For You Tec company

Title: Embracing Innovation: A Glimpse into Our Company's StoryIn the heart of technological advancement and innovation, our company stands tall, a beacon of excellence and creativity. Since our establishment, we have been committed to delivering unparalleled products and services, tailored to meet the evolving needs of our clients.Our journey began with a vision: to harness the power of technology and transform it into solutions that make lives easier, more efficient, and richer. Over the years, we have grown from a small startup to a globally recognized brand, with a diverse portfolio of offerings that span across multiple industries.At the core of our operations is a team of highly skilled professionals, passionate about what they do and dedicated to exceeding expectations. Our research and development team is constantly on the lookout for new trends and technologies, ensuring that our products remain at the forefront of innovation.Our commitment to quality is unwavering. We adhere to strict standards and protocols, ensuring that every product we release is of the highest caliber. This dedication to excellence has earned us numerous awards and accolades, further strengthening our position as a leader in the industry.But we are not content with resting on our laurels. We believe that true success lies in continuous improvement and growth. Therefore, we invest heavily in training and development, encouraging our employees to explore new ideas and push the boundaries of what is possible.In this宣传册, you will find a comprehensive overview of our company, including our mission, values, and the range of products and services we offer. You will also get a glimpse into our culture, our people, and the stories that make us unique.We invite you to join us on this journey of discovery, as we embark on new adventures and create the future together. Thank you for considering our company, and we look forward to the opportunity to serve you.。

A .English to Chinese1.随着我国加入WTO和对外经济交往迅速加深与扩大，英语广告已逐渐发展成为企业增进出口销售，开拓海外市场的一个主要手腕。

2.广告可以利用有争议的政治话题、社会话题作由头宣传自己，也可以赤裸裸吹自己的产品以抓住消费者的注意力，引导他们购买产品或服务。

3.这种牌子的吸尘器在国际市场上颇具竞争力，是同类产品中最畅销的。

4.咱们生产的电脑其特点是品质好，体积小，节能，而且易学好用。

5.随着世界经济的发展，报纸，杂志和电视愈来愈依赖广告，并使之成为公司收入的一大部份。

1. Along with China's entry into the WTO and more businesses going international, English advertisement has gradually become a major tool for enterprises to promote exportations and to develop foreign markets.2. Advertising can incorporate controversial political tones, social tones, partial nudity, etc. to capture the attention of consumers and entice them to make a purchase.3. Vacuum cleaner of this brand is competitive in the international market and it is the best- selling product of the same kind.4. The computer we produced is characterized by its high quality, compact size, energy saving qualities and is also easy to operate.5. With the rapid development of world economy, newspapers, magazines and television companies are dependent on advertising for a large part of their income.B. Chinese to English1.If the advertisers simply translate the successful ads at home into English, it is quite likely that they will not succeed.2.One of the most important automotive shows in China, Auto China 2021 will be held in June at the China International Exhibition Centre, Beijing.3.Interactive TV movies television into an entirely new medium combining entertainment, shopping, marketing and advertising.4.Advertising on British television is subject to strict regulations.5.As our product has all the features you need and is 20% cheaper compared with that of Japanese make,I strongly recommend it to you.1．若是广告的写作者只是简单地把国内成功的广告翻译成英文，往往不会成功。

汉翻英1.我公司是该地区经营电子产品的主要进口商之一。

我们借此机会与贵方接洽，希望与贵方建立贸易关系。

Our company is one of the leading importers of electronic products in the region, we take thisopportunity to contact you, want to enter into business relations with you.2.希望我们的商品能够使你们满意，并能从你们那里获得更多的订单。

We hope that the goods can make you satisfied, and can get more orders from you.3.关于支付条件，我们通常要求保兑的、不可撤销的、凭即期汇票支付的信用证。

Regarding the terms of payment, we usually require confirmed, irrevocable letter of credit payableat sight.4.我们正在执行你方678号订单。

请相信我们定将在你方所规定的期限内安排装运。

We are running your 678 Order .please be assured that we will be specified in your shipment withinthe time limit stipulated by you.5.请将你方信用证6789号的船期及有效期分别延展至10月15号及10月30日。

Please send your letter of credit No. 6789 sailing date and term of validity were respectivelyextended to October 15 and October 30 .6.你方愿接受错发货物，致谢。

Unit61 Decision1.你需要时间考虑对方要求。

你说，你现在无法作出决定。

I can't make a deasion righL now.2.你们希望合作方尽快答复，你对他们说，请尽早告诉你方他们的决定。

Please' tell us your decision as soon as poSsitolc.3.你方提案还没有得到对方的答复，你问他，对于你们的提案他决定了吗？Have you made any decision on our proposal?4.外商在订货时犹豫不决，他说，请给你一个星期以便作出最后结论。

Please give me a couple of days to make a decision.5.你需要一周来考虑这一问题。

你对外商说，请给你一个星期以便作出最后结论。

I'd rather ask you to wait about a week for a final decislon.6.这个问题须由董事会商议。

你说，这个问题你没有最终决定权。

I don't have the power to make a flnal decision on the matter.7.客户提出要你方退还全部货款。

你为难地说，恐怕这个问题你没有权力解决。

I'm afraid I really can't make a decision about this by myself.8.你不能决定是否接受对方提出的价格，你说，你没有被给予价格的最终决定权。

I've not been given the power to make a final decision on the price.make a final decision 做出最终决定Unit62 Technology(一)1.你公司没有接收厂家提供的技术工人。

你说，他们的技术水平不高。

公司简介英文翻译ABC 公司是 XX 产品的生产商，拥有齐全的检测设备和雄厚的技术力量。

品种齐全，质量好，价格合理，外观精美，我们的产品广泛的应用于X (应用的行业领)和其他行业。

我们的产品已得到广大用户的一致认可和信赖，并能满足不断发展的经济和社会需求。

我们热诚欢迎各行各业的商友，期望与您建立友好合作的商务联系并达到双赢的目标。

对应英文介绍模板公司英文名称) is a manufacturer of (主营产品) with well-equipped testing facilities and strong technical a wide range, good quality, reasonable prices and stylish designs, our products are extensively used in应用的行业领域) and other products are widely recognized and trusted by users and can meet continuously changing economic and social wele new and old customers from all walks of life to contact us for future busine relationships and mutual succe!中文介绍模板XX公司地处XX专业生产ABC产品。

公司拥有一支经验丰富的专业队伍。

我们的产品出口到全世界很多国家和地区特别是XX。

产品深受客户的好评。

我们欢迎客户、商业协会以及来自世界各地的朋友联系我们寻求共赢的合作。

对应英文介绍模板 (公司英文名称) is located in (公司所在城市) and is specialized in manufacturing (主营产品).With an experienced and profeional team, we have exported our products to many countries and regions all over the world, especially (产品销往地).Our products enjoy a good reputation among our wele customers, busine aociations and friends from all parts of the world to contact us and seek cooperation for mutual benefits.中文介绍模板建于XX年， ABC公司致力于XX贸易。

