New MARPOL Annex II Chem Code Handout

Pentra DX NexusProcess efficiency in HematologyComfort•Large color touch scr een.•Intuitive interface with virtualkeyboard.Ergonomics•Smart access to all functionalities.•Enhanced visibility.•Compr ehensiveicons.External links•USB port for multi-data exchange and hardwareconnection.•120 samples per hour •28 parameters•Automatic r eflex testing •Automatic validation of results •Integrated cytology atlas •SPS evolutionPentra DX NexusBe productive and flexibleQuality•Complete traceability for each run including identification, lot number and expiration date on r eagents andcontrols.Compatibility•Compatible racks with most pr e-analytical systems and post analytical systems.•Two models ofracks.Environment•New laser source r equi re less space, r educing heat and noise.(1) Laser LED (2) Photomultiplier (3) Hyd r ofocalisation (4) Impedancemetry (5) Optical mesur ementHematopoiesisthroughPentra DX RangeSCIENTIFIC BOOKReference methods CBC DIFF RET NRBC Impedancemetry ••••Flow cytometry •••••y r t e m o r o u l F Cytochemistry ••••SS H D (1)(2)(3)(4)(5)LMNE matrixDHSS ConceptFull leukopoiesis analysis:Flow cytometry Injection of the sample prepared in a double hydrodynamic flowcytometer (HORIBA Medical patent),and determination of the cell complexity by measuring the absorbance of a polychromaticlight source, or the fluorescence by fluorocytometry (Diode type laser at 488 nm wavelength).CytochemistryIncubation of the sample at a regulated temperature andcells stained with Chlorazol Black.This reagent stains specifically leukocyte cytoplasm, granules,and nuclei.• Matu re populations 5 DIFF • Reduced slide r eview• Diagnostic and follow-up tool for rapid decision-makingErythroblastsFluorescence-based count:•Eryth r oblast analysis in r outine or r eflex mode based on:- inte r nal laboratory rules - detection alarms- patient demographics...•Use of Thiazole Orange fluo rochrome•Double Hyd r odynamic Sequential System (HORIBA Medical Patent)ReticulocytesDifferential diagnosis of anemia:• Classification and monitoring of anemia based on the Reticulocyte count and the CRC (Corrected Reticulocyte Count)• Follow-up of i r on-deficiency anemia based on the MR V (Mean Reticulocyte Volume)• Detection and monitoring of the eryth r opoietic r esponse accor ding to three stages of maturation: RET High, RET Medium, RET Low and maturation parameters (IR F , MFI, and MR V)Erythroblasts ReticulocytesRed blood cellsSPS EvolutionPerfect standardization of blood smears•Integrated slide-maker*120 slides per hourFully automated and secured process•Choice of staining protocolsMay-Grünwald Giemsa, W right, W right Giemsa,etc.