Y16_429653-73-6_DataSheet_MedChemExpress

第43 卷 第 5 期2024 年5 月Vol.43 No.5663~673分析测试学报FENXI CESHI XUEBAO （Journal of Instrumental Analysis ）UHPLC-MS/MS 测定化妆品中67种植物提取物标识成分张秋炎，黄芳，梁维维，廖均涛，吴惠勤，罗辉泰*（广东省科学院测试分析研究所（中国广州分析测试中心），广东省化学测量与应急检测技术重点实验室，广东省中药质量安全工程技术研究中心，广东 广州 510070）摘要：建立了超高效液相色谱-串联质谱（UHPLC-MS/MS ）测定化妆品中67种植物提取物标识成分的方法。

化妆品样品经甲醇（含1%甲酸）超声提取，样液在0.1%甲酸溶液-乙腈流动相体系下经Agilent RRHD Eclipse Plus Zorbax C 18（3.0 mm×100 mm ，1.8 μm ）色谱柱梯度洗脱分离。

采用电喷雾正、负离子模式分别对67种成分进行定性定量分析，其中正离子采用多反应监测（MRM ）方式，负离子采用动态多反应监测（DMRM ）方式，以基质匹配外标法定量。

以膏霜、乳液基质为代表，67种成分在各自质量浓度范围内线性关系良好，相关系数（r 2）均大于0.99；检出限为0.002 6~1.7 μg/g ，定量下限为0.008 0~5.0 μg/g ；3个不同加标水平下，膏霜、乳液基质的平均回收率分别为85.6%~117%和82.7%~116%，相对标准偏差（RSD ，n =6）分别为1.0%~12%和0.90%~12%。

该法简单快速，灵敏度高，适用于化妆品中67种植物提取物标识成分的定性鉴定和含量测定。

关键词：超高效液相色谱-串联质谱法；化妆品；植物提取物；标识成分中图分类号：O657.7；TQ658文献标识码：A 文章编号：1004-4957（2024）05-0663-11Determination of 67 Indicative Components from Plant Extracts in Cosmetics by Ultra High Performance Liquid Chromatography-Tandem Mass SpectrometryZHANG Qiu -yan ，HUANG Fang ，LIANG Wei -wei ，LIAO Jun -tao ，WU Hui -qin ，LUO Hui -tai *（Guangdong Provincial Engineering Research Center for Quality and Safety of Traditional Chinese Medicine ，Guangdong Provincial Key Laboratory of Chemical Measurement and Emergency Test Technology ，Institute ofAnalysis ，Guangdong Academy of Sciences （China National Analytical Center ，Guangzhou ），Guangzhou 510070，China ）Abstract ：A new method for the simultaneous and rapid determination of 67 indicative componentsfrom plant extracts in cosmetics by ultra high performance liquid chromatography-tandem mass spec⁃trometry （UHPLC-MS/MS ） was established. The cosmetic samples were dispersed with methanol （1% formic acid ） and ultrasonic extraction. The filtrate was analyzed by a Agilent RRHD Eclipse Plus Zor⁃bax C 18 chromatographic column （3.0 mm×100 mm ，1.8 μm ） with mobile phase consisted of 0.1% formic acid aqueous solution and acetonitrile. 67 indicative components from plant extracts were ana⁃lyzed in the electrospray ionization source ，including positive ion scanning with multiple reaction monitoring mode （MRM ） and negative ion scanning with dynamic multiple reaction monitoring mode （DMRM ） respectively. Creams and lotions were choosed as the representative matrix. The 67 indica⁃tive components showed good linearity with the correlation coefficients （r 2） greater than 0.99 in their respective mass concentration ranges. The detection limits （LODs ） of 67 indicative components were in the range of 0.002 6-1.7 μg/g ，and the quantitation limits （LOQs ） were in the range of 0.008 0-5.0 μg/g. For cream and lotion samples ，the average recoveries of 67 indicative components at three different concentration levels ranged of 85.6%-117% and 82.7%-116%，respectively ； with relative standard deviations （RSDs ，n =6） ranged of 1.0%-12% and 0.90%-12%. The established method isdoi ：10.12452/j.fxcsxb.24010601收稿日期：2024－01－06；修回日期：2024－02－28基金项目：广东省科学院打造综合产业技术创新中心行动资金项目“高端医疗健康与高效生物转化关键技术研究及应用”（2022GDASZH-2022010110）∗ 通讯作者：罗辉泰，副研究员，研究方向：色谱-质谱分析技术应用，E -mail ：luohuitai@研究报告664分析测试学报第 43 卷simple，rapid，effective，high sensitive，and is suitable for the qualitative identification and con⁃tent determination of 67 indicative components from plant extracts in cosmetics.Key words：UHPLC-MS/MS；cosmetics；plant extracts；indicative components随着化妆品市场的不断完善，“安全、天然、绿色”逐渐成为化妆品高质量发展的主题［1］。