Instruction ManualProBond TM Purification SystemFor purification of polyhistidine-containing recombinant proteinsCatalog nos. K850-01, K851-01, K852-01, K853-01, K854-01,R801-01, R801-15Version K2 September200425-0006iiTable of ContentsKit Contents and Storage (iv)Accessory Products (vi)Introduction (1)Overview (1)Methods (2)Preparing Cell Lysates (2)Purification Procedure—Native Conditions (7)Purification Procedure—Denaturing Conditions (11)Purification Procedure—Hybrid Conditions (13)Troubleshooting (15)Appendix (17)Additional Protocols (17)Recipes (18)Frequently Asked Questions (21)References (22)Technical Service (23)iiiKit Contents and StorageTypes of Products This manual is supplied with the following products:Product CatalogNo.ProBond™ Purification System K850-01ProBond™ Purification System with Antibodywith Anti-Xpress™ Antibody K851-01with Anti-myc-HRP Antibody K852-01with Anti-His(C-term)-HRP Antibody K853-01with Anti-V5-HRP Antibody K854-01ProBond™ Nickel-Chelating Resin (50 ml) R801-01ProBond™ Nickel Chelating Resin (150 ml) R801-15ProBond™Purification System Components The ProBond™ Purification System includes enough resin, reagents, and columns for six purifications. The components are listed below. See next page for resin specifications.Component Composition Quantity ProBond™ Resin 50% slurry in 20% ethanol 12 ml5X NativePurification Buffer250 mM NaH2PO4, pH 8.02.5 M NaCl1 × 125 ml bottleGuanidinium LysisBuffer6 M Guanidine HCl20 mM sodium phosphate, pH 7.8500 mM NaCl1 × 60 ml bottleDenaturingBinding Buffer8 M Urea20 mM sodium phosphate, pH 7.8500 mM NaCl2 × 125 ml bottlesDenaturing WashBuffer8 M Urea20 mM sodium phosphate, pH 6.0500 mM NaCl2 × 125 ml bottlesDenaturing ElutionBuffer8 M Urea20 mM NaH2PO4, pH 4.0500 mM NaCl1 × 60 ml bottleImidazole 3 M Imidazole,20 mM sodium phosphate, pH 6.0500 mM NaCl1 × 8 ml bottlePurificationColumns10 ml columns 6Continued on next pageivKit Contents and Storage, ContinuedProBond™Purification System with Antibody The ProBond™ Purification System with Antibody includes resin, reagents, and columns as described for the ProBond™ Purification System (previous page) and 50 µl of the appropriate purified mouse monoclonal antibody. Sufficient reagents are included to perform six purifications and 25 Western blots with the antibody.For more details on the antibody specificity, subclass, and protocols for using the antibody, refer to the antibody manual supplied with the system.Storage Store ProBond™ resin at +4°C. Store buffer and columns at room temperature.Store the antibody at 4°C. Avoid repeated freezing and thawing of theantibody as it may result in loss of activity.The product is guaranteed for 6 months when stored properly.All native purification buffers are prepared from the 5X Native PurificationBuffer and the 3 M Imidazole, as described on page 7.The Denaturing Wash Buffer pH 5.3 is prepared from the Denaturing WashBuffer (pH 6.0), as described on page 11.Resin and ColumnSpecificationsProBond™ resin is precharged with Ni2+ ions and appears blue in color. It isprovided as a 50% slurry in 20% ethanol.ProBond™ resin and purification columns have the following specifications:• Binding capacity of ProBond™ resin: 1–5 mg of protein per ml of resin• Average bead size: 45–165 microns• Pore size of purification columns: 30–35 microns• Recommended flow rate: 0.5 ml/min• Maximum flow rate: 2 ml/min• Maximum linear flow rate: 700 cm/h• Column material: Polypropylene• pH stability (long term): pH 3–13• pH stability (short term): pH 2–14ProductQualificationThe ProBond™ Purification System is qualified by purifying 2 mg of myoglobinprotein on a column and performing a Bradford assay. Protein recovery mustbe 75% or higher.vAccessory ProductsAdditionalProductsThe following products are also available for order from Invitrogen:Product QuantityCatalogNo.ProBond™ Nickel-Chelating Resin 50 ml150 mlR801-01R801-15Polypropylene columns(empty)50 R640-50Ni-NTA Agarose 10 ml25 ml R901-01 R901-15Ni-NTA Purification System 6 purifications K950-01 Ni-NTA Purification Systemwith Antibodywith Anti-Xpress™ Antibody with Anti-myc-HRP Antibody with Anti-His(C-term)-HRP Antibodywith Anti-V5-HRP Antibody 1 kit1 kit1 kit1 kitK951-01K952-01K953-01K954-01Anti-myc Antibody 50 µl R950-25 Anti-V5 Antibody 50 µl R960-25 Anti-Xpress™ Antibody 50 µl R910-25 Anti-His(C-term) Antibody 50 µl R930-25 InVision™ His-tag In-gel Stain 500 ml LC6030 InVision™ His-tag In-gelStaining Kit1 kit LC6033Pre-Cast Gels and Pre-made Buffers A large variety of pre-cast gels for SDS-PAGE and pre-made buffers for your convenience are available from Invitrogen. For details, visit our web site at or contact Technical Service (page 23).viIntroductionOverviewIntroduction The ProBond™ Purification System is designed for purification of 6xHis-tagged recombinant proteins expressed in bacteria, insect, and mammalian cells. Thesystem is designed around the high affinity and selectivity of ProBond™Nickel-Chelating Resin for recombinant fusion proteins containing six tandemhistidine residues.The ProBond™ Purification System is a complete system that includespurification buffers and resin for purifying proteins under native, denaturing,or hybrid conditions. The resulting proteins are ready for use in many targetapplications.This manual is designed to provide generic protocols that can be adapted foryour particular proteins. The optimal purification parameters will vary witheach protein being purified.ProBond™ Nickel-Chelating Resin ProBond™ Nickel-Chelating Resin is used for purification of recombinant proteins expressed in bacteria, insect, and mammalian cells from any 6xHis-tagged vector. ProBond™ Nickel-Chelating Resin exhibits high affinity and selectivity for 6xHis-tagged recombinant fusion proteins.Proteins can be purified under native, denaturing, or hybrid conditions using the ProBond™ Nickel-Chelating Resin. Proteins bound to the resin are eluted with low pH buffer or by competition with imidazole or histidine. The resulting proteins are ready for use in target applications.Binding Characteristics ProBond™ Nickel-Chelating Resin uses the chelating ligand iminodiacetic acid (IDA) in a highly cross-linked agarose matrix. IDA binds Ni2+ ions by three coordination sites.The protocols provided in this manual are generic, and may not result in 100%pure protein. These protocols should be optimized based on the bindingcharacteristics of your particular proteins.Native VersusDenaturingConditionsThe decision to purify your 6xHis-tagged fusion proteins under native ordenaturing conditions depends on the solubility of the protein and the need toretain biological activity for downstream applications.• Use native conditions if your protein is soluble (in the supernatant afterlysis) and you want to preserve protein activity.• Use denaturing conditions if the protein is insoluble (in the pellet afterlysis) or if your downstream application does not depend on proteinactivity.• Use hybrid protocol if your protein is insoluble but you want to preserveprotein activity. Using this protocol, you prepare the lysate and columnsunder denaturing conditions and then use native buffers during the washand elution steps to refold the protein. Note that this protocol may notrestore activity for all proteins. See page 14.1MethodsPreparing Cell LysatesIntroduction Instructions for preparing lysates from bacteria, insect, and mammalian cellsusing native or denaturing conditions are described below.Materials Needed You will need the following items:• Native Binding Buffer (recipe is on page 8) for preparing lysates undernative conditions• Sonicator• 10 µg/ml RNase and 5 µg/ml DNase I (optional)• Guanidinium Lysis Buffer (supplied with the system) for preparing lysatesunder denaturing conditions• 18-gauge needle• Centrifuge• Sterile, distilled water• SDS-PAGE sample buffer• Lysozyme for preparing bacterial cell lysates• Bestatin or Leupeptin, for preparing mammalian cell lysatesProcessing Higher Amount of Starting Material Instructions for preparing lysates from specific amount of starting material (bacteria, insect, and mammalian cells) and purification with 2 ml resin under native or denaturing conditions are described in this manual.If you wish to purify your protein of interest from higher amounts of starting material, you may need to optimize the lysis protocol and purification conditions (amount of resin used for binding). The optimization depends on the expected yield of your protein and amount of resin to use for purification. Perform a pilot experiment to optimize the purification conditions and then based on the pilot experiment results, scale-up accordingly.Continued on next page2Preparing Bacterial Cell Lysate—Native Conditions Follow the procedure below to prepare bacterial cell lysate under native conditions. Scale up or down as necessary.1. Harvest cells from a 50 ml culture by centrifugation (e.g., 5000 rpm for5 minutes in a Sorvall SS-34 rotor). Resuspend the cells in 8 ml NativeBinding Buffer (recipe on page 8).2. Add 8 mg lysozyme and incubate on ice for 30 minutes.3. Using a sonicator equipped with a microtip, sonicate the solution on iceusing six 10-second bursts at high intensity with a 10-second coolingperiod between each burst.Alternatively, sonicate the solution on ice using two or three 10-secondbursts at medium intensity, then flash freeze the lysate in liquid nitrogen or a methanol dry ice slurry. Quickly thaw the lysate at 37°C andperform two more rapid sonicate-freeze-thaw cycles.4. Optional: If the lysate is very viscous, add RNase A (10 µg/ml) andDNase I (5 µg/ml) and incubate on ice for 10–15 minutes. Alternatively,draw the lysate through a 18-gauge syringe needle several times.5. Centrifuge the lysate at 3,000 ×g for 15 minutes to pellet the cellulardebris. Transfer the supernatant to a fresh tube.Note: Some 6xHis-tagged protein may remain insoluble in the pellet, and can be recovered by preparing a denatured lysate (page 4) followed bythe denaturing purification protocol (page 12). To recover this insolubleprotein while preserving its biological activity, you can prepare thedenatured lysate and then follow the hybrid protocol on page 14. Notethat the hybrid protocol may not restore activity in all cases, and should be tested with your particular protein.6. Remove 5 µl of the lysate for SDS-PAGE analysis. Store the remaininglysate on ice or freeze at -20°C. When ready to use, proceed to theprotocol on page 7.Continued on next page3Preparing Bacterial Cell Lysate—Denaturing Conditions Follow the procedure below to prepare bacterial cell lysate under denaturing conditions:1. Equilibrate the Guanidinium Lysis Buffer, pH 7.8 (supplied with thesystem or see page 19 for recipe) to 37°C.2. Harvest cells from a 50 ml culture by centrifugation (e.g., 5000 rpm for5 minutes in a Sorvall SS-34 rotor).3. Resuspend the cell pellet in 8 ml Guanidinium Lysis Buffer from Step 1.4. Slowly rock the cells for 5–10 minutes at room temperature to ensurethorough cell lysis.5. Sonicate the cell lysate on ice with three 5-second pulses at high intensity.6. Centrifuge the lysate at 3,000 ×g for 15 minutes to pellet the cellulardebris.Transfer the supernatant to a fresh tube.7. Remove 5 µl of the lysate for SDS-PAGE analysis. Store the remaininglysate on ice or at -20°C. When ready to use, proceed to the denaturingprotocol on page 11 or hybrid protocol on page 13.Note: To perform SDS-PAGE with samples in Guanidinium Lysis Buffer, you need to dilute the samples, dialyze the samples, or perform TCAprecipitation prior to SDS-PAGE to prevent the precipitation of SDS.Harvesting Insect Cells For detailed protocols dealing with insect cell expression, consult the manual for your particular system. The following lysate protocols are for baculovirus-infected cells and are intended to be highly generic. They should be optimized for your cell lines.For baculovirus-infected insect cells, when the time point of maximal expression has been determined, large scale protein expression can be carried out. Generally, the large-scale expression is performed in 1 liter flasks seeded with cells at a density of 2 × 106 cells/ml in a total volume of 500 ml and infected with high titer viral stock at an MOI of 10 pfu/cell. At the point of maximal expression, harvest cells in 50 ml aliquots. Pellet the cells by centrifugation and store at -70°C until needed. Proceed to preparing cell lysates using native or denaturing conditions as described on the next page.Continued on next page4Preparing Insect Cell Lysate—Native Condition 1. Prepare 8 ml Native Binding Buffer (recipe on page 8) containingLeupeptin (a protease inhibitor) at a concentration of 0.5 µg/ml.2. After harvesting the cells (previous page), resuspend the cell pellet in8 ml Native Binding Buffer containing 0.5 µg/ml Leupeptin.3. Lyse the cells by two freeze-thaw cycles using a liquid nitrogen or dryice/ethanol bath and a 42°C water bath.4. Shear DNA by passing the preparation through an 18-gauge needle fourtimes.5. Centrifuge the lysate at 3,000 ×g for 15 minutes to pellet the cellulardebris.Transfer the supernatant to a fresh tube.6. Remove 5 µl of the lysate for SDS-PAGE analysis. Store remaining lysateon ice or freeze at -20°C. When ready to use, proceed to the protocol on page 7.Preparing Insect Cell Lysate—Denaturing Condition 1. After harvesting insect cells (previous page), resuspend the cell pellet in8 ml Guanidinium Lysis Buffer (supplied with the system or see page 19for recipe).2. Pass the preparation through an 18-gauge needle