检测胃癌淋巴结微转移的临床意义

主要缩略词简表英文缩写 英文全称中文全称 BCR B cell rceptor B 细胞受体CTL cytotoxic T lymphocytes细胞毒性T 细胞 DAB diaminobenzidin二氨基联苯胺 EBERsEBV-encoded small RNAs EB 病毒编码的小RNAs EBV Epstein-Barrvirus EB 病毒 EBVaGC EBV-associatedgastric carcinoma EB 病毒相关胃癌 HE haematoxylineosin 苏木素-伊红 HSPheat shock protein 热休克蛋白 ITAMimmunoreceptor tyrosine-based activation motif 免疫受体酪氨酸活化基序JNK c-Junc-NH2-terminalkinase c-Junc 氨基末端激酶 LC3Microtubule-associated protein 1 light chain 3 微管相关蛋白1轻链3 LMP1 Latentmembrane protein-1 潜伏性膜蛋白1 LMP2A Latentmembrane protein-2A 潜伏性膜蛋白2A NPCNasopharyngeal Carcinoma 鼻咽癌 PBSphosphate buffer solution 磷酸盐缓冲液 PCRpolymerase chain reaction 聚合酶链反应 PRSS1protease serine 1 丝氨酸蛋白酶1 PI3-K phosphotidylinositol3-kinase 磷酸酞肌醇3激酶 PTKs ProteinTyrosine Kinases 酪氨酸蛋白激酶 SPstreptavidin-peroxdase 链霉菌抗生物素 蛋白-过氧化物酶 STATThe signal transducer and activator of transcription 转录活化因子胃癌组织中LMP1、LC3、PRSS1及HSP90α/β表达分析及临床意义摘要:目的:观察EB病毒LMP1、PRSS1、HSP90α/β与自噬蛋白LC3在胃癌组织中的表达情况，探讨EB病毒LMP1在胃癌发生发展的作用与意义。

胃癌中BDNF的表达特征及其临床意义窦维佳;王景杰;刘震雄【摘要】目的检测脑源性神经营养因子(brain-derived neurotrophic factor,BDNF)在胃癌、癌旁以及转移淋巴结组织中的表达,探讨BDNF与胃癌组织临床病理特征的关系,并初步评价其意义. 方法收集72例胃癌组织、相应癌旁组织和34例转移淋巴结组织,采用免疫组织化学方法检测各组织中BDNF的表达,并进一步分析其表达与胃癌临床病理特征之间的关系;用受试者工作特征曲线(receiver operating characteristic curve,ROC)评估BDNF表达对胃癌诊断的价值. 结果与癌旁组织相比,BDNF在胃癌中的表达显著上调(P＜0.01),转移淋巴结组织中BDNF 的表达水平明显高于胃癌(P＜0.05)及相应癌旁组织(P＜0.01).BDNF表达与肿瘤分化程度(P＜0.05)、TNM分期(P＜0.05)、淋巴结转移(P＜0.01)及远处转移(P＜0.05)密切相关.ROC曲线显示BDNF表达对胃癌具有一定的诊断价值(95％ CI 0.645-0.811,P＜0.001). 结论 BNDF的高表达与胃癌的不良临床病理特征密切相关,可能在胃癌的发生发展中发挥癌基因功能,其可作为反映胃癌恶性生物学行为的重要指标,还能为胃癌诊断提供新的靶标.%Objective To investigate the expression of brain-derived neurotrophic factor(BDNF) in gastric cancer tissues,adjacent non-tumorous tissues,and metastatic lymph node tissues,and to explore the clinical significance of BDNF in gastric cancer.Methods Immunohistochemistry was performed to detect the expression level of BDNF protein in 72 gastric cancer tissues and paired adjacent non-tumorous tissues,and 34 metastatic lymph node tissues.The relationship between BNDF expression and clinicopathological parameters in gastric cancer was further analyzed.In addition,receiver operatingcharacteristic (ROC) curve was used to assess the diagnostic value of BDNF expression in gastric cancer.Results The expression of BDNF was significantly upregulated in gastric cancer compared with the corresponding non-tumor tissues (P ＜ 0.01).The expression level of BDNF in metastatic lymph node tissues was significantly higher than that of gastric cancer(P ＜ 0.05) and non-tumorous samples (P ＜ 0.01).The overexpressed BDNF was related with tumor differentiation (P ＜0.05),TNM stage (P ＜ 0.05),lymph node metastasis (P ＜ 0.01),and metastasis to other organs (P ＜ 0.05).ROC curve showed that the expression level of BDNF had a certain diagnostic value for gastric cancer(95％ CI 0.645-0.811,P ＜ 0.001).Conclusion Overexpression of BDNF is significantly correlated with poor clinical features,indicating BDNF might play an important role in the development of gastric cancer as an oncogene and be an important indicator in malignant biological behaviors of and a new target for the diagnosis of gastric cancer.【期刊名称】《山西医科大学学报》【年(卷),期】2018(049)004【总页数】4页(P383-386)【关键词】胃癌;BDNF;ROC曲线【作者】窦维佳;王景杰;刘震雄【作者单位】中国人民解放军空军军医大学(第四军医大学)唐都医院消化内科,西安710038;中国人民解放军空军军医大学(第四军医大学)唐都医院消化内科,西安710038;中国人民解放军空军军医大学(第四军医大学)唐都医院消化内科,西安710038【正文语种】中文【中图分类】R735.2尽管近年来发病率有所下降,胃癌仍是消化道最为常见的恶性肿瘤之一,是导致死亡的第二大恶性肿瘤,然而其发病机制尚不十分清楚[1]。