植物淀粉酶(Amylase)ELISA试剂盒说明书

淀粉分支酶（Starch branching enzyme ，SBE ）试剂盒说明书分光光光度法 50管/24样注 意：正式测定前务必取2-3个预期差异较大的样本做预测定 测定意义：SBE （EC 2.4.1.18）主要存在于植物中，是参与支链淀粉合成的关键酶，测定SBE 活性在淀粉生物合成、优质农作物品种选育和品质遗传改良研究中具有重要意义。

测定原理：直链淀粉和碘结合后在660nm 有特征光吸收，SBE 使直链淀粉含量减少，从而降低了淀粉-碘复合物在660nm 吸收值，一定时间内吸光度下降的百分率可以反映SBE 活性。

需自备的的仪器和用品：可见分光光度计、水浴锅、台式离心机、可调式移液器、1 mL 玻璃比色皿、研钵、冰、蒸馏水试剂的组成和配制：提取液：液体60mL×1瓶，4℃保存； 试剂一：液体20mL×1瓶，4℃保存；试剂二：粉剂×2支，4℃保存；临用前每支加入1mL 蒸馏水，95℃沸水浴充分溶解后备用；用不完的试剂4℃保存；试剂三：液体25mL×1瓶，4℃保存； 试剂四：液体5mL×1瓶，4℃保存；粗酶液提取：按照组织质量（g ）：提取液体积(mL)为1：5~10的比例（建议称取约0.1g 组织，加入1mL 提取液），进行冰浴匀浆。

15000g 4℃离心10min ，取上清，置冰上待测。

测定步骤：混匀，37℃准确保温20 min ，95℃水浴5min （盖紧防止水分散失），冷却试剂名称（μL ）对照管 测定管 95℃水浴1min 后灭活的粗酶液250 粗酶液 250 试剂一 320 320 试剂二3030试剂三500 500试剂四40 40混匀，室温静置10min，用蒸馏水调零，660nm处读取各管吸光值。

注意：1、可以在不同对照管中加入不同样品的粗酶液，然后集中进行5min 95℃水浴处理。

2、试剂二如有沉淀，务必沸水浴溶解后使用。

SBE活力单位的计算1、按照蛋白浓度计算单位的定义：以波长660nm的吸光度下降百分率表示，每mg蛋白在反应体系中每降低1％碘蓝值为一个酶活性单位。

【名称】淀粉酶【英文名】amylase【别名】【概述】淀粉酶(AMY或AMS)全称是1，4-α-D-葡聚糖水解酶，催化淀粉及糖原水解，生成葡萄糖、麦芽糖及含有α1，6-糖苷键支链的糊精。

淀粉酶有α、β两类，人体内淀粉酶属α-淀粉酶，又称淀粉内切酶，不仅作用于淀粉末端，还可作用于其内部的1，4-糖苷键，对1，4-糖苷键连接的葡聚糖有特异性。

但不能水解1，6-糖苷键连接的葡聚糖。

淀粉酶主要由胰腺和唾液腺分泌，肺、肝、甲状腺、脂肪等组织亦含有此酶。

其测定主要为比色法和速率法。

【原理】(1)磺-淀粉比色法：基质中的淀粉经标本中α-淀粉酶水解，生成葡萄糖、麦芽糖和糊精。

剩余淀粉则与碘液结合成蓝色复合物，其颜色的深浅与酶活性成反比。

(2)对-硝基苯麦芽七糖法：对-硝基苯麦芽七糖在AMS作用下，生成一硝基苯麦芽三糖、对硝基苯麦芽四糖、麦芽三糖和麦芽四糖。

前者在α-葡萄糖苷酶作用下，继续水解为对硝基苯酚和葡萄糖，对硝基苯酚生成速率与AMS活性成正比。

【试剂】(1)磺-淀粉比色法：①基质缓冲液(0.4g/L淀粉)：称取NaCl 9g，Na HPO22.6g(Na HPO·12H O 56.94g)和KH PO 12.5g，溶于约500ml蒸馏水中，加热至沸。

