(-)-Epicatechin_490-46-0_DataSheet_MedChemExpress

MED-4920Liquid Silicone RubberSILICONE TECHNOLOGYCreative Partners in a Material WorldNuSil Technology 1050 Cindy Lane • Carpinteria, CA 93013 805/684-8780 • 805/566-9905 Fax An ISO 9001 Certified CompanyProduct ProfileDescription• • • Two-part, translucent silicone system used with injection molding equipment Rapid cure with no post-cure required 1:1 Mix Ratio (Part A:B)Applications• For the injection molding of parts requiring a material with a low durometer, including: gaskets, seals, molded balloons, and catheter tipsNuSil Technology’s MED-4920 is a restricted product. It shall not be considered for use in human implantation for a period of greater than 29 days.ResultTranslucent 35 g/min 40 g/min >24 hours 1.13 20 1,000 psi 900% 125 ppi 65 psiTypical PropertiesUncured: Appearance Extrusion Rate*, Part A Extrusion Rate*, Part B Work Time Cured: 5 minutes @ 150°C (302°F) Specific Gravity Durometer, Type A Tensile Strength Elongation Tear Strength Stress @ 200% StrainMetric Conv.6.9 MPa 22.0 kN/m 0.4 MPaASTMD2090 C603 C603 D792 D2240 D412, D882 D412, D882 D624 D412, D882NT-TM002 033 033 008 003 006 007 007 009 007* Performed using a SEMCO 440 nozzle with a 1/8” orifice and 90+/-5 psi air pressureInstructions for UseMixingUse with mix and dispense equipment in a 1:1 mix ratio. If mixing by hand, take care to minimize air entrapment.Packaging50 ml SxS Kit 400 ml SxS Kit 2 Pint Kit (910 g) 2 Gallon Kit (7.28 kg) 10 Gallon Kit (36.4 kg)Vacuum DeaerationRemove air entrapped during mixing by common vacuum deaeration procedure, observing all applicable safety precautions. Slowly apply vacuum, up to 28 inches Hg, to a container rated for use and of volume at least four times that of material being deaerated. Hold vacuum until presence of air is no longer evident.Warranty12 MonthsSubstrate ConsiderationCures in contact with most materials common to biomedical assemblies. Exceptions include butyl, latex, chlorinated rubbers, some RTV silicones and unreacted residues of some curing agents.Adjustable Cure ScheduleProduct cures at a wide range of cure times and temperatures to accommodate different production needs. Contact NuSil Technology for details.MED-4920 05 April 2007FDA Master Access FileA Master Access File for MED-4920 has been filed with the U.S. Food and Drug Administration. Customers interested in authorization to reference the Master Access File must contact NuSil Technology.Warnings About Product SafetyNuSil Technology believes that the information and the data contained herein are accurate and reliable. However, the user is responsible to determine the material’s suitability and safety of use. NuSil Technology cannot know each application’s specific requirements and hereby notifies the user that it has not tested or determined this material’s suitability or safety for use in any application. The user is responsible to adequately test and determine the safety and suitability for their application and NuSil Technology makes no warranty concerning fitness for any use or purpose. NuSil Technology has completed no testing to establish safety of use in any medical application. NuSil Technology has tested this material only to determine if the product meets the applicable specifications. (Please contact NuSil Technology for assistance and recommendations when establishing specifications.) When considering the use of NuSil Technology products in a particular application, review the latest Material Safety Data Sheet and contact NuSil Technology with any questions about product safety information. Do not use any chemical in a food, drug, cosmetic, or medical application or process until having determined the safety and legality of the use. The user is responsible to meet the requirements of the U.S. Food and Drug Administration (FDA) and any other regulatory agencies. Before handling any other materials mentioned in the text, obtain available product safety information and take the necessary steps to ensure safety of use.SpecificationsDo not use the typical properties shown in this technical profile as a basis for preparing specifications. Please contact NuSil Technology for assistance and recommendations in establishing particular specifications.Patent WarningNuSil Technology disclaims any expressed or implied warranty against the infringement of any patent. NuSil Technology does not warrant the use or sale of the products described herein will not infringe the claims of any United States’ or other country’s patents covering the product itself, its use in combination with other products or its use in the operation of any process.Warranty InformationNuSil Technology’s warranty period is 12 months from the date of shipment when stored below 40°C in original unopened containers. Unless NuSil Technology provides a specific written warranty of fitness for a particular use, NuSil Technology’s sole warranty is that the product will meet NuSil Technology’s then current specification. NuSil Technology specifically disclaims any other expressed or implied warranty, including warranties of merchantability and fitness for use. The exclusive remedy and NuSil Technology’s sole liability for breach of warranty is limited to refund of purchase price or replacement of any product shown to be other than as warranted. NuSil Technology expressly disclaims any liability for incidental or consequential damages.MED-4920 05 April 2007。

