Comparative studies on biomass production, life cycles
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COMMISSION DELEGATED REGULATION (EU) No 812/2013of 18 February 2013supplementing Directive 2010/30/EU of the European Parliament and of the Council with regard to the energy labelling of water heaters, hot water storage tanks and packages of water heater andsolar device(Text with EEA relevance)THE EUROPEAN COMMISSION,Having regard to the Treaty on the Functioning of the European Union,Having regard to Directive 2010/30/EU of the European Parliament and of the Council of 19 May 2010 on the indi cation by labelling and standard product information of the consumption of energy and other resources by energy-related products ( 1 ), and in particular Article 10 thereof, Whereas:(1) Directive 2010/30/EU requires the Commission to adoptdelegated acts as regards the labelling of energy-related products that have a significant potential for energy savings but exhibit a wide disparity in performance levels with equivalent functionality.(2) The energy consumed by water heaters and hot waterstorage tanks accounts for a significant share of the total energy demand in the Union, and water heaters and hot water storage tanks with equivalent functionality exhibit a wide disparity in terms of water heating energy effi ciency and standing loss. The scope for reducing their energy consumption is significant and includes combining water heaters with appropriate solar devices. Water heaters, hot water storage tanks and packages of water heaters and solar devices should therefore be covered by energy labelling requirements.(3) Water heaters that are designed for using gaseous orliquid fuels predominantly (more than 50 %) produced from biomass have specific technical characteristics which require further technical, economic and environ mental analyses. Depending on the outcome of the analyses, energy labelling requirements for those water heaters should be set at a later stage, if appropriate.(4) Harmonised provisions should be laid down on labellingand standard product information regarding the energyefficiency of water heaters and hot water storage tanks in order to provide incentives for manufacturers to improve the energy efficiency of these products, to encourage end- users to purchase energy-efficient products and to contribute to the functioning of the internal market.(5) As regards significant energy and cost savings for eachtype of water heater and for hot water storage tanks, thisRegulation should introduce a new single labelling scalefrom A to G for conventional water heaters, solar water heaters and heat pump water heaters and for hot waterstorage tanks. A dynamic class A + should be added to theclassification after two years to accelerate the market penetration of the most efficient water heaters and hot water storage tanks.(6) ThisRegulation should ensure that consumers get more accurate comparative information about the performance of solar water heaters and heat pump water heaters in three European climate zones.(7) Thesound power level of a water heater could be an important consideration for end-users. Information on sound power levels should be included on the labels of water heaters.(8) Thecombined effect of this Regulation and Commission Regulation (EU) No 814/2013 of 2 August 2013 imple menting Directive 2009/125/EC of the European Parliament and of the Council with regard to ecodesign requirements for water heaters and hot water storagetanks ( 2 ) is expected to result in estimated annual energy savings of around 450 PJ (11 Mtoe) by 2020,corresponding to about 26 Mt CO 2emissions, compared to what would happen if no measures were taken.(9) Theinformation provided on the labels should be obtained through reliable, accurate and reproduciblemeasurement and calculation procedures that take into account recognised state-of-the-art measurement and calculation methods including, where available, harmonised standards adopted by the European standard isation bodies under a request from the Commission, in accordance with the procedures laid down in the Directive 98/34/EC of the European Parliament and of the Council of 22 June 1998 laying down a procedure for the provision of information in the field of technical standards and regulations and of rules on Information Society services ( 3 ), for the purpose of establishing ecodesign requirements.(10) This Regulation should specify a uniform design andcontent of product labels for water heaters and hotwater storage tanks.( 1 ) OJ L 153, 18.6.2010, p. 1.( 2 ) See page 162 of this Official Journal. ( 3 ) OJ L 204, 21.7.1998, p. 37.(11) In addition, this Regulation should specify requirementsfor the product fiche and technical documentation forwater heaters and hot water storage tanks.(12) Moreover, this Regulation should specify requirementsfor the information to be provided for any form ofdistance selling of water heaters and hot water storagetanks and in any advertisements and technicalpromotional material for such products.(13)In addition to the product labels and fiches for waterheaters and hot water storage tanks laid down in thisRegulation, a package label and fiche based on productfiches from suppliers should ensure that the end-user haseasy access to information on the energy performance ofwater heaters in combination with solar devices. Themost efficient class A+++may be reached by such apackage.(14) It is appropriate to provide for a review of the provisionsof this Regulation taking into account technologicalprogress,HAS ADOPTED THIS REGULATION:Article 1Subject matter and scope1. This Regulation establishes requirements for the energy labelling of, and the provision of supplementary product information on, water heaters with a rated heat output ≤70 kW, hot water storage tanks with a storage volume ≤ 500 litres and packages of water heater ≤ 70 kW and solar device.2. This Regulation shall not apply to:(a) water heaters specifically designed for using gaseous orliquid fuels predominantly produced from biomass;(b) water heaters using solid fuels;(c) water heaters within the scope of Directive 2010/75/EU ofthe European Parliament and of the Council (1);(d) combination heaters as defined in Article 2 of CommissionDelegated Regulation (EU) No 811/2013 (2);(e) water heaters which do not meet at least the load profilewith the smallest reference energy, as specified in Annex VII, Table 3;(f) water heaters designed for making hot drinks and/or foodonly.Article 2DefinitionsIn addition to the definitions set out in Article 2 of Directive 2010/30/EU, the following definitions shall apply for the purposes of this Regulation:(1) ‘water heater’ means a device that:(a) is connected to an external supply of drinking orsanitary water;(b) generates and transfers heat to deliver drinking orsanitary hot water at given temperature levels, quantities and flow rates during given intervals; and(c) is equipped with one or more heat generators;(2) ‘heat generator’ means the part of a water heater thatgenerates the heat using one or more of the following processes:(a) combustion of fossil fuels and/or biomass fuels;(b) use of the Joule effect in electric resistance heatingelements;(c) capture of ambient heat from an air source, watersource or ground source, and/or waste heat;(3) ‘rated heat output’ means the declared heat output of thewater heater when providing water heating at standard rating conditions, expressed in kW;(4) ‘storage volume’ (V) means the rated volume of a hotwater storage tank, expressed in litres;(5) ‘standard rating conditions’ means the operatingconditions of water heaters for establishing the rated heat output, water heating energy efficiency and sound power level, and of hot water storage tanks for establishing the standing loss;(6) ‘biomass’ means the biodegradable fraction of products,waste and residues from biological origin from agriculture (including vegetal and animal substances), forestry and related industries including fisheries and aquaculture, as well as the biodegradable fraction of industrial and municipal waste;(7) ‘biomass fuel’ means a gaseous or liquid fuel producedfrom biomass;(8) ‘fossil fuel’ means a gaseous or liquid fuel of fossil origin;(1) OJ L 334, 17.12.2010, p. 17.(2) See page 1 of this Official Journal.(9) ‘hot water storage tank’ means a vessel for storing hotwater for water and/or space heating purposes, includingany additives, which is not equipped with any heatgenerator except possibly one or more back-up immersion heaters;(10) ‘back-up immersion heater’ means a Joule effect electricresistance heater that is part of a hot water storage tankand generates heat only when the external heat source isdisrupted (including during maintenance periods) or out oforder, or that is part of a solar hot water storage tank andprovides heat when the solar heat source is not sufficientto satisfy required comfort levels;(11) ‘solar device’ means a solar-only system, a solar collector, asolar hot water storage tank or a pump in the collectorloop, which are placed on the market separately;(12) ‘solar-only system’ means a device that is equipped withone or more solar collectors and solar hot water storagetanks and possibly pumps in the collector loop and otherparts, which is placed on the market as one unit and is notequipped with any heat generator except possibly one ormore back-up immersion heaters;(13) ‘package of water heater and solar device’ means a packageoffered to the end-user containing one or more waterheaters and one or more solar devices;(14) ‘water heating energy efficiency’ (ηwh) means the ratiobetween the useful energy provided by a water heater ora package of water heater and solar device and the energyrequired for its generation, expressed in %;(15) ‘sound power level’ (L WA) means the A-weighted soundpower level, indoors and/or outdoors, expressed in dB;(16) ‘standing loss’ (S) means the heating power dissipated froma hot water storage tank at given water and ambienttemperatures, expressed in W;(17) ‘heat pump water heater’ means a water heater that usesambient heat from an air source, water source or groundsource, and/or waste heat for heat generation.For the purposes of Annexes II to IX, additional definitions are set out in Annex I.Article 3Responsibilities of suppliers and timetable1. From 26 September 2015 suppliers placing water heaters on the market and/or putting them into service, including those integrated in packages of water heater and solar device, shall ensure that: (a) a printed label complying with the format and content ofinformation set out in point 1.1 of Annex III is provided foreach water heater conforming to the water heating energy efficiency classes set out in point 1 of Annex II, whereby: for heat pump water heaters, the printed label is provided atleast in the packaging of the heat generator; for water heaters intended for use in packages of water heater and solar device, a second label complying with the format and content of information set out in point 3 of Annex III is provided for each water heater;(b) a product fiche, as set out in point 1 of Annex IV, isprovided for each water heater, whereby: for heat pump water heaters, the product fiche is provided at least for the heat generator; for water heaters intended for use in packages of water heater and solar device, a second fiche, as set out in point 4 of Annex IV, is provided;(c) the technical documentation, as set out in point 1 ofAnnex V, is provided on request to the authorities of the Member States and to the Commission;(d) any advertisement relating to a specific water heater modeland containing energy-related or price information includesa reference to the water heating energy efficiency classunder average climate conditions for that model;(e) any technical promotional material concerning a specificwater heater model and describing its specific technical parameters includes a reference to the water heating energy efficiency class under average climate conditions for that model.From 26 September 2017 a printed label complying with the format and content of information set out in point 1.2 of Annex III shall be provided for each water heater conforming to the water heating energy efficiency classes set out in point 1 of Annex II, whereby: for heat pump water heaters, the printed label shall be provided at least in the packaging of the heat generator.2. From 26 September 2015 suppliers placing hot water storage tanks on the market and/or putting them into service shall ensure that:(a) a printed label complying with the format and content ofinformation set out in point 2.1 of Annex III is provided foreach hot water storage tank conforming to the energy efficiency classes set out in point 2 of Annex II;(b) a product fiche, as set out in point 2 of Annex IV, isprovided;(c) the technical documentation, as set out in point 2 ofAnnex V, is provided on request to the authorities of the Member States and to the Commission;(d) any advertisement relating to a specific hot water storagetank model and containing energy-related or price information includes a reference to the energy efficiency class for that model;(e) any technical promotional material concerning a specifichot water storage tank model and describing its specific technical parameters includes a reference to the energy efficiency class for that model.From 26 September 2017 a printed label complying with the format and content of information as set out in point 2.2 of Annex III shall be provided for each hot water storage tank conforming to the energy efficiency classes set out in point 2 of Annex II.3. From 26 September 2015 suppliers placing solar devices on the market and/or putting them into service shall ensure that:(a) a product fiche, as set out in point 3 of Annex IV, isprovided;(b) the technical documentation, as set out in point 3 ofAnnex V, is provided on request to the authorities of the Member States and to the Commission.4. From 26 September 2015 suppliers placing packages of water heater and solar device on the market and/or putting them into service shall ensure that:(a) a printed label complying with the format and content ofinformation set out in point 3 of Annex III is provided for each package of water heater and solar device conforming to the water heating energy efficiency classes set out in point 1 of Annex II;(b) a product fiche, as set out in point 4 of Annex IV, isprovided for each package of water heater and solar device;(c) the technical documentation, as set out in point 4 ofAnnex V, is provided on request to the authorities of the Member States and to the Commission;(d) any advertisement relating to a specific package