另取一个烧杯，准确称取0.4g可溶性淀粉，加入约10ml水，使其混悬后加入上述沸腾之溶液中，水洗烧杯一并倒入。

冷至室温后加入5ml 37%甲醛溶液，用蒸馏水稀释至1000ml。

pH为7.0±0.1，冰箱保存。

②碘贮存液(0.1mol/L)：称取碘酸钾1.7835g，碘化钾22.5g溶于400ml 水中。

缓慢加入浓盐酸4.5ml，边加边搅拌，用蒸馏水稀释至500ml，混匀后置棕色瓶内，冰箱保存。

③碘应用液(0.01mol/L)：取碘贮存液，用蒸馏水稀释10倍，贮存于棕色瓶内，冰箱保存可用1个月。

(2)对-硝基苯麦芽七糖法：主要成分为：3000U/L α-葡萄糖苷酶，5mmol/L对-硝基苯麦芽七糖，100mmol/L磷酸盐缓冲液，50mmol/L NaCl。

血清淀粉酶操作程序1.目的规范血清淀粉酶(AMY)检测试验，确保检测结果准确性和重复性。

2．范围本操作规程适用于生化室工作人员、实习人员、进修人员的操作前培训。

3.术语4．测定原理本试剂基于IFCC 推荐的方法，采用亚乙基-pNP-G7(E-pNP-G7)为底物，防止外酶分解。

其反应原理如下α-淀粉酶5E-pNP-G7 + 5 H 2OE-G3 + pNP-G4 +2 E-G4 +2 pNP-G3 +2 E-G5 +2 pNP-G2 α-葡糖苷酶pNP-G4 +2 pNP-G3 +2 pNP-G2 +14 H 2O 5pNP +14G式中：G 为葡萄糖pNP 为对硝基酚在上述反应中，对硝基酚的生成速率与样本中α-淀粉酶活力成正比，通过在410nm 波长处测定吸光度的上升速率，即可测得样本中α-淀粉酶的活力。

5. 标本采集与处理5.1标本类型：标本最好不要溶血。

留取标本后请尽快分离血清/血浆。

5.2标本稳定性：血清标本在室温下保存可稳定7天，在4℃或-20℃可稳定1个月。

6. 试剂6.1试剂：本科使用上海复星长征医学科学有限公司试剂盒，即用式液体试剂。

试剂内主要成分如下：规格: 4×30ml6．2试剂准备试剂为液体单试剂，开瓶即可使用。

6．3试剂稳定性原装试剂在2～8℃避光保存，稳定期12个月。

试剂开瓶载机2～8℃稳定30天。

7.仪器参数设定AU2700参数设定：长征试剂仪器参数具体参加试剂厂家提供的相应仪器的参数说明书.8．校准：具体参见临床生化校准程序8.1 校准条件：8.1.1仪器光路系统经过光路保养或更换光源等重要部件后。

8.1.2仪器经过大保养后。

8.1.3挪动仪器的安装地点。

8.1.4更换试剂批号。

8.1.5室内质控失控。

8．2 AU2700长征试剂系统的校准：8.2.1 准备：长征配套校准物.8.2.2 保存和稳定性：原校准品在2～8℃保存至有效期。

8.2.3保存位置：2号冰箱。

土壤淀粉酶（S-AL ）活性检测试剂盒说明书可见分光光度法货号：BC1920规格：50T/24S产品组成：使用前请认真核对试剂体积与瓶内体积是否一致，有疑问请及时联系索莱宝工作人员。

试剂名称规格保存条件试剂一液体10 mL×1瓶4℃保存试剂二粉剂×1瓶4℃保存试剂三液体65 mL×1瓶4℃保存标准品粉剂×1支4℃保存溶液的配制：1、试剂二：临用前加入5 mL 蒸馏水，置于常温水中并加热至煮沸，期间不断搅拌或震荡至粉剂溶解。

