盐胁迫对空心莲子草生长和光合作用的影响
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Botanical Research 植物学研究, 2021, 10(3), 231-238Published Online May 2021 in Hans. /journal/brhttps:///10.12677/br.2021.103033盐胁迫对植物光合–水分关系的影响研究周丹浙江师范大学化学与生命科学学院,浙江金华收稿日期:2021年4月7日;录用日期:2021年5月8日;发布日期:2021年5月18日摘要随着环境恶化,盐胁迫日益成为一个全球问题。
盐胁迫影响植物的水分运输和光合作用过程,导致植株生长缓慢甚至死亡。
植物在盐胁迫下水分运输效率降低,造成光合作用原料之一水供应不足,引起光合作用受抑制。
光合作用受抑制导致植物碳同化量积累不够不足以维持植物生长,从而影响植物的生长和发育。
总结盐胁迫对植物的水分状况和光合作用的影响及作用机制,为探究植物在盐胁迫下的适应机制提供参考,对植物在盐滞化土壤中更好地生长发育提供理论基础。
关键词盐胁迫,光合作用,水分运输Effects of Salt Stress on Photosynthesis and Water in PlantsDan ZhouCollege of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua ZhejiangReceived: Apr. 7th, 2021; accepted: May 8th, 2021; published: May 18th, 2021AbstractWith the deterioration of the environment, salt stress is becoming a global problem. Salt stress af-fects water transport and photosynthesis of plants and causes slow growth and even death of plants. Under salt stress, water transport efficiency of plants is reduced, resulting in insufficient water supply as one of the raw materials for photosynthesis, resulting in inhibition of photosyn-thesis. Inhibited photosynthesis leads to insufficient accumulation of carbon assimilation in plants,周丹which is insufficient to maintain plant growth, thus affecting plant growth and development. In this paper, the effects of salt stress on water status and photosynthesis of plants and their me-chanisms of action were summarized to provide reference for exploring the adaptation mechan-ism of plants under salt stress, and to provide a theoretical basis for better growth and develop-ment of plants in salt-retarded soil.KeywordsSalinity, Photosynthesis, Water Transport Array Copyright © 2021 by author(s) and Hans Publishers Inc.This work is licensed under the Creative Commons Attribution International License (CC BY 4.0)./licenses/by/4.0/1. 引言植物在生长过程中难免会受到环境或生物方面的胁迫,盐胁迫是影响植物生长和产量的重要环境因子之一[1]。
空心莲子草具有较强抗盐能力的原因初探
高建明;肖强;崔翠菊;何光源
【期刊名称】《生物技术通报》
【年(卷),期】2006(000)C00
【摘要】为了探讨空心莲子草(Alternanthera philoxeroides(Mart.)Griseb)的抗盐机理,测定了盐胁迫条件下的空心莲子草和水稻中保护酶活性和渗透调节物质的变化。
结果表明,在盐胁迫下,空心莲子草中的脯氨酸、甜菜碱以及SOD、POD等含量增加速度比水稻快,膜脂过氧化产物丙二醛的含量在两种植物中虽亦
增加,但在空心莲子草叶中的增加幅度小于水稻。
证明盐胁迫下保护酶活性和渗透调节物质含量的迅速增加是空心莲子草具有较强抗盐能力的重要生理学基础。
【总页数】4页(P391-394)
【作者】高建明;肖强;崔翠菊;何光源
【作者单位】华中科技大学中英HUST-RRes基因工程和基因组学联合实验室,武
汉430074
【正文语种】中文
【中图分类】S451
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重金属胁迫条件下空心莲子草的生长和营养特征分析宋志忠;王莉;金曼;苏彦华【期刊名称】《广西农业生物科学》【年(卷),期】2011(030)005【摘要】Alternanthera philoxeroides,usually called as Shui-hua-sheng in China and commonly known as Alligator weed in English,is an immersed aquatic plant and is listed an invasive species in China.In this work,we found that Shui-hua-sheng(A.philoxeroides) is able to accumulate six heavy metals with the ability of Zn2+Mn2+Pb2+Cu2+Cd2+Cr3+.Under the conditions of the stimulated external high concentration(1 mmol/L)of Cu2+,Mn2+,Zn2+ and Cr3+,the biomass of Shui-hua-sheng(A.philoxeroides) reduced,total length of root and total area of root surface decreased as well,accordingly R/S ratios increased.Moreover,the nutriational characteristics of K+,Ca2+ and Mg2+ were altered significantly,respectively,under the exposure to contaminations of Pb2+,Cd2+,Cu2+ and Zn2+.The findings in this research implied that the Shui-hua-sheng(A.philoxeroides) be able to tolerate mentioned heavy-metals with high level contamination,and also might be as alternative to be used for biomediation of heavy-metal polluted soil or water.%空心莲子草是一种常见的水生植物,并能在重金属污染的水体或附近土壤中生长。