of waterheater and solar device model and containing energy- related or price information includes a reference to the water heating energy efficiency class under average climate conditions for that model;(e) any technical promotional material concerning a specificpackage of water heater and solar device model and describing its specific technical parameters includes areference to the water heating energy efficiency class under average climate conditions for that model.Article 4Responsibilities of dealers1. Dealers of water heaters shall ensure that:(a) each water heater, at the point of sale, bears the labelprovided by suppliers in accordance with Article 3(1), as set out in point 1 of Annex III, on the outside of the front of the appliance, in such a way as to be clearly visible;(b) water heaters offered for sale, hire or hire-purchase, wherethe end-user cannot be expected to see the water heater displayed, are marketed with the information provided by the suppliers in accordance with point 1 of Annex VI;(c) any advertisement relating to a specific water heater modeland containing energy-related or price information includesa reference to the water heating energy efficiency classunder average climate conditions for that model;(d) any technical promotional material concerning a specificwater heater model and describing its specific technical parameters includes a reference to the water heating energy efficiency class under average climate conditions for that model.2. Dealers of hot water storage tanks shall ensure that:(a) each hot water storage tank, at the point of sale, bears thelabel provided by suppliers in accordance with Article 3(2), as set out in point 2 of Annex III, on the outside of the front of the appliance, in such a way as to be clearly visible;(b) hot water storage tanks offered for sale, hire or hire-purchase, where the end-user cannot be expected to see the hot water storage tank displayed, are marketed with the information provided by the suppliers in accordance with point 2 of Annex VI;(c) any advertisement relating to a specific hot water storagetank model and containing energy-related or price information includes a reference to the energy efficiency class for that model;(d) any technical promotional material concerning a specifichot water storage tank model and describing its specific technical parameters includes a reference to the energy efficiency class for that model.3. Dealers of packages of water heater and solar device shall ensure, based on the label and fiches provided by suppliers in accordance with Article 3(1), (3) and (4), that:(a) any offer for a specific package includes the water heatingenergy efficiency and the water heating energy efficiency class for that package under average, colder or warmer climate conditions, as applicable, by displaying with the package the label set out in point 3 of Annex III and providing the fiche set out in point 4 of Annex IV, duly filled in according to the characteristics of that package;(b) packages of water heater and solar device offered for sale,hire or hire-purchase, where the end-user cannot be expected to see the package of water heater and solar device displayed, are marketed with the information provided in accordance with point 3 of Annex VI;(c) any advertisement relating to a specific package of waterheater and solar device model and containing energy- related or price information includes a reference to the water heating energy efficiency class under average climate conditions for that model;(d) any technical promotional material concerning a specificpackage of water heater and solar device model and describing its specific technical parameters includes a reference to the water heating energy efficiency class under average climate conditions for that model.Article 5Measurement and calculation methodsThe information to be provided pursuant to Articles 3 and 4 shall be obtained by reliable, accurate and reproducible measurement and calculation methods which take into account the recognised state-of-the-art measurement and calculation methods, as set out in Annex VII and Annex VIII.Article 6Verification procedure for market surveillance purposes Member States shall apply the procedure set out in Annex IX when assessing the conformity of the declared water heating energy efficiency class, water heating energy efficiency, annual energy consumption and sound power level of water heaters and the declared energy efficiency class and standing loss of hot water storage tanks.Article 7ReviewThe Commission shall review this Regulation in the light of technological progress no later than five years after its entry into force. The review shall in particular assess any significant changes in the market shares of various types of appliances and the appropriateness of the package fiche and label set out in point 3 of Annex III and point 4 of Annex IV.Article 8Entry into force and applicationThis Regulation shall enter into force on the twentieth day following that of its publication in the Official Journal of the European Union.This Regulation shall be binding in its entirety and directly applicable in all Member States. Done at Brussels, 18 February 2013.For the CommissionThe PresidentJosé Manuel BARROSOANNEX IDefinitions applicable for Annexes II to IXFor the purposes of Annexes II to IX, the following definitions shall apply:(1) ‘conventional water heater’ means a water heater that generates heat using the combustion of fossil and/or biomassfuels and/or the Joule effect in electric resistance heating elements;(2) ‘solar water heater’ means a water heater equipped with one or more solar collectors, solar hot water storage tanks,heat generators and possibly pumps in the collector loop and other parts, a solar water heater is placed on the market as one unit;(3) ‘load profile’ means a given sequence of water draw-offs, as specified in Annex VII, Table 3; each water heater meetsat least one load profile;(4) ‘water draw-off’ means a given combination of useful water flow rate, useful water temperature, useful energycontent and peak temperature, as specified in Annex VII, Table 3;(5) ‘useful water flow rate’ (f) means the minimum flow rate, expressed in litres per minute, for which hot water iscontributing to the reference energy, as specified in Annex VII, Table 3;(6) ‘useful water temperature’ (T m) means the water temperature, expressed in degrees Celsius, at which hot water startscontributing to the reference energy, as specified in Annex VII, Table 3;(7) ‘useful energy content’ (Q tap) means the energy content of hot water, expressed in kWh, provided at a temperatureequal to, or above, the useful water temperature, and at water flow rates equal to, or above, the useful water flow rate, as specified in Annex VII, Table 3;(8) ‘energy content of hot water’ means the product of the specific heat capacity of water, the average temperaturedifference between the hot water output and cold water input, and the total mass of the hot water delivered;(9) ‘peak temperature’ (T p) means the minimum water temperature, expressed in degrees Celsius, to be achieved duringwater draw-off, as specified in Annex VII, Table 3;(10) ‘reference energy’ (Q ref) means the sum of the useful energy content of water draw-offs, expressed in kWh, in aparticular load profile, as specified in Annex VII, Table 3;(11) ‘maximum load profile’ means the load profile with the greatest reference energy that a water heater is able toprovide while fulfilling the temperature and flow rate conditions of that load profile;(12) ‘declared load profile’ means the load profile applied when determining water heating energy efficiency;(13) ‘conversion coefficient’ (CC) means a coefficient reflecting the estimated 40 % average EU generation efficiencyreferred to in Directive 2012/27/EU of the European Parliament and of the Council (1); the value of the conversion coefficient is CC = 2,5;(14) ‘daily electricity consumption’ (Q elec) means the consumption of electricity over 24 consecutive hours under thedeclared load profile and under given climate conditions, expressed in kWh in terms of final energy;(15) ‘daily fuel consumption’ (Q fuel) means the consumption of fuels over 24 consecutive hours under the declared loadprofile and under given climate conditions, expressed in kWh in terms of GCV, and for the purposes of point 4 in Annex VIII expressed in GJ in terms of GCV;(16) ‘gross calorific value’ (GCV) means the total amount of heat released by a unit quantity of fuel when it is burnedcompletely with oxygen and when the products of combustion are returned to ambient temperature; this quantity includes the condensation heat of any water vapour contained in the fuel and of the water vapour formed by the combustion of any hydrogen contained in the fuel;(17) ‘smart control’ means a device that automatically adapts the water heating process to individual usage conditionswith the aim of reducing energy consumption;(1) OJ L 315, 14.11.2012, p. 1.(18) ‘smart control compliance’ (smart) means the measure of whether a water heater equipped with smart controls fulfilsthe criterion set out in point 5 of Annex VIII;(19) ‘smart control factor’ (SCF) means the water heating energy efficiency gain due to smart control under the conditionsset out in point 3 of Annex VII;(20) ‘weekly electricity consumption with smart controls’ (Q elec,week,smart) means the weekly electricity consumption of awater heater with the smart control function enabled, expressed in kWh in terms of final energy;(21) ‘weekly fuel consumption with smart controls’ (Q fuel,week,smart) means the weekly fuel consumption of a water heaterwith the smart control function enabled, expressed in kWh in terms of GCV;(22) ‘weekly electricity consumption without smart controls’ (Q elec,week) means the weekly electricity consumption of awater heater with the smart control function disabled, expressed in kWh in terms of final energy;(23) ‘weekly fuel consumption without smart controls’ (Q fuel,week) means the weekly fuel consumption of a water heaterwith the smart control function disabled, expressed in kWh in terms of GCV;(24) ‘annual electricity consumption’ (AEC) means the annual electricity consumption of a water heater under thedeclared load profile and under given climate conditions, expressed in kWh in terms of final energy;(25) ‘annual fuel consumption’ (AFC) means the annual fossil and/or biomass fuel consumption of a water heater underthe declared load profile and under given climate conditions, expressed in GJ in terms of GCV;(26) ‘ambient correction term’ (Q cor) means a term which takes into account the fact that the place where the waterheater is installed is not an isothermal place, expressed in kWh;(27) ‘standby heat loss’ (P stby) means the heat loss of a heat pump water heater in operating modes without heat demand,expressed in kW;(28) ‘average climate conditions’, ‘colder climate conditions’ and ‘warmer climate conditions’ mean the temperatures andglobal solar irradiance conditions characteristic for the cities of Strasbourg, Helsinki and Athens, respectively;(29) ‘annual energy consumption’ (Q tota) means the annual energy consumption of a solar water heater, expressed in kWhin terms of primary energy and/or kWh in terms of GCV;(30) ‘annual non-solar heat contribution’ (Q nonsol), means the annual contribution of electricity (expressed in kWh interms of primary energy) and/or fuels (expressed in kWh in terms of GCV) to the useful heat output of a solar water heater or a package of water heater and solar device, taking into account the annual amount of heat captured by the solar collector and the heat losses of the solar hot water storage tank;(31) ‘solar collector’ means a device designed to absorb global solar irradiance and to transfer the heat energy soproduced to a fluid passing through it; it is characterised by the collector aperture area, the zero-loss efficiency, the first order coefficient, the second-order coefficient and the incidence angle modifier;(32) ‘global solar irradiance’ means the rate of total incoming solar energy, both direct and diffuse, on a collector planewith an inclination of 45 degrees and southward orientation at the Earth’s surface, expressed in W/m2;(33) ‘collector aperture area’ (A sol) means the maximum projected area through which unconcentrated solar radiationenters the collector, expressed in m2;(34) ‘zero-loss efficiency’ (η0) means the efficiency of the solar collector, when the solar collector mean fluid temperatureis equal to the ambient temperature;(35) ‘first-order coefficient’ (a1) means the heat loss coefficient of a solar collector, expressed in W/(m2K);(36) ‘second-order coefficient’ (a2) means the coefficient measuring the temperature dependence of the first order coefficient, expressed in W/(m2K2);(37) ‘incidence angle modifier’ (IAM) means the ratio of the useful heat output of the solar collector at a given incidenceangle and its useful heat output at an incidence angle of 0 degrees;。