•Sampling volume: 50 µl of whole blood (additional) On primary closed tube •Positive identificationBarcode r eadingPatient data printed automatically on slide •Smearing flexibilityUser-defined smearing pr ofiles based on the laboratory’s own rules*Optional moduleExpert validation station Results validation•Full management of samplesAlarms trigger ed accor ding to patient pr ofile Delta checkPr ogrammable r erun and r eflex test rules •Automatic validationStandardization of Lab rules (integrated catalog) Inte r national r ecommendation rules for Hematology r eview (ISLH,Laboratory Hematology11:83-90 © 2005 Car den Jennings Publishing Co.,Ltd.doi:10.1532/LH96.05019) integrate d in r outineAutomatic validation based on rules,flags,patient history,etc.•Integrated cytology atlas: HematovisionAid to accurate diagnosis supportExcellent education tool •Quality assuranceContr ol blood managementGraphical and table statisticsXB managementQCP Export (Quality Cont rol Program)Complete traceability (r eagents & cont rols) for eachrunPentra DX NexusT echnical SpecificationsPHYSICAL SPECIFICATIONSDimensions & WeightHeigh t W idth Depth W eightW ithout SPS73 cm120 cm55 cm110 kg28.7in47.2in21.6in242.5lbW ith SPS73 cm170 cm55 cm170 kg28.7in66.9in21.6in374 lb PrinterLaserThroughputUp to 120 samples/hour in CBC, DIFF,CBR, SPS modesUp to 60 samples/hour in DIR,ERB, CBE modesOperating temperature16- 34°C (61-93°F) room temperatureSpecimen volumeManual cycle 130 µLAutomatic cycle 200 µLPower requirementsPower supply from 100 V AC to 240 V AC (± 10%)50 Hz to 60HzPower consumption Pentra DX120 900 V AReagentsABX DiluentABX L ysebio(cyanide free)ABX FluocyteABX Leucodi ffABX BasolyseABX CleanerPARAMETERSCBCWBC RBC HGB HCT MCV MCH MCHC RDW PLT MPVDifferential LeukocytesNEU# & NEU% L YM# & L YM%MON# & MON% EOS# & EOS% BAS# & BAS% ReticulocytesRET%RET#RETH%RETM%RETL%IMM%CRC%IRF%MRV ErythroblastsERB%ERB#SOFTWARE SPECIFICATIONSData ProcessingColor LCD:12.1inCapacity:90,000 resultsOperating System: W indows XP Embedded™Pr ocessor:Genuine Intel1.60GHzRAM(Random Access Memory): 1 GBExter nal DVD/CD drive connected to instrument USB portRS232C, 5 X USB1User definedflagging limitsT ransmit patient & QC to LISMono & bi-di r ectional connectionsQuality Control Management48 selectable QC filesXB: 100 operator selectablefiles with statistics (20 samples per file) W ith-in runLevey-Jennings graphsLogsReagents,quality cont r ol,calibration,maintenance, user, settings,communication, err ors,blanksPERFORMANCE DATALinearityParameters Standa rd UnitWBC0.1- 15010/mmRBC0.01 - 810/mmHGB0.65 - 24 g/dLHCT 1 - 67%PLT 6 - 190010/mmPLT (platelet concentrated) 6 - 280010/mm Precision:Parameters Rang e Unit s% CV WBC 4.0-10.010/mm< 2 RBC 3.6-6.210/mm< 2 HGB12.0-18.0g/dL< 1 HCT36- 54% < 2 PLT150 - 50010/mm< 5 NEU%45 - 80%< 3L YM%25 - 50%< 5 MON% 2 - 10%< 10 EOS% 1 - 5%< 20 BAS%0.5-2.5%< 30CERTIFICATIONEN 61326: 2001IEC 61000-3-2: 2000IEC 61000-3-3: 2001IEC 61010-1: 2001IEC 61010-2-081: 2001IEC 61010-2-101: 2002CE 98/79/EC949 453 0500 • 888 903 5001/medical A D -0 4 0 9 1 3 -1 R e v . 1。

