Streptozocin_18883-66-4_DataSheet_MedChemExpress

Pierce Biotechnology 3747 N. Meridian Road PO Box 117 Rockford, lL 61105 USA (815) 968-0747 (815) 968-tin is a small naturally occurring vitamin that binds with high affinity to avidin and streptavidin proteins. Biotinylated proteins typically retain biological activity because the biotin group is relatively small. An antibody conjugated with several biotin molecules can amplify signal, thereby increasing the sensitivity of many assays. The bond formation between biotin and avidin is rapid and, once formed, is unaffected by most extremes of pH, organic solvents and other denaturing agents. Labeled proteins can be purified using immobilized streptavidin, avidin or NeutrAvidin™ Gel (see Related Thermo Scientific Products) and detected in ELISA, dot blot or Western blot applications. N-Hydroxysuccinimide (NHS) esters are the most commonly used biotinylation reagents. In pH 7-9 buffers, NHS esters react efficiently with primary amino groups (-NH2) by nucleophilic attack, forming an amide bond and releasing the NHS (Figure 1). Proteins typically have many sites for labeling, including the primary amine in the side chain of lysine (K) residues and the N-terminus of each polypeptide.

Material Safety Data SheetCopyright, 2005, 3M Company. All rights reserved. Copying and/or downloading of this information for the purpose of properly utilizing 3M products is allowed provided that: (1) the information is copied in full with no changes unless prior written agreement is obtained from 3M, and (2) neither the copy nor the original is resold or otherwise distributed with the intention of earning a profit thereon.This material safety data sheet (MSDS) is provided as a courtesy in response to a customer request. This product is not regulated under, and a MSDS is not required for this product by the OSHA Hazard Communication Standard (29 CFR 1910.1200) because, when used as recommended or under ordinary conditions, it should not present a health and safety hazard. However, use or processing of the product not in accordance with the product's recommendations or not under ordinary conditions may affect the performance of the product and may present potential health and safety hazards.SECTION 1: PRODUCT AND COMPANY IDENTIFICATIONPRODUCT NAME: Scotch brand No. 8663 Polyurethane Protective Tape; SJ8665 and SJ8667HS RadomeBoots or 8673 ClearMANUFACTURER: 3MDIVISION: Aerospace Aircraft Maint DivisionADDRESS: 3M CenterSt. Paul, MN 55144-1000EMERGENCY PHONE: 1-800-364-3577 or (651) 737-6501 (24 hours)Issue Date: 09/13/2005Supercedes Date: 11/17/2004Document Group: 07-1862-7SECTION 2: INGREDIENTSIngredient C.A.S. No.% by WtPOLYURETHANE Unknown 60 - 100ACRYLIC ACID-ISOOCTYL ACRYLATE POLYMER 9017-68-9 7 - 13SECTION 3: HAZARDS IDENTIFICATION3.1 EMERGENCY OVERVIEWSpecific Physical Form: Roll of TapeOdor, Color, Grade: CLEARGeneral Physical Form: SolidImmediate health, physical, and environmental hazards: This product, when used under reasonable conditions andin accordance with the 3M directions for use, should not present a health hazard. However, use or processing of the product in a manner not in accordance with the product's directions for use may affect the performance of the product and may present potential health and safety hazards.3.2 POTENTIAL HEALTH EFFECTSEye Contact:No health effects are expected.Skin Contact:No health effects are expected.Inhalation:No health effects are expected.Ingestion:No health effects are expected.SECTION 4: FIRST AID MEASURES4.1 FIRST AID PROCEDURESThe following first aid recommendations are based on an assumption that appropriate personal and industrial hygiene practices are followed.Eye Contact: Flush eyes with large amounts of water. If signs/symptoms persist, get medical attention.Skin Contact: Wash affected area with soap and water. If signs/symptoms develop, get medical attention.Inhalation: No need for first aid is anticipated.If Swallowed: No need for first aid is anticipated.SECTION 5: FIRE FIGHTING MEASURES5.1 FLAMMABLE PROPERTIESAutoignition temperature Not ApplicableFlash Point Not ApplicableFlammable Limits - LEL Not ApplicableFlammable Limits - UEL Not ApplicableOSHA Flammability Classification: Not Applicable5.2 EXTINGUISHING MEDIAUse fire extinguishers with class B extinguishing agents (e.g., dry chemical, carbon dioxide).5.3 PROTECTION OF FIRE FIGHTERSSpecial Fire Fighting Procedures: Wear full protective equipment (Bunker Gear) and a self-contained breathing apparatus(SCBA).Unusual Fire and Explosion Hazards: Not applicable. No unusual fire or explosion hazards are anticipated.Note: See STABILITY AND REACTIVITY (SECTION 10) for hazardous combustion and thermal decomposition information.SECTION 6: ACCIDENTAL RELEASE MEASURESAccidental Release Measures: Not applicable.SECTION 7: HANDLING AND STORAGE7.1 HANDLINGAvoid prolonged or repeated skin contact. This product is considered to be an article which does not release or otherwise result in exposure to a hazardous chemical under normal use conditions.7.2 STORAGENot applicable.SECTION 8: EXPOSURE CONTROLS/PERSONAL PROTECTION8.1 ENGINEERING CONTROLSNot applicable.8.2 PERSONAL PROTECTIVE EQUIPMENT (PPE)8.2.1 Eye/Face ProtectionNot applicable.8.2.2 Skin ProtectionNot applicable. Gloves not normally required. Avoid prolonged or repeated skin contact.8.2.3 Respiratory ProtectionUnder normal use conditions, airborne exposures are not expected to be significant enough to require respiratory protection.8.2.4 