2、标准品：10 mg 麦芽糖。

临用前加入1.38 mL 蒸馏水配制成20 μmol/mL 的储备液。

产品说明：淀粉酶（EC3.2.1.1）是催化淀粉水解的一类酶的总称。

土壤中的淀粉酶主要来自于微生物，是一种重要的酶制剂，广泛应用于粮食加工、食品、酿造、发酵、纺织品工业和医药行业。

淀粉酶水解淀粉产生还原糖，可与3,5-二硝基水杨酸反应生成红棕色物质，在540nm 处有特征吸收峰，颜色深浅在一定范围内与还原糖量成正比。

注意：实验之前建议选择2-3个预期差异大的样本做预实验。

如果样本吸光值不在测量范围内建议稀释或者增加样本量进行检测。

需自备的仪器和用品：天平、水浴锅、低温离心机、可见分光光度计、1mL 玻璃比色皿、30~50目筛、研钵、甲苯（不允许快递）。

操作步骤：一、样本处理（可适当调整待测样本量，具体比例可以参考文献）新鲜土样自然风干或37℃烘箱风干，过30~50目筛。

二、测定步骤1.分光光度计预热30min ，波长调至540nm ，蒸馏水调零。

2.将20μmol/mL 的麦芽糖储备液用蒸馏水稀释为1、0.9、0.8、0.7、0.6、0.5、0.4、0.3μmol/mL 的标准溶液备用。

3.步骤：试剂名称测定管对照管标准管空白管土样（g ）0.10.1--甲苯（μL ）2020--蒸馏水（μL ）-200--试剂一（μL ）200200--试剂二（μL ）200---Beijing Solarbio Science & Technology Co., LtdTel: 400-968-6088充分震荡混匀，37℃水浴或37℃恒温箱培养24h，之后--12000rpm，常温离心10min，取上清。

迪信泰检测平台
β-淀粉酶检测
β-淀粉酶（β-Amylase），又称淀粉β-1,4-麦芽糖苷酶，是淀粉酶类中的一种，广泛存在于大麦、小麦、甘薯、大豆等高等植物以及芽孢杆菌属等微生物中。

