Abiraterone_metabolite_1_HNMR_28115_MedChemExpress

中国免疫学杂志2023 年第 39 卷川续断皂苷Ⅵ对M1/M2型巨噬细胞极化的调节作用及机制①罗进芳 刘明 杨虹 钱海兵 （贵州中医药大学基础医学院药理学教研室，贵阳 550025）中图分类号 R967 文献标志码 A 文章编号 1000-484X （2023）12-2566-05［摘要］ 目的：研究川续断皂苷Ⅵ对M1/M2型巨噬细胞极化的调节作用及机制。

方法：MTT 法检测川续断皂苷Ⅵ对RAW264.7细胞活力的影响。

ELISA 检测脂多糖（LPS ）诱导状态下RAW264.7细胞上清中TNF -α和IL -6的分泌量；Griess 法检测LPS 诱导状态下RAW264.7细胞上清中一氧化氮（NO ）含量；荧光定量PCR 检测TNF -α、IL -6、精氨酸酶-1（Arg -1）、血红素加氧酶1（HO -1）和细胞因子信号转导抑制蛋白-2（SOCS2）基因表达水平。

Western blot 检测一氧化氮合酶（iNOS ）和p -p65蛋白表达。

结果：在LPS 诱导的RAW264.7细胞中，川续断皂苷Ⅵ抑制TNF -α、IL -6、iNOS 和p -p65蛋白或基因表达水平，同时增加HO -1基因表达。

川续断皂苷Ⅵ能抑制LPS 诱导下RAW264.7细胞分泌的NO 。

川续断皂苷Ⅵ增加IL -4诱导下M2型巨噬细胞标志物Arg1和SOCS2基因表达。

结论：川续断皂苷Ⅵ可以抑制RAW264.7巨噬细胞向M1型极化，同时促进其向M2型极化，可通过调节M1/M2型巨噬细胞极化发挥其抗炎免疫调节作用。

［关键词］ 川续断皂苷Ⅵ；脂多糖；白介素4；巨噬细胞极化Effect and mechanism of Asperosaponin Ⅵ on polarization of M1/M2 macrophagesLUO Jinfang ， LIU Ming ， YANG Hong ， QIAN Haibing. Department of Pharmacology ， Basic Medical College ， Guizhou University of Traditional Chinese Medicine ， Guiyang 550025， China［Abstract ］ Objective ：To study the regulation and mechanism of Asperosaponin Ⅵ on polarization of M1/M2 macrophages. Methods ：MTT assay was used to detect the effects of Asperosaponin Ⅵ on RAW264.7 cell viability. The levels of TNF -α and IL -6 in supernatant of RAW264.7 cells induced by lipopolysaccharide （LPS ） were determined by ELISA. The content of nitric oxide （NO ） in supernatant of RAW264.7 cells induced by LPS was determined by Griess method. The gene expression levels of TNF -α， IL -6， argi⁃nase -1 （Arg -1）， heme oxygenase -1 （HO -1） and suppressor of cytokine signaling protein -2 （SOCS2） were detected by fluorescence quantitative PCR. Western blot was used to detect the expression levels of iNOS and p -p65 protein. Results ：In LPS induced RAW264.7 cells ， Asperosaponin Ⅵ inhibited protein or gene expression levels of TNF -α， IL -6， iNOS and p -p65， and increased HO -1 gene expression. Asperosaponin Ⅵ inhibited NO secretion in RAW264.7 cells induced by LPS. Asperosaponin Ⅵ increased the gene expression levels of M2 macrophage markers Arg1 and SOCS2 induced by IL -4. Conclusion ：Asperosaponin Ⅵ inhibited RAW264.7macrophage polarization to M1 type and promote it polarization to M2 type ， which can play its anti -inflammatory and immunomodulato⁃ry role by regulating M1/M2 macrophage polarization.［Key words ］ Asperosaponin Ⅵ；Lipopolysaccharide ；IL -4；Polarization of macrophages类风湿关节炎（rheumatoid arthritis ， RA ）是一种慢性炎症性自身免疫性疾病，基因和环境因素均会影响其发生发展［1］。

碧云天生物技术/Beyotime Biotechnology 订货热线：400-168-3301或800-8283301 订货e-mail ：******************技术咨询：*****************网址：碧云天网站 微信公众号β-Actin Mouse Monoclonal Antibody产品编号 产品名称包装 AF5001β-Actin Mouse Monoclonal Antibody50µl产品简介：来源 用途 交叉反应性 理论分子量 实际分子量 MouseWB, IF, IHCH, M, R41kDa42kDaWB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC, Immunocytochemistry; FC, Flow Cytometry; ChIP, Chromatin Immunoprecipitation Assay; ChIP-seq, ChIP-sequencing.H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk, Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected.配套提供了Western 一抗稀释液，可以用于Western 检测或其它适当用途时的一抗稀释。

