SP-420_911714-45-9_DataSheet_MedChemExpress

Endotoxin Removal Solution Catalog Number E4274Product DescriptionEndotoxins are lipopolysaccharides (LPS), a major component of the Gram-negative bacterial cell wall, and are commonly found as contaminants in plasmid DNA preparations from E. coli. Endotoxins are large, negatively charged molecules that co-purify with DNA on ion exchange and size exclusion columns and in CsCl banding. Endotoxins are extremely potent stimulators of the mammalian immune system and are toxic to primary cells and to animals. The endotoxin toxicity is an obstacle to in vitro and in vivo transfection experiments.Non-ionic detergents, traditionally used for separation of integral membrane proteins,1 can be utilized for removal of endotoxins from DNA solutions by phase separation.2The solubility behavior of a detergent in a dilute, aqueous solution at physiological salt and pH conditions is strongly dependent upon the temperature of the solution. At low temperatures, the detergent forms a clear, micellar solution, but above the cloud point temperature, the micelles form larger, turbid aggregates and ultimately fuse to form a separate phase. The lower phase is detergent-enriched and the detergent-depleted upper phase contains detergent at a concentration slightly above the critical micellar concentration (CMC). Amphiphilic and hydrophobic molecules associated with the micelles of the detergent will aggregate within the detergent-enriched phase, while the soluble, hydrophilic molecules will remain in the detergent-depleted upper phase.Extraction of endotoxin contaminated DNA solutions with the appropriate non-ionic detergent will separate the hydrophilic DNA from the amphiphilic endotoxin. The amphiphilic endotoxin will associate with the lower phase, while the DNA will remain in the upper, detergent-depleted phase.2Reagents and equipment required, but not provided • Water, Molecular Biology Reagent, Catalog Number W4502• E-TOXATE® Water, Catalog Number 2107, or Tris-EDTA (TE) buffer 100×, Catalog NumberT9285• DNA solution (0.5 ml), ~ 1 mg/ml in E-TOXATE®Water or TE buffer• 3 M sodium acetate solution, pH 7.5.• 2-Propanol, Catalog Number I9516, or Ethanol, 190 proof, Catalog Number E7148; 200 proof, CatalogNumber E7023• 70% Ethanol• E-TOXATE®reagents Kits, Catalog Numbers 210A1, 210B1 or 210C1• Ice bucket• Heat block or incubator at 37 °C• Microcentrifuge at room temperature• 1.5 or 2 ml sterile microcentrifuge tubes• Endotoxin-free pipet tips (40-200 µl, 200-1000 µl) Precautions and DisclaimerThis product is for R&D use only, not for drug, household, or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.StorageStore at room temperature.Note: Removal of endotoxins from DNA preparations can be performed either during the final stage of DNApreparation, or during an earlier stage.Procedures for Endotoxin RemovalDuring the final stage of DNA preparationNote: The procedure described below was performed on plasmid DNA produced in E. coli DH5α cells.• Losses of up to 50% of the DNA are expected. • Use of a DNA concentration above therecommended 1 mg/ml reduces the efficiency ofthe procedure.1. Pipette 500 µl of the DNA solution into a sterilemicrocentrifuge tube.2. Add 50 µl of the 3 M sodium acetate solution to theDNA sample.3. Incubate on ice for 5 minutes.4. Add 100 µl of cold Endotoxin Removal Solution.5. Mix thoroughly and incubate on ice for 10 minutes.The solution should be light blue and clear.6. Incubate the tube at 37 °C for 20 to 30 minutes oruntil the phases separate.7. Spin for 5 minutes at 3000 x g in themicrocentrifuge. The upper phase is colorless and clear, while the lower phase is blue.8. Carefully transfer the upper phase containing theDNA to a clean microcentrifuge tube.9. Repeat steps 4 through 8 twice.10. Add 0.6× volume of 2-propanol. Mix by inversion atroom temperature and centrifuge at 15,000 x g for30 minutes at 4 °C. Alternatively, add2.5× volumes of ethanol. Incubate overnight at –20°C or 20 minutes at –70 °C and centrifuge at15,000 x g for 30 minutes at 4 °C.11. Carefully remove the supernatant12. Wash the DNA pellet twice with cold 70% ethanol.Remove the supernatant.13. Air-dry the pellet.14. Suspend the DNA in 100 µl of endotoxin free wateror TE buffer.15. Determine DNA concentration and endotoxin levelsusing endotoxin assay reagents and compare tothe starting material. During an earlier stage of DNA preparationThis procedure is based on the alkaline lysis of E. coli DH5α cells.3 The endotoxins are removed immediately after alkaline cell lysis, neutralization, and a clarification step. The resulting high salt solution is suitable for the endotoxin removal step. It is performed under “endotoxin free” conditions. The plasticware used is either sterile and disposable, or NaOH-treated. The buffers are prepared with endotoxin free water.1. Add the Endotoxin Removal Solution (0.2× volume)to the cold, crude DNA solution.2. Incubate on ice and mix occasionally by inversionto obtain a homogenous, clear blue solution3. Incubate at 37 °C for 20 to 30 minutes until thephase separation is obvious.4. Spin for 5 minutes at low speed (3000 x g) at roomtemperature.5. Transfer the upper aqueous phase to an endotoxinfree container.6. Proceed with the DNA purification by any method.Use endotoxin-free buffers and containers. References1. Bordier, C., J. Biol. Chem., 256, 1604-1607, (1981).2. Cotten, M. et al., Gene Therapy, 1, 239-246,(1994).3. Sambrook et al., Molecular Cloning, a LaboratoryManual, 2nd Ed. p. 1.38RK,PHC 09/05-1Sigma brand products are sold through Sigma-Aldrich, Inc.Sigma-Aldrich, Inc. warrants that its products conform to the information contained in this and other Sigma-Aldrich publications. Purchaser must determine the suitability of the product(s) for their particular use. Additional terms and conditions may apply. Please see reverse side ofthe invoice or packing slip.。

