Supercritical carbon dioxide-treated blood orange juice as a new product in the fresh fruit juice ma
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Supercriticalcarbondioxide-treatedbloodorangejuiceasanewproductinthefreshfruitjuicemarket
SimonaFabroni,MargheritaAmenta,NicolinaTimpanaro,PaoloRapisarda⁎CRA—CentrodiRicercaperl'AgrumicolturaeleColtureMediterranee,CorsoSavoia190,95024Acireale(CT),Italy
abstractarticleinfoArticlehistory:Received28December2009Accepted28February2010
EditorProofReceiveDate30March2010
Keywords:ContinuousprocessFreshbloodorangejuiceHigh-pressureCO2
Antioxidants
MicrobialsurvivalSensoryattributes
Theworkdescribedheredealswiththeeffectivenessofusinghigh-pressurecarbondioxidetreatment(HPCD)tostabilisefreshlysqueezedbloodorangejuice.Technicalplanningofacontinuoushigh-pressuresupercriticalcarbondioxidepilotsystem,suitablefordevelopmentonanindustrialscale,wascarriedoutinourlab.Todeterminetheoptimaloperatingconditions(temperature,pressure,andCO2/juiceratio),three
differentexperimentaltrialswerecarriedout.Thefirsttrialwasconductedat230bar,36±1°C,5.08L/hjuiceflowrate,and3.91L/hCO2flowrate,correspondingtoagCO2/gjuiceratioof0.770.Thesecondtrial
utilisedthesameconditionsexceptthattheoperativepressurewasreduced(130bar).Thethirdtrialwascarriedoutat130bar,36±1°C,5.08L/hjuiceflowrate,1.96L/hCO2
flowrate,correspondingtoa
0.385gCO2/gjuiceratio.Theeffectsofprocessingwereevaluatedbydeterminingphysicochemical,antioxidant,
andmicrobiologicalparametersofthetreatedjuices.Inaddition,oncethebestoperativeparametershadbeendetermined,physicochemical,antioxidant,microbiologicalandsensoryevaluationoffreshbloodorangejuicestabilisedbyHPCDtreatmentwascarriedoutduringrefrigeratedstorageofjuicesat4±1°Cforthirtydays.TheresultsshowedthatHPCDtreatmentcannotbeconsideredasanalternativetotraditionalthermalmethodsbutasanewmildtechnologyforproducingastabilisedbloodorangejuicewithashelf-lifeof20days.Industrialrelevance:BloodorangesarethemaincultivatedvarietiesofCitrussinensis(L.)OsbeckinItaly.Freshlysqueezedbloodorangejuiceexertahighantiradicalandantioxidantactivity,duetoitsrichphenolicprofile,butitspreservationisusuallyassuredbythermaltreatmentwhichaffectsitsnutritionalandsensoryvalue.Inthisstudyweproposeda“milder”continuousHPCDprocesssuitableforimplementationonanindustrialscale.TheHPCDstabilisedjuiceretainsitsphysicochemical,antioxidant,andsensorypropertiesandcouldbeplacedwithinanewretailframework,namely,thatoffreshjuiceswithashelf-lifeof20days.©2010ElsevierLtd.Allrightsreserved.
1.IntroductionThermalfoodpreservationisanestablishedandwell-knowntechniqueusedtostabilisefoods.Althoughpasteurisationisveryeffectiveforthemicrobiologicalstabilisationoffoodsandinactivatingpectinmethylesterase(PME)infruitjuices(Marcotte,Stewart,&Fustier,1998;Shomer,Cogan,&Mannheim,1994),thistreatmentaffectstheintrinsicqualityofamajorityoffoodproductswithregardstotheirnutritionalandorganolepticvalue,especiallyforfruitjuices(Shomeretal.,1994).Duetotheincreaseinconsumerdemandfornutritious,fresh-likefoodproductswithahighorganolepticqualityandanextendedshelf-life,non-thermalprocessingalternativeshavebeenproposed.Amongthesenon-thermalinactivationtechnologies,highhydrostaticpressure(HHP)andpulsedelectricfields(PEF)arethemostinvestigatedones(Devlieghere,Vermeiren,&Debevere,
2004;Jeyamkondan,Jayas,&Holley,1999).AlthoughHHPoffersgreatopportunitiesforfoodpreservation,italsoentailsalargeinvestmentofcapitalduetothehighpressuresinvolved.EffortstousethePEFmethodonacommercialscaleforthepasteurisationoffoodhaveresultedinatleasttwoindustrial-scalePEFsystems,includingtreatmentchambersandpowersupplyequipment(Butz&Tauscher,2002).However,PEFtechnologyhasnotyetattainedthecommercialusabilitystage(Gerlachetal.,2008),whilethehighcapitalcostofsuchsystemsremainsamajorobstacletoitsindustrialapplication.Theuseofhigh-pressurecarbondioxide(HPCD)hasbeenproposedasanalternativenon-thermalpasteurisationtechniqueforfoods(Spilimbergo,Elvassore,&Bertucco,2003;Kincal,Hill,Balaban,Marshall,&Wei,2005;Boff,Truong,Min,&Shellhammer,2003).ManyconflictinghypothesesregardingmicrobialinactivationbyHPCDcanbefoundintheliterature(Nakamura,Enomoto,Fukushima,Nagai,&Hakoda,1994;Enomoto,Nakamura,Nagai,Hashimoto,&Hakoda,1997;Dillow,Denghani,Hrkack,Foster,&Langer,1999).However,theexactinactivationmechanismsremainunknown.Recently,Garcia-Gonzalezetal.(2007)proposedamodelforhow
InnovativeFoodScienceandEmergingTechnologies11(2010)477–484⁎Correspondingauthor.Tel.:+390957653134;fax:+390957653113.E-mailaddress:paolo.rapisarda@entecra.it(P.Rapisarda).
1466-8564/$–seefrontmatter©2010ElsevierLtd.Allrightsreserved.doi:10.1016/j.ifset.2010.02.004