Avobenzone_70356-09-1_DataSheet_MedChemExpress

User GuideBenzonase III ELISA Kit 96-Well PlateEZBNZ3-160KEZBNZ3-160K5PKIntended Use (2)Principles of Assay (2)Reagents Supplied (3)Storage and Stability (3)Reagent Precautions (3)Full Hazard Label (4)Materials Required (5)Reagent Preparation (5)Benzonase III StandardPreparation (5)Preparation of Wash Buffer (7)Preparation of Enzyme Solution . 7Benzonase III ELISA Assay Procedure (7)Assay Procedure for BenzonaseIII ELISA Kit (9)Microtiter Plate Arrangement (10)Assay Characteristics (11)Sensitivity (11)Precision (11)Spike Recovery of Benzonase inAssay Samples (12)Linearity of Dilution (13)Troubleshooting (14)Product Ordering (15)Replacement Reagents (15)Notice (16)Technical Assistance (16)Terms and Conditions of Sale .. 16Contact Information (16)Intended UseThis Benzonase III ELISA kit is used for the non-radioactive quantification of Benzonase. One kit is sufficient to measure 40 unknown samples in duplicate. This kit is for research use only. Not for use in diagnostic procedures.Principles of AssayThis assay is a Sandwich ELISA based, sequentially, on:o Capture of Benzonase molecules from samples to the wells of amicrotiter plate coated with an anti-Benzonase antibodyo Washing of unbound materials from sampleso Binding of a second biotinylated anti-Benzonase antibody to the captured moleculeso Washing of unbound materials from sampleso Binding of streptavidin-horseradish peroxidase conjugate to theimmobilized biotinylated antibodieso Washing of excess free enzyme conjugateso Quantification of immobilized antibody-enzyme conjugates bymonitoring horseradish peroxidase activities in the presence ofthe substrate 3,3’,5,5’-tetramethylbenzidine.The enzyme activity is measured spectrophotometrically by the increased absorbance at 450–590 nm after acidification of formed products. Since the increase in absorbance is directly proportional to the amount of captured Benzonase in the unknown sample, the latter can be derived by interpolation from a reference curve generated in the same assay with reference standards of known concentrationsof Benzonase.Reagents SuppliedEach kit is sufficient to run one 96-well plate and contains the following reagents: Note: Store all reagents at 2-8 °C.Reagents Supplied CatalogueNumber Volume QuantityMicrotiter Plate with 2 plate sealers EP-BNZ3 --------- 1 plate2 sealers Benzonase III Standard E8160-K 100 µL 1 vial Assay Buffer EAB-BNZ 10 mL 1 bottle10X Wash Buffer EWB-HRP 50 mL 2 bottles Benzonase III Detection Antibody E1160-K 12 mL 1 bottle Enzyme Solution (100X) EHRP-BNZ 150 µL 1 bottle Enzyme Solution Diluent ED-BNZ 12 mL 1 bottle Substrate Solution ESS-TMBS1 12 mL 1 bottle Stop Solution ET-TMB 12 mL 1 bottle Storage and StabilityRecommended storage for kit components is 2-8 °C.All components are shipped and stored at 2-8 °C. Reconstituted standards and controls can be frozen for future use but repeated freeze/thaw cycles should be avoided. Refer to expiration dates on all reagents prior to use. Do not mix reagents from different kits unless they have the same lot numbers.Reagent PrecautionsSodium azide or Proclin™ has been added to some reagents as a preservative. Although the concentrations are low, Sodium azide and Proclin™ may react with lead and copper plumbing to form highly explosive metal azides. Dispose of unused contents and waste in accordance with international, federal, state,and local regulations.Full Hazard LabelIngredientComponent NumberFull LabelBenzonase IIIDetection AntibodyE1160-KWarning. Causes serious eye irritation. May cause damage to organsRespiratory Tract through prolonged or repeated exposure. Do not breathe mist or vapours. Wash skin thoroughly after handling. Wear eye protection/ faceprotection. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Get medical advice/attention if you feel unwell. If eye irritation persists: Get medical advice/attention. Dispose ofcontents/container to an approved waste disposal plant.Assay BufferEAB-BNZWarning. Causes serious eye irritation. May cause damage to organsRespiratory Tract through prolonged or repeated exposure. Do not breathe mist or vapours. Wash skin thoroughly after handling. Wear eye protection/ faceprotection. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Get medical advice/attention if you feel unwell. If eye irritation persists: Get medical advice/attention. Dispose ofcontents/container to an approved waste disposal plant.Enzyme Solution (100X)EHRP-BNZWarning. May cause an allergic skin reaction. Avoid breathing mist orvapours. Wear protective gloves. IF ON SKIN: Wash with plenty of water. If skin irritation or rash occurs: Get medical advice/attention. Take off contaminated clothing and wash it before reuse. Dispose of contents/container to an approved waste disposal plant.Ingredient ComponentNumber Full LabelEnzyme Solution Diluent ED-BNZ Warning. May cause an allergic skinreaction. Avoid breathing mist orvapours. Wear protective gloves. IF ONSKIN: Wash with plenty of water. If skinirritation or rash occurs: Get medicaladvice/attention. Take off contaminatedclothing and wash it before reuse.Dispose of contents/container to anapproved waste disposal plant.10X HRP Wash Buffer EWB-HRP Warning. May cause an allergic skinreaction. Wear protective gloves. IF ONSKIN: Wash with plenty of soap.Stop SolutionET-TMB Danger. May be corrosive to metalsMaterials Required(Not Provided)o Multi-channel Pipettes and pipette tips: 5-50 µL and 50-300 µLo Pipettes and pipette tips: 10-20 µL or 20-100 µLo Reagent Reservoirso Polypropylene Microfuge Tubeso Vortex Mixero De-ionized watero Microtiter Plate Reader capable of reading absorbency at 450 nmo Orbital Microtiter Plate Shakero Absorbent Paper or ClothReagent PreparationBenzonase III Standard Preparation1.Take 5 µL of stock standard (5 ug/mL) added to 495 µL of Assay Buffer to makea 50 ng/mL standard concentration.2.Next, take 10 µL of the 50 ng/mL standard concentration prepared above andadd to 490 µL of Assay Buffer. This 1000 pg/mL standard concentration will be Standard 7 in the table below.bel 6 polypropylene microfuge tubes as Std 6, Std 5, Std 4, Std 3,Std 2, Std 1.4.Add 250 µL of Assay Buffer to each of the 6 tubes.5.Prepare serial dilutions by adding 250 µL of the 1000 pg/mL standardconcentration to the number 6 tube, mix well.6.Transfer 250 µL of the number 6 standard to the number 5 tube, mix well.7.Transfer 250 µL of the number 5 standard to the number 4 tube, mix well.8.Transfer 250 µL of the number 4 standard to the number 3 tube, mix well.9.Transfer 250 µL of the number 3 standard to the number 2 tube, mix well.10.Transfer 250 µL of the number 2 standard to the number 1 tube, mix well.11.The 0 standard (Background) will be Assay Buffer.Note: Change tip for every dilution. Wet tip with standard before dispensing. Unused portions of reconstituted standard should be stored in small aliquotsat ≤ -20 °C. Avoid multiple freeze/thaw cycles.Tube # Volume ofAssay Bufferto AddVolume ofStandardto AddStandardConcentrationStandard 6 250 µL 250 µL ofStandard 7 500 pg/mLStandard 5 250 µL 250 µL ofTube # 6250 pg/mLStandard 4 250 µL 250 µL ofTube # 5125 pg/mLStandard 3 250 µL 250 µL ofTube # 462.5 pg/mLStandard 2 250 µL 250 µL ofTube # 331.25 pg/mLStandard 1 250 µL 250 µL ofTube # 215.63 pg/mLTube # Volume ofAssay Bufferto AddVolume of50 ng/mLStandardto AddStandardConcentrationStandard 7 490 µL 10 µL of50 ng/mLstandardconcentration1000 pg/mLPreparation of Wash BufferBring the 10X Wash Buffer to room temperature and mix to bring all salts into solution. Dilute 100 mL of 10X Wash Buffer (two bottles) with 900 mL deionized water. Store unused portion at 2-8 °C for up to one month.Preparation of Enzyme SolutionAdd 120 µL of 100X enzyme solution to the bottle containing 12 mL of enzyme solution diluent. Mix well. Store unused portion at 2-8 °C for up to one month. Benzonase III ELISA Assay ProcedureWarm all reagents to room temperature before setting up the assay.1.Remove the required number of strips from the Microtiter Assay Plate. Unusedstrips should be resealed in the foil pouch and stored at 2-8 °C. Assemble the strips in an empty plate holder. Add 300 µL diluted Wash Buffer to each well of the plate. Decant Wash Buffer and remove the residual volume by inverting the plate and tapping it smartly onto absorbent towels several times. Repeat wash procedure 2 additional times. Do not let wells dry before proceeding to the next step. If an automated machine is used for the assay, follow themanufacturer’s instructions for