天然产物化学单体Name 中⽂Cas No PuritAconitine 乌头碱302-27-2 98%Ailanthone 臭椿酮981-15-7 98%Alantolactone ⼟⽊⾹内酯546-43-0 98%Albiflorin 芍药内酯苷39011-90-0 0.98Alisol B,23-acetate 23-⼄酰泽泻醇B 19865-76-0 0.98 Allomatrine 槐定碱641-39-4 0.98Amentoflavone 穗花杉双黄酮1617-53-4 0.98 Andrographolide 穿⼼莲内酯5508-58-7 0.98Anwuligan 安五脂素107534-93-0 0.98Atractylenolide I ⽩术内酯I 73069-13-3 0.98Atractylenolide Ⅲ⽩术内酯Ⅲ73030-71-4 98%(-)-Asarinin 细⾟脂素133-04-0 98%Atractylodin 苍术素55290-63-6 98%Arenobufagin 沙蟾蜍精464-74-4 98%Adenosine 腺苷58-61-7 98%Arctigenin ⽜蒡⼦苷元7770-78-7 98%Arctiin ⽜蒡⼦苷20362-31-6 98%Astilbin 落新妇苷29838-67-3 98%Akebia saponin D ⽊通皂苷D 39524-08-8 98%Atractylodin 西贝母碱61825-98-7 98%Astragalin 紫云英苷480-10-4 98%Baohuoside I 宝藿苷I 113558-15-9 98%Bengenin 岩⽩菜素477-90-7 98%Benzoylpaeoniflorin 苯甲酰芍药苷38642-49-8 98%Beta-boswellic acid beta-乳⾹酸631-69-6 98%Beta-boswellic acid,11-keto 11-酮基乳⾹酸17019-92-0 98% Bilobalide ⽩果内酯33570-04-6 98% Bisdemethoxycurcumin 双去甲氧基姜黄素33171-05-0 98% Britannilactone 旋覆花内酯33627-41-7 98%1-O-acetyl Britannilactone 1-氧-⼄酰旋覆花内酯681457-46-5 98% Bufalin 蟾毒灵465-21-4 98%Bufotalin 蟾蜍他灵471-95-4 98%Betulin ⽩桦酯醇473-98-3 98%Betulinic acid ⽩桦酯酸472-15-1 98%Carnosic acid ⿏尾草酸6537-80-8 98%Carnosol ⿏尾草酚5957-80-2 98%Catechin gallate ⼉茶素没⾷⼦酸酯130405-40-2 98%Celastrol 雷公藤红素34157-83-0 98%Catechin（C）⼉茶素154-23-4 98%Chlorogenic acid 绿原酸327-97-9 98%Cichoric acid 菊苣酸70831-56-0 98%Cinobufagin 华蟾酥毒基470-37-1 98%cimifugin 升⿇素37921-38-3 98%Cordycepin ⾍草素73-03-0 98%Corosolic acid 科罗索酸4547-24-4 98%(+)-corynoline 紫堇灵18797-79-0 98%Corynoxine 去氢钩藤碱6877-32-3 98%Costunolide ⽊⾹烃内酯553-21-9 98% Cryptochlorogenin Acid 隐绿原酸905-99-7 98% Cucurbitacin B 葫芦素B 6199-67-3 95% Cucurbitacin E 葫芦素E 18444-66-1 95% Cinobufotalin 华蟾毒它灵1108-68-5 98%Curcumol 莪术醇4871-97-0 98%Cynarin 洋蓟素1182-34-9 98%Chelerythrine chloride ⽩屈菜红碱34316-15-9 98% Corilagin 柯⾥拉京23094-69-1 98%Cardamonin ⼩⾖蔻明19309-14-9 98%Cistanoside A ⾁苁蓉苷A 93236-42-1 98% Curcumin 姜黄素458-37-7 98%Echinacoside 松果菊苷82854-37-3 98% Epicatechin 表⼉茶素490-46-0 98%Epicatechin gallate ECG 1257-08-5 98% Epigallocatechin 表没⾷⼦⼉茶素970-74-1 98% Epimedin A 朝藿定A 110623-72-8 98%Epimedin B 朝藿定B 110623-73-9 98%Epimedin C 朝藿定C 110642-44-9 98% Ecdysterone 蜕⽪激素5289-74-7 98%4F of perissemipinnatal 半边旗4F 98%5F of perissemipinnatal 半边旗5F 98%Eupatilin 异泽兰黄素22368-21-4 98% Forsythiaside 连翘酯苷79916-77-1 98% Fangchinoline 防⼰诺林碱/汉防已⼄素436-77-1Daidzein ⼤⾖苷元486-66-8 98%Daidzin ⼤⾖苷552-66-9 98% Dehydrocostuslactone 去氢⽊⾹烃内酯477-43-0 98% Demethoxyyangonin 去甲氧基醉椒素15345-89-8 98% Desacetylcinobufagin 去⼄酰华蟾毒精95% Desacetylcinobufotalin 去⼄酰华蟾毒它灵95% Dictamnine ⽩鲜碱484-29-1 98% Demethoxycurcumin 去甲氧基姜黄素33171-16-3 98% Dendrobine ⽯斛碱2115-91-5 98%Ginkgolic Acid C13:0 银杏酸C13:0 20261-38-5 98% Ginkgolic Acid C15:1 银杏酸C15:1 22910-60-7 98% Ginkgolic AcidC17:1 银杏酸C17:1 111047-30-4 98% Glycitein 黄⾖黄素40957-83-3 98%Glycitin 黄⾖黄苷40246-10-4 98%Ginsenoside Rb1 ⼈参皂苷Rb1 41753-43-9 98%Ginsenoside Rb2 ⼈参皂苷Rb2 11021-13-9 98% Ginsenoside Rb3 ⼈参皂苷Rb3 68406-26-8 98% Ginsenoside Rg1 ⼈参皂苷Rg1 22427-39-0 98% Ginsenoside Rg2 ⼈参皂苷Rg2 52286-74-5 98% Ginsenoside Rg3 ⼈参皂苷Rg3 14197-60-5 98% Ginsenoside Rh1 ⼈参皂苷Rh1 63223-86-9 98% Ginsenoside Rh2 ⼈参皂苷Rh2 78214-33-2 98% Ginsenoside Rh3 ⼈参皂苷Rh3 105558-26-7 98% Ginsenoside Rc ⼈参皂苷Rc 11021-14-0 98%Ginsenoside Re ⼈参皂苷Re 52286-59-6 98%Ginsenoside Rd ⼈参皂苷Rd 52705-93-8 98%Ginsenoside CK ⼈参皂苷CK 39262-14-1 95% Ginsenoside- Rf ⼈参皂苷-Rf 52286-58-5 98% Gamabufotalin 和蟾蜍他灵465-11-2 98%Genistein 染料⽊甙529-59-9 98%Ginsenoside F1 ⼈参皂苷F1 53963-43-2 98%Genkwanin 芫花素437-64-9 98%genipin 京尼平6902-77-8 98%Gelsemine 钩吻碱509-15-9 98%Genipin-1-b-D-gentiobioside 京尼平－龙胆双糖苷98% Geniposide 京尼平苷24512-63-8 98%Geniposidic acid 京尼平苷酸"27741-01-1" 98%Gypenoside XVII 七叶胆苷XVII 80321-69-3 