矿区复垦土壤的微生物活性变化
- 格式:pdf
- 大小:639.64 KB
- 文档页数:6
矿区复垦土壤的微生物活性变化钱奎梅王丽萍李江中国矿业大学环境与测绘学院,江苏徐州221116摘要:在由煤矸石、粉煤灰和污泥构成的矿区复合基质上种植黑麦草进行矿区复垦试验,分析了不同复垦阶段土壤微生物功能多样性和酶活性的差异,以揭示矿区复垦过程中免施肥的可行性。
结果表明,矿区复垦土壤微生物功能多样性和酶活性随复垦时间的延长而显著提高。
复垦23个月后,矿区复垦土壤的全氮、速效磷和速效钾含量分别比复垦种植前降低72. 05%、67. 86%和60. 19%,蔗糖酶、脱氢酶、脲酶和碱性磷酸酶活性增加1.30~7.56倍。
Biolog微平板培养测试结果显示,矿区复垦16和23个月的土壤微生物群落AWCD( average well colour development)值,微生物Shannon多样性指数、Simpson指数、Mclntosh指数均显著高于复垦种植前(P<0.05)。
微生物多样性相关参数与土壤酶活性及土壤有机质含量之间呈显著正相关(P<0.05或P<0.01),与土壤全氮、速效磷、速效钾含量之间呈显著负相关(P<0. 05或P<0. 01)。
在矿区复垦过程中污泥作为有机添加物有助于促进植物生长和提高微生物活性。
矿区复垦土壤;Biolog;微生物群落功能多样性;酶活性X705;X172A 1673 -4831(2011)06-0059 -05Variation of Microbial Activity in Reclaimed Soil in Mining AreaQIAN Kui-meiWANG Li-ping LI Jiang2011-08-02基金项目:教育部重点实验室开放基金(CPEUKF06 - 04);江苏省省级环保科技项目(2007024)第27卷・61・第27卷@@[1]安丽,陈祖奇.污泥处置方法的研究[J].环境工程,2000,18 (1):40-43.@@[2]曹学章,刘庄,唐晓燕.美国露天采矿环境保护标准及其对我 国的借鉴意义[J].生态与农村环境学报,2006,22(4): 94-96.@@[ 3 ] WANG Li-ping, QIAN Kui-mei, HE Shi-long,et al. Fertilizing Rec lamation of Arbuscular Mycorrhizal Fungi on Coal Mine Complex Substrate[ J]. Procedia Earth and Planetary Seience,2009,1 ( 1 ) : 1101 - 1106.@@[4]毕银丽,吴王燕,刘银平.从枝菌根在煤矸石山土地复垦中的 应用[J].生态学报,2007,27(9):3738 -3743.@@[5] WANG Li-ping,ZHANG Wei-wei,GUO Guang-xia,et al. Selection Experiments for the Optimum Combination of AMF-Plant-Substrate for the Restoration of Coal Mines[J]. Mining Science and Technol ogy,2009,19(4) :479 -482.@@[6] CLAASSENS S,VAN RENSBURG P J J,MABOETA M S,et al. Soil Microbial Community Function and Structure in a Post-Mining Chronosequence[ J]. Water Air and Soil Pollution,2008,194( 1 ) : 315 -329.@@[ 7 ] GREEN J, BOHANNAN B J M. Spatial Scaling of Microbial Biodi versify[ J ]. Trends in Ecology & Evolution,2006,21 (9) :501 - 507.@@[8] HE Ji-zheng,GE Yuan,XU Zhi-hong,et al. Linking Soil Bacterial Diversity to Ecosystem Multifunctionality Using Backward-Elimina tion Boosted Trees Analysis [ J ]. Journal of Soils and Sediments, 2009,9(6) :547 -554.@@[9] 鲁如坤.土壤农业化学分析方法[M].北京:中国农业科技出 版社,1999:238 - 240.@@[10]关松荫.土壤酶及其研究方法[M].北京:中国农业出版社, 1996:274 -332.@@[11] GARLAND J L,MILLS A L. Classification and Characterization of Heterotrophic Microbial Communities on the Basis of Patterns of Community-Level Sole-Carbon-Source-Utilization[ J ]. Applied Environmental Microbiology, 1991,57 ( 8 ) :2351 - 2359.@@[12]黎炜,陈龙乾,周天建.张集矿区复垦土壤养分变化研究及评 价[J].现代矿业,2011(2):41 -43.@@[13]樊金拴,周心澄,王华.利用绿化技术对煤矸石废弃地进行生 态恢复与景观改造的尝试:以山东兖矿集团煤矸石山绿化为 例[J].中国园林,2006.22(3):57 -63.@@[14] STADDON W J,DUCHESNE L C,TREVORS J T. Microbial Di versity and Community Structure of Post Disturbance Forest Soils as Determined by Sole-Carbon-Source-Utilization[ J ]. Microbial Ecol ogy, 1997,34(2) :125 - 130.@@[15] DERRY A M,KEVAN P G,ROWLEY S D M. Soil Nutrients and Vegetation Characteristics of a Dorset/Thule Site in the Canadian Arctic[J]. Arctic, 1999,52(2) :204 -213.@@[ 16] GIL-SOTRES F,TRASAR-CEPEDA C,LEIR(O)S M C,et al. Differ ent Approaches to Evaluating Soil Quality Using Biochemical Prop erties [ J ]. Soil Biology and Biochemistry,2005,37 ( 5 ) : 877 - 887.@@[17]李影.王友保.蜈蚣草生长对其根际铜尾矿土壤酶活性的影响 [J].生态与农村环境学报,2011,27(2):75 -80.@@[18] YAN F, MCBRATNEY A B,COPELAND L. Functional Substrate Biodiversity of Cultivated and Uncultivated a Horizons of Vertisols in NW New South Wales[J]. Geoderma,2000,96(4) :321 -343.@@[ 19] ZHONG Wen-hui ,GU Ting,WANG Wei ,et al. The Effects of Min eral Fertilizer and Organic Manure on Soil Microbial Community and Diversity[J]. Plant and Soil,2010,326(1/2) :511 -522.@@[20] ALBIACH R,CANET R,POMARES F,et al. Microbial Biomass Content and Enzymatic Activities After the Application of Organic Amendments to a Horticultural Soil [ J ]. Bioresource Technology, 2000,75 ( 1 ) :43 - 48.@@[21 ] GOMEZ E,FERRERAS L,TORESANI S. Soil Bacterial Functional Diversity as Influenced by Organic Amendment Application [ J ]. Bioresource Technology,2006,97 ( 13 ) :1484 - 1489.@@[22] YANG Li-juan,LI Tian-lai,LI Fu-sheng,et al. Fertilization Regu lates Soil Enzymatic Activity and Fertility Dynamics in a Cucumber Field[ J ]. Scientia Horticulturae,2008 ,116( 1 ) :21 - 26.作者简介:钱奎梅(1982-),女,江苏连云港人,博士生,主要研究方向为矿区生态修复。
E-mail: ningjingmei@ 126. com矿区复垦土壤的微生物活性变化作者:钱奎梅, 王丽萍, 李江, QIAN Kui-mei, WANG Li-ping, LI Jiang 作者单位:中国矿业大学环境与测绘学院,江苏徐州,221116刊名:生态与农村环境学报英文刊名:Journal of Ecology and Rural Environment年,卷(期):2011,27(6)本文链接:/Periodical_ncsthj201106011.aspx。