Prevention of SwallowingNot applicable.8.3 EXPOSURE GUIDELINESNone EstablishedSECTION 9: PHYSICAL AND CHEMICAL PROPERTIESSpecific Physical Form: Roll of TapeOdor, Color, Grade: CLEARGeneral Physical Form: SolidAutoignition temperature Not ApplicableFlash Point Not ApplicableFlammable Limits - LEL Not ApplicableFlammable Limits - UEL Not ApplicableBoiling point Not ApplicableDensity 1.15 g/cm3 [@ 20 ºC]Vapor Density Not ApplicableVapor Pressure Not ApplicableSpecific Gravity 1.15 [Ref Std: WATER=1]pH Not ApplicableMelting point No Data AvailableSolubility In Water Not ApplicableSolubility in Water NilEvaporation rate Not ApplicableHazardous Air Pollutants 0 % weight [Test Method: Calculated]Volatile Organic Compounds 0 % [Test Method: calculated per EPA method 24]Percent volatile 0 % [Test Method: Estimated]VOC Less H2O & Exempt Solvents 0 g/l [Test Method: calculated per EPA method 24]Viscosity Not ApplicableSECTION 10: STABILITY AND REACTIVITYStability: Stable.Materials and Conditions to Avoid: None knownHazardous Polymerization: Hazardous polymerization will not occur.Hazardous Decomposition or By-ProductsSubstance ConditionCarbon monoxide During CombustionCarbon dioxide During CombustionIrritant Vapors or Gases During CombustionHazardous Decomposition: Under recommended usage conditions, hazardous decomposition products are not expected. Hazardous decomposition products may occur as a result of oxidation, heating, or reaction with another material.SECTION 11: TOXICOLOGICAL INFORMATIONPlease contact the address listed on the first page of the MSDS for Toxicological Information on this material and/or itscomponents.SECTION 12: ECOLOGICAL INFORMATIONECOTOXICOLOGICAL INFORMATIONNot applicable.CHEMICAL FATE INFORMATIONNot applicable.SECTION 13: DISPOSAL CONSIDERATIONSWaste Disposal Method: Dispose of waste product in a sanitary landfill. As a disposal alternative, incinerate in an industrial or commercial facility.Since regulations vary, consult applicable regulations or authorities before disposal.SECTION 14:TRANSPORT INFORMATIONID Number(s):70-0000-4526-3, 70-0000-5607-0, 70-0000-6236-7, 70-0000-6237-5, 70-0000-8242-3, 70-0000-8400-7, 70-0061-2880-8, 70-0061-7863-9, 70-0061-7864-7, 70-0061-7865-4, 70-0061-7866-2, 70-0062-6990-9, 70-0062-6991-7, 70-0702-5833-3, 70-0702-5834-1, 70-0703-0504-3, 70-0703-7786-9, 70-0704-2776-3, 70-0707-0642-2, 70-0707-0643-0, 70-0709-1393-7, 70-0709-1394-5, 70-0709-1395-2, 70-0709-1396-0, 70-0709-1397-8, 70-0709-1398-6, 70-0709-1401-8, 70-0709-1402-6, 70-0709-1446-3, 70-0709-1447-1, 70-0709-1448-9, 70-0709-1449-7, 70-0709-1450-5, 70-0709-1451-3, 70-0709-1452-1, 70-0709-1453-9, 70-0709-1454-7, 70-0709-1455-4, 70-0709-1456-2, 70-0709-1457-0, 70-0709-1458-8, 70-0709-1459-6, 70-0709-1460-4, 70-0709-1461-2, 70-0709-1462-0, 70-0709-1463-8, 70-0709-1476-0, 70-0709-1477-8, 70-0709-1486-9, 70-0709-1487-7, 70-0709-1488-5, 70-0709-1489-3Please contact the emergency numbers listed on the first page of the MSDS for Transportation Information for this material.SECTION 15: REGULATORY INFORMATIONUS FEDERAL REGULATIONSContact 3M for more information.311/312 Hazard Categories:Fire Hazard - No Pressure Hazard - No Reactivity Hazard - No Immediate Hazard - No Delayed Hazard - NoSTATE REGULATIONSContact 3M for more information.CHEMICAL INVENTORIESThis product is an article as defined by TSCA regulations, and is exempt from TSCA Inventory listing requirements.Contact 3M for more information.INTERNATIONAL REGULATIONSContact 3M for more information.This MSDS has been prepared to meet the U.S. OSHA Hazard Communication Standard, 29 CFR 1910.1200.SECTION 16: OTHER INFORMATIONNFPA Hazard ClassificationHealth: 0 Flammability: 0 Reactivity: 0 Special Hazards: NoneNational Fire Protection Association (NFPA) hazard ratings are designed for use by emergency response personnel to address the hazards that are presented by short-term, acute exposure to a material under conditions of fire, spill, or similar emergencies. Hazard ratings are primarily based on the inherent physical and toxic properties of the material but also include the toxic properties of combustion or decomposition products that are known to be generated in significant quantities.Revision Changes:Copyright was modified.Section 9: Property description for optional properties was modified.DISCLAIMER: The information in this Material Safety Data Sheet (MSDS) is believed to be correct as of the date issued. 3M MAKES NO WARRANTIES, EXPRESSED OR IMPLIED, INCLUDING, BUT NOT LIMITED TO, ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE OR COURSE OF PERFORMANCE OR USAGE OF TRADE. User is responsible for determining whether the 3M product is fit for a particular purpose and suitable for user's method of use or application. Given the variety of factors that can affect the use and application of a 3M product, some of which are uniquely within the user's knowledge and control, it is essential that the user evaluate the 3M product to determine whether it is fit for a particular purpose and suitable for user's method of use or application.3M provides information in electronic form as a service to its customers. Due to the remote possibility that electronic transfer may have resulted in errors, omissions or alterations in this information, 3M makes no representations as to its completeness or accuracy. In addition, information obtained from a database may not be as current as the information in the MSDS available directly from 3M.3M MSDSs are available at 。