98% Hinokiflavone 扁柏双黄酮19202-36-9 98% Hmoharringtonine ⾼三尖杉酯碱26833-87-4 98% Homoorientin 异荭草苷4261-42-1 98%Honokiol 和厚朴酚35354-74-6 98%Hydroxy safflor yellow A 羟基红花黄⾊素A 78281-02-4 98% Hypaconitine 次乌头碱6900-87-4 98%Hypericin ⾦丝桃素548-04-9 98%Hyperoside ⾦丝桃苷482-36-0 98%hydroxygenkwanin 羟基芫花素20243-59-8 98%Icariin 淫⽺藿苷489-32-7 98%Imperatorin 欧前胡素482-44-0 98%Ikarisoside F ⼤花淫⽺藿苷F 113558-14-8 90% Isoalantolactone 异⼟⽊⾹内酯470-17-7 98%(+)-isocorynoline 右旋异紫堇定475-67-2 98% Iisoimperatorin 异欧前胡素482-45-1 98% Isorhynchophylline 异钩藤碱6859-01-4 98% Isochlorogenic acid A 异绿原酸A 2450-53-5 98% Isochlorogenic acid B 异绿原酸B 14534-61-3 98% Isorhamnetin 异⿏李素480-19-3 98%Koumine 钩吻素⼦1358-76-5 98%L-4-Hydroxyisoleucine L-4羟基异亮氨酸6001-78-8 98% Liquiritigenin ⽢草素578-86-9 98%Luteoloside ⽊犀草苷5373-11-5 98%Levistilide A 欧当归内酯A 88182-33-6 98% Madecasoside 羟基积雪草苷34540-22-2 98%Matrine 苦参碱519-02-8 98%(±)-Maackiain 马卡因19908-48-6 98%Mesaconitine 新乌头碱2752-64-9 98%Methysticin ⿇醉椒苫素20697-20-5 98%Mollugin ⼤叶茜草素55481-88-4 98%Morroniside 莫诺苷25406-64-8 98%Nochlorogenic acid 新绿原酸906-33-2 98% Neogambogic acid 新藤黄酸93772-31-7 98% Nuciferine 荷叶碱475-83-2 98% (R)Notoginsenoside R2 三七皂苷R2(R型）98% Notoginsenoside Fe 三七皂苷Fe 98% Notoginsenoside Fe 三七皂苷Ft1 98% Neomangiferin 新芒果苷64809-67-2 98%Neferine 甲基莲⼼碱2292-16-2 98%Oridonin 冬凌草甲素28957-04-2 98%Osthol 蛇床⼦素484-12-8 98%Oxymatrine 氧化苦参碱16837-52-8 98% Oxypaeoniflorin 羟基芍药苷39011-91-1 98%Ononin 芒柄花苷486-62-4 98%Orcinol glucoside 苔⿊酚葡萄糖苷21082-33-7 98% Pillyrin 连翘苷487-41-2 98%Plydatin 虎杖苷27208-80-6 98%Paeruptorin B ⽩花前胡⼄素81740-07-0 98% Pseudolaric acid B ⼟荆⽪⼄酸82508-31-4 98%Prim-o-glucosylcimifugin 升⿇素苷80681-45-4 98% Peoniflorin 芍药苷23180-57-6 98%Psoralen 补⾻脂素66-97-7 98%Phloretin 根⽪素60-82-2 98%Phloridzin 根⽪甙60-81-1 98%Pseudoginsenoside F11 伪⼈参皂苷F11 69884-00-0 98% Protopanoxadiol 原⼈参⼆醇7755-01-3 98% Protopanaxatriol 