微生物英文名词解释
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一、名词解释1.微生物:通常是一切肉眼看不见或看不清的,必须借助显微镜才能观察到的一群微小生物的总称。
2.capsule(荚膜或大荚膜):粘液状物质具有一定外形,相对稳定地附着在细胞壁外,厚度:>0.2µm3.flagella(鞭毛):从细胞质膜和细胞壁伸出细胞外面的蛋白质组成的丝状体结构,使细菌具有运动性。
4.菌落:将单个细菌细胞接种到适宜的固体培养基中,在适宜的条件下培养,细菌迅速的生长繁殖,经过一段时间后,可在培养基的里面或表面聚集形成一个肉眼可见的、具有一定形态的子细胞群体,称为菌落。
5.假菌丝:酵母菌进行芽殖后,长大的子细胞不与母细胞立即分离,并继续出芽生殖,细胞成串排列,这种菌丝状的细胞串就称为假菌丝。
6.出芽痕和诞生痕:酵母出芽繁殖时,子细胞与母细胞分离,在子、母细胞壁上都会留下痕迹。
在母细胞的细胞壁上出芽并与子细胞分开的位点称出芽痕,子细胞细胞壁上的位点称诞生痕。
7.病毒:是一类不具细胞结构,具有遗传、复制等生命特征的微生物。
8.病毒粒子:完整的、具有感染性的病毒颗粒。
又称病毒体。
9.烈性噬菌体:指感染宿主细胞后,能够使宿主细胞裂解的噬菌体。
10.温和噬菌体(或溶原性噬菌体):噬菌体感染细胞后,将其核酸整合到宿主的核DNA上,并且可以随宿主DNA的复制而进行同步复制,在一般情况下,不引起寄主细胞裂解的噬菌体。
11.噬菌斑:将少量噬菌体与大量敏感菌混合培养在营养琼脂中,在平板表面布满宿主细胞的菌苔上,可以用肉眼看到一个个透明的不长菌的小圆斑,称为噬菌斑。
12.原噬菌体(或前噬菌体):即整合在宿主核DNA上的噬菌体的核酸。
13.溶原性噬菌体:同1014.营养(或营养作用,nutrition):微生物从外界摄取对其生命所必须的能量和营养物质,以满足正常生长和繁殖需要的一种最基本的生理功能称为营养。
15.碳源(carbon source):凡是提供微生物营养所需的碳元素(碳架)的营养源,称为碳源。
(完整版)微生物学名词解释微生物学名词解释【注:未收录英文较易推测且意义简单的名词,如carbon source,synthetic medium等】原核微生物1、microorganism微生物:一切肉眼看不见或看不清的微小生物的总称(不是一个分类阶元),它们都是一些个体微小、构造简单的低等生物,包括原核、真核和非细胞三大类。
2、bacteria细菌:一类细胞细短、结构简单、胞壁坚韧、多以二分裂方式繁殖和水生性较强的原核生物。
3、质壁空间:一般指位于G-细菌细胞壁外膜与细胞膜之间的狭窄空间,呈胶状,内含各种周质蛋白,包括各种酶类和受体蛋白等。
4、(endo)spore芽孢:某些细菌在生长发育后期,在细胞内形成一个圆形或椭圆形、厚壁、含水量极低、抗逆性极强的休眠体。
5、Spore coat芽孢衣:芽孢外层的一种主要结构,主要含有疏水性角蛋白,抗酶解、抗药物,多价阳离子难通过,使芽孢具有耐热性。
6、Parasporal crystal伴孢晶体:少数芽孢杆菌在形成芽孢的同时会在芽孢旁形成一颗菱形、方形或不规则的碱溶性蛋白晶体,即为伴孢晶体。
7、carboxysome羧酶体:存在于一些自养细菌细胞内的多角形内含物,内含1,5-二磷酸核酮糖羧化酶,在CO2固定中起着关键作用。
8、L-form bacteriaL-型细菌:在实验室或宿主体内通过自发突变而形成的遗传性稳定的细胞壁缺损菌株。
9、fimbria菌毛:一类长在细菌体表的纤细、中空、短直、数量较多的蛋白质附属物,具有使菌体附着于物体表面的功能。
10、flagella鞭毛:生长在某些细菌表面的长丝状、波曲的蛋白质附属物,其数目为一至数十条,具有运动能力。
11、sexpilus(pl. pili)性毛:构造与成分与菌毛相同,但比菌毛长,每个细胞一至少数几根,一般见于G-雄性菌株,具有传递遗传物质的功能。
12、mesosome间体:细菌细胞中一种由细胞质膜内褶而形成的囊状结构,其中充满着层状或管状的泡囊,多见于G+细菌。
第一章绪论Microorganism 微生物: 是广泛存在于自然界的一大群形体微小、结构简单、肉眼直接看不见,必须借助光学显微镜或电子显微镜放大数百倍、数千倍,甚至数万倍才能观察到的微小生物。
P1第一章细菌的形态与结构peptidoglycan 肽聚糖:又称粘肽(mucopeptide)、胞壁质(murein)或粘质复合物(mucocomplex),是细菌细胞壁中的共有成分,支持细胞壁的机械强度。
在G+菌,其肽聚糖由聚糖支架(为N-乙酰葡糖胺和N-乙酰胞壁酸两种氨基糖交替间隔排列,经β-1,4糖苷键连接形成的多糖支架),四肽侧链和五肽交联桥组成;而在G-菌,则仅由聚糖支架和四肽侧链两部分构成。
P11teichoic acid 磷壁酸:是由核糖醇或甘油残基经磷酸二酯键相互连接而成的多聚物,是革兰阳性细菌细胞壁的特有组分,与G+菌的抗原性及致病性关系密切。
根据结合位置的不同,磷壁酸可分为膜磷壁酸和壁磷壁酸两大类。
☆lipopolysaccharide, LPS 脂多糖:也称内毒素(endotoxin),位于革兰阴性菌细胞壁外膜的最外层,由脂质A、核心多糖、特异性侧链三部分组成。
其中脂质A为内毒素的毒性部分和生物活性组分,无种属特异性。
P13☆L-form of bacteria L型细菌:即细胞壁缺陷型细菌,指细菌细胞壁的肽聚糖结构因理化或生物因素的直接破坏或合成受到抑制而形成的胞壁受损的细菌,在高渗环境下仍可存活进而成为胞壁缺陷型细菌。
因英国李斯德(Litser)研究所首先发现而得名。
P14 ☆Protoplast原生质体:指G+细菌的细胞壁肽聚糖因理化或生物因素的直接破坏或合成受抑形成的细胞壁缺陷型细菌。
原生质体多呈椭圆形,其原生质仅由一层细胞膜包裹,对渗透压较为敏感。
P14periplasmic space 周浆间隙:位于G-菌细胞膜与外膜脂质双层之间的空隙,常含有多种酶类物质,在细菌获取营养,解除有害物质毒性等方面有重要作用。
微生物英文名词解释1.Mycoplasma:The mycoplasma are a group of the smallest organisms withoutcell wall that can be free-living in nature, can pass through bacterial filter and also grow on laboratory media.2.Chlamydia:Chlamydia are small Gram-negative bacteria which are obligateintercellular parasites like virus, but differ from them in that they have both RNA and DNA, ribosome, cell wall, and divided by binary fission.3.L forms of bacteria:In osmotically protective media, removal of the bacterialwall with lysozyme or penicillin liberate protoplasts from Gram-positive cells and spheroplasts from Gram-negative cells. If such wall-defective cells are able to grow and divide, they are called L forms. L forms are difficult to cultivate. They require a special media. Some L form can revert tothe normal bacillary form. L form in the host may produce chronic infection that are relatively resistant to antibiotic treatment.4.Capsule:Many bacteria synthesize large amounts of extracellular polymer whengrowing in their natural environments. When the polymer forms a condensed, well-defined layer closely surrounding the cell, it is called the capsule. With one known exception (the polypeptide capsule), the polymer is polysaccharide.5.Pyrogen:This is a fever-producing substance synthesized by bacteria. In fact, itis the lipopolysaccharide of Gram-negative bacteria. For the injectable medicament, it is especially important to avoid the contamination of pyrogen in the course of pharmic production.6.Exotoxin:Exotoxins are proteins produced inside Gram-positive bacteria cellsand secreted into the environment. These toxins are some of the strongest poisons known to man and cause violent reactions in host organisms.7.Endotoxin:Endotoxins are made up of lipids and carbohydrates associated withthe outer membrane of gram-negative bacteria. These toxins usually produce fever, weakness, and capillary damage.8.Disinfection: Reduce or eliminate pathogens病原体in or on inanimate无生命的objects to a safe level, which are no longer health hazard危险.9.Sterilization: A physical or chemical process that completely destroys orremoves all microbial life,including bacteria spore and viruses.10.Antisepsis: Use chemical agents to inhibit or destroy the growth ofmicroorganisms on skin or other living tissue.11.Plasmids:Plasmids are small genetic elements that replicate independently of thebacterial chromosome. Most plasmids are circular, double-stranded DNA molecules