four times.3. Centrifuge the lysate at 3,000 ×g for 15 minutes to pellet the cellulardebris. Transfer the supernatant to a fresh tube.4. Remove 5 µl of the lysate for SDS-PAGE analysis. Store remaining lysateon ice or freeze at -20° C. When ready to use, proceed to the denaturingprotocol on page 11 or hybrid protocol on page 13.Note: To perform SDS-PAGE with samples in Guanidinium Lysis Buffer, you need to dilute the samples, dialyze the samples, or perform TCAprecipitation prior to SDS-PAGE to prevent the precipitation of SDS.Continued on next pagePreparing Mammalian Cell Lysate—Native Conditions For detailed protocols dealing with mammalian expression, consult the manual for your particular system. The following protocols are intended to be highly generic, and should be optimized for your cell lines.To produce recombinant protein, you need between 5 x 106and 1 x 107 cells. Seed cells and grow in the appropriate medium until they are 80–90% confluent. Harvest cells by trypsinization. You can freeze the cell pellet in liquid nitrogen and store at -70°C until use.1. Resuspend the cell pellet in 8 ml of Native Binding Buffer (page 8). Theaddition of protease inhibitors such as bestatin and leupeptin may benecessary depending on the cell line and expressed protein.2. Lyse the cells by two freeze-thaw cycles using a liquid nitrogen or dryice/ethanol bath and a 42°C water bath.3. Shear the DNA by passing the preparation through an 18-gauge needlefour times.4. Centrifuge the lysate at 3,000 ×g for 15 minutes to pellet the cellulardebris. Transfer the supernatant to a fresh tube.5. Remove 5 µl of the lysate for SDS-PAGE analysis. Store the remaininglysate on ice or freeze at -20° C. When ready to use, proceed to theprotocol on page 7.Preparing Mammalian Cell Lysates—Denaturing Conditions For detailed protocols dealing with mammalian expression, consult the manual for your particular system. The following protocols are intended to be highly generic, and should be optimized for your cell lines.To produce recombinant protein, you need between 5 x 106and 1 x 107 cells. Seed cells and grow in the appropriate medium until they are 80–90% confluent. Harvest cells by trypsinization. You can freeze the cell pellet in liquid nitrogen and store at -70°C until use.1. Resuspend the cell pellet in 8 ml Guanidinium Lysis Buffer (suppliedwith the system or see page 19 for recipe).2. Shear the DNA by passing the preparation through an 18-gauge needlefour times.3. Centrifuge the lysate at 3,000 ×g for 15 minutes to pellet the cellulardebris. Transfer the supernatant to a fresh tube.4. Remove 5 µl of the lysate for SDS-PAGE analysis. Store the remaininglysate on ice or freeze at -20° C until use. When ready to use, proceed to the denaturing protocol on page 11 or hybrid protocol on page 13.Note: To perform SDS-PAGE with samples in Guanidinium Lysis Buffer, you need to dilute the samples, dialyze the samples, or perform TCAprecipitation prior to SDS-PAGE to prevent the precipitation of SDS.Purification Procedure—Native ConditionsIntroduction In the following procedure, use the prepared Native Binding Buffer, NativeWash Buffer, and Native Elution Buffer, columns, and cell lysate preparedunder native conditions. Be sure to check the pH of your buffers before starting.Buffers for Native Purification All buffers for purification under native conditions are prepared from the5X Native Purification Buffer supplied with the system. Dilute and adjust the pH of the 5X Native Purification Buffer to create 1X Native Purification Buffer (page 8). From this, you can create the following buffers:• Native Binding Buffer• Native Wash Buffer• Native Elution BufferThe recipes described in this section will create sufficient buffers to perform one native purification using one kit-supplied purification column. Scale up accordingly.If you are preparing your own buffers, see page 18 for recipe.Materials Needed You will need the following items:• 5X Native Purification Buffer (supplied with the system or see page 18 forrecipe)• 3 M Imidazole (supplied with the system or see page 18 for recipe)• NaOH• HCl• Sterile distilled water• Prepared ProBond™ columns with native buffers (next page)• Lysate prepared under native conditions (page 2)Imidazole Concentration in Native Buffers Imidazole is included in the Native Wash and Elution Buffers to minimize the binding of untagged, contaminating proteins and increase the purity of the target protein with fewer wash steps. Note that, if your level of contaminating proteins is high, you may add imidazole to the Native Binding Buffer.If your protein does not bind well under these conditions, you can experiment with lowering or eliminating the imidazole in the buffers and increasing the number of wash and elution steps.Continued on next page1X Native Purification Buffer To prepare 100 ml 1X Native Purification Buffer, combine:• 80 ml of sterile distilled water• 20 ml of 5X Native Purification Buffer (supplied with the system or see page 18 for recipe)Mix well and adjust pH to 8.0 with NaOH or HCl.Native Binding Buffer Without ImidazoleUse 30 ml of the 1X Native Purification Buffer (see above for recipe) for use as the Native Binding Buffer (used for column preparation, cell lysis, and binding).With Imidazole (Optional):You can prepare the Native Binding Buffer with imidazole to reduce the binding of contaminating proteins. (Note that some His-tagged proteins may not bind under these conditions.).To prepare 30 ml Native Binding Buffer with 10 mM imidazole, combine: • 30 ml of 1X Native Purification Buffer• 100 µl of 3 M Imidazole, pH 6.0Mix well and adjust pH to 8.0 with NaOH or HCl.Native Wash Buffer To prepare 50 ml Native Wash Buffer with 20 mM imidazole, combine:• 50 ml of 1X Native Purification Buffer• 335 µl of 3 M Imidazole, pH 6.0Mix well and adjust pH to 8.0 with NaOH or HCl.Native Elution Buffer To prepare 15 ml Native Elution Buffer with 250 mM imidazole, combine:• 13.75 ml of 1X Native Purification Buffer• 1.25 ml of 3 M Imidazole, pH 6.0Mix well and adjust pH to 8.0 with NaOH or HCl.Continued on next pageDo not use strong reducing agents such as DTT with ProBond™ columns. DTTreduces the nickel ions in the resin. In addition, do not use strong chelatingagents such as EDTA or EGTA in the loading buffers or wash buffers, as thesewill strip the nickel from the columns.Be sure to check the pH of your buffers before starting.PreparingProBond™ ColumnWhen preparing a column as described below, make sure that the snap-off capat the bottom of the column remains intact. To prepare a column:1. Resuspend the ProBond™ resin in its bottle by inverting and gentlytapping the bottle repeatedly.2. Pipet or pour 2 ml of the resin into a 10-ml Purification Columnsupplied with the kit. Allow the resin to settle completely by gravity(5-10 minutes) or gently pellet it by low-speed centrifugation (1 minuteat 800 ×g). Gently aspirate the supernatant.3. Add 6 ml of sterile, distilled water and resuspend the resin byalternately inverting and gently tapping the column.4. Allow the resin to settle using gravity or centrifugation as described inStep 2, and gently aspirate the supernatant.5. For purification under Native Conditions, add 6 ml Native BindingBuffer (recipe on page 8).6. Resuspend the resin by alternately inverting and gently tapping thecolumn.7. Allow the resin to settle using gravity or centrifugation as described inStep 2, and gently aspirate the supernatant.8. Repeat Steps 5 through 7.Storing PreparedColumnsTo store a column containing resin, add 0.02% azide or 20% ethanol as apreservative and cap or parafilm the column. Store at room temperature.Continued on next pagePurification Under Native Conditions Using the native buffers, columns and cell lysate, follow the procedure below to purify proteins under native conditions:1. Add 8 ml of lysate prepared under native conditions to a preparedPurification Column (page 9).2. Bind for 30–60 minutes using gentle agitation to keep the resinsuspended in the lysate solution.3. Settle the resin by gravity or low speed centrifugation (800 ×g), andcarefully aspirate the supernatant. Save supernatant at 4°C forSDS-PAGE analysis.4. Wash with 8 ml Native Wash Buffer (page 8). Settle the resin by gravityor low speed centrifugation (800 ×g), and carefully aspirate thesupernatant. Save supernatant at 4°C for SDS-PAGE analysis.5. Repeat Step 4 three more times.6. Clamp the column in a vertical position and snap off the cap on thelower end. Elute the protein with 8–12 ml Native Elution Buffer (seepage 2). Collect 1 ml fractions and analyze with SDS-PAGE.Note: Store the eluted fractions at 4°C. If -20°C storage is required, addglycerol to the fractions. For long term storage, add protease inhibitors to the fractions.If you wish to reuse the resin to purify the same recombinant protein, wash the resin with 0.5 M NaOH for 30 minutes and equilibrate the resin in a suitable binding buffer. If you need to recharge the resin, see page 17.Purification Procedure—Denaturing ConditionsIntroduction Instructions to perform purification using denaturing conditions with prepareddenaturing buffers, columns, and cell lysate are described below.Materials Needed You will need the following items:• Denaturing Binding Buffer (supplied with the system or see page 19 forrecipe)• Denaturing Wash Buffer, pH 6.0 (supplied with the system or see page 19 forrecipe) and Denaturing Wash Buffer, pH 5.3 (see recipe below)• Denaturing Elution Buffer (supplied with the system or see page 20 forrecipe)• Prepared ProBond™ columns with Denaturing buffers (see below)• Lysate prepared under denaturing conditions (page 11)Preparing the Denaturing Wash Buffer pH 5.3 Using a 10 ml aliquot of the kit-supplied Denaturing Wash Buffer (pH 6.0), mix well, and adjust the pH to 5.3 using HCl. Use this for the Denaturing Wash Buffer pH 5.3 in Step 5 next page.Be sure to check the pH of your buffers before starting. Note that thedenaturing buffers containing urea will become more basic over time. PreparingProBond™ ColumnWhen preparing a column as described below, make sure that the snap-off capat the bottom of the column remains intact.If you are reusing the ProBond™ resin, see page 17 for recharging protocol.To prepare a column:1. Resuspend the ProBond™ resin in its bottle by inverting and gentlytapping the bottle repeatedly.2. Pipet or pour 2 ml of the resin into a 10-ml Purification Columnsupplied with the kit. Allow the resin to settle completely by gravity(5-10 minutes) or gently pellet it by low-speed centrifugation (1 minuteat 800 ×g). Gently aspirate the supernatant.3. Add 6 ml of sterile, distilled water and resuspend the resin byalternately inverting and gently tapping the column.4. Allow the resin to settle using gravity or centrifugation as described inStep 2, and gently aspirate the supernatant.5. For purification under Denaturing Conditions, add 6 ml of DenaturingBinding Buffer.6. Resuspend the resin by alternately inverting and gently tapping thecolumn.7. Allow the resin to settle using gravity or centrifugation as described inStep 2, and gently aspirate the supernatant. Repeat Steps 5 through 7.Continued on next pagePurification Procedure—Denaturing Conditions, ContinuedPurification Under Denaturing Conditions Using the denaturing buffers, columns, and cell lysate, follow the procedure below to purify proteins under denaturing conditions:1. Add 8 ml lysate prepared under denaturing conditions to a preparedPurification Column (page 11).2. Bind for 15–30 minutes at room temperature using gentle agitation (e.g.,using a rotating wheel) to keep the resin suspended in the lysatesolution. Settle the resin by gravity or low speed centrifugation (800 ×g), and carefully aspirate the supernatant.3. Wash the column with 4 ml Denaturing Binding Buffer supplied with thekit by resuspending the resin and rocking for two minutes. Settle theresin by gravity or low speed centrifugation (800 ×g), and carefullyaspirate the supernatant. Save supernatant at 4°C for SDS-PAGEanalysis. Repeat this step one more time.4. Wash the column with 4 ml Denaturing Wash Buffer, pH 6.0 supplied inthe kit by resuspending the resin and rocking for two minutes. Settle the resin by gravity or low speed centrifugation (800 ×g), and carefullyaspirate the supernatant. Save supernatant at 4°C for SDS-PAGEanalysis. Repeat this step one more time.5. Wash the column with 4 ml Denaturing Wash Buffer pH 5.3 (see recipeon previous page) by resuspending the resin and rocking for 2 minutes.Settle the resin by gravity or low speed centrifugation (800 ×g), andcarefully aspirate the supernatant. Save supernatant at 4°C for SDS-PAGE analysis. Repeat this step once more for a total of two washes with Denaturing Wash Buffer pH 5.3.6. Clamp the column in a vertical position and snap off the cap on thelower end. Elute the protein by adding 5 ml Denaturing Elution Buffersupplied with the kit. Collect 1 ml fractions and monitor the elution bytaking OD280readings of the fractions. Pool the fractions that contain the peak absorbance and dialyze against 10 mM Tris, pH 8.0, 0.1% Triton X-100 overnight at 4°C to remove the urea. Concentrate the dialyzedmaterial by any standard method (i.e., using 10,000 MW cut-off, low-protein binding centrifugal instruments or vacuum concentrationinstruments).If you wish to reuse the resin to purify the same recombinant protein, wash the resin with 0.5 M NaOH for 30 minutes and equilibrate the resin in a suitable binding buffer. If you need to recharge the resin, see page 17.。