原⼈参三醇1453-93-6 98% Panaxadiol ⼈参⼆醇19666-76-3 98%Panaxatriol ⼈参三醇32791-84-7 98%Polyphyllin I 重楼皂苷I 50773-41-6 98%Polyphyllin II 重楼皂苷II 76296-72-5 98% Paeruptorin A ⽩花前胡甲素73069-25-7 98% Pedunculoside 具栖冬青苷42719-32-4 98% Protodioscin 原薯蓣皂苷55056-80-9 98%Peimisine 贝母⾟19773-24-1 98%Peimine 贝母甲素23496-41-5 98%Peiminine 贝母⼄素18059-10-4 98%Phellodendrine 黄柏碱6873-13-8 98%Quercitrin 槲⽪苷522-12-3 98%Resibufogenin 酯蟾毒配基465-39-4 98%Rhmannioside D 地黄苷D 81720-08-3 98%Resveratrol ⽩藜芦醇501-36-0 98%Rhynchophylline 钩藤碱76-66-4 98%Rhmannioside A 地黄苷A 81720-05-0 98%Rosavin 洛塞维84954-92-7 98%Ruscogenin 鲁斯可皂苷元472-11-7 98%Salvianolic acid B 丹酚酸B 115939-25-8 98%Sanggenon C 桑根酮C 80651-76-9 98%Secoisolariciresinol Diglucoside 亚⿇⽊酚素(SDG) 148244-82-0 98% Sennoside A 番泻苷A 81-27-6 98%Sennoside B 番泻苷B 128-57-4 98%Sesamin 芝⿇素607-80-7 98%Shikonin 紫草素517-89-5 98%Schisandrin A 五味⼦素甲61281-38-7 98%Schisandrin B 五味⼦素⼄61281-37-6 98% Schisantherin A 五味⼦酯甲58546-56-8 98% schizandrol B 五味⼦醇⼄58546-54-6 98% Shionone 紫苑酮10376-48-4 98% Sagittatoside A 箭藿苷A 118525-35-2 98% Sagittatosdie B 箭藿苷B 118525-36-3 98% Sophocarpidine 氧化槐果碱26904-63-3 98% Sophocarpine 槐果碱145572-44-7 98% Syringin 紫丁⾹苷118-34-3 98% Sanguinarine ⾎根碱2447-54-3 98% Solasodine 澳洲茄胺126-17-0 98% Senegenin 远志皂苷元2469-34-3 98% Tabersonine 它波宁4429-63-4 98% Tetrahydropalmatine 延胡索⼄素2934-97-6 98% Tiliroside 银椴苷20316-62-5 98% Turkesterone ⼟克甾酮41451-87-0 98% Tectoridin 射⼲苷611-40-5 98%Taraxerol 蒲公英萜醇98%Vitexin 牡荆素3681-93-4 98%vitexicarpin 蔓荆⼦黄素479-91-4 98% wedelolactone 蟛蜞菊内酯524-12-9 98% yangonin 甲氧醉椒素500-62-0 98% Telocinobufagin 远华蟾蜍精472-26-4 98%第⼀版前⾔缩写表第⼀章概论第⼆章天然产物的提取分离第三章结构研究中常⽤的波谱技术第四章⽣物碱第五章单萜第六章倍半萜类第七章昆⾍激素与信息素第⼋章⼆萜类化合物第九章银杏萜内酯第⼗章皂苷第⼗⼀章氨基酸、肽、蛋⽩质第⼗⼆章碳⽔化合物第⼗三章黄酮类化合物第⼗四章葸醌类化合物第⼗五章⾹⾖素类化合第⼗六章⽊脂体类化合物第⼗七章碳环芳⾹族酸酚性化合物第⼗⼋章强⼼苷类化合物第⼗九章其他⽣物活性天然化合物第⼆⼗章海洋天然产物第⼆⼗⼀章天然产物的结构修饰第⼆⼗⼆章天然产物的化学合成第⼆⼗三章天然化合物的⽣物合成。