是啤酒酿造、饴糖(麦芽糖浆)制造的主要糖化剂。

迪信泰检测平台采用碘显色法对β-淀粉酶进行高效、精准的检测。

对于相应的酶类物质，我们结合相应的试剂盒处理，采用生化法检测。

此外，我们还提供其他淀粉类的检测服务，以满足您的不同需求。

生化法测定β-淀粉酶样本要求：
1. 请确保样本量大于0.2g或者0.2mL。

周期：2~3周。

项目结束后迪信泰检测平台将会提供详细中英文双语技术报告，报告包括：
1. 实验步骤（中英文）。

2. 相关参数（中英文）。

3. 图片。

4. 原始数据。

5. β-淀粉酶含量/活性信息。

迪信泰检测平台可根据需求定制其他物质测定方案，具体可免费咨询技术支持。

TABLE OF CONTENTSGENERAL INFORMATION (1)Product Description (1)Procedure Overview (1)Kit Contents, Storage and Shelf Life (2)Sensitivity (Detection Limit) (2)Specificity (Cross-Reactivity) (3)Required Materials Not Provided With the Kit (3)Warnings and Precautions (4)SAMPLE PREPARATION (5)Meat/Fish Tissue (5)Milk (5)Milk Powder (5)Butter/Cheese/Curd (6)Yogurt/Buttermilk/Soured Milk/Whey/Sour Cream (6)Serum/Plasma (6)Swipe Sample for Surface Contamination (6)Urine (6)PENICILLIN ELISA TEST KIT PROTOCOL (7)Reagent Preparation (7)ELISA Testing Protocol (8)Penicillin Concentration Calculations (9)TROUBLESHOOTING (10)No Color Development or No Signals with Standards (10)Low Optical Density (OD) Readings (10)High Background or High Optical Density (OD) Readings (10)One or More of the Standard Curve Points Are Out of Range (10)MaxSignal® Penicillin ELISA Test Kit is intended for laboratory use only, unless otherwise indicated. This product isNOT for clinical diagnostic use. MaxSignal is a registered trademark of PerkinElmer (BIOO).MaxSignal® Penicillin ELISA Test Kit – 1065-01BProduct DescriptionMaxSignal® Penicillin ELISA Kit is a competitive enzyme immunoassay for the quantitative analysis of Penicillin and other beta-lactams such as ampicillin, amoxicillin, cloxacillin, and oxacillin. Penicillin is among the most widely used antibiotics for the treatment of bacterial infections in man and animals.Penicillin is also administered to animals in feed for growth promotion and for collective prophylactic treatment. The monitoring of penicillin residues in edible tissues and milk is important because of the hypersensitivity of some individuals to these antibiotics and also the emergence of antibiotic-resistant strains of bacteria. For this reason, maximum residue limits have been specified for food products and milk to try and control the levels of these antibiotics reaching the consumer.MaxSignal® Penicillin ELISA Kit enables international and government regulatory agencies, food manufacturers and processors, as well as quality assurance organizations, to detect penicillin containing antibiotics to satisfy customer concerns about food safety.Procedure OverviewThe method is based on a competitive, two-step colorimetric enzyme-linked immunosorbent assay (ELISA). The target analyte has been coated to the plate wells. During analysis, sample is added along with a primary antibody specific to the target analyte. If the target analyte is present in the sample, it will compete for the antibody, thereby preventing the antibody from binding to the analyte coated to the plate well. A secondary antibody, tagged with a peroxidase enzyme, targets the primary antibody that is complexed to the analyte coated on the plate wells. The resulting color intensity, after addition of TMB substrate, has an inverse relationship to the target analyte concentration in the sample.Kit Contents, Storage and Shelf LifeMaxSignal® Penicillin ELISA Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package.Store the entire kit at 2–8°C. Do not use this product past the expiration date indicated on the Certificate of Analysis.Sensitivity (Detection Limit)Specificity (Cross-Reactivity)•Microtiter Plate Reader with 450 nm primary fi lter and optional 630 nm differential filter (MaxSignal® 4302 Plate Reader Catalog #FOOD-6003-01)•Tissue Homogenizer•Vortex Mixer•10, 20, 100 and 1000 μL Pipettes•Distilled or deionized water•Multi-Channel Pipette: 50–300 μL (Optional)•Reagent Resevoir (Optional)•Hexane, CAS 110-54-3 (ACS-grade or higher recommended)Except as specifically set forth in its terms and conditions of sale, PerkinElmer, Inc. and its subsidiaries (“PerkinElmer”), make no warranty of any kind, either express or implied, with regard to this document or the use of the product, including, but not limited to, the implied warranties of merchantability and fitness for a particular purpose. PerkinElmer shall not be liable for any omissions, or errors or inaccuracies contained herein. PerkinElmer shall not be liable for any damages, including special, consequential or incidental damages in connection with furnishing, performance or use of this material or the productWarnings and PrecautionsPerkinElmer recommends reading the following warnings and precautions to ensure full awareness ofELISA techniques and other details while running the assay. More information can also be found inTroubleshooting section. Periodically, optimizations and revisions are made to the kit and manual.Therefore, it is important to follow the procedures that come with this kit. If further assistance isrequired,****************************************************************************▪The standards contain Penicillin G. Handle with particular care. ▪Do not use the kit past the expiration date. ▪Do not mix reagents from different kits or lots. ▪Standards, antibodies, and plate are kit and lot specific. ▪Ensure any antibodies and corresponding diluents are mixed in correct volumes. ▪Maintain a laboratory temperature of 20 – 25 °C. ▪Avoid running assays under or near air vents, as this may cause excessive cooling, heating and/or evaporation. ▪Do not run assays in direct sunlight, as this may cause excessive heat and evaporation. ▪Do not incubate the plate on a cold surface. Use several