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第1篇一、产品概述本试剂盒是针对多糖含量测定而设计的高效、便捷的检测工具。
适用于各类生物样本中多糖含量的定量分析，包括但不限于植物提取物、动物组织、微生物发酵液等。
本试剂盒采用酶联免疫吸附法（ELISA）原理，通过特异性抗体与多糖的结合，实现对多糖含量的精确测定。
二、产品组成1. 试剂盒- 多糖抗体- 标准品- 酶标抗体- 底物溶液- 洗涤缓冲液- 封闭液- 终止液2. 仪器- 酶标仪- 微量移液器- 恒温水浴箱- 试管3. 试剂- 酶标仪专用缓冲液- 酶标仪专用洗涤液三、使用方法1. 样本准备- 将待测样本按照说明书要求进行稀释。
- 确保样本无污染，避免酶标仪读取误差。
2. 加样- 在酶标板孔中加入50μl的标准品或样本稀释液。
- 在所有孔中加入50μl的多糖抗体。
3. 孵育- 将酶标板放入恒温水浴箱，37℃孵育1小时。
4. 洗涤- 使用酶标仪专用洗涤液，将酶标板充分洗涤3次。
5. 加酶标抗体- 在所有孔中加入50μl的酶标抗体。
- 37℃孵育1小时。
6. 洗涤- 使用酶标仪专用洗涤液，将酶标板充分洗涤3次。
7. 加底物溶液- 在所有孔中加入50μl的底物溶液。
- 避光孵育15分钟。
8. 终止- 在所有孔中加入50μl的终止液。
9. 检测- 使用酶标仪在450nm波长下检测各孔的吸光度（OD值）。
10. 数据分析- 将测得的OD值与标准曲线进行比较，计算多糖含量。
四、注意事项1. 试剂和仪器- 使用前请仔细阅读说明书，确保试剂和仪器符合使用要求。
- 使用酶标仪时，请按照仪器说明书进行操作。
2. 样本处理- 样本处理过程中，请确保无污染，避免影响检测结果。
- 样本稀释时，请使用适宜的稀释液，确保稀释倍数合理。
3. 操作步骤- 操作过程中，请严格按照说明书进行，避免人为误差。
- 注意操作环境，避免交叉污染。
4. 标准曲线- 标准曲线的绘制应使用新鲜标准品，并确保标准曲线线性良好。
5. 质量控制- 定期进行质量控制，确保试剂盒的准确性和可靠性。
1Warning!EN61326-1EN60825-11. Safety notices Before using the thermodetector, please read the manual carefully. Do not use any solvent to clean the thermodetector. Safety symbols:Important information prompt for dangerComplies with European CE safety specificationThe instrument complies with the following standards:Do not align the laser to human eyes or reflective surface2. Notes When the ambient temperature changes in a sudden, it is required to place the thermodetector in the environment for 30 minutes , and measure when internal and external temperatures of the thermodetector coincide.Try to avoid any electromagnetic field caused by electric welding and induction heating.Do not place the thermodetector close to or on a high temperature object.Keep the thermodetector clean, and avoid dust from entering the tube.23. Appearance description1. Alarm indicator2. Liquid crystal display3. Laser control key/digital turn down key ▼4. Mode key5. Back-light/digital turn up key ▲6. Infrared sensor induction zone7. Laser indicator8. Measurement trigger 9. Battery cover4. Liquid crystal display description1: Primary display: Displays measured temperature.2: Function indication: MAX (Maximum value)3: Displays the maximum value4: Fahrenheit degree5: Centigrade degree6: Low voltage indication7: Laser indication8: Measurement indication9: Low alarming10: High alarming11: Data hold12: Radiance indication35. Measurement methods1. Set the upper limit of the instrument alarm:Press and hold the Mode key for 2 seconds, to enter instrument setting, and press MODE key to shift to alarm upper limit setting, in this case, Hi is displayed in the instrument function indication zone, and the alarm upper limit is displayed in the zone. Press ▲/▼ key to increase or decrease the alarm value, and long press ▲/▼ key to accelerate the increase or decrease of the set value.2: Set the low alarm value of the instrumentPress and hold the Mode key for 2 seconds, to enter instrument setting, and press MODE key to shift to alarm lower limit setting, in this case, Low is displayed in the instrument function indication zone, and the alarm lower limit is displayed in the zone. Press ▲/▼ key to increase or decrease the alarm value, and long press ▲/▼ key to accelerate the increase or decrease of the set value.3: Set the instrument radiancePress and hold the Mode key for 2 seconds, to enter the instrument setting, and press the MODE key to shift to the instrument radiance setting, in this case, the instrument radiance indication zone flashes. Press the ▲/▼ key to increase or decrease the radiation value, and long press the ▲/▼ key to accelerate the increase or decrease of the set value.44: Set the instrument temperature unitPress and hold the MODE key for 2 seconds, to enter the instrument setting, and press the MODE key to shift to the instrument temperature measurement unit, the unit symbol on the display flashes, and press the ▲/▼ key to change the unit symbol.5: Exit the settingPress the trigger or long press the MODE key, to exit the instrument setting.6: Turn on/off laserPress the key to turn on or off laser, and the instrument will display the laser symbol7:Turn on or off back-lightPress the key to turn on or off back-light.8: Non-contact temperature measurementAim the thermodetector at the object, and hold the trigger, to conduct continuous measurement of temperature. After displaying stably, release the trigger, and the5When holding the trigger, the secondary display of the instrument will display the maximum value of the measured temperature.When the measured value is greater than the upper limit of high alarm or the measured value is less than the lower limit of low alarm, the red alarm indicator will turn on to alarm.6. Target distance ratio (D:S ratio)The thermodetector has a certain visual angle and visual field, as shown in the following figure.The