化学纤维儿茶素含量的测定高效液相色谱法警告：使用本文件的人员应有正规实验室工作的实践经验。

本文件并未指出所有可能的安全问题。

使用者有责任采取适当的安全和健康措施，并保证符合国家有关法规规定的条件。

1 范围本文件描述了采用高效液相色谱法测定儿茶素改性纤维中儿茶素含量的方法。

本文件适用于含有儿茶素活性成分的化学纤维的儿茶素含量的检测。

2 规范性引用文件下列文件中的内容通过文中的规范性引用而构成本文件必不可少的条款。

其中，注日期的引用文件，仅该日期对应的版本适用于本文件；不注日期的引用文件，其最新版本（包括所有的修改单）适用于本文件。

GB/T 603 化学试剂试验方法中所用制剂及制品的制备GB/T 6682 分析实验室用水规格和试验方法3试验原理利用甲醇萃取纤维样品中的儿茶素类活性成分，利用高效液相色谱法进行儿茶素类活性成分的定性定量分析。

4试剂和材料除非另有说明，在分析中仅使用确认为分析纯的试剂和符合GB/T 6682规定的一级水。

4.1 儿茶素类标准物质：儿茶素（CAS登录号154-23-4）、表儿茶素（CAS登录号490-46-0）、表没食子儿茶素（CAS登录号970-74-1）、表儿茶素没食子酸酯（CAS登录号1257-08-5）、表没食子儿茶素没食子酸酯(CAS登录号989-51-5)，色谱纯，纯度≥98%。