varying from 1,500 to 400,000 base pairs. Like the bacterial chromosomal DNA, they can autonomously replicate and as such are referred to as replicons.12.Transformation:It is the process by which bacteria take up fragments of nakedDNA and incorporate them into their genomes. During transformation, DNAfragments from a dead degraded bacterium bind to DNA binding proteins on the surface of a competent recipient bacterium.13.Conjugation:Conjugation is the transfer of DNA directly from a living donorbacterium to a recipient bacterium during the mating of the bacteria. A sex pilus produced by the donor bacterium (or male) binds to the recipient (or female). The sex pilus then retracts, bringing the two bacteria in contact and the transferred DNA passes through the sex pilus.14.Transduction:Transduction is mediated by a bacteriophage, which pick upfragments of DNA and package them into bacteriophage particles. The DNA is delivered to infected cells and becomes incorporated into the bacterial genomes.15.Normal flora:The various bacteria and fungi that are permanent residents ofcertain body sites, especially the skin, oropharynx, colon, and vagina.16.Dysbacteriosis:If flora disequilibrium occurs, for example, when the residentflora is disturbed, some little significant microorganisms may colonize, proliferate and produce diseases, which are called dysbacteriosis. Dysbacteriosis mainly result from long term and large bacteriosis mainly result from long term and large bacteriosis mainly result from long term and large doses antibiotics taken. Antibiotics can suppress the drug-susceptible components of fecal flora.Soon thereafter the counts of fecal flora rise again to normal or higher than normal levels, principally of organisms selected out because of relative resistance to the drugs employed. The drug susceptible microorganisms are replaced by drug-resistant ones and cause correlative disease, microbial selection and substitution.17.Toxemia:Bacteria multiply at invading location and do not enter blood stream,but the exotoxins enter blood and cause corresponding toxic symptoms.18.Endotoxemia:Gram-negative bacteria multiply at location or in blood stream,release a lot of amount endotoxin released from bacterial cell rupture.19.Protein A :Protein A is a cell wall component of many S. aureus strains thatbinds to the Fc portion of IgG molecules except IgG3. The Fab portion of IgG bound to protein A is free to combine with a specific antigen. Protein A has become an important reagent in immunology and diagnostic laboratory technology; for example, protein A with attached IgG molecules directed againsta specific bacterial antigen will agglutinate bacteria that have that antigen(“coagglutination”). Protein A probably contributes to the virulence of S. aureus by interfering with opsonization.20.Elementary body(EB):Elementary body(EB)are small round cells about0.2~0.4μm with an electron-dense nucleoid. They possess a cell wall. They areextracellular form and the environmentally stable infectious particle ,and metabolically inactive. The EB have a high affinity for host epithelial cells and rapidly enter them,creating a protected membrane-bound environment around the chlamydiae,and the Elementary body is reorganized into a larger one called metabolically active Reticulate body(RB).21.Reticulate body(RB):The intracellular large form known as the reticulate bodyMeasuring about 0.5~1μm and devoid of an electron-dense nucleoid. Itspresence will reflect the stage of replication. Within the membrane-bound vacuole,the elementary grows in size and divides into repeatedly by binary fission. Eventually,the entire vacuole becomes filled with elementary bodies derived from reticulate bodies to form a cytoplasmic inclusion. The newly formed elementary bodies may be liberated from the host cell to infect new cells. The developmental cycle takes 24-48 hours.22.nucleocapsid:The simplest of virus particles consists of a protein coat (capsid)which surrounds a strand of nucleic acid and are thus called naked viruses or nucleocapsid.23.Abortive Infections:Not all infections lead to new progeny virus. Productiveinfections occur in permissive cells and result in the production of infectious virus. Abortive infections fail to produce infectious progeny, either because the cell may be nonpermissive and unable to support the expression of all viral genes or because the infecting virus may be defective, lacking some functional viral gene.24.defective virus:A defective virus is one that lacks one or more functional genesrequired for viral replication. Defective viruses require helper activity from another virus for some step in replication or maturation. One type of defective virus lacks a portion of its genome (i.e., deletion mutant). The extent of loss by deletion may vary from a short base sequence to a large amount of the genome.Deletion mutants may arise spontaneously or may be constructed in the laboratory using biochemical techniques.25.Interferons (IFN s):Interferons (IFN s)are proteins made and released by hostcells in response to the presence of pathogens such as viruses, bacteria, parasites or tumor cells. They allow for communication between cells to trigger the protective defences of the immune system that eradicate pathogens or tumors. 