Flexcell FX-5000 ,细胞压力加载系统,Compression System

使用说明书为了您的安全请严格遵守使用说明使用本仪器要求完全理解并严格遵守与本仪器一起提供的使用说明.本仪器仅能用于本说明书中规定的用途.维修保养必须遵守本技术手册中所说明的维修保养时间和措施，以及“传感器使用说明”*中的规定。

仪器的修理只能由经过训练的维修人员进行。

配件不要使用定购信息中1)未列出的配件。

与电气仪器的安全连接对于使用说明或本技术手册中未提及的仪器，须咨询厂家或专家后方可进行电气连接。

在有爆炸危险的区域使用时的注意问题根据国家、欧洲或国际防爆规定进行过测试和批准的用于易爆区域的仪器或元件仅能按照批准中明确规定的条件使用，并要考虑相关的法律规定。

不得对设备或元件进行任何修改。

禁止使用有问题或不完整的零件。

在对这些仪器或元件进行修理时，要始终遵守相应的规定。

仪器的维修只能由经过训练的维修人员根据德尔格安全的维修说明书进行。

1) 技术手册，传感器数据列表／使用说明书和德尔格X-am 5000的电脑软件C C vision都包含在CD内。

2使用说明中的安全符号使用说明书中使用了一系列的警示使用仪器当中有可能产生危险的符号。

这些警示包括信号词汇警告您有可能碰到的危险的程度。

这些信号和相应的危险如下：危险：表明非常紧急的危险状况，如果不能够被避免，会导致死亡或者严重的伤害。

警告：表明一种潜在的危险状况，如果不能够被避免，会导致死亡或者严重的伤害。

小心：表明一种潜在的危险状况，如果不能够被避免，会导致中小程度的伤害。

注意：表明对相应危害的警告，不会导致人体伤害。

Indicates apotentially hazardous situation which,if not avoided, can result in minor or noderate injury.▲！CAUTION Indicates warnings relating to property damage, not resulting in bldily injury.NOTICE3设计用途便携式气体检测仪器可用于对工作区域和爆炸性危险区域内的环境空气中所含有的几种气体浓度进行连续的检测。

multi EA5000 操作规程(MMS5000)一、S(UVFD)和N(CLD)分析操作规程1. 开机（1） 打开Ar、O2 ，调节输入压力为0.4-0.6MPa。

（2） 打开S、N 检测器模块电源。

（3） 打开EA5000主机电源（4） 打开电脑及显示屏, 双击图标。

（5） 出现以下对话框，输入操作者姓名和密码。

如用默认操作者名字，则用户和密码都是User，点击“OK”。

（6） 再点击“Initialize analyzer”，软件进入初始化过程。

初始化之后，出现软件的工作区：（7）点击“activate method”按钮，选择所需要的方法：。

再点击“OK”，完成调用方法过程(如果已调好当前方法，此步可略)。

（8）等待系统状态都显示“OK”，并且已经显示注射器体积时，仪器已准备好所有测量条件。

（9）标准曲线的制作（全自动进样器）A．点击“Start calibration”按钮，如果没有相应的sequence，出现以下对话框，也可点击图标添加sequence，也出现以下对话框：点击“next”确认校正的方法，然后输入sequence的名字，也可以按照默认的名字，点击“next”后自动命名Calibration name，点击“next”，输入标样的个数，点击“next”确认，选择“ConstantOperands”（固定进样体积），点击“next”确认，如果有制备空白，在“measure”前面打钩，如果没有则不打钩，点击“next”确认，进样体积默认为你安装上的注射器体积，点击“next”，最后点击“OK”进入下一个界面。

B．当出现如下界面时，可进行标样点浓度的输入，分别点中相应的标样行，在界面的下半部分“Standard”菜单项下可进行浓度的输入，分别输入标样的浓度。

C ． 输入完毕后，点击标样行中的红叉后即可变成绿钩，如下所示：D．点击“OK”后，进入下面的测量界面：E．在确认对应自动进样器相应位置放置好标样以后，点击“Start measurement”开始测量，仪器会自动对每个标样进行测量。