构象分析•如果分子中原子的两种不同空间分布仅通过键的自由转动即可互变，那么这两种立体异构叫构向（conformation）；若采取这种方式不能互变的叫构型（configuration）。

构型异构体可以被分离，构象异构体之间快速互变，因而不能被分离。

很多方法被用于测定构象如射线和电子衍射外紫外拉曼•很多方法已经被用于测定构象，如X‐射线和电子衍射、红外、紫外、拉曼、NMR和微波光谱、光电子能谱、超声波分子喷射光谱和光旋转色散及CD。

这些方法中有些方法只适于测量固体，分子的构象在固态中不一定与溶液中相同。

•分子构象可以通过分子力学方法计算。

开链体系构象•对于连接两个sp3碳原子的开练单键，可能有无穷多的构想，每种构想伴有一定的能量，而实际情况中，构想的数目要小很多。

乙烷有两个极限构象，即高能构象低能构象构象法描架最高势能构象和最低势能的构象。

这些构象可用两种方法描述：锯架式和Newman投影式。

•交叉式是乙烷最低的构象，重叠式则最高，最高构象与最低的能差约为2.9kcal/mol，这个能差被称为能垒。

扭转角是XCC所在平面与CCY所在平面间的二面角。

•对产生能垒的原因有多种解释，其中分子轨道计算得到的结论认为这种能垒是由于分子轨道的重叠引起排斥造成的。

•比氢大的基团会导致更高的能垒，当能垒足够高时，如适当取代的联苯或二金刚烷化合物，它们在室温下的转动完全被阻，因而它们间被称为构型关系，而非构象关系了。

1,2‐二取代乙烷构象•比乙烷稍复杂的情况是1,2‐二取代的乙烷，如正丁烷，有四种极限构象：全交叉是（反叠式）；邻位交叉式gauche 四种极限构象全交叉是（反叠式）邻位交叉式（顺错式synclinal）;全重叠式或顺叠式synperiplanar;部分重叠式或反错式anticlinal.•重叠式的构想不常见，只是作为从一种交叉式到另一种交叉时构想的中间过程。

固态时通常只有一种构象异构体。

•注意：丁烷及类似1,2‐二取代分子的邻位交叉式构象具有手性。

专利名称：NOVEL MICROPEPTIDE HMMW ANDAPPLICATION THEREOF发明人：XU, Hanmei,LI, Mengwei申请号：EP20860427.2申请日：20201103公开号：EP4006048A2公开日：20220601专利内容由知识产权出版社提供专利附图：摘要：The present invention provides a micropeptide HMMW of a new structure and an application thereof, and relates to the field of biomedical research and development.The micropeptide HMMW is obtained by encoding human IncRNA, and a recombinantvector is constructed so that objective cells perform high expression on the micropeptide HMMW, which can inhibit proliferation and migration of multiple solid tumors including the head and neck cancer, thyroid cancer, lung cancer, esophageal squamous cellcarcinoma, stomach cancer, breast cancer, kidney cancer, skin cancer and the like, and growth of tumors in the body. The micropeptide HMMW has potential value for new drug development, important tumor detection and treatment value.申请人：Nanjing Anji Biotechnology Co., Ltd.地址：6F HongFeng Technology Park A7 Economic-Technological Development Area Qixia District Nanjing, Jiangsu 210038 CN国籍：CN代理机构：Kramer Barske Schmidtchen Patentanwälte PartG mbB更多信息请下载全文后查看。

诺贝尔化学奖揭晓，又有德国科学家哦
2021年诺贝尔化学奖揭晓。

德国科学家Benjamin List和美国科学家David W.C. MacMillan因为“在不对称有机催化方面的发展”被授予2021年诺贝尔化学奖。

两名获奖的科学家都比较年轻：
•Benjamin List于1968年出生于德国的法兰克福。

1997年在法兰克福大学获得博士学位。

目前为德国马普煤炭研究所研究人员。

•David W.C. MacMillan于1968年出生于英国贝尔斯希尔。

1996年在美国加州大学欧文分校获得博士学位。

目前为美国普林斯顿大学教授。

催化剂是化学家的基本工具，而通常的催化剂只有金属和酶两种。

Benjamin List和David MacMillan各自独立开发出一种精确的分子构建新工具——不对称有机催化——而被授予2021年诺贝尔化学奖。

这种催化是建立在小的有机分子上的。

催化剂参与各种反应制造各种材料，但是却不会成为最终产品的一部分。

无论是对汽车工业还是药物研究领域都充满划时代的意义。

他们的研究让化学变得环保。

诺贝尔化学委员会主席Johan Åqvist说:“催化的概念既简单又巧妙，事实上，许多人都想知道为什么我们没有更早地想到它。

”
Benjamin List在诺贝尔化学奖揭晓时，正在阿姆斯特丹和妻子度假中。

在早餐时接到了来自斯德哥尔摩的获奖电话通知。

他说，“我还以为是有人和我开玩笑呢。

我绝对没有想到，今天太让人开心了。

“。

垃圾记录簿GARBAGE RECORD BOOK船名：Name of ship船舶编号或呼号：Distinctive numberor letters国际海事组织编号：IMO number使用期自：至：Period from toIntroduction:根据《经1978年议定书修订的<1973年国际防止船舶造成污染公约>》附则V第10条，对船舶每一次排放操作或焚烧过程应有记录，包括向海中、向接收设施或向其他船舶的排放，以及垃圾的意外落失。

