选修八Unit 2《Cloning》单元测试3
笔试部分：
I. 单项选择
21. What do you think his proposal _______ we ________ a play at the English party
A. what; put up
B. that; put on
C. which; put up
D. that; put off
22. Ｉwant to _______ a question to the chairman but in a moment I lost my nerve
A. ask
B. reply
C. rise
D. raise
23. He insisted that we ________ rest until we finish the work.
A. not
B. don't
C. can't
D. should
24. I had no idea when and where ________..
A. it happened
B. does it happen
C. it had happened
D. had it happened
25. I really appreciate that your help is of great _________.
A. useful
B. value
C. important
D. worth
26. Sheep can provide us _________ wool, ________ is known to us all.
A. for; that
B. for; which
C. with; which
D. with; that
27. Can you imagine what problem may ________ when human beings are cloned?
A. raise
B. cause
C. arise
D. lead
28. She is too fat, but she can't ________ sweet.
A. remove
B. resist
C. reject
D. object
29. It is widely accepted that animals learn to do things because their certain acts lead to _______.
A. rewards
B. prize
C. awards
D. results
30. ______ is known to us all that Shanghai will host the 2010 World Expo.
A. Which
B. This
C. That
D. It
31. He made a promise ______ he would help me.
A. that
B. which
C. whether
D. why
32. It will not be ________ we can meet again.
A. long before
B. before long
C. soon after
D. shortly after
33. Children can ________ a lot _________ this new kind of school system.
A. benefit; from
B. benefit; with
C. change; from
D. lose; from
34. A good teacher must _________ to his students how important a proper learning method is.
A. get through B, get across C. get away D. get over
35. The news has _______ all over the country _______ the spaceship succeeded in returning to the earth.
A. inspired; which
B. inspiring
C. inspired ; that
D. spread; which
II 完形填空
When I woke next morning, I was dying of _36__. I seemed to have a hole instead of a _37__. I dressed quickly and hurried down to the dinning-room. It was a big room with six tall windows and the ugliest wallpaper I had ever seen ! _38_ , I had
been told the hotel was not beautiful but that you were better __39__ there than in any other hotel; and that was _40__ I wanted just then.
The waiter came hurrying up. Before I came downstairs I had prepared __41__ carefully for what I must __42_. I had looked three times in my dictionary to make sure __43__ “breakfast”really meant “breakfast”. I had tried to get the right _44_ and I had stood in the front of a mirror and twisted my mouth until it ached.
The waiter asked me _45__ I could not understand, but I spoke only my one prepared word “breakfast”. He looked at me in a _46 way. so I repeated it. Still he did not understand. It was _47_ that English people didn't understand their language.. The waiter _48__ his head and went away, but he came back in a minute and brought a tray with tea, bread and butter--- enough to feed a small army--- and went away. But I was hungry, and I left __49__. When the waiter came back I thought his face showed a little __50__, but you can never __51_. What a waiter's face really shows. In another minute he brought _52__ tray with some bacon(熏肉) and some eggs. He _53_ have misunderstood me , but I thought it was no use explaining to people who don't understand their own language , so I just set to work on the bacon and eggs, wondering whether I could possibly clear that plate.
Well, I finished the bacon and eggs. I got up and made my way slowly to my room- at least five pound __54_. I never believed until then that any meal could _55__ me, but on that day I met my Waterloo(滑铁卢).
36. A hunger B. cold C.. anger D. illness
37. A. stone B. head C. breast D. stomach
38. A. Therefore B. Otherwise C. So D. However
39. A. received B. fed C. cared D. eating
40. A. just B. what C. that D. why
41. A. English B. meal C. questions D. myself
42. A. speak B. answer C. say D. explain
43. A. that B. about C. of D. to
44. A. pronunciation B. meaning C. form D. spelling
45. A. whether B. something C. when D. what
46. A. surprised B. friendly C. puzzled D. touched
47. A. unbelievable B. true C. thought D. a pity
48. A waved B. shook C. bowed D. patted
49. A. much B. a little C. nothing D. empty
50. A. pleasure B. surprise C. pride D. satisfaction
51. A. tell B. guess C. design D. express
52. A. other B. another C. more D. me
53. A. should B. might C. would D. must
54. A. lighter B. heavier C. weightier D. more
55.A. hurt B. fat C. defeat D. please
III 阅读理解
A
Every kid wishes to be an adult. But now as grown-ups, some adults find they
cannot leave childhood behind. They become "kidults" (kid+adult). Being a kidult has become a lifestyle-choice among young people across Asia.
Some kidults collect toys they once played with. Hello Kitty, Garfield, and Snoopy have many adult fans around the world. It is not unusual to see a 20-something woman with a big, Garfield-shaped cushion on her sofa or a Hello Kitty mobile phone accessory.
Other kidults still enjoy children's stories and fairy tales. For example, Bloomsbury even published the Harry Potter novels with an adult cover. That way, no one else on the subway will know that an adult is actually reading a children's book! "Kidults can be like vitamins to society. Adults who value their childhood and hold on to pure, child-like emotion may be needed in such a rough and dry society," said Lee Sojung, professor of Foreign Studies at Hankuk University(韩国外国语大学). He added that kidult culture may fill the generation gap between adults and kids. It could give children and their parents books, movies, and cartoon shows to enjoy together. He may be right.
Tim Greenhalgh,a professor,explained that some kidults just refuse to grow up. They value childhood because life in a busy and stressful city frightens them. Kidults would like to forget their age and openly show their fear of society and adulthood.
56. We can infer from the article that _________.
A. Kidults miss their childhood so much that they can't give up their toys
B. Bloomsbury knows some kidults don't want people to know that they are kidults
C. Kidults like to have vitamins very much
D. Some toys are so interesting that even adults like them very much
57. According to the author, being a kidult is good in some way except that _______.
A. it can improve the relationship between parents and their kids
B. it can help kidults feel relaxed
C. it is helpful to solve some social problems
D. it can make people loyal to one's parents
58. According to the writer, _______.
A. being a kidult is nothing wrong
B. kidults have mental problems
C. our society is very dull
D. some people can never grow up
B
If women are mercilessly exploited(剥削) year after year, they have only themselves to blame. Because they tremble at the thought of being seen in public in clothes that are out of fashion, they are always taken advantage of by the designers and the big stores. Clothes which have been worn only a few times have to be put aside because of the change of fashion.
Changing fashions are nothing more than the intentional creation of waste. Many women spend large sums of money each year to replace clothes that have hardly been worn. Women who cannot afford to throw away clothing in this way, waste hours of their time changing their old-fashioned dresses. Skirts are lengthened or shortened; neck-lines are lowered or raised, and so on.
No one can claim that the fashion industry contributes anything really important to
society. Fashion designers are rarely concerned with necessary things like warmth, comfort and durability (耐用). They are only interested in outward appearance and they take advantage of the fact that women will put up with any amount of discomfort, as long as they look right. There can hardly be a man who hasn't at some time in his life smiled at the sight of a woman shaking in a thin dress on a winter day, or picking her way through deep snow in high heeled shoes.