all washing steps described in this protocol.2.Add 50 µL Assay Buffer to each of the Blank.3.Add 50 µL Standards to the appropriate wells.4.Add 50 µL of sample to the appropriate wells.5.Cover the plate with plate sealer and incubate at room temperature for2 hours on an orbital microtiter plate shaker set to rotate at moderatespeed, about 400-500 rpm.6.Remove plate sealer and decant reagents from the plate. Tap as beforeto remove residual volume in well. Wash wells 3 times with diluted WashBuffer, 300 µL per well per wash. Decant and tap after each wash to removeresidual buffer.7.Add 100 µL Detection Antibody to each well. Re-cover plate with sealer andincubate at room temperature for 1 hour on an orbital microtiter plate shaker set to rotate at moderate speed, approximately 400-500 rpm.8.Remove plate sealer and decant reagents from the plate. Tap as before toremove residual volume in well. Wash wells 3 times with diluted WashBuffer, 300 µL per well per wash. Decant and tap after each wash to removeresidual buffer.9.Add 100 µL Enzyme Solution to each well. Cover plate with sealer and incubatewith moderate shaking at room temperature for 30 minutes on the microtiterplate shaker.10.Remove sealer, decant reagents from the plate and tap plate to remove theresidual volume. Wash wells 5 times with diluted Wash Buffer, 300 µL per well per wash. Decant and tap after each wash to remove residual buffer.11.Add 100 µL of Substrate Solution to each well, cover plate with sealer and shakeon the plate shaker for approximately 30 minutes. Blue color should be formed in wells of the Benzonase III standards with intensity proportional to increasing concentrations of Benzonase.Note: Please be aware that the color may develop more quickly or more slowly than the recommended incubation time depending on the localized roomtemperature. Please visually monitor the color development to optimize theincubation time.12.Remove sealer and add 100 µL Stop Solution (CAUTION: CORROSIVESOLUTION) and gently shake plate by hand to ensure complete mixing ofsolution in all wells. The blue color should turn to yellow after acidification. Wipe the bottom of the microtiter plate to remove any residue prior to reading onplate reader. Read absorbance at 450 nm and 590 nm in a plate reader within5 minutes and ensure that there are no air bubbles in any well. Record thedifference of absorbance units. The absorbance of the highest Benzonase IIIstandard should be approximately 2.0-3.0, or not to exceed the capability of the plate reader used.Note: When sample volumes assayed differ from 50 µL, an appropriatemathematical adjustment must be made to accommodate for the dilution factor (For example, if 25 µL of sample is used, then calculated data must be multiplied by 2). When sample volume assayed is less than 50 µL, compensate for thevolume deficit with Assay Buffer.Assay Procedure for Benzonase III ELISA KitMicrotiter Plate ArrangementBenzonase III ELISA1211 10 98 7 6 5 43 S a m p l e 1 S a m p l e 1 S a m p l e 2 S a m p l e 2 2B l a n k S t a n d a r d 1S t a n d a r d 2S t a n d a r d 3S t a n d a r d 4 S t a n d a r d 5 S t a n d a r d 6 S t a n d a r d 7 1B l a n kS t a n d a r d 1 S t a n d a r d 2 S t a n d a r d 3 S t a n d a r d 4 S t a n d a r d 5 S t a n d a r d 6 S t a n d a r d 7 A B C D E F G HAssay CharacteristicsSensitivityThe lower limit of quantitation(LLOQ) of Benzonase III assay is15.63 pg/mL using BelysaImmunoassay Analysissoftware from MilliporeSigma.LLOQ is calculated by backinterpolation of the standardpoint that provides CV≤20%and recovery ±20%of the expected.PrecisionMean Intra-assay precision is calculated from the results of twenty replicates each of the two different concentrations of Benzonase in a single assay. The mean inter-assay precision is generated from the results of eight separate assays with duplicate samples in each assay for the two different concentrations of Benzonase. Intra-Assay VariationMean Benzonase Levels(pg/mL) Intra-Assay %CV1 478.9 7.102 88.24 7.95Inter-Assay VariationMean Benzonase Levels(pg/mL) Inter-Assay %CV1 392.5 7.242 67.56 6.51Spike Recovery of Benzonase in Assay SamplesVarying amounts of Benzonase were added to assay buffer and the resulting Benzonase content of each sample was assayed by