measured target Tube Light-sensitive componentIn order to guarantee the measured object fills in the visual field of the thermodetector, which means the thermodetector only "sees" the measured object rather than other objects. Larger objects may cause larger temperature measurement distances; for smaller objects, the measurement distances must be close. The ratio of measurement distance to the measured target (D:S) is 12:1, as shown in the following figure:677. RadianceThe radiance characterizes the ability of an object to radiate infrared ray. Larger radiance will lead to stronger radiation ability on the object surface.Radiance of the majority of organic matters or metal oxidized surfaces ranges between 0.85 and 0.98. The radiance of the thermodetector is 0.95 by default. During measurement, set the radiance of the instrument the same with the radiance of the measured object. During measurement, please pay attention to the impact of emissivity on measurement results.The following table is the radiance reference table.Measured surfaceRadiance Aluminum Oxidized0.2~0.4A3003alloy (oxidized)0.3A3003alloy (coarse)0.1~0.3Brass Polishing0.3Oxidized0.5 Copper Oxidized0.4~0.80.6Electrical terminalboardHastelloy0.3~0.8 Ferro-nickel Oxidized0.7~0.95Abrasive blasting0.3~0.6Electropolishing0.15Iron Oxidized0.5~0.9Rust0.5~0.7 Iron(casting)Oxidized0.6~0.95Unoxidized0.2Fusion cast0.2~0.3 Iron(casting)passivation0.9Lead Coarse0.4Oxidized0.2~0.6 Molybdenum oxidation0.2~0.68Nickel oxidation0.2~0.5 Platinum black0.9 Steel Cold rolling0.7~0.9Grinding steel plate0.4~0.6Polished steel plate0.1 ZincOxidized0.1 Asbestos0.95 Asphalt0.95 Basalt0.7 Carbon(unoxidized)0.8~0.9 Graphite0.7~0.8 Silicon carbide0.9 Ceramics0.95 Clay0.95 Concrete0.95 Cloth0.95 Glass plate0.85 Gravel0.9519LCD displayColor LCD display D:S12：18. Replacement of battery When battery is low, the battery symbol will light up, in this case, it is required to replace the battery. Open the battery cover with your hands, a nd replace with a new1.5Vx2AAA battery. Refer to the following figure:9.Technical indexes。
AND The next generation Digital Burette!I High accuracyPrecise titration within the errorlimits of Class AI Smooth, low-force operationFor sensitive, drop-wisetitrationI Compact designLight and stableI Easy handlingNo switching between 'fill'and 'titrate'I Simple maintenanceCleaning and part replacementright in the labI PC interface (optional)Data transmission directly tothe PCWith the Titrette® bottle-topburette, available in 10 ml,25 ml, and 50 ml sizes, youcan titrate quickly and reliablywith highest precision, even inclose quarters, with no powerhookup needed – in the lab, inproduction, or in the field!class A precision Titrette®A closer lookThe control elements of the Titrette® bottle-top burette havean intuitive layout. Separate buttons for On/Off and Pause;Clear button allows user to reset the display and selectfunctions. Easy-grip hand wheel action and smoothprecision gears for fast or drop-wise titration makehandling extremely simple and reliable.CLEAR/Select buttonDigital displayPause button On/Off buttonPistonDispensing cylinderTitration andrecirculating valve Inspection windowValve blockFreely rotating(Bottle thread GL 45) Titrating tubevertically adjustable,with integrateddischarge valveScrew capTelescoping filling tubeRecirculating tubePC interface (optional) (see ordering information)Replaceable batteriesHand wheelsAdjustment with Easy CalibrationWith Easy Calibration technology, you can easily make calibra-tion adjustments of the instrument 'at the push of a button'. No tools needed! An ad-justment may be neces-sary if the instrument has been in use for a longer period or if parts arereplaced. To show that an adjustment has been carried out, a small 'CAL' icon will be shown in the upper portion of the display.Changing decimal place settingsFor use as a micro-burette, you can switchthe titrated volume display from 2 to 3 decimal places under 'dP' (decimal point).Above 20.00 ml, the display automa-tically switches to 2 decimal places.Calibration scheduleTo save a date for the next calibration, simply store it under 'GLP'. The date can be called up each time the instru-ment is turned on. Hold the on/off button down for a little longer, and the letters 'GLP', along with the month and year of the calibration date, are shown.Save power with Auto Power OffThe instrument switches off automatically after longer periods of inactivity. The current display value is stored, and returned to the display after the power is switched on again manually. Under 'APO' (Auto Power Off), you can set the time until automatic power-offfrom 1 to 30 minutes.Four additional helpful electronic functions make your work easier. Hold down the CLEAR button to select the desired