When comparing men and women in the matter of fashion, the conclusions to be drawn are obvious. Do the constantly changing fashions of women's clothes, one wonders, reflect basic qualities of inconstancy and instability? Men are too clever to let themselves be cheated by fashion designers. Do their unchanging styles of dress reflect basic qualities of stability and reliability(稳定耐用)? That is for you to decide.
59. Designers and big stores always make money_________.
A. by mercilessly exploiting women workers in the clothing industry
B. because they are capable of predicting new fashions
C. by constantly changing the fashions in women's clothing
D. because they attach great importance to quality in women's clothing
60. To the writer, the fact that women change their old-fashioned dresses is seen as ___________.
A. a waste of money
B. a waste of time
C. an expression of taste
D. an expression of creativity
61. New fashions in clothing are created for ___________.
A. the commercial exploitation of women
B. the women's strength of character
C. basic qualities of inconstancy and instability
D. an important contribution to society
62. By saying "the conclusions to be drawn are obvious" ( Line One to Line Two, Paragraph Four), the writer means that ___________.
A. women's inconstancy in their choice of clothing is often laughed at
B. women are better able to put up with discomfort
C. men are also exploited greatly by fashion designers
D. men are more reasonable in the matter of fashion
C
While football fans feel strongly angry and shocked about mistakes made by referees (裁判) because they cannot see clearly what has happened, a small German company is quietly pleased.
For Cairos Technologies, mistakes made by referees are the kind of advertising that money cannot buy. The company has developed a tiny chip(芯片) that fits inside a football and determines whether the ball has crossed the goal line, by being able to discover its exact location on the field. The world football organization, FIFA, has shown interest in the technology. It is very possible that the new technology will be used in the 2006 World Cup in Germany.
"We've been testing the technology at the main soccer stadium in Nuremberg for some time and more recently in an under-17 FIFA Cup in Peru," said Gunter Rohmer,
a director of the company. "The technology has performed well, and we're pretty optimistic that it will be used at the games in Germany next year."
The chip only weighs 12 grams, and is placed in the center of the football. It sends 2,000 signals a second to a receiver network of 12 antennas(天线),placed around the field. The receivers then send information about the ball's location to a central computer, and because it works in real time, it can immediately tell the referee whether a goal has been scored. The chip even can tell when the ball crosses the line in mid-air. Oliver Braun, one of the inventors of the chip, says that feedback from German referees was generally positive. Germany sports-wear giant Adidas is also optimistic about using this kind of chip in other ball sports.
FIFA aims to test the technology later this year at another game in Japan before deciding whether or not to introduce it in all 12 stadiums in Germany for next year's World Cup.
63. Carlos Technologies is pleased because ________.
A. football fans are angry with referees
B. their new product can satisfy football fans
C. their new product will have a good market
D. they can sell a lot of football in the future
64. In the first sentence in Paragraph 2,"... mistakes made by referees are the kind of advertising that money cannot buy" means ___________.
A. Carlos Technologies can't afford the high price for advertising their product
B. Mistakes in production are also a kind of advertisement
C. Carios Technologies has already spent a lot of money on advertising their product
D. Referees' mistakes will be of great help for the sale of Carlos Technologies' new product
65. The underlined word "feedback" probably means ___________.
A. information given back by a user
B. advice given by someone
C. supplying food to customers
D. food given back by consumers
66. The new product has drawn attention from some _________.
A. famous people
B. referees
C. big companies
D. countries
D
London--A morning train rides away, across the channel. English kids discuss the Liverpool's football team in a Paris pub.
Some Parisians have started to travel to work in London.
In the 19th century, Charles Dickens compared the two cities, London and Paris, in A Tale of Two Cities. These days, it might be A tale of One City.
As there are few jobs at home over recent years, perhaps 250,000 Frenchmen moved across the channel. With an undersea tunnel, they could travel between cities in three hours. The European Union freed them from immigration and customs.
Paris, rich in beauty, is more stylish. But London feels more full of life, and more fun until the pubs shut down.
"For me, the difference is that London is real, alive," said Trevor Wheeler, a financial expert.
Chantal Jaouen, a professional designer, agrees. "I am French, but I'll stay in London,'' she said.
There is, of course, the other view. Julie Lenoux is a student who moved to London two years ago. "I think people laugh more in Paris," she said.
"Both cities have changed beyond recognition," said Larry Collins, an author and sometimes a Londoner.
Like most people who know both cities well, he finds the two now fit together comfortably.
"I first fell in love with Paris in the 1950s. Things are so much more ordered, and life is better."
But certainly not cheaper.
In some parts of London, rents can be twice those on Avenue Foch in Paris. Deciding between London and Paris requires a lifestyle choice.
Like Daphne Benoit, a French journalism student with perfect English, many young people are happy to be close enough so they don't have to choose.
"I love Paris, my little neighbourhood, the way I can walk around a centre, but life is too organized , "she said. "In London, you can be whoever you want. No one cares."
67. It can be inferred that ___________.
A. Paris and London are the two biggest cities in the world
B. in the 19th century, Dickens told his stories in the two cities
C. London and Paris used to be separated
D. Liverpool is a big city in France
68. According to this passage, which of the following is TRUE?
A. People feel it difficult to find a job in Paris.
B. People can't travel to London without a passport.
C. People can travel to London without a passport.
D. People can find any job in London.
69. According to this passage we know that _________.
A. Parisians enjoy English food more than their own
B. Londoners seldom travel to Paris on holidays
C. both cities have their advantages
D. young people prefer to live in London
70. Which city is better to live in according to the passage?
A. Paris.
B. London.
C. Both cities.
D. It just depends.
71. What's the meaning of the last sentence?
A. People can do everything in London.
B. People will feel lonely in London.
C. People in London enjoy living in different ways.
D. People in London enjoy a lawless life.
E
Now let's look ourselves as a species in relation to ecosystem balance.
Modern scientists believe that humankind, like other animals, evolved(进化) through millions of years of changes and adaptations to the environment and that our most direct evolutionary ancestor was probably an earlier species of the primate(monkey, ape) group. Despite this similarity with other creatures, however, the evolution of humankind differs from that of other species in one important and unique way.
In other species, evolution has led to specialization, both in the species abilities and in its place within the environmental structure. For example, the giraffe is much adapted to feeding on treetops, but it is also specialized and thus limited to feeding on trees and shrubs. Only with great difficulty can it bend down to graze on the ground. Similarly, the anteater is extremely well adapted to eating ants but is unable to catch or eat other animals. The same is true for countless other species. For humankind it is opposite. Our evolution had led to a very generalized ability. Our highly developed intelligence and ability to make and handle tools mean that we can do almost anything. Humans evolved in such a way that we are able to move into every environment on Earth and even into space. No natural competitor offers great resistance, and other natural enemies such as disease have been controlled.
Said another way, we see in humankind a great imbalance between biological potential and environmental resistance. The result is the rapidly increasing world population, frequently referred to as the population explosion. Further, to support our growing population, natural ecosystems are being increasingly displaced by
human habitations, agriculture, and other human supporting activities.