432食品与药品Food and Drug2020年第22卷第6期苯磺贝他斯汀滴眼液中苯扎氯钱含量分析方法研究方夏琴，檀华进，靳雯臻，姜栋，谭晓川，张宇佳，郑稳生*(北京协和医学院，中国医学科学院药物研究所药物传输技术及新型制剂北京市重点实验室，北京100050)摘要：目的建立苯磺贝他斯汀滴眼液中抑菌剂苯扎氯钱含量测定的高效液相色谱法(HPLC),并分析滤膜吸附作用对检测结果的影响。

方法色谱柱为Agilent TC-C C18(4.6mmx250mm,5pn),流动相为乙ffl-5mmol/L乙酸皱溶液(含1%三乙胺，用磷酸调节pH值至5.0)=65:35,检测波长214nm,进样量20pl；通过控制滤膜材质、来源、过滤体积，考察滤膜吸附差异。

结果溶剂与辅料对苯扎氯镀含量测定无干扰，在0.02438-0.07314mg/ml范围内线性关系良好(尸2.453^)W173^^，仪器精密度RSQ为0.44%,重复性RSD为1.25%,中间精密度RSQ为1.70%,低、中、高浓度对应的平均回收率为96.94%,RSD为1.28%,24h 内溶液稳定性RSD为0.81%,3批检测含量：112.89%,104.61%,108.22%。