盐胁迫对植物生长的影响Abstract Soil salinization affects the whole growth cycle of plant,and has become an ecological issue attracting global attention.In this research,the effects of salt stress on plant seed germination,morphological development,photosynthetic characteristics,physiological and biochemical characteristics and ion homeostasis were summarized,which provided relevant theoretical references for the researches on soil salinization and salt resistance breeding.Key words Salt stress;Plant growth;Physiological and biochemical characteristics土壤鹽渍化是限制农业发展的一项重要生态因素,始终影响着植物的整个生命周期,其对植物的影响是一个综合复杂的过程。
土壤中高浓度的盐分离子不仅造成土壤水势下降,推迟或抑制植物种子萌发,而且还会抑制该环境中的植物幼苗根、茎、叶、花、果实等器官的生长发育和代谢过程,使植物生长受到抑制;当盐分离子进一步升高时,除因水势降低而对植物产生渗透胁迫外,还对植物产生离子胁迫,破坏细胞中离子平衡,抑制酶活性的表达,限制营养物质的供应,干扰细胞中离子代谢,改变其组织和细胞的显微和超微结构,此外还限制作物根系对盐分离子的吸收和运输机制,造成离子毒害,降低植物叶绿素含量,进而阻止光合作用的正常进行。
盐胁迫对植物的影响及植物的抗盐机理摘要: 盐是影响植物生长和产量的主要环境因子之一, 根据国内外最新的研究资料, 从盐胁迫对植物的生长、水分关系、叶片解剖学、光和色素及蛋白、脂类、离子水平、抗氧化酶及抗氧化剂、氮素代谢、苹果酸盐代谢、叶绿体超微结构的影响, 及影响光合作用的机制等方面入手, 对植物盐胁迫研究现状及进展情况进行了综述, 旨在为开展植物抗盐机理研究、选育培育耐盐植物新品种提供依据。
关键词: 植物盐胁迫抗盐性机理Effects of Salt Stress on Plants and the Mechanism of Salt ToleranceAbstract: Salinity is the major environmental factor limit ing plant growth and productivity. According to the documents and data at home and abroad, the research currents of salt stress in plants were summarized including the effect on plant growth, the water relations, leaf anatomy, photosynthetic pigments and proteins, lipids, ion levels, antioxidative enzymes and antioxidants etc. This r eview may help to study the salt2toler ant mechanism and breeding new salt-toler ant plants.Key words: plant, salt2stress, salt2tolerant, mechanism目前, 受全球气候变化、人口不断增长的影响,土壤盐碱化日趋严重。
盐胁迫对植物影响摘要:土壤盐渍化是现代农业生产所面临的主要问题之一。
植物为了抵御盐分胁迫,它们积极地适应生存环境,产生了一系列生理生化的改变以调节水分及离子平衡,维持正常的光合作用。
本文主要从盐胁迫对植物细胞生理生化的影响、植物对盐的适应性及抗盐机理和盐对种子萌发的影响,在Nacl胁迫下,对种子发芽势、发芽率、发芽指数、活力指数等问题进行分析,探讨植物种子在不同盐分浓度下的耐盐性和提高植物的耐盐性,减轻土壤盐渍化危害。
关键词:Nacl胁迫;发芽率;发芽势;土壤盐渍化To Summarize on Salt Stress on PlantsAbstract:Soil salinization is one of the main problems facing in a modern agriculturalproduction .Plants to resist salt stress, they actively adapt to the living environment,a series of physiological and biochemical changes in order to regulate water and ion balance and maintain normal photosynthesis. This article from the salt stress on plant cell physiology and biochemistry of plant adaptation to salt and salt tolerance mechanisms and the influence of salt on seed germination in Nacl stress on seed germination potential,germination rate,germination index,vigor index Problems are analyzed to explore the seeds under different salinity tolerance and improve the salt tolerance of plants to reduce soil salinity hazards.Key words:Nacl stress;germination rate ;ermination energy;soil salinization 土壤盐渍化是人类面临的生态危机之一,土壤的盐碱化问题日益威胁着人类赖以生存的有限的土地资源。
入侵植物空心莲子草早期生长对盐胁迫的响应刘大胜1*,娄美芳2,范海2,范舒月2,尹济滨1,杨淑英1,孔立志1(1.山东省环境保护科学研究设计院,山东济南250013;2.山东师范大学生命科学学院,山东济南250014)摘要[目的]研究入侵植物空心莲子草早期生长对盐胁迫的响应。
[方法]用分别含有浓度为0(对照)、100、200、300mmol/L NaCl 的1/2浓度Hoagland 营养液处理早期生长阶段的空心莲子草,研究NaCl 处理对空心莲子草的茎节数目、叶片数目、茎长、鲜重和干重和含水量的影响。
[结果]NaCl 处理对空心莲子草的茎节数目、叶片数目、茎长、鲜重和干重均产生影响,随着处理浓度的升高影响逐渐显著。
中高浓度的NaCl 处理能显著抑制空心莲子草的生长,主要表现为抑制空心莲子草的茎节数目、茎长、鲜重和干重的增长,减少空心莲子草的叶片数目,其中茎长和鲜重是2个较易受影响的指标。
[结论]开展空心莲子草的抗盐研究对了解其对盐碱地环境的适应性,防止其在盐碱地环境的入侵具有重要意义。
关键词空心莲子草;早期生长;盐胁迫;响应基金项目山东省科技攻关计划(2009GG10008014);山东省省级环保研发专项(2011小清河)。
作者简介刘大胜(1968-),男,安徽合肥人,硕士,研究员,从事生态学研究,E 鄄mail :*****************。
*通讯作者。
