不同采收期的罗布麻叶和其商品中槲皮素的含量测定

HPLC测定罗布白麻叶中的芦丁和槲皮素
郑虹
【期刊名称】《华西药学杂志》
【年(卷),期】2008(23)6
【摘要】目的建立测定罗布白麻叶中芦丁和槲皮素含量的方法。

方法采用HPLC 法,色谱柱为ZorbaxSB-C18(250mm×4.6mm,5μm),流动相为甲醇-0.04%磷酸(40:60),流速为1.0ml·min-1,检测波长为360nm,柱温为35℃。

结果芦丁和槲皮素的线性范围分别为0.01～0.20μg和0.007～0.099μg;r分别为0.9999和0.9998;平均回收率分别为102.1%(RSD=2.0%,n=6)和99.5%(RSD=1.4%,n=6)。

结论所建方法快速、简便、准确、重复性好,可作为罗布白麻叶药材质量控制的有效方法。

【总页数】2页(P727-728)
【关键词】高效液相色谱法;罗布白麻叶;芦丁;槲皮素
【作者】郑虹
【作者单位】青海省药品检验所
【正文语种】中文
【中图分类】R917
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2.毛细管电泳法测定罗布麻叶中芦丁、异槲皮苷和槲皮素的含量 [J], 李芳;马晓丽;
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3.不同采收期的罗布麻叶和其商品中槲皮素的含量测定 [J], 章一菡;卞艳芳
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因版权原因，仅展示原文概要，查看原文内容请购买。

不同产地不同采收期中仙鹤草中槲皮素成分含量的分析比较研究目的：应用HPLC法对不同产地不同采收期的仙鹤草药材中槲皮素含量进行分析比较。

方法：采用HPLC法，色谱柱：Thermo BDS Hypersil-C18柱（Part No：28105-254630；4.6 mm × 250 mm，5 μm ），流动相为乙腈-甲醇-0.2%磷酸，检测波长为368nm，柱温30℃，流速1.0ml/min。

结果：槲皮素在0.108～2.160μg （R2=0.9998）范围内呈良好线性关系；平均加样回收率为98.82% （RSD=0.81）；不同采收期的仙鹤草药材中槲皮素的含量差别较大，其中八月份采收的样品中槲皮素含量最高；不同产地的仙鹤草药材中槲皮素含量有明显差别，其中以浙江湖州产含量最高。

结论：仙鹤草药材采收时间的不同，其中的槲皮素成分会发生很大变化，八月份为仙鹤草药材的最佳采收期；不同产地仙鹤草中槲皮素含量差别很大，说明仙鹤草有效成分槲皮素受地域的影响很大；该方法精密度高、重复性好、稳定性好，能够准确测定仙鹤草中槲皮素成分的含量，结果表明不同产地的仙鹤草差别较大，为仙鹤草的质量控制提供依据。

标签：不同产地；仙鹤草；槲皮素；比较研究Determination Comparison of Quercetin in Agrimoniae from Different Areas and Different Harvest Time【Abstract】Objective：To analyze the determination of quercetin in agrimoniae from different place of produce by HPLC method. Method：HPLC conditions included Thermo BDS Hypersil-C18 column （Part No：28105-254630；4.6 mm ×250 mm，5 μm）and the mobile phase was a mixture of acetonitrile，methanol and 0.2% phosphoric acid for gradient elution. The wavelength was 368nm and the column temperature was at 30℃. The flow rate was 1.0 ml/min. Results：Quercetin had good linear relationship in the range of 0.108～2.160μg （R2=0.9998），its average recoveries was 98.82% （RSD=0.81%）；Contents of quercetin in agrimoniae from different areas and different harvest time were of much difference，while agrimoniae from Huzhou of Zhejiang and harvesting at August had higher content. Conclusion：Along with the change of harvesting time and places of produce，the contents of quercetin in agrimoniae were much different. The best harvest time was August and the better place for planting agrimoniae was Huzhou，Zhejiang Province. This method was steady with high precision and good repeatability. It could be used for the determination of quercetin and quality control of agrimoniaet.【Keywords】different place of produce；agrimoniae；quercetin；analysis and comparison仙鹤草Agrimonia Pilosa Leded是蔷薇科植物龙牙草Agri monia pilosa Ledeb 的干燥地上部分，主产于浙江、江苏、湖北、黑龙江等地，此外，安徽、福建、广东、河北、山东、辽宁等地亦产[1]。

