HAAM-PPAPLDL

The Hitachi HM62256A is a CMOS static RAM

organized 32-kword ×8-bit. It realizes higher

performance and low power consumption by

employing 0.8 µm Hi-CMOS process technology.

The device, packaged in a 8 ×14 mm TSOP with

thickness of 1.2 mm, 450-mil SOP (foot print pitch

width), 600-mil plastic DIP, or 300-mil plastic DIP,

is available for high density mounting. TSOP

package is suitable for cards, and reverse type

TSOP is also provided. It offers low power

standby power dissipation; therefore, it is suitable

for battery back up system.

Features

•High speed: Fast Access time 85/100/120/150 ns

(max)

•Low Power

Standby: 5 µW (typ) (L/L-SL version)

Operation: 40 mW (typ) (f = 1 MHz)

•Single 5 V supply

•Completely static memory

No clock or timing strobe required

•Equal access and cycle times

•Common data input and output: Three state

syringepumpsPumpssyringepumps

HarvardApparatus•phone508.893.8999•tollfreeU.S.800.272.2775•fax508.429.5732•PHD4400HpsiHighForce/HighPressureProgrammableSyringePump

A27ThePHD4400HpsiProgrammableSyringePumpisasinglesyringeInfusion/WithdrawalpumpequippedwithallthefunctionsofthePHD22/2000ProgrammableModel,butwithahigh-powersteppermotortoprovidemorethan200lbs.oflinearforce.ThePHD4400Hpsiisavailableintwoconfigurations;standardandremote.Theremotemodelissuppliedwithastandard5footcablethatconnectsthecontrolboxtothepumpingmechanism.PressureandSpeedThePHD4400Hpsicandeliverupto182.40ml/minusinga100mmstainlesssteelsyringe.Maximumpressureisdependentonsyringesize.Forstainlesssteelsyringes,seepageA74.FlexibilityandEasyProgrammability–Twostandardinfusionmodes(continuousorvolumedispense)–InProgramMode,complexinfusionandwithdrawalapplicationscanbeeasilycreated,storedinthepumpsnonvolatilememoryandrecalledforlateruse.–AutofillModeprovidescontinuousdeliverywhenthepumpisusedinconjunctionwitheitherastandardorhighpressurevalveboxandafluidreservoir,seepageA34.EaseofUseSettingthepumpisquickandeasy.InputtheinsidediameterofthesyringeorusetheinternalSyringeLookupTabletoautomaticallyinputthesyringediameterbasedonthesyringemanufacturerandsize.Selectamode(continuousdeliveryorvolumedispense)andarateandyouarereadytogo.Features–NonvolatileMemory:Storesalloperationaldata&programsequences.–StallDetection:Anopticaldetectorverifiesmotormovement.Stallsduetojammingorexcessivebackpressurearereported.–Visual/AudibleAlarms–Power-UpOptions:Powers-upinStandbyorRunningModeafterpowerinterruption–RS-232Connections:Allowscomputercontrolofsinglepumpordaisy-chainingofmultiplepumps.Alsoallowsforscaleandprinterconnections.–TTLConnections:Allowsforsynchronizingpumpwithexternaldevices,controllinganexternalvalve,changingdirectionoftravel,etc.ModesofOperation–Pump:Runscontinuouslyintheinfuseorrefilldirectionsuntilstopped.–Volume:Runsuntilspecifiedvolumehasbeenpumpedorrefilled.–Program:Pumpoperatesaccordingtospecifiedsequenceofinstructions.(Note:AllmodesinteractwithAutofillfeature.)KeyFeatures•Accurateandsmoothflow•Ideallysuitedforstainlesssteelsyringes•Easy-to-useinterface•RS-232serialportforcomputercontrol•Deliversover200lbsofpumpingforceacrossawideflowraterange•Legendaryreliability–2yearwarrantyApplications•HighPressureInjections•DrugDelivery•PumpingHighlyCorrosiveFluids•InjectingintoHighPressureReactionVesselsSpecificationsTypeMicroprocessorsinglesyringe,infusion/withdrawalAccuracy±0.35%Reproducibility±0.05%Syringe:TypeStainlesssteel**SizeMinimum2.5mlSizeMaximum100mlFlowRate:Minimum0.0076µl/minMaximum182.40ml/minNonvolatileMemoryStoresallsettingsRS-232RJ-114-conductorTTL9-pinD-subconnectorAverageLinearForce>200lbs(91kg)*FluidPressure*>1,800psiwithan8mlstainlesssteelsyringe,forexampleDriveMotor1.8°stepanglemotorMotorDriveControlMicroprocessorcontrolledfrom1/2to1/32microsteppingMotorStepsperOneRev.ofLeadscrew800stepsat1/2steppingor12,800at1/32steppingStepResolution0.082µm/stepStepRate:Minimum27.3sec/stepMaximum416.7µsec/stepPusherTravelRate:Minimum0.18µm/minMaximum190.676mm/minPower75W,0.75AfuseVoltageRangeUniversalinput100/240VAC,50/60HzDimensions,HxWxD17x23x29cm(6.7x9.0x11.4in)Weight6.4kg(14lbs)RemoteCable9.1m(30ft)Length*Forworkrange,refertoUser’sManualfordetails.**Plasticandglasssyringesarenotrecommendedbecauseofhighforce.PY270-2200PHD4400HpsiProgrammable