收稿日期2013鄄08鄄10修回日期2013鄄09鄄05Response of Invasive Plant Alligatorweed to Salt Stress at Early Growth StageDasheng LIU 1鄢,Meifang LOU 2,Hai FAN 2,Shuyue FAN 2,Jibin YIN 1,Shuying YANG 1,Lizhi KONG 11.Shandong Institute of Environmental Science,Ji ’nan 250013,China;2.College of Life Science,Shandong Normal University,Ji'nan 250014,ChinaSupported by Shandong Science and Technology Program (2009GG10008014);Shandong Environmental Protection Research and Development Project (2011Xiaoqinghe).鄢Corresponding author.E 鄄mail:*****************Received:August 10,2013Accepted:September 5,2013AAgricultural Science &Technology,2013,14(9):1325-1327,1346Copyright 訫2013,Information Institute of HAAS.All rights reservedResources and EnvironmentAbstract [Objective ]The study aimed to investigate the early growth stage ’s re 鄄sponse of invasive plant alligatorweed (Alternanthera philoxeroides )under salt stress.[Method ]The alligatorweed was cultivated with four different NaCl concentrations 0(control),100,200,300mmol/L diluted in 1/2Hoagland ’s solution at early stage.During the period,we measured the number of nodes and leaves,length,fresh weight,dry weight and water content of stems to determine the effect of salt on al 鄄ligatorweed growth.[Result ]Compared with control,the node number,leaf number,length,fresh weight and dry weight of the stems significantly changed in NaCl solu 鄄tion,and the difference was positively related to NaCl concentration.We concluded that middle and high concentrations of NaCl significantly inhibited the growth of alli 鄄gatorweed,by suppressing the node number,leaf number,length,fresh weight and dry weight of the stems.Among them,the stem length and fresh weight were more sensitive to NaCl stress.[Conclusion ]The present result is helpful to understand the alligatorweed ’s adaptability to saline environment,and also helpful to prevent its in 鄄vasion in such environment.Key words Alligator weed;Early growth;Salt stress;ResponseBiological invasion is a serious environmental and economic problem in the world [1-3].In thepast 20years,the number of invasive species that have invading China is increasing,and they extended to more regions and caused more serious ecological damage and severer eco 鄄nomic losses.To prevent the damage of the invasive species,Alternanthera philoxeroides (Mart.)Griseb and other 15invasive species requiring special control were announced in China in 2003[4].Alligatorweed originated in Pa 鄄rana River region of South America [5-6]and now has spread to South Ameri 鄄ca,North America,Asia,Australia and the neighboring islands [7].It was intro 鄄duced to China ’s mainland in the late 1930s as horse feed by Japanese in Shanghai suburbs,and unfortunately it escaped from cultivation and became a significant ecological problem.Alli 鄄gatorweed distributes widely and causes serious damage in the middle and lower reaches of Yangtze River [9].However,we found it significantly in 鄄vaded the upper Xiaoqing River in Shandong Province of northern China in 2005-2006and caused a damage to local river [10-11].At present,the re 鄄search work of plant salt resistance is mainly focused on wheat (Triticum aestivum ),corn (Zea mays ),thale cress (Arabidopsis thaliana ),and mal 鄄low (Malva sinensis ),etc .[12-16],but rarely found on alligatorweed.There is large areas of saline 鄄alkali soil in north of Xiaoqing River and its middle and low 鄄er reaches.The research on alligator 鄄weed ’s resistance to salt will help to understand the alligatorweed ’s adapt 鄄ability in saline 鄄alkali environment,and also provide reference for preventing its invasion in saline 鄄alkali environ 鄄ment.In addition,as alligatorweed is easy to reproduce and grows fast,we also discussed the probability to use it2013Fig.1The growth status of alligatorweedtreated by 0,100,200and 300mmol/L of NaCl,from left to rightThe ratio of node number=The node num 鄄ber measured on the 1st ,4th ,7th ,and 10th days/the node number measured on the 1st day.So the ratio of node number on the 1st day was recorded 1;the different letters represent significant differences (P <0.05).Fig.2Ratio of node number of alligatorweedtreated