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天津医科大学实验动物使用审查表
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一、根据天津医科大学实验动物管理和使用委员会要求,2021年开始不在天津医科大学动物实验平台进行全程动物实验的不予审查,动物伦理审查收取审查费。
二、进驻动物实验平台进行实验前填写动物伦理审查表，该表代替动物实验申请书。
三、各种费用计算按填写内容进行核定，按规定执行。
四、按规定未交齐动物实验期间费用的不给予动物伦理审查结论。
五、仔细看申请书填写要求，并按要求详细填写内容。
六、动物实验期间设施兽医有权对动物实验进行检查，凡发现实验和申请书不符的情况，兽医有权终止实验，责令项目人补充或完善动物福利伦理审查。
七、实验动物使用和管理委员会审查意见页中项目基本情况由项目人填写，并应和申请书中的一致。
八、如果项目人对审查意见有英文需求，可在审查意见中参照现有格式提供中英对照。
九、动物伦理审查不接受电话咨询。
十、初审合格的申请表以“当前年月（如：202101）-项目名称-项目人-项目单位”格式提交到IACUC@邮箱。
未经初审合格不要提交。
十一、正式提交不提交填写说明页
天津医科大学实验动物使用审查表2021年动物实验版(代动物实验申请书)
实验动物使用和管理委员会审查意见（2021年动物实验版）
项目基本情况
编号：TMUaMEC 2021001。
天津医科大学的前身天津医学院创建于1951年，是新中国成立后国家政务院批准新建的第一所高等医学院校，著名内分泌学家、医学教育家朱宪彝教授为首任校长。
1993年，天津市市委、市政府决定天津医学院与天津第二医学院合并组建天津医科大学，同年12月得到国家教委批准。
1994年6月，天津医科大学正式组建成立。
学校现任党委书记为张连云教授，校长为著名生物化学与分子生物学家、中国科学院院士尚永丰教授。
学校为国家“211工程” 重点建设院校。
1医大简介天津医科大学是国家“211工程”重点建设院校，为仅有的两所进入国家“211工程”建设的独立设置西医院校（天津医科大学、北京协和医学院）之一。
在网大公布的2007年中国大学排名中天津医科大学在独立设置的75所医学院校中与北京中医药大学并列为第2名，综合排名为第67[2]名。
另外在网大2010年公布的1999年至2010年进步最显著的大学名单中，天津医科大学榜上有名，与中国政法大学、华东师范大学、苏州大学、西北大学、北京中医药大学这五所重点高校共同评为进步最显著的大学。
在武书连公布的2011年中国大学评价中，天津医科大学被评为2011中国大学医学最强的23所学校之一，等级为A级。
2011年6月1日上海交通大学高等教育研究院世界一流大学研究中心（教育部战略研究基地）校史板报(18张)完成的“中国两岸四地大学排名”中，在众多港澳台高校参评的情况下，天津医科大学综合排名列第67名，摘得中国内地独立设置医学院校中的第一。
2013年发布的中国·两岸四地大学排名中，在两岸四地高校中列第62位，大陆地区第37位。
[3]学校现有全日制本科以上在校生9894人，其中，本科生5329人，硕士生2871人，博士生428人，留学生1266人。
天津医科大学是继协和医学院后，国家最早批准试办八年制的医学院校之一，首批试办七年制的15所院校之一。
自建校以来，学校已培养了40000余名各层次医学人才。
英文缩略语注释ｄｉａｂｅｔｅｓｍｅｌｌｉｔｕｓ糖尿病ａｔｈｅｒｏｓｃｌｅｒｏｓｉＳ动脉粥样硬化ｐｒｏｓｔａｇｌａｎｄｉｎＥ前列腺素Ｅａｌｌｏｘａｎ四氧嘧啶ｓｍｏｏｔｈｍｕｓｃｌｅｃｅｌｌ平滑肌细胞ｅｘｔｒａｃｅｌｌｕｌａｒｍａｔｒｉｘ细胞外基质ｎｉｔｒｉｃｏｘｉｄｅ一氧化氮ｎｉｔｒｉｃｏｘｉｄｅｓｙｎｔｈｅｔａｓｅ一氧化氮合成酶ｅｎｄｏｔｈｅｌｉｎ内皮素ｐｒｏｓｔａｃｙｃｌｉｎ前列环素ｔｈｒｏｍｂｏｘａｎｅＡ２血栓烷素Ａ２ｔｉｓｓｕｅ—ｐｌａｓｍｉｎｏｇｅｎａｃｔｉｖａｔｏｒ组织型凝血酶原激活物ｐｌａｓｍｉｎｏｇｅｎａｃｔｉｖａｔｏｒｉｎｈｉｂｉｔｏｒ凝血酶原激活物抑制剂ｓｕｐｅｒｏｘｉｄｅｄｉＳｍｕｔａｓｅ超氧化物岐化酶ｌｉｐｉｄｐｅｒｏｘｉｄｅ脂质过氧化物ｏｘｉｄｉｚｅｄｌＯＷｄｅｎｓｉｔｙｌｉｐｏｐｒｏｔｅｉｎ氧化低密度脂蛋白ｇｌｙｃｏｓｙｌａｔｅｄＬＤＬ糖基化低密度脂蛋白ｇｌｙｃｏｘｉｄａｔｅｄ—ＬＤＬ糖基化氧化低密度脂蛋白ｃｙｃｌｉＣａｄｅｎｏｓｉｎｅｍｏｎｏｐｈｏｎｓｐｈａｔｅ环磷酸腺菅ｃｙｃ¨ｃｇｕａｎｉＦｉｅｍｏｎ。
ｐｈｏｎｓｐｈａｔｅ环磷酸鸟苷ｔｕｍｏｒｎｅｃｒｏｓｉＳｆａｃｆｏｒ肿瘤坏死因子ｉｎｔｅｒｌｅｕｋｉｎ白细胞介素ｃａｌｃｉｕｍｍｏｄｕｌｉｒｌ钙调素ｌ，一ａｒｇｊｎｉＤｅ左旋精氨酸。
●凹叱加，，曙ＥＸＣＭＳＩＡＰＩＤＯ—Ｖ。
Ｖ．ⅥⅥＦⅥ１附脶眦似㈣吲№懈盯哪ｍ忡呲㈣啪噼吣ｍ洲删肿儿吣㈨中文摘要目的：在以往实验的基础上，研究大剂量ＰＢＥ：及ＰＧＥ。
对糖尿病性动脉粥样硬化家兔病理形态学、血管内皮依赖性舒张功能、氧化应激、血凝纤溶状态及血栓形成的影响，探讨ＰＧＥ抗糖尿病性动脉粥样硬化的作用机理，从而为临床防治糖尿病大血管并发症提供科学依据。
｛方法：Ｉ．先采用静脉注射ＡＬＸ法复制ＤＭ模型，再饲以高胆固醇饲料复制ＤＭ性ＡＳ家兔模型，按血脂血糖水平随机分成３组。
２．利用大体和光镜标本观察ＰＧＥ对ＤＭ性Ａｓ家兔主动脉病变的影响。
糖尿病牙周炎昆明系模型小鼠牙周组织中脂联素的表达赵博;王永兰【摘要】BACKGROUND:Adiponectin and periodontal disease are closely related with type 2 diabetes. OBJECTIVE:To detect the expression of adiponectin in periodontal tissue of Kunming mice of diabetes periodontitis, and explore the effects on periodontal tissue pathology and prognosis. METHODS:Sixty 3-week-old Kunming mice were divided into three groups according to the different modeling methods:control group (normal feeding), diabetes group (diabetic model was established by injection