蜈蚣药材中3，8-二羟基喹啉的定性定量分析方法研究*刘武占，范建伟，李艳芳，苏瑞强，张贵民**（鲁南制药集团股份有限公司，中药制药共性技术国家重点实验室，山东省中药制药新技术重点实验室，临沂 276006）摘要　目的：建立蜈蚣药材的定性定量分析方法。

方法：采用薄层色谱法对蜈蚣药材进行定性鉴别，以三氯甲烷-甲醇-甲酸（10∶1∶1）为展开剂，在紫外光灯（254 nm）下检视；运用高效液相色谱法测定3，8-二羟基喹啉的含量，采用Kromasil C18（250 mm×4.6 mm，5 μm）色谱柱，流动相为甲醇-10 mmol·L-1的磷酸二氢钾溶液（32∶68），流速0.8 mL·min-1，检测波长252 nm，柱温40 ℃。

结果：薄层鉴别斑点清晰，分离度良好；含量测定中，3，8-二羟基喹啉的线性范围为0.036 2~0.289 6 μg（r=0.999 4），平均加样回收率为99.2%（RSD=1.4%，n=6），17批样品中3，8-二羟基喹啉的含量范围为0.175~2.28 mg·g-1。

结论：本文建立的薄层色谱鉴别及高效液相色谱含量测定方法可用于蜈蚣药材的质量控制。

关键词：动物药材；蜈蚣；喹啉类化合物；3，8-二羟基喹啉；定性鉴别；定量测定；薄层色谱；高效液相色谱中图分类号：R 917 文献标识码：A 文章编号：0254-1793（2017）04-0639-05doi：10.16155/j.0254-1793.2017.04.12Qualitative and quantitative methods of 3，8-dihydroxyquinolone in Scolopendra*LIU Wu-zhan，FAN Jian-wei，LI Yan-fang，SU Rui-qiang，ZHANG Gui-min**（Lunan Pharmaceutical Group Co.，Ltd.，State Key Laboratory of Generic Manufacture Technology of Chinese Traditional Medicine，Shandong Provincial Key Laboratory of New Manufacture Technology of Chinese Traditional Medicine，Linyi 276006，China）Abstract　Objective：To establish qualitative and quantitative methods of Scolopendra. Methods：TLC method was used for qualitative identification of Scolopendra．The plate was developed with chloroform-methanol-formic acid（10∶1∶1）and detected under ultraviolet light at 254 nm．The content of 3，8-dihy droxy quinolone was determined by HPLC．A Kromasil C18（250 mm×4.6 mm，5 μm）column was used，and the mobile phase was methanol-10 mmol·L-1 potassium dihydrogen phosphate（32∶68）at a flow rate of 0.8 mL·min-1．The detection wavelength was set at 252 nm and the column temperature was 40 ℃. Results：TLC spots were clear and well separated．3，8-dihydroxy quinoline showed a good linear relationship at the range of 0.036 2-0.289 6 μg（r=0.999 4）．The average recovery was 99.2%（RSD=1.4%，n=6）and the content range in seventeen batches of samples was 0.175-2.28 mg·g-1．Conclusion：The proposed TLC identification and HPLC assay methods could be used for quality *　山东省自主创新及成果转化专项（创新型产业集群）计划（2015ZDJQ05003） **　通信作者　Tel：（0539）8336001；E-mail：lunanzhangguimin@ 第一作者　Tel：（0539）8336639；E-mail：liuwuzhan@control of Scolopendra.Keywords ：animal medicine ；Scolopendra ；quinoline compounds ；3，8-dihydroxy quinoline ；qualitative identification ；quantitative determination ；TLC ；HPLC 蜈蚣为蜈蚣科动物少棘巨蜈蚣Scolopendra subspinipesmutilans L.Koch 的干燥体，具有息风镇痉，通络止痛，攻毒散结之功效，用于肝风内动，痉挛抽搐，小儿惊风，中风口 ，半身不遂，破伤风，风湿顽痹，偏正头痛，疮疡，瘰疬，蛇虫咬伤［1］；主产于浙江、湖北、江苏、安徽、河南等地［2］。