第45卷㊀第6期2021年11月南京林业大学学报(自然科学版)JournalofNanjingForestryUniversity(NaturalScienceEdition)Vol.45,No.6Nov.,2021‘南京林业大学学报(自然科学版)“2021年论文题录(作者)索引AUTHORANDSUBJECTINDEXES2021著录格式:作者.文题(外文文题).刊名,出版年,卷(期):起止页码.(作者以姓氏汉语拼音为序)白文玉,冯茂松,铁烈华,汪亚琳,高嘉翔,赖㊀娟,戴晓康.不同无性系四川桤木嫁接苗生物量及其分配特征(Biomassanditsallocationcharacteristicsofone⁃year⁃oldgraftedseedlingsofdifferentclonesofAlnusledgeriana).南京林业大学学报(自然科学版),2021,45(2):87-95.蔡龙涛,邢㊀涛,邢艳秋,丁建华,黄佳鹏,崔㊀阳,秦㊀磊.基于ICESat⁃GLAS数据和模糊模式识别算法识别森林类型(IdentificationofforesttypesbasedonICESat⁃GLASdataandfuzzypatternrecognitionalgorithm).南京林业大学学报(自然科学版),2021,45(4):33-41.曹桥荣,董京京,钟文峰,彭智奇,董㊀鹏,陈㊀洁,伊贤贵.樱花新品种 胭脂绯 (Cerasusdielsiana Yanzhifei :anewcultivaroforientalcher⁃ry).南京林业大学学报(自然科学版),2021,45(1):242-244.陈㊀佳,缑晶毅,赵㊀祺,韩庆庆,李慧萍,姚㊀丹,张金林.梭梭根际根瘤菌对紫花苜蓿生长及耐盐性的影响(InducedgrowthandsalttoleranceofalfalfabyrhizobiumstrainsfromtherhizosphereofHaloxylonammodendron).南京林业大学学报(自然科学版),2021,45(6):99-110.陈㊀黎,刘成功,钱莹莹,唐晓蝶,王生树,李志东,李㊀燕,崔㊀珺.南方红豆杉人工林针叶C㊁N㊁P化学计量特征(StoichiometriccharacteristicsofC,N,PofTaxuschinensisvar.maireiplantationneedles).南京林业大学学报(自然科学版),2021,45(5):53-61.陈㊀林,潘婷婷,吕笑冬,汪章沛,程㊀林.江西省种子植物分布新资料(NewrecordsofseedplantsfromJiangxiProvince).南京林业大学学报(自然科学版),2021,45(5):232-234.陈宏健,郝德君,田㊀敏,周㊀杨,夏小洪,赵欣怡,乔㊀恒,谈家金.室内饲养松墨天牛幼虫不同肠段细菌的群落结构及功能分析(Thecom⁃munitystructureandfunctionalanalysisofintestinalbacteriainMonochamusalternatuslarvaerearedindoors).南京林业大学学报(自然科学版),2021,45(3):143-151.陈隆升,梅㊀莉,陈永忠,赵泽尧,许彦明,张㊀震,胡亚军,刘彩霞,苏以荣.油茶林生草栽培对地表径流及氮磷流失特征的影响(EffectsofinterplantingherbageonsurfacerunoffassociatedwithnitrogenandphosphoruslossesinCamelliaoleiferaplantations).南京林业大学学报(自然科学版),2021,45(6):127-134.陈兴彬,徐海宁,肖复明,孙世武,娄永峰,邹元熹,徐小强.陈山红心杉1.5代种子园遗传多样性和子代父本分析(Geneticdiversityandpaternityanalysesina1.5thgenerationseedorchardofChenshanred⁃heartChinesefir).南京林业大学学报(自然科学版),2021,45(3):87-92.陈秀波,段文标,陈立新,朱德全,赵晨晨,刘东旭.小兴安岭3种原始红松混交林土壤nirK型反硝化微生物群落特征(CommunitystructureanddiversityofsoilnirK⁃typedenitrifyingmicroorganismsinthreeforesttypesofprimitivePinuskoraiensismixedforestinLiangshuiNationalNatureReserve,LesserKhinganMountains).南京林业大学学报(自然科学版),2021,45(2):77-86.陈玉华,姚㊀丹,吴海楠,陶申童,吴吉妍,杨文国,童春发.美洲黑杨与小叶杨杂交F1代扦插无性系苗生长性状动态分析(AnalysesofdynamicgrowthtraitsofthestecklingsfromtheF1hybridprogenyofPopulusdeltoidesˑP.simonii).南京林业大学学报(自然科学版),2021,45(1):45-52.程㊀娟,丁访军,谭正洪,廖立国,周㊀汀,崔迎春.贵州茂兰喀斯特森林两树种叶片气孔形态特征及其对蒸腾的影响(Leafstomatalmorpho⁃logicalcharacteristicsandtheireffectsontranspirationfortwotreespeciesinMaolanKarstarea,GuizhouProvince).南京林业大学学报(自然科学版),2021,45(5):125-132.崔㊀阳,狄海廷,邢艳秋,常晓晴,单㊀炜.基于MODIS数据的2001 2018年黑龙江省林火时空分布(SpatialandtemporaldistributionsofforestfiresinHeilongjiangProvincefrom2001to2018basedonMODISdata).南京林业大学学报(自然科学版),2021,45(1):205-211.崔令军,刘瑜霞,林㊀健,石开明.丛枝菌根真菌对盐胁迫下桢楠光合生理的影响(EffectsofAMFonphotosyntheticcharacteristicsofPhoebezhennanundersaltstress).南京林业大学学报(自然科学版),2021,45(1):101-106.邓㊀平,赵㊀英,王㊀霞,陈秋佑,吴㊀敏.水杨酸对NaHCO3胁迫下桂西北喀斯特地区青冈栎种子萌发的影响(Effectsofsalicylicacidonger⁃minationofCyclobalanopsisglaucaseedsunderNaHCO3stressinKarstareaofnorthwestGuangxi).南京林业大学学报(自然科学版),2021,45. All Rights Reserved.南京林业大学学报(自然科学版)第45卷(4):114-122.邓㊀睿,张梅丽,周㊀明,郑宝江.中国茶藨子属1新记录种(AnewlyrecordedplantofthegenusRibesfromChina).南京林业大学学报(自然科学版),2021,45(2):231-233.丁㊀胜,李㊀颂,梁钰坤,赵庆建,曹福亮,吕㊀柳.经济林产业上市公司融资影响因素分析(Factorsinfluencingthefinancingoflistedcompaniesinnon⁃timberforestindustry).南京林业大学学报(自然科学版),2021,45(3):224-232.董灵波,刘兆刚.森林健康评价及其多尺度转换方法(Foresthealthassessmentsandmulti⁃scaleconversionmethods).南京林业大学学报(自然科学版),2021,45(3):206-216.杜晋城,李欣欣,邓小兵,慕长龙.9个油橄榄品种叶片功能性状特征比较(Comparisonsofleaffunctionalcharacteristicsofnineolivevarieties).南京林业大学学报(自然科学版),2021,45(2):159-164.段一凡,李㊀岚,杨欣欣,王贤荣,张㊀敏,张㊀成,柴子涵.桂花及其近缘种倍性和基因组大小分析(StudyonploidyandgenomesizesofOs⁃manthusfragransanditsrelatedspecies).南京林业大学学报(自然科学版),2021,45(5):47-52.范佳辉,张亚丽,李明诗.基于空间光谱信息协同的城市不透水层提取方法比较研究(Comparingfourmethodsforextractingimpervioussurfacesusingspectralinformationinsynergywithspatialheterogeneityofremotelysensedimagery).南京林业大学学报(自然科学版),2021,45(1):212-218.费宜玲,侯森林,唐松泽.近三年江苏省非法被猎两栖爬行动物分析(AnalysisofillegalhuntingcasesofamphibiansandreptilesinJiangsuProvinceoverthepastthreeyears).南京林业大学学报(自然科学版),2021,45(2):225-230.冯园园,李清莹,黄均华,胡绍庆.25个彩叶桂无性系(品种)的数量分类研究(Numericalclassificationof25color⁃leafedOsmanthusfragransclones(cultivars)).南京林业大学学报(自然科学版),2021,45(1):107-115.高㊀芳,陈士刚,秦彩云,才巨锋,王聪慧,董环宇,陶㊀晶.红皮云杉体胚发生体系优化和超低温保存技术研究(OptimizationofsomaticembryogenesissystemandcryopreservationofPiceakoraiensis).南京林业大学学报(自然科学版),2021,45(3):100-108.高㊀燕,莫建彬,付艳茹,奉树成.铁线莲 朱卡 组织培养技术及再生体系的建立(TissuecultureandplantregenerationofClematis Julka ).南京林业大学学报(自然科学版),2021,45(3):109-114.高景斌,徐六一,叶建仁.马尾松松材线虫病抗性无性系的筛选和遗传多样性分析(GrowthandgeneticdiversityanalysisofclonesscreenedbyphenotypicalresistanttopinewiltdiseaseinPinusmassoniana).南京林业大学学报(自然科学版),2021,45(5):109-118.高沁怡,潘春霞,刘㊀强,顾光同,祝雅璐,吴伟光.基于贝叶斯网络的林业碳汇项目风险评价(RiskassessmentsofforestrycarbonsequestrationprojectsbasedonBayesiannetwork).南京林业大学学报(自然科学版),2021,45(4):210-218.葛宝柱,徐㊀强,陈赢男.山新杨蔗糖合酶基因PCR介导的重组现象研究(ThephenomenonofPCR⁃mediatedrecombinationbyusingSUSgenesofPopulusdavidianaˑP.bolleana).南京林业大学学报(自然科学版),2021,45(3):79-86.郭㊀亮,丁九敏,徐㊀侠.树干甲烷的研究进展(Advancesinresearchonmethanefromtreestems).南京林业大学学报(自然科学版),2021,45(5):235-241.郭㊀雯,漆良华,雷㊀刚,胡㊀璇,张㊀建,舒㊀琪,商泽安.毛竹及其变种叶片化学计量与养分重吸收效率(Leafstoichiometryandnutrientre⁃absorptionefficiencyofPhyllostachysedulisanditsvarieties).南京林业大学学报(自然科学版),2021,45(1):79-85.郭佳惠,教忠意,何旭东,诸葛强,周㊀洁.基于层次分析法对柳树观赏性及适应性的综合评价(Acomprehensiveevaluationofornamentalcharacteristicsandadaptabilityofwillowsbasedonanalytichierarchyprocesses).南京林业大学学报(自然科学版),2021,45(6):169-176.郭天威,陆春锋,王君櫹,刘瑞程,周生路.基于三生空间耦合的生态安全格局构建与优化 以扬州市为例(Constructionandoptimizationofecologicalsecuritypatternbasedonthecouplingofecological⁃production⁃livingspaces:takingYangzhouCityasanexample).南京林业大学学报(自然科学版),2021,45(5):133-142.何旭东,郑纪伟,孙㊀冲,何开跃,王保松.33个杨柳品种指纹图谱构建(Constructionoffingerprintsfor33varietiesinSalicaceae).南京林业大学学报(自然科学版),2021,45(2):35-42.贺梦莹,董利虎,李凤日.长白落叶松-水曲柳混交林不同混交方式单木冠长预测模型(TreecrownlengthpredictionmodelsforLarixolgensisandFraxinusmandshuricainmixedplantationswithdifferentmixingmethods).南京林业大学学报(自然科学版),2021,45(4):13-22.洪㊀震,刘术新,洪琮浩,雷小华.5种造林树种对干旱胁迫的抗性应答(Resistanceresponseoffiveafforestationtreespeciesunderdroughtstress).南京林业大学学报(自然科学版),2021,45(2):111-119.侯㊀静,毛金燕,翟㊀惠,王㊀洁,尹佟明.CRISPR/Cas技术在木本植物改良中的应用(ApplicationofCRISPR/Castechniqueinwoodyplantimprovement).南京林业大学学报(自然科学版),2021,45(6):24-30.胡丁猛,许景伟,王立辉,囤兴建,朱升祥,杨㊀健. 蕊沁 等7个海棠新品种(Sevennewcrabapplecultivarssuchas RuiQin ).南京林业大学学报(自然科学版),2021,45(4):238-242.胡海波,贾西川.我国平原农区林带胁地效应及其控制措施研究进展(Reviewonnegativeeffectsanditscontrolmeasuresofforestbeltinplain242. All Rights Reserved.㊀第6期‘南京林业大学学报(自然科学版)“2021年论文题录(作者)索引agriculturalareasofChina).南京林业大学学报(自然科学版),2021,45(2):234-240.花伟成,田佳榕,孙心雨,徐雁南.基于TLS数据的杨树削度方程建立及材积估算(Assessingthestemtaperfunctionandvolumeestimationofpoplar(Populus)byterrestriallaserscanning).南京林业大学学报(自然科学版),2021,45(4):41-48.惠㊀昊,关庆伟,王亚茹,林鑫宇,陈㊀斌,王㊀刚,胡㊀月,胡敬东.不同森林经营模式对土壤氮含量及酶活性的影响(Effectsofdifferentforestmanagementmodesonsoilnitrogencontentandenzymeactivity).南京林业大学学报(自然科学版),2021,45(4):151-158.火㊀艳,张慧会,祝遵凌.欧洲鹅耳枥水培营养液适用效果比较研究(ComparativestudyontheeffectsofnutrientsolutiontreatmentinhydroponiccultivationofCarpinusbetulus).南京林业大学学报(自然科学版),2021,45(2):17-24.嵇保中,张㊀磊,刘曙雯,姜宏健,金明霞.白蚁品级分化研究进展(Researchprogressoftermitecastedifferentiation).南京林业大学学报(自然科学版),2021,45(5):1-9.季㊀淮,韩建刚,李萍萍,朱咏莉,郭俨辉,郝达平,崔㊀皓.洪泽湖湿地植被类型对土壤有机碳粒径分布及微生物群落结构特征的影响(EffectsofdifferentvegetationtypesonsoilorganiccarbonparticlesizedistributionandmicrobialcommunitystructureinHongzeLakeWetland).南京林业大学学报(自然科学版),2021,45(1):141-150.季艳红,汤文华,窦全琴,谢寅峰.施肥对薄壳山核桃容器苗生长及养分积累的影响(EffectsoffertilizerapplicationonseedlinggrowthandnutrientaccumulationinCaryaillinoinensiscontainerseedlings).南京林业大学学报(自然科学版),2021,45(6):47-56.贾㊀婷,宋武云,关新贤,魏智文,陈㊀涵,易㊀敏,熊启慧,张㊀露.湿地松针叶功能性状及其对磷添加的响应(ResponsesofneedlefunctionaltraitsofPinuselliottiitophosphorusaddition).南京林业大学学报(自然科学版),2021,45(6):65-71.江杏香,陈玉凯,吴石松,陈㊀庆.海南濒危植物蕉木种群结构与动态特征(PopulationstructureanddynamicsoftheendangeredplantChienio⁃dendronhainanenseinHainan).南京林业大学学报(自然科学版),2021,45(1):116-122.蒋㊀瑶,魏海林,高昌虎,王大故,冯楠可,李㊀睿,刘榕榕,吕芳德.湖南低山丘陵区薄壳山核桃的开花物候期观测及品种配置(ObservationonfloweringphenologyandvarietycombinationofCaryaillinoinensisinlowmountainsandhillsofHunanProvince).南京林业大学学报(自然科学版),2021,45(1):53-62.荆㊀烁,孙慧珍.东北东部山区主要树种枝条及其组分水力特征(ThehydrauliccharacteristicsofthewholebranchanditscomponentsofthemajortreespeciesintheeasternregionofnortheastChina).南京林业大学学报(自然科学版),2021,45(4):159-166.赖国桢,汪雁楠,黄宝祥,周㊀光,莫晓勇,刘丽婷.林分空间结构优化栅格间伐模型(Agridthinningmodelbasedonforestspatialstructureopti⁃mization).南京林业大学学报(自然科学版),2021,45(3):199-205.黎梦娟,朱礼明,霍俊男,张景波,施季森,成铁龙.唐古特白刺NtCBL1㊁NtCBL2基因克隆及表达分析(CloningandexpressionanalysesofNtCBL1,NtCBL2geneofNitrariatangutorum).南京林业大学学报(自然科学版),2021,45(3):93-99.李㊀磊,蒋㊀敬,陈云霞.DNA宏条形码技术在动植物法医鉴定中的应用进展(RecentadvancesintheapplicationofDNAmetabarcodingtech⁃nologyinforensicidentificationofanimalsandplants).南京林业大学学报(自然科学版),2021,45(1):235-241.李㊀蒙,董京京,丁明贵,王贤荣,伊贤贵.樱花新品种 名贵红 (Cerasusserrulatavar.lannesiana MingguiHong :anewcultivarofcherry).南京林业大学学报(自然科学版),2021,45(6):239-240.李㊀娜,朱培林,丰㊀采,温敏学,方升佐,尚旭岚.青钱柳嫁接愈合过程中砧穗生理特性及其与亲和性的关系(Variationsinphysiologicalcharacteristicsofrootstock⁃scionanditsrelationshiptograftcompatibilityduringthegraftingunionprocessofCyclocaryapaliurus).南京林业大学学报(自然科学版),2021,45(1):13-20.李㊀陶,李明阳,钱春花.结合冠层密度的森林净初级生产力遥感估测(Combiningcrowndensitytoestimateforestnetprimaryproductivitybyusingremotesensingdata).南京林业大学学报(自然科学版),2021,45(5):153-160.李㊀鑫,翁卫松,李明诗.太平洋西北部地区天然林景观动态及破碎化驱动力分析(AssessingnaturalforestfragmentationprocessdynamicsanditsdriversinthePacificnorthwestregion,USA).南京林业大学学报(自然科学版),2021,45(3):174-182.李佳琦,薛晓明,高捍东.桢楠种子脱水过程中的生理响应(PhysiologicalresponsesofPhoebezhennanseedsduringdehydration).南京林业大学学报(自然科学版),2021,45(3):130-136.李江荣,郭其强,郑维列.西藏东南部急尖长苞冷杉蒸腾耗水及其驱动因子分析(AnalysesoftranspirationwaterconsumptionofAbiesgeorgeivar.smithiianditsdrivingfactorsinsoutheasternTibet).南京林业大学学报(自然科学版),2021,45(6):151-158.李沁宇,刘㊀鑫,张金池.长三角区域酸雨类型转变趋势研究(ChangingtrendsofacidraintypesintheYangtzeRiverDeltaregion).南京林业大学学报(自然科学版),2021,45(1):168-174.李清顺,金万洲,王得军,孙景梅,李宏韬.利用多树种立地形指数对林地质量进行综合评价(Acomprehensiveevaluationofforestlandqualityusingmultitreesiteformindex).南京林业大学学报(自然科学版),2021,45(6):81-89.李庆杨,王树凤,吴书天,王若辉,施㊀翔,莫润宏,刘毅华.弗吉尼亚栎种子化学成分特征及其对立地土壤条件的响应(ChemicalcompositionsofQuercusvirginianaseedsandtheirresponsestosoilproperty).南京林业大学学报(自然科学版),2021,45(6):111-118.廖逸宁,郭素娟,王芳芳,马雅莉,刘亚斌.有机-无机肥配施对板栗园土壤肥力及根系功能性状的影响(Effectsofcombinedapplicationoforganicandinorganicfertilizersonsoilfertilityandrootfunctionaltraitsinchestnutorchards).南京林业大学学报(自然科学版),2021,45(5):84-92.林远锋,鲁长虎,许㊀鹏,崔㊀鹏,张文文.1976年以来丹顶鹤在我国的分布变迁及就地保护状况(Distributionchangesandinsituconservationofred⁃crownedcranes(Grusjaponensis)inChinasince1976).南京林业大学学报(自然科学版),2021,45(6):200-208.刘㊀浩,刘㊀璨,刘俊昌.中国退耕还林工程对农户收入和消费不平等的影响测度(Effectsoftheslopinglandconversionprogramimpactonthe342. All Rights Reserved.南京林业大学学报(自然科学版)第45卷ruralhouseholds incomeandconsumptioninequalitiesinChina).南京林业大学学报(自然科学版),2021,45(1):227-234.刘㊀楠,冯富娟,张秀月.原始红松林皆伐后穿透雨对凋落物淋溶过程的影响(Effectsofthelitterleachingprocessbythroughfallafterclearcut⁃tingofprimaryPinuskoraiensisforest).南京林业大学学报(自然科学版),2021,45(1):159-167.刘桂丰,张姝慧,李慧玉,姜㊀静,陈㊀肃,黄海娇,李长海.彩叶桦 紫霞1号 新品种(Betulapendula Zixia1 :anewbirchcultivar).南京林业大学学报(自然科学版),2021,45(5):245-246.刘俊涛,仲㊀静,刘济铭,罗水晶,王冕之,范嘉霖,贾黎明.无患子初果期人工林土壤和叶片C㊁N㊁P化学计量特征(StoichiometriccharacteristicsofsoilandleavesinSapindusmukorossiplantationatanearlyfruitingstage).南京林业大学学报(自然科学版),2021,45(4):67-75.刘亚静,周㊀来,张㊀博,陈丽萍,潘㊀磊,孙玉军.不同林龄杉木径向变化及其对气象因子的响应(RadialvariationofCunninghamialanceolataindifferentagedforestsanditsresponsetometeorologicalfactors).南京林业大学学报(自然科学版),2021,45(2):135-144.卢㊀军,刘宪钊,孟维亮,李红军.基于地面激光点云数据的单木三维重建方法(Methodologyofindividualtree3Dreconstructionbasedonterres⁃triallaserscanningpointclouddata).南京林业大学学报(自然科学版),2021,45(6):193-199.陆元昌,雷相东,王㊀宏,刘宪钊,孟京辉,谢阳生,国㊀红,姜㊀俊,高文强.森林作业法的历史发展与面向我国森林经营规划的三级作业法体系(Silviculturalsystem:thehistoricalreviewandnewdevelopmentofaHierarchicallyStructuredSystemformanagementplanninginChina).南京林业大学学报(自然科学版),2021,45(3):1-7.路买林,陈梦娇,张嘉嘉,赵建霞,朱景乐,杜红岩. 红叶 杜仲叶色转变过程中叶片生理指标变化(LeafphysiologicalindicatorchangesinthetransformationofleavescolorofEucommiaulmoides Hongye ).南京林业大学学报(自然科学版),2021,45(1):86-92.罗凤敏,高君亮,辛智鸣,郝玉光,李新乐,段瑞兵.乌兰布和沙漠绿洲防护林体系小气候效应研究(StudyonthemicroclimateeffectsofoasisshelterforestintheUlanBuhDesert).南京林业大学学报(自然科学版),2021,45(5):143-152.马丹丹,库伟鹏,夏国华,毛洁莹,薛建辉.珍稀濒危植物堇叶紫金牛种群结构及动态分析(StructureanddynamicsofrareandendangeredplantArdisiaviolaceanaturalpopulation).南京林业大学学报(自然科学版),2021,45(3):159-164.马秋月,李倩中,李淑顺,朱㊀璐,颜坤元,李淑娴,张㊀斌,闻㊀婧.元宝枫组织培养及快速繁殖技术研究(StudyontissuecultureandrapidpropagationofAcertruncatumBunge).南京林业大学学报(自然科学版),2021,45(2):220-224.马晓乾,葛㊀君,王㊀琪,赵红盈,孙㊀妍,高㊀宇,申国涛,于文喜.光肩星天牛对糖槭挥发物的EAG及嗅觉行为反应(EAGandolfactorybe⁃havioralresponsesofAnoplophoraglabripennis(Coleoptera:Cerambycidae)tovolatilesofAcersaccharum).南京林业大学学报(自然科学版),2021,45(1):123-130.马颖忆,刘志峰.江苏省景观生态风险评估及其与城镇化的动态响应(Assessmentoflandscapeecologicalriskanditsdynamicresponsewithur⁃banizationdevelopmentofJiangsuProvince).南京林业大学学报(自然科学版),2021,45(5):185-194.马永春,佘诚棋,方升佐.不同修枝方法对杨树人工林生长㊁光合叶面积和主干饱满度的影响(Effectsofpruningmethodsongrowth,photosyn⁃theticleafareaandplumpnessoftrunksegmentinpoplarplantations).南京林业大学学报(自然科学版),2021,45(4):137-142.蒙海勤,叶建仁,王旻嘉,曹伊扬.木腐真菌对松材线虫病疫木处理初探(Usingwoodrotfungitotreatplaguewoodcausedbypinewiltdisease).南京林业大学学报(自然科学版),2021,45(4):183-189.缪㊀菁,王㊀勇,王㊀璐,许晓岗.基于MaxEnt模型的苦槠潜在地理分布格局变迁预测(PredictionofpotentialgeographicaldistributionpatternchangeforCastanopsissclerophyllaonMaxEnt).南京林业大学学报(自然科学版),2021,45(3):193-198.倪㊀铭,高振洲,吴㊀文,张于卉,喻方圆.不同氮素施肥方法对纳塔栎容器苗生长及非结构性碳水化合物积累的影响(Effectsofdifferentni⁃trogenfertilizationmethodsongrowthandnon⁃structurecarbohydrateaccumulationofQuercusnuttalliiseedlings).南京林业大学学报(自然科学版),2021,45(4):107-113.彭智奇,董㊀鹏,朱㊀弘,朱淑霞,董京京,钟育谦,翟飞飞,郑爱春,王贤荣,伊贤贵.江苏云台山山樱花种群结构及点格局分析(AnalysesofCerasusserrulatapopulationstructureandpointpatternsinYuntaiMountain,Jiangsu).南京林业大学学报(自然科学版),2021,45(4):167-176.任世奇,朱原立,梁燕芳,陈健波,卢翠香,伍㊀琪,韦振道.基于PM模型的广西南宁尾巨桉中龄林蒸散特征(Evapotranspirationcharacteristicsofmiddle⁃agedEucalyptusurophyllaˑE.grandisplantationbasedonPenman⁃MonteithmodelinNanning,Guangxi).南京林业大学学报(自然科学版),2021,45(2):127-134.尚军烨,徐炜超,孟庆繁,赵红蕊,刘生冬,李㊀燕.栎丽虎天牛成虫触角感器的扫描电镜观察(AntennalsensillaofadultPlagionotuspulcher(Coleoptera:Cerambycidae)observedwithscanningelectronmicroscope).南京林业大学学报(自然科学版),2021,45(5):195-200.佘建炜,张㊀康,郑㊀旭,赵小军,程㊀方,唐罗忠.海水处理对沼泽小叶桦苗木生长和生理的影响(EffectsofseawaterongrowthandphysiologyofBetulamicrophyllavar.paludosacuttingseedlings).南京林业大学学报(自然科学版),2021,45(5):102-108.圣倩倩,戴安琪,宋㊀敏,唐㊀睿,祝遵凌.NO2胁迫下两种鹅耳枥的光合生理特性变化(PhotosyntheticphysiologicalcharacteristicsoftwokindsofhornbeamunderNO2stress).南京林业大学学报(自然科学版),2021,45(2):10-16.盛后财,姚月锋,蔡体久,郭㊀娜,琚存勇.物候变化对落叶松人工林降雨分配过程中钾和钠离子迁移的影响(Effectsofphenoseasonontransferofpotassiumandsodiumionsintheprocessofrainfallredistributioninlarch(Larixgmelinii)plantations).南京林业大学学报(自然科学版),2021,45(6):143-150.施季森.CRISPR:从 盲盒 基因编辑到 精准靶向 基因组编辑的未竟之旅(CIRSPR:aunfulfilledjourneyfromgeneeditingin Blindbox to Precisiontargeting genomeediting).南京林业大学学报(自然科学版),2021,45(6):12-14.石文广,李㊀靖,张玉红,雷静品,罗志斌.7种杨树铅抗性和积累能力的比较研究(Acomparativestudyonleadtoleranceandaccumulationof442. All Rights Reserved.㊀第6期‘南京林业大学学报(自然科学版)“2021年论文题录(作者)索引sevenpoplarspecies).南京林业大学学报(自然科学版),2021,45(3):61-70.石小庆,刘晓莉,李方文,朱章顺,马㊀娇,杨苑钊,曾心美.木芙蓉新品种 百日华彩 ( Bairihuacai :anewHibiscusmutabiliscultivar).南京林业大学学报(自然科学版),2021,45(5):242-244.石欣隆,杨月琴,薛㊀娴,刘㊀伟,宋程威,郭丽丽,侯小改.壳寡糖对干旱胁迫下 凤丹 幼苗生长及生理特性的影响(EffectsofchitooligosaccharideonthegrowthphysiologyofPaeoniaostii FengDan seedlingsunderdroughtstress).南京林业大学学报(自然科学版),2021,45(2):120-126.时㊀珍,邢露华,郑琳琳,穆㊀博,田国行.城市公园绿地游憩供需协同度评价及优化策略(Evaluationsandoptimizationstrategiesofsynergyde⁃greeofparkgreenspacebasedonbalanceofsupplyanddemandforrecreation).南京林业大学学报(自然科学版),2021,45(2):197-204.宋㊀爽,许大为,石梦溪,胡珊珊.挠力河流域景观生态健康时空演变(SpatialandtemporalevolutionoflandscapeecologicalhealthinNaoliheBasin).南京林业大学学报(自然科学版),2021,45(2):177-186.宋烨,彭红军,孙铭君.碳限额与交易下木质林产品供应链内部融资机制(Internalfinancingmechanismsofwoodforestproductsupplychainunderthecap⁃and⁃tradescheme).南京林业大学学报(自然科学版),2021,45(6):232-238.苏胜荣,王继山,刘腾腾,王恩翠,桑旦次仁,张小鹏,李昕宇,张天星.一种西藏藏川杨潜叶新害虫 柳潜细蛾(Areportonanewleaf⁃miningpestofPopulusszechuanicavar.tibeticainTibet:Phyllonoryoleypastorella).南京林业大学学报(自然科学版),2021,45(4):243-246.孙㊀开,江建平,丁雨龙,RAMAKRISHNAUMuthusamy,魏㊀强.毛竹竹秆秆柄形态与解剖学研究(MorphologicalandanatomicalanalysesofMosobambooculmnecks).南京林业大学学报(自然科学版),2021,45(6):40-46.孙㊀龙,窦㊀旭,胡同欣.林火对森林生态系统碳氮磷生态化学计量特征影响研究进展(ResearchprogressontheeffectsofforestfireonforestecosystemC⁃N⁃Pecologicalstoichiometrycharacteristics).南京林业大学学报(自然科学版),2021,45(2):1-9.孙海燕,李㊀强,朱铭玮,李永荣,李淑娴.油用牡丹 凤丹 种子层积过程中营养物质的代谢变化研究(DynamicchangesofnutrientsofPaeoniaostii FengDan seedduringitsdormancybreaking).南京林业大学学报(自然科学版),2021,45(1):70-78.孙佳彤,国艳娇,李㊀爽,周晨光,姜立泉,李㊀伟.