滤膜材质、来源、过滤体积均会影响滴眼液中苯扎氯镀吸附情况，不同同系物吸附强度不同，且亲水性滤膜吸附作用相对较小。

结论该方法准确可靠，灵敏度较高，适用于对苯磺贝他斯汀滴眼液中苯扎氯镀的检查。

关键词：高效液相色谱；苯磺贝他斯汀滴眼液；苯扎氯钱；抑菌剂；含量测定中图分类号：R927.2文献标识码：A文章编号：1672-979X(2020)06-0432-05DOI：10.3969/j.issn.l672-979X.2020.06.003Determination of Benzalkonium Chloride in Bepotastine Besilate Eye DropsFANG Xia-qin,TAN Hua-jin,JIN Wen-zhen,JIANG Dong,TAN Xiao-chuan,ZHANG Yu-jia,ZHENG Wen-sheng (Beijing Key laboratory of D rug Delivery Technology and Novel Formulation,Institute of M ateria Medica,Chinese Academy of M edical Sciences&Peking Union Medical College,Beijing100050,China)Abstract:Objective To establish an HPLC method for the determination of the bacteriostatic agent benzalkonium chloride in Bepotastine Besilate Eye Drops,and to analyze the effect of filter adsorption on the detection results. Methods An Agilent TC-C C*(4.6mmx250mm,5pm)column was used with the mixture of acetonitrile and5 mmol/L ammonium acetate solution(containing1%triethylamme,adjusted to pH5.0with phosphoric acid)(65:35) as the mobile phase.The detection wavelength was214nm and the injection volume was20pl.The filter adsorption difference was investigated by controlling the filter material,source and filtration volume.Results Solvents and excipients did not interfere with the determination of benzalkonium chloride.The linear relationship was good in the range of0.02438-0.07314mg/ml y2.453x10?卄2.173X104).The precision RSD of the instrument was0.44%,theTtSZ) of repeatability was1.25%,and the intermediate precision RSD was1.70%.The average recovery of low,medium and high concentrations was96.94%,with RSD of1.28%,The RSD of stability was0.81%within24h and the收稿日期：2020-03-06基金项目：十三五“重大新药创制”科技重大专项(2018ZX09721003-008-030)；中国医学科学院医学与健康科技创新工程(No.2017-I2M-1-011)作者简介：方夏琴，助理研究员，研究方向：药物分析研究Tel:************E-mail:*****************水通讯作者：郑稳生，研究员，博士，博士生导师，研究方向：中、西药物新制剂及新释药系统技术研究与开发Tel:010­63165233E-mail:187****************食品与药品Food and Drug2020年第22卷第6期433contents of3batches of preparations were112.89%,104.61%and108.22%,respectively.The material,source and filtration volume of the membrane could all affect the adsorption of benzalkonium chloride in Bepotastine Besilate Eye Drops.The adsorption strength of different homologues was different and the adsorption of hydrophilic membrane was relatively small.Conclusion The method is accurate,reliable and sensitive,and is suitable for the detection of benzalkonium chloride in Bepotastine Besilate Eye Drops.Key Words:HPLC;Bepotastine besilate Eye Drops;benzalkonium chloride;preservative;determination苯磺贝他斯汀（bepotastine besilate）是一种组胺受体拮抗剂，属于第二代Hl抗组胺药，主要特点是使用后起效迅速，具有良好的临床效果与安全性2〕。

0910.06479774001ቢ

The recommended starting concentration is a 3:1. For most cell types, these X-tremeGENE HP DNA Transfection Reagent to DNA ratios provide excellent transfection efficiency. Further optimization may increase transfection efficiency in your particular application. In addition to varying the ratio, other parameters may also be evaluated, such as the amount of transfection complex added. For additional optimization guidelines, see Section 3, Troubleshooting and visit . Plasmid DNA • For best results, accurately determine the plasmid DNA concentration using 260-nm absorption; estimates of DNA by measuring gel band density are not recommended. Determine DNA purity using a 260 nm/280 nm ratio (the optimal ratio is 1.8). • Prepare the plasmid DNA solution using sterile TE (Tris/EDTA) buffer or sterile water at a concentration of 0.1 to 2.0 µg/µl. • Use high quality DNA preparation kits to obtain endotoxin-free DNA. Cell Culture Conditions • Minimize intra- and inter-experimental variance in transfection efficiency using cells that are regularly passaged, proliferating well in a log-growth phase, and plated at a consistent density. • For best results, accurately quantify cell concentration using a hematocytometer or automated system. • Cells must be healthy and free of Mycoplasma. • Cells should have a low passage number to achieve best results. Other Media Additives In some cell types, antimicrobial agents (e.g., antibiotics and fungicides) commonly included in cell-culture media may adversely affect the transfection efficiency of X-tremeGENE HP DNA Transfection Reagent. If possible, exclude additives in initial experiments. Once high-efficiency conditions have been established, these components can be added back while monitoring transfection results. Cell growth and/or transfection efficiency may be affected by variations in serum quality and medium formulations. Verification of Vector Function Optimize transfection conditions using a known positive-control reporter gene construct before transfecting cells with a new vector construct: • Determine transfection efficiency using a reporter gene assay, such as ␤-Gal*, Luciferase*, or SEAP*. • Sequence flanking vector insert regions to verify the integrity of your new construct. 2.2 Transfection Procedure Adherent Cells: Plate cells approximately 24 hours before transfection making sure cells are at the optimal concentration in the appropriate cell culture vessel. Suspension Cells: Plate freshly passaged cells at optimal concentration.