72. From the passage we can infer that in the course of evolution _________.
A. humankind is very important to earlier species, such as the primate group
B. human beings are limited by the environment while animals are not
C. human beings become more and more different from each other
D. humankind has experienced a generalization rather than specialization in ability
73. According to the passage, primate includes such animals as ________.
A. monkeys and anteaters
B. tigers and apes
C. apes and monkeys
D. monkeys and giraffes
74. According to the passage, evolution has made it possible for humans to _______.
A. increase the population
B. resist natural offers
C. go to the moon
D. cure all diseases
75. According to the author, imbalance between biological potential and environ mental resistance has resulted in ________.
A. the population explosion
B. the destruction of human habitations
C. the growth of natural ecosystem
D. the specialization of humans
IV 短文改错
此题要求改正所给短文中的错误。对标有题号的每一行作出判断：如无错误，在该行右边的横线上画一个勾(√)；如有错误(每行只有一个错误)，则按下列情况改正：
该行多一个词：把多余的词用斜线(＼)划掉，在该行右边横线上写出该词，并也用斜线(＼)划掉。
该行缺一个词：在缺词处加一个漏词符号(∧)，在该行右边横线上写出该加的词。
该行错一个词：在错的词下划一横线，在该行右边横线上写出改正后的词。
注意：原行没有错的不要改。
Dear editor,
In our school students are constantly complaining about bad
food in the dining room. It serves with many dishes from 76. ______________ Cantonese to Sichuan hotpot, so the quality isn't good. Some 77. ______________ students said they had found stones or died pests in their food. 78.
______________
The popular joke is that you are really luck if you find nothing 79. ______________ strange or smelly in the food. As the majority of student 80. ______________ have no choice but eat in the dining room, money can still be 81. ______________ made if the dining room takes an effort to improve quality of 82. ______________ food. The dining room should pay attention to providing 83. ______________ better services and tasted food. It is high time that the 84. ______________ dining room changed their idea of management. We are 85. ______________ looking forward to the day.
V 书面表达
假如你是本地报社的一名记者，对新华中学昨天的一场辩论进行报道，写一篇新闻稿。
昨天新华中学的学生就是否允许医学克隆存在的话题展开了激烈的辩论。辩论一方非常坚定地赞成医学克隆。他们指出医学克隆将会给那些因为重病和丧失了行动和思考能力的人带去
福音；解释道这样的操作程序对病人来说无疑将会是万无一失、安全和快捷的；并且举出６７％的美国民众支持医学克隆来支持自己的观点。辩论的另一方则反对医学克隆，他们从两方面说明了理由：第一，克隆仍然存在着安全隐患；第二，为了生成用于医用或研究的胚胎需要反复的尝试，在这一过程中很多潜在的生命会不得不无情扼杀，这一点也正是很多西欧国家反对医学克隆的原因。但是反对方的说服力显然要逊色一些，最终观众投票正方获胜。辩论结束时，现场观众看到了曙光，他们意识到在今天很多严重的疾病在不久的将来有可能找到治愈方法，因此他们非常的兴奋。
_______________________________________________________________________________
________________________________________________________________________________________ ________________________________________________________________________________________ ________________________________________________________________________________________ ________________________________________________________________________________________ ________________________________________________________________________________________ __________________________________
参考答案：
单项填空21—25 BDACB 26—30 CCBAC 31—35 AAABC
完形填空36—40 ADDBA 41—45 DACAA 46—50 CABCB 51—55 ABDBC
阅读理解56—60 BDACB 61—65 ADCDA 66—70 CCACD 71—75 CDCCA
短文改错
76．去掉with 77．so→but 78．died→dead 79．1uck→lucky 80．student →students 81．but后加to 82．quality前加the 83．√84．tasted→tasting ／tasty 85．their→its
书面表达
Yesterday there was a very exciting debate at Xinhua Middle School about whether medical cloning should be allowed or not .After a lively discussion a vote was taken and the side arguing for medical cloning won.