2021年第10期广东化工第48卷总第444期 · 117· 最新药物治疗幽门螺杆菌感染的最新研究进展谭雨薇，朱艳丽*，薛宇，高家福(佳木斯大学附属第一医院消化内科，黑龙江佳木斯154000)[摘要]幽门螺杆菌是一种革兰氏阴性微需氧细菌，该微生物与上消化道的严重疾病密切相关，被归类为第一类致癌物。

抗生素的耐药性成为幽门螺杆菌感染的主要挑战。

这篇综述提供了幽门螺杆菌治疗的最新进展，进一步研究来解决不同方案在提高幽门螺杆菌根除率方面的作用，尤其是在具有抗生素抗性的菌株中。

[关键词]幽门螺杆菌；铋；沃诺拉赞；益生菌；维生素[中图分类号]TQ [文献标识码]A[文章编号]1007-1865(2021)10-0117-01The Latest Research Progress in the Treatment ofHelicobacter Pylori Infection with the Latest DrugsTan Yuwei,Zhu Yanli*,Xue Yu,Gao Jiafu(Gastroenterology The First Affiliated Hospital of Jiamusi University, Jiamusi 154000, China) Abstract:Helicobacter pylori is a gram-negative microaerophilic bacterium, which is closely related to serious diseases of the upper digestive tract and is classified as the first type of carcinogen. Antibiotic resistance has become the main challenge for Helicobacter pylori infection. This review provides the latest progress in the treatment of Helicobacter pylori and further studies to address the role of different options in increasing the eradication rate of Helicobacter pylori, especially in antibiotic-resistant strains.Keywords:Helicobacter pylori；dual therapy；Vonoprazan；Probiotics；Vitamin幽门螺杆菌(Helicobacter pylori)是革兰氏阴性螺旋形细菌，该菌专门定植于人类的胃上皮，是全世界最常见的感染之一。