26.Antigenic drift: Antigenic drift is constantly occurring in both influenza A andinfluenza B viruses. The HA and/or NA of the new strain are sufficiently different to evade (at least partially) the pre-existing human immunity. This leads to the seasonal epidemics.27.Antigenic shift:Antigenic shift occurs only in influenza A virus. It describes theemergence of an entirely new virus sub type. When this new sub type emerges, it causes a pandemic because there is no pre-existing immunity in humans.。
1.Plasmid(质粒):是细菌染色体外的遗传物质,存在于细胞质中,为闭合的环状双股DNA,带有遗传信息,控制细菌的某些特定的遗传性状.2.Colony(菌落):单个细菌经一定时间培养后形成的一个肉眼可见的细菌集团称为菌落.3.Prophage(前噬菌体):在溶原状态下,整合在细菌染色体上的噬菌体基因组称为前噬菌体.4.Conjugation(接合):细菌通过性菌毛相互连接沟通,将遗传物质(主要是质粒DNA)从供体菌转移给受体菌,使后者获得前者的部分遗传性状的过程.5.Lysongenic conversion(溶原性转变):温和噬菌体的DNA作为一种外源性基因与细菌染色体通过溶原性整合而重组,使细菌的遗传结构发生改变而导致细菌性状的改变.6.Bacteremia(菌血症):在局部生长繁殖的病原菌一过性或间歇性进入血流,但未在血中繁殖,全身中毒症状轻.7.Endotoxemia(内毒素血症):革兰阴性菌侵入血流,并在血中大量繁殖.死亡分解释放出大量的内毒素或细菌在局部繁殖后,分解释放大量的内毒素进入血液,引起如发热,DIC,休克等轻重不同的临床症状.8.Toxoid(类毒素):外毒素可用人工方法处理脱毒,但保留抗原性,用于人工主动免疫的制剂称为类毒素.9.Tuberculin test(结核菌素试验):用旧结核菌素/纯蛋白衍生物为试剂做皮肤实验,通过观察注射局部是否出现红肿,硬节等迟发型超敏反应,判断机体有无结核分枝杆菌感染和对该菌的免疫力,感染过结核分枝杆菌或接种过卡介苗者一般都出现阳性反应.10.Inapparent infection(隐性感染):当机体抗感染免疫力较强或入侵细菌数量不多,毒力较弱,感染后对机体损害较轻,不出现或出现不明显的临床症状,又称亚临床感染.11.SPA(葡萄球菌A蛋白):是葡萄球菌细胞壁的一种表面蛋白,能与人及某些哺乳类动物的IgG分子Fc段发生非特异性结合,与吞噬细胞的Fc受体争夺Fc段,从而降低了抗体的调理吞噬作用,起到了协助细菌抗吞噬的作用.12.Nucleocapsid(核衣壳):病毒核心与衣壳共称为核衣壳,是病毒所必需的基本结构,病毒核酸构成核心,包围在核心周围的蛋白质构成衣壳.13.Abortive infection(顿挫感染/流产性感染):是指有些宿主细胞不能全部提供病毒复制所需的必要因子,致使所复制的病毒为不完整的、无感染性的病毒颗粒或亚颗粒.这种感染过程称称流产性感染.14.Horizontal transmission(水平传播):病毒在人群中不同个体之间的传播称为水平传播.15.Slow viral infection(慢发病毒感染):病毒或致病因子感染后,经过很长的潜伏期,有的可达数年或数十年之久,以后出现慢性进行性疾病,直至死亡.16.Viral neutralizing antibody(病毒中和抗体):指针对病毒某些表面抗原的抗体,此抗体可与细胞外游离的病毒结合后阻碍病毒吸附,并能消除或减弱病毒感染性,成为中和抗体.17.Antigenic shift(抗原转换):指甲型流感病毒每隔十数年发生抗原性大变异(或质变)而产生新的亚型.抗原转变可分为大组变异(HA和NA均变异)和亚型变异(仅HA变异.而NA未变或小变异)两种.18.HBeAg(乙肝病毒e抗原):由HBV的前C和C基因编码的一种可溶性蛋白,可存在于感染者血液中,其消长与病毒体及DNA聚合酶的消长基本一致,故HBeAg可作为体内HBV复制及血液具有强感染性的一个指标.19.HIV(人体免疫缺陷病毒):属逆转录病毒科,核心为单正链RNA二聚体,含有逆转录酶,是获得性免疫缺陷综合症的病原体,主要通过性接触,血液和垂直传播三种方式传播.20.Prion(朊病毒):又称传染性蛋白粒子.其主要成分是一种蛋白酶抗性蛋白,对各种理化作用的抵抗力强,不含核酸.它具有传染性,潜伏期长,在人和动物中引起以海绵状脑病为特征的致死性中枢神经系统的慢性退行性疾病.21.Widal test(肥达氏试验):系用已知的伤寒杆菌O、H抗原和甲、乙型副伤寒杆菌的H抗原,与不同稀释度的待检血清作定量凝集试验,根据抗体的含量和动态变化以辅助临床诊断伤寒、副伤寒的一种血清学试验.22.IMViC test(IMViC试验):吲哚试验(I),甲基红试验(M)、VP试验(V)、柠檬酸试验(C)四种试验常用于肠道杆菌的鉴别,合称IMViC试验.23.TAT(破伤风抗毒素):能中和破伤风毒素的特异性抗体.主要用于破伤风感染的治疗和紧急预防.。
◎第1章1.微生物(mi cr oor g an is m):通常描述一切不借助显微镜用肉眼看不见的微小生物。
这类微生物包括病毒、细菌、古菌、真菌、原生生物和某些藻类。
2.微生物学(m icr o bi ol og y):指大量的、极其多样的、不借助显微镜看不见的微小生物类群的总称。
微生物通常包括病毒、亚病毒(类病毒、拟病毒、朊病毒)、具原核细胞结构的真细菌、古生菌以及具真核细胞结构的真菌(酵母、霉菌)、原生动物和单细胞藻类。
3.分子微生物学(mo le cu la r mi cro b io lo gy):在分子水平上研究微生物生命活动规律的科学。
4.细胞微生物学(ce ll ul ar mi cr ob i ol og y):重点研究微生物与寄主细胞相互关系的科学。
5.微生物基因组学(mi cr ob ic g eno m ic s):研究微生物基因组的分子结构、信息含量及其编码的基因产物的科学。
6.自生说(s po nta n eo us ge ne ra ti on):一个古老的学说,认为一切生命有机体都能够从无生命的物质自然发生。
7.安东·列文虎克(An to ny va n Le e uw en ho ek,1632-1723):荷兰商人,他是真正看见并描述微生物的第一人,他利用自制放大倍数为50~300倍的显微镜发现了微生物世界(当时称之为微小动物),首次揭示了一个崭新的生物世界――微生物界。
8.路易斯·巴斯德(Lo ui s Pa st eur,1822-1895):法国人,原为化学家,后来转向微生物学研究领域,为微生物学的建立和发展作出了卓越的贡献,成为微生物学的奠基人。
主要贡献:用曲颈瓶实验彻底否定了“自生说”,从此建立了病原学说,推动了微生物学的发展;研究了鸡霍乱,发现将病原菌减毒可诱发免疫性,以预防鸡霍乱病,其后他又研究了牛、羊炭疽病和狂犬病,并首次制成狂犬疫苗,证实其免疫学说,为人类防病、治病作出了重大贡献;分离到了许多引起发酵的微生物,并证实乙醇发酵是由酵母菌引起的,也发现乳酸发酵、醋酸发酵和丁酸发酵是由不同细菌所引起的,为进一步研究微生物的生理生化和工业微生物学奠定了基础。
微⽣物学名词解释1、微⽣物学(Microbiology):是⼀门在细胞、分⼦或群体⽔平上研究微⽣物的形态构造、⽣理代谢、遗传变异、⽣态分布和分类进化等⽣命活动基本规律,并将其应⽤于⼯、农、以及环境保护等实践领域的科学。
2、灭菌(sterilization):采⽤强烈的理化因素使任何物体内外部的⼀切微⽣物永远丧失其⽣长繁殖能⼒的措施。
3、消毒(disinfection):采⽤较温和的理化因素仅杀死物体表⾯或内部⼀部分对⼈体有害的病原菌,⽽对被消毒的物体基本⽆害的措施。
4、菌落(colony):单个(或聚集在⼀起的⼀团)微⽣物在适宜的固体培养基表⾯或内部⽣长、繁殖到⼀定程度可以形成⾁眼可见的、有⼀定形态结构的⼦细胞⽣长群体。
5、菌苔(lawn):众多菌落连成⼀⽚形成。
6、平板(plate):被⽤于获得微⽣物纯培养的最常⽤的固体培养基形式,是冷却凝固后的固体培养基在⽆菌培养⽫中形成的培养基固体平⾯。
7、糖被(glycocalyx):包被在某些细菌细胞壁外的⼀层厚度不定的胶状物质。
根据其有⽆固定层,层的厚度⼜可以分为荚膜(capsule)微荚膜(microcapsule)、粘液层和菌胶团。
8、趋化性(Chemotaxis):单细胞或多细胞⽣物在它们所处的环境中的某些化学物质的指令下,进⾏定向运动的特征。
9、肽聚糖(peptidoglycan):是真细菌细胞壁中特有的成分,由肽聚糖单体聚合⽽成。
10、原⽣质体(protoplast):⼈为条件下⽤溶菌酶除尽原有的细胞壁或者⽤青霉素抑制新⽣细胞壁合成所得到的仅有细胞膜包裹着的圆球状渗透敏感细胞。
11、L型细菌(L-form of bacteria):实验室诱发或者在宿主体内形成的⽆细胞壁的细菌。
12、芽孢(endospore):某些种类的细菌在⼀定的时期,其细胞内产⽣特殊休眠结构。
13、真菌(fungi):是⼀类单细胞或者能形成丝状分枝的营养体,有细胞壁和细胞核,不含有叶绿素和其他光合⾊素,有性⽣殖和⽆性⽣殖产⽣孢⼦的⽣物群。
1.微生物:(Microbiology)是一切肉眼看不见或看不清的微小生物的总称。
2.芽胞:细菌在生长发育后期,在细胞内形成一个(椭)圆形厚壁,含水量低,抗逆性强的休眠构造。
(P23)3.碳素养料:凡是能为微生物生长提供碳素来源的物质4.氮素养料:凡是能为微生物生长提供碳素来源的物质6.选择培养基:是指根据某种微生物的生长的特殊要求或对某种化学、物理因素的抗性而设计的培养基。
7.鉴别培养基;是指培养基反应中加入能与某菌的代谢产物发生显色反应的化学试剂,从而用肉眼就能识别所研究的细菌。
8.光合磷酸化:是由光照引起的电子传递作用与磷酸化作用相偶联而生成ATP的过程,即将光能转化为化学能的过程。
P489.发酵作用:不需要外源电子受体的基质能量代谢。
发酵过程中,微生物将有机物氧化释放的氢直接交给底物本身未完全氧化的某种中间产物,同时释放能量并产生各种代谢产物。
10.有氧呼吸:有氧呼吸即呼吸作用,是指微生物氧化底物时以分子氧作为最终电子受体的氧化作用。
11.无氧呼吸:无氧呼吸是指在无氧条件下,通过酶的作用,细胞把糖类等有机物分解不彻底的氧化产物,同时释放少量能量的过程(课本P51)12.酒精发酵:酵母菌在无氧条件下将丙酮酸转化成乙醇的过程。
13.同型发酵14.异型发酵:异型发酵是指一些乳酸菌在发酵过程中使发酵液中大约50%的糖转化为乳酸,另外的糖转变为其他有机酸、醇、二氧化碳、氢等。