by different concentrations of NaClThe different letters represent significant dif 鄄ferences (P <0.05).Fig.3Leaf number of alligatorweed treatedby different concentrations of NaClas experimental material here.Materials and MethodsMaterialsThe wild alligatorweeds were col 鄄lected in middle 鄄late April from a branch river near upper Xiaoqing Riv 鄄er,grown in plastic pots containing 1/2Hoagland ’s solution in a greenhouse,while water was sprayed every day.Three 鄄week 鄄later,stems were cut off and treated with different concentra 鄄tions of NaCl solution.The Hoagland ’s solution con 鄄tained 5ml of Ca(NO 3)2,5ml of KNO 3,2ml of MgSO 4,1ml of KH 2PO 4,1ml of Fe-EDTA and 1ml of trace element.Experimental methodsAll the alligatorweed stems to be tested were 15-17cm in length,ap 鄄proximately 2mm in diameter,about 1.7-1.8g in weight,with 3-5nodes and 7-12leaves.We selected the stems similar in length,diameter,weight,number of nodes,leaf number and size,grouped them at random.Their length,weight,number of nodes and leaves were measured and recorded during the following days.The length of the stems was measured using cot 鄄ton threads,and their weight was weighed with an electronic balance.We designed four NaCl concen 鄄trations,0(control),100,200and 300mmol/L here.To reduce the sudden salt shock,NaCl concentration in 1/2Hoagland solution was increased by 100mmol/L every day to the designed concentration.Four repetitions were set in each treatment.The alligatorweed stems were vertically grown in 200ml culture flasks at greenhouse (25±3)℃-(35±3)℃.The stem length,node number,leaf number and fresh weight were recorded every three days,and the dry weight of the samples was measured at the end of the experiment as the fol 鄄lowing steps:took stems out of the cul 鄄ture flasks,absorbed the water on their surfaces with absorbent papers,and weighed their fresh weight immedi 鄄ately.Dried the fresh samples at 105℃for 20min,and then at 80℃to constant weight,and then to measure their dry weight.Statistical analysisWe used SPSS17.0software (SPSS Inc.,Chicago,IL,USA)for sta 鄄tistical analysis.Before that,Kol 鄄mogorov -Smirnov test and Levene ’s test were carried out on the data that we collected.The ratio of node num 鄄ber,leaf number,stem length,fresh weight,dry weight,ratio of dry weight to fresh weight and water content were analyzed through one 鄄way ANCOVA.The data were mean ±standard error.P <0.05means significant difference,and P <0.01means extremely signifi 鄄cant difference (two-tailed test).Results and AnalysisThe experimental results showed that the influence of NaCl on alligator 鄄weed at early stage was gradually in 鄄creased with NaCl concentration in 鄄creasing,and reached the peak at 300mmol/L,followed by at 200mmol/L,and was the minimum at 100mmol/L (Fig.1).Changes in node numberThe node numbers of the stems treated with 0,100and 200mmol/L NaCl increased;but that treated with 300mmol/L of NaCl did not change significantly.The ratios of node num 鄄ber=The node number measured on the 1st ,4th ,7th ,and 10th days/The node number measured on the 1st day.So the ratio of node number on the 1st day was 1.Throughout the whole experi 鄄ment process,the ratio of node num 鄄ber of the stems treated with 100and 200mmol/L of NaCl showed no signif 鄄icant difference with control;and that treated with 300mmol/L of NaCl showed no significant difference with the control on the 1st ,4th and 7th days,but significantly lower than the control on the 10th day [F (3,15)=4.488,P <0.05)],as shown in Fig.2.Changes in leaf numberThe leaf number on the stems treated with low concentrations of Na 鄄Cl (0and 100mmol/L)was generally increased over time,while some leaves fell