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TAG-mapping PM resistance gene

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ORIGINALPAPERIdentificationandcomparativemappingofapowderymildewresistancegenederivedfromwildemmer(Triticumturgidumvar.dicoccoides)onchromosome2BS

ZijiLiu•JieZhu•YuCui•YongLiang•

HaibinWu•WeiSong•QingLiu•TsominYang•

QixinSun•ZhiyongLiu

Received:21June2011/Accepted:4December2011ÓSpringer-Verlag2011

AbstractPowderymildew,causedbyBlumeriagraminisf.sp.tritici,isanimportantfoliardiseaseofwheatworld-wide.Wildemmer(Triticumturgidumvar.dicoccoides)isavaluablegeneticresourceforimprovingdiseaseresistanceincommonwheat.Apowderymildewresistancegenecon-ferringresistancetoB.graminisf.sp.triticiisolateE09attheseedlingandadultstageswasidentifiedinwildemmeraccessionIW170introducedfromIsrael.Anincompletedominantgene,temporarilydesignatedMlIW170,wasresponsiblefortheresistance.ThroughmolecularmarkerandbulkedsegregantanalysesofanF2populationandF3familiesderivedfromacrossbetweensusceptibledurumwheatline81086AandIW170,MlIW170waslocatedinthedistalchromosomebin2BS3-0.84-1.00andflankedbySSRmarkersXcfd238andXwmc243.MlIW170co-segregatedwithXcau516,anSTSmarkerdevelopedfromRFLPmarkerXwg516thatco-segregatedwithpowderymildewresistancegenePm26on2BS.FourEST–STSmarkers,BE498358,BF201235,BQ160080,andBF146221,wereintegratedintothegeneticlinkagemapofMlIW170.ThreeAFLPmarkers,XPaacMcac,XPagcMcta,XPaacMcag,andsevenAFLP-derivedSCARmarkers,XcauG2,XcauG3,XcauG6,XcauG8,XcauG10,XcauG20,andXcauG25,werelinkedtoMlIW170.XcauG3,aresistancegeneanalog(RGA)-likesequence,co-segregatedwithMlIW170.Thenon-glau-cousnesslocusIw1was18.77cMdistaltoMlIW170.Bycomparativegenomicsofwheat–Brachypodium–ricegenomicco-linearity,fourEST–STSmarkers,CJ658408,CJ945509,BQ169830,CJ945085,andoneSTSmarkerXP2430,weredevelopedandMlIW170wasmappedinan2.69cMintervalthatisco-linearwitha131kbgenomicregioninBrachypodiumanda105kbgenomicregioninrice.FourRGA-likesequencesannotatedintheorthologousBrachypodiumgenomicregioncouldserveaschromosomelandingtargetregionsformap-basedcloningofMlIW170.

IntroductionIncoolandhumidareasallovertheworld,powderymil-dew,causedbyBlumeriagraminisf.sp.tritici(Bgt),isaseriousfungaldiseaseofwheataffectinggrainyieldandend-usequality.Inrecentyears,certainagronomicprac-tices,suchaspopularizationofsemi-dwarfcultivars,improvementofirrigationconditions,andincreasinguseofnitrogenousfertilizershaveincreasedcropyield,buthavealsohadtheparadoxicalsideeffectofaggravatingpowdery

CommunicatedbyP.Langridge.Z.LiuandJ.Zhucontributedequallytothiswork.TheseedstockofIW170anditsderivativesareavailableuponrequesttoZhiyongLiu,ChinaAgriculturalUniversityatzhiyongliu@cau.edu.cn.

Z.LiuÁJ.ZhuÁY.CuiÁY.LiangÁH.WuÁW.SongÁQ.LiuÁT.YangÁQ.SunÁZ.Liu(&)StateKeyLaboratoryforAgrobiotechnology,BeijingKeyLaboratoryofCropGeneticImprovement,KeyLaboratoryofCropHeterosisResearchandUtilization,DepartmentofPlantGeneticsandBreeding,ChinaAgriculturalUniversity,Beijing100193,Chinae-mail:zhiyongliu@cau.edu.cn

PresentAddress:W.SongMaizeResearchCenter,BeijingAcademyofAgriculturalandForestrySciences,Beijing100097,China

PresentAddress:Q.LiuNationalAgro-TechExtensionandServiceCenter,Beijing100125,China