Inhibitors, Agonists, Screening LibrariesSafety Data Sheet Revision Date:Mar.-30-2017Print Date:Mar.-30-20171. PRODUCT AND COMPANY IDENTIFICATION1.1 Product identifierProduct name :NVP-TNKS656Catalog No. :HY-13990CAS No. :1419949-20-41.2 Relevant identified uses of the substance or mixture and uses advised againstIdentified uses :Laboratory chemicals, manufacture of substances.1.3 Details of the supplier of the safety data sheetCompany:MedChemExpress USATel:609-228-6898Fax:609-228-5909E-mail:sales@1.4 Emergency telephone numberEmergency Phone #:609-228-68982. HAZARDS IDENTIFICATION2.1 Classification of the substance or mixtureGHS Classification in accordance with 29 CFR 1910 (OSHA HCS)Acute toxicity, Oral (Category 4), H302Acute aquatic toxicity (Category 1), H400Chronic aquatic toxicity (Category 1), H4102.2 GHS Label elements, including precautionary statementsPictogramSignal word WarningHazard statement(s)H302 Harmful if swallowed.H410 Very toxic to aquatic life with long lasting effects.Precautionary statement(s)P264 Wash skin thoroughly after handling.P270 Do not eat, drink or smoke when using this product.P273 Avoid release to the environment.P301 + P312 IF SWALLOWED: Call a POISON CENTER or doctor/ physician if you feel unwell.P330 Rinse mouth.P391 Collect spillage.P501 Dispose of contents/ container to an approved waste disposal plant.2.3 Other hazardsNone.3. COMPOSITION/INFORMATION ON INGREDIENTS3.1 SubstancesSynonyms:TNKS656; TNKS–656Formula:C27H34N4O5Molecular Weight:494.58CAS No. :1419949-20-44. FIRST AID MEASURES4.1 Description of first aid measuresEye contactRemove any contact lenses, locate eye-wash station, and flush eyes immediately with large amounts of water. Separate eyelids with fingers to ensure adequate flushing. Promptly call a physician.Skin contactRinse skin thoroughly with large amounts of water. Remove contaminated clothing and shoes and call a physician.InhalationImmediately relocate self or casualty to fresh air. If breathing is difficult, give cardiopulmonary resuscitation (CPR). Avoid mouth-to-mouth resuscitation.IngestionWash out mouth with water; Do NOT induce vomiting; call a physician.4.2 Most important symptoms and effects, both acute and delayedThe most important known symptoms and effects are described in the labelling (see section 2.2).4.3 Indication of any immediate medical attention and special treatment neededTreat symptomatically.5. FIRE FIGHTING MEASURES5.1 Extinguishing mediaSuitable extinguishing mediaUse water spray, dry chemical, foam, and carbon dioxide fire extinguisher.5.2 Special hazards arising from the substance or mixtureDuring combustion, may emit irritant fumes.5.3 Advice for firefightersWear self-contained breathing apparatus and protective clothing.6. ACCIDENTAL RELEASE MEASURES6.1 Personal precautions, protective equipment and emergency proceduresUse full personal protective equipment. Avoid breathing vapors, mist, dust or gas. Ensure adequate ventilation. Evacuate personnel to safe areas.Refer to protective measures listed in sections 8.6.2 Environmental precautionsTry to prevent further leakage or spillage. Keep the product away from drains or water courses.6.3 Methods and materials for containment and cleaning upAbsorb solutions with finely-powdered liquid-binding material (diatomite, universal binders); Decontaminate surfaces and equipment by scrubbing with alcohol; Dispose of contaminated material according to Section 13.7. HANDLING AND STORAGE7.1 Precautions for safe handlingAvoid inhalation, contact with eyes and skin. Avoid dust and aerosol formation. Use only in areas with appropriate exhaust ventilation.7.2 Conditions for safe storage, including any incompatibilitiesKeep container tightly sealed in cool, well-ventilated area. Keep away from direct sunlight and sources of ignition.Recommended storage temperature:Powder-20°C 3 years4°C 2 yearsIn solvent-80°C 6 months-20°C 1 monthShipping at room temperature if less than 2 weeks.7.3 Specific end use(s)No data available.8. EXPOSURE CONTROLS/PERSONAL PROTECTION8.1 Control parametersComponents with workplace control parametersThis product contains no substances with occupational exposure limit values.8.2 Exposure controlsEngineering controlsEnsure adequate ventilation. Provide accessible safety shower and eye wash station.Personal protective equipmentEye protection Safety goggles with side-shields.Hand protection Protective gloves.Skin and body protection Impervious clothing.Respiratory protection Suitable respirator.Environmental exposure controls Keep the product away from drains, water courses or the soil. Cleanspillages in a safe way as soon as possible.9. PHYSICAL AND CHEMICAL PROPERTIES9.1 Information on basic physical and chemical propertiesAppearance White to light yellow (Solid)Odor No data availableOdor threshold No data availablepH No data availableMelting/freezing point No data availableBoiling point/range No data availableFlash point No data availableEvaporation rate No data availableFlammability (solid, gas)No data availableUpper/lower flammability or explosive