基于CRISPR/Cas9的毛果杨bHLH106转录因子的功能研究(AfunctionalstudyofbHLH106transcriptionfactorbasedonCRISPR/Cas9inPopulustrichocarpa).南京林业大学学报(自然科学版),2021,45(6):15-23.田㊀力,徐骋炜,尚旭岚,洑香香.青钱柳药用优良单株评价与选择(EvaluationandselectiononsuperiorindividualsformedicinaluseofCyclo⁃caryapaliurus).南京林业大学学报(自然科学版),2021,45(1):21-28.田呈明,王笑连,余㊀璐,韩㊀珠.林木与病原菌分子互作机制研究进展(Areviewonthestudiesofmolecularinteractionbetweenforesttreesandphytopathogens).南京林业大学学报(自然科学版),2021,45(1):1-12.童㊀龙,李红艳,刘小明,李㊀彬,陈丽洁,陈桂兰,曾小英,耿养会.不同栽培基料对竹荪农艺性状和主要营养成分的影响(EffectsofdifferentcultivatedformulasontheagronomiccharacteristicsandnutritionalvalueofDictyophoraindusiata).南京林业大学学报(自然科学版),2021,45(3):30-36.万雅雯,傅华君,时培建,林树燕.变温对毛竹种子萌发及幼苗生长的影响(EffectsofvariabletemperaturesonseedgerminationandseedlinggrowthofPhyllostachysedulis).南京林业大学学报(自然科学版),2021,45(4):97-106.王㊀冰,张鹏杰,张秋良.不同林型兴安落叶松林土壤团聚体及其有机碳特征(Characteristicsofthesoilaggregateanditsorganiccarbonindif⁃ferentLarixgmeliniiforesttypes).南京林业大学学报(自然科学版),2021,45(3):15-24.王㊀瑞,王国兵,徐㊀瑾,徐㊀晓.凋落物与蚯蚓对杨树人工林土壤团聚体分布及其碳氮含量的影响(Effectsoflitterfallsandearthwormsondistributionofsoilaggregatesandcarbonandnitrogencontentinpoplarplantations).南京林业大学学报(自然科学版),2021,45(3):25-29.王㊀玄,崔㊀鹏,丁晶晶,常㊀青.江苏南部沿海越冬水鸟群落结构及多样性分析(CommunitystructureanddiversityofoverwinteringwaterfowlsinsouthcoastofJiangsuProvince).南京林业大学学报(自然科学版),2021,45(5):178-184.王㊀圳,高亚军,闫凡峰,王晓伟,李华清,姜㊀雷.海滨城市道路绿化树种综合评价体系构建(Constructionofacomprehensiveassessmentsystemforroadgreeningtreespeciesincoastalcities).南京林业大学学报(自然科学版),2021,45(2):187-196.王福根,卫星杓,赵国春,贾黎明.无患子细根形态及垂直分布特征对配方施肥措施的响应(ResponsesofmorphologyandverticaldistributionoffinerootsinSapindusmukorossitoformulafertilization).南京林业大学学报(自然科学版),2021,45(4):58-66.王国兵,徐㊀瑾,徐㊀晓,阮宏华,曹国华.蚯蚓与凋落物对杨树人工林土壤酶活性的影响(Effectsofearthwormsandlitterfallsonthesoilenzymeactivitiesofpoplarplantations).南京林业大学学报(自然科学版),2021,45(3):8-14.王君杰,姜立春.基于线性分位数组合的兴安落叶松冠幅预测(PredictingcrownwidthforLarixgmeliniibasedonlinearquantilesgroups).南京林业大学学报(自然科学版),2021,45(5):161-170.王立超,陈凤毛,仇才楼,唐进根,丁学农,任吉星.坡面方胸材小蠹鉴定与风险分析(IdentificationandriskanalysesofEuwallaceainterjectus).南京林业大学学报(自然科学版),2021,45(5):201-208.王路君,蔡春菊,唐晓鹿,范少辉.硬头黄竹地上生物量分配特征及模型构建(AbovegroundbiomassallocationpatternsandmodelconstructionofBambusarigida).南京林业大学学报(自然科学版),2021,45(1):189-196.王明哲,崔晓阳,李斯雯,张伟波,赵华晨.大兴安岭北端地形因子对针叶林土壤黑碳储量的影响(EffectsoftopographicfactorsonsoilblackcarbonstorageinconiferousforestsatthenorthendofGreaterKhinganMountains).南京林业大学学报(自然科学版),2021,45(1):151-158.王培龙,杨㊀妮,张傲然,唐努尔㊃塞力克,李㊀爽,高彩球.刚毛柽柳ThPCS1基因克隆与镉胁迫应答分析(CloningThPCS1geneofTamarixhispidatoimprovecadmiumtolerance).南京林业大学学报(自然科学版),2021,45(3):71-78.542. All Rights Reserved.南京林业大学学报(自然科学版)第45卷王润松,孙㊀源,徐涵湄,曹国华,沈彩芹,阮宏华.施用沼液对杨树人工林细根生物量的影响(Effectsofbiogasslurryapplicationonfinerootbiomassofpoplarplantations).南京林业大学学报(自然科学版),2021,45(4):123-129.王润松,徐涵湄,曹国华,沈彩芹,阮宏华.施用沼液对杨树人工林细根形态特征的影响(Effectsofapplyingbiogasslurryonthemorphologicalcharacteristicsoffinerootsofpoplarplantations).南京林业大学学报(自然科学版),2021,45(5):119-124.王树梅,王㊀波,范少辉,肖㊀箫,夏㊀雯,官凤英.带状采伐对毛竹林土壤细菌群落结构及多样性的影响(Influenceofstripcuttingmanage⁃mentonsoilbacterialcommunitystructureanddiversityinPhyllostachysedulisstands).南京林业大学学报(自然科学版),2021,45(2):60-68.王树梅,范少辉,肖㊀箫,郑亚雄,周㊀阳,官凤英.带状采伐对毛竹地上生物量分配及异速生长的影响(Effectsofstripcuttingonabovegroundbiomassaccumulationandallocation,andallometricgrowthofPhyllostachysedulis).南京林业大学学报(自然科学版),2021,45(5):19-24.王卫卫,张应良.退耕还林农户技术禀赋对经果林换种的影响(Theimpactoffarmers technicalendowmentontheadoptingnewvarietiesoffruitforest).南京林业大学学报(自然科学版),2021,45(5):215-222.王羽尘,马健霄,刘宇航,白莹佳.基于烟气扩散特征的林区隧道火灾人群疏散模型(Theevacuationmodelofforesttunnelfirebasedonthecharacteristicsofsmokediffusion).南京林业大学学报(自然科学版),2021,45(6):177-184.王志鹏,王㊀薇,邢思懿.城市公园绿地特征和使用方式与人群健康关系研究(Astudyontherelationshipbetweenthecharacteristicsandusageofurbanparkgreenspaceandpopulationhealth).南京林业大学学报(自然科学版),2021,45(5):223-231.王竹雯,国艳娇,李㊀爽,周晨光,姜立泉,李㊀伟.基于CRISPR/Cas9的毛果杨PtrHBI1基因功能解析(FunctionalanalysesofPtrHBI1geneinPopulustrichocarpabasedonCRISPR/Cas9).南京林业大学学报(自然科学版),2021,45(6):31-39.王子芝,李㊀玥,华世明,周俊宏,刘文斗,廖声熙.基于生态保护加权的普达措国家公园功能分区研究(FunctionalzoningofPotatsoNationalParkbyecologicalprotectionweighting).南京林业大学学报(自然科学版),2021,45(6):225-231.韦庆钰,黄海龙,吴纯泽,苏嘉熙,卫㊀星.3种倍性青杨扦插苗对覆膜滴肥的生长响应(ResponseofPopuluscathayanacuttingseedlingsofthreeploidytypestofertilizerunderfilmmulchinganddripirrigation).南京林业大学学报(自然科学版),2021,45(5):93-101.魏㊀宁,李国雷,蔡梦雪,史文辉,刘㊀文,薛㊀柳,李进宇.缓释肥施氮量对4种国外栎苗木质量及移栽成活率的影响(Effectsofslow⁃releasefertilizationratesonseedlingqualityandfieldsurvivalratesoffourexoticoaks).南京林业大学学报(自然科学版),2021,45(3):53-60.魏龙鑫,章异平,李艺杰,张玉茹.栓皮栎叶片和枝条非结构性碳水化合物调配关系研究(Allocationofnon⁃structuralcarbohydrates(NSC)con⁃tentsinleavesandbranchesofQuercusvariabilisduringitsgrowthprocess).南京林业大学学报(自然科学版),2021,45(2):96-102.吴㊀红,燕丽萍,李成忠,夏㊀群,周㊀霞,赵宝元.槭树属常见树种翅果性状多样性与风传播特征分析(MorphologicalcharacteristicsandwinddispersalcharacteristicsofsamaraofcommonAcerspecies).南京林业大学学报(自然科学版),2021,45(2):103-110.吴丽君,游云飞,陈㊀达,陈文荣,李文芳. 黄樽 薄叶金花茶组培苗生根与移栽技术研究(Optimizationoftherootingandtransplantationte⁃chinquesoftissue⁃culturedshootsofCamelliachrysanthoides Huangzun ).南京林业大学学报(自然科学版),2021,45(3):117-122.吴其超,马㊀燕,李呈呈,许建军,么燕君,臧德奎.桂花新品种 冬荣 (Osmanthusfragrans Dongrong :anewcultivarofosmanthus).南京林业大学学报(自然科学版),2021,45(3):245-246.吴文清,许克福.合肥环城公园开放空间与游憩行为关系研究(RecreationalpreferencesamongdifferentopenspacesinaringcityparkofHefei).南京林业大学学报(自然科学版),2021,45(6):217-224.夏雯雯,李㊀想,王钰祺,徐㊀驰,刘茂松.互花米草与盐地碱蓬群落交错带土壤因子的梯度变化特征(DistributionofsoilfactorsacrossthehabitatgradientofSpartinaalternifloraandSuaedasalsacommunities).南京林业大学学报(自然科学版),2021,45(3):37-44.辛士冬,何㊀培,姜立春.不同矫正位置对落叶松分位数削度方程预测精度的影响(EffectsofdifferentcalibrationpositionsonpredictionprecisionofquantiletaperfunctionforLarixgmelinii).南京林业大学学报(自然科学版),2021,45(1):182-188.熊㊀瑶,严㊀妍.基于人体热舒适度的江南历史街区空间格局研究 以南京高淳老街为例(EffectsofspatialdesignandmicroclimateonhumanthermalcomfortintheregionsouthoftheYangtzeRiver:acasestudyofoldstreetinGaochun,Nanjing).南京林业大学学报(自然科学版),2021,45(1):219-226.熊光康,厉月桥,熊有强,段爱国,曹德春,孙建军,聂林芽,盛炜彤.低密度造林对杉木生长㊁形质和材种结构的影响(Effectsoflowstanddensityafforestationonthegrowth,stem⁃formandtimberassortmentstructureofCunninghamialanceolataplantations).南京林业大学学报(自然科学版),2021,45(3):165-173.徐晶园,圣倩倩,王伟希,刘聪哲,祝遵凌.南京典型城市道路植物多样性与土壤因子的耦合关系(Modelingthedegreeofcouplingandinterac⁃tionbetweenplantcommunitydiversityandsoilpropertiesontypicalurbanroadsinNanjing).南京林业大学学报(自然科学版),2021,45(6):119-126.徐圆圆,周思维,陈㊀仲,赵国春,刘济铭,王立宪,王㊀昕,贾黎明,张端光.无患子不同器官中的总皂苷和总黄酮含量(ContentsofthetotalsaponinsandtotalflavonoidsindifferentorgansofSapindusmukorossi).南京林业大学学报(自然科学版),2021,45(4):83-89.许嘉麟,谈家金,郝德君.蜡样芽孢杆菌NJSZ-13菌株对松材线虫产卵和繁殖的影响(EffectofBacilluscereusNJSZ⁃13strainonovipositionandreproductionofBursaphelenchusxylophilus).南京林业大学学报(自然科学版),2021,45(5):209-214.许中秋,隋德宗,谢寅峰,王俊毅.两个乌桕新品种苗木光合特性比较(ComparisonofphotosyntheticcharacteristicsoftwonewTriadicasebiferavarieties).南京林业大学学报(自然科学版),2021,45(1):93-100.薛蓓蓓,田国双.基于碳汇木材复合经营目标的综合效益及影响因素分析(Analysisofcomprehensivebenefitsandinfluencingfactorsbasedonthecombinedeconomicvalueofcarbonsequestrationandtimberbenefits).南京林业大学学报(自然科学版),2021,45(2):205-212.642. All Rights Reserved.㊀第6期‘南京林业大学学报(自然科学版)“2021年论文题录(作者)索引闫想想,王秋华,缪秀丽,韩永涛,龙腾腾.昆明西山林场5种可燃物的火行为研究(FirebehavioroffivekindsoffuelsinXishanForestFarm,KunmingCity).南京林业大学学报(自然科学版),2021,45(1):197-204.杨㊀南,崔允姬,王㊀茜,王曙光.木竹的花器官形态与解剖结构研究(AstudyonthemorphologyandanatomicalstructureofBambusarutilaspiklets).南京林业大学学报(自然科学版),2021,45(4):90-96.杨㊀意,刘㊀波,叶建仁,苏禄晖.水杉赤枯病综合营林生态控制技术研究(AstudyontheecologicalcontrolofredblightofMetasequoiaglyp⁃tostroboidesbyintegratedforestmanagement).南京林业大学学报(自然科学版),2021,45(6):90-98.杨红强,余智涵.全球木质林产品碳科学研究动态及未来的重点问题(Researchtrendsandfuturekeyissuesofglobalharvestedwoodproductscarbonscience).南京林业大学学报(自然科学版),2021,45(4):219-228.杨清平,陈双林,郭子武,郑㊀进.摘花和打顶措施对毛竹林下多花黄精块茎生物量积累特征的影响(ResponsesoftuberbiomassaccumulationanditsallometrytotoppingandflowerpluckingmeasuresofPolygonatumcyrtonemagrownunderPhyllostachysedulisforests).南京林业大学学报(自然科学版),2021,45(2):165-170.杨瑞珍,张焕朝,胡立煌,范之馨.接种AMF及施氮对滨海盐土氮矿化的影响(EffectsofAMFinoculationandnitrogenapplicationonnitrogenmineralizationofcoastalsalinesoil).南京林业大学学报(自然科学版),2021,45(2):145-152.姚晶晶,冯象千,肖㊀贺,郑㊀豫,张成梁.不同固废及其处理产物对黄骅港盐碱土的改良效果(Improvementeffectsofdifferentsolidwasteandtheirdisposalbyproductsonsaline⁃alkalisoilinHuanghuaPort).南京林业大学学报(自然科学版),2021,45(3):45-52.姚正明,田旭琴,蒙慧理,邓云飞.爵床科凹苞马蓝在贵州的分布新记录及其补充描述(ThenewdistributionofStrobilanthesretusa(Acanthaceae)inGuizhouwithsupplementarydescription).南京林业大学学报(自然科学版),2021,45(4):177-182.伊贤贵,董㊀鹏,谢春平,彭智奇,杨国栋,董京京,钟育谦,翟飞飞,王贤荣.江苏宜兴龙池山自然保护区固定样地物种组成分析(AnanalysisonspeciescompositionofapermanentplotontheLongchiMountain,YixingCity,JiangsuProvince).南京林业大学学报(自然科学版),2021,45(6):159-168.尹艳楠,谈家金,李梦伟,许嘉麟,郝德君.蜡样芽孢杆菌NJSZ-13菌株防治松材线虫病研究(AstudyonthebiocontrolofpinewiltdiseasebyBacilluscereusNJSZ⁃13).南京林业大学学报(自然科学版),2021,45(3):152-158.于松平,刘泽彬,郭建斌,王彦辉,于澎涛,王㊀蕾.六盘山华北落叶松林分蒸腾特征及其影响因素(StandtranspirationcharacteristicsofLarixprincipis⁃rupprechtiiplantationandtheirinfluencingfactorsinLiupanMountain).南京林业大学学报(自然科学版),2021,45(1):131-140.原雅楠,李正才,王㊀斌,张雨洁,黄盛怡.不同林龄榧树根㊁枝㊁叶的C㊁N㊁P化学计量及内稳性特征(Ecologicalstoichiometryinleaves,branchesandrootsofTorreyagrandiswithdifferentforestagesanditsstoichiometrichomoeostasis).南京林业大学学报(自然科学版),2021,45(6):135-142.袁金玲,马婧瑕,钟远标,岳晋军.基于SSR标记的丛生竹杂种鉴定㊁遗传分析和指纹图谱构建(SSR⁃basedhybrididentification,geneticanalysesandfingerprintdevelopmentofhybridizationprogeniesfromsympodialbamboo(Bambusoideae,Poaceae)).南京林业大学学报(自然科学版),2021,45(5):10-18.袁婷婷,路远峰,谢寅峰,马迎莉,吴㊀桐,倪㊀震.硼钼铜微肥配施对太子参光合特性的影响(Effectsofcombinedapplicationofboron⁃molybdenum⁃coppermicrofertilizersonphotosyntheticcharacteristicsofPseudostellariaheterophylla).南京林业大学学报(自然科学版),2021,45(4):130-136.苑兆和,陈立德,张心慧,赵玉洁.果树分子育种研究进展(Advancesinmolecularbreedingoffruittrees).南京林业大学学报(自然科学版),2021,45(4):1-12.岳晋军,朱燕琳,袁金玲.绿竹笋用林长周期母竹留养模式研究(StudyonmanagementmodeloflongperiodstockingbambooconservationinDendrocalamopsisoldhami).南京林业大学学报(自然科学版),2021,45(5):31-37.臧明月,李㊀璇,方炎明.基于SSR标记的白栎天然居群遗传多样性分析(GeneticdiversityanalysisamongnaturalpopulationsofQuercusfabribasedonSSRmarkers).南京林业大学学报(自然科学版),2021,45(1):63-69.张㊀恒,崔孟然,单延龙,王㊀飞.中蒙边境典型草原草本可燃物燃烧性研究(StudyonflammabilityofherbaceousfuelintypicalgrasslandofChina⁃Mongoliaborder).南京林业大学学报(自然科学版),2021,45(5):171-177.张㊀磊,童㊀龙,谢锦忠,李俞佳,张㊀玮.不同灌水时间下毛竹伐桩根系化学计量及生理特性变化(Changesinchemicalmetrologyandphysio⁃logicalcharacteristicsofPhyllostachysedulisstumprootsunderdifferentirrigationtimes).南京林业大学学报(自然科学版),2021,45(5):25-30.张㊀琳,程亚男,张㊀欣,杨伟婷,孔庆涛,谢东锋.两种植物生长调节剂对木槿插穗生根的影响(EffectsoftwoplantgrowthregulatorsonrootingofHibiscussyriacuscuttings).南京林业大学学报(自然科学版),2021,45(3):123-129.张㊀濛,续高山,滕志远,刘关君,张秀丽.模拟酸雨对小黑杨幼苗生长和光合特性的影响(Effectsofsimulatedacidrainongrowthandphotosyn⁃theticphysiologicalcharacteristicsofPopulussimoniiˑP.nigra).南京林业大学学报(自然科学版),2021,45(6):57-64.张㊀琪,钱㊀滕,王㊀欢,朱铭玮,李淑娴.加拿大紫荆种子硬实性解除及其吸水特性研究(HardnessbreakingandmechanismsofwaterabsorptioninCerciscanadensisseeds).南京林业大学学报(自然科学版),2021,45(3):137-142.张㊀群,及晓宇,贺子航,王智博,田增智,王㊀超.白桦BpGRAS1基因的克隆及耐盐功能分析(CloningandsalttoleranceanalysisofBpGRAS1geneinBetulaplatyphylla).南京林业大学学报(自然科学版),2021,45(5):38-46.张㊀馨,马苗苗,吕婉秋,LEEJoobin,杨静莉.大青杨PuZFP103基因的序列特征及逆境胁迫的表达分析(Sequencecharacteristicsandexpres⁃sionpatternanalysesofPuZFP103geneunderabioticstressinPopulusussuriensis).南京林业大学学报(自然科学版),2021,45(1):36-44.742. 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biomassBiomass: An Essential Source for Sustainable DevelopmentIntroduction:Biomass refers to organic materials derived from living, or recently living, organisms. It is a valuable and renewable source of energy that holds great potential for sustainable development. Biomass can be utilized in various forms, such as wood, crop residues, animal manure, and dedicated energy crops. This article aims to explore the importance of biomass as an alternative energy source, its potential applications, and the challenges associated with its implementation.The Significance of Biomass:1. Renewable Energy Source:One of the most significant advantages of biomass is that it is renewable. Unlike fossil fuels, which take millions of years to form, biomass can be continually produced through sustainable agricultural practices. This characteristic makesbiomass a key component in the quest for reducing carbon emissions and combating climate change.2. Energy Diversification:Biomass offers diversification in energy sources, reducing dependence on fossil fuels. By using biomass for electricity generation, heating, and transportation fuels, countries can improve energy security and reduce their reliance on imported energy. This diversification also helps mitigate the price volatility often associated with fossil fuels.3. Waste Management:Biomass utilization plays a crucial role in waste management. By converting organic waste into energy, biomass systems can reduce the burden on landfills and prevent the release of harmful greenhouse gases during decomposition. This not only minimizes environmental pollution but also provides a sustainable solution for waste disposal.Applications of Biomass:1. Heat and Power Generation:Biomass can be used to produce heat and electricity through various technologies, such as combustion, gasification, and anaerobic digestion. Biomass power plants generate electricity by burning biomass to produce steam, which drives a turbine connected to a generator. Heat generated during this process can be utilized for district heating systems or industrial processes, making biomass a versatile energy source.2. Biofuels:Biomass can be converted into liquid biofuels, such as ethanol and biodiesel, which are used as alternatives to fossil fuels. Ethanol, produced by fermenting sugars present in biomass, is commonly blended with gasoline to reduce carbon emissions. Biodiesel, derived from vegetable oils or animal fats, can be directly used in diesel engines or blended with petroleum diesel. Biofuels offer a cleaner and more sustainable option for transportation, reducing greenhouse gas emissions and enhancing energy security.3. Biorefineries:Biomass can be processed in biorefineries to obtain a range of value-added products, such as chemicals, materials, and bio-based products. Biorefineries integrate various conversion technologies to extract maximum value from different biomass feedstocks. This approach utilizes a holistic approach to biomass utilization, creating a sustainable and efficient industry.Challenges and Opportunities:1. Resource Availability:The sustainable utilization of biomass requires careful consideration of resource availability. It is crucial to ensure that biomass is sourced responsibly, without causing deforestation or compromising food security. Sustainable biomass management practices, such as planting dedicated energy crops and utilizing agricultural residues, can help overcome these challenges.2. Technological Advancements:To maximize the potential of biomass, ongoing research and development are essential. Technological advancements are needed to improve biomass conversion processes, enhance efficiency, and reduce costs. Innovation in areas such as pretreatment, fermentation, and thermochemical conversion can significantly improve the viability of biomass as an energy source.3. Policy Support:Governments play a vital role in promoting the adoption of biomass as an energy source. Policy support, such as feed-in tariffs, tax incentives, and research funding, can encourage investment in biomass projects and create a favorable market environment. Clear and supportive policies also help overcome barriers and promote the widespread use of biomass.Conclusion:Biomass is a valuable and renewable resource with immense potential for sustainable development. Its utilization offers numerous advantages, including being a renewable energy source, waste management solution, and diversifying the energy mix. Biomass can be applied in various forms, rangingfrom heat and power generation to biofuels and biorefineries. While challenges related to resource availability and technological advancements exist, with supportive policies and continued research, biomass can contribute significantly to a cleaner and more sustainable future.。