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TheorApplGenetDOI10.1007/s00122-011-1767-5mildew.Althoughchemicalagentsareuniversallyusedtocontrolthisdiseaseatpresent,resistantwheatcultivarsarethemosteffective,economical,andenvironmentallysafemeansofprevention.However,race-specificresistancegenestendtoloseeffectivenesswithinshortperiodduetotheselectiveincreaseofvirulentraces.Deploymentofdiversifiedresistancegeneshasbeensuggestedasaremedytothisdilemma,necessitatingasearchfornewpowderymildewresistancegenes.Todate,morethan40wheatpowderymildewresistancelocihavebeenidentified,someofthemderivedfromdiploidortetraploidwildrelativesofcommonwheat(McIntoshetal.2008).Wildemmer,Triticumturgidumvar.dicoccoides(2n=4x=28;genomeAABB),theprogenitorofcultivatedtetraploidandhexaploidwheats,isrichingeneticdiversityforresistancestopowderymildew(Mosemanetal.1984).Severalpowderymildewresistancegenes,suchasPm16(ReaderandMiller1991),Pm26(Rongetal.2000),Pm30(Liuetal.2002),MlZec1(Mohleretal.2005),MlIW72(Jietal.2007),Pm36(Blancoetal.2008),Pm41(Lietal.2009),Pm42(Huaetal.2009),PmG16(Ben-Davidetal.2010),andMl3D232(Zhangetal.2010)wereidentifiedinwildemmer,ortransferredtocultivatedwheatorcultivatedwheatderivatives.Molecularmarkersacceleratetheidentificationandcloningofdiseaseresistancegenesinwheat.Variousmarkers,includingrestrictionfragmentlengthpolymor-phisms(RFLPs),simplesequencerepeats(SSRs),andamplifiedfragmentlengthpolymorphisms(AFLPs),havebeenusedtomapmorethan30powderymildewresistancegenes.Currently,SSRsarethemarkersofchoiceformappinginwheat.Theyareevenlydistributedalongthechromosomesandarepowerfultoolsforgeneticmappingandmarker-assistedselectionofdiseaseresistancegenes.ThousandsofpublicallyavailablewheatSSRmarkershavebeendeveloped(Ro¨deretal.1998;Somersetal.2004;http://wheat.pw.usda.gov).AFLPtechnologyhasthecapabilityofdetectingpoly-morphismsindifferentgenomicregionssimultaneously.Itisalsohighlysensitiveandreproducible.AFLPhasbecomewidelyusedforgeneratinghigh-densitylinkagemapsformajorgenes(Bu¨schgesetal.1997;Hartletal.1999)andquantitativetraitloci(QTLs)(LiuandBai2010).SinceAFLPistime-consumingandcostly,poly-morphicAFLPfragmentscanbeconvertedintosequencetaggedsite(STS)andsequence-characterizedamplifiedregion(SCAR)markers.Expressedsequencetags(ESTs)areconservedportionsofexpressedgenesandthereforecanbeusedtoconductcomparativegenomicsanalyses.ThousandsofwheatESTshavebeenphysicallylocatedtospecificchromosomebinsbyapplyingSouthernhybridizationtoasetofChineseSpringdeletionlines(Qietal.2004).Thesephysically

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向日葵抗F-列当小种分子标记筛选

向日葵抗F-列当小种分子标记筛选

现代农业科技2021年第4期植物保护学摘要利用前期鉴定出来的抗F -小种列当材料和高感列当材料,借助分子标记手段,根据BSA 法建立抗感池,利用分布在17个向日葵连锁群上的409对SSR 引物进行筛选。

结果表明:筛选到了77个多态性SSR 引物;从多态性引物中筛选到4对可将抗-感单株、抗-感混池明确区分开的引物,这些引物可能成为向日葵抗F -列当小种分子标记中的通用引物。

关键词向日葵;列当;分子标记;抗性育种中图分类号S451文献标识码A 文章编号1007-5739(2021)04-0096-02DOI :10.3969/j.issn.1007-5739.2021.04.040开放科学(资源服务)标识码(OSID ):Screening of Molecular Markers of Sunflower Anti-F-broomrape RaceZHANG Ming 1,2HUANG Xutang 1GUAN Hongjiang 1WANG Wenjun 1MA Jun 1MING Yue 3(1Economic Crop Research Institute,Heilongjiang Academy of Agricultural Sciences,Harbin Heilongjiang 150086;2Postdoctoral Research Station of Heilongjiang Academy of Agricultural Sciences,Harbin Heilongjiang 150086;3Heilongjiang Academy of Agricultural Sciences,Harbin Heilongjiang 150086)Abstract Using the anti-F-race and high-sensitivity broomrape materials identified in the earlier stage,with the help of molecular markers,the resistance pool was established according to the BSA method,and 409pairs of SSR primers distributed on 17sunflower linkage groups were screened.The results showed that 77polymorphic SSR primers were screened;from the polymorphic primers,4pairs of primers that could clearly distinguish the anti-susceptible single plant and the anti-sense mixed pool were screened.These primers might become universal primers in the sunflower anti-F-broomrape race molecular markers.Keywords sunflower;broomrape;molecular marker;resistant breeding向日葵抗F -列当小种分子标记筛选张明1袁2黄绪堂1关洪江1王文军1马军1明月3(1黑龙江省农业科学院经济作物研究所,黑龙江哈尔滨150086;2黑龙江省农业科学院博士后科研工作站,黑龙江哈尔滨150086;3黑龙江省农业科学院,黑龙江哈尔滨150086)中国是向日葵传统种植大国,经过20多年的高速发展,目前已经发展成为世界上最大的食用向日葵种植大国和消费大国。