・基础研究・不同产地罗布麻叶UPLC特征图谱及黄酮类成分含量测定A吴淑珍，何民友**,李国卫，吴文平，邱韵静，曾荟，潘礼业广东一方制药有限公司广东省中药配方颗粒企业重点实验室，广东佛山523204［摘要］目的：建立不同产地罗布麻叶药材超高效液相色谱法(UPLC)特征图谱，并测定2个黄酮类成分的含量。

方法：采用Waters CORTECS T3型色谱柱(100mmx2.8mm, 1.7pu),乙腈-28%磷酸为流动相进行梯度洗脱，流速为0.8mL-min-1,进样量为1p L,柱温为35t,检测波长为360nm,建立16批罗布麻叶UPLC特征图谱，并进行相似度、聚类分析和主成分分析；同法测定药材中2种黄酮类成分(金丝桃苷、异槲皮苷)的含量。

结果：建立的6批罗布麻叶药材特征图谱共标定16个共有峰，相似度为0.287-0.999；聚类分析结合主成分分析结果表明，不同产地药材整体质量不太稳定；对金丝桃苷和异槲皮苷进行了含量测定，结果1批药材中2个成分的总含量差异不大，以安徽省阜阳市和河南省驻马店市产罗布麻叶药材黄酮类成分含量较高。

结论：建立的UPLC特征图谱结合含量测定及相似度评价、聚类分析和主成分分析评价方法，能全面地用于罗布麻叶药材的质量评价。

［关键词］罗布麻叶；特征图谱；含量测定；金丝桃苷；异槲皮苷；主成分分析；聚类分析［中图分类号］R232［文献标识码］A［文章编号］6732890(202］)0426628dog10.83313/4.issn.6732890.2016160500］UPLC Charrcteristie Mapt and Determination of Flavonoibt io Apocyni Venehl Fobvmfrrm Differeel Prrducina AreetWU Shu-zhen,HE Min-yo*,LI Guo-wei,WU Wen-pmg,QIA Yun-jmg,ZENG Hui,PAN Li-ye0110^01^Pmiacial Key Laboratory W Traeitional Chinese Formula Graauh,Guangeoiig Yifan Pharmaceidcal Co.,LO.,Foshan528244,China7Abstrect］Objechvv:The UPLC characterisPo mags of Apocyni Veneti Folium from diherent rexions were estaUUshep,and the c ontents of two^▼(^(:^)were determineX to provide a reference foi the quality control of this medicine.Method:Waters CORTECS T3column(100mm x2.1mm, 1.7p m)was useX for the analysis with acetonitrile-0.1%phosphoric acid us the modile phase for gradient elution,U ow rate0.8mL*min_1,injection volume1pL,columntemperature32t,detection wavelength394nm.The fingerprints of6batch samples was estaUPsheX,and similarity,cgster analysis and principal component analysis were carrieX out The content of two Uavonoib components(hyperidin and nsoqunetnteen)wasdntenemnndbptehnsamnmntehod.Retuait：Thn16batethnsoeApotpnVnntenFo.num thaeattenenstentmaps were caPbrateX with6common peads with a similarity of0.9862.999；clpster analysis combineX with princidai component analysis showeX that the overall quality of the meXicinal materials from different origins was not stable.The content of glycosides was determineX,and the results showeX that the total content of6batches samples was not significantly different.The content of^▼(^(:^)in Apocyni Veneti Folium from Fuyang city in Andui province and Zhumadian city in Henan province was hmUer.Conclusion:The UPLC characterisPo map estaUPsheX in this stupy,combineX with content determination and similarity evaluation,cluster analysis and principal component analysis evaluation methods,can be comprehensiveg useX foi the quality evaluation of Apocyni Veneti Folium.A［基金项目］广东省中药配方颗粒工程实验室项目(粤发改创新函〔2018〕3149号)；广东省省级科技计划项目(2018B030323492)*［通信作者］何民友，主管中药师，研究方向：中药质量标准研究；Tel：(0757)85128602,E-mail：675844585@qq.cm・610・[Keywoolt]Apocyni Veneti Folium；characteristic map；content determination；hypekcin；isoquercetin；principal component analysis；cluster analysis罗布麻叶为夹竹桃科植物罗布麻Aocynnm venetum L.的干燥叶，为夏季采收，除去杂质，干燥。