CDCFDA,SE——活细胞荧光示踪探针产品货号包装规格C03625mg储存条件：-20℃，避光保存。

激发/发射波长：504/529nm产品说明书GeneCopoeia,Inc.广州易锦生物技术有限公司广州高新技术产业开发区广州科学城掬泉路3号广州国际企业孵化器F区8楼邮编：510663电话：4006-020-200邮箱：******************网址： ©2016GeneCopoeia,Inc.产品介绍5(6)-CDCFDA,SE是可固定、可渗透细胞的荧光素示踪剂，是一种FDA衍生物，已被广泛证实荧光标记细胞能有效确定总细胞的数量和一个样本中存在多少活细胞。

流式细胞术结合荧光染色法是分析非均质细胞种群的有力工具。

FDA和它的衍生物无荧光分子进入细胞后被胞内无特异性的酯酶所水解产生荧光。

这些荧光产物只能积聚在具有完整细胞膜的细胞中。

因此，死细胞无完整细胞膜不能被染色。

FDA具备精确地膜转运动力学和胞内水解作用或者类似物（CDCFDA）相关的细胞功能，因此可以通过荧光显微镜或者流式细胞仪来检测FDA标记的细胞。

过FDA 荧光标记的细胞在细胞系或者菌株中存在变化，可能是由于不同胞内酯酶的活性所造成的。

活细胞荧光示踪探针CDCFDA，琥珀酰亚胺酯是一个胺化FDA衍生物，可以用来制备各种FDA偶联物。

本品为固体形式，使用前需溶解于DMSO中以配制成储备液。

注意事项配制好的溶液为避免反复冻融，建议适当分装。

对于微量的液体，每次使用前请先高速离心数秒，使液体充分沉降到管底。

荧光染料不可避免的存在淬灭问题，请存放操作时注意避光，以减缓荧光的淬灭。

为了您的安全和健康，请穿实验服并戴一次性手套操作。

产品信息化学名：5-(and-6)-Carboxy-2’,7’-dichlorofluorescein Diacetate,succinimidyl ester分子式：C25H17Cl2NO11分子量：626.36CAS号：147265-60-9操作指南细胞制备与染色注意：不同的实验体系所需的最佳染液工作浓度可能不同，需具体实验中摸索优化。