layers of paper towel or some other insulation material under the plates during incubation. ▪Use only distilled or deionized water since water quality is very important. ▪When pipetting samples or reagents into an empty microtiter plate, place the pipette tips in the lower corner of the well, making contact with the plastic. ▪Incubations should be timed as precisely as possible. Pipette with a consistent force and rate. ▪Store unused plates at 2–8°C in sealed bags with a desiccant to maintain stability. ▪Allow the plate to equilibrate to room temperature (20–25°C) in the sealed bag to prevent condensation. ▪For small volume reagents (less than 2 mL), after mixing, briefly centrifuge to bring the solution to the bottom of the tubeBe sure samples are properly stored. In general, samples should be refrigerated at 2–8°C. Freezesamples to a minimum of -20°C if they need to be stored for a lo nger period. Frozen samples shouldbe thawed at room temperature (20–25°C) or in a refrigerator before use. For information related toadditionalsamplepreparationmethods,***********************************.1.Preparation of 1X Penicillin Extraction Buffer:Mix 1 volume of 10 X Penicillin Extraction Buffer with 9 volumes of distilled/deionized water.2.Preparation of 1X Balance Buffer:Mix 1 volume of 10 X Balance Buffer with 9 volumes of distilled/deionized water.When preparing solutions, mix vigorously for at least one minute in an air-tight container. Meat/Fish Tissue1.Homogenize a reasonable amount of sample with a suitable mixer until the sample has aconsistency like paste. Store sample at -20°C until analyzed.2.Weigh out 1 g of the homogenized sample and mix with 9 mL of 1X Penicillin G Extraction Bufferthen vortex for 3 minutes or shake for 20 minutes with a shaker.3.Centrifuge for 10 minutes at 4,000 x g at room temperature (20–25°C).4.Take out 1 mL of supernatant and mix it with 2 mL of hexane, vortex for 2 minutes.5.Centrifuge sample for 10 minutes at 4,000 x g.6.Discard the top hexane layer and use 50 μL of the low aqueous layer per well in the test. Be sureto remove the entire upper, hexane layer by careful pipetting.Note: Dilution factor = 10MilkFor fat-free milk, dilute the milk sample 1:4 with 1X Penicillin Extraction Buffer (e.g. 100 μL of milk + 400 μL of 1X Penicillin Extraction Buffer). Use 50 μL of the diluted sample per well for the assay.For regular milk with fat, centrifuge the milk sample at 4,000 x g for 10 minutes and discard the upper fat layer. A toothpick, pipette tip, or weighing spatula is generally adequate to drag the fat layer to the side or pierce an opening through which you may obtain the separated milk. Dilute the milk sample 1:4 with 1X Penicillin Extraction Buffer (e.g. 100 μL of milk + 400 μL of Penicillin Extraction Buffer). Use 50 μL of the diluted sample per well for the assay.Note: Dilution factor = 5Milk PowderTo 1 g of milk powder in a vial, add distilled water to 10 mL, dissolve sample completely by shaking. Then transfer 100 µL of dissolved milk sample above to a new tube, add 400 µL of Penicillin Extraction Buffer. Use 50 µL of the diluted sample per well for the assay.Note: Dilution factor = 5 (corresponding to the dissolved liquid milk)Butter/ Cheese/ Curd1.To 1 g of finely grated cheese or butter, add 4 mL 1X Penicillin Extraction Buffer. (For butter samples, itmay be necessary to heat the sample in a warm water bath for several minutes to form a homogeneous solution before vortexing).2.Vortex vigorously for 5 minutes manually or using a multi-vortexer.3.Centrifuge the samples for 10 minutes at 4,000 rpm.4.Transfer 1 mL of the supernatant to a new tube.e 50 µL of the sample per well for the assay.Note: Dilution factor = 5Yogurt/ Buttermilk/ Soured Milk/ Whey/ Sour Cream1.To 1 mL of the sample add 1 mL of 1X Balance Buffer and 3 mL of 1X Penicillin Extraction Buffer, vortexfor 3 minutes at maximum speed.2.Centrifuge for 5 minutes at 4,000 rpm.e 50 µL of the lower, aqueous layer for the assay (avoid contact with the top fat layer).Note: Dilution factor = 5Serum/Plasma1.Centrifuge the serum/plasma sample at 4,000 rpm for 15 minutes.2.Dilute the supernatant 1:19 with 1X Penicillin Extraction Buffer (e.g. 10 μL of serum/plasma + 190 μL ofBuffer).e 50 μL of the diluted sample per well for the assay.Note: Dilution factor = 20Swipe Sample for Surface Contamination1.Spray 1 mL of 1X Penicillin Extraction Buffer onto the contaminated surface, wipe the surface with onepiece of Kimwipe paper thoroughly.2.Put the Kimwipe paper to a 50-mL plastic tube and add 9 mL of 1X Penicillin Extraction B uffer.3.Vortex for 3 minutes vigorously and leave it at room temperature for at least 15 minutes4.Centrifuge the sample at 4,000 rpm for 15 minutes.e 50 μL of the supernatant per well for the assay.Note: Dilution factor = 10Urine1.Centrifuge the urine sample at 4,000 rpm for 10 minutes.2.Dilute the supernatant 1:9 with 1X Penicillin Extraction Buffer (e.g. 20 μL of urine + 180 μL of buffer)e 50 μL of the supernatant per well for the assay.Note: Dilution factor = 10Reagent PreparationIMPORTANT: All reagents and samples should be brought to room temperature at least 2 hours before use. Make sure to read the “Warnings & Precautions” section. Reagents and samples should be prepared prior to ru nning ELISA. All reagents and samples should be mixed by inverting or vortexing prior to use. Prepare volumes that are needed for the number of wells being run. Do not return any reagents to the original bottles. Aliquotting reagents for individual use and using disposable reservoirs can minimize the risk of contamination