15.丁酸发酵:这是由专性厌氧的梭状芽孢杆菌所进行的一种发酵,因产物中有丁酸,故称为丁酸发酵。
16.次生代谢;是微生物合成一些与其本身的生命活动没有明确功能的物质过程。
其产物包括糖苷类、多肽类、萜烯类、芳香类等化合物。
(P64)17.质粒;许多细菌细胞中还有染色体以外的DNA分子,一般以不同大小的环状双螺旋状态存在,称为质粒。
(P21)18.野生型(wild type);从自然界分离获得的茵株称为野生型。
(P89)19.突变体(mutant);细胞中DNA碱基和碱基序列的任何改变称为突变,如果变化了的碱基在复制后稳定地存在于子代中,则成为突变型。
名词解释:1、微生物(microbe ,microorganism) 是指肉眼看不见或看不清楚的微小生物的总称。
微生物是所有形体微小、单细胞或结构较为简单的多细胞生物、甚至没有细胞结构的生物的通称。
2、细菌:是一类细胞细而短(细胞直径约0.5um,长度约0.5~5um)、结构简单、细胞壁坚韧、以二等分裂方式繁殖和水生性较强的原核微生物。
3、磷壁酸teichoic acid(垣酸):占壁干重40~50%。
是以磷酸多元醇分子的重复结构单位为主链(骨架)的阴离子多聚物。
(在多数情况下,磷壁酸分子中的磷酸多元醇是磷酸甘油,或磷酸核糖醇,因此,根据主链组成不同可以将磷壁酸分为两大类:磷酸甘油型磷壁酸和核糖醇型磷壁酸。
)4、脂多糖:是革兰氏阴性细菌细胞壁中的一种成分,脂多糖对宿主是有毒性的。
脂多糖只有当细菌死亡溶解或用人工方法破坏菌细胞后才释放出来,所以叫做内毒素。
5、芽殖:在母细胞表面(尤其在其一端)先形成一个小突起,待长大到与母细胞相仿后再相互分离并独立生活的一种繁殖方式。
6、缺壁细菌:在溶菌酶或青霉素作用下产生细胞壁缺陷或无细胞壁的细菌。
7、L型细菌(L-form of bacteria):L型细菌专指那些在实验室或宿主体内通过自发突变而形成的遗传性稳定的细胞壁缺陷菌株。
8、放线菌Actinomycetes:是一类具有丝状分枝的单细胞,主要以外生孢子的形式繁殖,革兰氏阳性,与细菌同属原核微生物。
放线菌菌落中的菌丝常从一个中心向四周辐射状呈放射状生长,并因此而得名。
放线菌有特殊的土霉味。
9、菌丝(hyphae):具有G+菌细胞壁丝状细胞,没有间隔,为多核体。
可分为基内菌丝、气生菌丝和孢子丝。
成团的菌丝为菌丝体。
10、基内菌丝(substrate hyphae): 培养基内匍匐生长的菌丝,无隔,约0.2-0.8μm。
通常会产生水溶性或脂溶性色素。
功能:吸收营养,所以又称营养菌丝。
11、气生菌丝(aerial hyphae):由营养菌丝长出培养基外,伸向空间的菌丝。
微生物(microbe,microorganism):微生物是指大量的、极其多样的、只有借助显微镜才能看得见的微小生物类群的总称。
微生物学的基本内容:①微生物细胞的结构和功能,研究细胞的构建及其能量、物质、信息的运转;②微生物的进化和多样性,研究微生物的种类,它们之间的相似性和区别,以及微生物的起源;③生态学规律,研究不同微生物之间以及它们同环境之间的相互作用;④微生物同人类的关系。
微生物的特点:1体积小,比表面积大;2吸收多,转化快3生长旺,繁殖快;4适应强,易变异;5分布广,种类多;群体(population):具有相似特性和生活在一定空间内的同种个体群,是组成群落的基本组分。
群落(community):在一定区域或一定生态环境内,各种生物群体构成的一个生态学结构单位,群落中各生物群体之间存在各种相互作用。
试述细菌革兰氏染色的原理和结果:革兰氏染色是1884年由丹麦人革兰氏(christan gran)发明的,其染色要点:1、先用结晶紫染色,菌体呈紫色;2、再加碘液煤染,菌体呈紫色,3、然后用乙醇脱色,革兰氏阳性菌呈紫色,革兰氏阴性菌无色;4、最后用沙黄或香红复染,革兰氏阳性菌呈紫色,阴性菌呈红色。
革兰氏染色结果与细胞壁组成有关,因为革兰氏阳性细胞壁较厚,肽聚糖含量较高,网络结构紧密,含脂量又低,当它被酒精脱色时,引起了细胞壁肽聚糖网络状结构的孔径缩小,从而阻止了不溶性结晶紫-碘复合物的逸出,故菌体呈紫色;可是革兰氏阴性菌的细胞壁肽聚糖层较薄,含量低,而脂类含量高,当酒精脱色时,脂类物质溶解,细胞壁透性增大,结晶紫-碘复合物也随之杯抽提出来,故革兰氏阴性菌成复染液的红色。
细胞膜功能:1.维持渗透压梯度和溶质的转移;半渗透膜:具有选择性的渗透作用,能阻止高分子通过,并选择性地逆浓度梯度吸收某些低分子进入细胞。
膜有极性:膜上有各种与渗透有关的酶,还可使两种结构相类似的糖进入细胞的比例不同,吸收某些分子,排出某些分子;2.参与细胞壁、糖被合成;细胞质膜上有合成细胞壁和形成横隔膜组分的酶。
/kech/wswx/cn/test/shiti.htm华中农业大学微生物试题集(仅参考)*不做重点*Section 1 Microorganisms and Microbiology1Archaea :a group of phylogenetically related prokaryotes distinct from Bacteria2Bacteria :a group of phylogenetically related prokaryotes distinct from Archaea 3 Chromosome:a genetic element carrying genes essential to cell function4DNA :deoxyribonucleic acid, the hereditary material of cells and some viruses 5 Enrichment culture:a method for isolating microorganisms from nature using specific culture media and incubation conditions6 Enzymes :protein catalysts that function to speed up chemical reactions7 Eukaryote:a cell possessing a membrane-enclosed nucleus and usually other organelles8Genetics :heredity and variation of living organisms9Microorganism :a microscopic organism consisting of a single cell or cell cluster, including the viruses10 Plasmid:a small genetic element that exists separately from the chromosome 11Pure culture :a culture containing a single kind of microorganism12Spontaneous generation :the hypothesis that living organisms can originate from nonliving matter13Aseptic :technique methods for maintaining sterile culture media and other sterile objects free from microbial contamination during manipulations14Cell :the fundamental unit of living matter15Cytoplasm :the fluid portion of a cell, bounded by the cell membrane but excluding the nucleus (if present)16Ecology :the study of organisms in their natural environments17 Entropy:a measure of the degree of disorder in a system; entropy always increases in a closed system18Eukarya :all eukaryotic organisms19Evolution :change in a line of descent over time leading to the production of new species or varieties within a species20Metabolism :all biochemical reactions in a cell21 Pathogen:a disease-causing microorganism22 Prokaryote:a cell lacking a nucleus and other organelles23 RNA:ribonucleic acid, involved in protein synthesis as messenger RNA, transfer RNA, and ribosomal RNA24 Sterile:absence of all living organisms and virusesSection 2 Cell Biology1Antigen-Binding Cassette transporter :a membrane transport system consisting of three proteins, one hydrolyzes ATP as an energy source to drive the transport event, one binds the substrate on the outside of the cell, and one functions as the transport channel through the membrane2Chloroplast :the chlorophyll-containing photosynthetic organelle of eukaryotic photosynthetic organisms3 Cytoplasmic:membrane the permeability barrier of the cell, separating the cytoplasm from the environment4 Eukaryote :a cell containing a membrane-enclosed nucleus and usually other organelles5Gas vesicles :gas-filled cytoplasmic structures bounded by protein and coriferring buoyancy on cells6 Gram-positive:a prokaryotic cell whose cell wall consists chiefly of peptidoglycan and lacks the outer membrane of gram-negative cells7Lipopolysaccharide (LPS):lipid in combination with polysaccharide and protein forming the major portion of the cell wall in gram-negative Bacteria8 Mitochondrion (mitochondria):an