The arguments for medical cloning were very strongly put by the team supporting the motion. They argued that serious illnesses and people who w losing their ability to move or think could be helped by medical cloning. They explained that the procedures would be safe, quick and with no problems for the patients. They quoted that 67% of American citizens are for it to support their idea. Their arguments were very convincing.
The arguments put by the team opposing medical cloning were less strong. They argued in two ways. The first reason was that the procedures were not yet secure.
Their second reason was that it took many tries and many potential animals had to be killed to produce one useable embryo for research. They explained that man European countries don't allow medical cloning research because of this. They quoted Pope John Paul Ⅱ. But their arguments were not so convincing.
At the end of the debate the audience we left with a feeling of hope. They realized that diseases that seem terrible today may be able to be cured soon. Many people were excited and happy when they left they Hall .The writer of this article was among them!
生物选修三综合测试题 Document serial number【NL89WT-NY98YT-NC8CB-NNUUT-NUT108】
选修三综合测试题（一） 一、选择题：（每小题只有一个选项符合题意。） 1．下图为DNA 分子在不同酶的作用下所发生的变化，图中依次表示限制性内切酶、DNA 聚合酶、DNA 连接酶、解旋酶作用的正确顺序是 A.①②③④ B.①②④③ C.①④②③ D.①④③② 2.科学家用小鼠骨髓瘤细胞与B 淋巴细胞融合，得到杂交细胞，经培养可产生大量的单克隆抗体，所用的B 淋巴细胞是来自 A ．骨髓的 B 淋巴细胞 B ．脾脏的能产生抗体的B 淋巴细胞 C ．胸腺的B 淋巴细胞 D ．脾脏经过筛选的，能产生单一抗体的B 淋巴细胞 3.下列过程中，没有发生膜融合的是 A ．植物体细胞杂交 B ．受精过程 C ．氧进入细胞中的线粒体 D ．效应B 细胞产生抗体 4.在植物组织培养过程中，愈伤组织的形成和形态发生是十分关键的一步,而这除需要 必备的营养和一些刺激因素外，还需要有起诱导作用的物质，它是 A ．铜、锌等微量元素 B ．细胞分裂素和生长素 C ．蔗糖和葡萄糖 D ．维生素和氨基酸 5．下列不． 属于动物细胞工程应用的是 A ．大规模生产干扰素,用于抵抗病毒引起的感染 B ．为大面积烧伤的病人提供移植 的皮肤细胞 C ．大规模生产食品添加剂、杀虫剂等 D ．利用胚胎移植技术，加快优良 种畜的繁殖 6．科学家发现将人的干扰素的cDNA 在大肠杆菌中进行表达，产生的干扰素的抗病毒活 性为 106μg ／ml ，只相当于天然产品的十分之一，通过基因定点突变将第17位的半 ─→ ① ─→ ② ─→ ③ ─→ ④
人教版高中英语必修三测试题及答案本试卷分第Ⅰ卷和第Ⅱ卷两部分。卷Ⅰ第 ) 分30共两节，满分(听力第一部分第一节) 分7.5分，满分1.5小题；每小题5共(C、B、A段对话。每段对话后有一个小题，从题中所给的5听下面三个选秒钟的10你都有听完每段对话后，并标在试卷的相应位置。项中选出最佳选项，时间来回答有关小题和阅读下一小题。每段对话仅读一遍。 What can be inferred? ．1 The man is expecting the telephone. ．A The man doesn't usually get calls at this time. ．B The man doesn't believe the woman. ．C Why does the woman call Henry a dreamer? ．2 He has too many dreams. ．A He likes to sleep. ．B He doesn't put his idea into practice. ．C How does the woman feel about the final exam? ．3 Confident. ．B so.－Just so．A Disappointed. ．C What does the woman offer to do for the man? ．4 Give him a map. ．A Give him a ride. ．B Show him another route. ．C What is the man going to do? ．5 Talk to more soldiers. ．A Organize the information. ．B Collect more information. ．C ) 分22.5分，满分1.5
1 / 3 罗城中学高2013届化学学科第二次模块考试 考试时间：90分钟 满分100分 命题人:蒋艳 第I 卷（共50分） 一．选择题：（每小题只有一个选项合符要求，每小题2分，共20分。） 1.元素的性质呈现周期性变化的根本原因是( ) A ．原子半径呈周期性变化 B ．元素的化合价呈周期性变化 C ．第一电离能呈周期性变化 D ．元素原子的核外电子排布呈周期性变化 2．下列原子的电子排布图中，正确的是( ) 3．已知下列元素原子的最外层电子排布式，其中不一定能表示该元素为主族元素的是( ) A ．3s 2 3p 3 B ．4s 2 C ．4s 2 4p 1 D ．3s 2 3p 5 4.下列晶体熔化时不需破坏化学键的是 A ． 晶体硅 B ．食盐 C ．干冰 D ．金属钾 5．某元素原子3p 能级上有一个空轨道，则该元素为( ) A ．Na B ．Si C ．Al D ．Mg 6．下列各项中表达正确的是（ ） A ．硫离子的核外电子排布式 ： 1s 2 2s 2 2p 6 3s 2 3p 6 B ．N 2的结构式： :N ≡N : C ．NaCl 的电子式： D ．CO 2的分子模型示意图： 7.下列事实与氢键有关的是 A ．HF 、HCl 、HBr 、HI 的热稳定性依次减弱 B.水加热到很高的温度都难以分解 C ．CH 4、SiH 4、GeH 4、SnH 4熔点随相对分子质量增大而升高 D ．水结成冰体积膨胀 8．下列分子和离子中中心原子价层电子对几何构型为四面体且分子或离子空间的构型为V 形的是( ) A ．NH 4＋ B ．PH 3 C ．H 3O ＋ D ．OF 2 9．下列不能形成配位键的组合是( ) A ．Ag ＋ 、NH 3 B ．BF 3、NH 3 C ．NH 4+ 、H ＋ D ．Co 3＋ 、CO 10．下列分子或离子中，不存在sp 3 杂化类型的是： A 、SO 42- B 、NH 3 C 、C 2H 6 D 、SO 2 二、选择题（每题有1~2个正确选项，共30分） 11．水星大气中含有一种被称为硫化羰(化学式为COS)的物质。已知硫化羰与CO 2的结构相似，但能在O 2中完全燃烧，下列有关硫化羰的说法正确的是( ) A ．硫化羰的电子式为··S ···· ··C ··O ···· · · B ．硫化羰分子中三个原子位于同一直线上 C ．硫化羰的沸点比二氧化碳的低 D ．硫化羰在O 2中完全燃烧后的产物是CO 2和SO 2 12.下列各组原子中,彼此化学性质一定相似的是 （ ） A.原子核外电子排布式为1s 2 的X 原子与原子核外电子排布式为1s 2 2s 2 的Y 原子 B.原子核外M 层上仅有两个电子的X 原子与原子核外N 层上仅有两个电子的Y 原子 C.2p 轨道上有三个未成对的电子的X 原子与3p 轨道上有三个未成对的电子的Y 原子 D.最外层都只有一个电子的X 、Y 原子 13. 下面的排序不正确的是 （ ） A.