不同采收期的罗布麻叶和其商品中槲皮素的含量测定章一菡;卞艳芳【期刊名称】《药学实践杂志》【年(卷),期】2017(035)001【摘要】Objective To determine the quercetin content in Folium A pocyni Veneti from different commercial markets and harvest time byHPLC .Methods The determination was performed on an Aglient Eclipse XDB-C18 column (250 mm × 4 .6 mm ,5 μm)at 30 ℃ with a mobile phase composed of Methanol :0 .1% phosphoric acid run at gradient elution ;the detec-tion wavelength was 360 nm .Results It is a better timing to harvest when the flower is turning fruit and the leaf color is turn-ing from green to yellow .Our results have demonstrated that at that time ,the content of quercetin in Folium A pocyni Veneti was relatively higher .In addition ,this report also shows that the concentration of quercetin in most medicinal materials from domestic market complies with the requirement of the Pharmacopoeia ,but there were still a small percent of samples that didn′t meet the Pharmacopoeia standards .Conclusion We suggested that the quality supervision and inspection on Folium A pocyni Veneti needs to be strengthened .%目的：建立HPLC法测定不同采收期的罗布麻叶和其商品药材中槲皮素的含量。

基金项目:江苏省中药炮制重点实验室开放式课题项目(ZYPZ007);江苏省中医药管理局资助项目(LZ09020);盐城市2008年科技发展计划项目(YK2008081)作者简介:刘训红,男,教授 研究方向:中药品质评价研究 Tel:(025)85811511 E 2mail:liuxunh1959@s ohu 1com HPCE 2DAD 同时测定罗布麻叶中4种黄酮的含量刘训红,张月婵,李俊松,王媚,王丽娟(南京中医药大学药学院,南京210046)摘要:目的　建立高效毛细管电泳二极管阵列检测法(HPCE 2DAD )同时测定不同产地、采收期和种属的罗布麻叶中芦丁、异槲皮苷、金丝桃苷及槲皮素含量的方法。

方法　选择毛细管区带电泳分离模式,以45mmol ・L -1硼砂缓冲液(pH 912)为电泳介质,未涂渍标准熔融石英毛细管(6415c m ×75μm,有效长度56c m )为分离通道,分离电压为25k V,检测波长为206n m,毛细管温度为25℃,压力进样为5000Pa,5s 。