organelle found in most eukaryotic cells in which respiration and energy generation occurs9Nucleus:a membraned-enclosed structure in cells of Eukarya that contains the genetic material, arranged in chromosomes10 Flagellum:Peptidoglycan :a polysaccharide composed of alternating repeats of acetylglucosamine and acetylmuramic acid with the latter in adjacent layers cross-linked by short peptides11 Peritrichous:in reference to flagellation pattern; flagella located in many places around the surface of the cell Phototaxis movement of an organism toward light12 Prokaryote:a cell that lacks a membrane-enclosed nucleus and that usually has a single circular DNA molecule as its chromosome13 Ribosome :small particles composed of RNAs and proteins that function in protein synthesis14Chemotaxis :movement of an organism toward (positive) or away from (negative) a chemical gradient15Chromosome :a DNA molecule, usually circular in prokaryotes and linear in eukaryotes, carrying genes essential to cellular function16Endospore:a highly heat-resistant, thick-walled, differentiated cell produced by certain gram-positive Bacteria17 Flagellum :a long, thin cellular appendage capable of rotation in prokary otic cells and responsible for swim-ming motility18 Gram-negative:a prokaryotic cell whose cell wall contains relatively little peptidoglycan but contains an outer membrane composed of lipopolysaccharide, lipoprotein, and other complex macromolecules19 Group translocation:an energy-dependent transport process in which the substance transported is chemi-cally modified during the transport process20 Magnetosomes:particles of magnetite (Fe3O4) organized into nonunit membrane-enclosed structures in the cytoplasm of magnetotactic Bacteria21 Nucleoid:an aggregated state of the circular chromosome of prokaryotic cells22 Organelle:a unit membrane-enclosed structure found in the cytoplasm of eukaryotic cells23Periplasm :a gellike region between the outer surface of the cytoplasmic membrane and the inner surface of the lipopolysaccharide layer of gram-negative Bacteria24 Poly-P-hydroxybutyrate (PHB):a common storage material of prokaryotic cells consisting of a polymer of P-hydroxybutyrate or another 3-alkanoic acid25 Protoplast :an osmotically protected cell whose cell wall has been removedSection3 Prokaryotic Microorganisms1 Acid fastness:a property of Mycobacterium species in which cells stained with the dye basic fuchsin resist decolorization with acidic alcohol2Chemolithotrophs :organisms able to oxidize inorganic compounds as energy sources3 Consortia:two or more-membered association of prokaryotes, usually living in an intimate symbiotic fashion4Enteric bacteria :a large group of gram-negative rod-shaped Bacteria characterized by a facultatively aerobic metabolism5 Heliobacteria:anoxygenic phototrophs containing bacteriochlorophyll g6Heterocyst :a differentiated cyanobacterial cell that carries out nitrogen fixation but not oxygenic photosynthesis7Homofermentative :in reference to lactic acid bacteria, producing only lactic acid as a fermentation product8 Methylotroph :an organism capable of oxidizing organic compounds that do not contain carbon-carbon bonds; if able to oxidize CH4, also a methanotroph9 Purple nonsulfur bacteria:a group of phototrophic prokaryotes containing bacteriochlorophylls a or b that grow best as photoheterotrophs and have a relatively low tolerance for H2S10 Prostheca :an extrusion of cytoplasm often forming a distinct appendage, bounded by the cell wall11 Pseudomonad:member of the genus Pseudomonas, a large group ofgram-negative, obligately respiratory (never fermentative) Bacteria12 Spirochete :a slender, tightly coiled gram-negative prokaryote characterized by possession of axial filaments used for motility13 Sulfate-reducing bacteria :a large group of anaerobic Bacteria that respire anaerobically with SO42- as electron acceptor, producing H2S14 Carboxysomes :polyhedral cellular inclusions of crystalline ribulose bisphosphate carboxylase (RubisCO), the key enzyme of the Calvin cycle15Chlorosomes :cigar-shaped structures bounded by a nonunit membrane and containing the light-harvesting bacteriochlorophyll (c, d, or e) in green bacteria and Chloroflexus16 Cyanobacteria :prokaryotic oxygenic phototrophs that contain chlorophyll a and phycobilins but not chlorophyll b17 Green bacteria:anoxygenic phototrophs containing chlorosomes and bacteriochlorophyll c, cs, d, or e as light-harvesting chlorophyll18 Hyperthermophile:an organism with a growth temperature optimum of greater than 80oC19Heterofermentative :in reference to lactic acid bacteria, capable of making more than one fermentation product20 Methanotroph :an organism capable of oxidizing methane (CH4)21 Nitrifying bacteria:chemolithotrophs capable of carrying out the transformation NH3?NO2- or NO2-?NO3-22 Prochlorophyte :a prokaryotic oxygenic phototroph that contains chlorophyllsa andb but lacks phycobilins23 Proteobacteria :a major lineage of Bacteria that contains a large number of