©2018 QIAGEN Beverly, Inc. 100 Cummings Center Suite 407J Beverly, MA 01915 Quantabio brand products are manufactured by QIAGEN Beverly, Inc.Intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.95139 / IFU-095.1 REV 02PerfeC T a ® qPCR ToughMix ® UNG ROX ™DescriptionPerfeC T a qPCR ToughMix UNG ROX is a 2X concentrated ready-to-use reaction cocktail for PCR amplification of DNA templates that overcomes many known inhibitors of PCR often present in crude samples extracted from environmental specimens, plant tissues, or animal tissues. It is a versatile and robust real-time qPCR reagent that provides maximum sensitivity and PCR efficiency with a variety of fluorogenic probe chemistries, including TaqMan ® hydrolysis probes. PerfeC T a qPCR ToughMix UNG ROX contains all required reaction components, except primers, probe(s), and DNA template. Inclusion of uracil DNA glycosylase (UNG), and substitution of dTTP with dUTP, prevents amplification of carry-over contamination from previous dU-containing PCRs.A key component of PerfeC T a qPCR ToughMix UNG ROX is an ultra pure, highly processive thermostable DNA polymerase that is combined with high avidity monoclonal antibodies. This proprietary polymerase mix is highly resistant to PCR inhibitors and provides an extremely stringent automatic hot-start allowing reaction assembly, and temporary storage, at room temperature prior to PCR amplification. PerfeC T a qPCR ToughMix UNG ROX delivers exceptional performance with either fast or conventional PCR cycling protocols.Instrument CompatibilityDifferent real-time PCR systems employ different strategies for the normalization of fluorescent signals and correction of well-to-well optical variations. It is important to match the appropriate reference dye to each specific optical detection system. PerfeCta qPCR ToughMix, ROX contains an optimal concentration of a stabilized carboxy-X-rhodamine compound (ROX ™) for instruments that use an excitation wavelength of ~490 nm and 605 to 610 nm emission channel for the reference signal. Please consult our Product Finder selection tool at to find the correct product for your real-time PCR system. ComponentsPerfeC T a qPCR ToughMix UNG ROX (2X): 2X concentrated reaction buffer containing optimized concentrations of MgCl 2, dNTPs (dATP, dCTP, dGTP,dUTP), hot-start DNA polymerase, uracil DNA glycosylase (UNG), and stabilizers.Storage and StabilityStore components in a constant temperature freezer at -25°C to -15°C protected from light upon receipt. Repeated freezing and thawing does not impair product performance. After thawing, mix thoroughly before using.For lot specific expiry date, refer to package label, Certificate of Analysis or Product Specification Form.Guidelines for qPCR:▪ The design of highly specific primers and probes is a critical parameter for successful real-time PCR. The use of computer aided primer design programs is encouraged in order to minimize the potential for internal secondary stru cture and complementation at 3’-ends within each primer, the primer pair, and primer/probe combinations. For best results, amplicon size should be limited to 65 - 200 bp. Optimal results may require titration of primer concentration between 100 and 900 nM. A final concentration of 300 - 400 nM each primer and 100 to 250 nM probe is effective for most applications. Increasing the concentration of the primer that initiates synthesis of the target strand that is complementary to the probe can improve fluorescent signal for some primer/probe systems.▪ Preparation of a reaction cocktail is recommended to reduce pipetting errors and maximize assay precision. Assemble the reaction cocktail with all required components except sample template (genomic DNA or cDNA) and dispense equal aliquots into each reaction tube. Add the DNA template to each reaction as the final step. Addition of samples as 2 to 5- L volumes will improve assay precision.▪ Suggested input quantities of template are: cDNA corresponding to 1 pg to 100 ng of total RNA; 10 pg to 1 µg genomic DNA▪ After sealing each reaction, vortex gently to mix contents. Centrifuge briefly to collect components at the bottom of the reaction tube. Reaction AssemblyComponentVolume for 20-μL rxn.Final ConcentrationPerfeC T a qPCR ToughMix UNG ROX (2X) 10 µL 1xForward primer variable 100 – 900 nM Reverse primer variable 100 – 900 nM Probevariable 100 – 250 nMNuclease-free water variable Template2 – 5 µL variable Final Volume (μL)20 µLNote : For smaller or larger reaction volumes scale all components proportionally.Cat No. 95139-250 Size: 250 x 20-uL reactions (2 x 1.25 mL) Store at -25ºC to - 15°C protected from light95139-012 1250 x 20-µL reactions (10 x 1.25 mL)95139-05K5000 x 20-µL reactions (1 x 50 mL)©2018 QIAGEN Beverly, Inc. 100 Cummings Center Suite 407J Beverly, MA 01915 Quantabio brand products are manufactured by QIAGEN Beverly, Inc.Intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.95139 / IFU-095.1 REV 02PCR Cycling ProtocolFast 2-Step Cycling Fast 3-Step Cycling Standard Cycling UNG carry-over incubation – optional:Initial denaturation: PCR cycling (30-45 cycles):The appropriate step for fluorescent data collection varies for different probe assay formats. Data collection for 5’-nuclease probe assays (TaqMan probe) should be carried out at the end of the extension step. Use the annealing step for data collection with hybridization probe assays (HybProbe ® FRET hybridization probes, Molecular Beacons, Solaris ® qPCR Assays, Scorpions ® primers, etc.).End-point analysis should be carried out at a suitable temperature for your detection probe chemistry.