AQP-4蛋白和AQP-4mRNA在新生鼠缺氧缺血脑组织的表达变化

新生大鼠缺氧缺血性脑损伤AQP-4表达及依达拉奉治疗效果的研究目的:探讨新生大鼠缺氧缺血性脑损伤(hypoxic-ischemic brain damage,HIBD)后脑组织水通道蛋白-4(AQP-4)的表达变化及依达拉奉(edaravone)对其治疗效果的影响。

方法:将7日龄Wistar大鼠120只随机分为3组:假手术组、缺氧缺血模型组(HIBD组)及依达拉奉治疗组,每组各40只。

每组根据处死的时间不同又分5个亚组:6、24 h、3、5、7 d组,每亚组各8只,各组分别于不同时间点处死动物,测脑组织含水量,并采用免疫组化方法检测AQP-4的表达。

结果:AQP-4在缺氧缺血6 h即增强,3 d达高峰,5～7 d逐渐降低(P＜0.01);治疗组各时间点AQP-4表达水平较HIBD组明显降低(P＜0.01)。

结论:AQP-4参与了HIBD脑水肿的发生发展过程,依达拉奉可降低AQP-4的表达,从而减轻脑水肿,这可能是其脑保护作用机制之一。

[Abstract] Objective: To probe into the expression of AQP-4 in brain tissue after hypoxic-ischemic brain damage(HIBD) in neonatal rats and the effect of Edaravone on the treatment of HIBD. Methods: A total of 120 seven-day-old Wistar rats were randomly divided into three groups(n=40), sham-operated group, hypoxic-ischemic brain damage(HIBD) group and Edaravone-treated group. Each group were divided into five sub-groups(n=8 in each subgroup) based on different time points after HIBD(6,24 h,3,5,7 d). Animals were sacrificed at different time points. Changes of brain water content were determined. And immunohistochemical technique was used to determine the expression of AQP-4. Results: The expression of AQP-4 increased significantly on 6 h after HIBD, the peak reached on 3 d and 5-7 d gradually decreased(P＜0.01). The level of AQP-4 expression in treatment group was significantly lower than HIBD group at each time point(P＜0.01). Conclusion: AQP-4 involves in the occurrence and development process of cerebral edema after HIBD. Edaravone can reduce the expression of AQP-4, so as to alleviate brain edema, which may be the one of the mechanisms of brain protection.[Key words] Edaravone; Aquaporin-4; Neonatal rats; Hypoxic-ischemic; Brain edema新生儿缺氧缺血性脑病(hypoxic-ischemic encephalopathy,HIE)是由于各种围生期窒息导致胎儿或新生儿脑缺氧缺血性损伤,而脑水肿是新生儿HIE早期出现的病理生理过程,与许多后遗症的发生密切相关。

AQP4及AQP4 mRNA在重型脑损伤中脑脊液中的表达及意义谯明;彭艳;杨汉兵;雷振富;王俊英;彭雪刚;文刚【摘要】目的观察重型脑损伤后脑脊液中水通道蛋白4(AQP4)及AQP4 mRNA 的表达变化及意义.方法选取重型颅脑损伤患者51例,对每例患者第0、3、7天脑脊液进行AQP4 ELISA(酶联反应吸附实验)含量测定,RT-PCR(实时定量聚合酶链式反应)测定AQP4 mRNA表达.结果第0、3、7天脑脊液中均可检测AQP4及AQP4 mRNA.第3天较第0天升高(P<0.05),第7天较第3天降低(P<0.05),第7天与第0天比较,差异无统计学意义.AQP4与AQP4mRNA相关(r=0.867).结论重型脑损伤后患者脑脊液AQP4的表达随着病程变化,推测AQP4可能参与了脑损伤的病理过程,与病情的转归相关.%Objective To observe the change of aquaporin (AQP)4 and AQP4 mRNA level in the cerebrospinal fluid of patients with severe brain injury and its clinical significance.Methods There are 51 severe traumatic brain injury patients. ELISA (enzyme-linked immunosorbent assay) was used to determine cerebrospinalfluid AQP4 protein expression after 0,3,7 days,and RT-PCR (reverse transcription PCR) was used to determine AQP4 mRNA expression.Results AQP4 protein or mRNA expressed within the 0,3,7 days.The level of AQP4 or AQP4 mRNA increased 3 days after operation (P <0.05),and then statistically decreased 7 days after operation (P <0.05). But the 7 days had no statistically difference compared with 0 day after operation.Conclusion After Brain injury, AQP4 expression changes after brain injury is closely relatedwith theformation of cerebral edema, AQP4 in the damage brain edema formation plays an important role.【期刊名称】《当代医学》【年(卷),期】2015(021)034【总页数】3页(P16-18)【关键词】重型脑损伤;水通道蛋白4【作者】谯明;彭艳;杨汉兵;雷振富;王俊英;彭雪刚;文刚【作者单位】四川 620500 眉山市第二人民医院(仁寿县人民医院)重症医学科;四川 620500 眉山市第二人民医院(仁寿县人民医院)重症医学科;眉山市第二人民医院(仁寿县人民医院)神经外科;眉山市第二人民医院(仁寿县人民医院)检验科;四川620500 眉山市第二人民医院(仁寿县人民医院)重症医学科;四川 620500 眉山市第二人民医院(仁寿县人民医院)重症医学科;四川 620500 眉山市第二人民医院(仁寿县人民医院)重症医学科【正文语种】中文水通道蛋白（aquaporins）有一个六跨膜α-螺旋结构域和共有天冬酰胺-脯氨酸-丙氨酸基序，它构成了孔的一部分，在动植物及微生物细胞中广泛分部的细胞膜转运蛋白，与水通透性相关。