gram-negative rods and cocci24Purple sulfur bacteria :a group of phototrophic prokaryotes containing bacteriochlorophylls a or b and characterized by the ability to oxidize H2S and store elemental sulfur inside the cells (or in the genus Ectothiorhodospira, outside the cell)25 Stickland reaction :fermentation of an amino acid pair in which one amino acid serves as an electron donor and a second serves as an electron acceptor Section4 Eukaryotic Microorganisms1Ameboid movement :a type of motility in which cytoplasmic streaming moves the organism forward2Chloroplast:the photosynthetic organelle of eukaryotic phototrophs3 Conidia:asexual spores of fungi4Flagellates :a group of protozoa characterized by motility driven by the whiplike action of one or more long,thin appendages called flagella5 Hydrogenosome:an organelle of endosymbiotic origin present in certain anaerobic eukaryotic microorganisms that functions to oxidize pyruvate to H2, CO2, and acetate, along with the production of one ATP6 Molds:filamentous fungi7Phagocytosis :a mechanism for ingesting particulate food in which a portion of the cell membrane surrounds the particle and brings it into the cell8 Slime molds:nonphototrophic eukaryotic microorganisms that lack cell walls and that aggregate to form fruiting structures (cellular slime molds) or masses of protoplasm (acellular slime molds)9 Yeasts:unicellular fungi10Chitin :a polymer of N-acetylglucosamine commonly found in the cell walls of algae and fungi11 Ciliates:a group of protozoa characterized by rapid motility driven by numerous short appendages called cilia12 Eukarya :all eukaryotic organisms13Fungi :nonphotosynthetic eukaryotic microorganisms that contain rigid cell walls14 Mitochondrion:the respiratory organelle of eukaryotic organisms15 Mushrooms :filamentous fungi that produce large, often edible structures called fruiting bodies16 Protozoa :unicellular eukaryotic microorganisms that lack cell walls17 Sporozoa :nonmotile parasitic protozoaSection 5 Viruses1Bacteriophage:a virus that infects prokaryotic cells2Lysogen:a bacterium containing a prophage3Oncogene:a gene whose expression causes formation of a tumor4Plus (positive)-strand nucleic acid :an RNA or DNA strand that has the same sense as the mRNA of a virus5Provirus (prophage):the genome of a temperate virus when it is replicating with, and usually integrated into,the host chromosome6Reverse transcription:the process of copying information found in RNA into DNA7Transformation :a process by which a normal cell becomes a cancer cell (but see alternative usage in Chapter 9)8Virulent virus:a virus that lyses or kills the host cell after infection; a nontemperate virus9Minus (negative)-strand nucleic acid:an RNA or DNA strand that has the opposite sense of (is complementary to) the mRNA of a virus10Plaque:a zone of lysis or cell inhibition caused by virus infection of a lawn of sensitive cells11Prion:an infectious agent whose extracellular form may contain no nucleic acid12 Retrovirus:a virus whose RNA genome has a DNA in-termediate as part of its replication cycle13Temperate virus :a virus whose genome is able to replicate along with that of its host and not cause cell death in a state called lysogeny14 Virion:the complete virus particle; the nucleic acid surrounded by a protein coat and in some cases other material15Virus:a genetic element containing either RNA or DNA that replicates in cells but is characterized by having an extracellular stateSection 6 Nutrition and Metabolism1Activation Energy energy the energy required to bring substrates to the reactive state2 Anabolism:the sum total of all biosynthetic reactions in the cell3 Catabolism:biochemical reactions leading to the production of usable energy (usually ATP) by the cell4 Chemoorganotroph :an organism that uses organic chemicals as energy sources (electron donors)5 Coenzyme :a small nonprotein molecule that participates in a catalytic reaction as part of an enzyme6 Culture medium :an aqueous solution of various nutrients suitable for the growth of microorganisms7 Electron acceptor:a substance that can accept electrons from some other substance, thereby becoming reduced in the process8 Enzyme:a protein that has the ability to speed up (catalyze) a specific chemical reaction9 Free energy :(G) energy available to do work10 Phototroph :an organism capable of using light as an energy source11 Reduction potential :the inherent tendency (measured in volts) of a compound to donate electrons12Siderophores :iron chelators that can bind iron present at very low concentrations13 Aerobe :a microorganism able to use O2 in respiration14 Autotroph :an organism capable of biosynthesizing all cell material from CO2 as the sole carbon source15 Chemolithotroph:an organism that uses inorganic chemicals as energy sources (electron donors)16 Citric acid cycle :a cyclical series of reactions resulting in the conversion of acetate to two CO217 Complex medium:a culture medium composed of digests of chemically undefined substances such as yeast and