of al oxan), diabetes and periodontitis group (periodontitis model was established by local ligation and bacteria developed). Each group contained 20 rats. RESULTS AND CONCLUSION:At 20 days after modeling, the blood glucose, fasting insulin and insulin resistance index of mice in the diabetes group were higher than that in the control group (P<0.05). ELISA, RT-PCR and western blot analysis results showed that, adiponectin gene and protein expression levels in periodontal tissues were the highest in the control group, then in the diabetes group, and the lowest in the diabetes and periodontitis group, at 1, 3, 6, months postoperatively, respectively (P<0.05). The periodontal gum tissue inflammation and histopathological changes were observed at 1, 3, 6, months postoperatively. The results showed that, the inflammation in periodontal tissues was the most obvious in the diabetes and periodontitis group, and was visible in the diabetes group, but the inflammation was not present inthe control group. Experimental findings indicate that, the down-regulation of adiponectin expression in the periodontal tissue is one of the important factors that trigger and aggravate the onset of periodontitis.%背景：脂联素与牙周炎和2型糖尿病发病有密切关系。
缺血预适应对肝脏热缺血再灌注损伤保护作用的机制研究金涛;胡宇;刘超【摘要】Objective To explore the pathogenesis of ischemic preconditioning to warm ischemia reperfusion injury of hepatocytes in rats. Methods Ninety SD rats were randomly divided into three experimental groups:sham operation group (group A), warm hepaticischemia/reperfusion group(group B and group C). Group C was given ischemic preconditioning treatment. Serum levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were detected 0 h, 2 h, 6 h, 12 h and 24 h after ischemia reperfusion injury. Levels of TNF-α and IL-1β were tested detected by ELISA. Levels of malondialdehyde (MDA) and superoxide dismutase (SOD) of hepatocytes were detected at the same time points. Mitochondrial membrane potential was examined to assess ischemia reperfusion injury of hepatocytes in rats using chart of intensity of JC-1 in mitochondria. Results The serum levels of ALT, TNF-α, IL-1β, and MDA were significantly higher in hepatic warm ischemia reperfusion group and ischemic preconditioning group than those in sham operation group (P<0.05). Values of prothrombin activity and cholinesterase were significantly lower in liver warm ischemia reperfusion group and ischemic preconditioning group than those of sham operation group (P<0.05). The SOD level of liver was significantly lower in warm ischemia reperfusion group and ischemic preconditioning group than that in sham operation group. The indexes were better in ischemicpreconditioning group than those of warm ischemia reperfusion group(P<0.05). The mitochondrial membrane potential level of liver cells reached the lowest value 