Benzonase III ELISA. The recovery = [(observed- Basal / (spiked Benzonase concentration)] x 100%SampleSpikedConcentrationof BenzonaseAdded(pg/mL)ConcentrationObserved inthe assay(pg/mL)Recovery%1 62.5 209.4 125125 292.2 129250 434.9 1212 62.5 149.2 129125 231.8 130250 379.2 1243 62.5 114.7 130125 191.2 126250 333.5 1204 62.5 211.2 121125 289.5 123250 428.3 1175 62.5 144.3 125125 222.5 125250 358.3 117 Average 124Linearity of Dilution5 samples with the indicated sample volumes were assayed. Neat sample volumes of 50 μL, 25 μL, 12.5 μL, and 6.25 μL in a 50 μL total sample volume represents dilution factors of 1, 2, 4, and 8, respectively. Required amounts of Assay Buffer were added to com pensate for the lost volumes below 50 μL.Dilution linearity = (observed/expected) x 100%Observed = mean calculated dilution corrected concentration at each dilution Expected = mean calculated concentration of the neat sampleSample Neat Samplevolume in50µL totalvolumeMean(pg/mL)DilutionCorrected(pg/mL)Linearity%1 50 551.2 551.225 258.2 516.3 9412.5 115.7 462.7 846.25 62.7 501.8 912 50 235.1 235.125 109.3 218.5 9312.5 54.1 216.2 926.25 28.7 229.3 983 50 531.7 531.725 250.8 501.5 9412.5 115.6 462.2 876.25 63.1 504.7 954 50 246.8 246.825 111.9 223.7 9112.5 54.3 217.2 886.25 29.2 233.3 955 50 101.3 101.325 48.6 97.2 9612.5 24.5 97.8 976.25 14.0 111.9 111 Average 94Troubleshootingo To obtain reliable and reproducible results the operator shouldcarefully read this manual and fully understand all aspects ofeach assay step before attempting to run the assay.o Throughout the assay the operator should adhere strictly to theprocedures with good laboratory practice.o Have all necessary reagents and equipment ready on hand before starting. Once the assay has been started all steps should becompleted with precise timing and without interruption.o Avoid cross contamination of any reagents or samples to be used in the assay.o Make sure all reagents and samples are added to the bottom ofeach well.o Careful and complete mixing of solutions in the well is critical.Poor assay precision will result from incomplete mixing or crosswell contamination due to inappropriate mixing.o Remove any air bubbles formed in the well after acidification ofsubstrate solution because bubbles interfere withspectrophotometric readings.o High signal in background or blank wells could be due to:o cross well contamination by standard solution or sample, oro inadequate washing of wells with Wash Buffer, oro overexposure to light after substrate has been addedProduct OrderingProducts are available for online ordering at . Replacement ReagentsReagents Catalogue Number Microtiter Plate with 2 plate sealers EP-BNZ3 Benzonase III Standard E8160-KAssay Buffer EAB-BNZ10X Wash Buffer EWB-HRP Benzonase III Detection Antibody E1160-KEnzyme Solution (100X) EHRP-BNZ Enzyme Solution Diluent ED-BNZ Substrate Solution ESS-TMBS1Stop Solution ET-TMB Benzonase III ELISA (5 pack bulk) EZBNZ3-160K5PKThe life science business of Merck operates as MilliporeSigma in the U.S. and Canada.Merck, Millipore and Sigma-Aldrich are trademarks of Merck KGaA, Darmstadt, Germany or its affiliates. All other trademarks are theproperty of their respective owners. Detailed information on trademarks is available via publicly accessible resources.NoticeWe provide information and advice to our customers on application technologies and regulatory matters to the best of our knowledge and ability, but without obligation or liability. Existing laws and regulations are to be observed in all cases by ourcustomers. This also applies in respect to any rights of third parties. Our information and advice do not relieve our customers of their own responsibility for checking the suitability of our products for the envisaged purpose.The information in this document is subject to change without notice and should not be construed as a commitment by the manufacturing or selling entity, or an affiliate. We assume no responsibility for any errors that may appear in this document.Technical AssistanceVisit the tech service page at /techservice .Terms and Conditions of SaleWarranty, use restrictions, and other conditions of sale may be found at /terms .Contact InformationFor the location of the office nearest you, go to /offices .。