function:ExtrasNo switching needed!To change between filling and titration, no switching is needed. The instrument automatically detects whether you are filling or titrating by the direction of hand wheel rotation greatly reducing the risk of handling errors. Pressing the Pause but-ton allows you to interrupt the titration if priming was incom-plete. Simply push the button again to resume titration.Class A precision!The Titrette ® bottle-top burette has an innovative new mea-suring system which matches the Class A accuracy of glass burettes and, if needed, can offer the precision of 3 decimal places below 20 ml. This is an important feature for users who need to work within the Class A error limits of glass burettes according to DIN EN ISO 385 (e.g., in pharmaceutical work).Drop by drop!The large, easy-grip hand wheels are conveniently easy to turn. With the optimized gear ratio, you can fill the device quickly and titrate drop-wise with great sensitivity.The drop size for the 10 ml in-strument is approx. 20 µl, and for the 25 and 50 ml instru-ments approx. 30 µl.With the Titrette ® bottle-top burette, you can titrate drop-wise with great sensitivity. No switching needed between filling and titrating! The instrument is light-weight, compact, and can be dismantled for cleaning and maintenance in the lab. Easy handling!Easy HandlingLight-weight and compact!All components move within the housing, reducing headroom requirements. The compact design and the light weight ensure good stability. The titrating tube can be adjusted horizontally and vertically. This provides flexibility when posi-tioning the instrument, e.g., when using a magnetic stirrer or different bottle sizes.User seviceable!We have designed the instru-ment to be disassembledquickly and easily – in a matter of minutes – for cleaning, to replace the piston/cylinder, or to replace the batteries.Now you can carry out mainte-nance conveniently and easily in the lab, and in a few minutes the instrument is ready to go again!Light protection!For protection of light-sensitive media, you can replace the clear inspection windows with the amber colored windows (included). It's quick and easy, because the windows just clip into place.Easy HandlingComparison of error limits* Error limits related to the nominal capacity (= maximum volume) indicated on the instrument, obtained when instrument anddistilled water are equilibrated at ambient temperature (20 °C/68 °F) and with smooth operation.** Error limit: EL = A + 2CV, according to DIN EN ISO 8655-6 Annex B(A = accuracy, CV = coefficient of variation, EL = error limit)Operating limitsThis instrument is designed for titrating liquids,observing the following physical limits:I +15 °C to +40 °C (59 °F to 104 °F)of instrument and reagentI Vapor pressure up to 500 mbarI Viscosity up to 500 mm2/sI Altitude: maximum 3000 m above sea levelI Relative humidity: 20% to 90%Limitations of useChlorinated and fluorinated hydrocarbons orchemical combinations which form depositsmay make the piston difficult to move or maycause jamming.Compatibility of the instrument for a specialapplication (e.g., trace material analysis) mustbe checked by the user. For additional infor-mation, please contact the manufacturer.The instrument is not autoclavable!The instrument can be used for the following titration media(maximum concentration 1 mol/l):When the instrument is properly handled, dispensed liquid will only come into contact with thefollowing chemically resistant materials: borosilicate glass, Al2O3, ETFE, PFA, FEP, PTFE,platinum-iridium; PP (screw cap).Material and reagentsNote:If you need an official certification which confirms theerror limits that are much stricter than those of DIN ENISO 8655-3, we recommend a calibration certificatefrom an accredited calibration laboratory (e.g., theDAkkS laboratory at BRAND). Technical dataAcetic acidAlcoholic potassium hydroxidesolutionAmmonium iron (II) sulfate solutionAmmonium thiocyanate solutionBarium chloride solutionBromide bromate solutionCerium (IV) sulfate solutionEDTA solutionHydrochloric acidHydrochloric acid in AcetoneIodide Iodate solution*Iodine solution*Iron (II) sulfate solutionNitric acidOxalic acid solutionPerchloric acidPerchloric acid in glacial aceticacidPotassium bromate solutionPotassium bromide bromatesolutionPotassium dichromate solutionPotassium hydroxide solutionPotassium iodate solutionPotassium permanganate solution*Potassium thiocyanate solutionSilver nitrate solution*Sodium arsenite solutionSodium carbonate solutionSodium chloride solutionSodium hydroxide solutionSodium nitrite solutionSodium thiosulfate solutionSulfuric acidTetra-n-butylammoniumhydroxide solutionTriethanolamine in Acetone*Zinc sulfate solutionThe titration volume is displayed in steps of 1 µl at instruments with 10 mland 25 ml size and in steps of 2 µl for 50 ml size instruments. For titrationvolumes above 20 ml the display will automatically switch to steps of 10 µl.