and is recommended.1.Preparation of 1X Wash SolutionCombine 1 volume of the 20X Wash Solution with 19 volumes of distilled/deionized water. Mix well.2.Preparation of 1X HRP-Conjugated Antibody #2Combine 1 volume of 200X HRP-Conjugated Antibody #2 with 199 volumes of Antibody #2 Diluent. Vortex for10 seconds to mix. Prepare this solution immediately before performing its addition step in the ELISA.3.Preparation of Penicillin G StandardsAdd 1 mL of 1X Penicillin Extraction Buffer to the stock vial (1,000 ng) and mix by vortexing for 30 seconds to obtain 1,000 ppb stock. Centrifuge the tube briefly to re-collect the liquid. From the stock 1,000 ppb tube take20 μL and dilute it into 980 μL of 1X Penicillin Extraction Buffer to make a 20 ppb stock in a glass vial (pleasedo not use plastic vial). To make the working standards, serially dilute the 20 ppb standard stock vial in the provided empty standard vials as described in the following table. Make sure to mix each standard vial by vortexing thoroughly and briefly centrifuge before removing aliquots.For Preparing Standards for use with milk: Use the Milk Standard Dilution Buffer 1 for all dilutionsNote: The 1,000 ppb stock vial can be divided into single-use aliquots and stored at -20°C in a non-frost-free freezer for at least 1 month. The working standards must be freshly prepared before use in the ELISA. After using the standards, empty the standard vials and store them at 4°C. The empty vials can be re-used for preparing standards for subsequent assays.4.Preparation of 1X Penicillin Capture ProteinMix 1 volume of 10X Penicillin Capture Protein with 9 volumes of Penicillin Capture Protein Diluent. Prepare 5 minutes before use.ELISA Testing Protocol1.Add 50 μL of each Penicillin G standard in duplicate to different wells using a new pipette tip for eachstandard addition. Add standards from low to high concentration.2.Add 50 μL of each sample in duplicate to remaining wells using a new pipette tips for each samp leaddition.3.Add 100 μL of 1X Penicillin Capture Protein to each well.4.Mix the solution in the wells for 1 minute by using a plate shaker or by tapping the plate gently, as to notallow any liquid to spill over.5.Cover the plate. Incubate the plate for 30 minutes at controlled room temperature (20–25°C).6.After incubation, wash the plate 3 times.To wash the plate:a.Decant the liquid thoroughly from each well.b.Add 250 μL of 1X Wash Solution to each well.c.Incubate the wash solution for 15 seconds in each well.d.Decant the wash solution thoroughly from each well.e.Repeat Steps b.–d. two more times.7.After the 3rd wash, invert the plate and forcibly tap it against paper towels until no 1X Wash Solutionremains. Continue to the next step immediately to avoid drying of the plate.8.Add 150 μL of freshly prepared 1X Antibody #2 solution to each well.9.Mix the solution in the wells for 1 minute by using a plate shaker or by tapping the plate gently, as to notallow any liquid to spill over.10.Cover the plate. Incubate the plate for 30 minutes at controlled room temperature (20–25°C).11.After incubation, wash the plate 3 times.To wash the plate:a.Decant the liquid thoroughly from each well.b.Add 250 μL of 1X Wash Solution to each well.c.Incubate the wash solution for 15 seconds in each well.d.Decant the wash solution thoroughly from each well.e.Repeat Steps b.–d. two more times.12.After the 3rd wash, invert the plate and forcibly tap it against paper towels until no 1X Wash Solutionremains. Continue to the next step immediately to avoid drying of the plate.13.Add 100 μL of TMB Substrate to each well. Avoid contamination by not touching the interior of thewells with the pipette tip. Discard the substrate if any coloration is observed before addition to each well.14.Mix the solution in the wells for 1 minute by tapping the plate gently, as to not allow any liquid to spillover.15.Cover the plate. Incubate the plate for 15 minutes at controlled room temperature (20–25°C) in the dark.16.After incubation, add 100 μL of Stop Solution to each wel l to stop the substrate reaction. Avoidcontamination by not touching the interior of the wells with the pipette tip.e a lint-free wipe to clean the bottom of the wells before continuing to the next step.18.Obtain the absorbance values using a plate reader set at 450 nm primary filter (OD450). To decreasebackground with some readings, it is recommended to use an additional 630 nm differential filter simultaneously with each reading.MaxSignal® Penicillin ELISA Test Kit – 1065-01BPenicillin Concentration CalculationsA standard curve can be constructed by plotting the mean relative absorbance (%) obtained from eachreference standard against its concentration in ng/mL on a logarithmic curve.absorbance standard (or sample) x 100Relative absorbance (%) =absorbance zero standardUse the mean relative absorbance values for each sample to determine the corresponding concentration of the tested drug in ng/mL from the standard curve. A special program with Excel functionality, MaxSignal® ELISA Analysis Program in Excel, is available upon request to e valuate the MaxSignal®******************************************************************************** for further informationNo Color Development or No Signals with StandardsLow Optical Density (OD) ReadingsHigh Background or High Optical Density (OD) ReadingsOne or More of the Standard Curve Points Are Out of RangePerkinElmer, Inc. 7050 3913 Todd Lane Suite 312 Austin, TX 78744 USA Tel: 1.888.208.2246 Fax: (512) 707-8122 Made in USABIOO Food & Feed Safety Products****************************。

