.67 No.6
DOI ：10.11949/j.issn.0438-1157.20160176
溶菌酶分子印迹聚乳酸微球的制备及吸附性能
唐志民，马新宾
（天津大学化工学院，天津 300072）
摘要：以改性聚乳酸微球（MPLA ）为载体，以γ-(2, 3-环氧丙氧)丙基三甲氧基硅烷为功能单体，以四乙氧基硅烷
为交联剂，采用溶胶凝胶方法制备了表面溶菌酶分子印迹改性聚乳酸微球 (LZY-MIP-MPLA)，采用红外、扫描电
镜和粒径测定等方法对LZY-MIP-MPLA 进行了表征，优化了制备条件。详细研究了LZY-MIP-MPLA 对溶菌酶的
吸附性能，考察了pH 和NaCl 对吸附性能的影响。结果显示，LZY-MIP-MPLA 对溶菌酶的吸附能力明显大于非
印迹改性聚乳酸微球（NIP-MPLA ），达到吸附平衡的时间为200 min 左右。Scatchard 方程的分析表明，印迹孔穴
对模板分子的作用是不完全等价的，即存在两类不同的结合位点。LZY-MIP-MPLA 对溶菌酶和牛血清白蛋白的分
离因子为10.13，说明其对溶菌酶具有较好的选择吸附性能。
关键词：改性聚乳酸；溶菌酶；分子印迹；酶；吸附；选择性
中图分类号：R 927.2
文献标志码：A 文章编号：0438—1157（2016）06—2410—07
Preparation and adsorption properties of lysozyme molecularly imprinted
polylactide microsphere
TANG Zhimin, MA Xinbin
(School of Chemical Engineering and Technology , Tianjin University , Tianjin 300072, China )
Abstract: Lysozyme molecularly imprinted modified polylactide microsphere (LZY-MIP-MPLA) was prepared by sol-gel using modified polylactide microsphere as carrier, 2,3-epoxypropoxy propyltrimethoxysilicane as the functional monomer and tetraethyl orthosilicate as crosslinker. The prepared material was characterized by infrared spectroscopy and scanning electron microscopy. The preparation conditions were optimized. The adsorption properties of LZY-MIP-MPLA on lysozyme were studied in detail, and the influence of pH and NaCl on adsorption property was also investigated. The results showed that the adsorption capacity of LZY-MIP-MPLA on lysozyme was better than that of NIP-MPLA, and it needed 200 min to reach the equilibrium. By the analysis of Scatchard equation, the interactions of imprinted cavities and template molecule were not exactly equivalent and there existed two kinds of interaction places. The separation factor of LZY-MIP-MPLA on lysozyme and bovine albumin was 10.13, showing that LZY-MIP-MPLA displayed high recognition ability to the template molecule LZY.
Key words: modified polylactide; lysozyme; molecularly imprinted; enzyme; adsorption; selectivity
引 言 表面分子印迹技术是将分子印迹聚合物以某
种方式结合于载体的表面，使分子印迹识别位点位于颗粒的表面(或表层), 目标分子更易接近印迹点，
有利于印迹分子的结合与洗脱，可以避免传统分子 2016-02-17收到初稿，2016-03-10收到修改稿。
联系人：马新宾。第一作者：唐志民（1970—），男，博士研究生，高级工程师。 Received date : 2016-02-17. Corresponding author : Prof. MA Xinbin, xbma@https://www.doczj.com/doc/d018311969.html,