辣椒抗疫病基因初步定位

辣椒抗疫病基因初步定位

Journal of Northeast Agricultural University东北农业大学学报第52卷第3期52(3):20~252021年3月March 2021辣椒抗疫病基因初步定位张曦，王秀雪，邹春蕾*（辽宁省农业科学院蔬菜研究所，沈阳110161）摘要：辣椒疫病是威胁辣椒生产主要病害，改良品种抗性是解决这一问题最有效途径。

选用对辣椒疫霉菌3号生理小种免疫的高代自交系ZCM334为父本抗源，构建F 1、BC 1F 1、F 2群体分析辣椒抗疫病遗传规律性。

结果表明，在感病对照全部发病时，ZCM334对辣椒疫霉菌3号生理小种抗性由一对显性主效基因控制；利用SLAF-BSA 技术对BC 1F 1抗、感极端分离群体混池测序，将辣椒抗疫病基因定位在辣椒第5号染色体5.1Mb 范围内，候选区域内共有26个候选基因，为下一步目标基因精细定位奠定基础。

关键词：辣椒；疫病；SLAF-BSA ；初步定位中图分类号：S641.3；Q781文献标志码：A文章编号：1005-9369（2021）03-0020-06张曦,王秀雪,邹春蕾.辣椒抗疫病基因初步定位[J].东北农业大学学报,2021,52(3):20-25.DOI ：10.19720/ki.issn.1005-9369.2021.03.003.Zhang Xi,Wang Xiuxue,Zou Chunlei.Preliminary mapping of phytophthora blight resistance genes in pepper[J].Journal of Northeast Agricultural University,2021,52(3):20-25.(in Chinese with English abstract)DOI ：10.19720/ki.issn.1005-9369.2021.03.003.Preliminary mapping of phytophthora blight resistance genes inpepper/ZHANG Xi,WANG Xiuxue,ZOU Chunlei(Vegetable Research Institute,LiaoningAcademy of Agricultural Sciences,Shenyang 110161,China)Abstract:Phytophthora blight is the main disease threatening the production of pepper.Improving the resistance of varieties is the most effective method to solve this problem.The high generation inbred line ZCM334,which was immune to Phytophthora capsici No.3physiological species,was used as the paternal parent resistance source.The F 1,BC 1F 1and F 2populations were constructed to analyze the genetic characteristics of pepper resistance to phytophthora blight.The results showed that the resistance of ZCM334to phytophthora blight was controlled by a pair of dominant gene,when all the susceptible controls diseased.SLAF-BSA technique was used to sequencing the resistant and susceptible populations of BC 1F 1.The phytophthora resistance gene was located in the range of 5.1Mb on chromosome 5of pepper.There were 26candidate genes in the candidate region,and it laid the foundation for the further fine mapping of target genesKey words:pepper;phytophthora blight;SLAF-BSA;preliminary mapping 基金项目：辽宁省青年科技创新人才培养专项资金项目（311024）；辽宁省自然科学基金计划重点项目（20170540506）；辽宁省农业重大专项（2019JHI/10200003）作者简介：张曦（1984-），女，助理研究员，博士，研究方向为辣椒抗病育种。

解毒胶囊干预乳腺癌阿霉素耐药信号通路的进展及机制-谷丽艳

解毒胶囊干预乳腺癌阿霉素耐药信号通路的进展及机制-谷丽艳

63第17卷 第12期 2015 年 12 月辽宁中医药大学学报JOURNAL OF LIAONING UNIVERSITY OF TCMVol. 17 No. 12 Dec .，2015解毒胶囊干预乳腺癌阿霉素耐药PTEN-PI3K/AKT信号通路的进展及机制谷丽艳1，张明雪2，张立德1，李佳奕1，解静茹1，刘齐1（1.辽宁中医药大学中医脏象理论及应用教育部重点实验室，辽宁 沈阳 100847；2.辽宁中医药大学附属医院，辽宁 沈阳 110847）摘 要：该文对解毒胶囊治疗乳腺癌的机制进行了研究，主要从阿霉素耐药PTEN-PI3K/AKT 信号通路进行分析探讨，综述了解毒胶囊对乳腺癌阿霉素耐药的近况以及研究进展，说明了解毒胶囊干预乳腺癌阿霉素耐药具有很好的发展前景。