4.2 试剂：甲醇，甲酸，甲酸铵，乙腈，色谱纯。

5仪器和设备5.1 高效液相色谱仪（HPLC）：配置SPD-M40二极管阵列检测器。

5.2 天平：实际分度值0.1 mg。

5.3 螺纹口样品瓶：2 mL，可用于高效液相色谱自动进样，配套聚四氟乙烯内垫。

5.4 超声波清洗仪：功率400 W，频率40 kHz，配500 mL提取瓶。

5.5 布氏漏斗、抽滤瓶。

5.6 定性滤纸：不含脂，中速。

126 5.7 旋转蒸发仪：配旋蒸瓶。

5.80.45 μm 微孔滤膜。

5.9量筒。

5.10 刻度移液管或移液枪：1 mL 。

©2018 QIAGEN Beverly, Inc. 100 Cummings Center Suite 407J Beverly, MA 01915 Quantabio brand products are manufactured by QIAGEN Beverly, Inc.Intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.95139 / IFU-095.1 REV 02PerfeC T a ® qPCR ToughMix ® UNG ROX ™DescriptionPerfeC T a qPCR ToughMix UNG ROX is a 2X concentrated ready-to-use reaction cocktail for PCR amplification of DNA templates that overcomes many known inhibitors of PCR often present in crude samples extracted from environmental specimens, plant tissues, or animal tissues. It is a versatile and robust real-time qPCR reagent that provides maximum sensitivity and PCR efficiency with a variety of fluorogenic probe chemistries, including TaqMan ® hydrolysis probes. PerfeC T a qPCR ToughMix UNG ROX contains all required reaction components, except primers, probe(s), and DNA template. Inclusion of uracil DNA glycosylase (UNG), and substitution of dTTP with dUTP, prevents amplification of carry-over contamination from previous dU-containing PCRs.A key component of PerfeC T a qPCR ToughMix UNG ROX is an ultra pure, highly processive thermostable DNA polymerase that is combined with high avidity monoclonal antibodies. This proprietary polymerase mix is highly resistant to PCR inhibitors and provides an extremely stringent automatic hot-start allowing reaction assembly, and temporary storage, at room temperature prior to PCR amplification. PerfeC T a qPCR ToughMix UNG ROX delivers exceptional performance with either fast or conventional PCR cycling protocols.Instrument CompatibilityDifferent real-time PCR systems employ different strategies for the normalization of fluorescent signals and correction of well-to-well optical variations. It is important to match the appropriate reference dye to each specific optical detection system. PerfeCta qPCR ToughMix, ROX contains an optimal concentration of a stabilized carboxy-X-rhodamine compound (ROX ™) for instruments that use an excitation wavelength of ~490 nm and 605 to 610 nm emission channel for the reference signal. Please consult our Product Finder selection tool at to find the correct product for your real-time PCR system. ComponentsPerfeC T a qPCR ToughMix UNG ROX (2X): 2X concentrated reaction buffer containing optimized concentrations of MgCl 2, dNTPs (dATP, dCTP, dGTP,dUTP), hot-start DNA polymerase, uracil DNA glycosylase (UNG), and stabilizers.Storage and StabilityStore components in a constant temperature freezer at -25°C to -15°C protected from light upon receipt. Repeated freezing and thawing does not impair product performance. After thawing, mix thoroughly before using.For lot specific expiry date, refer to package label, Certificate of Analysis or Product Specification Form.Guidelines for qPCR:▪ The design of highly specific primers and probes is a critical parameter for successful real-time PCR. The use of computer aided primer design programs is encouraged in order to minimize the potential for internal secondary stru cture and complementation at 3’-ends within each primer, the primer pair, and primer/probe combinations. For best results, amplicon size should be limited to 65 - 200 bp. Optimal results may require titration of primer concentration between 100 and 900 nM. A final concentration of 300 - 400 nM each primer and 100 to 250 nM probe is effective for most applications. Increasing the concentration of the primer that initiates synthesis of the target strand that is complementary to the probe can improve fluorescent signal for some primer/probe systems.▪ Preparation of a reaction cocktail is recommended to reduce pipetting errors and maximize assay precision. Assemble the reaction cocktail with all required components except sample template (genomic DNA or cDNA) and dispense equal aliquots into each reaction tube. Add the DNA template to each reaction as the final step. Addition of samples as 2 to 5- L volumes will improve assay precision.▪ Suggested input quantities of template are: cDNA corresponding to 1 pg to 100 ng of total RNA; 10 pg to 1 µg genomic DNA▪ After sealing each reaction, vortex gently to mix contents. Centrifuge briefly to collect components at the bottom of the reaction tube. Reaction AssemblyComponentVolume for 20-μL rxn.Final ConcentrationPerfeC T a qPCR ToughMix UNG ROX (2X) 10 µL 1xForward primer variable 100 – 900 nM Reverse primer variable 100 – 900 nM Probevariable 100 – 250 nMNuclease-free water variable Template2 – 5 µL variable Final Volume (μL)20 µLNote : For smaller or larger reaction volumes scale all components proportionally.Cat No. 95139-250 Size: 250 x 20-uL reactions (2 x 1.25 mL) Store at -25ºC to - 15°C protected from light95139-012 1250 x 20-µL reactions (10 x 1.25 mL)95139-05K5000 x 20-µL reactions (1 x 50 mL)©2018 QIAGEN Beverly, Inc. 100 Cummings Center Suite 407J Beverly, MA 01915 Quantabio brand products are manufactured by QIAGEN Beverly, Inc.Intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.95139 / IFU-095.1 REV 02PCR Cycling ProtocolFast 2-Step Cycling Fast 3-Step Cycling Standard Cycling UNG carry-over incubation – optional:Initial denaturation: PCR cycling (30-45 cycles):The appropriate step for fluorescent data collection varies for different probe assay formats. Data collection for 5’-nuclease probe assays (TaqMan probe) should be carried out at the end of the extension step. Use the annealing step for data collection with hybridization probe assays (HybProbe ® FRET hybridization probes, Molecular Beacons, Solaris ® qPCR Assays, Scorpions ® primers, etc.).End-point analysis should be carried out at a suitable temperature for your detection probe chemistry.