给外企公司发邮件介绍自己公司的英语作文全文共6篇示例，供读者参考篇1Dear Foreign Company,Hi there! My name is Timmy and I'm 10 years old. I'm writing to tell you about my dad's really cool company. He said I could write to you since you might want to work with them. Get ready, because this is going to be awesome!My dad started his company 5 years ago in our garage. He's really smart and had a great idea for a new kind of widget that nobody had ever made before. The widget can do all篇2Dear Sir or Madam,My name is Timmy and I am 10 years old. I go to Oakwood Elementary School. I wanted to tell you about my dad's really cool company that he runs!Dad's company is called Evergreen Technologies. It makes software and apps for businesses. My dad says their main thing isenterprise resource planning (ERP) systems. I'm not totally sure what that means, but I know it's about helping companies be more organized and efficient with their resources like money, people, supplies, and time.Dad's company has been around for 15 years now. He started it in our garage back in 2008 with just a few people working for him. But it grew really fast because their products are so good at helping businesses. Now they have over 200 employees!The software they make is used by tons of major companies all over the world. Some of the biggest names that use Dad's ERP software are Amazon, Disney, Ford, and Nestle. Isn't that awesome? The software runs on regular computers but also tablets and smartphones so people can access it anywhere.Their main ERP system is called OmniResource. It has a million different tools and capabilities packed into one platform. It can handle inventory management, supply chain logistics, financial reporting, human resources, customer relations, and so much more. Basically it's like an all-in-one console that puts everything a business needs at your fingertips.But OmniResource is highly customizable too. That means it can be adjusted and tailored perfectly for each client's specificneeds, whatever industry they are in. The developers at Evergreen Technologies work closely with each new client to understand their processes and then configure OmniResource to mesh seamlessly. There are also hundreds of compatibleadd-ons and integrations available.Another really cool product is their business intelligence and analytics dashboard called InsightCore. This mines all the realtime data being captured by OmniResource and puts it into beautiful visualizations like charts, graphs, and scorecards. The dashboards are totally customizable and let you drill down to uncover key metrics and trends. Dad says this kind of advanced analytics is crucial for making smart, data-driven decisions quickly.I think the best part about Dad's company though is their customer support services. With every ERP system implemented, they provide unlimited technical support, training resources, and professional consultants to make sure clients are successful. Their global support team is available 24/7 over multiple channels like phone, email, chat, and online portals.They also offer all kinds of other services too like data migration, system integration, process optimization, change management, and more. Dad says they are really a full-servicesolutions provider committed to their client's long-term growth and profitability, not just selling software.What impresses me most is the company culture at Evergreen Technologies. Dad is always talking about their core values of excellence, innovation, integrity and teamwork. He says those values are embedded into everything they do.Everyone who works there, even the developers, has excellent communication skills and emotional intelligence. They all get tons of training on active listening, clear communication, problem-solving, and understanding the customer's perspective. Dad says that's just as important as technical ability.There's a huge emphasis on creativity and continuously improving their products too. Dad has this thing called the Innovation Incubator, which is a cool space where people can explore new ideas and technologies. Employees can apply to get dedicated time and resources to work on personal projects that could potentially benefit customers.The workplace itself sounds really fun and vibrant from how Dad describes it. There are open collaborative spaces, gaming lounges, fitness centers, and little cafes and coffee shops inside the offices. Employees can even bring their dogs to work! Thereare all sorts of clubs, events, and team outings too like hiking, bowling, hackathons, you name it.Dad says those kinds of perks and creating an engaging culture is crucial for attracting and keeping top talent. He really cares about employee wellbeing, professional development, work-life balance, and fostering a sense of community. That's part of why the company has been so successful.Well, I could probably go on and on, but I don't want this email to get too long and boring! I just think my dad's company is the best and wanted to tell you all about it. Maybe you'd be interested in using their ERP system or services for your business too? Just let me know if you have any other questions!Thanks for reading,Timmy篇3Dear Foreign Company,Hi there! My name is Timmy and I'm 10 years old. I'm writing to tell you all about my dad's company. He asked me to introduce it to you since you might want to work with them. I hope you like learning about it!My dad's company is called Bright Ideas Inc. They make really cool technology products that help make people's lives easier and more fun. Their main office is in a big city near where I live. It's a tall glass building right downtown that has their logo on the very top. Whenever we drive by, I always get excited to see it!Inside the office, it's really modern and colorful. The floors are bright blue and there are red chairs and couches everywhere for people to sit and have meetings. My dad says the fun colors help people be more creative when they're working. There are also lots of big windows that let in natural sunlight. My favorite part is the cafeteria because they have a soft-serve ice cream machine that gives free ice cream to all the employees!The people who work at Bright Ideas are seriously the coolest. Instead of boring suits, they wear jeans and t-shirts to the office. Some of them even have colored hair or tattoos! My dad says they hire people from all over the world with different backgrounds to get diverse perspectives. He once took me for Show and Tell day and I got to meet engineers from India, designers from France, and marketers from Brazil. I was amazed at how many languages I heard being spoken in the hallways.But the best part about the employees is how passionate they are about their work. Whenever I ask my dad about his day, he gets so excited telling me about the new products they're developing and the challenges they're trying to solve through technology. He says everyone at Bright Ideas loves pushing the boundaries and thinking outside the box. No idea is too crazy or far-fetched for them to at least consider! That's how they come up with such innovative and groundbreaking products.Speaking of products, you probably want to know what Bright Ideas actually makes, huh? Well, they've created some really awesome gadgets and gizmos that make life more efficient and entertaining. One of their biggest hits is the SmartCube, which is like a super advanced smart speaker. Not only can you use it to play music, check the weather, and do internet searches like other speakers, but the SmartCube can actually learn your daily routine and habits over time. It can give you customized advice and suggestions to optimize your schedule, remind you of appointments, and even control other smart home devices you might have like lights or the thermostat. My dad got me one for my bedroom and it's been a total game-changer for keeping me organized with homework and activities!Another popular Bright Ideas invention is the JoyScroll, a digital screen that displays constantly changing images, videos, artwork, quotes, and animations. Unlike a regular picture frame, the images on the JoyScroll never repeat and are sourced from a massive library of content as well as user submissions. It's designed to spark happiness, curiosity, and imagination as you watch it cycle through different visuals. My parents have one hanging in our living room and I'm always excited to see what creative new scenes will appear on it.But my personal favorite Bright Ideas product is definitely the AuraVisor. It's a headset that uses augmented reality to project educational games and experiences into the world around you. Want to visit ancient Rome and watch gladiators fight? The AuraVisor can make it look like you're sitting in the Colosseum. Or if you want to see what the solar system looks like, it can put realistic 3D planet models right in your bedroom for you to explore and learn about. With the AuraVisor, boring school subjects come alive in such a fun and immersive way. The graphics and animations are super realistic too - sometimes I forget I'm not actually shrunk down in a virtual world! My best friend got one for his birthday and we could seriously spend hours adventuring through the different experiences.Those are just a few examples of the amazing products Bright Ideas creates, but they're always working on new ones. From what I've overheard my dad mention, some top secret projects they have in development right now include holographic video displays, self-navigating delivery drones, and even a virtual habitat for endangered animal species! He says we'll need to exercise a lot of patience though because their engineers are real perfectionists when it comes to quality.In the meantime, Bright Ideas makes plenty of money from all their existing inventions being sold worldwide. That lets them reinvest tons of money into research, development, and hiring more employees to keep pushing technological boundaries. My dad tells me that despite the company's huge success, their mission stays the same - using creativity and innovation to develop products that better people's lives in meaningful ways while also sparking wonder and delight.So in summary, Bright Ideas Inc is an amazing, cutting-edge technology company filled with brilliant, passionate employees of all backgrounds. They prioritize fun and creativity to churn out one game-changing, smile-inducing product after another. Partnering with them would not only be an incredible opportunity for your company, but by supporting their mission,you'd be making the world a little bit better (and cooler!) of a place.I really hope you decide to work with Bright Ideas because it would be so awesome to have my dad's company collaborating with yours. Just imagine what amazing innovations you could create and experiences you could bring into this world by joining forces! Maybe I'll even get to be a beta tester for your first joint product launch. How cool would that be?!Anyway, thanks so much for taking the time to learn about Bright Ideas. I tried my best to tell you everything I could about them. If you have any other questions, I'd be happy to ask my dad or give you a tour of the office myself! Please let me know if you're interested in partnering up. I'll keep my fingers crossed!Sincerely,Timmy篇4Dear Foreign Company,Hi there! My name is Tommy and I'm 10 years old. I'm writing to tell you about this really cool company that my dadworks for. It's called Acme Widgets Inc. and they make the best widgets in the whole world!Acme Widgets has been around for a super long time, like 75 years! That's older than my grandparents. It was started way back in 1948 by old Mr. Acme himself. He came over from Italy after World War 2 ended and didn't have much money. But Mr. Acme was really good at making widgets, so he started a tiny little workshop in his garage to make them.At first, it was just Mr. Acme making widgets all by himself. He worked really hard every single day. Pretty soon, people started noticing how great his widgets were. They were strong and lasted forever! So lots of people wanted to buy Mr. Acme's widgets. His little garage business got too small, and he had to hire some other people to help make enough widgets for everyone.As the years went by, more and more people wanted Acme's amazing widgets. The company kept growing bigger and bigger. They moved to a huge factory and now they have over 5,000 employees! Can you imagine having a job where 5,000 other people work too? That's like a whole town!My dad has worked at Acme Widgets for 15 years. He's one of the engineering managers, which means he's kind of like theboss of the engineers. The engineers are the people who design all the different widget styles and make sure they work perfectly. Dad says it's a really hard job because all the engineers always have to come up with new ideas to make the widgets better and better.You wouldn't believe all the cool machines they use to make widgets at Acme's factory. There's this one huge machine that's taller than a monster truck! It takes raw metal and smashes it and melts it and shapes it into widget blanks. The blanks go through all these other crazy machines that drill holes, stamp on labels, add the fiddly little components, and more. I went on a tour of the factory once and it was so loud with all the machines clanking and buzzing. But it was awesome!After the widgets are all assembled, they have to get painted. My favorite part of the factory is the painting room. It's this big room with robots that take the plain metal widgets on a conveyor belt and spray them with super cool colors - red, blue, orange, purple, any color you can imagine! The robots move the widgets around so they get painted evenly without any missed spots. It's mesmerizing to watch.Then the painted widgets go through a big oven to make the paint dry and hardened. Once they cool off, they get packagedup and are ready to ship to customers all over the world. Acme has customers in over 80 countries! The truckers come and load up the huge pallets of boxed-up widgets to take them to ships, trains, and airplanes to send them everywhere.Acme Widgets has won tons of awards for having high quality products. In 2015, they won the Global Manufacturing Excellence award. Then in 2018, they won the Governor's Award for being an outstanding company in our state. Just last year in 2023, the National Safety Council gave Acme their highest honor for having a really safe workplace and no accidents.I think the number one reason Acme Widgets is so successful is because they genuinely care about making a good product that works well and lasts a very long time. Dad says they could probably make cheaper, flimsier widgets if they wanted to cut corners. But they choose to use premium materials and have rigid quality control instead. That's because the owners and managers want customers to be 100% satisfied.Another key thing is that Acme really values their employees and treats them extremely well. The workers get decent pay, full benefits, lots of paid vacation days, and big bonuses if the company profit goals are met. My dad says the bosses make an effort to listen to employees' ideas and to make the factory asafe, comfortable place to work. Happy workers means better quality!So that's the scoop on Acme Widgets Inc. in a nutshell! They've been around forever and are on the top of their game when it comes to making outstanding widget products. Maybe your company could be one of their many satisfied customers too? Just a thought!Anyway, I hope you found this introduction helpful and not too boring. Let me know if you have any other questions! I'll do my best to answer or get my dad to explain more about the company he works for. Thanks for reading my letter!Your friend,Tommy篇5Here's an email written in English to introduce your company to a foreign company, from the perspective of a primary school student, with a length of around 2000 words:Subject: Hello from Little Star Company!Hi there!My name is Lucy, and I'm a student in 5th grade. I'm writing to tell you all about my family's company called Little Star Company. We're a small business, but we have big dreams and lots of fun things to share with you!First, let me tell you how Little Star Company got started. A few years ago, my mom and dad were both working at big companies, but they didn't feel very happy there. They wanted to do something they really loved and could be proud of. That's when they had the idea to start their own company!My parents are super creative people. Mom is an artist who loves painting and making jewelry, while Dad is a whiz at woodworking and building cool stuff out of recycled materials. They decided to combine their talents and make beautiful,eco-friendly products that people would enjoy.Little Star Company is all about sustainability and protecting the environment. We use recycled materials as much as possible, like old wood, glass, and even scraps of fabric. It's amazing what you can create out of things that would otherwise end up in the trash!Our most popular products are handmade wooden toys, like puzzles, building blocks, and cute little animal figurines. Dad designs and carves each one with so much care and attention todetail. They're not only fun to play with but also nice to look at and display.Mom's specialty is jewelry made from recycled glass, beads, and metal. She makes the prettiest necklaces, earrings, and bracelets in all sorts of colors and styles. Some have little charms or gemstones, and others are simple but elegant. People love wearing her creations because they're unique and environmentally friendly.We also sell home decor items like vases, candle holders, and picture frames, all made from recycled materials. Mom and Dad put a lot of thought into the designs, making sure each piece is both beautiful and practical.One of the coolest things about Little Star Company is our commitment to giving back. For every product we sell, we donate a portion of the profits to organizations that protect forests, oceans, and endangered species. We want to do our part to make the world a better place for all living creatures, big and small.Running a small business is a lot of work, but my parents love it. They get to be creative every day and share their passion with others. Sometimes I help out by designing labels, packaging orders, or coming up with new product ideas (like a line ofsuperhero action figures made from old bottles – how cool would that be?!).I'm so proud of my mom and dad for following their dreams and starting Little Star Company. Even though we're a tiny business, we have a huge mission: to spread joy, beauty, and eco-friendliness wherever we go!I hope you've enjoyed learning about our company. If you're ever in need of unique, handmade gifts or home decor, be sure to check us out. We'd love to collaborate with your company and share our products with people all around the world.Thank you for taking the time to read my letter. I'll leave you with a fun fact: did you know that stars are actually giant balls of hot gas? Pretty cool, right? I'll have to tell you more star facts next time!Your friend,LucyLittle Star Company篇6Dear Foreign Company,Hi there! My name is Tommy and I'm 10 years old. I love playing video games, reading comic books, and hanging out with my friends. But today I'm going to tell you all about my dad's company. It's pretty cool!My dad started his company 5 years ago in our city. He used to work for a big corporation, but he didn't like how they treated their employees and customers. So he decided to start his own business doing things his way - the right way!The name of the company is Sunshine Solutions. I helped my dad pick out the name because sunshine makes me happy, and my dad wants his company's solutions to make people happy too! Isn't that a nice idea?Sunshine Solutions provides cloud computing and data storage services to other businesses. I'm not totally sure what that means, but from what I understand, they help companies use the internet to do their work and store their important files safely. Pretty neat, huh?My dad says that a lot of companies these days need reliable cloud services to operate efficiently. Sunshine Solutions offers cloud platforms that are secure, scalable, and customizable to meet each client's unique needs. Their data centers usecutting-edge technology to protect information and ensure 24/7 uptime. Grown-ups sure do love their tech jargon!What makes Sunshine Solutions special is their commitment to awesome customer service. My dad always says to "treat others how you want to be treated," and that's exactly how they operate. They have a team of friendly experts who provide training, support, and technical guidance whenever clients need it.Every year, Sunshine Solutions invests a portion of their profits into developing new cloud solutions and enhancing their infrastructure. My dad wants to stay ahead of the curve and keep getting better at what they do. He's always reading tech blogs and going to conferences to learn about the latest trends. Sometimes it's hard to get his attention when he's in "work mode!"Another really cool thing about my dad's company is how much they value their employees. Sunshine Solutions offers competitive pay, great benefits, and a fun work environment. They have casual dress code, game rooms to take breaks, and even bring your dog to work days! My dad says happy employees do better work. He's a wise guy, that's for sure.Every few months, the whole company gets together for a special event called "Sunshine Day." We all go to a park or recreation center and spend the day hanging out, eating tasty food, and playing games. Last year, we had a huge waterballoon fight! My dad got totally soaked but he didn't even mind. He was just happy we were all having fun together. Those Sunshine Days are some of my favorite memories.I probably shouldn't say this, but my dad tells me that a lot of their competitors charge way too much and don't care about customer happiness. At Sunshine Solutions, they try to keep their prices reasonable so small businesses can afford their services. And if a client ever has an issue, my dad's team will work hard to fix it right away. No customer gets left behind!Oh, I almost forgot to mention their new "green" initiatives! Sunshine Solutions recently started using renewable energy sources like solar and wind to power their operations. They want to reduce their carbon footprint and be environmentally-friendly. We even have a company garden where we grow our own vegetables! Getting our hands dirty is fun and it's healthy too.So in summary, Sunshine Solutions offers top-notch cloud computing services, prioritizes customer satisfaction, values their employees, and is committed to sustainable practices. And at theheart of it all is my dad - a hard-working, ethical businessman who really cares about making the world a little bit better.I'll be honest, running a company seems like a lot of work to me. But my dad seems to get a lot of joy and pride from it. He puts in long hours, but he's always happy when he comes home.I guess that's the great thing about doing work you're passionate about and believe in.Anyway, that's the scoop on Sunshine Solutions! I may only be 10 years old, but I can already tell it's a pretty special company. If you ever need some cloud computing solutions, you should definitely give them a chance. Just don't be surprised if you get a friendly welcome message from me!Thanks for reading my little essay. I hope you have an awakwardly wonderful day!Sincerely,Tommy。