晶体熔点由低到高：CF 4 高中生物选修测试题文稿归稿存档编号：[KKUY-KKIO69-OTM243-OLUI129-G00I-FDQS58- 选修1生物技术实践 一、单项选择题(本题包括16小题，每小题3分，共48分) 1．如下图所示，在适宜的温度条件下，在下列装置中都放入干酵母(内有活酵母菌)，其中适于产生酒精的装置是 ( ) 2．下列关于腐乳制作的叙述，错误的是 ( ) A．含水量大于85%的豆腐利于保持湿度，适宜制作腐乳 B．在腐乳制作过程中必须有能产生蛋白酶的微生物参与 C．卤汤中的酒应控制在12%左右 D．装瓶时，应将瓶口通过酒精灯火焰 3．在果酒、果醋和腐乳制作中，都要防止微生物污染，有关叙述正确的是 ( ) A．果醋发酵阶段应封闭充气口，防止杂菌进入 B．腌制腐乳的卤汤中应含有12%左右的酒精以抑制细菌的增殖 C．利用自然菌种发酵果酒时，将封有葡萄汁的发酵瓶进行高压灭菌D．将长满毛霉的豆腐放在瓶中，并逐层加盐，接近瓶口部分的盐要铺薄一些 4．下列关于生物技术实践的叙述中，错误的是 ( ) A．各种微生物的培养基配方不同，但一般都含有水、碳源、氮源和无机盐 B．与斐林试剂检测相比，用尿糖试纸检测尿液中的葡萄糖，特异性更强、灵敏度更高 C．利用PCR技术扩增目的基因时，引物Ⅰ和引物Ⅱ的碱基序列应互补 D．腐乳制作过程中，添加料酒、香辛料和盐，均可以抑制杂菌的生长 5．稀释涂布平板法是微生物培养中的一种常用的接种方法。下列相关叙 述错误的是 ( ) A．操作中需要将菌液进行一系列的浓度梯度稀释 B．需将不同稀释浓度的菌液分别涂布到固体培养基表面 C．不同浓度的菌液均可在培养基表面形成单个的菌落 D．操作过程中对培养基和涂布器等均需进行严格灭菌处理 8．检验培养基是否有杂菌污染的最有效方法是 ( ) A．将未接种的培养基放在实验桌培养 B．将未接种的培养基放在窗台培养 C．将未接种的培养基放在恒温箱中培养 D．将已接种的培养基放在恒温箱中培养 9．植物细胞表现出全能性的必要条件是 ( ) A．给予适宜的营养和外界条件 B．导入其他植物的基因 C．脱离母体并给予适宜的营养和外界条件 D．将成熟的筛管的细胞移到去核的卵细胞中 10．能够测定样品活菌数的方法是 ( ) A．稀释涂布平板法B．直接计数法 C．重量法 D．比浊法 11．在下列选项中，没有采用植物组织培养技术的一项是 ( ) A．利用秋水仙素处理萌发的种子或幼苗，得到多倍体植株 B．利用花药培养得到单倍体植株 C．利用基因工程培育抗棉铃虫的棉花植株 D．利用细胞工程培育“番茄－马铃薯”杂种植株 12．关于发酵过程产物检验的说法，正确的是 ( ) A．果汁发酵是否产生酒精，可用NaOH来检验 B．检验醋酸产生的简单易行的方法是品尝或用pH试纸鉴定 C．泡菜制作过程中亚硝酸盐的含量不能测定 最新高中英语必修三测试题全套及答案 （人教新课标） Unit 1 单元测试题 阅读理解（共两节，满分40分） 第一节（共15小题；每小题2分，满分30分） 阅读下列短文，从每题所给的四个选项（A、B、C和D）中，选出最佳选项。 A Walk into the California home of Anne Belles and her husband, Jim Silcock, and you?ll see kids everywhere playing video games, doing homework, and getting ready for dinner. There are 30 boys in this house and Anne Belles is their mom. Belles has wanted to help children since she was a kid. “I was intrigued by the movie Oliver! in the 1960s, a musical based on the Charles Dickens novel Oliver Twist. I told my mom, …That?s what I want to do. …” Anne?s boys are from 3 to 25 years old. All of them are challenged in some way. “They each have special needs — physically, mentally (精神上), or at school,” says Belles. Every day, a small army of childcare workers, nurses, and volunteers comes in to help cook and clean, wash 30 loads of laundry a day, and take care of health needs. To find out how much such a large family costs, we followed Jim Silcock to the grocery store. He spent $880 on food for one week. Every month they spend $2,000 to run five cars, $15,000 for the fourteen paid helpers, and more than $10,000 on medical costs. The family receives $26,000 a month from the state government, and makes some money from a family business. All the money is spent on the children; having new clothes and fancy cars isn?t important to Belles. How do the kids feel? 17-year-old Anthony says, “The family is there whenever I need something ... I feel like I am loved.” “Everything I?m doing now is what I wanted to happen in my life,” says Anne Belles. “So, no regrets; this is perfect. I couldn?t ask for it to be better — maybe a bigger house, you know, would be nice.” 21. The underlined word “intrigued” in the first paragraph means “_____”. A. fooled B. attracted C. frightened D. disappointed 22. The boys Anne has raised _____. A. are all ready to accept a challenge B. all like Oliver Twist C. all have disabilities 化学选修3 期末考试 一、选择题（单选，每小题3分，共48分） 1．下列对化学反应的认识错误的是（） A．会引起化学键的变化 B．会产生新的物质 C．必然引起物质状态的变 D．必然伴随着能量的变化2．对2与2说法正确的是（） A.都是直线形结构 B.中心原子都采取杂化轨道 原子和C原子上都没有孤对电子2为V形结构，2为直线形结构3．下列叙述中正确的是（） A．金属的熔点和沸点都很高 B．H2O2、5都是含有极性键的非极性分子 C．、、、的酸性依次增强 D．H2O是一种非常稳定的化合物，这是由于氢键所致 4．下列无机含氧酸的酸性强弱比较正确的是（） 2>33>H34 C>223>3 5.已知X、Y是主族元素，I为电离能，单位是。根据下表所列数据判断错． 误的是（） A.元素X的常见化合价是+1价 B.元素Y是ⅢA族的元素 C.元素X与氯形成化合物时，化学式可能是 D.若元素Y处于第3周期，它可与冷水剧烈反应 6．下列说法错误的是（） A．s轨道呈圆形，p轨道呈哑铃形 B．元素在元素周期表的区 C．1.5g 3+中含有的电子数为0.8 D．中的碱基互补配对是通过氢键来实现的 7. 下列说法中错误的是（） A．根据对角线规则，铍和铝的性质具有相似性 B．在H3、4+和[(3)4]2+中都存在配位键 C．元素电负性越大的原子，吸引电子的能力越强 D．P4和4都是正四面体分子且键角都为109o28ˊ 8. 