‡ UNG incubation is optional. Alternate protocols are acceptable. We find that a 5 minute incubation at 45ºC is significantly more effective at eliminating carry-over contamination than the more typical procedure of 50ºC for 2 min.* Full activation of the DNA polymerase occurs within 10 seconds at 95ºC; however, optimal initial denaturation time is template dependent and will affect qPCR efficiency and sensitivity. Amplification of genomic DNA or supercoiled plasmid DNA targets may require 5 to 10 min at 95ºC to fully denature and fragment the template. Short double-stranded DNA template (PCR product) or single-stranded DNA template, such as cDNA, may require as little as 1s at 95ºC. Use 30s at 95ºC as a general starting point.† Extension time is dependent upon amplicon length and the minimal data collection time requirement for your qPCR instrument. Use 30s at 60ºC as a general starting point. Some assay designs and/or detection chemistries may require a 3-step cycling protocol for optimal performance. Optimal annealing temperature and time may need to be empirically determined for any given primer set and real-time instrument.Quality ControlKit components are free of contaminating DNase and RNase. PerfeC T a qPCR ToughMix UNG ROX is functionally tested in qPCR. Kinetic analysis must demonstrate linear resolution over six orders of dynamic range (R 2 > 0.990) with a 2-fold discrimination of starting template and a PCR efficiency > 95%.Limited Label LicensesUse of this product signifies the agreement of any purchaser or user of the product to the following terms:1. The product may be used solely in accordance with the protocols provided with the product and this manual and for use with components contained in the kit only. QIAGEN Beverly, Inc. grants no license under any of its intellectual property to use or incorporated the enclosed components of this kit with any components not included within this kit except as described in the protocols provided with the product, this manual, and additional protocols available at Some of these additional protocols have been provided by Quantabio Product users for Quantabio users. These protocols have not been thoroughly tested or optimized by QIAGEN Beverly, Inc.. QIAGEN Beverly, Inc. neither guarantees them nor warrants that they do not infringe the rights of third-parties.2. Other than expressly stated licenses, QIAGEN Beverly, Inc. makes no warranty that this kit and/or its use(s) do not infringe the rights of third-parties.3. This kit and its components are licensed for one-time use and may not be reused, refurbished, or resold.4. QIAGEN Beverly, Inc. Specifically disclaims any other licenses, expressed or implied other than those expressly stated.5. The purchaser and user of the kit agree not to take or permit anyone else to take any steps that could lead to or facilitate any acts prohibited above. QIAGEN Beverly, Inc. may enforce the prohibitions of this Limited License Agreement in any Court, and shall recover all its investigative and Court costs, including attorney fees, in any action to enforce this Limited License Agreement or any of its intellectual property rights relating to the kit and/or its components.This product is licensed under U.S. Patent No. 7,972,828 and corresponding US and foreign patents and patent applications for any use for research and development purposes; the license expressly excludes any use for diagnostic testing or clinical therapeutics in humans or animals.The use of this product is covered by at least one claim of U.S. Patent No. 7,687,247 owned by Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The buyer cannot sell or otherwise transfer (a) this product, (b) its components, or (c) materials made by the employment of this product or its components to a third party or otherwise use this product or its components or materials made by the employment of this product or its components for Commercial Purposes. Commercial Purposes means any activity for which a party receives or is due to receive consideration and may include, but is not limited to: (1) use of the product or its components in manufacturing; (2) use of the product or its components to provide a service, information, or data; (3) use of the product or its components for therapeutic, diagnostic or prophylactic purposes; or (4) resale of the product or its components, whether or not such product or its components are resold for use in research. The buyer cannot use this product or its components or materials made using this product or its components for therapeutic, diagnostic or prophylactic purposes. Further information on purchasing licenses under the above patents may be obtained by contacting the Licensing Department, Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008. Email: *************************.PerfeC T a, and ToughMix are registered trademarks of QIAGEN Beverly, Inc.. TaqMan is a registered trademark of Roche Molecular Systems, Inc. HybProbe is a registered trademark of Roche Diagnostics GmbH. ROX is a trademark Life Technologies Corporation. Solaris is a registered trademark of Thermo Fisher Scientific Inc. Scorpions is a registered trademark of DxS, Ltd. of Manchester, UK.。