小五台山天然青杨林雌雄群体的生长差异李霄峰;王志峰;黄尤优;王碧霞;陈坚【摘要】通过对小五台山天然青杨种群的野外调查,并使用胸径与株高的异速生长模型来分析其雌雄群体间的生长差异,以探究雌雄异株植物青杨在性成熟条件和形态特征中是否存在性间差异.结果表明:(1)在青杨生长过程中,胸径随年龄呈指数型增长,而株高随年龄呈对数型增长;(2)雌雄植株的性成熟条件不同.雌株进入性成熟阶段的最低年龄和胸径都小于雄株;(3)青杨高径生长过程存在性别差异.雌株的异速生长指数显著大于雄株(P=0.024).表明天然青杨种群中雌株一般性成熟较早,成熟后营养生长偏重于胸径增粗;而雄株性成熟较晚,营养生长偏重于植株增高.相对于雄株,雌株具有较高的树干机械强度.【期刊名称】《广西植物》【年(卷),期】2013(033)003【总页数】5页(P416-420)【关键词】雌雄异株;青杨;生长特征;雌雄差异;性成熟;异速生长【作者】李霄峰;王志峰;黄尤优;王碧霞;陈坚【作者单位】西华师范大学,西南野生动植物资源保护教育部重点实验室,四川,南充,637009;张家口市农业环境与农产品质量管理站,河北,张家口,075000;西华师范大学,西南野生动植物资源保护教育部重点实验室,四川,南充,637009;西华师范大学,西南野生动植物资源保护教育部重点实验室,四川,南充,637009;西华师范大学,西南野生动植物资源保护教育部重点实验室,四川,南充,637009;四川省旺苍县米仓山自然保护区管理局,四川,广元,628200【正文语种】中文【中图分类】Q948.11雌雄异株植物由于繁殖功能在个体完全分离,因而生殖投入的差异会导致不同性别的个体在生长速率和适生环境方面表现出不同特征。
研究认为由于雌株的繁殖成本较高,因而性成熟后其营养生长速率会逐渐减缓,最终雄株的个体大小将超过雌株(Obeso et al.,1998;Wallace &Rundel,1979;Gross &Soule,1981)。
ReviewThe importance of almond (Prunus amygdalus L.)and its by-productsAli Jahanban Esfahlan a,*,Rashid Jamei a ,Rana Jahanban Esfahlan ba Department of Biology,Faculty of Science,Urmia University,Urmia,IranbDepartment of Medical Biotechnology,Faculty of Medicine,Tabriz University of Medical Science,Tabriz,Irana r t i c l e i n f o Article history:Received 29June 2009Received in revised form 2September 2009Accepted 16September 2009Keywords:Almond (Prunus amygdalus L .)By-products Importancea b s t r a c tAlmond fruit consists of three or correctly four portions:kernel or meat,middle shell,outer green shell cover or almond hull and a thin leathery layer known as brown skin of meat or seedcoat.The nutritional importance of almond fruit is related to its kernel.Other parts of fruit such as shells and hulls were used as livestock feed and burned as fuel.In the past decades,different phenolic compounds were characterised and identified in almond seed extract and its skin,shell and hull as almond by-products.In addition,poly-phenols are abundant micronutrients in the human diet,and evidence for their role in the prevention of degenerative diseases such as cancer and cardiovascular diseases is emerging.The health effects of poly-phenols depend on the amount consumed and on their bioavailability.In this contribution,various pheno-lic compounds present in almond and its by-products,their antioxidant properties and potential use as natural dietary antioxidant,as well as their other beneficial compounds and applications are reviewed.Ó2009Elsevier Ltd.All rights reserved.Contents 1.Introduction .........................................................................................................3492.Almond (P.amygdalus L .)fruit characteristics ..............................................................................3503.Different parts of almond (P.amygdalus L .)fruit ............................................................................3503.1.Almond meat...................................................................................................3503.2.Almond meat brown skin.........................................................................................3513.3.Almond green shell cover (hull)....................................................................................3523.4.Almond shell ...................................................................................................3544.Potential application of almond by-products ...............................................................................3544.1.Almond shell as heavy metal adsorbent .............................................................................3544.2.Almond shell as dyes adsorbent....................................................................................3554.3.Almond shell as growing media....................................................................................3554.4.Almond shell as a rich source in preparing activated carbons............................................................3564.5.Almond shell as a source for the production of xylo-oligosaccharides (XOs)................................................3564.6.Almond by-products as dietary antioxidants..........................................................................3575.Antioxidant and antiradical activity of almond and its by-products ............................................................357References ..........................................................................................................3581.IntroductionNuts are known as a source of nutritious food with high lipid content.Replacing half of the daily fat intake with nuts has been known to lower total and LDL cholesterol levels significantly in hu-mans (Abbey,Noakes,Belling,&Nestel,1994).The observed blood cholesterol lowering effects of nuts were far better than what was predicted according to their dietary fatty acid profiles (Kendall,Jenkins,Marchie,Parker,&Connelly,2002;Kris-Etherton et al.,1999).Research also shows a connection between regular nut con-sumption and decreased incidence of coronary heart disease (Dreher,Maher,&Kearney,1996).These beneficial physiological effects suggest that bioactive compounds of nuts may possess lipid altering activities due to additive/synergistic effects and/or0308-8146/$-see front matter Ó2009Elsevier Ltd.All rights reserved.doi:10.1016/j.foodchem.2009.09.063*Corresponding author.Tel.:+989355972580;fax:+984412776707.E-mail address:a.jahanban@ (A.J.Esfahlan).Food Chemistry 120(2010)349–360Contents lists available at ScienceDirectFood Chemistryj o u r n a l h o m e p a g e :/locate/foodcheminteractions with each other.Dietary antioxidants provide protec-tion against oxidative attack by decreasing oxygen concentration, intercepting singlet oxygen,preventingfirst-chain initiation by scavenging initial radicals,binding of metal ion catalysts,decom-posing primary products of oxidation to non-radical compounds, and chain breaking to prevent continuous hydrogen removal from substrates(Shahidi,1997;Wijeratne,Abou-zaid,&Shahidi,2006).Almonds(Prunus amygdalus),which belong to the Rosaceae family that also includes apples,pears,prunes,and raspberries, are one of the most popular tree nuts on a worldwide basis and rank number one in tree nut production.They are typically used as snack foods and as ingredients in a variety of processed foods, especially in bakery and confectionery products(Sang,Chen, et al.,2002).The United States is the largest almond producer in the world and most of the US almonds are grown in California (Jahanban,Mahmoodzadeh,Hasanzadeh,Heidari,&Jamei,2009; Wijeratne et al.,2006)in an area that stretches over400miles from Bakersfield to Red Bluff(Sathe et al.,2002).Over7000individual growers cultivate more than400,000acres of almonds.Almonds are California’s largest tree crop based on dollar value,acreage, and world distribution.Five major varieties of almonds grown in California include Nonpareil,Mission,California,Neplus Ultra, and Peerless.Of thefive groups listed,most almond production (about90%)falls into three major marketing categories of Nonpareil,California,and Mission Almonds are the seeds of varieties of P.amygdalus(Sang,Lapsley,et al.,2002).Almonds,when incorporated in the diet,have been reported to reduce colon cancer risk in rats(Davis&Iwahashi,2001)and in-crease HDL cholesterol and reduce LDL cholesterol levels in hu-mans(Hyson,Schneeman,&Davis,2002).Extracts of whole almond seed,brown skin,shell,and green shell cover(hull)possess potent free radical-scavenging capacities(Amarowicz,Troszynska, &Shahidi,2005;Jahanban et al.,2009;Moure,Pazos,Medina, Dominguez,&Parajo,2007;Pinelo,Rubilar,Sineiro,&Nunez, 2004;Siriwardhana,Amarowicz,&Shahidi,2006;Siriwardhana& Shahidi,2002;Wijeratne et al.,2006).These activities may be re-lated to the presence offlavonoids and other phenolic compounds in nuts.Almond hulls have been shown to serve as a rich source of three triterpenoids(about1%of the hulls),betulinic,urosolic,and oleanolic acids(Takeoka et al.,2000),as well asflavonol glycosides and phenolic acids(Sang,Lapsley,Rosen,&Ho,2002).In addition, Sang,Chen,et al.(2002),Sang,Lapsley,et al.(2002),and Sang, Lapsley,Rosen,et al.(2002)isolated catechin,protocatechuic acid, vanillic acid,p-hydroxybenzoic acid,and naringenin glucoside,as well as galactoside,glucoside,and rhamnoglucoside of3b-O-meth-ylquercetin and rhamnoglucoside of kaempferol.The production of almond hulls,which are mainly used in livestock feed,is estimated to exceed6million tons annually,thus being a potentially good source from which to extract antioxidants that are present,if any,in high quantities(Siriwardhana et al.,2006;Shahidi,Zhong, Wijeratne,&Ho,2009).2.Almond(P.amygdalus L.)fruit characteristicsThe peach-like edible almonds fruit(P.amygdalus)have three distinct parts:the inner kernel or meat,the middle shell portion, and an outer green shell cover or hull.Almond varieties vary in shell texture;therefore they are termed hard or soft shelled.The harvesting procedure starts when the almonds are partly dried on the trees(King,Miller,&Eldridge,1970).In addition the sweet almond is a stone fruit which have several unique features. It is commercially cultivated where there are long,hot,and Mediterranean like summers,such as those in Spain,Morocco, Armenia,Iran,Italy,California(USA),and Australia.It is unique, in that unlike others in its botanical family,such as peach,apricot and plum,where theflesh(mesocarp)of the fruit is eaten and the seed within its shell,or stone(endocarp)is discarded,the reverse is true for the almond early in its maturation cycle,for a period of a few weeks,the entire fruit(seed,endocarp and mesocarp)can be, and is,eaten,in several parts of the world.As the maturation cycle continues,the hull splits open.When dry,it may be readily sepa-rated from the shell.The almond pit,containing a kernel or edible seed,is the nut of commerce,the endocarp(shell),and mesocarp are separated for low value uses,such as cat litter and animal feed (Rabinowitz,1991,2002,2004).Shelled almonds may be sold as whole natural almonds or processed into various almond forms. The whole natural almonds have their shells removed but still re-tain their brown skins;blanched whole almonds have both their shells and skins ually,the removed skins are discarded (Sang,Chen,et al.,2002;Sang,Lapsley,et al.,2002;Sang,Lapsley, Rosen,et al.,2002).3.Different parts of almond(P.amygdalus L.)fruit3.1.Almond meatEdible nuts are cultivated under a variety of growing conditions and climates;they are globally popular and valued for their sen-sory,nutritional,and health attributes(Venkatachalam&Shridhar, 2006).Almond,scientifically known as Prunus dulcis,belongs to the family Rosaceae,almond tree is the number one tree nut produced on a global basis(Chen,Milbury,Lapsley,&Blumberg,2005; Wijeratne et al.,2006).It is especially spread through and well adapted to the whole Mediterranean region,from which about 28%of the world production is obtained.In fact,almond tree is an important crop,due to its fruits of high commercial value (Cordeiro&Monteiro,2001;Martins,Tenreiro,&Oliveira,2003; Moure et al.,2007).There is a great diversity of almonds which ex-hibit different productivity and yields of seed in the fruit(Martinez, Granado,Montane,Salvado,&Farriol,1995).Almond,with or without the brown skin,is consumed as the whole nut or used in various confectioneries(Wijeratne et al.,2006).It is well known that fruits and nuts contain a wide variety of phenolic acids and flavonoids that are predominantly conjugated with sugars or other polyols via O-glycosidic bonds or ester bonds(Milbury,Chen, Dolnikowski,&Blumberg,2006)and its consumption has been associated with reduced risk of chronic diseases(Pellegrini et al., 2006).The Prunus genus is reported to have interesting biological properties such as sedative,anti-inflammatory,anti-hyperlipi-demic,anti-tumoural and antioxidant activities(Donovan,Meyer, &Waterhouse,1998;Sang,Chen,et al.,2002;Wang,Nair, Strasburg,Booren,&Gray,1999).Apart from its nutritional value, almond is reported to have beneficial effects on blood cholesterol level and lipoprotein profile in humans.In addition,almonds,when used as snacks and in diets of hyperlipidemic subjects,significantly reduced coronary heart disease factors.A longterm supplementa-tion of almond showed spontaneous nutrient modification of an individual’s habitual diet that closely matched the recommen-dations to prevent cardiovascular and other chronic diseases (Wijeratne et al.,2006).Few studies reporting almond seed antioxidant potential are available.Research has only been made on bioactive compounds and their antioxidant activity in almond hulls(Takeoka et al., 2000;Sang,Chen,et al.,2002;Sang,Lapsley,et al.,2002;Takeoka &Dao,2003;Rabinowitz,2004;Jahanban et al.,2009),almond skins(Sang,Chen,et al.,2002)and almond shells(Jahanban et al.,2009;Pinelo et al.,2004).Even though it has already been demonstrated that individual almond components(Takeoka et al.,2000;Sang,Chen,et al.,2002;Sang,Lapsley,et al.,2002; Takeoka&Dao,2003;Pinelo et al.,2004;Rabinowitz,2004;350 A.J.Esfahlan et al./Food Chemistry120(2010)349–360Jahanban et al.,2009)have antioxidant potential,scientific infor-mation on antioxidant properties of whole almond is still rather scarce.Hence,the evaluation of such properties remains an inter-esting and valuable task,particularly forfinding new sources for natural antioxidants,functional foods and nutraceuticals.Barreira,Ferreira,Oliveira,and Pereira(2008)studied the anti-oxidant properties of different almond kernels(maintaining the brown skin)cultivars(cv.),either regional(Casanova,Duro Italiano,Molar,Orelha de Mula and Pegarinhos cv.)or commercial (Ferraduel,Ferranhês,Ferrastar and Guara cv.)through several chemical and