OPERATOR’S MANUALBD ProbeTec™ ET Lysing HeaterBD ProbeTec™ ET Priming and Warming HeaterBecton, Dickinson and Company7 Loveton CircleSparks, Maryland 21152 USA800-638-8663BENEX LimitedBay K 1a/d, Shannon Industrial EstateShannon, County Clare, IrelandTel: 353-61-47-29-20Fax: 353-61-47-25-462003/05UNPACKING INSTRUCTIONSExamine the carton, blocks and heater(s) for damage. If damage is apparent, contact your Becton Dickinson representative. Retain all packaging material in the event that the unit must be returned.1. Remove the top layer of packing material from the shipping box.2. Unpack and verify the contents of your package.a. The Lysing Heater carton should contain: Lysing Heater with heaterblock, power cord, thermometer (temperature range 95-120°C),Lyse Cover, and Operator’s Manual.b. The Priming and Warming Heater carton should contain: Primingand Warming Heater, power cord, (2) thermometers (temperaturerange 50 -75°C), thermally protective glove, and Operator’sManual.SUMMARY OF WARNINGS AND CAUTIONS:WARNING! The protection provided by this product may be impaired if the equipment is used in a manner not consistent with the instructions in this manual.temperatures in excess of 100°C. Extreme caution should beexercised when the unit’s power is on and until the temperaturereaches 50°C after the unit is turned off. A lyse cover isprovided to cover the heater when not in use.WARNING! Burn Hazard! The Priming heater block component of the Priming and Warming Heater reaches temperatures in excess of 70°C. Extreme caution should be exercised when the unit’s power is on and until the temperature reaches 50°C after the unit is turned off.WARNING! The Heatblock units are designed for operation in dry conditions. DO NOT pour water, oil, or other fluids into the unit. This is a safety hazard. The chamber that the block sits in is not designed to be filled with liquid or other fluids. DO NOT place anything other than the module block in this cavity. WARNING! Wipe up spills immediately to prevent electrical shock. Biohazard spills should be cleaned up using approved laboratory procedures. Solvent spills are a fire hazard. Turn power off immediately and do not operate until clean up is complete and vapors have dissipated.WARNING! Do not operate the unit if it shows signs of electrical or mechanical damage.WARNING! To avoid danger of fire and/or explosion, do not heat fluids or compounds beyond their auto-ignition temperatures.Caution! Unit assumes previous setpoint when power is reapplied.Caution! Seal Amplification Microwells before Transferring Plate to BDProbeTec ET InstrumentSUMMARY OF POWER SPECIFICATIONS:HEATER MODEL POWER REQUIRED +/-10% WATTS Lysing Heater 440483 100-120V/50-60Hz 500 Lysing Heater 440482 220-240V/50 Hz 500 Priming andWarming Heater 440480 100-120V/50-60Hz 500 Priming andWarming Heater 440479 220-240V/50 Hz 500 POWER FUSES: 5mm X 20MM, 5 Amp quick actingEnvironmental Requirements:StorageNon-OperatingTemperature:-20°to 65°C (-4°F to 149°F)Humidity: 20% - 85% RH, non-condensingConditions:OperatingOnlyUseIndoorTemperature: 18° to 33°C (64.4° F to 91.4°F)Humidity:non-condensing85%RH,20%toAltitude: 0 to 6,562 ft. (2,000 M) above sea levelInstallation Category II and Pollution Degree 2 in accordancewith IEC 664.INITIAL SET-UP1. Place the heater in an area free of air currents. Air currents can affecttemperature stability. Ensure that the surface on which the unit isplaced will withstand typical heat produced by the unit.2. The 120 volt units are supplied with a three prong line cord that shouldbe plugged into a standard three prong grounded outlet (in the USA).The 220 volt units are supplied with 2 line cords to meet mostcountries’ requirements. Ensure the power outlet is properly grounded.3. Insert the thermometer(s) into the thermometer well(s).NOTE: Do not interchange the Lysing Heater thermometer with the Priming and Warming Heater thermometers. The two thermometer types have different temperature scales. The Lysing Heater thermometer ranges 95-120°C. The two thermometers for the Priming and Warming Heater range between 50-75°C.INSTRUMENT CONTROLSEach heater has it’s own LED readout. The display alternates between the actual and setpoint temperatures. The three controls are defined below: ACTUAL TEMPERATURE INDICATORThe Actual temperature indicator is located at the front of the unit, at top left. It is marked with a “thermometer” icon. The indicatorilluminates green when the actual temperature is displayed on the LEDreadout.SETPOINT TEMPERATURE INDICATORThe Setpoint temperature indicator is located at the front of the unit, at the top right. It is marked with “thermometer” and “target” icons.When the setpoint temperature is displayed on the LED readout, theindicator illuminates green.UP AND DOWN ARROW KEYSThe UP and DOWN Arrow Keys are used to increase (UP arrow) or decrease (DOWN arrow) the Setpoint temperature. Press and release a key to change the temperature in the desired direction by 0.1°C. If the key is pressed and held, the temperature changes rapidly. Release the keywhen the desired temperature is reached. NOTE: The Setpoint isadjusted at the factory. You typically will not need to adjust thesetpoint.CONTROLCALIBRATIONThe Calibration Control is located at the bottom left on the front of the unit. This control allows you to adjust the displayed temperature toagree with the readings of a calibrated laboratory thermometer. See theCalibration section for instructions.OPERATION1. Turn the power switch on the back of the heater to “ON” or “I”. Thedisplay alternates between the actual temperature and the setpointtemperature.2. To set the desired temperature, use the up arrow and down arrowkeys. Holding either key down will cause the setpoint temperature tochange rapidly. A single pressing of either key will move the setpointtemperature by 0.1°C. There are no “set” or “enter” keys to press.Once the setpoint temperature is programmed, it is accepted. Whilethe keys are depressed, the display shows the setpoint. Once thesetpoint is programmed and the keys are not depressed, the displayalternates between setpoint and actual temperatures. (See assayspecific package inserts for appropriate set temperatures.)3. Allow time for the temperature to stabilize (approximately 90 minutes).HEATER CALIBRATION PROCEDURE(NOTE: The heater temperature must be stabilized prior to starting the calibration procedure. To ensure stability, wait 90 minutes after instrument is turned ON. Wait 30 minutes after an incubation.)1. Enter the Calibration mode by pressing the up and down arrow keyssimultaneously. The word “CAL” is displayed for approximately 5 seconds and continues to flash the word “CAL” every 5 seconds. The temperature is nowfixed in Actual Temperature mode and the small green indicators no longer flash from Setpoint to Actual temperature.2. Use the small flathead screwdriver (included in BDProbeTec ET SystemStart-up Kit) to turn the calibration control screw to adjust the temperature readout to match the measured (thermometer) temperature.3. Exit the calibration mode by pressing both arrow keys simultaneously. Theword “CAL” will be displayed for approximately 5 seconds. The readout will then alternately display Setpoint and Actual temperatures in correspondence with the green indicators.4. Allow 60 minutes for temperature equilibration.5. After equilibration, verify that the thermometer reads as follows:Lysing Heater: within +/- 2.0°C of the Setpoint TemperaturePriming and Warming Heater: within +/- 0.5°C of the Setpoint Temperature CLEANING PROCEDUREWARNING! DO NOT pour water, oil, or other fluids into the unit. ReviewSUMMARY OF WARNINGS section.Wipe each unit with paper towels wetted with 1% (v/v) sodium hypochlorite solution. Re-wipe the unit with paper towels dampened with distilled or deionized water. Dry the unit by wiping with clean dry paper towels.MAINTENANCE AND SERVICINGDisconnect the power to the unit before servicing. Your Heaters are built for long, trouble free, dependable service.NOTE: No user serviceable components are inside the unit.FAILURE INDICATIONSIf the unit display flashes “SSSS” it may mean there is a faulty temperature sensor or a break in the wiring from the sensor. If the unit display flashes “HHHH” it is exceeding the maximum allowable temperature. In either case, switch the unit off and contact your Becton Dickinson representative for repairs.For Service in North America Call:Becton, Dickinson and CompanyTechnical Service: 1-800-638-8663Field Service: 1-800-544-7434For Complete List of International Contactssee your BD ProbeTec™ ET System User’s ManualBD ProbeTec™ ET is a Registered Trademark of Becton, Dickinson and Company553032-00。

