同种异体组织工程软骨修复兔气管软骨缺损实验研究

基因转染骨髓间充质干细胞复合同种异体骨修复绵羊极限骨缺损王小志;何惠宇;杨楠;杨泽辉;胡杨【摘要】BACKGROUND:Many in vivo and in vitro experiments indicate that implantation of exogenous basic fibroblast growth factor can significantly promote the process of bone formation, but the in vivo degradation of exogenous basic fibroblast growth factor affects the therapeutic efficacy. <br> OBJECTIVE:To observe the effects of basic fibroblast growth factor-transfected mesenchymal stem cells which transfected using molecular biology techniques combined with al ogeneic bone in the repair of critical-size bone defects in sheep. <br> METHODS:Al ogeneic bone with basic fibroblast growth factor-transfected bone marrow mesenchymal stem cells, bone marrow mesenchymal stem cells combined with al ogeneic bone material stents, al ograft bone material,β-tricalcium calcium material were respectively implanted into critical-size bone defects in sheep. After 4, 8 and 12 weeks, histological and immunohistochemical staining was performed. <br> RESULTS AND CONCLUSION:At 12 weeks after implantation of al ogeneic bone with basic fibroblast growth factor-transfected bone marrow mesenchymal stem cells as tissue engineering bone, there were many cartilage-like structures in the operative binding region and a large amount of osteoblast-like cells in the center of operative region, and there was more material degradation in the entire operative area as compared with other groups;there were fibrousconnective tissues ful of the pores, and osteoclast-like cells were commonly seen&nbsp;around the implant material;bone sialoprotein and col agen type Ⅰ expression were strongly positive. In the other three groups, although the cartilage-like structure appeared in the binding region, dead bone structure was found in the central area, and bone sialoprotein and type Ⅰ col agen expression was weak. These findings indicate that al ogeneic bone with basic fibroblast growth factor-transfected bone marrow mesenchymal stem cells can basical y repair critical-size bone defects in sheep.%背景：多项体内外实验表明外源性植入碱性成纤维细胞生长因子能明显促进骨形成过程，但外源性碱性成纤维细胞生长因子在体内易降解，影响疗效。

《中国组织工程研究》 Chinese Journal of Tissue Engineering Research文章编号:2095-4344(2019)14-02261-08 2261·综述·www.CRTER .org白玉龙，男，1989年生，甘肃省张掖市人，汉族，主要从事组织再生医用材料研究。

通讯作者：赵彦涛，副研究员，解放军总医院第一附属医院骨科，北京市 100048；北京市骨科植入医疗器械工程技术研究中心，北京市 100048文献标识码:A稿件接受：2018-12-17Bai Yulong, Department of Orthopedics, First Affiliated Hospital of the General Hospital of PLA, Beijing 100048, China; Beijing Engineering Research Center of Orthopedics Implants, Beijing 100048, ChinaCorresponding author: Zhao Yantao, Associate researcher, Department of Orthopedics, First Affiliated Hospital of the General Hospital of PLA, Beijing 100048, China; Beijing Engineering Research Center of Orthopedics Implants, Beijing 100048, China同种异体及异种组织修复材料：如何选用适宜的病毒灭活工艺白玉龙1，2，高玉凤1，2，衷鸿宾1，2，赵彦涛1，2，郭睿洲1，2，李 利1，2 (1解放军总医院第一附属医院骨科，北京市 100048；2北京市骨科植入医疗器械工程技术研究中心，北京市 100048)DOI:10.3969/j.issn.2095-4344.1649 ORCID: 0000-0002-8774-9027(白玉龙)文章快速阅读：文题释义：同种异体及异种组织修复材料：通过合法/正规途径获取的同种(捐献者)及异种(动物)组织，经过深低温冷冻、脱细胞、灭菌等工艺处理制备而成的用于人体组织缺损修复的生物材料，硬组织修复材料，如：同种异体骨材料、脱钙骨基质、异种煅烧骨等；软组织修复材料，如：同种/异种脱细胞真皮、同种异体神经、肌腱、羊膜等。

同种异体骨与关节移植的现状发表时间：2015-02-04T16:46:58.113Z 来源：《医药前沿》2014年第27期供稿作者：黄美全谢松林[导读] 随着人工关节假体材料与技术的进步与发展，人体大关节的问题已基本解决，如膝关节、髋关节，肩肘关节置换也有相关报道。

黄美全谢松林(南华大学附属南华医院手足外科湖南衡阳 421001)【摘要】对于关节毁损的治疗一直是困扰骨科临床的一大难题。

人工假体关节置换是目前修复重建关节缺损的有效手段,但对于我们运动强度较大的青少年患者并不适用。

自体关节移植不产生排斥反应,是修复关节毁损的理想方法。

但供体来源紧缺，限制了其在临床上的使用，异体关节来源广泛,具有类似的自然结构、形状、强度、骨诱导能力，可以充分满足临床需要。

经深低温冷冻处理的异体骨已成功地应用于关节修复及关节移植手术，本文综述近几年的对同种异体骨与关节的特殊处理的措施，以及临床上对同种异体关节移植后远期疗效分析。

此外,还简要描述临床上同种异体关节移植的未来及展望。

【关键词】同种异体低温冷冻关节移植【中图分类号】R687 【文献标识码】A 【文章编号】2095-1752（2014）27-0343-02创伤性骨与关节端缺损治疗一直是我们骨科临床上的难点。

创伤导致骨关节端缺损后如何修复重建毁损的关节结构与功能?二十世纪70年代,西方以同种异体骨移植治疗骨关节端缺损取得了初步的进展[1]。

随着人工关节假体材料与技术的进步与发展，人体大关节的问题已基本解决，如膝关节、髋关节，肩肘关节置换也有相关报道。

但手部关节如掌指关节、指间关节僵硬缺损的治疗以及远期的关节退化，一直是临床常见而又棘手的问题。

同种异体关节移植术后关节软骨塌陷与吸收。

如何通过相应的处理得到一个有利于软骨成活且免疫原性低的异体关节移植材料成为急需解决的问题。

1 同种异体骨与关节的降低免疫原性的预处理的方法:(1)低温冷冻法：样本由常温状态浸入冷冻液(主要由2.0mol/L二甲基亚砜组成)，经过3个梯度降温至深低温（-70℃），平衡60min，直接投入液氮中。