biochemical assays such as DPPH radical-scavenging activity,reducing power,inhibition of b-carotene bleaching,inhibi-tion of oxidative haemolysis in erythrocytes,induced by2,20-azo-bis(2-amidinopropane)dihydrochloride(AAPH),and inhibition of thiobarbituric acid reactive substances(TBARS)formation in brain cells.Bioactive compounds such as phenols andflavonoids have been obtained and correlated to antioxidant activity.The results obtained were quite heterogeneous,revealing significant differ-ences amongst the cultivars assayed.Duro Italiano cv.revealed better antioxidant properties,presenting lower EC50values in all assays,and the highest antioxidants contents.The protective effect of this cultivar on erythrocyte biomembrane haemolysis was main-tained over a4h period.Additionally,different almond cultivars revealed significant variation in antioxidant activity,correlating with the amounts of bioactive compounds present.Using an HPLC method,phenolic compounds such as vanillic, caffeic,p-coumaric,ferulic acids(after basic hydrolysis),quercetin, kaempferol,isorhamnetin(after acidic hydrolysis),delphinidin and cyanidin(after n-butanol/HCl hydrolysis)as well as procyanidins B2and B3were determined in almond seed extract(Tables1–3, 6and8).Dominant compounds were procyanidins B2and B3 (Amarowicz et al.,2005).3.2.Almond meat brown skinTheflesh of almond seed is encased in a brown leathery coat-ing,called the seedcoat,which protects the almond from oxida-tion and microbial contamination.Many food applications of almonds in bakery and confectionary items,cereals,snack formu-lations,and marzipan,require theflesh of the almond alone without the seedcoat(Frison-Norrie&Sporns,2002).Almond (P.amygdalus)skins are agricultural by-products that are a source of phenolic compounds and are produced upon processing of al-monds in large amounts.Almond skins,resulting from hot water blanching process,are ground and used as animal feed or burned as fuel in processing plants(Harrison&Were,2007).The skins constitute about4%of the almond fruit,and are a readily avail-able source of phenolics(Chen et al.,2005).These phenolic com-pounds inhibit lipid oxidation by scavenging free radicals, chelating metals,activating antioxidant enzymes,reducing tocopherol radicals and inhibiting enzymes that cause oxidation reactions(Heim,Tagliaferro,&Bobilya,2002).Yet,recent investi-gations into the phytochemical composition of almond skins have shown that the seedcoats may contain many potentially benefi-cial compounds,opening up new possibilities for adding value to almond seedcoats.Takeoka et al.(2000)described three trit-erpenoids,betulinic acid,oleanoic acid,and ursolic acid,which have been reported to possess anti-inflammatory(Singh,Singh, &Bani,1994),anti-HIV(Kashiwada et al.,1998),and anti-cancer activities(Pisha et al.,1995).Various phenolic compounds have been identified in almond seed coats.Four differentflavonol gly-cosides,namely isorhamnetin rutinoside,isorhamnetin glucoside, kaempferol rutinoside and kaempferol glucoside have been re-ported in almond seedcoats(Frison&Sporns,2002;Frison-Norrie &Sporns,2002;Wijeratne et al.,2006)(Table4).Other investiga-tors have likewise identified phenolic compounds in almond skins including quercetin glycosylated to glucose,galactose and rham-nose,kaempferol,naringenin,catechin,protocatechuic acid,vanil-lic acid and a benzoic acid derivative(Chen et al.,2005;Monagas, Garrido,Lebron-Aguilar,Bartolome,&Gomez-Cordoves,2007) (Tables1and3–5).In addition,the isolation and identification of phenolic com-pounds in almond skins has been reported by Sang,Chen,et al. (2002).In this study,nine phenolic compounds were isolated from the ethyl acetate and n-butanol fractions of almond(P.amygdalus) skins.On the basis of NMR data,MS data,and comparison with the literature,these compounds were identified as3b-O-methylqu-ercetin3-O-a-D-glucopyranoside(1);3b-O-methylquercetin3-O-a-D-galactopyranoside(2);3b-O-methylquercetin3-O-R-L-rhamnopyranosyl-(1?6)-a-D-glucopyranoside(3);kaempferol 3-O-R-L-rhamnopyranosyl-(1?6)-a-D-glucopyranoside(4); naringenin7-O-a-D-glucopyranoside(5);catechin(6);proto-catechuic acid(7);vanillic acid(8);and p-hydroxybenzoic acid (9)(Tables1and3–5).All of these compounds have been isolated from almond skins for thefirst time.2,2-Diphenyl-1-pic-rylhydrazyl(DPPH)radical-scavenging activities of compounds 1–9showed that compounds6and7possessed very strong DPPH radical-scavenging pounds1–3,5,8,and9showed strong activity,whereas compound4exhibited a very weak activity.In another investigation,an exhaustive study of the phenolic composition of almond(Prunus dulcis(Mill.) D.A.Webb)skinsTable1Content of hydroxybenzoic acids and aldehydes in different parts of almond.Hydroxybenzoic acids and aldehydes Content ReferencesKernel Skin Shell Hullp-Hydroxybenzoic acid– 6.88–5.33a––Monagas et al.(2007)–+––Sang,Chen,et al.(2002),Sang,Lapsley,et al.(2002),and Sang,Lapsley,Rosen,et al.(2002) Vanillic acid0.10b–––Amarowicz et al.(2005)–+––Sang,Chen,et al.(2002),Sang,Lapsley,et al.(2002),and Sang,Lapsley,Rosen,et al.(2002)–14.5–7.65a––Monagas et al.(2007) Protocatechuic acid–32.0–14.5a––Monagas et al.(2007)++–+Wijeratne et al.(2006)–+––Sang,Chen,et al.(2002),Sang,Lapsley,et al.(2002),and Sang,Lapsley,Rosen,et al.(2002) Protocatechuic aldehyde–20.1–11.6a––Monagas et al.(2007)+Present.–Not found.a l g/g.b mg catechin equivalents/g80%aqueous acetone extract.A.J.Esfahlan et al./Food Chemistry120(2010)349–360351carried out in order to evaluate their potential application as a functional food ingredient(Monagas et al.,2007).Using the HPLC–DAD/ESI–MS technique,a total of33compounds corre-sponding toflavanols,flavonols,dihydroflavonols andflavanones, and other non-flavonoid compounds identified.Peaks correspond-ing to another23structure-related compounds were also detected. MALDI–TOF MS was employed to characterise almond skin pro-anthocyanidins,revealing the existence of a series of A-and B-type procyanidins and propelargonidins up to heptamers,and A-and B-type prodelphinidins up to hexamers(Table3).Results showed thatflavanols andflavonol glycosides were the most abundant phenolic compounds in almond skins,representing up to38–57% and14–35%of the total quantified phenolics,respectively (Tables4–6).Due to their antioxidant properties,measured as oxy-gen-radical absorbance capacity(ORAC)at0.398–0.500mmol Trol-ox/g,almond skins can be considered as a value-added by-product for potential use as dietary antioxidant ingredients.3.3.Almond green shell cover(hull)The mesocarp of almond becomes dry,leathery,and astringent to the taste,reflecting the fact that the mature almond mesocarp has unusually a high concentration offlavonoids compared to its botanical relatives,as well as to other fruits.This is thought to be a consequence of the length of time that the mesocarp is subjected to intense heat,ultraviolet radiation,and pest infestation,as the flavonoids play protective roles against all of these stress factors. The extended maturation period of the mesocarp,flowing into remarkably stable senescence period,also allows for biosynthesis of lignans in the mesocarp,compared to the near absence of these compounds in other fruits.The mesocarp in senescence,following harvest of the nut meats,remains remarkably stable in that it re-tains its high sugars,flavonoids,and lignan content,for years,so long as the mesocarps,referred to as hulls,remain in their dry har-vested condition,having approximately8–20%moisture content,Table2Content of hydroxycinnamic acids in different parts of almond.Hydroxycinnamic acids Content ReferencesKernel Skin Shell HullFerulic acid0.02b–––Amarowicz et al.(2005)Trace 2.19±0.01c– 2.71±0.02c Siriwardhana et al.(2006) Sinapic acid Trace9.51±0.03c–9.92±0.02c Siriwardhana et al.(2006) Caffeic acid0.01b–––Amarowicz et al.(2005)Trace Trace–Trace Siriwardhana et al.(2006) p-Coumaric acid0.03b–––Amarowicz et al.(2005)Trace 3.09±0.01c– 1.34±0.01c Siriwardhana et al.(2006)–0.725c––Monagas et al.(2007) Chlorogenic acid–10.6–3.12c––Monagas et al.(2007)–––42.52±4.50a Takeoka and Dao(2003) Cryptochlorogenic acid–––7.90a Takeoka and Dao(2003) Neochlorogenic acid––– 3.04a Takeoka and Dao(2003)+Present.–Not found.a mg/100g of fresh weight.b mg catechin equivalents/g80%aqueous acetone extract.c l g/g.Table3Content of anthocyanidin and procyanidin in different parts of almond.Anthocyanidin and procyanidin Content ReferencesKernel Skin Shell HullDelphinidin0.05b–––Amarowicz et al.(2005)Cyanidin 1.76b–––Amarowicz et al.(2005)(+)-Catechin–90.1–36.4a––Monagas et al.(2007)–+––Sang,Chen,et al.(2002),Sang,Lapsley,et al.(2002),and Sang,Lapsley,Rosen,et al.(2002) (À)-Epicatechin–36.6–14.8a––Monagas et al.(2007)Procyanidin B3+B1–23.8–11.8a––Monagas et al.(2007)Procyanidin B2 1.24b–––Amarowicz et al.(2005)–16.1–5.34a––Monagas et al.(2007) Procyanidin B3 3.16b–––Amarowicz et al.(2005)Procyanidin B7–13.9–5.63a––Monagas et al.(2007)Procyanidin B5–8.57–3.46a––Monagas et al.(2007)Procyanidin C1–15.3–3.45a––Monagas et al.(2007)A-type procyanidin dimer(tr=31.3)– 6.98–3.18a––Monagas et al.(2007)A-type procyanidin dimer(tr=32.2)– 6.30–1.36a––Monagas et al.(2007)A-type procyanidin dimer(tr=35.7)–7.29–3.97a––Monagas et al.(2007)A-type procyanidin dimer(tr=48.9)– 2.04–0.70a––Monagas et al.(2007)A-type prodelphinidin dimer(tr=50.7)– 1.80–0.90a––Monagas et al.(2007)A-type procyanidin trimer(tr=30.8)– 4.28–1.58a––Monagas et al.(2007)+Present.–Not found.a l g/g.b mg catechin equivalents/g80%aqueous acetone extract.352 A.J.Esfahlan et al./Food Chemistry120(2010)349–360usually averaging about12%.In addition to these dry solubles,the hulls also contain insolublefibre,constructing of cellulose,hemi-cellulose,pectins,tannin-like complex polyphenols,and ash.As dry hulls,therefore,the almond mesocarp represent a potential source of useful foods,food additives,pharmaceuticals,and feed additives,over and above low value usage as roughage or cat litter (Rabinowitz,1991,2002,2004).In the past,almond hulls,a by-product of the almond industry,were removed from almonds after harvesting and used as supplemental livestock feed.Recently, there is interest in using almond hulls as a natural source for sweetener concentrate and dietaryfibre(Takeoka&Dao,2003).Al-mond hulls contain triterpenoids(Takeoka et al.,2000),lactones (Sang,Chen,et al.,2002),and phenolics(Sang,Lapsley,Rosen, et al.,2002).Takeoka and Dao(2003)extracted Almond hulls(Nonpareil variety)with methanol and analysed the extract,using reverse phase HPLC with diode array detection.The extract contained5-O-caffeoylquinic acid(chlorogenic acid),4-O-caffeoylquinic acid (cryptochlorogenic acid),and3-O-caffeoylquinic acid(neochloro-genic acid).The chlorogenic,cryptochlorogenic and neochlorogenicTable4Content offlavonol glycosides in different parts of almond.Flavonol glycosides Content ReferencesKernel Skin Shell HullKaempferol-3-O-rutinoside–12.8–31.8a––Monagas et al.(2007)–+––Frison and sporns(2002)++–+Wijeratne et al.(2006) Kaempferol-3-O-glucoside– 1.65a––Monagas et al.(2007)–+––Sang,Chen,et al.(2002),Sang,Lapsley,et al.(2002),and Sang,Lapsley,Rosen,et al.(2002)–+––Frison and sporns(2002) Isorhamnetin-3-O-rutinoside–27.6–41.4a––Monagas et al.(2007)–+––Frison and sporns(2002) Isorhamnetin-3-O-glucoside–15.6–8.85a––Monagas et al.(2007)–+––Frison and sporns(2002)++–+Wijeratne et al.(2006) Quercetin-3-O-glucoside– 2.41–1.33a––Monagas et al.(2007)–+––Sang,Chen,et al.(2002),Sang,Lapsley,et al.(2002),and Sang,Lapsley,Rosen,et al.(2002) Quercetin-3-O-galactoside–+––Sang,Chen,et al.(2002),Sang,Lapsley,et al.(2002),and Sang,Lapsley,Rosen,et al.(2002)+Present.–Not found.a l g/g.Table5Content offlavanone glycosides in different parts of almond.Flavanone glycosides Content ReferencesKernel Skin Shell HullNaringenin-7-O-glucoside–22.1–6.84a––Monagas et al.(2007)–+––Sang,Chen,et al.(2002),Sang,Lapsley,et al.(2002),and Sang,Lapsley,Rosen,et al.(2002) Eriodictyol-7-O-glucoside– 1.60–0.808a––Monagas et al.(2007)+Present.–Not found.a l g/g.Table6Content offlavonol aglycones in different parts of almond.Flavonol aglycones Content ReferencesKernel Skin Shell HullKaempferol0.17b–––Amarowicz et al.(2005)– 1.71–1.96a––Monagas et al.(2007) Quercetin0.14b–––Amarowicz et al.(2005)– 1.78–1.43a––Monagas et al.(2007)++–+Wijeratne et al.(2006) Quercitrin++–+Wijeratne et al.(2006) Isorhamnetin0.15b–––Amarowicz et al.(2005)– 4.87–4.19a––Monagas et al.(2007)++–+Wijeratne et al.(2006)+Present.–Not found.a l g/g.b mg catechin equivalents/g80%aqueous acetone extract.A.J.Esfahlan et al./Food Chemistry120(2010)349–360353acid concentration of almond hulls were42.52±4.50,7.90and 3.04mg/100g of fresh weight(n=4;moisture content=11.39%) or in the ratio79.5:14.8:5.7(Table2).Extracts were also tested for their ability to inhibit the oxidation of methyl linoleate at 40°C.At an equivalent concentration(10l g/1g of methyl linole-ate)almond hull extracts had higher antioxidant activity than a-tocopherol.At higher concentrations(50l g/1g of methyl linole-ate),almond hull extracts showed increased antioxidant activity that was similar to chlorogenic acid and morin[2-(2,4-dihydroxy-phenyl)-3,5,7-trihydroxy-4H-1-benzopyran-4-one]standards(at the same concentrations).These data indicate that almond hulls are a potential source of these dietary antioxidants.The sterols (3b,22E)-stigmasta-5,22-dien-3-ol(stigmasterol)and(3b)-stig-mast-5-en-3-ol(b-sitosterol)(Table8)identified by GC–MS of the silylated almond hull extract in this study.3.4.Almond shellAlmond shell is the name given to the ligneous material forming the thick endocarp or husk of the almond tree(P.amygdalus L.)fruit.When the fruit is processed to obtain the edible seeds,big lig-neous fragments are separated.These materials remain available as a waste product for which no important industrial use has been developed,so they are normally incinerated or dumped without control(Urrestarazu,Martinez,&Salas,2005).This industrial resi-due is the woody endocarp of the almond fruits.The processing by-products,shells and hulls,account for more than50%by dry weight of the almond fruits(Fadel,1999;Martinez et al.,1995). The high xylan content of almond shells makes them a suitable substrate for the production of xylose(Pou-Ilinas,Canellas, Driguez,Excoffier,&Vignon,1990),furfural(Quesada,Teffo-Bertaud,Croue,&Rubio,2002)or for fractionation into cellulose, pentosans,and lignin(Martinez et al.,1995).This latter utilisation consists of an acid-catalysed hydrolysis performed under mild con-ditions,which causes depolymerisation and solubilisation of the main components present in hemicelluloses.The liquid phase (hydrolysate)contains sugars,sugar-dehydration products,acetic acid and compounds derived from the acid-soluble lignin,which can be used for the production of oxyaromatics of interest for the health,cosmetics and food industries(Quesada et al.,2002).Al-mond shell is highly lignified(30–38%of the dry weight)(Martinez et al.,1995)and the guaiacyl to syringyl phenylpropane units ratio is similar to that of hardwoods(Quesada et al.,2002).Even if most of the lignin is acid-insoluble(Klason lignin),a part of it can be solubilised in acidic media.The antioxidant potential of depoly-merised lignin fractions produced after mild acid hydrolysis of lig-no-cellulosics has been reported(Cruz,Dominguez,&Parajo,2004, 2005;Garrote,Cruz,Dominguez,&Parajo,2003;Gonzalez,Cruz, Dominguez,&Parajo,2004).O-acetylated xylo-oligosaccharides (DXO)isolated from almond shells by autohydrolysis as well as their de-acetylated form(DeXO)were subjected to chemical, molecular,and structural analyses.They represent a mixture of neutral and acidic oligomers and low-molecular-weight polymers related to(4-O-methyl-D-glucurono)-D-xylan.DXO and DeXO showed direct mitogenic activity and enhancement of the T-mito-gen-induced proliferation of rat thymocytes,indicating the immu-nostimulatory potential of the almond shell xylo-oligosaccharides (Nabarlatz,Ebringerova,&Montane,2007).4.Potential application of almond by-products4.1.Almond shell as heavy metal adsorbentHeavy metals are nowadays amongst the most important pollu-tants in surface and ground waters.They are extremely toxic ele-ments,which can seriously affect plants and animals and have been cause a large number of afflictions(Taha,RIcordel,CIsse,& Dorange,2001).Therefore,elimination of these metals from water and wastewater is important in order to protect public health.For this reason,development of a new,flexible and environmentally friendly process for treatment of water and industrial effluents is a major challenge(Gaballah&KIlbertus,1998).The treatmentTable7Content of dihydroflavonol aglycones andflavanone aglycones phenolic compounds in different parts of almond.Dihydroflavonol aglyconesandflavanone aglyconesContent ReferencesKernel Skin Shell HullDihydroquercetin–Traces––Monagas et al.