高科技公司介绍英语作文1. This high-tech company is a game-changer in the industry. They are constantly pushing the boundaries of innovation and revolutionizing the way we live and work. Their cutting-edge technology and groundbreaking solutions have transformed various sectors, from healthcare to transportation.2. The company's products are known for their sleek design and user-friendly interface. They prioritize user experience and strive to create intuitive and seamless interactions. Their devices and software are not only functional but also aesthetically pleasing, making them highly sought after by consumers around the world.3. One of the key strengths of this high-tech company is their commitment to research and development. They invest heavily in exploring new technologies and discovering novel solutions to complex problems. Their team of brilliant scientists and engineers work tirelessly tostay ahead of the curve and anticipate future trends.4. In addition to their technological prowess, this company also places great emphasis on sustainability and environmental responsibility. They actively seek ways to minimize their carbon footprint and develop eco-friendly products. From energy-efficient devices to recyclable packaging, they are dedicated to making a positive impact on the planet.5. Another notable aspect of this high-tech company is their strong focus on collaboration and diversity. They believe that innovation thrives in an environment where different perspectives and ideas are welcomed. They foster a culture of inclusivity and actively seek out talent from diverse backgrounds, ensuring a rich and dynamic workforce.6. This company is not only a leader in the industry but also a responsible corporate citizen. They actively engage in philanthropic initiatives and give back to the communities they operate in. From supporting education programs to funding environmental conservation projects,they strive to make a meaningful difference in society.7. The success of this high-tech company can be attributed to their unwavering commitment to excellence. They set high standards for themselves and consistently deliver products and services of exceptional quality. Their dedication to customer satisfaction and their ability to adapt to changing market demands have earned them a loyal customer base.8. Looking to the future, this high-tech company shows no signs of slowing down. They continue to invest in research and development, exploring emerging technologies such as artificial intelligence and virtual reality. With their innovative spirit and forward-thinking mindset, they are poised to shape the future of technology and continue to be at the forefront of the industry.。