版本：A4 修改日期：2023.12.18 磷钨酸负染色液(2%,pH7.0)产品简介：负染色又称阴性染色，是由Hall 发现的相对于普通染色(即正染色)而言的染色技术，其原理在于利用重金属盐包绕低电子密度的样品，增强样本四周的电子密度，造成细微结构之间的＂质量－厚度”差异，增强散射吸收反差，使样品在黑暗的背景上呈现明亮的结构，负染色液有磷钨酸、钼酸铵、印度墨汁等，其中最常用的是1～3%磷钨酸。

Leagene 磷钨酸负染色液(2%,pH7.0)适用于显示大分子、细菌、病毒、原生动物、噬菌体、细胞器、核酸大分子、蛋白质晶体及其他大分子材料等，染色后的样品图像呈现透明的亮光，而背景图像呈黑色。

该试剂仅用于科研领域，不适用于临床诊断或其他用途。

产品组成：自备材料：1、 离心机2、 载网3、 显微镜操作步骤(仅供参考)：(一)滴染法1、样品低速离心(2000g ，10min)或采用其他方法浓缩样品，制成悬浮液并且使其达到一定浓度和纯度。

2、将样品悬浮液直接滴于带有支持膜的载网上。

3、用滤纸条从液滴边缘吸去多余液体，稍干燥。

4、 滴加负染色液。

5、吸去多余染色液，自然干燥，进行显微镜观察。

(二)漂浮法1、样品低速离心(2000g ，10min)或采用其他方法浓缩样品，制成悬浮液并且使其达到一定浓度和纯度。

2、将带有支持膜的载网置于样品液滴上漂浮以沾取样品。

编号 名称 DZ0035 Storage 磷钨酸负染色液(2%,pH7.0) 100ml RT 避光 使用说明书 1份3、载网置于负染色液上漂浮1～2min。

4、吸去多余染色液，自然干燥，进行显微镜观察。

染色结果：样品透明的亮光背景黑色注意事项：1、目的样本尽量新鲜。

2、样品应为均匀的悬浮液，其纯度和浓度应适宜，否则无法与染色剂之间产生特异和清晰的结合反应。

3、为了您的安全和健康，请穿实验服并戴一次性手套操作。

4、试剂开封后请尽快使用，以防影响后续实验效果。

Copyright: © 2014 by 'Global Organic Textile Standard International Working Group' © 2014 版权所有人：全球有机纺织品标准国际工作组（IWG）
Contact 联系网址:
GOTS Manual- Issue of 01 March 2014, page 1 of 43 GOTS 手册-2014 年 3 月 1 日发布，第 1 页，共 43 页
Table of contents
目录 Principles........................................................................................................................... 4 总则 ................................................................................................................................... 4 Official interpretations for specific criteria of the GOTS, Version 4.0 .................................. 5 4.0 版 GOTS 特定准则的官方释义 ..................................................................................... 5 1.2 Scope and structure................................