occasionally.However,on the stems treated with high concentra 鄄tions of NaCl (200and 300mmol/L),some leaves wilted and the number of leaves decreased by the range which was increased with NaCl con 鄄centration increasing.The leaf num 鄄bers on the 4th [F (3,15)=10.812,P <0.01],7th [F (3,15)=19.242,P <0.001]and 10th [F (3,15)=28.849,P <0.001]days were extremely significantly different amongthe groups (Fig.3).Throughout the en 鄄tire experiment period,the leaf number on the stems treated by 100mmol/L of NaCl showed no significant difference with the control.The leaf number on the stems treated with 200mmol/L of NaCl was not significantly different on the 1st and 4th days,but significantly less than the control on the 7th and 10th days (P <0.01);and that treated with 300mmol/L of NaCl was significantly less than the control on the 4th ,7th and 10th days (P <0.01).13262013The different letters represent significant dif鄄ferences(P<0.05).Fig.4Stem length of alligatorweed treatedby different concentrations of NaClThe different letters represent significant dif鄄ferences(P<0.05).Fig.5Fresh weight of alligatorweed treatedby different concentrations of NaClFig.6Dry weight of alligatorweed treated bydifferent concentrations of NaClFig.7Ratio of dry weight to fresh weightand water content of alligatorweedtreated by different concentrations ofNaClChanges in stem lengthThe length of stems treated by0,100and200mmol/L of NaCl wasincreased by the range which de鄄creased with NaCl concentration in鄄creasing,but that treated with300mmol/L of NaCl was not significantlyincreased,it decreased firstly andthen slightly increased.The stemlength on the4th[F(3,15)=8.459,P<0.01],7th[F(3,15)=24.253,P<0.001]and10th[F(3,15)=38.267,P<0.001]days wereextremely significantly different amongthe groups treated by100,200and300mmol/L of NaCl(Fig.4).The lengthof stems treated with100mmol/L NaClshowed no significant difference withthe control on the1st and4th days,butwas significantly lower than the controlon the7th and10th days.The stemstreated with200and300mmol/L ofNaCl were extremely significantlyshorter than the control on the4th,7thand10th days(P<0.01).Changes in fresh weightThe fresh weight of stems treatedwith0,100and200mmol/L of NaClwas increased by the range whichdecreased with NaCl concentrationincreasing,but that treated with300mmol/L of NaCl was slightly de鄄creased.The fresh weight on the4th[F(3,15)=20.017,P<0.01],7th[F(3,15)=33.534,P<0.001]and10th[F(3,15)=28.09,P<0.001]days were extremely signifi鄄cantly different among the groupstreated by100,200and300mmol/L ofNaCl(Fig.5).The fresh weight ofstems treated with100,200and300mmol/L of NaCl was significantly lowerthan the control on the4th,7th and10thdays(P<0.01).Changes in dry weightNaCl treatment significantly influ鄄enced the dry weight of the stems[F(3,15)=13.294,P<0.001].As shown inFig.6,the fresh weight of the stemstreated with0,100,200and300mmol/L of NaCl decreased with NaClconcentration increasing,amongthem,that of stems treated with300mmol/L of NaCl was significantly lowerthan the control(P<0.001).The ratio of dry weight to freshweight and water contentNaCl treatment also significantlyinfluenced the ratio of dry weight tofresh weight,and the water content[F(3,15)=13.294,P<0.001].As shown inFig.7,the ratio of dry weight to freshweight of the stems treated by NaClwas increased while the water contentwas decreased with the increasingNaCl concentration.The ratio of dryweight to fresh weight of the stemstreated by300mmol/L of NaCl wassignificantly higher(P<0.01),while thewater content was significantly lowerthan the control(P<0.01).Conclusions and Discus鄄sionCompared with the control,100mmol/L of NaCl had no significant in鄄fluence