(2007) Naringenin– 2.83–4.01a––Monagas et al.(2007) Eriodictyol– 2.37–2.34a––Monagas et al.(2007) Morin++–+Wijeratne et al.(2006) +Present.–Not found.a l g/g.Table8Content of almond hulls sterols.Sterols Content ReferencesStigmasterol18.9a Takeoka and Dao(2003)b-Sitosterol16.0a Takeoka and Dao(2003)a mg/100g of almond hull.Table9Content of total phenolics in different parts of almond.Almond fruit parts Total phenolicscontentReferencesKernel16.1±0.4e Amarowicz et al.(2005)8.1±1.75a Siriwardhana and Shahidi(2002)8±1b Wijeratne et al.(2006)8±1b Siriwardhana et al.(2006)Skin87.8±1.75a Siriwardhana and Shahidi(2002)88±2b Wijeratne et al.(2006)88±2b Siriwardhana et al.(2006)413–242f Monagas et al.(2007)Shell 2.2c Moure et al.(2007)38.0±3.30d Jahanban et al.(2009)Hull71.1±1.74a Siriwardhana and Shahidi(2002)71±2b Wijeratne et al.(2006)71±2b Siriwardhana et al.(2006)78.2±3.41d Jahanban et al.(2009)a mg catechin equivalents/g ethanolic extract.b mg quercetin equivalents/g ethanolic extract.c g gallic acid equivalents/100g shells.d mg gallic acid equivalents/g methanolic extract.e mg catechin equivalents/g80%aqueous acetone extract.f l g/g.354 A.J.Esfahlan et al./Food Chemistry120(2010)349–360。
MBR反应器中SMP的研究吴鹏;沈耀良【摘要】Membrane bioreactor (MBR) process arouses more attention in recent years all over the world. However, membrane fouling is one of the main obstacles that restrict MBR to wide application while soluble microbial product (SMP) is an important factor of membrane fouling. The causes and fouling mechanisms of SMP are introduced,and the research on the causes of SMP and its influence on membrane fouling in China and abroad,as well as the SMP models,are summarized. At the end,the prevention measures of SMP membrane fouling are brought forward. It will be conducive to further application of MBR in the future.%膜生物反应器(MBR)在水处理领域的应用已引起人们的广泛关注.然而膜污染已成为制约MBR反应器广泛应用的主要障碍,其中溶解性微生物产物(SMP)又是影响膜污染的重要因素.为此,介绍了MBR反应器的膜污染成因与SMP膜污染机理,综述了近年来国内外关于SMP的成因及其对膜污染影响的研究进展,并对SMP的研究模型进行了总结,最后提出了SMP膜污染的防治措施,以利于MBR反应器的推广应用.【期刊名称】《工业水处理》【年(卷),期】2012(032)007【总页数】4页(P14-17)【关键词】膜生物反应器;膜污染;溶解性微生物产物【作者】吴鹏;沈耀良【作者单位】江南大学环境与土木工程学院,江苏无锡214122;苏州科技学院环境科学与工程省重点实验室,江苏苏州215011【正文语种】中文【中图分类】X703膜生物反应器(MBR)作为一种新型高效的生物处理技术和绿色技术,在水处理领域得到了广泛应用〔1〕,并已逐步应用于市政污水、工业废水的处理及回用中。
巨龙竹有机溶剂木质素提取及结构表征邓佳;史正军;成聃睿;杨海艳;令狐荣钢;郑志锋;刘蔚漪;辉朝茂【摘要】为表征巨龙竹木质素化学结构,在温和条件下连续采用二氧六环和二甲基亚砜抽提竹材原料,得到竹材木质素组分。
红外光谱、凝胶色谱和核磁共振分析结果表明,巨龙竹木质素属于典型的禾草类木质素,其大分子由对羟基苯丙烷( H)、愈创木基丙烷( G)和紫丁香基丙烷( S)三种基本结构单元组成;巨龙竹木质素大分子的主要联接键为β-O-4'醚键、β-β'和β-5'碳-碳键;在巨龙竹木质素大分子中,苯丙烷结构单元侧链γ位碳与对香豆酸存在化学键联接,形成对香豆酸酯。
%Bamboo lignin polymer was isolated from Dendrocalamus sinicus with dioxane and DMSO under mild conditions, and its'structural properties was characterized with Fourier transform infrared spectroscopy ( FTIR), gel permeation chromatography ( GPC ) , and nuclear magnetic resonance spectrum.The results indicated that the bamboo lignin belonged to grass lignin , consisting syringl ( S ) , guaiacyl ( G ) , and p-hydroxylphengl ( H ) units.The major interunit linkages presented in the obtained bamboo lignin were β-O-4'aryl ether linkages ,β-β'andβ-5'C-C linkages.In addition, a small percentage of the lignin side-chain was found to be esterfied by p-coumaric acid at th e γ-carbon.【期刊名称】《广州化工》【年(卷),期】2015(000)012【总页数】3页(P47-49)【关键词】巨龙竹;木质素;提取;结构表征;核磁共振【作者】邓佳;史正军;成聃睿;杨海艳;令狐荣钢;郑志锋;刘蔚漪;辉朝茂【作者单位】教育部省部共建西南山地森林资源保育与利用重点实验室,云南昆明650224;西南林业大学云南省高校生物质化学炼制与合成重点实验室,云南昆明650224;西南林业大学云南省高校生物质化学炼制与合成重点实验室,云南昆明650224;西南林业大学云南省高校生物质化学炼制与合成重点实验室,云南昆明650224;山东省临沂市林业局,山东临沂 276000;西南林业大学云南省高校生物质化学炼制与合成重点实验室,云南昆明 650224;教育部省部共建西南山地森林资源保育与利用重点实验室,云南昆明 650224;教育部省部共建西南山地森林资源保育与利用重点实验室,云南昆明 650224【正文语种】中文【中图分类】TQ654中国是世界竹类资源最丰富的国家,竹子种类和竹林面积居世界首位,具有发展竹产业的优越资源条件[1]。
基于B酸与L酸协同催化的乙酰丙酸酯化反应柳晨露;刘颖;武书彬【摘要】将甘蔗渣以碳化-磺化的方法制备出B酸催化剂(C-SO3 H),并与L酸CrCl3协同催化乙酰丙酸(LA)和乙醇的酯化反应,通过单因素实验和正交实验优化乙酰丙酸酯化反应,探究最佳反应条件.结果表明,在B酸与L酸质量比为1:1,反应摩尔比(LA:EtOH)为1:5,反应时间为8 h,反应温度为90℃的条件下,乙酰丙酸乙酯(ELA)的得率达89.7%.另外,对照组实验的结果说明,B酸与L酸在酯化反应中有一定的协同作用,提高了乙酰丙酸乙酯的得率.【期刊名称】《应用化工》【年(卷),期】2019(048)002【总页数】4页(P361-364)【关键词】甘蔗渣;B酸与L酸;乙酰丙酸乙酯;正交实验【作者】柳晨露;刘颖;武书彬【作者单位】华南理工大学制浆造纸与工程国家重点实验室,广东广州 510641;华南理工大学制浆造纸与工程国家重点实验室,广东广州 510641;华南理工大学制浆造纸与工程国家重点实验室,广东广州 510641【正文语种】中文【中图分类】TQ35随着能源消耗的剧增,全球不可再生能源资源日益枯竭,人类对可再生能源的开发和利用引起了越来越多的关注。
乙酰丙酸酯是一类非常有潜力的新能源化学品,具有广泛的工业应用价值[1-2]。
用于催化乙酰丙酸酯生成的催化剂主要包括无机酸[3-4]和固体酸[5-7]。
磺化碳催化剂因其显著的催化效果,可回收使用的优势而被广泛研究;其可通过纤维素[8-9]、木材[10-11]等碳化-磺化制备所得,应用于各类酸的酯化反应[12-13]。
各类无机盐催化剂,在酯化反应中表现突出,但不易回收。
为了探究B酸与L酸在酯化反应中的协同作用,本文通过单因素实验和正交实验获得乙酰丙酸(LA)与乙醇(EtOH)酯化生成乙酰丙酸乙酯(ELA)的最佳反应条件。
1 实验部分1.1 试剂与仪器蔗渣,取自蔗糖加工厂;乙酰丙酸、乙醇、甲苯、硫酸、氯化铬等均为分析纯。
Comparative studies on biomass production,life cycles and composting efficiency of Eisenia fetida (Savigny)and Lampito mauritii (Kinberg)G.Tripathi *,P.BhardwajDepartment of Zoology,JNV University,Jodhpur 342001,IndiaReceived 24October 2002;received in revised form 1September 2003;accepted 14September 2003AbstractComparative studies were performed to evaluate composting potential,biomass growth and biology of a non-native (Eisenia fetida )and an endemic (Lampito mauritii )species of earthworm in the semiarid environment of Jodhpur district of Rajasthan in India.Earthworms were reared in a mixed bedding material comprised of biogas slurry,cowdung,wheat straw,leaflitter,sawdust and kitchen waste.The percentage of organic carbon of the culture bedding material declined upto 105days with E.fetida and 120with L.mauritii .The percentage of nitrogen,phosphorous and potassium increased as a function of the vermicomposting period.In contrast,C/N and C/Pratios decreased day by day.Both species were effective for decomposition and mineralization of mixed bedding in the semiarid environment.A comparative assessment of biomass growth of E.fetida and L.mauritii was done under controlled laboratory conditions.The optimum temperature,moisture content and pH for E.fetida were 25°C,70%and 6.5,respectively.However,the optimum temperature,moisture content and pH for growth and development of L.mauritii were 30°C,60%and 7.5,respectively.The biology and reproductive rates of both species were also studied in the laboratory using mixed bedding.Cocoon production was higher for E.fetida than L.mauritii .The net reproductive rate was 9per month in the case of E.fetida and 1per month for L.mauritii .Fertilized eggs of E.fetida and L.mauritii developed into adults within 4and 51months,respectively.These observations indicate E.fetida may be a more efficient breeder than L.mauritii in the desert region of Rajasthan.Ó2003Elsevier Ltd.All rights reserved.Keywords:Eisenia fetida ;Lampito mauritii ;Vermicomposting;Biomass production;Biology1.IntroductionSoil animals are important contributors to soil fer-tility and humification processes (Singh and Pillai,1973).Further,earthworms should be considered key-stone organisms in regulating nutrient cycling processes in many ecosystems (Edwards,1988).Earthworms may alter the balance between ecosystem conservation and loss of nutrients,particularly carbon and nitrogen.Farmers consider them as beneficial because some spe-cies play a significant role in organic matter decompo-sition and mineral cycling (Edwards and Lofty,1977).Recycling of wastes through vermitechnology reduces the problem of nonutilization of agrowastes (Kale,2000).Vermicompost in recent years has gainedimportance because of its higher economic value com-pared with compost derived from traditional methods.Nutrients present in vermicompost are readily available for plant growth,and vermicompost has a higher con-centration of available nutrients than the waste from which it is formed (Buchanam et al.,1988).Jambhekar (1990)found that the application of vermicompost in-creased the available N,Pand K content in soil.The potential of earthworms as a biological tool should be much better understood to promote farming and sus-tainable development with the use of selected species of earthworms (Kale,1998).A great deal of work has been carried out on commercial earthworm farming in other countries,primarily for producing worms as bait for fishing but these worms could also be used profitably for recycling biodegradable wastes by vermicomposting techniques,not only as an alternate source of organic fertilizers but also for providing economical animal protein for fish and poultry feed.*Corresponding author.Address:D-41,Saraswati Nagar,New Pali Road,Jodhpur 342005,India.E-mail address:drgst@rediff (G.Tripathi).0960-8524/$-see front matter Ó2003Elsevier Ltd.All rights reserved.doi:10.1016/j.biortech.2003.09.005Bioresource Technology 92(2004)275–283For converting organic waste into valuable bioferti-lizers and protein biomass it is essential to know the influence of prevailing environmental factors on growth and reproduction of earthworms.The activities of earthworms are known to be influenced by quality of food(Lee,1985),moisture,density of worms and other abiotic factors(Biradar et al.,1999).Among these,food and environmental factors play an important role in the biology of earthworms.Hence to establish a ver-mifarm in a particular region,it is necessary to know the influence of waste-diets and the local environmental conditions on growth,reproduction and biology of earthworms.Energetic strategy and life table analysis of Lampito mauritii and Octochaetona surensis were stud-ied by Senapati and Dash(1991).They showed that L.mauritii was more resistant to environmental stress than O.surensis.Native species of earthworms are well-adapted to local conditions(Goswami and Kalita,2000).Hence choosing local or native species is afirst pre-requisite for launching a vermicomposting programme.But no information is available on composting potential and biology of any earthworm species inhabiting a desert environment,nor is there any report on adaptation of a non-native species to this harsh environment.Therefore, it was considered important to study the biology,life cycle and composting potential of a non-native(Eisenia fetida)and an endemic(L.mauritii)species in the desert region of Rajasthan.2.Methods2.1.Earthworm cultureTwo composting species of earthworms E.fetida (Savigny)and L.mauritii(Kinberg)were cultured in plastic pots(20cm height,28cm diameter)containing organic rich bedding.E.fetida was obtained from Prof. R.D.Kale,Department of Zoology,University of Agriculture Sciences,Bangalore and L.mauritii was collected from sewage sludge in Bhagat Ki Kothi, Jodhpur city of Rajasthan,by hand sorting method.The vermicomposting experiments were performed from October1999to February2000.The mixed substrate comprised biogas slurry,urine free cowdung,wheat straw,leaf litter,saw dust and kitchen waste used in equal amounts(w/w)as bedding material.Cultures were set-up in triplicate to analyse the effects of the earth-worms,while parallel control groups(without earth-worms)also were maintained.The culture pots were left for15days prior to experimentation and watering was done on alternate days for precomposting,microbial degradation,softening of wastes or thermostablization. After15days,50worms of similar age groups of each species(E.fetida and L.mauritii)were inoculated in the experimental plastic pots.The pH of bedding material was6.5–7.5.The moisture content was40–60%.The pH and moisture content of the bedding substrate was measured throughout the experiment.The pH of the substrate was maintained with the help of alkaline or acid water depending on requirement.The alkaline and acid waters were prepared using sodium hydroxide and hydrochloric acid,respectively.Similarly,moisture content was maintained with tap water.The culture pots were placed in a shady and moist place.Mulching was done using paddy straw to prevent evaporation of water. The organic carbon,total nitrogen,phosphorus and potassium were measured on a15day interval.C/N and C/Pratios were calculated from the measured values of C,N and P.The methods for nutrient analyses are given in the section on nutrient measurement.After150days of worm inoculation,total number of earthworms and cocoons were counted to evaluate the productivity of worm biomass in relation to physicochemical properties of bedding materials.Water logging was avoided to prevent anaerobiosis of the culture media.Care was taken not to injure the worms while handling,but,if any worm was injured it was replaced immediately by an-other worm of same size and weight in order to avoid infection.2.2.Biomass growth and biologyTo determine the effects of different temperatures, moisture contents and pHs on the biomass of juveniles, E.fetida and L.mauritii were cultured under controlled conditions in incubation chambers.The worms