关键词：解毒胶囊；乳腺癌；阿霉素耐药；PTEN-PI3K/AKT 通路中图分类号：R737.9 文献标志码：A 文章编号：1673-842X (2015) 12- 0063- 03收稿日期：2015-04-01基金项目：辽宁省科学事业公益研究基金项目（2014001021）作者简介：谷丽艳（1979-），女，辽宁朝阳人，讲师，博士，研究方向：乳腺增生病的中医治疗。

通讯作者：张明雪（1962-），女，辽宁沈阳人，教授，博士研究生导师，两站博士后，研究方向：中西医结合心血管疾病研究。

Study on Jiedu Capsule Intervention Breast Cancer AdriamycinResistant PTEN-PI3K/AKT Signal PathwayGU Liyan 1，ZHANG Mingxue 2，ZHANG Lide 1，LI Jiayi 1，XIE Jingru 1，LIU Qi 1（1.Key Laboratory of Theoretical and Ministry of Education and Traditional Chinese Medicine，Liaoning University of Traditional Chinese Medicine，Shenyang 100847，Liaoning，China；2.Affialated Hospitalof Liaoning University of Traditional Chinese Medicine，Shenyang 110032，Liaoning，China）Abstract：In this paper，mechanism of Jiedu capsule in the treatment of breast cancer has beenstudied，mainly carries on the analysis discussion from adriamycin resistant PTEN-PI3K/AKT signal pathway，reviewed the progress in the status of Jiedu capsule on breast cancer with adriamycin resistance and research，explained the detoxification capsule intervention breast cancer resistance to doxorubicin has good prospects for development.Key words：Jiedu c apsule；breast cancer；adriamycin resistant；PTEN-PI3K/AKT pathway 一步研发，分子标记等 DNA 水平的鉴定，可将特定的DNA 序列作为探针制成基因芯片，迅速准确对木瓜品种鉴定，相信对木瓜属植物鉴别与研究方面的应用会更加广泛和深入。

基于SYBR Green检测小麦黄花叶病毒的两步法qPCR

基于SYBR Green检测小麦黄花叶病毒的两步法qPCR

基于SYBR Green检测小麦黄花叶病毒的两步法qPCR摘要：小麦黄花叶病毒病是其常发病，常造成严重减产。

为建立可用于定量检测小麦黄花叶病毒（WYMV）拷贝数的qPCR方法，本研究对不同来源的WYMV小种序列进行比对分析，在序列保守区域设计引物11对，对其退火温度进行优化，进而建立各引物的qPCR标准曲线。

结果发现，11对引物的扩增效率在50.60%～116.04%之间，仅2对引物可用于后续研究，扩增效率分别为97.21%（WY-05和WY-06）和91.85%（WY-F2和WY-R2）。

利用其中的WY-F2和WY-R2对高抗和高感WYMV的小麦品种进行分析，结果显示，抗感小麦品种WYMV拷贝数差异极显著。

对抗感分离群体266个单株进行分析，发现该qPCR方法灵敏度显著高于酶联免疫吸附剂测定法，更适用于抗感杂交分离群体的表型鉴定，因而可以用于抗WYMV基因的遗传定位与图位克隆工作。

关键词：小麦黄花叶病毒；两步法qPCR；酶联免疫吸附剂测定法中图分类号：S435.121.4+9文献标识号：A文章编号：1001-4942（2016）07-0118-07小麦黄花叶病毒（Wheat yellow mosaic virus，WYMV）是一种由禾谷多黏菌（Polymyxa graminis）介导才能侵染寄主的土传病原微生物，最早在日本发现[1]，后在中国和加拿大等国发现[2，3]。

感病小麦表现为根系发育差、植株松散[2]、叶片发黄、生长发育迟缓甚至矮化[2，4]，分蘖力、成穗率和产量降低[2，5]，对小麦品质也有不利影响[6]。

我国小麦黄花叶病毒病分布范围广，包括北部冬麦区的胶东沿海副区、黄淮冬麦区大部、长江中下游麦区和西南麦区部分地区等[7]，该病害受寄主感病程度、病毒毒力以及气候因子特别是温度影响较大[5]。

近10年来，我国小麦黄花叶病毒病呈逐年加重之势[2]，仅2006年，就有9个省爆发该病，受灾面积达到200万公顷，造成小麦减产15亿千克[8]。