建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整)：包装清单：产品编号 产品名称包装 AF5001 β-Actin Mouse Monoclonal Antibody50µl AZ050 Western 一抗稀释液50ml —说明书1份保存条件：β-Actin Mouse Monoclonal Antibody -20ºC 保存，Western 一抗稀释液-20ºC 或4ºC 保存，一年有效。

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D ATA S HEETRev 120502GMAP1B / MAP5 (Microtubule-Associated Protein) Ab-1 (Clone 3G5)Mouse Monoclonal AntibodyCat. #MS-248-P0, -P1, or -P (0.1ml, 0.5ml, or 1.0ml at 200µg/ml) (Purified Ab with BSA and Azide) Cat. #MS-248-P1ABX or -PABX (0.1ml or 0.2ml at 1.0mg/ml) (Purified Ab without BSA and Azide)Cat. #MS-248-B0, -B1, or -B (0.1ml, 0.5ml, or 1.0ml at 200µg/ml) (Biotin-Labeled Ab with BSA and Azide) Cat. #MS-248-R7 (7.0ml) (Ready-to-Use for Immunohistochemical Staining) Cat. #MS-248-PCS (5 Slides) (Positive Control for Histology)Thermo Fisher Scientific Anatomical Pathology 46360 Fremont Blvd. Fremont, CA 94538, USA Tel: 1-510-771-1560 Fax: 1-510-771-1570 /labvisionThermo Fisher ScientificAnatomical Pathology93-96 Chadwick Road, Astmoor Runcorn, Cheshire WA7 1PR, UKTel: 44-1928-562600 Fax: 44-1928-562627*****************************Manufactured by: NeoMarkers ForLab Vision Corporation Description: Microtubule-associated protein 1B(MAP1B, also known as MAP5, MAP1.2, MAP1(x), or MAP1X) is different from MAP1A (also known as MAP1 or MAP1.1). Reportedly, MAP1B/MAP5 is an early MAP; it is present at high levels in embryonic and newborn rat brain and declines several-fold upon brain maturation. In several cellular situations, MAP1B/MAP5 is the first neuronal MAP to appear, and it is found in neurites from their very first emergence from the cell body. Expression of MAP1B/MAP5 is induced by nerve growth factor.Mol. Wt. of Antigen: 320kDa DoubletEpitope: Not determinedSpecies Reactivity: Human, Cow, and Rat. Doesnot react with chicken. Others-not known.Clone Designation: 3G5Ig Isotype: IgG 1Immunogen: Purified bovine brain MAPsApplications and Suggested Dilutions:• Immunofluorescence• Western Blotting (Not verified)•Immunohistology (Formalin/paraffin) (Ab 1-2µg/ml for 30 min at RT)* [No special pretreatment is required for stainingof formalin-fixed tissues.]The optimal dilution for a specific application should be determined by the investigator.Positive Control: BrainCellular Localization: CytoplasmicStorage and Stability: Ab with sodium azide isstable for 24 months when stored at 2-8°C. Antibody WITHOUT sodium azide is stable for 36 months when stored at below 0°C.Supplied As: 200µg/ml antibody purified from thebioreactor concentrate by Protein G chromatography. Prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide. Also available without BSA and azide at 1mg/ml. Or Prediluted antibody which is ready-to-use for staining of formalin-fixed, paraffin-embedded tissues.Limitations and Warranty:Our products are intended FOR RESEARCH USE ONLY and are not approved for clinical diagnosis, drug use or therapeutic procedures. No products are to be construed as a recommendation for use in violation of any patents. We make no representations, warranties or assurances as to the accuracy or completeness of information provided on our data sheets and website. Our warranty is limited to the actual price paid for the product. NeoMarkers is not liable for any property damage, personal injury, time or effort or economic loss caused by our products.Material Safety Data:This product is not licensed or approved for administration to humans or to animals other than the experimental animals. Standard Laboratory Practices should be followed when handling this material. The chemical, physical, and toxicological properties of this material have not been thoroughly investigated. Appropriate measures should be taken to avoid skin and eye contact, inhalation, and ingestion. The material contains 0.09% sodium azide as a preservative. Although the quantity of azide is very small, appropriate care should be taken when handling this material as indicated above. The National Institute of Occupational Safety and Health has issued a bulletin citing the potential explosion hazard due to the reaction of sodium azide with copper, lead, brass, or solder in the plumbing systems. Sodium azide forms hydrazoic acid in acidic conditions and should be discarded in a large volume of running water to avoid deposits forming in metal drainage pipes.For Research Use Only。