on the ratio of node number,leaf number,dry weight,ratio of dryweight to fresh weight and water con鄄tent of alligatorweed,but significantlyinhibited the increase of stem lengthand fresh weight.200mmol/L of NaClexhibited no significant influence onthe ratio of node number,dry weight,ratio of dry weight to fresh weight andwater content of alligatorweed,but sig鄄nificantly inhibited the increase of itsleaf number,stem length and freshweight.The leaves on the stems treat鄄ed by200mmol/L of NaCl becameless,and significantly less than thecontrol on the7th and10th days.300mmol/L of NaCl significantly inhibitedthe increase of the ratio of node num鄄ber,leaf number,stem length,freshweight,and dry weight of alligator鄄weed,significantly reduced the watercontent,and increased the ratio of dryweight to fresh weight.The leaves onthe stems treated by300mmol/L ofNaCl became less,and were signifi鄄cantly less than the control on the4th,7th and10th days.In summary,the influence of NaCl onthe early growth of alligatorweed waspositively related to the NaCl concen鄄tration,and that of300mmol/L NaClwas most significant,followed by thatof200mmol/L,and was minimum at100mmol/L.Stem length and freshweight of alligatorweed were moresensitive to NaCl stress.In addition,the study also re鄄vealed that the node number,leafnumber,fresh weight and dry weight of(Continued on page1346)13272013Responsible editor:Qingqing YIN Responsible proofreader:Xiaoyan WUalligatorweed increased greatly undernormal circumstances during the shortterm of experiment (10d),suggestingthat alligatorweed is easy to propagate and grows fast,and thus is a good model plant in experiment.The ratio of dry weight to fresh weight of alligator 鄄weed increased while water contentdecreased with NaCl concentration in 鄄creasing,indicating that water ab 鄄sorption was inhibited at high concen 鄄trations of NaCl,which then affects a series of physiological and biochemi 鄄cal processes in alligatorweed.Thedecreased fresh weight and dry weightof alligatorweed revealed that the or 鄄ganics was synthesized slowly,whichwould seriously affect the growth of plant individuals .References[1]COHEN AN,CARLTON JT.Accelerat 鄄ing invasion rate in a highly invaded es 鄄tuary[J].Science,1998,279:555-558.[2]EHRENFELD JD.Ecosystem conse 鄄quences of biological invasions[J].An 鄄nual Reviews in 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盐胁迫对植物的影响及植物耐盐机理研究进展孟繁昊;王聪;徐寿军【期刊名称】《内蒙古民族大学学报(自然科学版)》【年(卷),期】2014(000)003【摘要】盐分是影响植物生长发育的一个重要环境因素,盐胁迫对植物的整个生命进程产生影响,盐分通过渗透胁迫、离子毒害,使植物细胞膜透性改变,造成氧化胁迫、代谢紊乱及蛋白质合成受阻等现象,植物的耐盐性主要体现在离子的选择性吸收和区域化作用、渗透调节、光合作用途径的改变以及活性氧清除机制。
%Salinity is one of the most important environmental factors affecting plant growth and development. Salt stress affects plant the whole life process. Salt makes the plant cell membrane permeability changes by osmotic stress, ion toxicity, cause oxidative stress, metabolism, protein synthesis of hindered and other phenomena. The mechanisms of salt resistance in plants were described from the ion selective absorption and regional effects, osmotic adjustment, changes of photosynthesis pathway and active oxygen scavenging mechanism.【总页数】4页(P315-318)【作者】孟繁昊;王聪;徐寿军【作者单位】内蒙古民族大学农学院,内蒙古通辽 028043;内蒙古民族大学农学院,内蒙古通辽 028043;内蒙古民族大学农学院,内蒙古通辽 028043【正文语种】中文【中图分类】Q945【相关文献】1.植物耐盐生理及耐盐机理研究进展 [J], 陈洁;林栖凤2.盐胁迫下芙蓉菊与4种菊属植物生理响应特征及其耐盐机理分析 [J], 林双冀;孙明3.盐胁迫对植物的影响及植物耐盐研究进展 [J], 彭程4.植物耐盐机理与耐盐植物选育研究进展 [J], 张建锋;李吉跃;宋玉民;邢尚军;郗金标;马丙尧5.盐分胁迫对植物的影响及植物耐盐机理研究进展 [J], 李彦;张英鹏;孙明;高弼模因版权原因,仅展示原文概要,查看原文内容请购买。
盐胁迫对植物光合作用影响的研究进展
张娟;姜闯道;平吉成
【期刊名称】《农业科学研究》
【年(卷),期】2008(029)003
【摘要】针对盐胁迫影响光合作用的几个重要方面进行了综述,主要包括盐胁迫对植物光合器官的形态结构、光合作用过程、抗氧化系统以及渗透调节物质等方面.研究表明,盐胁迫对光合作用的影响是由于不同的材料和实验方法,因此得出的结论也不同.为此盐胁迫对光系统Ⅱ(PSⅡ)的影响等问题还需进一步研究.