‡ UNG incubation is optional. Alternate protocols are acceptable. We find that a 5 minute incubation at 45ºC is significantly more effective at eliminating carry-over contamination than the more typical procedure of 50ºC for 2 min.* Full activation of the DNA polymerase occurs within 10 seconds at 95ºC; however, optimal initial denaturation time is template dependent and will affect qPCR efficiency and sensitivity. Amplification of genomic DNA or supercoiled plasmid DNA targets may require 5 to 10 min at 95ºC to fully denature and fragment the template. Short double-stranded DNA template (PCR product) or single-stranded DNA template, such as cDNA, may require as little as 1s at 95ºC. Use 30s at 95ºC as a general starting point.† Extension time is dependent upon amplicon length and the minimal data collection time requirement for your qPCR instrument. Use 30s at 60ºC as a general starting point. Some assay designs and/or detection chemistries may require a 3-step cycling protocol for optimal performance. Optimal annealing temperature and time may need to be empirically determined for any given primer set and real-time instrument.Quality ControlKit components are free of contaminating DNase and RNase. PerfeC T a qPCR ToughMix UNG ROX is functionally tested in qPCR. Kinetic analysis must demonstrate linear resolution over six orders of dynamic range (R 2 > 0.990) with a 2-fold discrimination of starting template and a PCR efficiency > 95%.Limited Label LicensesUse of this product signifies the agreement of any purchaser or user of the product to the following terms:1. The product may be used solely in accordance with the protocols provided with the product and this manual and for use with components contained in the kit only. QIAGEN Beverly, Inc. grants no license under any of its intellectual property to use or incorporated the enclosed components of this kit with any components not included within this kit except as described in the protocols provided with the product, this manual, and additional protocols available at Some of these additional protocols have been provided by Quantabio Product users for Quantabio users. These protocols have not been thoroughly tested or optimized by QIAGEN Beverly, Inc.. QIAGEN Beverly, Inc. neither guarantees them nor warrants that they do not infringe the rights of third-parties.2. Other than expressly stated licenses, QIAGEN Beverly, Inc. makes no warranty that this kit and/or its use(s) do not infringe the rights of third-parties.3. This kit and its components are licensed for one-time use and may not be reused, refurbished, or resold.4. QIAGEN Beverly, Inc. Specifically disclaims any other licenses, expressed or implied other than those expressly stated.5. The purchaser and user of the kit agree not to take or permit anyone else to take any steps that could lead to or facilitate any acts prohibited above. QIAGEN Beverly, Inc. may enforce the prohibitions of this Limited License Agreement in any Court, and shall recover all its investigative and Court costs, including attorney fees, in any action to enforce this Limited License Agreement or any of its intellectual property rights relating to the kit and/or its components.This product is licensed under U.S. Patent No. 7,972,828 and corresponding US and foreign patents and patent applications for any use for research and development purposes; the license expressly excludes any use for diagnostic testing or clinical therapeutics in humans or animals.The use of this product is covered by at least one claim of U.S. Patent No. 7,687,247 owned by Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The buyer cannot sell or otherwise transfer (a) this product, (b) its components, or (c) materials made by the employment of this product or its components to a third party or otherwise use this product or its components or materials made by the employment of this product or its components for Commercial Purposes. Commercial Purposes means any activity for which a party receives or is due to receive consideration and may include, but is not limited to: (1) use of the product or its components in manufacturing; (2) use of the product or its components to provide a service, information, or data; (3) use of the product or its components for therapeutic, diagnostic or prophylactic purposes; or (4) resale of the product or its components, whether or not such product or its components are resold for use in research. The buyer cannot use this product or its components or materials made using this product or its components for therapeutic, diagnostic or prophylactic purposes. Further information on purchasing licenses under the above patents may be obtained by contacting the Licensing Department, Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008. Email: *************************.PerfeC T a, and ToughMix are registered trademarks of QIAGEN Beverly, Inc.. TaqMan is a registered trademark of Roche Molecular Systems, Inc. HybProbe is a registered trademark of Roche Diagnostics GmbH. ROX is a trademark Life Technologies Corporation. Solaris is a registered trademark of Thermo Fisher Scientific Inc. Scorpions is a registered trademark of DxS, Ltd. of Manchester, UK.。