* Use light shield inspection windowOrdering informationTitrette ®Items supplied:Titrette ® bottle-top burette, conformity certified, performance certificate, telescoping filling tube (170 - 330 mm), recirculation tube, 2 batteries (AAA/UM4/LR03), 3 PP bottle adapters (GL 45/32, GL 45/S 40, GL 32/NS 29/32), 2 colored light shield inspection windows, operating manual.Storage conditionsStore the instrument and accessories at dry conditions.Storage temperature: -20 °C to +50 °C Relative air humidity: 5% to 95%* Additionally included:connector cable (Sub-D plug connector, 9-pin), one CD (driver software and open RS232 communication protocol). The CD also includes an example application in XLS-file format, as well as a special operating manual.The instrument is available with an optional RS 232 communica-tions interface.Advantages compared to the standard configuration:I The titration results are automatically transmitted to the PC by double-clicking on the CLEAR key. This eliminates transcript- ion errors while recording primary data, and complies with an important requirement of GLP.I With each data transfer, the burette sends the titrated volume, the serial number of the instrument, the nominal volume and the adjustment value, as well as the next scheduled calibration date. Thus, all raw data is collected and displayed together with actual date/time stamp from the PC.The transmitted data is recognized as keyboard inputs by the PC. This universal input format ensures that the instrument is compatible with all PC applications that accept keyboard inputs.To connect the instrument to a USB interface, simply use a standard USB/RS 232 adapter.PC interface (optional)Note:When ordering devices with DKD certificates, the prefix "DKD" must be added to the order number, e.g., DKD 4760 161.BRAND GMBH + CO KG · P.O. Box 1155 · 97861 Wertheim · GermanyTel.:+499342808-0·Fax:+499342808-98000·E-Mail:*************·Internet:www.brand.de9943 55 · P r i n t e d i n G e r m a n y · 13/0813Titrette ® and BRAND ® are trademarks of BRAND GMBH + CO KG, Germany.Our technical literature is intended to inform and advise our customers. However, the validity of general empirical values, and of results obtained under test conditions, for specific applications depends on many factors beyond our control. Please appreciate, therefore, that no claims can be derived from our advice. The user is responsible for checking the appropriateness of the product for any particular application.Subject to technical modification without notice. Errors excepted.Accessories · Spare partsInspection window1 set colorless and 1 set amber colored (light shield).Filling valvewith olive-shaped nozzle and sealing ring. Pack of 1.Telescoping filling tube FEP.Pack of 1.Cat. No.6783Cat. No.6636Cat. No.7042 04Cat. No.7042 05170 - 330 mm250 - 480 mmTitrating tubewith screw cap and integrated discharge and recirculationvalve. Pack of 1.for volume ml Cat. No.* M anufactured from Jan. 2012 onwards (serial number 01K)PistonPack of 1.for volume ml Cat. No.Dispensing cylinder with valve blockPack of 1.for volume ml Cat. No.* M anufactured from Jan. 2012 onwards (serial number 01K)Bottle StandPP. Full plastic material. Support rod 325 mm, base plate 220 x 160 mm, weight 1130 g. Pack of 1.Cat. No.7042 75Drying tubeDrying tube and seal, without drying agent. Pack of 1.Cat. No.7079 30For laboratory bottles, and an assortment of adapters, please visit www.brand.de.。
Thank you for choosing the RIGHTEST ELSA Blood Glucose Monitoring System. Please read this manual thoroughly before testing. It provides all the information you need to use your product.The RIGHTEST ELSA Blood Glucose Meter must only be used with RIGHTEST ELSA Blood Glucose T est Strips and Control Solution GC570. Use of other test strips or control solutions may lead to incorrect results.Please only purchase test strips in the same country as which you purchased your meter. Use of test strips from different countries may result in inaccurate test results.You are recommended to monitor blood glucose regularly. The RIGHTEST ELSA Blood Glucose Monitoring System is accurate and easy-to-use making it your reliable diabetes management assistant.The RIGHTEST ELSA Blood Glucose Monitoring System is manufactured and supported by Bionime Corporation. If you have any questions or concerns, please contact Bionime Customer Service or*****************************.The RIGHTEST ELSA Blood Glucose Monitoring System is designed for in vitro diagnostic (fortesting outside the body) use only and can be used by home user and healthcare professional.The system can test glucose