In accordance with regulation 10 of Annex V of the International Convention for the Prevention of Pollution from Ships, 1973, as modified by the Protocol of 1978 （MARPOL）, a record is to be kept of each discharge operation or completed incineration. This includes discharges into the sea, to reception facilities, or to other ships, as well as the accidental loss of garbage.2垃圾和垃圾管理Garbage and garbage management:垃圾系指产生于船舶正常营运期间并需要连续或定期处理的各种食品废弃物、生活废弃物、操作废弃物、所有的塑料、货物残留物、焚烧炉灰、食用油、渔具和动物尸体，但本公约其他附则中所界定或列出的物质除外。

垃圾不包括因航行过程中的捕鱼活动和为把包括贝类在内的鱼产品安置在水产品养殖设施内以及把捕获的包括贝类在内的鱼产品从此类设施转到岸上加工的运输过程中产生的鲜鱼及其部分。

70通常情况下，复杂的有机化合物是在实验室中经手工操作而成，可是由于在化合物科学文献中，许多反应步骤并没有特别准确的描述或者某些细节被人们忽略，最后导致一些合成步骤难以复制。

近日，《科学》杂志在线发布了英国格拉斯哥大学L e r o y Cronin教授发明成果——Chemputer： 化学编程语言驱动的模块化自动合成机器。

研究人员表示，Chemputer允许全世界的化学家将他们的配方转变成数字化密码，进而可以在线下载分享给其他人，就像下载音乐一样廉价和便捷。

实验中Chemputer 自动化系统，在无任何人为干预的情况下成功地合成了3种常用药物分子：盐酸苯海拉明(diphenhydraminehydrochlorid)，卢非酰胺(rufinamide)和西地那非(sildenafil)，三者的产率和纯度都堪比人工合成。

Chemputer系统由不同的模块构成，包括反应模块、内部过滤模块（可加热或冷却）、自动化的液-液分离模块以及溶剂蒸发模块，将化学合成的不同过程分成图标化的模块。

当然，合成化学物质不可能凭空制造——整个化学合成过程不仅需要Chemputer软件，同时也需要一些物理设备：瓶装试剂、圆底烧瓶、过滤和液-液分离装置、旋转蒸发器，以及输送化学品的管道、阀门和泵。

Leroy教授将文献中的合成方法和步骤指令输入到适合自动化的步骤，合成步骤被转换为数字代码，并可以在平台之间灵活地发布且无需修改，进而大大提高了重复性和复杂分子的合成率。

接下来，研究人员通过开发控制软件，在后续反应步骤中自动清洗和重复使用硬件模块，完成了一个从独立单元操作，到组合完整的多步骤合成过程，最终用于实验室规模的有机合成。