用价层电子对互斥理论（）预测H2S和2的立体结构，两个结论都正确的是( ) A．直线形；三角锥形 B．V形；三角锥形 C．直线形；平面三角形 D．V形；平面三角形 9.为（） A.485 · －1 10. 对充有氖气的霓红灯管通电，灯管发出红色光。产生这一现象的主要原因（） A．电子由激发态向基态跃迁时以光的形式释放能量 B．电子由基态向激发态跃迁时吸收除红光以外的光线 C．氖原子获得电子后转变成发出红光的物质 D．在电流作用下，氖原子与构成灯管的物质反应 11. 在乙炔分子中有3个σ键、两个π键，它们分别是（） A．杂化轨道形成σ键、未杂化的两个2p轨道形成两个π键，且互相垂直 B．杂化轨道形成σ键、未杂化的两个2p轨道形成两个π键，且互相平行 C．之间是形成的σ键，之间是未参加杂化的2p轨道形成的π键D．之间是形成的σ键，之间是未参加杂化的2p轨道形成的π键12.已知氯化铝易溶于苯和乙醚，其熔点为190℃，则下列结论错误的是（） A.氯化铝是电解质 B.固体氯化铝是分子晶体 C.可用电解熔融氯化铝的办法制取金属铝 D.氯化铝为非极性分子 13．关于原子轨道的说法正确的是（） A.凡是中心原子采取3杂化轨道成键的分子其几何构型都是正四面体 4分子中的3杂化轨道是由4个H原子的1s 轨道和C原子的2p轨道混 合形成 3杂化轨道是由同一个原子中能量相近的s 轨道和p轨道混合起来形成的一组新轨道 D.凡3型的共价化合物，其中中心原子A均采用3杂化轨道成键 14. 下列说法或表示方法中正确的是 选修教材练习 一、选择题 1.（全国卷1）下列关于植物体细胞杂交或植物细胞质遗传的叙述，错误的是 A 利用植物体细胞杂交技术可以克服生殖隔离的限制，培育远缘杂种。 B不同植物原生质体融合的过程属于植物体细胞杂交过程。 C两个不同品种的紫茉莉杂交，正交反交F1的表现型一致。 D两个不同品种的紫茉莉杂交，F1的遗传物质来自母本的多余父本的。 2（重庆）下表有关基因表达的选项中，不可能的是 D 基因表达的细胞表达产物 A 细菌抗虫蛋白基因抗虫棉叶肉细胞细菌抗虫蛋白 B 人A 酪氨酸酶基因正常人皮肤细胞人酪氨酸酶 C 动物胰岛索基因大肠杆菌工程菌细胞动物胰岛索 D 兔血红蛋白基因兔成熟红细胞兔血红蛋白 3．（浙江）用动、植物成体的体细胞进行离体培养，下列叙述正确的是 A．都需用CO2培养箱B．都须用液体培养基 C．都要在无菌条件下进行D．都可体现细胞的全能性 4．（浙江）下列关于基因工程的叙述，错误的是 A．目的基因和受体细胞均可来自动、植物或微生物 B．限制性核酸内切酶和 DNA 连接酶是两类常用的工具酶 C．人胰岛素原基因在大肠杆菌中表达的胰岛素原无生物活性 D．载体上的抗性基因有利于筛选含重组 DNA 的细胞和促进目的基因的表达 5．（江苏）下列关于哺乳动物胚胎发育和胚胎工程的叙述，正确的是 A．卵裂期胚胎中细胞数目和有机物总量在不断增加 B．胚胎分割时需将原肠胚的内细胞团均等分割 C．胚胎干细胞具有细胞核大、核仁小和蛋白质合成旺盛等特点 D．胚胎干细胞是一类未分化细胞，可从早期胚胎中分离获取 6．（广东 B 卷）钱永健先生因在研究绿色荧光蛋白方面的杰出成就而获 2008 年诺贝尔奖。在某种生物中检测不到绿色荧光，将水母绿色荧光蛋白基因转入该生物体内后，结果可以检测到绿色荧光。由此可知 A．该生物的基因型是杂合的 B．该生物与水母有很近的亲缘关系 C．绿色荧光蛋白基因在该生物体内得到了表达 D．改变绿色荧光蛋白基因的 1 个核苷酸对，就不能检测到绿色荧光 二、非选择题： 必修三测试卷 本试卷分第一卷(选择题)和第二卷(非选择题)两部分。满分150分。 考试时间120分钟。 第Ⅰ卷(选择题共100分) 第一部分听力(共两节，满分30分) 第一节（共5小题；每小题1.5分，满分7.5分） 听下面5段对话。每段对话后有一个小题，从题中所给的A、B、C三个选项中选出最佳选项，并标在试卷的相应位置。听完每段对话后，你都有10秒钟的时间来回答有关小题和阅读下一小题。每段对话仅读一遍。 第二部分阅读理解（共两节，满分40分） 第一节（共15小题；每小题2分，满分30分） 阅读下列短文，从每题所给的四个选项(A、B、C和D)中，选出最佳选项，并在答题卡上将该项涂黑。 A Wonderful MOVIE！！！ The Ant Bully Actor: Julia Boberts-Hova ( voice )Nicolas Cage-Zoc ( voice ) Language: English Run Time: 1 Hour 25 Minutes Price: Mon.- Fri. - $30 ( VIP: $18 ) $15 ( Children under 7 ) Sat.- Sun. -$40 ( VIP: $28 ) $20 ( Children under 7 ) Lucas Nickle is often bullied(欺负) by the big boy in his neighborhood. Because he cannot fight back, he takes out his anger on an anthill(蚁丘) in his front yard. He stomps(践踏) it, squirts(喷射) it with his water gun, and floods it. One ant named Zoc makes Lucas as little as an ant and Lucas has to work and live in the ants' country. During his stay with the ants, Lucas learns the value of friendship and teamwork, and finally gains the things he wants most: friendship and the courage to stand up for himself. Comments(评论): I really enjoyed this movie. It's a fun movie for the whole family. I had really good laughs and enjoyed being there. –James I went to see this film today with my 8-year-old daughter, really without knowing too much about it. My daughter and I were pleasantly surprised at how funny it was. We just couldn't help laughing. I would recommend this film to the whole family. –Mary I have seen several movies of this kind, like Antz or Bugs Life, but this one blows them both away as having greater movie qualities. The film is so well made-some of the scenes are unbelievably beautiful. Unit 2 Healthy eating 第Ⅰ卷 第二部分：阅读理解（共两节，满分40分） 第一节（共15小题；每小题2分，满分30分） 阅读下列短文，从每题所给的四个选项（A、B、C和D）中，选出最佳选项。 