【总页数】7页(P74-80)
【作者】张娟;姜闯道;平吉成
【作者单位】宁夏大学,农学院,宁夏,银川,750021;中国科学院,植物研究所,北京,100093;中国科学院,植物研究所,北京,100093;宁夏大学,农学院,宁夏,银
川,750021
【正文语种】中文
【中图分类】S311
【相关文献】
1.重金属铅胁迫对植物光合作用影响的研究进展 [J], 李琦
2.盐胁迫下木本植物光合作用响应机制研究进展 [J], 张波;荣立苹;陈柳;曹飞
3.沉积物再悬浮对浅水湖泊沉水植物光合作用及生长影响的研究进展 [J], 谷娇;宁晓雨;靳辉;何虎;朱小龙;谈冰畅;李宽意
4.光照强度对园艺植物光合作用影响的研究进展 [J], 温博
5.盐胁迫下植物光合作用的研究进展 [J], 束胜;郭世荣;孙锦;袁颖辉;袁凌云
因版权原因,仅展示原文概要,查看原文内容请购买。
刘爱荣,张远兵,陈庆榆,张丛宇,熊周林,何庆元,王桂芹(安徽科技学院生命科学院,安徽蚌埠233100)摘要:用NaCl 浓度为0(对照)、50、100、150、200、250、300心莲子草[Alternanthera philoxeroides (Mart .)Griseb ]。
结果表明:盐胁迫下,植株鲜重、干重和根冠比都下降,含水量和肉质化程度先略高于对照,而后逐渐下降;根、茎和叶中Na +含量呈上升趋势,而K +含量呈下降趋势,且在同一浓度的盐处理下,叶的Na +和K +含量最高,茎的次之,根的最低;游离氨基酸(free amino acid ,FAA )逐渐减少,脯氨酸(proline ,Pro )含量先略低于对照,而后则急剧上升,可溶性糖含量(soluble sugar ,SS )的变化正相反;净光合速率(net photosynthetic rate ,Pn )、气孔导度(stomatal conductance ,G s )和蒸腾速率(transpiration rate ,Tr )呈下降趋势,胞间CO 2浓度(intercellular CO 2concentration ,Ci )呈上升趋势;膜稳定性呈下降趋势。
因此,空心莲子草是一种盐生植物,且最适盐浓度较低;Na +主要积累在地上部的茎和叶中。
推测它有可能向盐渍土壤蔓延。
关键词:空心莲子草;盐胁迫;生长;净光合速率;含水量;膜透性中图分类号:Q 945文献标识码:A 文章编号:0253-2700(2007)01-085-06LIU Ai -Rong ,ZHANG Yuan -Bing ,CHEN Qing -Yu ,ZHANG Cong -Yu ,XIONG Zhou -Lin ,HE Qing -Yuan ,WANG Gui -Qin(Life Science College ,Anhui Science and Technology University ,Benbu 233100,China )Alternanthera philoxeroides was treated with NaCl of different concentrations (0,50,100,150,200,250,300and The results showed :(1)Under NaCl treatment ,the fresh weight ,the dry weight and the root shoot ratio decreased .(2)Water content and the succulency degree of whole plant appeared to increase first and then gradually decline as salinity increased .(3)Na +contents increased and K +contents decreased in root ,stem and leaf .Na+and K+contents in leaf were the highest under the same salinity ,those in stem were next and those in root were thelowest .(4)FAA content gradually decreased .(5)Pro content appeared to decline first and then increase as salinity in -creased .(6)Soluble sugar changed in the opposite direction .(7)Pn ,G s and Tr decreased and Ci increased .The relative conductivity of leaves increased significantly .(8)The malondialdehyde (MD A )content declined at about NaCl at about NaCl 100-350Those parameters indicated that A .philoxeroides was halophyte ,but its salt tolerance was not strong and its NaCl optimal concentration lowed Na +mainly accu -mulated in stem and leaf of A .philoxeroide and it would probably spread to the salt soil .Alternanthera philoxeroides ;Salt stress ;Growth ;Photosynthesis ;Water content ;Membrane permeability空心莲子草(Alternanthe ra philoxeroides )为苋科、莲子草属的多年生宿根性草本植物,原产南美洲云南植物研究 2007,29(1):85~90Acta Botanica Yunnanica基金项目:安徽省教育厅自然科学研究项目(2005KJ 322z )和安徽科技学院引进人才项目(ZRC 200436)资助收稿日期:2006-03-28,2006-09-18接受发表作者简介:刘爱荣(1966-)女,副教授,硕士,从事植物抗性生理研究。