Safety Data Sheet Crafter’s Choice™ White Kaolin ClaySection 1: Identification of the Substance/Preparation and the Company Product Name : Crafter’s Choice™ White Kaolin Clay CAS Number: 1332-58-7 EC Number: 310-194-1 Applications Identified uses: Functional additive Details of the supplier of the safety data sheet: Crafter’s Choice Brands, LLC 7820 E. Pleasant Valley Road Independence, OH 44131 (800) 908-7028 Emergency telephone number: ChemTel (MIS3548100) (800) 255-3924 Domestic USA, Canada, Puerto Rico, USVI + (813) 248-0585 International Section 2: Hazards Identification Classification of the substance or mixture Classification according to Regulation (EC) 1272/2008 (EU 'CLP' regulation) Not classified as hazardous Classification according to Regulation (EC) No. 1272/2008[CLP] Not classified as hazardous. Classification according to Directive 67/5481EEC or 19991451EC Not classified as hazardous. Adverse physiochemical, human health and environmental effects Depending on the type of handling and use (e.g. grinding, drying), airborne respirable crystalline silica may be generated. Prolonged and/or massive Inhalation of respirable crystalline silica dust may cause lung fibrosis, commonly referred to as silicosis.Principal symptoms of silicosis are cough and breathlessness. Occupational exposure to respirable crystalline silica dust should be monitored and controlled. The product is not expected to be hazardous to the environment.Label elements Labelling according to Regulation (EC) 1272/2008 (EU 'CLP' regulation) Risk Phrases NC Not Classified Safety Phrases NC Not ClassifiedRevision Date: 09/25/2015Section 3: Composition and Information on IngredientsSubstancesSection 4: First-aid MeasuresDescription of first aid measuresGeneral advice No acute and delayed symptoms and effects are observed.Inhalation Move-into fresh air and keep at rest. Get medical attention if any discomfort continues.If swallowed Rinse mouth thoroughly. Get medical attention if any discomfort continues.Skin contact Wash skin with soap and water. Use suitable lotion to moisturize skin.Eye contact Do not rub eye. Rinse with large quantities of water. Get medical attention if irritation persists.Most important symptoms and effects, both acute and delayedGeneral informationIf adverse symptoms develop as described, transfer the casualty to a hospital as soon as possible.Indication of any immediate medical attention and special treatment neededNo specific first aid measures noted.Section 5: Fire-fighting MeasuresExtinguishing mediaThis product is non-combustible. No specific extinguishing media is needed.Special hazards arising from the substances or mixtureNon-combustible. No hazardous thermal decomposition.Advice for fire-fightersNo specific fire-fighting protection is required. Use an extinguishing agent suitable for the surrounding fire. Section 6: Accidental Release MeasuresPersonal precautions, protective equipment and emergency proceduresGeneral measuresAvoid airborne dust generation. Wear personal protective equipment incompliance with national legislation.Avoid dry sweeping and use water spraying or vacuum cleaning systems to prevent airborne dust generation. Wear personal protective equipment in compliance with national legislation.Reference to any other sectionsFor personal protection, see section 8. For waste disposal, see section 13.Section 7: Handling and StoragePrecautions for safe handlingAvoid airborne dust generation. Provide appropriate exhaust ventilation at places where airborne dust is generated. In case of insufficient ventilation, wear suitable respiratory protective equipment. Handle packaged products carefully to prevent accidental bursting. If you require advice on safe handling techniques, please contact your supplier or check the Good Practice Guide referred to in section 16. Do not to eat, drink and smoke in work areas; wash hands after use; remove contaminated clothing and protective equipment before entering eating areas.Conditions for safe storage, including any incompatibilitiesStore in a dry covered area. Minimize airborne dust generation and prevent wind dispersal during loading and unloading. Keep containers closed and store packaged products so as to prevent accidental bursting.Specific end use(s)Usage DescriptionIf you require advice on specific uses, please contact your supplier or check the Good Practice Guide