concentration in fresh capillary whole blood (drawn from fingertip, palmand forearm).The glucose result displayed is calibrated into the plasma glucose testing equivalent.The system is not intended for screening or diagnoses of diabetes mellitus.Preface3 2Intended Use76Table of ContentsTable of Contents3031About RIGHTEST Blood Glucose Monitoring SystemContents of the RIGHTEST Blood Glucose Monitoring SystemRIGHTEST MeterRIGHTEST T est Strip Preparing for a TestActivating the Meter and Changing the BatterySetting Up Your Meter - Setting the Time, Date, Volume and T est UnitT urning the Meter On/Off Testing ProcedurePerforming a Blood Glucose T estAlternative Site T esting (AST)Viewing Window Appearance81014161822232729Quality Control " Lo " and " Hi " Readings Quality Control T est Reviewing Stored ResultsPerforming a Quality Control T est Recalling Results and Viewing the Average Result Product MaintenanceError Messages and TroubleshootingSpecification Limitations Customer Service Warranty 3842454748493335Product MaintenanceIndirect transmission of Human Immunodeficiency Virus (HIV), Hepatitis B Virus (HBV) and Hepatitis C Virus (HCV) during the delivery of healthcare services has been increasingly reported. Persons using blood glucose monitoring systems have been identified as one risk group due to sharing of lancets, lancing devices, and blood glucose meters.The cleaning procedure is to remove dust, blood and body fluids from the surfaces and should be performed whenever the meter or lancing device is visibly dirty. The disinfecting procedure is necessary to kill pathogens such as HIV, HBV and HCV on the device.NOTE: the cleaning procedure can only remove visible contaminates from surfaces. Only the disinfecting procedure can eliminate non-visible pathogens.If the meter is being operated by a second person who is providing testing assistance to the user, the meter and lancing device should be decontaminated prior to use by the second person.The following disinfecting wipe has been tested and may be used to clean and disinfect the meter and lancing device:Caviwipes Disinfecting T owelettes, manufactured by Metrex. Uses Isopropanol as the active ingredient and has been shown to be safe for use with the meter and lancing device.MaintainingKeep your meter and test strip free of dust, water or any other liquid. Store the meter in the carrying case when not in use. If your meter is dropped or damaged, perform a quality control test with the control solution before performing a blood glucose test.Product Maintenance3938SpecificationMeter Battery LifeMeter DimensionsMeter WeightMonitorDisplay AreaStorage / Transportation ConditionsSampleMinimum Sample VolumeHematocritT est Strip Storage / Transportation Conditions Approx. 1,000 tests95.0 mm x 43.8 mm x 13.0 mm43.0 ± 5 g (with battery)LCD display29.0 mm x 38.0 mm10 - 40˚C (50 - 104˚F), 10 - 90% RHRefer to RIGHTEST ELSA Strip insertLimitations- RIGHTEST ELSA Blood Glucose Monitoring System is not intended for serum or plasma test.- Inaccurate test results may be obtained at high altitude more than about 3,048 meters (10,000 feet)above sea level.- T est results may be falsely low if the patient is severely dehydrated, in shock, or in a hyperosmolarstate (with or without ketosis).- Patients going through oxygen therapy may yield falsely low results.- These test strips should not beused with meters to test critically ill patients or neonates.- The blood glucose test result may be affected by high blood concentration of interferenceingredients,if you need more detail information about interference ingredients, please see Blood Glucose ELSAT est Strip Insert.- Alternative site testing (AST) should only be performed during steady-state times (when glucose isnotchanging rapidly).- Please refer to the (Alternative site testing (AST)) chapter if you want to test on palm or forearm.- DO NOT test on alternative site (palm, forearm) if you are testing for insulin dose calculations orforContinuous Glucose Monitoring (CGM) devices calibration, or hypoglycemia (Low blood glucose).- T o avoid potential electromagnetic or other interference, keep meter away fromelectromagneticradiation sources such as X-ray or MRI.- Keep your meter away from dust, water orany liquid.4746。
ATAGO糖度计参数说明ATAGO糖度计技术参数说明PAL系列迷你数显折射计仅手掌大小，经济实用、操作简便。
注重产品的洁净设计，通过减少非必需附件而减少细菌可能繁殖的环境，并具备完全流水冲洗的性能，适应各行业的卫生要求。
符合HACCP标准。
同时，具备E.L.I.(外部光线阻止)功能，保证室外测量的精度。
·防水防尘性能IP65·E.L.I.(外部光线阻止)功能·清水归零，操作简便·小巧轻便，仅重100g·自动温度补偿功能·设计简洁，可单手操作·配便携式挂钩，便于携带手持式糖度计PR-α系列·精度提高至Brix±0.1%，可同时测量果汁、食品、饮料等的糖度及化工液体浓度如：切削油、清洗液、不冻液等·可设置客户专用标度，客户自己预设系数(浓度=Brix X系数)就可直接读出此样品浓度·采用E.L.I.,可防止强光下的误测量，保证稳定的测量结果·清水较零，自动温度补偿·响应时间3秒·样品体积0.1ml·防护等级IP64自动折射仪 RX-5000αRX-5000α( alpha ) 是能够内部设定测量温度的自动折射仪，能够快速地测量折射指数、糖度或各式液体的浓度，以下为本产品的特性：因为RX-5000α ( alpha ) 具有电热模块以控制温度，所以不需要恒温水箱。