meat extracts18 Defined medium :a culture medium whose precise chemical composition is known19 Electron donor:a substance that can donate electrons to some electron acceptor, thereb y becoming oxidized in the process20 Fermentation:anaerobic catabolism in which an organic compound serves as both an electron donor and an electron acceptor and in which ATP is produced by sub-strate-level phosphorylation21 Oxidative phosphorylation:the production of ATP at the expense of a proton motive force formed by electron transport22Proton motive force :an energized state of the membrane resulting from the separation of charge and the elements of water (H+ versus OH?) across the membrane23Respiration :the process in which a compound is oxidized with O2 or an O2 substitute functioning as the terminal electron acceptor, usually accompanied by ATP production by oxidative phosphorylation24 Substrate-level phosphorylation :production of ATP by the direct transfer of a high energy phosphate molecule from a phosphorylated organic compound to ADPSection 7 Microbial Growth and Growth Control1 Acidophile:an organism that grows best at low pH2 Alkaliphile :an organism that grows best at high pH3Batch culture :a closed-system microbial culture of fixed volume4 Compatible solute:a molecule that is accumulated in the cytoplasm for adjustment of water activity but that does not inhibit biochemical processes5 Extremophile:an organism that grows optimally under one or more chemical or physical extremes, such as high or low temperature or pH6 Generation time:the time required for a population of microbial cells to double7 Halophile:a microorganism that requires NaCI for growth8 Lag phase:a period preceding the exponential growth phase when cells may be metabolizing but are not yet growing9 Mesophile:an organism that grows best at temperatures between 20 and 45oC10 Psychrophile:an organism with a growth temperature optimum of 15oC or lower and a maximum growth temperature below 20oC11 Stationary phase:the period immediately following exponential growth when the growth rate of the population falls to zero12Viable :capable of reproducing13 Aminoglycosides :a group of antibiotics, including streptomycin, containing amino sugars linked by glycosidic bonds14 Antibiotic resistance :the acquired ability of a microorganism to grow in the presence of an antibiotic to which the microorganism is usually sensitive15Antiseptic :antimicrobial agents that are sufficiently nontoxic to be applied on living tissues16β-Lactam antibiotics:a group of antibiotics, including penicillin, that contain the four-membered heterocyclic β-lactam ring17 Bacteriostatic:inhibits bacterial growth18 Decontamination:treatment that renders an object or inanimate surface safe to handle19 Disinfection:the process of eliminating nearly all pathogens, but not all microorganisms, from inanimate objects or surfaces20 Inhibition:the reduction of microbial growth because of a decrease in the number of organisms present or alterations in the microbial environment21 Lysis:loss of cellular integrity with release of cytoplasmic contents22 Semisynthetic penicillin :a natural penicillin that has been chemically altered23 Tetracycline:an antibiotic characterized by a four-mem-bered naphthacene ring24 Aerobe:an organism that can use O2 in respiration; some require O2 for growth25 Anaerobe :an organism that cannot use O2 in respiration and whose growth may be inhibited by O226 Chemostat:a device that allows for the continuous culture of microorganisms in which both growth rate and cell number can be controlled independently27 Exponential growth:growth of a microorganism where the cell number doubles within a fixed time period28 Facultative:with respect to O2, an organism that can grow in either its presence or absence29Growth :an increase in cell number30 Hyperthermophile:a microorganism that has a growth temperature optimum of 80oC or greater31 Lysis:loss of cellular integrity with release of cytoplasmic constituents32 Microaerophile:an aerobic organism that can grow only when oxygen tensions are reduced from that in air33Psychrotolerant :an organism capable of growth at low temperatures but whose growth temperature optimumis above 20oC34 Thermophile:an organism whose growth temperature optimum lies between 45 and 80oC35 Xerophile:an organism that is able to live, or that lives best, in very dry environments36Antibiotic :a chemical substance produced by a microorganism that kills or inhibits the growth of another microorganism37 Antimicrobial agent:a chemical that kills or inhibits the growth of microorganisms38 Autoclave:a sterilizer that destroys microorganisms with temperature and steam under pressure39 Bacteriocidal:kills bacteria40 Chemotherapeutic agent:an antimicrobial agent that can be used internally41 Disinfectant:an antimicrobial agent used only on inanimate objects42 Growth factor analog :a chemical agent that is related to and blocks the uptake of a growth factor43Interferons :host-specific antiviral proteins, produced by