写一篇关于招聘广告的英语作文全文共3篇示例，供读者参考篇1Writing an Essay about Job AdvertisementsJob advertisements are a fact of life for anyone seeking employment. As a student looking to enter the workforce after graduation, I've spent a lot of time analyzing and dissecting these little Windows into potential careers. Job ads provide crucial information that can make or break your decision to apply for a role. At their core, they aim to attract qualified candidates by selling the position and company. However, there's often an art to reading between the lines to gauge if it's truly a good fit.A well-crafted job ad will immediately grab your attention with a compelling job title. This functions as a sort of headline to pique your interest. Vague titles like "Exceptional Opportunity" are red flags that reek of desperation, while overtly specific ones like "Social Media Marketing Coordinator for Eco-Friendly Pet Product Company" give you a clear picture of what to expect. The best titles strike a balance by being concise yet informative.After the title hooks you, the ad should succinctly describe the core responsibilities and key qualifications required. This serves as a quick litmus test to see if you're a potential fit before diving deeper. I can't count how many ads I've ignored after reading I'd need five years of experience in a niche field for an entry-level job. Making it past this section means you're still a contender.The meat of the ad is the detailed job description and requirements. This is where companies attempt to paint their opportunity in the most attractive light possible. They'll lure you in with perks like a competitive salary, full benefits, growth opportunities, and a dynamic work culture. Be cautious of any ad that's too vague on the actual job duties though. There's a difference between empowering self-starters and having no idea what the role entails.Smart job seekers read between the lines to decipher any potential red flags. For example,edig into the core responsibilities and qualifications listed. Do they seem reasonable for the role and your experience level? Or are they asking for martyr-level commitments like being available 24/7 for an average salary? If you see unrealistic expectations like requiring a doctorate for an entry-level analyst position, that's aclear sign the employer is out of touch or has questionable management practices.Analyzing the company overview and culture sections is equally revealing. These are where employers humble brag about their company's accomplishments and work environment. Alarm bells should ring if they rely too heavily on corporate jargon or make it sound like a cult. "Fast-paced," "driven," and "entrepreneurial" can translate to overworked, unrealistic expectations, and chaos. Conversely, phrases like "kick back" or "limited supervision" might mean you'll be thrown in and left to fend for yourself.The benefits and compensation details deserve careful examination too. If an ad omits this information completely, assume the worst - they're likely subpar or they'd be shouting it from the rooftops to lure top talent. That said, benefits and perks shouldn't make up for other shortcomings if the role itself seems like a bad fit. I've definitely been tempted by ads boasting unlimited vacation, free lunches, and ping pong tables, only to realize the actual job sounded miserable upon closer inspection.Smart employers invest time to craft ads that attract their ideal candidates. Shouldn't you invest similar diligence as a job seeker to analyze if an opportunity deserves your time andapplication? The ultimate litmus test is whether the ad resonates with you and inspires genuine interest and enthusiasm for the role. Countless free resources can shed light on an employer's reputation too. If red flags keep surfacing, no amount of polished language should persuade you to settle.In today's competitive job market, finding a mutually rewarding fit is critical for both employers and candidates. Job ads offer the first glimpse into an opportunity's potential - if read critically. As I prepare to take my next career step, I've become adept at separating facts from fluff and hype. A well-written, thoughtful ad is often the first sign of a quality employer worth pursuing.篇2Writing an Effective Job AdvertisementAs a student, I recently had the opportunity to work on a project that involved crafting a job advertisement for a hypothetical company. This task taught me valuable lessons about the art of attracting top talent through well-written job postings. In this essay, I will share my insights and experiences, highlighting the key elements that make a job ad compelling and effective.The Importance of a Clear and Concise Job TitleThe job title is the first thing that catches a potential applicant's eye, so it's crucial to make it clear and concise. A vague or overly complicated title can deter qualified candidates from even considering the position. For our project, we aimed to create a title that accurately reflected the role's responsibilities while also being easily searchable and memorable.Example: "Digital Marketing Specialist"Crafting an Attention-Grabbing IntroductionThe introduction of a job ad is like the hook of a novel – it needs to capture the reader's interest and make them want to learn more. We focused on crafting an engaging opening paragraph that highlighted the company's mission, culture, and the exciting opportunities the role offered. By painting a compelling picture, we aimed to pique the interest of potential candidates and encourage them to continue reading.Example: "Are you a skilled digital marketer with a passion for driving innovative campaigns? Join our dynamic team at [Company Name], where we're revolutionizing the [industry] with cutting-edge solutions. In this role, you'll have theopportunity to shape our digital presence, craft compelling content, and drive brand awareness across multiple platforms."Outlining Key Responsibilities and QualificationsThe meat of a job advertisement lies in the detailed description of the role's responsibilities and the qualifications required. We spent a significant amount of time carefully crafting these sections to ensure they were comprehensive yet concise. By breaking down the duties into clear bullet points and listing the essential skills, educational background, and experience needed, we aimed to provide potential applicants with a transparent understanding of what the role entailed.Example:Key Responsibilities:Develop and implement comprehensive digital marketing strategies across various channels (social media, email, paid advertising, etc.)Collaborate with cross-functional teams to create engaging content that aligns with brand messagingAnalyze campaign performance data and optimize efforts for maximum ROIStay up-to-date with the latest digital marketing trends and best practicesQualifications:Bachelor's degree in Marketing, Communications, or a related field3+ years of experience in digital marketing, preferably in a B2C environmentProficiency in digital analytics tools (e.g., Google Analytics, Hootsuite, etc.)Excellent written and verbal communication skillsStrong analytical and problem-solving abilitiesCreativity and a passion for staying ahead of the curveHighlighting Company Culture and BenefitsBeyond the job details, we recognized the importance of showcasing the company's culture and the benefits it offers to employees. This section can be a powerful differentiator, as top talent often seeks organizations that align with their values and provide a positive work environment. We aimed to paint an attractive picture of the company's culture, emphasizing aspectssuch as work-life balance, professional development opportunities, and unique perks.Example: "At [Company Name], we foster a collaborative and innovative culture where teamwork and creativity thrive. Our employees enjoy a dynamic work environment, comprehensive benefits (including health insurance, 401(k) matching, and generous paid time off), and opportunities for professional growth through training and mentorship programs. We also prioritize work-life balance, offering flexible schedules and remote work options."Closing with a Call to ActionFinally, we concluded the job ad with a strong call to action, encouraging qualified candidates to apply. We provided clear instructions on how to submit their applications, including any required materials (such as a resume, cover letter, or portfolio). By making the application process straightforward and accessible, we aimed to remove any potential barriers that could deter talented individuals from pursuing the opportunity.Example: "If you're a driven digital marketer with a passion for innovation and a track record of success, we want to hear from you! Submit your resume, cover letter, and a brief portfolio showcasing your previous work to [email/application portal] by[deadline]. Together, we can take [Company Name] to new heights and shape the future of [industry]."Crafting an effective job advertisement is both an art and a science. It requires a deep understanding of the role, the target audience, and the company's unique selling points. Through this project, I gained invaluable experience in creating compelling job postings that not only attract qualified candidates but also effectively communicate the company's culture, values, and opportunities for growth.As I continue my academic and professional journey, I will carry these lessons with me, recognizing the power ofwell-crafted job advertisements in attracting top talent and shaping the future success of organizations.篇3Title: Crafting an Effective Job AdvertisementThe world of employment is ever-evolving, and companies are constantly seeking talented individuals to fill vacant positions. In today's competitive job market, crafting an effective job advertisement is crucial in attracting the right candidates. As a student pursuing a degree in marketing, I have gained valuableinsights into the art of creating compelling job ads that resonate with potential applicants.IntroductionA well-crafted job advertisement serves as a powerful tool for companies to showcase their brand, attract top talent, and ultimately fill open positions with the best-fit candidates. It is the first point of contact between an organization and prospective employees, making it essential to capture their attention and convey the right message. In this essay, I will delve into the key elements of an effective job advertisement, drawing upon my knowledge and experiences as a marketing student.The Hook: Grabbing Attention from the StartThe opening lines of a job advertisement are akin to the hook in a captivating story – they must immediately capture the reader's interest and entice them to continue reading. An attention-grabbing headline or a concise yet compelling job title is crucial in achieving this goal. Additionally, highlighting the company's unique selling points, such as its reputation, work culture, or growth opportunities, can pique the interest of potential candidates right from the outset.Painting a Clear Picture: Job Description and ResponsibilitiesOnce the reader's attention has been captured, it is essential to provide a clear and comprehensive overview of the job's responsibilities and duties. This section should bewell-structured and easy to comprehend, allowing candidates to quickly determine if the role aligns with their skills, interests, and career aspirations. Using concise and precise language, along with bullet points or numbered lists, can enhance readability and ensure that key information is conveyed effectively.Outlining the Ideal Candidate: Qualifications and RequirementsAfter detailing the job's responsibilities, the next step is to outline the qualifications and requirements that the ideal candidate should possess. This section should strike a balance between being specific enough to attract qualified applicants while avoiding overly restrictive criteria that may discourage potential talent. It is essential to distinguish between mandatory qualifications and preferred skills or experiences, allowing for some flexibility in the hiring process.Highlighting the Perks: Benefits and IncentivesIn today's competitive job market, offering attractive benefits and incentives can be a powerful differentiator for companies seeking top talent. This section of the jobadvertisement should highlight the company's unique offerings, such as competitive salaries, health insurance, retirement plans, professional development opportunities, or flexible work arrangements. By showcasing these perks, companies can position themselves as desirable employers and attract candidates who align with their values and priorities.Showcasing the Company Culture: Branding and Employer ReputationA job advertisement is not only about filling a vacant position; it is also an opportunity for companies to showcase their brand and culture. By incorporating elements that reflect the organization's values, mission, and work environment, companies can attract candidates who resonate with their culture and are more likely to thrive within the organization. This can include highlighting company achievements, community involvement, or employee testimonials, painting a vivid picture of what it's like to work for the organization.Call to Action: Encouraging Applicants to ApplyFinally, a compelling job advertisement should conclude with a clear and actionable call to action, encouraging qualified candidates to apply. This section should provide straightforward instructions on how to submit applications, including anyrequired documents, submission deadlines, and contact information. Additionally, it is advisable to include a brief statement expressing enthusiasm for receiving applications and highlighting the organization's commitment to diversity and equal opportunity.In conclusion, crafting an effective job advertisement is an art form that requires careful consideration of various elements. By combining attention-grabbing headlines, clear job descriptions, well-defined qualifications, attractive benefits, and a compelling portrayal of the company culture, organizations can increase their chances of attracting the right talent. As a marketing student, I have gained valuable insights into the strategies and techniques involved in creating impactful job advertisements, equipping me with the knowledge to contribute to this crucial aspect of talent acquisition in the future.。