referred to in section 16.Section 8: Exposure Controls/Personal ProtectionControl parametersExposure controlsEngineering measuresMinimize airborne dust generation. Use process enclosures, local exhaust ventilation or other engineering controls lo keep airborne levels below specified exposure limits. If user operations generate dust, fumes or mist, use ventilation to keep exposure lo airborne particles below the exposure limit. Apply organizational measures, e.g. by isolating personnel from dusty areas. Remove end wash soiled clothing.Respiratory equipmentIn case of prolonged exposure to airborne dust concentrations, wear a respiratory protective equipment that complies with the requirements of European or national legislation.Use eye protection. Goggles/face shield are recommended. Contact lenses should not be worn when working with this product.Hygiene measuresWhen using, do not eat, drink or smoke. Wash hands at the end of each work shift and before eating, smoking and using the toilet.Use appropriate skin cream to prevent drying of skin.Skin protectionNo specific requirement. Appropriate protection (e.g. protective clothing, barrier cream) is recommended for workers who suffer from dermatitis or sensitive skin.Section 9: Physical and Chemical PropertiesInformation on basic physical and chemical propertiesAppearance LumpColor White / Off-whiteOdor Almost odorlessSolubility Insoluble in waterRelative density 2.6 – 2.7Other InformationNo information requiredSection 10: Stability and ReactivityReactivityNo specific reactivity hazards associated with this product.Chemical stabilityStable under normal temperature conditions and recommended use.Possibility of hazardous reactionsNot applicable.Conditions to avoidNo particular incompatibilityIncompatible materialsNo specific, or groups of materials are likely to react to produce a hazardous situation.Hazardous decomposition productsNone under normal conditions.Section 11: Toxicological InformationInformation on toxicological effectsAcute toxicity This product has low toxicity. Only large volumes may haveadverse impact on human health.Skin corrosion / irritation Prolonged contact may cause dryness of skinSerious eye damage / eye irritation Particles in the eyes may cause inflammation and smarting Respiratory or skin sensitization Dust in high concentrations may irritate the respiratory system Germ cell mutagenicity Not classifiedCarcinogenicity Not classifiedReproductive toxicity Not classifiedSpecific target organ toxicity – single exposure Not classifiedSpecific target organ toxicity – repeated exposure Not classifiedAspiration hazard Not classifiedSection 12: Ecological InformationToxicityLC 50, 96 Hrs. Fish mg/l >1000EC 50, 48 Hrs. Daphnia, mg/l >1000IC 50, 72 Hrs. Algae, mg/l >1000Persistence and biodegradabilityThe product is not biodegradable.Bio-accumulative potentialThe product does not contain any substances expected to be bio-accumulating.Mobility in soilThe product is insoluble in water.Results of PBT and vPvB assessmentNot Classified as PBT/vPvB by current EU criteriaOther adverse effectsEcotoxicityThe product components are not classified as environmentally hazardous. However, this does not exclude the possibility that large or frequent spills can have a harmful or damaging effect on the environment.Section 13: Waste Disposal InformationGeneral InformationThis mineral can be disposed of as a nontoxic/inactive material in approved landfill sites in accordance with local regulations. Dust formation from residues in packaging should be avoided and suitable worker protection assured. Store used packaging in enclosed receptacles. Recycling and disposal of packaging should be carried out in compliance with local regulations. The re-use of packaging is not recommended. Recycling and disposal of packaging should be carried out by an authorized waste management company.Waste treatment methodsWhere possible, recycling is preferable to disposal. Can be disposed of in compliance with local regulations.Section 14: Transport InformationUN NumberNo information requiredTransport hazard class(es)No information required.Packaging groupNo information required.Environmental hazardsEnvironmentally Hazardous Substance / Marine Pollutant - No.Special precautions for userNot applicableTransport In bulk according to Annex U of MARPOL 73178 and IBC CodeNo Information required.Section 