virus-infected cells, which prevents viral infection of neighboring cells44 Pasteurization:destruction of all disease-producing microorganisms or a reduction in the number of spoilage microorganisms45 Sterilization:the killing or removal of all living organisms and their viruses from a growth mediumSection 8 Microbial Genetics1Auxotroph :an organism that has developed a nutritional requirement through mutation2 Diploid:a eukaryotic cell or organism containing two sets of chromosomes3 Electroporation:the use of an electric pulse to induce cells to take up free DNA 4Genetic map:the arrangement of genes on a chromosome5 Genotype:the precise genetic makeup of an organism6 Mutagens:agents that cause mutation7 Mutation:an inheritable change in the base sequence of the genome of an organism8 Plasmid:an extrachromosomal genetic element that has no extracellular form9 Recombination:the process by which parts or all of the DNA molecules from two separate sources are exchanged or brought together into a single unit10Selection:placing organisms under conditions where the growth of those with a particular genotype will be favored11Transformation:transfer of bacterial genes involving free DNA (but see alternative usage in Chapter 8)12Conjugation:transfer of genes from one prokaryotic cell to another by a mechanism involving cell-to-cell contact and a plasmid13Transposon :a type of transposable element that carries genes in addition to those involved in transposition14Gametes:in eukaryotic organisms, the haploid germ cells resulting from meiosis15 Genome:the total complement of genes of a cell or a virus16 Haploid:a cell or organism that has only one set of chromosomes17Mutant:an organism whose genome carries a mutation18 Phenotype:the observable characteristics of an organism19 Point mutation:a mutation that involves one or only a very few base pairs20 Screening:any of a number of procedures that permit the sorting of organisms by phenotype or genotype, but allow the growth of those possible21 Transduction:transfer of host genes from one cell to another by a virus22Transposable element:a genetic element that has the ability to move (transpose) from one site on a chromosometo anotherSection 9 Microbial Ecology1Acid mine drainage :addic water containing H4SO4 derived from the microbial oxidation of iron sulfide minerals2Bacteroid:morphologically misshapen Rhizobium cells inside a leguminous plant root nodule; can fix N23Barophilic:an organism that grows best when placed under a pressure greater than 1 atm4 Biofilm:colonies of microbial cells encased in slime and attached to a surface5 Black smoker:an extremely hot (250-350oC) deep-sea hot spring emitting both hot water and various minerals6 Ecosystem:a community of organisms and their natural environment7FISH :fluorescent in situ hybridization8Hydrothermal vent :a deep-sea warm or hot spring, Infection thread in the formation of root nodules, a cellulosic tube through which Rhizobium cells can travel to reach and infect root cells9 Interspecies hydrogen transfer:the production and subsequent consumption of H2 by different groups of microorganisms that interact closely during anaerobic catabolism10 Lichen:a fungus and an alga (or cyanobacterium) living in symbiotic association11 Microenvironment:the immediate environmental surroundings of a microbial cell or group of cells12Optical tweezers :a laser microscope able to trap single cells and remove them from a cell mixture13Primary producer :an organism that uses light to synthesize new organic material from CO214Reductive dechlorination :removal of Cl as Cl? from an organic compound by reducing the carbon atom from C?Cl to C?H15Root nodule:a tumorlike growth on plant roots that contains symbiotic nitrogen-fixing bacteria16 Ti plasmid:a conjugative plasmid present in the bacterium Agrobacterium tumefaciens that can transfer genes into plants17Anoxic:?18Xenobiotic :a totally synthetic product not naturally occurring in nature19Barotolerant :an organism that can grow under elevated pressures but that grows best at atmospheric pressure20 Biogeochemistry:study of biologically mediated chemical transformations21 Cometabolism:metabolism of a compound in the presence of a second organic compound, which is used as the primary energy source22 Enrichment culture:a means of obtaining cultures of microorganisms from a natural environment by using highly selective culture methods23 Guild:a population of metabolically related microorganisms24In situ:in the environment25 Leaching:solubilization and removal of metals from an ore by microbial attack 26Microbial plastics:biodegradable polymeric materials obtained from microorganisms that have properties similar to those of synthetic plastics27 Mycorrhiza:a symbiotic association between a fungus and the roots of a plant28Oxic:?29 Pyrite:a common iron-containing ore, FeS230Rhizosphere:the region immediately adjacent to plant roots31Rumen:the first vessel in the multichambered stomach of ruminant animals in which cellulose digestion occurs。