A Sundays, I walk to the supermarket. Mother hands me the grocery list and puts money in my pocket, hoping it will be enough. She's had a hard day, and I've had a hard week. Nothing out of the ordinary happens when I get to the store. I grab the bread, some milk, and other things on the list. As I turn to head out, I see a beautiful dress in the window. I turn away, bitter that I could never own such a dress. Outside, I cannot stop thinking about that pretty dress. It's not fair that I can never have what I want. I work so hard to help my family and yet I get nothing in return, just another list to do. In my anger, I fail to realize the apples are rolling across the road. Suddenly, I see a pair of hands, offering me an apple. Looking up, I see the tanned (晒黑的) face of this stranger. His clothes are mismatched, borrowed or stolen. But his eyes are soft and kind. “Thanks,” I say. No other words are spok en as he continues to help me. I tell him “thank you” one more time and am on my way because I have many other things to finish. Suddenly, he says, “Have a good day, ma'am.” And then he gives me the biggest smile I have ever seen. Right then, he looks years younger — and I feel a fool. 高中化学选修三模块测试题 [选题细目表] 一、选择题(本题包括7个小题，每小题6分，共42分，每小题仅有一个选项符合题意) 1．下列说法中正确的是() A．在分子中，两个成键的原子间的距离叫键长 B．分子中含有共价键，则至少含有一个σ键 C．含有非极性键的分子一定是非极性分子 D．键能越大，键长越长，则分子越稳定 解析：键长是形成共价键的两个原子之间的核间距，A错；单键一定是σ键，双键由1个σ键和1个π键构成，三键由1个σ键和2个π键构成，故分子中含有共价键，则至少含1个σ键，B正确；含非极性键的分子不一定是非极性分子，如H2O2，C错；键能越大，键长越短，分子越稳定，D错。 答案：B 2．下列有关乙炔分子的说法正确的是() A．分子中碳原子采取sp2杂化 B．碳原子的杂化轨道只用于形成π键 C．分子中含3个σ键、2个π键 D．碳碳σ键比π键重叠程度小，易断裂 解析：乙炔分子中碳原子采取sp杂化，杂化轨道只用于形成σ键，未参与杂化的轨道可用于形成π键，A、B错误；乙炔分子中含2个C—H σ键、1个C—C σ键和2个π键，C正确；σ键是头碰头的重叠，π键是肩并肩的重叠，σ键比π键重叠程度大，σ键比π键稳定，D错。 答案：C 3．(2016·衡水模拟)用价层电子对互斥理论(VSEPR)可以预测许多分子或离子的空间构型，有时也能用来推测键角大小，下列判断正确的是() A．SO2、CS2、HI都是直线形的分子 B．BF3键角为120°，SnBr2键角大于120° C．CH2O、BF3、SO3都是平面三角形的分子 D．PCl3、NH3、PCl5都是三角锥形的分子 解析：A项，SO2是V形分子；CS2、HI是直线型的分子，错误；B项，BF3键角为120°，是平面三角形结构；而Sn原子价电子是4，在SnBr2中两个价电子与Br形成共价键，还有一对孤对电子，对成 选修三综合测试题（一） 一、选择题：（每小题只有一个选项符合题意。） 1．下图为DNA 分子在不同酶的作用下所发生的变化，图中依次表示限制性内切酶、DNA 聚合酶、DNA 连接酶、解旋酶作用的正确顺序是 A.①②③④ B.①②④③ C.①④②③ D.①④③② 2.科学家用小鼠骨髓瘤细胞与B 淋巴细胞融合，得到杂交细胞，经培养可产生大量的单克隆抗体，所用的B 淋巴细胞是来自 A ．骨髓的 B 淋巴细胞 B ．脾脏的能产生抗体的B 淋巴细胞 C ．胸腺的B 淋巴细胞 D ．脾脏经过筛选的，能产生单一抗体的B 淋巴细胞 3.下列过程中，没有发生膜融合的是 A ．植物体细胞杂交 B ．受精过程 C ．氧进入细胞中的线粒体 D ．效应B 细胞产生抗体 4.在植物组织培养过程中，愈伤组织的形成和形态发生是十分关键的一步,而这除需要必备的营养和一些刺激因素外，还需要有起诱导作用的物质，它是 A ．铜、锌等微量元素 B ．细胞分裂素和生长素 C ．蔗糖和葡萄糖 D ．维生素和氨基酸 5．下列不． 属于动物细胞工程应用的是 A ．大规模生产干扰素,用于抵抗病毒引起的感染 B ．为大面积烧伤的病人提供移植的皮肤细胞 C ．大规模生产食品添加剂、杀虫剂等 D ．利用胚胎移植技术，加快优良种畜的繁殖 6．科学家发现将人的干扰素的cDNA 在大肠杆菌中进行表达，产生的干扰素的抗病毒活性为 106μg ／ml ，只相当于天然产品的十分之一，通过基因定点突变将第17位的半胱氨酸改变成丝氨酸，结果使大肠杆菌中生产的β一干扰素的抗病性活性提高到108μg ／ml ，并且比天然β一干扰素的贮存稳定性高很多。此项技术属于 A ．细胞工程 B ．蛋白质工程 C ．胚胎工程 D ．基因工程 ─→ ① ─→ ② ─→ ③ ─→ ④ 湖北黄石二中选修3第一章《原子结构与性质》单元测试题 试卷满分：150分时间：120分钟命题人：高存勇2010.12.23 选择题(每小题有一个或者两个正确答案,每小题2分,共60分) 1.第三周期元素的原子,其最外层p能级上仅有一个未成对电子,它最高价氧化物对应的水化物的酸根离子是 A.RO－3B．RO－5C．RO2－4D．RO－4 2.下列各组元素,按原子半径依次减小,元素第一电离能逐渐升高的顺序排列的是 A.K、Na、Li B．Al、Mg、Na C.N、O、C D．Cl、S、P 3.基态原子的第5电子层只有2个电子,则该原子的第四电子层中的电子数肯定为 A.8个B．18个C．8～18个D．8～32个 4.下列关于稀有气体的叙述不正确的是 A.各原子轨道电子均已填满 B.其原子与同周期ⅠA、ⅡA族阳离子具有相同的核外电子排布 C.化学性质非常不活泼 D.同周期中第一电离能最大 5.下列电子排布式中,原子处于激发状态的是 A.1s22s22p5 B.1s22s22p43s2 C.1s22s22p63s23p63d44s2 D.1s22s22p63s23p63d34s2 6.下列元素中价电子排布不正确的是 A.V：3d34s2 B.Cr：3d44s2 C.Ar：3s23p6 D.Ni：3d84s2 7.下列说法中正确的是 A.因为p轨道是“8”字形的,所以p电子走“8”字形 B.主量子数为3时,有3s、3p、3d、3f四个轨道 C.基态铜原子有8个能级 D.原子轨道与电子云都是用来形象描述电子运动状态的 8.A和M为两种元素,已知A位于短周期,且A2－与M＋的电子数之差为8,则下列说法正确的是 A.A和M原子的电子总数之和可能是11 B.A和M的原子序数之和为8 C.A和M原子的最外层电子数之和为8 D.A和M原子的最外层电子数之差为7 9.具有下列电子层结构的原子,其对应元素一定属于同一周期的是 A.两种原子的电子层上全部都是s电子 B.3p上只有一个空轨道的原子和3p亚层上只有一个未成对电子的原子 C.最外层电子排布为2s22p6的原子和最外层电子排布为2s22p6的离子 D.原子核外M层上的s亚层和p亚层都填满了电子,而d轨道上尚未有电子的两种原子 10.同一主族的两种元素的原子序数之差可能为 A.6 B．12 C．26 D．30 11.A、B属于短周期中不同主族的元素,A、B原子的最外层电子中,成对电子和未成对电子占据的轨道数相等,若A元素的原子序数为a,则B元素的原子序数为 A.a－4B．a－5C．a＋3D．a＋4 12.用R代表短周期元素,R原子最外层的p能级上的未成对电子只有2个。下列关于R的描述中正确的是 A.R的氧化物都能溶于水 B.R的最高价氧化物所对应的水化物都是H2RO3 C.R都是非金属元素 D.R的氧化物都能与NaOH溶液反应 13.A、B、C、D四种短周期元素的原子半径依次减小,A与C的核电荷数之比为3∶4,D 能分别与A、B、C形成电子总数相等的分子X、Y、Z。下列叙述正确的是 A.X、Y、Z的稳定性逐渐减弱 B.A、B、C、D只能形成5种单质 C.X、Y、Z三种化合物的熔、沸点逐渐升高 D.自然界中存在多种由A、B、C、D四种元素组成的化合物 14.国际无机化学命名委员会将长式元素周期表原先的主、副族及族序序号取消,从左往右改为第18列,碱金属为第1列,稀有气体为第18列。