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中国医学科学院学报

ＡＣＴＡＡＣＡＤＥＭＩＡＥＭＥＤＩＣＩＮＡＥＳＩＮＩＣＡＥ

󰀡󰀢󰀣

󰀤󰀥󰀦󰀢

󰀧　２５７　

基金项目：国家自然科学基金（１１７０２０４７）、重庆市社会事业与民生保障科技创新专项（ｃｓｔｃ２０１７ｓｈｍｓＡ１３０００９）、重庆市医学科研计划项目（２０１７ＭＳＸＭ０７９）和

重庆市博士后科研流动站项目（Ｘｍ２０１７０８２）·论　著·

微流控芯片技术和流式细胞术检测替格瑞洛抗血小板疗效

黄小静，张天聪，高雪梅，何　翠，宦宣容，李　远

重庆医科大学附属永川医院中心实验室，重庆４０２１６０

通信作者：李　远　电话：０２３８５３８１７６０，电子邮件：ｌｉｙｕａｎ＿１９８５９９９＠１６３ｃｏｍ

摘要：

目的　应用微流控芯片技术和流式细胞术体外分析剪切力作用下替格瑞洛的抗血小板疗效。方法　采用微流

控芯片技术检测３００／ｓ、１５００／ｓ剪切率条件下替格瑞洛对血小板聚集行为的影响，以血小板聚集体表面覆盖率为量化指

标，计算替格瑞洛半抑制率；运用光学比浊法验证替格瑞洛抑制ＡＤＰ诱导的血小板聚集效应；应用微流控芯片构建体外

狭窄血管模型，探究高剪切率作用下血小板反应性，并采用流式细胞术分析替格瑞洛对高剪切诱导活化的血小板膜表面纤

维蛋白原受体（ＰＡＣ１）和Ｐ选择素（ＣＤ６２Ｐ）表达的影响。结果　在３００／ｓ、１５００／ｓ剪切率流动条件下，替格瑞洛呈浓

度依赖性抑制血小板聚集，３００／ｓ较１５００／ｓ抑制程度更明显（Ｐ均＜０００１），当浓度

≥４

μｍｏｌ／Ｌ时几乎完全抑制血小板聚

集；替格瑞洛抑制ＡＤＰ诱导的血小板聚集效应与流动条件下的结果相似，亦呈浓度依赖性抑制血小板聚集；替格瑞洛能

够抑制ＰＡＣ１和ＣＤ６２Ｐ的表达。结论　应用微流控芯片技术分析血小板聚集和流式细胞术检测血小板活化，与仅用ＡＤＰ

诱导的基于聚集的分析相比，可确定不同患者对替格瑞洛反应的差异性。

关键词：微流控芯片；流式细胞术；替格瑞洛；血小板聚集；剪切率；剪切诱导活化