结果　4种黄酮的峰面积与浓度的线性关系良好(r >019986);加样回收率为95134%～102165%。

结论　该方法简单、准确,重现性较好,可用于罗布麻叶药材内在质量的评价和控制。

关键词:高效毛细管电泳二极管阵列检测法;罗布麻叶;芦丁;异槲皮苷;金丝桃苷;槲皮素中图分类号:R284 文献标识码:A 文章编号:1001-2494(2010)06-0464-04S im ult aneous D eterm i n a ti on of Four Fl avono i ds i n Foli u m Apocyn i Veneti by HPCE 2DADL IU Xun 2hong,Z HANG Yue 2chan,L I Jun 2s ong,WANGMei,WANG L i 2juan (Phar m acology College of N anjing U niversityof Chinese M edicine,N angjing 210046,China )ABSTRACT:O BJECT I VE To establish a high perf or mance cap illary electr ophoresis method with di ode array detecti on (HPCE 2DAD )for si m ultaneous deter m inati on of rutin,is oquercitrin,hyperin and quercetin in foliu m apocyni veneti fr om different habitats,har 2vesting ti m e and s pecies 1M ETHOD S Based on the mode of cap illary zone electr ophoresis,45mmol ・L-1borax was used as buffers oluti on (pH 912),uncoated fused silica cap illary (6415c m ×75μm id )was used,separati on voltage was 25k V ,detecti on wave 2length was at 206n m,colu mn te mperature was maintained at 25℃,and sa mp le was injected at 5000Pa f or 5s 1RESUL TS Four fla 2vonoids showed good linearity (r >019986)in the range of the tested concentrati on 1The average recoveries of the method were in the rang of 95134%-102165%1CO NCL US I O N The method is si m p le,accurate and rep r oducible,and can be used for quality contr ol of Foliu m Apocyni Veneti 1KE Y WO R D S :HPCE 2DAD;f oliu m apocyni veneti;rutin;is oquercitrin;hyperin;quercetin 罗布麻叶为中国药典收载的常用中药,系夹竹桃科植物罗布麻(A pocynum veneto m L.)的干燥叶,具平肝安神,清热利水之功效[1],其药用、茶饮历史悠久。

HPLC 法测定维吾尔药大花罗布麻叶中异槲皮苷的含量穆建;佟瑞敏;苏来曼·哈力克【摘要】Objective To establish a method for the determination of isoquercitrin in leaves of Pocvnum hendersonii (Hook.f.)Woodson by HPLC.Methods HPLC method was adopted for a quantitative analysis of isoquercitrin.It was carried out on C18 column,with the mobile phase of methnal-acetonitrile-0.3% phosphoric acid water (11∶ 10∶ 79),the flow rate of 1.00 ml/min,the column temperature of 35℃ and the wavelength for detection of 354 nm.Results There were good linear relationships between the peak area and concentration at the range of 0.020 2~0.202 mg/mL for isoquercitrin (r =0.999 9).The average recovery rate of isoquercitrin was 104.45% (n =6),with RSD of 0.45%.The average content of ten different samples was 0.37%.Conclusion HPLC method to determine the isoquercitrin in leaves of Pocvnum hendersonii (Hook.f.)Woodson was proved to be simple,accurate and reproducible,which can provide scientific basis for quality control of leaves of Pocvnum hendersonii(Hook.f.)Woodson.%目的：建立高效液相色谱法测定大花罗布麻叶中异槲皮苷含量的方法。

龙源期刊网
罗布麻不同药用部位有效成分的含量考察
作者：傅正良赵连兴孔增科毕雪艳贺伟丽
来源：《中国民族民间医药·下半月》2010年第06期
【摘要】目的:研究罗布麻不同药用部位有效成分的含量与药用价值。

方法:通过薄层色谱法、紫外分光光度法对罗布麻不同药用部位有效成分的含量进行研究。

结果:罗布麻各药用部位紫外吸收在204±2nm处有最大吸收;薄层色谱分析除罗布麻茎(2)、罗布麻根(3)外均在Rf值0.64、0.52处有翠绿色斑点;高效液相含量测定结果:罗布麻叶中槲皮素含量均>中国药典规定的0.60%,罗布麻花、茎、根、全草中槲皮素的含量均
【关键词】罗布麻;罗布麻叶;槲皮素;含量;鉴别
【中图分类号】R284.1【文献标识码】A【文章编号】1007-8517(2010)12-045-2。