为确保Chemputer的正常运行，必须将定义、控制输入、反应器和输出等过程进行编程。

由此，Chemputer特定指令程序生成。

从抽象层面来通过Chemputer系统的数字化合成，有望推动药品研发领域的变革性发展。

Page 2 of 4 N885-13GB / 04.05ATTENTIONATTENTIONATTENTIONN885-13GB / 04.05 Page 3 of 4Alkaline Alkaline German English French earths ions earths ions hardnessppm hardness hardness mmol/lmval/l °d CaCO 3°e °f 1 mmol/l Alkaline earths ions 1,002,005,60100,007,0210,001 mval/l Alkaline earths ions 0,501,002,8050,003,515,001 German hardness 0,180,3571,0017,801,251,781 ppm CaCO 30,010,0200,0561,000,07020,101 English hardness 0,140,2850,79814,301,001,431 French hardness0,100,2000,56010,000,7021,00Conversion table:3Faults and their eliminationFaults can be detected in the following ways, for instance:●Variances from the allowed limits ●Rusty or silted cooling water ●Incrustation (e.g.lime deposits)●Pitting●Destruction of naturally formed protective layers.The underlying causes are often difficult to find.Various reactions are feasible depending on the cooling system itself, the materials contacting water, the cooling water temperature, pH value and the concentrations of the dissolved substances.The services of a specialist company for water treatment should be enlisted for those malfunctions which cannot be dealt with quickly.The ALD Vacuum Technologies AG project manager responsible for your plant is always available for consultation.4Cooling systems and their influence on the cooling waterThe facility's cooling water inlet and outlet are to be connected to the factory's cooling system.The facility is designed for non-pressurized outlet.Should backpressure be expected or the connection to a closed-circuit cooling system be planned the express consent of the facility vendor is required.It must have been clearly stipulated in the request to tender.The individual cooling circuits, the available monitoring systems and the inlet temperature, pressure and volume are shown in the cooling water diagram of the facility.4.1Fresh Water Once-through CoolingThe fresh, cold water flows directly through the facility's cooling circuits.The heated cooling water flows away into the sewage system without recooling.Water consumption is quite substantial and effective conditioning is difficult.The fresh water once-through cooling system must be examined continuously for corrosion and incrustation (e.g.lime deposits = poor heat conductivity), and be cleaned chemically whenever necessary .4.2Open-circuit Cooling SystemCooling water is recooled in an open-circuit installation such as a cooling tower.Part of the water is allowed to evaporate, thereby removing an adequate amount of thermal energy from the remain-ing cooling water.As a consequence of this process, the dissolved salts within the cooling water are concentrated further – drastically increasing its corrosiveness;any remaining free carbon dioxide tends to be driven out, while the oxygen content approaches the saturation level.Moreover, the residual calcium hydrogen carbonate (carbonate hardness) decomposes under the influence of warming and continued loss of carbon dioxide – into almost insoluble calcium carbonate (scale), leading to incrustation.As a result, the compulsory safeguard actions for open-circuit cooling systems include permanent monitoring of the permissible salt content, coupled with the administration of corrosion inhibitors,hardness stabilizers and biocides.All cooling water characteristics must also be controlled regularly.Page 4 of 4 N885-13GB / 04.054.3Quasi Closed-circuit Cooling SystemThe cooling water gives off a fraction of the absorbed thermal energy to a heat exchanger, flowing first through a minimal aerated equalization tank before returning to the system inlet.Only negli-gible water evaporation results.Incrustations are minimal and directly proportional to the volume of replenished cooling water.Oxygen can diffuse continuously into the cooling system water over the equalization tank, its concentration approaching the saturation level.Fouling caused by organic material can occur and lead to a build up of deposits.High salt concentrations, dissimilar metal parts and an unfavorable pH value promote internal corrosion processes.The quasi closed-circuit cooling system requires an effective inhibitor for corrosion protection.4.4Closed-circuit Cooling SystemThis cooling system is hermetically sealed and conveys the absorbed thermal energy to a heat exchanger.Thermal expansion of the cooling water is compensated over a diaphragm pressure ves-sel.Cooling water loss does not occur.The employment of pressurized closed-circuit cooling systems with double-walled vacuum instal-lations increases investment costs appreciably, as the inner wall of the vessel must be sufficiently reinforced to withstand the cooling system operating pressure.Using this system incrustation (lime deposit) is effectively stopped.Never the less, a yearly preventive maintenance check of the system using a water analysis is recommended to eliminate the risks of corrosion.。

