本刊常用计量单位表示法
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Optimization and Primers Screening of MSAP Analysis System in Cucurbita JIANG Yi，JIANG Li-na*，ZHOU Jun-guo*
（School of Horticulture and Landscape Architecture，Henan Institute of Science and Technology，Horticultural Plant Resources Utilization and Germplasm Enhancement Engineering Research Center of Henan Province，Xinxiang 453003，Henan，China）Abstract： Taking pumpkin ‘Beiguan’（Cucurbita maxima） as material，this experiment used orthogonal design L16（45） to optimize main factors of pumpkin MSAP pre-amplification and selective amplification system．The results showed that in the first step：the best digestion time was 2 hours，HapⅡ（HpaⅡ，MspⅠ）dosage was 0.5 μL；in the second step：digestion time was 6 hours，Eco RⅠdosage was 0.4 μL．The digest-ligation system，including 21 μL digestion product，1 μL Eco R Ⅰcontact，and 1 μL HapⅡ contact．The connection time was 12 hours．The T4 DNA Ligase usage was 0.5 μL．The optimal pre-amplification reaction system contained 2.0 μL digest-ligation products，1.5 μL 10 × PCR Buffer（Mg2+ plus），2.0 μL dNTP（2.5 mmol · L-1），0.6 μL Taq enzyme（5 U · μL -1），0.4 μL upstream and downstream primers（10 μmol · L-1）．The optimal selective amplification reaction system was template 4 μL diluted 120 times from the pre-amplification products．The rest was identical with pre-amplification system．Finally，36 pairs of primers suitable for pumpkin MSAP analysis were screened out and verified with 6% PAGE by the established MSAP system．The result indicated that the optimized MSAP system had good polymorphism，the system was stable and repeatable．This experiment has laid a foundation for subsequent MSAP analysis on pumpkin．Key words：Cucurbita；MSAP；System optimization；Primer screening
本刊常用计量单位表示法
1. 时间：用a（年）、d（天）、h（小时）、min（分）、s（秒）表示。
2. 面积：用km2（平方千米）、hm2（公顷）、m2（平方米）、cm2（平方厘米）表示，不用亩，可暂用667 m2代替。
3. 质量（原为重量）：用g（克）、kg（千克）、t（吨）表示。
4. 浓度：可用%表示质量分数和体积分数。
质量浓度用kg·L-1 （千克每升）、g·L-1（克每升）、mg·L-1（毫克每升）、μg·L-1（微克每升）表示。
ppm并非单位符号，不能使用，可根据具体
情况改写成质量分数mg·kg-1、体积分数μL·L-1或质量浓度 mg·L-1，数值保持不变。
5. 组合单位：
① 组合单位中不能插入其他信息，如“VC含量25 mg/100 g 鲜重”，应为“VC含量250 mg·kg-1（鲜样质量）”；“施肥量140 kg N/hm2”应为“施N肥量140 kg·hm-2”。
② 组合单位书写错误，如“mg/kg·d”，应写为“mg·kg-1·d-1”。
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