胰淀粉酶(P-AMY)测定试剂盒（免疫抑制-EPS底物法）适用范围：该产品用于体外定量测定人血清或血浆中胰淀粉酶活性的检测。

1.1 产品规格试剂1:60mL×5，试剂2:12mL×5；试剂1:60mL×2，试剂2:12mL×2；试剂1:60mL×1，试剂2:12mL×1；试剂1:80mL×4，试剂2:16mL×4；试剂1:80mL×2，试剂2:16mL×2；试剂1:50mL×2，试剂2:10mL×2；试剂1:40mL×3，试剂2:8mL×3；试剂1:45mL×5，试剂2:15mL×5；试剂1:45mL×2，试剂2:15mL×2；试剂1:60mL×2，试剂2:15mL×2；试剂1:300mL×1，试剂2:60mL×1；试剂1:20mL×1，试剂2:4mL×1；试剂1:5000mL×1，试剂2:1000mL×1；300人份（试剂1:80mL，试剂2:16mL）；1.2 组成成分试剂1：HEPES-缓冲液PH=7.1 100mmol/L；氯化钠 50mmol/L；氯化镁 10mmol/L；α-葡萄糖苷酶≥23KU/L；单克隆抗体 >35ug/mL；稳定剂 0.09%；试剂2：P-硝基苯D-麦芽庚烷(PNPG7)3mmol/L。