在样本达到目标温度之后，测量会自动开始。
在目标温度下，折射指数与糖度会快速显示可取得高糖度± 0.03% 与折射指数± 0.00004 准确度。
RX-5000α ( alpha ) 会显示您所设的控制范围的高低界线。
如果测量值与您的标准液体值或其它折射仪测量的不同，将能做部分调整。
根据您的样本，能够输入60种使用者标度。
RX-5000α ( alpha ) 能够显示最少30个最近的测量值。
ATAGO(爱拓) PR系列数字式折射计简单操作说明防水等级IP64，严禁用水直接冲洗！！！！一、安装1、用所配工具旋开电池室盖，装入一块9伏电池。
注意电池的极性。
二、调零（PR-32α，PR-101α，PR-201α）1、准备好蒸馏水。
2、清洁棱镜表面。
3、滴几滴蒸馏水到棱镜上，使水完全覆盖住棱镜表面。
4、按ZERO键，待显示不闪动的000时，调零完成。
5、用软布拭干棱镜。
注意：当每天第一次使用前，或环境温度变化时，或更换电池时，都要调零。
三、校正（仅PR-301α）1、准备好所提供的LG标准液。
2、清洁棱镜表面。
3、用镊子夹一张所提供的圆纸到棱镜上，滴入几滴标准液湿润圆纸（不要过量）。
4、按STANDARDIZATION键，待显示不闪动的000时，校正完成。
注意：当仪器新购置回来第一次使用前，或更换电池时，都要校正。
四、样品测量1、清洁棱镜表面。
2、滴几滴样品到棱镜上。
3、按START/OFF键。
标度符号如[1]、-2-等闪动3次后，便显示测量结果。
4、当显示000或测量值时，同时按ZERO（或STANDARDIZATION）和SCAL键，此时显示最后一次测量或调零（校正）时的温度。
五、标度转换和系数设定PR系列可进行糖度（Brix）及三种用户定义标度（2、3、4）的测量。
在使用用户标度时，应设定用户标度与糖度的转换系数。
1、解开标度锁定：按SCAL键，如显示的标度符号带中括弧[]，则表明标度被锁定，需要解开才可进行标度转换。
同时按ZERO（或STANDARDIZATION）键和START/OFF键，使标度符号不带中括弧[]。
2、标度选择：按住START/OFF约2秒，清屏。
按SCAL键至显示所需标度。
3、设定转换系数：选定一个用户标度后，按住SCAL约三秒至显示转换系数，工厂设定均为1.00。
按START/OFF或ZERO键调到所需的值。
最后按SCAL，显示END，即设定完成。
4、在标度没有锁定时，按SCAL键可进行各标度的转换。
The high-accuracy, high-quality MICROMEGA ® controllers offer unparalleled flexibility in process control. Each unit allows theuser to select the input type, from 10 thermocouple Types (J, K, T, E, R, S, B, C, N, and J DIN), Pt RTDs (100, 500, or 1000 Ω, with either 385 or 392 curve), or analog voltage or current input. The voltage/current inputs are fully scalable to engineering units, with a selectable decimal point, perfect for use with pressure, flow, or other process inputs.The MICROMEGA ® controller features a large, dual LED display, front-panel configuration, selectable temperature/ process inputs, and universal power supply that accepts 90 to 250 Vac or Vdc. Available in single- and dual-output configurations, the CN77000CN77000 R300 and R500 controllers shown with model RHS-43 hole saw for easy-to-drill round holes. See last page for ordering information.CN77333-A2 NEMA 12 style square cutout.CN77533 NEMA 4 square cutout.Shown actual size.1⁄16 DIN MICROMEGA ® Autotune PIDTemperature/Process ControllersSeries is available with relay, SSR, DC pulse, or analog voltage or current outputs. A single alarm is standard. Options include a second alarm, RS232, RS485, analog output, and remote setpoint selection.The “300” Series controllers offer many features of larger, 1⁄4 DINcontrollers in a compact, 1⁄16 DIN size. These controllers feature a 1⁄16 DIN cutout and bezel with a NEMA 12 (IP54) rating, and dual LED displays, which show different colors for the process and setpoint values. Individual indicators provide output and alarm status.The “500” features a 1⁄16 DIN cutout, 53.3 mm (2.1") square face withNEMA 4 (IP65) rating, large dual LED display, front-panel configuration, and selectable temperature/process inputs. Available in single- and dual-output configurations, theCN77500 is available with relay, SSR, DC pulse, or analog voltage or current outputs. A single alarm is standard.The R300 and R500 controllers feature a detachable display and adaptor to allow mounting in a round, 44 mm (1.75") cutout. This feature allows users to prepare the panel with a standard round hole saw as well as the conventional 1⁄16 DIN cutout.The 2-piece design snaps together, for quick, easy installation.U H igh Accuracy: ±0.5°C (0.9°F), 0.03% Rdg U H igh Quality Backed by 5-Year Warranty U U niversal Inputs—Process Voltage/Current, Thermocouple, RTD U D ual 4-Digit LED Display and Indicators for Output and Alarm Status U O ptional RS232 orRS485, OMEGA ® Protocol U R elay, SSR, DC Pulse, 0 to 10 V, and 0 to 20 mA Output TypesU R amp-to-Setpoint Feature U U niversal Power Supply, 90 to 250 Vac or Vdc U D ual Output andDual Alarm Capability U I solated Analog Output or Remote Setpoint SelectionP u t a S q u a r e C o n t r o l l e r i n a R o u n d H o l e !P a n e l P u nc h e sA v a i l a b l e , V i s i to m e g a .c o m /p a n e l p u n c h e sSpecificationsAccuracy: ±0.5°C (0.9°F) temp;0.03% rdg processResolution: 1°/0.1°; 10 µV process Temperature Stability:0.08°C/°C; 50 ppm/°C process Thermocouple Cold-End Tracking: 0.05°C/°CNMRR: 60 dBCMRR: 120 dBCommon-Mode Voltage: 1500 V peak test, 350 V per IEC spacingA/D Conversion: Dual slope Reading Rate: 3 samples/sDigital Filter: Programmable Display: Dual 4-digit, 7-segment LED, 9.2 mm (0.36"); red process variable, green setpoint; indicators for output and alarm status; 7.6 mm (0.3") for NEMA 12 (IP54) unitsWarm-Up to Rated Accuracy: 30 min InputInput Types: Thermocouple, RTD, analog voltage, analog current Thermocouple Lead Resistance:100 Ω maximumRTD Input: 2-, 3- or 4-wire; 100,500 and 1000 Ω; 0.00385 or0.00392 Pt curveVoltage Input:0 to 100 mV, 0 to 1V, 0 to 10 Vdc Current Input: 0 to 20 mA, 4 to 20 mA Configuration: Single-ended Polarity: UnipolarStep Response: 0.7 s for 99.9% Decimal Selection: None, 0.1 or 0.01 Span Adjustment:0.001 to 9999 countsOffset Adjustment: -9999 to 9999 ControlAction: Reverse (heat) or direct (cool) Modes: Time proportioning and proportional control modes; selectable preset tune, autotune, PID, proportional, proportional with integral, proportional with derivative with anti-reset windup, on/offRate: 0 to 999.9 sReset: 0 to 99 minutes, 59 seconds Cycle Time: 1 to 199 seconds;set to 0 for on/off operationGain: 0.5 to 100% of span;setpoints 1 or 2Damping: 1 to 8 in unit stepsSoak: 00.00 to 99.59 (HH.MM)Ramp to Setpoint:00.00 to 99.59 (HH.MM)Autotune: For heating onlyBreak Protection:Programmable up- or down-scale Control OutputRelay: 5 A @ 120 Vac, 3 A @ 240 Vac; configurable for on/off, PID or ramp and soak; output 1: SPDT type; output 2: SPST typeSSR: Rated 0.5 A @ 120/240 Vac, continuousDC Pulse: Non-isolated;10 Vdc @ 20 mA Analog Output: Non-isolated 0 to10 Vdc or 0 to 20 mA; 500 Ω maximumOptionsRemote Setpoint Selection:Up to 4 setpoints stored in memory;contact closure selectionAnalog Output: Isolated 0 to 10 Vdc or0 to 20 mA, programmableCommunicationsRS232 or RS485: OMEGA® protocol300 to 19.2 Kb; complete programmablesetup capability; program to transmitcurrent display, alarm status, min/max,actual measured input value and statusRS485: Addressable from 0 to 199Connection: Screw terminalsAlarmsType: SPST relay, 5 A @ 120 Vac,3 A @ 240 VacOperation: High/low, latching/non-latching and process/deviation;front-panel configurationInsulationPower to Input or Output:2500 Vac/Vdc, except alarm2 option has only 1500 Vac/Vdcbetween inputs 500 Vac/VdcGeneralPower: 90 to 250 Vac/Vdc, 50 to 400 HzOperating Ambient: 0 to 55°C(32 to 131°F), 90% RH non-condensingPower Consumption:6 VA maximum @ 120 VacPanel CutoutCN77R000 Series:44.5 mm (1.75") diameter round cutoutCN77300 and CN77500 Series:45 mm (1.772") square, 1⁄16 DINDimensions:CN77R000 Series:48 H x 48 W x 135 mm D(1.89 x 1.89 x 5.32")CN77300 Series:48 H x 48 W x 123.3 mm D(1.89 x 1.89 x 4.85")CN77500 Series:53 H x 53 W x 123.3 mm D(2.1 x 2.1 x 4.85")Weight: 227 g (0.5 lb)SPC116-S splashproof TP6.TP4.Trim plates formounting 1⁄16controller inexisting 1⁄4 DIN or1⁄8DIN cutouts.CN77500 Series NEMA 4 (IP65) faceplateRear ViewOrdering Examples: CN77533-PV, dual-output controller, 1⁄16 DIN cutout and bezel, NEMA 4/ IP65 face, with 5 A SPDT relay output 1, 5 A SPST relay output 2, and optional analog output. CN77R544-A2, controller with dual pulse-type output and second alarm relay, RHS-43, arbored hole saw.CN77353, controller with NEMA 12 face, analog output and relay output, TP4, trim plate adaptor.Programming CradleWith the MICROMEGA ®programming Cradle and our FREE software, settingup and configuring any quantity ofMICROMEGA ®controllers is fast andeasy—especially valuable for OEM applications and systems integrators.Both the CN775 and CN773front-removable MICROMEGA ®controllers with serial communicationsare quickly and easily plugged in,programmed, and removed from the cradle. The cradle includes a standard9-pin mini DIN cable for connectionto a computer’s RS232 (or RS485) serial port and is powered by 90 to250 Vac/Vdc. The free Windowsconfiguration software is menu driven and extremely user friendly (requireseither MICROMEGA ® “C2” RS232 or “C4” RS485 serial communications options).。
比重计（糖度计）使用方法【产品规格】：测量范围:1.0-1.1。
【产品说明】：测量葡萄汁的含糖量，确定发酵过程需要加多少糖。
【使用方法】：将葡萄汁装满量筒；将比重计大头朝下放入量筒中，查看液面与空气交接处，比重计显示的数值。
记录改数值，查下表就可以得到葡萄汁的含糖量。
（量筒内葡萄汁液面呈弯月形，水平读取液面弯月相切处比重计的读数值更准确）葡萄汁的比重与糖度和成酒酒精换算表比重每升葡萄汁中含糖量(克)酿成酒后含酒精量(％)1.03768 4.01.04076 4.51.04384 5.01.04795 5.61.050103 6.01.053111 6.51.0561197.01.0591277.51.0631388.11.0661468.61.0691549.01.0721629.51.07517010.01.07817810.51.08218811.01.08519611.51.08820412.01.09121212.51.09522313.11.09823113.61.10023914.01.10525014.71.10725515.01.11126615.6干红葡萄酒加糖计算公式：简易：（２４３－测出的葡萄汁糖度）×葡萄重量（千克）×0.68 （默认做13.5度）复杂：（想做的酒度*18-测出的葡萄汁糖度）*葡萄重量（千克）*葡萄出汁率葡萄酒专用酒度计使用说明：1.将酒度计漏斗端朝上，把少量葡萄酒注入漏斗，葡萄酒会沿着毛细管开始向末端移动，等到末端有几滴葡萄酒滴出时，这时把仪器颠倒过来，葡萄酒会沿着毛细管退后，在停住的那个地方直接读出的刻度就是酒的度数。
2.同样的方法测出纯净水的酒度。
3.用葡萄酒测的数值减去纯净水的数值，就接近葡萄酒的酒度。
【注意事项】1.清洗干净。
(可用高度白酒泡一下，然后用温水冲一下)2.酒的温度在20度时，测量结果最准确3. 葡萄酒里面的固形物和微粒会影响测定结果。
数字折光仪RX-5000α操作指南（供参考）1.使用注意事项关于安全事项请阅读随机操作手册2.安装电源：AC100-240V(50/60HZ)使用环境温度：5-40℃折光仪是精密仪器，不能放置在阳光直射，灰尘和震动等不良场所3.名称和元件功能3.1 RX-5000α本体a)样品台b)镜面c)盖板d)电源开关e)电源插孔f)打印机输出端子g) RS-232输出端子h)冷却吸入风扇i)排风扇3.2 RX-5000α控制面板a)模式号：指示测量和检测方法 b)日期和时间 c)量程名称 d)测量值e)测量温度（FIX） f)当前温度 g)设定温度 h)SW1,SW2,SW3和SW4开关 i)启动键（ST AR T）3.3 数字打印机DP-RX(可选件)g)在线灯 h)离线灯4.连接5.6.7.测量显示画面建立菜单设置密码RX-5000α有密码设置功能：有2种用户水平和系统水平用户水平：最多可以设置5个不同密码（ID）管理员可以改变全部操作，用户或客户只能进行测量和调零操作。
系统水平：Level3:任何操作Level2:除了送电和测量之外的其它操作Level1:用于MENU画面中信息的操作或改变Level0:用于设定密码和建立系统水平。
用户水平和系统水平的组合用“O”标记的项目，不需要密码，任何用户水平都可以操作。
5.设定测量画面测量画面有3种，用SW4选择。