limits No data availableVapor pressure No data availableVapor density No data availableRelative density No data availableWater Solubility No data availablePartition coefficient No data availableAuto-ignition temperature No data availableDecomposition temperature No data availableViscosity No data availableExplosive properties No data availableOxidizing properties No data available9.2 Other safety informationNo data available.10. STABILITY AND REACTIVITY10.1 ReactivityNo data available.10.2 Chemical stabilityStable under recommended storage conditions.10.3 Possibility of hazardous reactionsNo data available.10.4 Conditions to avoidNo data available.10.5 Incompatible materialsStrong acids/alkalis, strong oxidising/reducing agents.10.6 Hazardous decomposition productsUnder fire conditions, may decompose and emit toxic fumes.Other decomposition products - no data available.11.TOXICOLOGICAL INFORMATION11.1 Information on toxicological effectsAcute toxicityClassified based on available data. For more details, see section 2Skin corrosion/irritationClassified based on available data. For more details, see section 2Serious eye damage/irritationClassified based on available data. For more details, see section 2Respiratory or skin sensitizationClassified based on available data. For more details, see section 2Germ cell mutagenicityClassified based on available data. For more details, see section 2CarcinogenicityIARC: No component of this product present at a level equal to or greater than 0.1% is identified as probable, possible or confirmed human carcinogen by IARC.ACGIH: No component of this product present at a level equal to or greater than 0.1% is identified as a potential or confirmed carcinogen by ACGIH.NTP: No component of this product present at a level equal to or greater than 0.1% is identified as a anticipated or confirmed carcinogen by NTP.OSHA: No component of this product present at a level equal to or greater than 0.1% is identified as a potential or confirmed carcinogen by OSHA.Reproductive toxicityClassified based on available data. For more details, see section 2Specific target organ toxicity - single exposureClassified based on available data. For more details, see section 2Specific target organ toxicity - repeated exposureClassified based on available data. For more details, see section 2Aspiration hazardClassified based on available data. For more details, see section 212. ECOLOGICAL INFORMATION12.1 ToxicityNo data available.12.2 Persistence and degradabilityNo data available.12.3 Bioaccumlative potentialNo data available.12.4 Mobility in soilNo data available.12.5 Results of PBT and vPvB assessmentPBT/vPvB assessment unavailable as chemical safety assessment not required or not conducted.12.6 Other adverse effectsNo data available.13. DISPOSAL CONSIDERATIONS13.1 Waste treatment methodsProductDispose substance in accordance with prevailing country, federal, state and local regulations.Contaminated packagingConduct recycling or disposal in accordance with prevailing country, federal, state and local regulations.14. TRANSPORT INFORMATIONDOT (US)This substance is considered to be non-hazardous for transport.IMDGUN number: 3077Class: 9Packing group: IIIEMS-No: F-A, S-FProper shipping name: ENVIRONMENTALLY HAZARDOUS SUBSTANCE, SOLID, N.O.S.Marine pollutant: Marine pollutantIATAUN number: 3077Class: 9Packing group: IIIProper shipping name: Environmentally hazardous substance, solid, n.o.s.15. REGULATORY INFORMATIONSARA 302 Components:No chemicals in this material are subject to the reporting requirements of SARA Title III, Section 302.SARA 313 Components:This material does not contain any chemical components with known CAS numbers that exceed the threshold (De Minimis) reporting levels established by SARA Title III, Section 313.SARA 311/312 Hazards:No SARA Hazards.Massachusetts Right To Know Components:No components are subject to the Massachusetts Right to Know Act.Pennsylvania Right To Know Components:No components are subject to the Pennsylvania Right to Know Act.New Jersey Right To Know Components:No components are subject to the New Jersey Right to Know Act.California Prop. 65 Components:This product does not contain any chemicals known to State of California to cause cancer, birth defects, or anyother reproductive harm.16. OTHER INFORMATIONCopyright 2017 MedChemExpress. The above information is correct to the best of our present knowledge but does not purport to be all inclusive and should be used only as a guide. The product is for research use only and for experienced personnel. It must only be handled by suitably qualified experienced scientists in appropriately equipped and authorized facilities. The burden of safe use of this material rests entirely with the user. MedChemExpress disclaims all liability for any damage resulting from handling or from contact with this product.Caution: Product has not been fully validated for medical applications. For research use only.Tel: 609-228-6898 Fax: 609-228-5909 E-mail: tech@Address: 1 Deer Park Dr, Suite Q, Monmouth Junction, NJ 08852, USA。