按这个规定,下列说法不正确 ．．．的是 A.只有第2列元素的原子最外层有2个电子 B.第14列元素形成的化合物种类最多 C.第3列元素种类最多 D.第16、17列元素都是非金属元素 基因工程测试题 姓名____________ 一、选择题 1．已知某种限制性内切酶在一线性DNA分子上有3个酶切位点，如图中箭头所指，如果该线性DNA分子在3个酶切位点上都被该酶切断，则会产生a、b、c、d四种不同长度的DNA片段。现在多个上述线性DNA分子，若在每个DNA分子上至少有1个酶切位点被该酶切断，则从理论上讲，经该酶切后， 这些线性DNA分子最多能 产生长度不同的DNA片段 种类数是( c ) A．3 B．4 C．9 D．12 2．下图是将人的生长激素基因导入细菌B细胞内制造“工程菌”的示意图。已知细菌B细胞内不含质粒A，也不含质粒A上的基因。判断下列说法正确的是( c ) A．将重组质粒导入细菌B常用的方法是显微注射法钙离子处理法B．将完成导入过程后的细菌涂布在含有氨苄青霉素的培养基上，能生长的只是导入了重组质粒的细菌普通质粒A和重组质粒都含有抗氨苄青霉素基因。都能生长因为目的基因在四环素 C．将完成导入过程后的细菌涂布在含有四环素的培养基上，能生长的就是导入了质粒A 的细菌导入重组质粒的细菌不能生长，因为目的基因插在抗四环素基因中，抗四环素基因的结构被破坏。 D．目的基因成功表达的标志是受体细胞能在含有氨苄青霉素的培养基上生长受体细胞通过转录、翻译合成相应的蛋白质，即人的生长激素。 3．下列关于基因工程的叙述，错误的是（D ） A．目的基因和受体细胞均可来自动、植物或微生物 B．限制性核酸内切酶和DNA连接酶是两类常用的工具酶 C．人胰岛素原基因在大肠杆菌中表达的胰岛素原无生物活性 D．载体上的抗性基因有利于筛选含重组DNA的细胞和促进目的基因的表达基因工程中目的基因和受体细胞均可来自动、植物或微生物；常用的工具酶是限制性核酸内切酶和DNA连接酶；人胰岛素原基因在大肠杆菌中表达的胰岛素原无生物活性,只有经过一定的物质激活以后,才有生物活性.载体上的抗性基因主要是有利于筛选含重组DNA的细胞,不能促进目的基因的表达.所以D错误. 4.将ada（腺苷酸脱氨酶基因）通过质粒pET28b导入大肠杆菌并成功表达腺苷酸脱氨酶。下列叙述错误的是（C ） A、每个大肠杆菌细胞至少含一个重组质粒 B、每个重组质粒至少含一个限制性核酸内切酶识别位点 C、每个限制性核酸内切酶识别位点至少插入一个ada不同的限制性核酸内切酶识别位点不同，不是每个限制性核酸内切酶的识别位点都能插入ada。 D、每个插入的ada至少表达一个腺苷酸脱氨酶分子 5.北极比目鱼中有抗冻基因，其编码的抗冻蛋白具有11个氨基酸的重复序列，该序列重复次数越多，抗冻能力越强，下图是获取转基因抗冻番茄植株的过程示意图，有关叙述正确的是（B ）农杆菌转化法 A、过程①获取的目的基因，可用于基因工程和比目鱼基因组测序图中①是获取目的基因的过程，获取的目的基因，可用于基因工程，但不能用于比目鱼基因组测序，故A错误； B、多个抗冻基因编码区依次相连成能表达的新基因，不能得到抗冻性增强的抗冻蛋白将多个抗冻基因编码区相连形成的能表达的新基因可能不再是抗冻基因，所以可能得不到抗冻性增强的抗冻蛋白，故B 正确；， C、过程②构成的重组质粒缺乏标记基因，需要转入农杆菌才能进行筛选②是基因表达载体的构建过程，基因表达载体通常由启动子、目的基因、终止子和标记基因构成，其中标记基因的作用是筛选重组质粒，利用农杆菌转化法只能将质粒导入受体细胞，不能对重组质粒进行筛选，故C错误； D、应用DNA探针技术，可以检测转基因抗冻番茄植株中目的基因的存在及其完全表达利用DNA探针技术检测目的基因是否导入到受体细胞，利用抗原-抗体杂交技术检测目的基因是否表达出来，故D错误．6.在用基因工程技术构建抗除草剂的转基因烟草过程中，下列操作错误的是（A ） A 用限制性核酸内切酶切割烟草花叶病毒的核酸限制性核酸内切 酶只能识别DNA的核苷酸序列,并在特定的位点切割,而烟草花叶病毒的核酸是RNA,限制性核酸内切酶对其不能发挥作用. B 用DNA连接酶连接经切割的抗除草剂基因和载体 C 将重组DNA分子导入原生质体 D 用含除草剂的培养基筛选转基因烟草细胞目的基因为抗除草剂 基因，所以成功导入目的基因的细胞具有抗除草剂的能力，筛选时应该用还有除草剂的培养基筛选转基因细胞 7.下列关于基因工程的叙述，正确的是: ( D ) A.基因工程往往以细菌抗药性基因为目的基因基因工程经常以抗菌素抗性基因为标记基因，便于重组质粒的筛选，A错误； B.重组DNA的形成和扩增是在细胞内完成的比如PCR技术在体外 C.基因工程育种能够定向地改造生物性状，快速形成新物种定向地改造生物的遗传性状，培育新的农作物优良品种的生物技术 D.限制性内切酶和DNA连接酶是构建重组DNA必需的工具酶8．下列有关基因工程和蛋白质工程的叙述，正确的是( B ) A．基因工程需对基因进行分子水平操作，蛋白质工程不对基因进行操作基因工程和蛋白质工程都是分子水平操作，且直接操作对象都是基因。 B．基因工程合成的是天然存在的蛋白质，蛋白质工程合成的可以不是天然存在的蛋白质基因工程合成的是天然存在的蛋白质，而蛋白质工程合成的可以是自然界不存在的蛋白质，但不是只能合成天然不存在的蛋白质。 C．基因工程是分子水平操作，蛋白质工程是细胞水平（或性状水平）操作基因工程和蛋白质工程都是分子水平操作 D．基因工程完全不同于蛋白质工程蛋白质工程是以蛋白质的结构规律及其与生物功能的关系作为基础，通过基因修饰或基因合成，对现有蛋白质进行基因改造，或制造一种新的蛋白质，以满足人类的生产和生活的需要。所以蛋白质工程是在基因工程的基础上延伸出来的第二代基因工程。 9．PCR是一种体外迅速扩增DNA片段的技术，下列有关PCR过程的叙述，不正确的是( C ) A．变性过程中破坏的是DNA分子内碱基对之间的氢键跟解旋酶的作用相同 B．复性过程中引物与DNA模板链的结合是依靠碱基互补配 对原则完成 C．延伸过程中需要DNA聚合酶、ATP、四种核糖核苷酸四种脱氧核糖核苷酸热稳定DNA聚合酶 D．PCR与细胞内DNA复制相比所需要酶的最适温度较高 10.“工程菌”是指（C） A．用物理或化学方法诱发菌类自身某些基因得到高效表达的菌类细胞株系用基因工程的方法，使外源基因得到高效表达的菌类细胞株系一般称为“工程菌” B．用遗传工程的方法，使同种不同株系的菌类杂交，得到的新细胞株系 C．用基因工程的方法，使外源基因得到高效表达的菌类细胞株系 D．从自然界中选取能迅速增殖的菌类 二、非选择题 11.为扩大可耕地面积，增加粮食产量，黄河三角洲等盐碱地的开发利用备受关注。我国科学家应用耐盐基因培育出了耐盐水稻新品系。 植 线性DNA分子的酶切示 意图 人教版高中英语必修三测试题及答案 本试卷分第Ⅰ卷和第Ⅱ卷两部分。 第Ⅰ卷 第一部分听力(共两节，满分30分) 第一节(共5小题；每小题1.5分，满分7.5分) 听下面5段对话。每段对话后有一个小题，从题中所给的A、B、C三个选项中选出最佳选项，并标在试卷的相应位置。听完每段对话后，你都有10秒钟的时间来回答有关小题和阅读下一小题。每段对话仅读一遍。 1．What can be inferred? A．The man is expecting the telephone. B．The man doesn't usually get calls at this time. C．The man doesn't believe the woman. 2．Why does the woman call Henry a dreamer? A．He has too many dreams. B．He likes to sleep. C．He doesn't put his idea into practice. 3．How does the woman feel about the final exam? A．Just so－so.B．Confident. C．Disappointed. 4．What does the woman offer to do for the man? A．Give him a map. B．Give him a ride. C．Show him another route. 5．What is the man going to do? A．Talk to more soldiers. B．Organize the information. C．Collect more information. 第二节(共15小题；每小题1.5分，满分22.5分)高中生物选修测试题
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