0 hours after ischemia and reperfusion, and then increased gradually within 24 hours (P<0.05). And the level of mitochondrial membrane potential of liver cells was significantly higher in ischemic preconditioning group than that in warm ischemia reperfusion group (P<0.05). Conclusion Ischemic preconditioning may play a protective role in warm ischemia-reperfusion injury in rats. Ischemic preconditioning may significantly decrease the levels of ALT, AST, TNF-α,IL-1βand MDA, and increase the SOD activity in hepatocytes. Thedamageof mitochondrial membrane potential is decreased after ischemic preconditioning, which might be the pathogenesis of ischemic preconditioning to warm ischemia reperfusion injury of hepatocytes in rats.%目的：探讨缺血预适应对肝脏热缺血再灌注损伤保护作用的分子机制。
唑来膦酸局部应用对大鼠拔牙创软硬组织的影响崔彩雯;张健;马婷;汪铭【摘要】Objective: To investigate the effects of locally application of zoledronic acid (ZA) on hard and soft tissue in rats that were pretreated with ZA during tooth extraction. Methods: Fifty-four 7-week old male Wistar rats were divided into ZA group, gelatin sponge group, and blank control group, hi each group, left mandibular first molars were extracted under general anesthesia. Rats were euthanized two weeks after tooth extraction, and mandibular bones were collected. Bone mor-phometric analysis using X-ray images was performed and the gingival around the extraction socket was harvested for HE staining and Masson staining to observe the histopathology effect. Results: New bone formation in the extraction socket was promoted and alveolar ridge absorption was delayed in the ZA group compared to the control group. The alveolar ridges of the ZA group were higher than those of the control group (2.60± 0.l0mmVSl .99± 0.22mm, P0.001; 2.60± 0.10mm VS 2.12± 0.24mm, PO.001). Histological images in ZA group showed that the mucous about extraction socket was getting thinner, the fiber was slender and disordered, but no differences of blood ressels were observed. Conclusions: Local application of zoledronic acid could inhibit bone resorption and promote new bone formation of the tooth extraction socket, also it might inhibit the formation of collagen fibers. These actions of ZA may be relevant to the pathogenesis of BRONJ.%目的:观察唑来膦酸(zoledronic acid,ZA)局部应用对大鼠下颌磨牙拔除后剩余软硬组织的影响.方法:将54只雄性Wistar大鼠随机分为三组,即空白对照组、明胶海绵组和唑来膦酸治疗组,麻醉下完整拔除左侧下颌第一磨牙并做相应处理,术后2周同期处死所有大鼠.用X线片测量大鼠拔牙窝剩余牙槽嵴的高度,取拔牙窝周围牙龈组织制作石蜡切片行HE染色和Masson染色,观察大鼠下颌磨牙拔牙窝的愈合情况.结果:唑来膦酸治疗组牙槽嵴高度显著高于空白对照组和明胶海绵组(2.60±0.10mmVS1.99±0.22mm,P＜0.001;2.60±0.10mm VS 2.12±0.24mm,P＜0.001).组织学观察拔牙创周围黏膜层上皮变薄,胶原较为纤细且排列疏松,血管变化不明显.结论:唑来膦酸局部应用抑制牙槽嵴骨吸收,促进拔牙窝新骨形成,对软组织作用不显著,可能抑制牙龈黏膜中胶原纤维的合成.【期刊名称】《口腔颌面修复学杂志》【年(卷),期】2011(012)005【总页数】4页(P261-264)【关键词】唑来膦酸;局部用药;牙槽骨;软组织【作者】崔彩雯;张健;马婷;汪铭【作者单位】天津医科大学口腔医院颌面外科天津300070;天津医科大学口腔医院颌面外科天津300070;天津医科大学口腔医院颌面外科天津300070;南京市中西医结合医院江苏210000【正文语种】中文【中图分类】R782.11双磷酸盐(bisphosphonate，BP)用于治疗骨代谢异常类疾病和恶性肿瘤的骨吸收抑制剂，有报道[1]发现BP治疗的癌症患者拔牙后发生下颌骨坏死(bisphosphonate-related osteonecrosisof thejaw s，BRONJ)其中以唑来膦酸所占比例高。