Safety Data Sheet22 June 2016SDS-201-WQI-010 Rev. 0Safety Data Sheet22 June 2016 Trade name: 514-05 (0.5% NaCl Std. Solution)·vPvB: Not applicable.SDS-201-WQI-010 Rev. 0Safety Data Sheet22 June 2016 Trade name: 514-05 (0.5% NaCl Std. Solution)·Conditions for safe storage, including any incompatibilities·Storage:·Requirements to be met by storerooms and receptacles: No special requirements.·Information about storage in one common storage facility: Not required.·Further information about storage conditions: None.·Storage class:·Class according to regulation on flammable liquids: Void·Specific end use(s) No further relevant information available.Safety Data Sheet22 June 2016 Trade name: 514-05 (0.5% NaCl Std. Solution)SDS-201-WQI-010 Rev. 0Safety Data Sheet22 June 2016 Trade name: 514-05 (0.5% NaCl Std. Solution)SDS-201-WQI-010 Rev. 0Safety Data Sheet22 June 2016 Trade name: 514-05 (0.5% NaCl Std. Solution)SDS-201-WQI-010 Rev. 0Safety Data Sheet22 June 2016 Trade name: 514-05 (0.5% NaCl Std. Solution)HORIBA, Ltd. provides the information contained herein in good faith but makes no representation as to its comprehensives or accuracy. This document is intended only as a guide to the appropriate precautionary handling of the material by a properly trained person using this product. Individuals receiving the information must exercise their independent judgment in determining its appropriateness for a particular purpose.HORIBA, Ltd. MAKES NO REPRESENTATION OR WARRANTIES, EITHER EXPRESS OR IMPLIED, INCLUDING WITHOUT LIMITATION ANY WARRANTIES OF MARCHANTABILITY, FITNESS FOR A PARTICULAR P U RP O S E WITH RESPECT TO THE INFORMOTION SET FORTH HEREIN OR THE PRODUCT TO WHICH THE INFORMATION REFERS. ACCORDINGLY, HORIBA, Ltd. WILL NOT BE RESPONSIBLE FOR DAMAGES RESULTING FROM USE OF OR RELIANCE UPON THIS INFORMATION.·Department issuing MSDS: Water Quality Instruments division.·Abbreviations and acronyms:ADR:Accord européen sur le transport des marchandises dangereuses par Route(European Agreement concerning the International Carriage of Dangerous Goods by Road)IMDG:International Maritime Code for Dangerous GoodsDOT:US Department of TransportationIATA:International Air Transport AssociationACGIH:American Conference of Governmental Industrial HygienistsEINECS:European Inventory of Existing Commercial Chemical SubstancesNFPA:National Fire Protection Association(USA)HMIS:Hazardous Materials Identification System(USA)VbF:Verordnungüber brennbare Flüssigkeiten,Österreich(Ordinance on the storage of combustible liquids,Austria)·SourcesRTECSA chemistry large dictionary (KIORITZ CORP. publication 1963)A product safe data sheet preparation guide book (Corporate Juridical person Nippon Chemical IndustrialCo., Ltd. Organization information center) The MERCK indexSDS-201-WQI-010 Rev. 0。

洗衣粉物证的傅里叶变换拉曼光谱快速分析方法应用-应用化学论文-化学论文——文章均为WORD文档，下载后可直接编辑使用亦可打印——0引言洗衣粉是一种生活中常见的合成洗涤剂，有时也被凶案犯罪嫌疑人用于清理犯罪现场，在微量物证分析领域时常被当作可疑白色粉末要求鉴定分析。