5.1 MODE-1:操作在恒温模式。
温度可由用户自由设定。
在镜面上滴上样品后，按START键。
样品达到目标温度后，测量被进行。
5.2操作在恒温模式。
温度可由用户自由设定。
在镜面上滴上样品后，按START键仪器以固定间隔测量折光率和温度并且显示在目标温度下估计值。
如果用很短时间在这模式下操作，在开始测量之前样品还未到达目标温度，则这模式下的测量正确度比MODE-1差。
（如果目标温度，实际温度和样品温度相同，则测量所需时间要比MODE-1长。
5.3 MODE-3:当样品滴在镜面后按START键，仪器立即开始测量。
手持糖度计的原理及使用方法一、糖度计的工作原理光线从一种介质进入另一种介质时会产生折射现象，且入射角正弦之比恒为定值，此比值称为折光率。
果蔬汁液中可溶性固形物含量与折光率在一定条件下（同一温度、压力）成正比例，故测定果蔬汁液的折光率，可求出果蔬汁液的浓度（含糖量的多少）。
常用仪器是手持式折光仪，也称糖镜、手持式糖度计，通过测定果蔬可溶性固形物含量（含糖量），可了解果蔬的品质，大约估计果实的成熟度。
手持糖度计一般是圆柱形的。
二、手持糖度折光仪使用说明（一）、仪器结构①、折光棱镜②、盖板③、校准螺栓④、光学系统管路⑤、目镜（视度调节环）（二）、使用方法打开盖板②，用软布仔细擦净检测棱镜①。
取待测溶液数滴，臵于检测棱镜上，轻轻合上盖板，避免气泡产生，使溶液遍布棱镜表面。
将仪器进光板对准光源或明亮处，眼睛通过目镜观察视场，转动目镜调节手轮⑤，使视场的蓝白分界线清晰。
分界线的刻度值即为溶液的浓度。
（三）、校正和温度修正仪器在测量前需要校正零点。
取蒸馏水数滴，放在检测棱镜上，拧动零位调节螺钉③，使分界线调至刻度0%位臵。
然后擦净检测棱镜，进行检测。
有些型号的仪器校正时需要配臵标准液，代替蒸馏水。
另一种方法是（只适合含糖量之测定）：利用温度修正表，在环境温度下读得的数值加（或减）温度修正值，获得准确数值。
（四）、注意事项仪器系精密光学仪器，在使用和保养中应注意以下事项：1.在使用中必须细心谨慎，严格按说明使用，不得任意松动仪器各连接部分，不得跌落、碰撞，严禁发生剧烈震动。
2.使用完毕后，严禁直接放入水中清洗，应用干净软布擦拭，对于光学表面，不应碰伤，划伤。
3.仪器应放于干燥、无腐蚀气体的地方保管。
4.避免零备件丢失。
三、现在市场上的糖度计分为：非破坏式糖度计：不需将水果切开，直接将探测头接触于待测水果的表面，测定水果的甜度。
携带型糖度测定：在采摘水果之前，测定水果的甜度，以确保甜度的均一化，提升作物的生产管理目标。
（2）利用有机酸的含量与糖含量之比，可判断某些果蔬的成熟度有机酸在果蔬中的含量，因其成熟度及生长条件不同而异，一般随着成熟度提高，有机酸含量下降，而糖含量增加，糖酸比增大。
故测定酸度可判断某些果蔬的成熟度，对于确定果蔬收获及加工工艺条件很有意义2.糖度的测量方法及进展(参考文献2.3.4.6）糖度的测量方法很多,适合于检测糖溶液的方法有旋光仪法,即将一束偏振光通过糖溶液,根据偏振面转过的角度确定含糖量;另一种方法是将葡萄酒蒸馏出来的样品用阿贝折射仪,根据糖浓度与折射率的关系测定糖度。
随着设备自动化水平越来越高，在线折光仪在食品、饮料、生物、制药、化工、石化、纺织等行业广泛的应用。
例如在生产果汁时需要生产出来的果汁为一个固定的的糖度（浓度），传统的做法是人工频繁的测量果汁的浓度或者使用密度仪来测量果汁的浓度，如果浓度合格则开始杀菌、灌装。
但这两种方法都存在一定的缺陷，人工测量因为它是间隔测量很难保证所有产品浓度在合理的浓度范围内；而密度仪尽管可以连续的测量出浓度，但密度仪的工作原理决定其测量结果在有些环境下不是很准确。
密度仪的工作原理是连续的取固定体积的液体称量其重量得出密度，因为密度与浓度成一定的函数关系，从而得出液体的浓度，但是当测量物质中含有气泡、悬浮物、和结晶物质时，则密度仪得出的密度值和液体本身的密度会不一致。
折光浓度仪是根据光线在液体中的全反射和折射这种物理现象测量液体的浓度。
手持式这种是个比较普遍的测量糖方法，现在已经有大部分用户在改为使用便携式数显的糖度计来测量。
现在市场上的糖度分为：【1】非破坏式糖度计：不需将水果切开，直接将探测头接触于待测水果的表面，测定水果的甜度。
【2】携带型糖度测定：在采摘水果之前，测定水果的甜度，以确保甜度的均一化，提升作物的生产管理目标。
【3】计算机糖度测定仪：每个水果均经过甜度的测试，依据质量检测，分级、处理及包装作业，而能提升高的售价【4】高效测定食品和饮料中的糖分含量4.1根据折光率仪测定白利糖度早先用阿贝折光率仪测量折射率。
‘一．概述：EL—9000A型智能流量显示仪，能与输出脉冲信号的流量计配套使用，可以精确地测定和显示累计流量、瞬时流量、百分比流量，具有等量同步输出流量脉冲。
EL—9000B型流量显示仪还同时输出对应于瞬时流量大小的4~20mA电流，作为远传显示，控制调节之用。
EL—9000C是在EL—9000B的基础上增加LED光柱直观显示瞬时流量。
本机采用最新单片机技术，具有自动掉电保护功能、操作简单、抗干扰性能强，是新一代智能流量显示仪表。
二．主要技术参数：1．输入：① QFⅠ信号：二线制电压脉冲；QFⅡ信号：三线制电压脉冲② Ag19信号：二线制电流脉冲；③ GF1信号：三线制电压脉冲；④ VA信号：二线制电流脉冲；2．输出：①电压脉冲信号：高电平>5V，低电平<0.5V（与输入流量脉冲信号同步，等量）②模拟量：4～20mA DC （EL—9000B、C型有此功能）或模拟量：1~5V DC3．基本误差：总量基本误差Δqm=±0.1% q –总量的理论计算值=±0.2% FS FS –输出电流量程。
模拟量输出基本误差：ΔIm4．显示：LED显示器八位，状态灯四只累计量00000.000~99999999升或立方瞬时量00000.000~99999999升/分或立方/小时5．掉电数据保护：累计量，单班量，仪表设定参数6．供电电源：AC220V（±10%）50Hz7．正常工作条件：环境温度：0℃~40℃相对湿度：≤85%周围空气中不含有腐蚀性气体三．操作：1．显示仪前面板（图1）的操作：转换键：点击操作，用于不同显示量之间的转换。
清零键：点击此键可将单班量清零，操作此键需慎重。
复位键：当显示仪由于受到非常严重的干扰工作不正正常时，点击此键可 恢复正常,操作此键需慎重。
显示仪上电后即显示—EL9000--，片刻后进入单班累计量显示，此时L 灯 （升）或M 3（立方）灯亮；点击转换键，进入累计量显示，此时Σ,L 灯亮，显 示为总累计量升，或Σ，M 3灯亮，显示为总累计量立方，累计量显示时，当小数 点位前面计量值全满时，如9999-9.××，小数自动右移一位，扩大累计范围； 再点击转换键,进入瞬时量显示，面板显示为L □□□□□□□ ，表示显示的是 瞬时量；再点击转换键，进入百分比流量显示，%灯亮，显示为□□□□.□□ 显示现在流量是已置入的最大流量的百分之几，当超过设定的百分比流量上限时，%灯闪烁；再点击转换键又回到单班累计量显示。
●PAL-α手持糖度计使用SOP1. 目的Objective / Purpose规定PAL-α手持糖度计标准操作和维护程序，确保仪器正常运行。
2. 适用范围Scope本公司质量检验室PAL-α手持糖度计。
3. 责任部门（人）及权限Responsible department (person) and authority操作人员依照规程进行使用操作，使用后及时登记。
4. 定义、符号及缩略语Definition, Signal & Abbreviations无。
5. 物料和设备material and equipment擦镜纸、纯化水。
6. 规程Procedure6.1. 操作6.1.1开机：按下并按住START键约2秒。
6.1.2零点设置：每次使用前必须进行零点设置6.1.2.1清洁棱镜表面6.1.2.2准备纯化水，放置至室温。
6.1.2.3将约0.3ml的水到棱镜表面6.1.2.4按START键。
如果“ --- ”出现，那么将显示测得的糖度值（％），以及在测定时样品的温度。
6.1.2.5如果显示屏上显示“0.0％”，零点设置并不需要执行。
用擦镜纸将棱镜表面的水擦净，PAL-α手持糖度计可以进行下一步样品测试。
6.1.2.6如果指定的值是不是“0.0”按下ZERO键6.1.2.7闪烁2次后，“000”将显示在LCD屏幕上，如果显示“AAA”，添加更多的水到棱镜表面，并再次按下ZERO键。
6.1.2.8当显示“000”，零点设置已经成功地完成了。
用擦镜纸将棱镜表面的水擦净，擦干棱镜表面，用纸巾擦拭,PAL-α手持糖度计可以进行下一步样品测试。
6.1.3测量方法6.1.3.1清洁棱镜表面。
6.1.3.2将样品放置室温，取样品约0.3ml放置到棱镜表面上6.1.3.3按START键，如果“ --- ”出现，那么将显示测得的糖度值（％），以及在测定时样品的温度，测量值将持续显示约2分钟关闭显示屏。
血糖含量检测试剂盒说明书UPLC-MS-4642100T/96S试剂名称规格保存条件试剂一液体10mL×1瓶2-8℃保存试剂二液体10mL×1瓶2-8℃保存试剂三液体10mL×1瓶2-8℃保存溶液的配制：1、试剂一：2μmol/mL 葡萄糖溶液；2、混合试剂的配制：使用前将试剂二和试剂三1:1等体积混合，用多少配多少。
微量法哺乳动物血液中的葡萄糖称为血糖，是其体内糖的主要运输形式。
血糖浓度受神经系统和激素的调节而保持相对稳定，调节失衡时出现高血糖和低血糖。
糖尿病、颅内压增加和脱水症等均可引起高血糖；饭后，精神紧张也可出现生理性高血糖。
相反，胰岛β细胞增生或肿癌等，垂体、肾上腺皮质和甲状腺功能减退，以及严重肝病患者均可出现低血糖症状。
此外，饥饿和剧烈运动可引起暂时的低血糖。
葡萄糖氧化酶能催化葡萄糖氧化成葡萄糖酸，并产生过氧化氢；过氧化物酶催化过氧化氢氧化4-氨基安替比林偶联酚，生成有色化合物，在505nm 有特征吸收峰。
Aldehydo-D-GlucoseGlucose OxidaseD-Gluconic Acid +H 2O 2H 2O 2+4-AminoantipyrinePeroxidaseColored Compound (Quinone Compounds)(505nm )最低检出限：0.0188μmol/mL 线性范围：0.125-8μmol/mL注意：实验之前建议选择2-3个预期差异大的样本做预实验。
如果样本吸光值不在测量范围内建议稀释或者增加样本量进行检测。
可见分光光度计/酶标仪、水浴锅/恒温培养箱、可调式移液器、微量玻璃比色皿/96孔板和蒸馏水。
一、样本处理（可适当调整待测样本量，具体比例可以参考文献）直接测定。
二、测定步骤1、分光光度计/酶标仪预热30min 以上，调节波长至505nm ，分光光度计用蒸馏水调零。
2、样本测定（在1.5mL EP 管或96孔板中依次加入下列试剂）：试剂（μL）空白管标准管测定管样本--20试剂一-20-蒸馏水20--混合试剂180180180涡旋混匀，置于37℃（哺乳动物）水浴锅/恒温培养箱或25℃（其它物种）水浴锅中准确保温15min后，于505nm波长处读取吸光度A，分别记为A空白、A标准和A测定，标准管和空白管只需测1-2次。