骨髓间充质干细胞移植脑梗死模型后的CT、MRI特征分析杨廷双【摘要】背景：研究表明骨髓间充质干细胞可通过多种途径发挥促进脑组织功能恢复的作用。
目的：分析骨髓间充质干细胞移植脑梗死模型大鼠的CT、MRI特征。
<br> 方法：将40只脑梗死模型大鼠随机分为2组，梗死组通过尾静脉注射1 mL PBS、移植组通过尾静脉注射1 mL细胞浓度为2.0×109 L-1的细胞悬液。
于移植后1，2，3周进行神经功能缺损评分(mNSS)，评价神经功能恢复情况；于移植后的6 h，1，3，5，7 d对各组大鼠行CT及MRI扫描，观察脑梗死区TIWI、T2WI、FLAIR、DWI序列的信号改变特征，梗死体积大小。
测量脑梗死区域T1WI、T2WI、FLAIR、DWI序列的信号强度比(SIR)及其相对变化率(∆SIR)，并与正常对侧相应解剖区域进行比较。
<br> 结果与结论：移植后1，2，3周，移植组的神经功能缺损评分明显低于梗死组(P<0.05)；移植后3，5，7 d时移植组脑梗死体积较梗死组显著减少(P<0.05)，移植组T1WI序列SIR均明显高于梗死组，T2WI、FLAIR序列SIR较梗死组显著降低，差异有显著性意义(P<0.05)；移植后7 d时移植组DWI序列SIR较梗死组明显降低(P<0.05)。
移植组T1WI序列∆SIR与梗死组比较差异无显著性意义(P>0.05)，移植组T2WI、FLAIR及DWI序列∆SIR较梗死组显著增高，差异有显著性意义(P<0.05)。
结果显示MRI可显示大脑任意角度的切面像，对骨髓间充质干细胞移植治疗脑梗死的疗效评价起重要作用。
%BACKGROUND:Studies have shown that bone marrow mesenchymal stem cel s can play a catalytic role in brain function recovery through a variety of ways. OBJECTIVE:To analyze the CT and MRI features of rats with cerebral infarction undergoing bone marrow mesenchymal stem cel transplantation. METHODS:Forty rats with cerebral infarction wererandomized into two groups:model group was treated with 1 mL PBS and transplantation group was injected with 2.0×109/L cel suspension. Modified neurological severity score was detected at 1, 2, 3 weeks after transplantation for neurological function evaluation. CT and MRI scans were performed at 6 hours, 1, 3, 5, 7 days after transplantation to observe the changing features of T1WI, T2WI, FLAIR, DWI sequences in the infracted area as wel as infarction size. Signal intensity ratios (SIR) of T2WI, FLAIR, DWI sequences and relative rates of change (∆SIR) were determined and compared with the normal values. RESULTS AND CONCLUSION:After transplantation 1, 2 and 3 weeks, the modified neurological severity scores were significantly lower in the transplantation group than the model group (P<0.05). After transplantation 3, 5, 7 days, the infracted size was reduced significantly in the transplantation group compared with the model group (P<0.05). Compared with the model group, SIR value of T1WI sequence was higher, but the SIR values of T2WI and FLAIR sequences were lower in the transplantation (P<0.05). There were significant differences in the∆SIR values of T2WI, FLAIR and DWI (P<0.05) rather than T1WI (P>0.05) between the two groups. These findings indicate that MRI can show the brain sections at any angle, and play an important role to assess the therapeutic efficacy of bone marrow mesenchymal stem cel transplantation on cerebral infarction.【期刊名称】《中国组织工程研究》【年(卷),期】2015(000)036【总页数】5页(P5795-5799)【关键词】干细胞;移植;骨髓间充质干细胞;大鼠;脑梗死;CT;MRI;特征分析;细胞移植【作者】杨廷双【作者单位】天津医科大学总医院滨海医院放射科，天津市 300480【正文语种】中文【中图分类】R394.2文章亮点：1骨髓间充质干细胞移植对恢复脑梗死大鼠神经功能，减轻脑组织水肿和脑梗死体积起到促进作用。
