免疫胶体金层析法hcg ppt课件

hcg胶体金免疫层析法英文回答：Human Chorionic Gonadotropin (hCG) Colloidal Gold Immunochromatographic Assay.Principle:The hCG colloidal gold immunochromatographic assay is a rapid, qualitative membrane-based immunoassay for the detection of hCG in urine or serum specimens. The test is based on the principle of competitive immunochromatography.A monoclonal antibody specific to hCG is conjugated to colloidal gold particles. When hCG is present in the specimen, it binds to the colloidal gold-labeled antibodies, forming antigen-antibody complexes. These complexes then migrate along the nitrocellulose membrane by capillary action.As the complexes pass through the membrane, theyencounter a second monoclonal antibody specific to hCG immobilized on the test line. The second antibody binds to the antigen-antibody complexes, forming a sandwich complex. The presence of hCG in the specimen is indicated by the appearance of a colored band at the test line.Materials:hCG colloidal gold immunochromatographic test device.Urine or serum specimen.Sample diluent.Test tube or cup.Procedure:1. Remove the test device from its packaging and place it on a flat surface.2. Collect a urine or serum specimen.3. Add 2 drops of the specimen to the sample well on the test device.4. Add 2 drops of sample diluent to the sample well.5. Read the results within 5-10 minutes.Interpretation of Results:Positive Result:Two colored bands appear: one at the control line (C) and one at the test line (T). This indicates that hCG is present in the specimen.Negative Result:Only one colored band appears at the control line (C). This indicates that hCG is not present in the specimen.Invalid Result:No colored bands appear, or a colored band appears only at the test line (T). This indicates that the test is invalid and should be repeated.Limitations:The hCG colloidal gold immunochromatographic assay is a qualitative test and cannot be used to determine the concentration of hCG in a specimen.The test may not detect all cases of pregnancy. A negative result does not rule out pregnancy. If pregnancyis suspected, a quantitative hCG blood test should be performed.The test may be affected by certain medications or medical conditions.中文回答：人绒毛膜促性腺激素 (hCG) 胶体金免疫层析法。