P52-P55:导读：第10届中国国际展览和会议展示会于2009年1月在南京国际展览中心举行，展会主题为：对话、交流、和谐、进步。

The 10th China international Trade Show for Exhibition and Conference IndustryDate :January 14th -16th,2009Place；Nanjing International Exhibition CenterSponsors:China Council for the Promotion of International Trade （CCPIT）The GIobaI Association of the Exhibition Industry (UFI)International Association of the Exhibition and Events (IAEE)Society of Independent Show Organizers (SISO)Organizer:China Association for the Exhibition CentersChina International Exhibition Center Group CorporationCo-organizers:Nanjing Conference Exhibition OfficeNanjing International Exhibition CenterCAEC Professional Committee for Exhibition OrganizationCAEC Professional Committee for Exhibition EngineeringCAEC Professional Committee for Exhibition Theory ResearchSupported by :Jiangsu Easthingh Convention & Exhibition Group Co, LtdJiangsu Provincial Convention & Exhibition Industry AssociationTheme : Dialogue·Communication·Harmony·DevelopmentExhibition Aim:Nanjing , a historic and civilized city, is an important industrial production base. It is an important center of transportation and communication in the eastern region of China, and a national base of superior education and scientific research as well . All of these make the Nanjing exhibition an increasingly progressing and greatly supported industry.The aim of InterExpo 2009 is to speed up the development of China’s exhibition industry and promote exchange and fair competition within the industry. InterExpo 2009 bases itself upon the market. It focuses on high quality and continues to operate in a professional mode to serve as an exchange and cooperative stage for companies at home and abroad to improve their images. It strives to establish and maintain good relation with customers.As an important event of the China Expo Forum for International Cooperation (CEFCO 2009), the Global Association of the Exhibition Industry (UFI), the International Association of Exhibition and Events (IAEE) and the Society of Independent Show Organizers (SISO), will hold the 10th China International Trade Show for Exhibition and Conference Industry (InterExpo 2009) at the Nanjing International Exhibition Center on Jan 14-16,2009. This will be jointly organized by China Council for the Promotion of International Trade. Show organizers, booth contractors , service suppliers, government bodies , exhibition media,etc, will once again gather together in Nanjing for the grand gathering of theconvention and exhibition industry.CAEC sincerely extends the invitation to you to participate in InterExpo 2009.Exhibitor Categories:1.Trade show and conference organizers, exhibition companies2.Exhibition and conference facilities, exhibition complex , convention centers, resorts3.Designers and contractors4.Equipment sppliers of display material and accessories5.Exhibition freight forwarders6.IT product and service , e-business service7.Associations of commerce, trade promotion organizationsernmental convention management bodies9.Business travel agencies ,hotels, airlines10.Trade show media ,magazines and web companies11.Service suppliers related to trade showGeneral Information:1.Moving In: January 1 th-13 th, 20092.Exhibition: January 14th-16th, 20093.Moving out: January 16h, 2009(after 14:00)1.Answer the following questions orally according to the passage.1) What is the full name of InterExpo 2009?2) Where is InterExpo 2009 held ?3) Who are the sponsors?4) Who are the organizers ?5) What is the theme of the InterExpo 2009 ?6) What is the exhibition aim of InterExpo 2009 ?7) How long does the convention last ?8) What does InterExpo 2009 exhibit ?2.Translate the following sentences using as many language skills learned in this lesson as possible .1) 主办单位2）承办单位3）协办单位4）你不仅可以在网上查到这次展会的主题和主旨，还可以在网上预订展位。

一、问候和介绍Welcome and Introduction (1)二、交谈语言Talking about Languages ........................................... 2.三、天气和环境Weather and Environment ........................................ 3.四、工程项目Engineering Project ............................................... 4.五、进度与计划Planning and Scheduling (5)六、技术资料和图纸Technical Documents and Drawings (7)七、公用工程Utility (9)八、施工现场On Construction Site (10)九、设备检查验收Inspection of the Equipment (12)十、土建工程Civil Engineering (14)十一、施工器械Construction Machinery (16)十二、常用工具Common Tools (17)十三、工程材料Engineering Material (19)十四、质量管理Quality Control ................................................ 2.2工程英语对话1. Welcome to China.欢迎你到中国来。

2. Welcome to our job site.欢迎你到我们工地来。

3. I wish we shall have a friendly cooperation in coming days.希翼今后友好合作。

4. Allow me to introduce myself, my name is…请允许我介绍自己，我的名字叫……5. Please allow me to introduce a fellow of mine, Mr.___.请允许我给你介绍一位我的同事，……先生。

精选英语作文范文10篇1innovationWith the economic globalization becoming increasingly intensive，invention or innovation has become one of central topic around the world. Many countries see it as the key to develop their economy. Our government，with no exception，regard it as “the soul of our nation.”What，however，can we benefit from it？First of all，invention or innovation can enhance overall national economy，which is important to heighten our nation status in the world. Additionally，if factories have the abilities in invention，the factories will be booming. As a result，they can provide our people with more jobs and it will make our nation more stable. What is more， a majority of new products may be avaible through invention. Therefore，it is also significant factor for improving our living standards. Owing to such benefits，no wonder more and more countries focus on invention today.Recognizing its importance is only the fist step to advocate invetion or innovation，and some effective measures should be taken for it. For one thing，our authority should continue to enlarge the recruit of graduate. For another，the conditions of scientists and skilled workers should be further improved. Only in this way，can “the soul of our nation”be fully embodied and our nation have a brighter future.2InternetInternet is becoming more and more popular in the world. As is shown in the pictures，two persons are sitting in front of their own computer in different places. they seem to be very happy owing to the accession to Internet. We can see clearly that the left fellow（guy）types a message and sends it to the right one，the message says：“David，would you have a coffee tonight？”the right one receives it and quickly replys：“sure，where？”What a convenient tool of Internet is！With the rapid development of high technologies and economy，people will get more and more benefits from the use of internet. There is no denying that computer network is an indication of social civilazation and progress. internet is an information pool ，people can search anything they want by typing the key words. For example，someone wants to know the Great Wall of China，what he/she need to do is only to open an internet exporer and type the words：“the Great Wall”and search it，then he/she can get so many relevant links. What‘s more，people can purchase their favorite goods through the Internet instead of going shopping in chaotic st but not least，people also can make full use of Internet to study online . From the analysis made above，we can come to a conclusion that we should make the best use of Internet，which can brings us a great deal of benefits. as a citizen of China we should study hard. Only in this way Can we have a brighter future and make our country flourishing。

芯片公司祝福语英文As a chip company, we understand the importance of expressing our heartfelt blessings to our clients, partners, and employees in a sincere and meaningful way. In today's fast-paced and competitive business environment, it is crucial to maintain strong relationships and show appreciation for the support and collaboration we have received. Therefore, we have compiled a list of heartfelt and genuine blessings in English to convey our gratitude and best wishes to everyone who has been a part of our journey.To our clients, we extend our warmest blessings for continued success and prosperity. Your trust and confidence in our products and services have been the driving force behind our growth and innovation. We are truly grateful for the opportunity to serve you and look forward to building even stronger partnerships in the future.To our partners, we express our sincere appreciation for your unwavering support and collaboration. Together, we have achieved remarkable milestones and overcome challenges that have strengthened our bond and contributed to our mutual success. May our partnership continue to thrive and bring forth new opportunities for growth and development.To our employees, we offer our heartfelt blessings for health, happiness, and fulfillment in both personal and professional endeavors. Your dedication, talent, and hard work are the foundation of our company's achievements, and we are grateful for your contributions to our collective success. May you continue to excel and inspire others with your passion and commitment.In the spirit of the holiday season, we extend our blessings to all those who have been a part of our journey. May the coming year be filled with joy, prosperity, and new opportunities. Let us continue to work together, learn from each other, and create a brighter future for all.As we reflect on the past year and look forward to the future, let us embrace the spirit of gratitude and generosity. Let us strive to build stronger relationships, foster greaterunderstanding, and create a positive impact in the world around us. Together, we can achieve remarkable things and bring about positive change in our industry and beyond.In closing, we offer our heartfelt blessings to all those who have supported and believed in us. May the coming year be filled with success, happiness, and fulfillment. Let us continue to work together, support each other, and make a positive difference in the world.Thank you for being a part of our journey, and may our blessings bring warmth and joy to you and your loved ones.。

北京外企邮件英文感谢语1. Thank you for your prompt response to my email.2. I appreciate your assistance with the project.3. Thank you for your kind support during my time at the company.4. I wanted to express my gratitude for the opportunity to work with sucha talented team.5. I am truly grateful for the guidance and mentorship you have provided.6. Thank you for your patience and understanding.7. Your expertise and dedication have been invaluable.8. I wanted to express my sincere appreciation for your continued support.9. Thank you for going above and beyond to meet the deadline.10. I am grateful for the opportunity to collaborate with you on this project.11. Thank you for your attention to detail.12. I wanted to extend my heartfelt thanks to you for your assistance.13. I am grateful for the valuable feedback you provided.14. Thank you for your professionalism and commitment to excellence.15. I wanted to express my appreciation for your hard work and dedication.16. Your support has been instrumental in the success of this project.17. I am truly thankful for your understanding and flexibility.18. Thank you for your trust and confidence in my abilities.。