Proteintech Group,USA, 5400Pearl Street,Suite300, Rosemont,IL60018,USA t1-888-478-4522Proteintech Europe,4th Floor,196Deansgate,Manchester,M33WFt(+44)-161-83-93-007San Ying Biotechnology,China,D3-3,No.666Gaoxin Avenue,Wuhan East LakeHi-tech Development Zone,Wuhan,P.R.C.t86-27-87531629SAFETY DATA SHEET(SDS)1.IDENTIFICATION OF THE SUBSTANCE/PREPARATION AND OF THECOMPANY/UNDERTAKINGProduct Name:CoraLite594-Phalloidin(red)Catalog Number:PF00003Company/undertaking Identification:Proteintech Group5400Pearl StreetSTE300Rosemont,IL60018312-455-8498Emergency telephone number:312-455-8498,ext8022.HAZARDOUS IDENTIFICATION:GHS–ClassificationSignal Word:DangerHazard pictograms:Health hazards:Toxic by inhalation,ingestion,and skin contactPhysical hazards:Not HazardousEnvironmental hazards:Not HazardousHazard Statements:Not ApplicablePrecautionary StatementsPrevention:Not ApplicableResponse:Not ApplicableStorage:Not ApplicableDisposal:Not ApplicableOther hazards:Not ApplicableHMISHealth:2Flammability:0Reactivity:03.INGREDIENT COMPOSITION/INFORMATION:Phalloidin CasNo:None Weight:100%4.FIRST AID MEASURES:Skin Contact Wash off skin thoroughly with soap and water.Remove contaminated clothing and wash before reuse.Eye Contact Ensure adequate flushing of eye contamination for at least15minutes.Inhalation Remove from exposure,rest and keep warm.Ingestion Wash mouth out thoroughly with water and drink plenty of water.Most important symptoms and effects:both acute and delayed Symptoms of allergic reaction may include rash,itching,swelling,trouble breathing,tingling of the hands and feet,dizziness,lightheadedness,chest pain,muscle pain,or flushing.Indication of any immediate medical attention and special treatment needed: Treat symptomatically.Advice for emergency responders:For further assistance,contact your local Poison Control Center.Protection of first-aid responders:Ensure that medical personnel are aware of the material(s)involved,and take precautions to protect themselves.5.FIRE FIGHTING MEASURES:Suitable extinguishing media:Water Spray,Carbon Dioxide,DryChemical Powder,or appropriate foam.Unsuitable extinguishing media:No information availableSpecial hazards arising from the substance or mixture:Not known.Advice for fire-fighters:Standard procedure for chemical fires6.ACCIDENTAL RELEASE MEASURES:Wear appropriate personal protective clothing(Section8).Mop up with an absorbent cloth and arrange removal by a disposal company.Wash site of spillage thoroughly with water and detergent and prevent further leakage or spillage if safe to do so.No special environmental precautions required.7.HANDLING AND STORAGE:Use only in area provided with exhaust ventilation.Avoid contact with skin,eyes and clothing.Wear appropriate protective clothing(Section8).Keep container tightly closed,and in a dark,well-ventilated area.Store at-20℃.8.EXPOSURE CONTROLS/PPE:Follow good laboratory practice when handling this product.Hygiene measures:Handle in accordance with good industrial hygiene and safety practiceRecommended personal protection equipmentEye protection:Laboratory safety goggles.Hands:Chemical resistant gloves.Skin:Laboratory protective clothing.Respiratory:Fume hood or in areas with adequate ventilation.Control Parameters:Not applicable9.PHYSICAL AND CHEMICAL PROPERTIES: Appearance:Colorless liquidOdor:NoneMelting point/melting range:UntestedBoiling point/boiling range:UntestedFlash point:UntestedAutoignition temperature:UntestedDecomposition temperature:UntestedEvaporation rate:UntestedFlammability:UntestedProteintech Group,USA, 5400Pearl Street,Suite300, Rosemont,IL60018,USA t1-888-478-4522Proteintech Europe,4th Floor,196Deansgate,Manchester,M33WFt(+44)-161-83-93-007San Ying Biotechnology,China,D3-3,No.666Gaoxin Avenue,Wuhan East LakeHi-tech Development Zone,Wuhan,P.R.C.t86-27-87531629Upper explosion limit:UntestedLower explosion limit:UntestedVapor pressure:UntestedRelative density:UntestedSpecific gravity:UntestedSolubility:UntestedPartition coefficient:UntestedExplosive properties:Untested10.CHEMICAL STABILITY AND REACTIVITY: Reactivity:None knownChemical stability:Stable under normal conditionsPossibility of hazardous reactions:None reportedConditions to avoid:No information availableIncompatible materials:No dangerous reaction knownHazardous decomposition products:None11.TOXICOLOGICAL INFORMATION:Inhalation:May be irritating to mucous membranes and upper respiratory tract. Eye contact:May cause irritationSkin contact:May cause irritationIngestion:May be harmful if swallowedCarcinogenic effects:Not availableMutagenic effects:Not availableReproductive toxicity:Not availableSTOT–Single exposure:Not availableSTOT–Repeated exposure:Not availableTeratogenic effects:Not available12.ECOLOGICAL INFORMATION:Data not yet available.13.DISPOSAL CONSIDERATIONS:Dispose of waste in accordance with local and national regulations.14.TRANSPORT INFORMATION:IATAUN number UN2811UN proper shipping name Toxic solid,organic,n.o.s.(phalloidin)Transport hazard class(es)6.1Packing group IISpecial provisions A3,A5.15.REGULATORY INFORMATION:US Federal RegulationsSARA313:This product is not regulated by SARA.Clean Air Act,Section112Hazardous Air Pollutants:This product does not contain HAPs.US State RegulationsCalifornia Proposition65:This product does not contain any Proposition65 chemicals.WHMIS Hazard Class:Non-controlled16.OTHER INFORMATION:Reason for revision:New productRevision number:1Revision:2/4/2021The above information is believed to be correct but should only be used as a guide for experienced personnel.Proteintech Group Inc.will not be liable for any damage resulting from the handling of or from contact with the above product.For lab use only,not for diagnostic or therapeutic work.。