洗衣粉主要由烷基苯磺酸钠、少量的非离子表面活性剂和一些助剂、荧光剂、酶等制作而成。

不同品牌和产地的洗衣粉在组成成分和加工工艺上的差异性，都会造成拉曼谱图的差异，这也是鉴别分析的依据。

本文应用傅里叶变换拉曼光谱分析技术对从市场上收集的常见品牌洗衣粉进行了检验研究，建立了洗衣粉物证的傅里叶变换拉曼光谱快速分析方法。

通过谱图分析初步实现了对不同品牌、不同产地洗衣粉的区分检验。

1实验部分1.1 样品来源与制备从各地收集13种不同品牌的洗衣粉，共计22个系列。

在显微镜下仔细分离并挑选颗粒较大的白色颗粒与彩色颗粒置于洁净载玻片上，样品无需前处理，直接检验。

1.2 实验仪器及条件Nicolet 5700傅里叶变换红外光谱仪（美国Thermo公司）、傅里叶变换拉曼光谱仪组件（美国Thermo公司）、光学显微镜（奥林巴斯）、Micro Stage（美国Thermo公司）白色颗粒：扫描次数200次，光谱分辨率8cm-1,测量范围4000-400cm-1.彩色颗粒：扫描次数次，光谱分辨率8cm-1,测量范围4000-400cm-1.2结果与讨论2.1 白色颗粒的区分根据各个样品的白色颗粒的傅里叶变换拉曼光谱图特征峰的差异，这些样品大致可分为8类。

Ⅰ类：拉曼特征峰出现在620cm-1附近。

Ⅰ类样品谱图如图1a 所示，拉曼振动峰较多，且在620cm-1附近有较强的特征峰。

Ⅰ类：荧光干扰较大，光谱基线较差。

Ⅰ类样品如图1b所示。

只有2#样品具有这样的谱图。

Ⅰ类：拉曼特征频率出现在1260cm-1附近。

Ⅰ类样品如图1c 所示，拉曼振动峰也很多。

该类样品在1600-1100cm-1有一系列明显的振动峰，其中的1260cm-1附近的振动峰是其他分类的样品不具备的。

澳洲茄边碱诱导胶质母细胞瘤U251细胞凋亡的机制研究赵祎博;张琳;傅若秋;曹梓珍;陈翔;陈剑鸿【期刊名称】《中国药业》【年(卷),期】2024(33)1【摘要】目的探讨澳洲茄边碱(SM)对胶质母细胞瘤U251细胞凋亡的影响及作用机制。

方法用SM(0,2,4,6,8μmol/L)处理细胞24 h,即溶剂对照组和SM 1组、2组、3组、4组。

采用CCK-8法和克隆形成实验分别检测细胞活力和增殖能力,并计算细胞存活率和克隆形成率;采用Annexin V-FITC/PI双染色法及流式细胞术检测细胞凋亡情况,并计算细胞凋亡率;采用蛋白免疫印迹(Western blot)法检测凋亡相关蛋白表达水平;采用2’,7’-二氯荧光素二乙酸酯(DCFH-DA)和JC-1荧光探针分别检测U251细胞中活性氧水平和线粒体膜电位。

结果与溶剂对照组比较,U251细胞存活率和克隆形成率随SM浓度的增加均呈降低趋势(P <0.05),细胞凋亡率呈升高趋势(P <0.01);经SM处理后,细胞剪切多聚腺苷二磷酸核糖聚合酶、剪切胱天蛋白酶3蛋白表达水平及细胞内活性氧水平均呈升高趋势(P <0.05),线粒体膜电位呈降低趋势(P <0.01)。

结论 SM可能通过激活线粒体介导的细胞凋亡通路,从而抑制胶质母细胞瘤U251细胞活性。

【总页数】5页(P35-39)【作者】赵祎博;张琳;傅若秋;曹梓珍;陈翔;陈剑鸿【作者单位】中国人民解放军陆军特色医学中心【正文语种】中文【中图分类】R932;R285.5【相关文献】1.澳洲茄碱诱导肺癌细胞株H446凋亡及其机制探讨2.澳洲茄胺诱导肠癌 HCT-116细胞凋亡的实验研究3.澳洲茄碱诱导胆管癌QBC939细胞凋亡及作用机制4.澳洲茄边碱诱导食管癌细胞KYSE150凋亡及其机制研究5.高效液相色谱法测定龙葵中澳洲茄碱与澳洲茄边碱含量的研究因版权原因，仅展示原文概要，查看原文内容请购买。