巴西橡胶树HbR3HP1的克隆及表达分析

巴西橡胶树Profilin基因克隆及生物信息学分析
李德军;刘向红;邓治
【期刊名称】《中国农学通报》
【年(卷),期】2011(27)30
【摘要】为了研究橡胶树中Profilin蛋白的功能,利用橡胶树Profilin基因部分已知序列设计引物,采用3′-RACE技术获得Profilin基因完整开放阅读框,该基因编码131个氨基酸,含有一个保守的PROF结构域。

在HbPro1蛋白中没有预测到跨膜区和信号肽,二级结构分析表明HbPro1属于混合型蛋白。

对25个Profilin蛋白系统进化分析表明,HbPro1在进化上具有保守性,属植物类Profilin蛋白家族。

HbPro1基因的克隆和生物信息学分析为深入研究其功能奠定了基础。

【总页数】5页(P187-191)
【关键词】巴西橡胶树;Profilin蛋白;基因克隆;序列分析
【作者】李德军;刘向红;邓治
【作者单位】中国热带农业科学院橡胶研究所农业部橡胶树生物学重点开放实验室;海南大学农学院
【正文语种】中文
【中图分类】S562
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巴西橡胶树生长素响应HbJAR1基因克隆与表达分析Gene Cloning and Expression Analysis of AuxinResponsive HbJAR1 in Rubber TreeLI Xiaona1，XIAO Houzhen3，WAN Sanlian4，ZHANG Dong2，ZHANG Yu2 *1 Institute of Scientific and Technical Information，CATAS，Haikou，Hainan *****，China2 Hainan Key Laboratory for Sustainable Utilization of Tropical Bio-resources / College of Tropical Agriculture and Forestry，Hainan University，Haikou，Hainan *****，China3 College of Materials and Chemical Engineering，Hainan University，Haikou，Hainan *****，China4 Sanya City Modern Agricultural Inspection and Warning Center for the Prevention and Control，Sanya，Hainan *****，ChinaAbstract JAR1，a auxin response gene，maintains dynamic balance of auxin concentration in plant，strengthens plant tolerance ability stress. To clarify the gene structure and function of JAR1 in rubber tree（Hevea brasiliensis Müll. Arg.），HbJAR1 gene was cloned in the leaves of rubber tree CATAS7-33-97 by RT-PCR. The deduced amino acid of HbJAR1 protein has special GH3 superfamily domain structure，no signal peptide，no transmembrane region，located in the cytoplasm. The gene expression of HbJAR1 in the leaves of rubber tree was significantly affected by light intensity，mechanical wounding and powdery mildew infection. IAA，GA3，ET，JA and ABA treatments alsoinduced HbJAR1 expression at different levels. The results suggested that HbJAR1 is a member of plant JAR1 gene family，involved in stress response such as light and wounding signal in rubber tree，etc.Key words Hevea brasiliensis Müll. Arg.；HbJAR1；gene clone；bioinformatics；expression analysisdoi 10.3969/j.issn.1000-2561.2017.08.017JAR1（*****TE *****NT 1）基因是植物生长素早期响应基因之一，这类基因与植物的生长发育密切相关。

巴西橡胶树棒孢霉落叶病菌Hog1同源基因的克隆和序列分析王延丽;林春花;时涛;李博勋;黄贵修【摘要】In order to understand the roles of hogl Gene in invasion process of Corynespora cassiicola into Hevea brasiliensis, hogl gene of CC01 was amplified by PCR and RT-PCR methods and the degenerate primers were designed according to hogl gene of several filamentous fungi. The amplification products were sequenced, the amino acid sequence were aligned and phylogenetic clustering analysis were done. Sequence analysis extrapolated that the open reading frame of this gene was 915 bp with 6 introns, encoding a putative protein of 304 amino acids. Phylogenetic clustering suggested that the sequence of CCHOG1 showed higher similarity to MAPK HOG1 of Pyrenophora tritici , MAP kinase C1K1 of Cochliobolus lunatus.%为了解MAPK信号途径中hog1基因在多主棒孢病菌(Corynespora cassiicola)侵染橡胶树过程中的作用,根据几种丝状真菌的hog1基因设计简并引物,采用PCR和RT-PCR的方法扩增巴西橡胶树棒孢霉落叶病菌CC01的hog1基因,并对其扩增产物进行测序,同时还对该基因编码的蛋白进行氨基酸序列比对和系统发育分析.结果表明:该基因开放阅读框架为915 bp,编码304个氨基酸残基,包含6个内含子；该基因的氨基酸序列与小麦黄斑叶枯病菌(Pyrenophora tritici)MAPK HOG1(XP_001935555.1)、谷子弯孢病菌(Cochliobolus lunatus)MAP kinase ClK1(AFJ42499.1)等高度同源.【期刊名称】《热带作物学报》【年(卷),期】2013(034)003【总页数】5页(P424-428)【关键词】多主棒孢;Hog1基因;基因克隆;序列分析【作者】王延丽;林春花;时涛;李博勋;黄贵修【作者单位】中国热带农业科学院环境与植物保护研究所农业部热带作物有害生物综合治理重点实验室海南省热带农业有害生物监测与控制重点实验室海南海口571101【正文语种】中文【中图分类】Q785由多主棒孢（Corynespora cassiicola）引起的橡胶树棒孢霉落叶病（Corynespora leaf fall disease，CLFD）现已成为南亚、东南亚和中非橡胶树最具破坏性的叶部病害，是继南美叶疫病之后第2个威胁世界天然橡胶生产的重要病害[1]。