深研生物cellsep pro 参数-回复深研生物是一家专注于生物科技领域的公司，他们最近推出了一款名为CellSep Pro的生物技术产品。

在这篇文章中，我们将一步一步地回答关于CellSep Pro的参数和特点。

首先，让我们来了解一下CellSep Pro的基本参数。

CellSep Pro是一种离心技术仪器，用于分离和分析细胞群。

它具有高效、精确的离心技术，可广泛应用于细胞生物学、遗传学和药物研发等领域。

CellSep Pro具有以下主要参数：1. 离心力和转速控制：CellSep Pro具有可调节的离心力和转速参数，可以根据用户需求进行调整。

这使得它可以适应不同类型细胞的分离要求，从而提高实验的准确性和可靠性。

2. 容量和管座：CellSep Pro提供不同容量的离心管座和离心桶，以适应不同规模的细胞分离需求。

这为用户提供了更多样化的选项，可以同时处理多个样本，提高工作效率。

3. 温度控制功能：CellSep Pro配备了温度控制系统，可以精确地控制离心过程中的温度。

这对于需要在特定温度下进行的实验非常重要，能够保证实验结果的准确性和可重复性。

接下来，让我们来看一下CellSep Pro的特点和优势。

CellSep Pro具有以下几个显著的特点和优势：1. 高分辨率和高效率：CellSep Pro采用先进的离心技术，能够精确地分离和分析细胞群。

它可以有效地分离不同种类的细胞，并提供高质量的分离结果。

2. 用户友好的界面：CellSep Pro具有简洁直观的用户界面，使操作更加方便和容易。

使用者只需要简单设置参数，并通过触摸屏或计算机界面控制离心过程。

3. 安全和可靠性：CellSep Pro具有多重安全保护机制，如超速保护、温度保护和故障诊断等，确保实验过程的安全和可靠性。

这使得用户可以放心地使用该仪器进行实验。

4. 可扩展性：CellSep Pro具有良好的可扩展性，可以与其他实验室仪器和数据分析系统进行接口连接。

中科大FPLC使用参考1.2 FPLC使用指南From : USTC | Date : 2016-12-01简介（INTRODUCTION）FPLC全称为快速蛋白液相色谱（Fast protein liquid chromatography），其原理与高效液相色谱理论类似，是由经典的液体柱层析引入气相色谱理论，并且对相体进行了改革，配用高压输液泵，采用高灵敏检测器、梯度洗脱装置、自动收集装置和微机等发展起来的现代液相色谱。

我们实验室有两套纯化仪，一台是AKTA prime plus（着重介绍），另外一台是AKTA pure protein(简单介绍）。

AKTA Pure 纯化系统（详见AKTA Pure onsite-training）AKTA Prime Plus 纯化系统安全 (Safety)组件 (Components)方法编程 (Method programming)使用方法 (Usage)维护注意事项 (Maintenance)安全(Safety)1. 警告！系统必须与接地电源插座连接。

2. 警告！用户不能打开系统，因为系统含高压电路，会造成致命的电击。

3. 警告！必须总有上样环连接在上样阀的接口2和6。

这是为了在转换此阀时防止液体从这些接口喷出。

尤其是使用危险化学品时，这是特别危险的。

4. 警告！如果有大量溢出的液体渗入系统外壳并同带电部件接触的危险，应立即关闭系统并同指定的技术服务人员联系。

5. 警告！NaOH有害健康，应避免泄漏。

组件（Components）AKTA prime plus蛋白质纯化系统包括下列部件：1. 缓冲液阀和梯度转换阀(Buffer valve and gradient switch valve)：缓冲液阀用于选择使用缓冲溶液，用于系统泵施加大的样品体积。

梯度转换阀用于建立梯度。

2. 系统泵 (System pump)：系统泵用于经系统运送液体，如样品或缓冲溶液。