15: Regulatory InformationSafety, health and environmental regulations/legislation specific for the substance or mixtureUK Regulatory ReferencesHealth and Safety at Work Act 1974. The Control of Substances Hazardous to Health Regulations 2002 (S.I 2002 No. 2677) with amendments.Statutory InstrumentsThe Chemicals (Hazard Information and Packaging for Supply) Regulations 2009 (S.I 2009 No. 716).Approved Code of PracticeSafety Data Sheets for Substances and Preparations. Classification and Labelling of Substances and Preparations Dangerous for Supply.EU-RegulationsExempted in accordance with Annex V.7National regulationsWorkplace exposure Limits 2005 (EH40)Water hazard classificationNWGChemical safety assessmentNo chemical safety assessment has been carried out.Section 16: Additional InformationGeneral informationWorkers must be informed of the presence of crystalline silica and trained in the proper use and handling of this product as required under applicable regulations.A multi-sectoral social dialogue agreement on Workers Health Protection through the Good Handling and Use of Crystalline Silica and Products Containing It was signed on 25 April 2006. This autonomous agreement, which receives the European Commission’s financial support, is based on a Good Practices Guide. The requirements of the Agreement camehttp://www.nepsi.eu and provide useful Information and guidance for the handling of products containing respirable crystalline silica. Literature references are available on request from EUROSIL, the European Association of Industrial Silica Producers.Prolonged and/or massive exposure to respirable crystalline silica-containing dust may cause silicosis, a nodular pulmonary fibrosis caused by deposition in the lungs of fine respirable particles of crystalline silica.In 1997, IARC (the International Agency for Research on Cancer) concluded that crystalline silica inhaled from occupational sources can cause lung cancer in humans. However it pointed out that not all industrial circumstances, nor all crystalline silica types were to be incriminated. (IARC Monographs on the evaluation of the carcinogenic risks of chemicals to humans, Silica, silicates dust and organic fibers, 1997, Vol. 68, IARC, Lyon, France.)In June 2003, SCOEL (the EU Scientific Committee on Occupational Exposure Limits) concluded that the main effect in humans of the inhalation of respirable crystalline silica dust is silicosis. "There is sufficient information to conclude that the relative risk of lung cancer is increased in persons with silicosis (and, apparently, not in employees without silicosis exposed to silica dust in quarries and in the ceramic industry. Therefore preventing the onset of silicosis will also reduce the cancer risk…” (SCOEL SUM Doc 94 - final, June 2003).So there is a body of evidence supporting the fact that increased cancer risk would be limited to people already suffering from silicosis. Worker protection against silicosis should be assured by respecting the existing regulatory occupational exposure limits and Implementing additional risk management measures where required.Health & Safety Executive: Detailed reviews of the scientific evidence on the health effects or crystalline silica have been published by HSE (Health and Safety Executive, UK) in the Hazard Assessment Documents EH75/4 (2002) and EH75/5 (2003). The HSE points out on its website that Workers exposed to fine dust containing quartz are at risk of developing a chronic and possibly severely disabling lung disease known as "silicosis”. In addition to silicosis, there is now evidence that heavy and prolonged workplace exposure, to dust containing crystalline silica can lead to an increased risk of lung cancer. The evidence suggests that an increased risk of lung cancer is likely to occur only in those workers who have developed silicosis.Disclaimer: The Information in this Safety Advisory Document was obtained from sources which we believe are reliable, but no warranty or representation as to its accuracy or completeness is hereby given. Users should consider the information herein only as a supplement to other information gathered by them and must make independent determinations of suitability and completeness of information from all sources to assure proper use and disposal the safety and health of employees and customers and the protection of the environment.。