聚二甲基硅氧烷msds英文版Material Safety Data SheetSilicon dioxide, dimethylSection 1: IdentificationProduct name: Silicon dioxide, dimethylChemical formula: (CH3)2SiOCAS number: 63148-62-9Synonyms: Dimethicone, Poly(dimethylsiloxane), Silicone oil Section 2: Hazard IdentificationClassification:- Eye irritation category 2- Skin irritation category 2- Specific target organ toxicity - Single exposure category 3 Hazards:- May cause eye irritation.- May cause skin irritation.- May cause respiratory irritation.- May cause drowsiness or dizziness.Section 3: CompositionChemical Name CAS number Concentration Silicon dioxide, dimethyl 63148-62-9 100% Section 4: First Aid MeasuresEye contact:- Immediately rinse eyes with plenty of water for at least 15 minutes, lifting eyelids occasionally. If irritation persists, seek medical attention.Skin contact:- Remove contaminated clothing and wash affected area with soap and water. If irritation occurs, get medical advice.Inhalation:- Move the affected person to fresh air. If breathing becomes difficult, provide oxygen and seek medical attention. Ingestion:- Rinse mouth and drink plenty of water. Do not induce vomiting. Seek medical attention if symptoms occur.Section 5: Fire Fighting MeasuresSuitable extinguishing media:- Use dry chemical, CO2, or foam to extinguish the fire.Section 6: Accidental Release MeasuresPersonal precautions:- Wear appropriate personal protective equipment, such as gloves and safety goggles.Environmental precautions:- Prevent the product from entering drains or water sources. Cleanup methods:- Absorb spill with an inert material, such as sand or vermiculite. Dispose of waste according to local regulations.Section 7: Handling and StorageHandling:- Avoid contact with eyes, skin, and clothing. Use in a well-ventilated area. Do not ingest.Storage:- Store in a cool, dry, well-ventilated area in tightly closed containers. Keep away from heat, sparks, and flame.Section 8: Exposure Controls and Personal Protection Engineering measures:- Ensure adequate ventilation to maintain air levels below occupational exposure limits.Personal protective equipment:- Eye/face protection: Safety glasses or goggles.- Skin protection: Chemical resistant gloves and protective clothing. - Respiratory protection: In case of insufficient ventilation, use NIOSH/MSHA approved respirator.Section 9: Physical and Chemical PropertiesAppearance: Clear, colorless liquidOdor: OdorlessMelting point: -50°CBoiling point: >200°CFlash point: >93°CSpecific gravity: 0.965-0.975 g/cm3Vapor pressure: <0.1 mmHg at 25°CVapor density: >1 (Air=1)Section 10: Stability and ReactivityStability: Stable under normal conditions.Hazardous reactions: None known.Conditions to avoid: Avoid heat, sparks, flame, and other ignition sources.Section 11: Toxicological InformationAcute toxicity:- Oral LD50: >5,000 mg/kg (rat)- Dermal LD50: >5,000 mg/kg (rabbit)Skin irritation:- Rabbit skin irritation: Mild irritantEye irritation:- Rabbit eye irritation: Mild irritantSection 12: Ecological InformationEcotoxicity:- Aquatic toxicity: Not availablePersistence and degradability:- Not readily biodegradableSection 13: Disposal ConsiderationsDisposal methods:- Dispose of waste material in accordance with all local, regional, and national regulations.Section 14: Transport InformationUN Number: Not regulatedUN Proper Shipping Name: Not regulatedTransport hazard class: Not classifiedPacking group: Not applicableSection 15: Regulatory InformationUS federal regulations:- TSCA (Toxic Substances Control Act): Listed- SARA (Superfund Amendments and Reauthorization Act): Not listedSection 16: Other InformationPrepared by: [your company name]Preparation date: [date]Last revision date: [date]Disclaimer: The above information is believed to be accurate, but no guarantee is given or implied. Users must make their own determination of suitability and completeness for their intended use.。