Soil biological activity and their seasonal variations in response to long-term application

  • 格式:pdf
  • 大小:210.20 KB
  • 文档页数:14

REGULARARTICLE

Soilbiologicalactivityandtheirseasonalvariations

inresponsetolong-termapplicationoforganic

andinorganicfertilizers

GaofeiGe&ZhaojunLi&FenliangFan&GuixinChu&ZhenanHou&YongchaoLiang

Received:13July2009/Accepted:29September2009/Publishedonline:9October2009#SpringerScience+BusinessMediaB.V.2009

AbstractTheobjectivesofthisstudyweretoexplore

theeffectsoflong-termandcontinuedapplicationof

fertilizersandmanuresonmicrobialbiomass,soil

biologicalactivityandtheirseasonalvariationsin

surfaceandsubsurfacesoilsinrelationtosoilfertility.

Forthis,soilsweresampledinspring,summerand

autumnfromShenyangLong-termExperimental

Station,northeasternChina.Theresultsshowedthat

soiltotalnitrogen(N),organiccarbon(C),basal

respiration,microbialbiomassandenzymaticactivity

increasedinmanure-amendedsurfacesoils,but

decreasedwithsoildepth.Long-termapplicationofinorganicfertilizerssignificantlydecreasedsoilpH

value,sucraseactivityandmicrobialbiomassC,but

increasedsoilmetabolicquotient(qCO2).However,

nosignificanteffectofinorganicfertilizersonsoil

totalN,ureaseactivityandmicrobialbiomassNwas

observedincomparisonwithCK0(neithertillagenor

fertilization)andCK(nofertilizers).Therewasno

significantdifferencebetweenCK0andCKinsoil

totalN,organicCandmicrobialactivityinsurface

soillayer(0–20cm),buttheseparametersin

subsurfacesoillayer(20–40cm)werehigherinCK

thaninCK0.Moreover,seasonalchangeswere

observedintermsofsoilnutrientcontents,enzymatic

activity,microbialbiomassandsoilrespiration.There

weresignificantcorrelationsbetweensoilmicrobial

biomassCandN,betweenorganicCandsucrase

activityandbetweentotalNandureaseactivity,

respectively.Itisrecommendedthatcombineduseof

organicmanurewithinorganicfertilizersshouldbe

consideredtomaintainhighermicrobialbiomass,soil

biologicalactivityandsoilfertility.Considering

considerablyhighnutrientsreserveandmicrobial

activityinsubsurfacelayersofsoilandwind-

erosion-causednutrientlossinspringinnorthChina,

wealsoproposethatlowtillageshouldbeconsidered

tomakeuseofnutrientsinsoils.

KeywordsEnzymaticactivity.Microbialbiomass.

OrganicC.Soilfertility.SoilrespirationPlantSoil(2010)326:31–44DOI10.1007/s11104-009-0186-8

ResponsibleEditor:YongguanZhu.

G.Ge:Z.Li:F.Fan:Y.LiangMinistryofAgricultureKeyLaboratoryofCropNutritionandFertilization,InstituteofAgriculturalResourcesandRegionalPlanning,ChineseAcademyofAgriculturalSciences,Beijing100081,People’sRepublicofChina

G.GeCollegeofResourcesandEnvironmentalSciences,NanjingAgriculturalUniversity,Nanjing210095,People’sRepublicofChina

G.Chu:Z.Hou:Y.Liang(*)KeyLaboratoryofOasisEco-Agriculture,CollegeofAgriculture,ShiheziUniversity,Shihezi,Xinjiang832003,People’sRepublicofChinae-mail:ycliang@caas.ac.cnIntroduction

Soilbiologicalactivitymainlyincludingsoilenzymatic

activity,basalrespirationandmicrobialbiomasshasbeen

showntobecloselyrelatedtobothvarioussoilfactors

includingpHvalues,soilorganicmatter,soiltexture,and

modifyingfactorssuchasclimate,soilmoistureandsoil

temperatureregimes(Emmerlingetal.2001;Bastidaet

al.2008).Microbialbiomass,respiration,metabolic

quotient(qCO2,anindexoftherespirationactivityper

biomassunit,CO2-C/MBC)andsoilenzymaticactivi-

tiescanbeutilizedasindicatorsforchangesinsoil

quality(WardleandGhani1995;Rosetal.2003;

Bastidaetal.2008).Enzymesaremainlyfromsoil

microorganisms,anddespitetheirrelativelylow

amounts,playacrucialroleinkeepingnutrientcycling

insoilssuchasC,N,P,andS.Nutrientcyclingis

fundamentalnotonlyforprimaryproductionbutforthe

long-termfunctioningofecosystemsaswell(Doranand

Parkin1996;Aonetal.2001).Inaddition,soil

microbialactivitiesincludingsoilrespiration,microbial

biomass,andsoilATPcontenthavebeenprovedtobe

powerfulindicatorsofsoilquality(Bendingetal.

2004;Gobernaetal.2006;Bastidaetal.2008).Soil

microbialactivityhas,forexample,beenfoundtobea

sensitiveindicatorofsoilagriculturalpractices,input

offertilizers,organicresiduesandcroprotation

(Emmerlingetal.2001;Bendingetal.2004).Soil

respiration,ameasureofsoilmicrobialactivity,is

crucialtoestimatingbiologicalprocessinsoilecosys-

tems(IslanandWeil2000).

Theapplicationofbalancedamountoffertilizersand

manurescouldincreaseenzymaticactivityandsoil

respiration(KanchikerimathandSingh2001;Tuetal.

2006).Organicfertilizersusuallyincreasesoilmicro-

bialbiomass(Kauretal.2005;Liangetal.2003,

2005),CO2evolution,andenzymeactivities(Crecchio

etal.2001;Liangetal.2003).Organicmanure-

stimulatedbiologicalactivityandsoilmicrobialbio-

masshasbeenproventobepositivelycorrelatedwith

improvedsoilfertilityandqualityasindicatedby

highercropbiomassandhigherconcentrationofsoil

availablenutrientsandplantnutrientuptake(Muelleret

al.1998;Bendingetal.2004;Liangetal.2003,2005;

Tuetal.2006).Furthermore,thesebiologicalparam-

eterscanbeusedassensitivebio-indicatorsofsoil