2.1 外观液体双试剂：试剂1：无色透明液体，试剂2：无色透明液体。

2.2 净含量液体试剂的净含量不得低于标示体积。

2.3 试剂空白2.3.1空白吸光度试剂空白吸光度应≤0.5。

2.3.2空白吸光度变化率试剂空白吸光度变化率（ΔA/min）应≤0.004。

2.4 分析灵敏度浓度为100U/L时，吸光度变化率≥0.006。

淀粉酶特别提示：本文内容仅供初步参考，难免存在疏漏、错误等情况，请您核实后再引用。

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目录1 拼音2 英文参考3 概述4 淀粉酶的医学检查4.1 检查名称4.2 分类4.3 取材4.4 淀粉酶的测定原理4.5 试剂4.6 操作方法4.7 正常值4.8 化验结果临床意义4.9 附注4.10 相关疾病5 淀粉酶药品说明书5.1 淀粉酶的别名5.2 性状5.3 淀粉酶的药理作用5.4 适应症5.5 淀粉酶的用法用量5.6 注意事项5.7 规格附：* 淀粉酶相关药品说明书其它版本1 拼音diàn fěn méi2 英文参考amylaseAMYAMS[返回]3 概述淀粉酶（amylase,AMY或AMS）全称是1，4-α-D-葡聚糖水解酶，催化淀粉及糖原水解，生成葡萄糖、麦芽糖及含有α1，6-糖苷键支链的糊精。

一般作用于可溶性淀粉、直链淀粉、糖元等α－1，4-葡聚糖，水解α－1，4-糖苷键的酶。

根据作用的方式可分为α-淀粉酶（EC3．2．1．1．）与β-淀粉酶（EC3．2．1．2．）:（1）α-淀粉酶广泛分布于动物（唾液、胰脏等）、植物（麦芽、山萮菜）及微生物。

微生物的酶几乎都是分泌性的。

此酶以Ca2 为必需因子并作为稳定因子，既作用于直链淀粉，亦作用于支链淀粉，无差别地切断α－1，4-链。

因此，其特征是引起底物溶液粘度的急剧下降和碘反应的消失，最终产物在分解直链淀粉时以麦芽糖为主，此外，还有麦芽三糖及少量葡萄糖。

另一方面在分解支链淀粉时，除麦芽糖、葡萄糖外，还生成分支部分具有α-1，6-键的α-极限糊精。

一般分解限度以葡萄糖为准是35-50％，但在细菌的淀粉酶中，亦有呈现高达70％分解限度的（最终游离出葡萄糖）；（2）β-淀粉酶与α-淀粉酶的不同点在于从非还原性末端逐次以麦芽糖为单位切断α－1，4-葡聚糖链。

大鼠淀粉酶(AMS)酶联免疫分析(ELISA)试剂盒使用说明书齐一生物科技（上海）有限公司本试剂仅供研究使用目的：本试剂盒用于测定大鼠血清，血浆及相关液体样本中淀粉酶(AMS)的含量。

实验原理：本试剂盒应用双抗体夹心法测定标本中大鼠淀粉酶(AMS)水平。

用纯化的大鼠淀粉酶(AMS)抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入淀粉酶(AMS)，再与HRP标记的淀粉酶(AMS)抗体结合，形成抗体-抗原-酶标抗体复合物，经过彻底洗涤后加底物TMB显色。

TMB在HRP酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。

颜色的深浅和样品中的淀粉酶(AMS)呈正相关。

用酶标仪在450nm波长下测定吸光度（OD 值），通过标准曲线计算样品中大鼠淀粉酶(AMS)浓度。

试剂盒组成：试剂盒组成48孔配置96孔配置保存说明书1份1份封板膜2片（48）2片（96）密封袋1个1个酶标包被板1×48 1×96 2-8℃保存标准品：180μmol/L 0.5ml×1瓶0.5ml×1瓶2-8℃保存标准品稀释液 1.5ml×1瓶 1.5ml×1瓶2-8℃保存酶标试剂 3 ml×1瓶 6 ml×1瓶2-8℃保存样品稀释液 3 ml×1瓶 6 ml×1瓶2-8℃保存显色剂A液 3 ml×1瓶 6 ml×1瓶2-8℃保存显色剂B液 3 ml×1瓶 6 ml×1瓶2-8℃保存终止液3ml×1瓶6ml×1瓶2-8℃保存浓缩洗涤液（20ml×20倍）×1瓶（20ml×30倍）×1瓶2-8℃保存样本处理及要求：1. 血清：室温血液自然凝固10-20分钟，离心20分钟左右（2000-3000转/分）。

仔细收集上清，保存过程中如出现沉淀，应再次离心。