E -mail :arliu 88@tom .com的巴西、乌拉圭、阿根廷等国家(Coulson ,1976)。
我国自1958年作为牲畜饲料引入后,现已分布到四川、湖南等20多个省市(区)。
它适应性广,发生环境有草坪、池塘、沟渠、稻田、蔬菜田、旱作物田、果园、河流、房前屋后、路旁地边等,常常形成单一的优势群落,覆盖水域或陆地(王韧和王远,1988)。
其繁殖方式为典型的无性繁殖,地下异常生长的肉质根上能产生大量的不定芽,由不定芽产生许多植株。
适合水生和旱生,现已逃逸而成为极难防除的恶性杂草(娄远来等,2002)。
对农业、园艺、养殖业和交通运输业等造成了非常严重的后果(谭万忠,1994a ;刘全儒等,2002;张俊喜等,2004)。
目前,对空心莲子草的研究主要集中在其生物学特性(张格成,1993)、分布(谭万忠,1994b ;淮虎银等,2003)、不同生境的解剖结构(张彪,2001;娄远来等,2004;陶勇和江明喜,2004)、生物化学防除(王韧和王远,1988)、净化污水和利用(贾凤芝等,1998;夏汉平,2000)等方面。
而关于其耐盐生理机制鲜见报道。
本文对盐胁迫下空心莲子草植株鲜重、干重、含水量和肉质化程度,不同器官的Na +和K +含量,净光合速率和膜稳定性进行测定,旨在研究空心莲子草在盐渍环境下生长状况及耐盐能力,对于预测它在我国分布的潜在范围,防止其生态入侵和综合治理的方面提供参考。
1 材料与方法1.1 植物培养7月中旬剪取土壤质地均一,生长一致的空心莲子草植株(4节,苗长12cm )扦插于装有干净细砂的塑料盆中,每盆细砂量相等,每盆5株幼苗,共60盆。
用Hoagland 培养喷灌,室外培养。
幼苗生长至三周时进行NaCl 处理。
1.2 NaCl 处理NaCl 处理的预定浓度为0(对照)、50、100、150、200、250、300、Hoagland 营养液配置(+),为避免盐冲击效应,盐浓度高养液每天递增,直至预定浓度,然后每天定时、定量用预定盐浓度的营养液浇灌1次,浇灌量为持水量的2倍,液流出,从而将积余的盐冲洗掉,以保持NaCl 浓度恒定。
空心莲子草幼苗处理20d 后,分别取样测定有关指标,生长指标测定,每个处理6~8个重复;各项生理指标测定,每个处理至少3个重复,结果取平均值。
1.3 植株鲜重、干重、含水量、叶片肉质化程度及根冠比的测定将整株植物从培养盆中完整取出,用自来水快速冲洗干净,再用蒸馏水迅速将植株冲洗3次,用吸水纸吸干表面水分,立即称鲜重。
再将鲜样品材料置105℃烘箱中杀青10min ,转至65℃烘干,称得干重。
植物含水量=〔(单株鲜重-〕×100%;肉质化程度==1.4 Na +、K +、Pro 、FAA 和S S 含量测定参照Hunt (1982)方法,稍作改动。
取干样的根、茎和叶分别研磨,1mm 过筛,精确称取50mg 放于50mL 三角瓶中,加入25mL 0.盐酸,50℃振荡水浴45min ,过滤并用蒸馏水定容至50mL ,用WFX -110型原子吸收分光光度计分别测定Na +和K +含量。
各处理中,取同一部位新鲜材料,先后用自来水、蒸馏水清洗,用吸水纸吸干表面水分,用蒽酮比色法测定S S 含量(於新建,1999);用茚三酮比色法测定FAA 含量(西北农业大学,1985);采用茚三酮比色法Pro 含量测定(T roll ,1995)。
1.5 净光合速率、气孔导度、细胞间隙CO 2浓度和蒸腾速率的测定用CI -310便携式光合作用测定系统(美国CID 公司)于上午10:00~11:30测定净光合速率(Pn )。
测定时光合有效辐射为800μmol m -2s -1,温度为30℃,CO 22浓度为21%。
以上都取同一部位功能叶(从顶部倒数第5对)进行测定,各处理重复8~10次(n =8~10),结果取平均值。
1.6 质膜透性和MDA 含量测定叶片质膜透性按照李振国方法(1999)进行;MDA 含量测定按Heath and Packer 方法(1968)进行。
2 结果与分析2.1 植株鲜重、干重、含水量、肉质化程度和根冠比图1表明,盐胁迫下空心莲子草单株鲜重和干重均低于对照,且随着盐处理浓度的增加,单处理单株鲜重和干重分别比对照下降0.98%~64.5%和2.58%~55.3%。
盐胁迫对植物的伤害之一为水分胁单株含水量和肉质化程度均高于对照1.0%和1.6%(P >0.05);化程度分别比对照下降了0.5%~4.1%和2.8%~20.5%。
图1还显示,随着处理盐浓度的增加,根冠比逐渐下降,且下降幅度也逐渐增大。
2 云 南 植 物 研 究 29卷图1 盐处理对空心莲子草单株鲜重、干重、含水量、肉质化程度(a )和根冠比(b )的影响Fig .1 Effects of salt treatment on the fresh and dry weights ,the water content and succulency degree (a )and the root shoot ratio (b )of whole plant ofAlternanthera philoxeroides图2 盐处理对空心莲子草Na +(a )和K +(b )含量的影响Fig .2 Effects of salt treatment on Na +(a )and K +(b )contents of Alternanthera philoxeroides2.2 Na +和K +含量如图2所示:空心莲子草根、茎和叶Na +含量均随盐处理浓度增大而上升,而根、茎和叶K +含量则均随盐处理浓度增大而下降。