were cultured for90days(March–May2000)in mixed bed-ding at different temperatures(i.e.,15,20,25,30,and35°C).The moisture content and pH for culture during optimum temperature experiment was65–70%and6.5–7.4,respectively.Five one-week-old juveniles were wa-shed in tap water,blotted for adhering water,weighed and added to plastic culture pots of size4cm height and 16cm diameter.Three replicates of each culture pot were kept in incubators at selected temperatures.After 90days,the biomass of worms was recorded and bio-mass per worm was calculated.Afterwards an incubator was set at the observed optimum temperature and the effects of different moisture contents(i.e.,50±3%, 60±3%,70±3%and80±3%)on the biomass of worms were monitored to determine the optimum moisture at this temperature.After this,yet another experiment was conducted at the optimum temperature·moisture combination but pH was varied(6.5±0.1,7.5±0.1, 8.5±0.1and9.5±0.1).There were three replicate pots for the moisture and pH experiments each containing five juveniles of same age.Reproductive potential and biology of E.fetida and L.mauritii in mixed bedding material were also studied separately at their optimum conditions for6months.Six276G.Tripathi,P.Bhardwaj/Bioresource Technology92(2004)275–283adult worms were taken and cultured in plastic pots(20 cm height and28cm diameter)filled with mixed sub-strate as mentioned previously.The cocoons produced by these vermicomposting species within90days were counted and averaged as number of cocoons produced per worm per month.To determine the hatching success cocoons were collected and kept separately on moist cotton in separate petri dishes at25±2°C.Cocoons were observed daily.The incubation period until thefirst hatchling appeared was recorded.Subsequently,juve-niles were allowed to grow in mixed substrate for sub-sequent observation on their biology such as number of cocoons per month,number of hatchlings per cocoon and time to sexual maturity.The net reproductive rate of the worm per month was also calculated.The time required for juveniles to reach sexual maturity and for fertilized eggs to develop in adults also was observed.2.3.Analytical proceduresThe physicochemical properties of bedding material from each culture pot were also analysed:(a)Temperature:Soil thermometers were positioned at0.10m depth.(b)Moisture:Soil moisture content was estimated afteroven drying at105°C to a constant weight.(c)pH:Soil pH was determined using a digital hand pHmeter(ISO9001CE).Soil suspensions were pre-pared with soil and water(1:5w/v).(d)Nutrient measurement:organic carbon was deter-mined by the Walkley–Black method(1934).Total nitrogen was estimated with the help of a micro-kjeldhal procedure as described by Anderson and Ingram(1993).Available phosphorus was analysed by employing Olsen’s method(1954).Exchangeable potassium was determined in an atomic absorption spectrophotometer(Simard,1993).2.4.Statistical analysisOne-way ANOVA,regression and correlation coef-ficients were calculated according to the methods de-scribed by Croxton(1953).3.Results and discussion3.1.VermicompostingMixed bedding material with E.fetida and L.mauritii showed increases in N,Pand K,and decreases in C/N and C/Pratios,150days after worm inoculation(Figs.1 and2).ANOVA demonstrated a significant(P<0:05) change in these nutrients as a function of vermicompo-sting time.However,there was no significant variation in N,Pand K contents of the control with respect to composting time.The percentage of organic carbon declined up to105days for E.fetida and120days for L. mauritii in worms-worked bedding.Thereafter,it re-mained more or less constant up to150days.However, their control groups showed a gradual decrease in or-ganic carbon till150days.There were positive correla-tions between composting duration and N,Pand K shown both by E.fetida(r¼0:287,P<0:01;r¼0:689, P<0:001and r¼0:569,P<0:001,respectively)and L. mauritii(r¼0:326,P<0:001;r¼0:582,P<0:001and r¼0:510,P<0:001,respectively).However,organic carbon did not vary significantly with respect to com-posting time.The worm-worked bedding of E.fetida after150days exhibited1.19,3.32,1.61,1.13fold in-crease in organic carbon,N,Pand K as well as2.79and 1.35times decrease in C/N and C/Pratios,respectively as compared to their control values.In contrast,the worm-worked bedding of L.mauritii exhibited 1.13, 3.32,1.60and1.50fold increase in organic carbon,N,P and K but2.95and1.42times decrease in C/N and C/P ratios,respectively as compared to their control values. The differences in composting and vermicomposting led to the differences in decline of C/N ratio inspite of a similar decrease in percent carbon in control and vermi-composted chambers.For the control,the C/N ratio changes because carbon is lost,as CO2,due to aerobic fermentation and not because nitrogen is added.How-ever for vermicomposting,in addition to some losses in organic carbon there is also addition of nitrogen in the form of mucus,nitrogenous excretory substances, growth stimulating hormones and enzymes from earth-worms.These nitrogen rich biochemical substances were not already present in the bedding,hence they acted as additional amendment in worm-worked bedding to en-hance nitrogen level.This may be the reason why the C/ N ratio did not decline similarly in control and worm-worked beddings.Senapati et al.(1980)have also re-ported that the release of part of the carbon as CO2in the process of respiration,production of mucus and nitrogenous excrements enhances the level of nitrogen and lowers the C/N ratio in a closed vermicomposting system.There are also other reports of similar kind in vermicomposting related experiments(Karmegam and Daniel,2000;Bhadauria and Ramakrishnan,1996). After150days of vermicomposting,E.fetida produced an average of(40.7±1.2)cocoons and(167±1.5)total earthworms,whereas L.mauritii produced an average of (21.7±1.8)cocoons and(92.3±3.5)total worms.There was loss of organic carbon during thefirst3–4 months of composting in experimental vermibeds.This reduction in organic carbon could be achieved by respi-ratory activity of earthworms and microorganisms. Afterwards,it remained more or less constant or in-creased slightly.Whereas in control beddings organic carbon decreased slowly up to150days.After reduction,G.Tripathi,P.Bhardwaj/Bioresource Technology92(2004)275–283277the increase in organic carbon is due to the addition of their excreta in soil rich in organic carbon.These observations indicate that the process of composting is complete.Percentage of N,P and K increased gradually in vermibeds.I n contrast,C/N and C/Pratios declined as a function of increasing culture period.This suggests a faster decomposition with the passage of time.The presentfindings agree with the reports of Satchell (1983),Reinecke et al.(1992)and Singh(1997)who demonstrated similar changes in the chemical composi-tion of earthworm bedding materials as a result of casting activity.Vinceslas and Loquet(1997)have also described loss in organic carbon from45%to26%until the7th month of the experiment with E.fetida and afterwards it increased up to31%within a period of the next3months.Whereas in the control group organic carbon declined to25%till10th month,the decrease in C/N ratio was from62to30in the control group and from62to27in the vermicompost during a period of10 months.Dash and Senapati(1986)showed a decrease in the C/N ratio of earthworm cultures by31–61%.How-ever,Julka and Mukherjee(1986)showed an increase in carbon and N and decrease in the C/N ratio of culture soil after worm inoculation.The increase in carbon is contradictory to the present observations and it might be perhaps due to additional carbon from mucus and death of worms in culture medium.The earthworms catalyze the process of decomposition by reducing or fragmenting the organic residues and increasing the surface area for further decomposition by microflora. The release of carbon as carbon dioxide in the process of respiration and production of mucus and nitrogenous excrements enhance the level of N and lower the C/N ratio(Senapati et al.,1980).In the present study,thereduction in organic carbon,C/N and C/Pratios in vermicompost fall in line with the already reported re-sults(Edwards and Bohlen,1996;Ramalingam,1997; Karmegam and Daniel,2000).Chaudhuri et al.(2000) showed a decrease in C/N ratio from10.6to6.4and increase in Pfrom0.89%to1.09%after40days of composting by Perionyx excavatus in kitchen waste. Rise in the Pcontent during vermicomposting is prob-ably due to mineralization and mobilization of Pdue to bacterial and faecal phosphatase activity of earthworms (Krishnamoorthy,1990).A remarkable increase in N (32%),P(51%)and K(38%)content in P.excavatus worked vermicompost of sugarcane trash and cowdung substrate as compared to controls was reported by Ramalingam and Thilagar(2000).They further showed decrease in C/N(33%)and C/P(56%)ratios.The in-crease in N,Pand K in the vermicompost confirms the enhanced mineralization of these elements due to en-hanced microbial and enzyme activity in the guts of worms(Parthasarathi and Ranganathan,2000).3.2.Growth and biologyANOVA showed a significant(P<0:05)variation in biomass of E.fetida and L.mauritii as a function of increase in duration(days)at different temperatures. Similarly,biomass of these earthworms also varied sig-nificantly with changes in moisture and pH of vermibeds (Figs.3and4).Biomass of juveniles of E.fetida in-creased rapidly at25,30and35°C while at15and20°C,it increased slowly(Fig.3(A)).The average worm biomass at15°C after90days was0.337±0.137g/ worm.Similarly,at20,25and30°C the maximum biomass(g/worm at60th day)was0.585±0.069,0.863±0.257and 0.806±0.088,respectively.The bio-mass of juveniles of L.mauritii at 15°C,slowly in-creased for 75days and attained the maximum value of 0.313±0.024(Fig.4(A)).At 20°C,the worm biomass gradually increased for 75days and then declined.The maximum biomass (g/worm at 75th day)was 0.583±0.158.At 25,30and 35°C,worm biomass rapidly in-creased until 75days and remained more or less con-stant until day 90.The maximum biomass (g/worm)recorded was 0.905±0.079,0.993±0.063and 0.751±0.056at 25,30and 35°C,respectively after 75days of earthworm culturing.A temperature of 30°C produced the maximum growth of L.mauritii .The effects of different moisture contents (i.e.,50%,60%,70%and 80%)were studied at optimum growth temperatures,25°C for E.fetida and 30°C for L.mauritii (Figs.3(B)–4(B)).Maximum biomass of E.fetida (0.805±0.132g/worm)was observed at 70%moisture content,whereas minimum biomass (0.591±0.201g/worm)was obtained at 50%moisture content at 25°C.The maximum biomass (0.779±0.094g/worm)of L.mauritii was recorded at 60%moisture content,whereas minimum biomass (0.507±0.077g/worm)was observed at 50%moisture content.The effects of different pH (6.5,7.5,8.5and 9.5)on the biomass of E.fetida and L.mauritii cultured at their optimum temperature and moisture content were investigated.The biomass of E.fetida at 6.5pH was 0.793±0.063g/worm after 90days of rearing,and was less at higher pH (Fig.3(C)).The maximum biomass (0.795±0.095g/worm)of L.mauritii was recorded at 7.5pH (Fig.4(C)).Based on the data presented in Figs.3and 4,it is apparent that both E.fetida and L.mauritii cansurviveFig.3.Effects of different temperatures (A),moisture contents (B)and pH (C)on the biomass of juveniles of E.fetida under controlled conditions.Each point represents mean ±SEM of three observations after 90days of growth.280G.Tripathi,P.Bhardwaj /Bioresource Technology 92(2004)275–283at 15–35°C temperature.The optimum temperature for growth and development for E.fetida was 25°C.Graff(1953)also reported 25°C optimum temperature for biomass production of E.fetida .At this temperature,the optimum moisture content and pH for E.fetida were 70%and 6.5in this experiment.Edwards (1988)dem-onstrated that 20–30°C temperature,80–90%moisture and a pH of 5–9is optimal for growth and development of E.fetida .The present study showed that a tempera-ture of 30°C was optimal for the growth of L.mauritii ,similar to the optimum temperature requirement of P.excavatus (Reinecke and Hallat,1989).At 30°C,the optimum moisture content and pH for L.mauritii were 60%and 7.5,respectively.The difference in optimum requirements of E.fetida and L.mauritii may be due to their different origins,i.e.,temperate versus tropical,respectively.The cocoons produced by six individuals of both the species in mixed bedding material under controlled conditions after 90days were counted and are presented as number of cocoons produced per worm per month.The average cocoon production by E.fetida in mixed vermibed substrate was found to be 3.7±0.7cocoons per month.The cocoon of E.fetida took 53.7±3.5days to hatch and each cocoon on average produced three hatchlings within this hatching period.The percentage hatch was 81.9±2.6.The number of aclitellate worms emerged from each cocoon within a monthwasFig.4.Effects of different temperatures (A),moisture contents (B)and pH (C)on the biomass of juveniles of L.mauritii under controlled conditions.Each point represents mean ±SEM of three observations after 90days of growth.G.Tripathi,P.Bhardwaj /Bioresource Technology 92(2004)275–2832818.6±0.4,which reflects the net reproductive rate of the worm per month.Juveniles took67±3.8days to reach sexual maturity.The total time required for a fertilized egg to developed into an adult was124.3±4.1days.In contrast,the average cocoon production by L.mauritii in the same substrate was found to be1.7±0.7cocoons per month.The cocoon of L.mauritii took1.7±8.2days to hatch and each cocoon produced1.3±0.3hatchlings during the hatching period.The percentage hatch was 80.7±1.8.The net reproductive rate of the worm L. mauritii per month was1.2±0.2.Sexual maturity was obtained within89.3±4.7days.The total time required for a fertilized egg to develop into an adult was 157.3±3.3days.The reproduction rate of E.fetida was recorded as up to three cocoons per worm per week over a22week period by Hartenstein et al.(1979).However,Neuhauser et al.(1980)reported that E.fetida under controlled temperature(25°C)produced almost six cocoons per worm per week in mixed manure.This difference in production of cocoons may be due to nutritional status of the culture medium(Venter and Reinecke,1988). Hand(1988)found optimum temperature range for E. fetida between20and25°C.The onset of sexual maturity occurs between3and10weeks after hatching. The present observations on E.fetida may also be compared with the reports of Edwards(1988)who studied maximum number of hatchlings(3.3per cocoon) for E.fetida in animal and vegetable wastes as compared to Eudrilus euginea,P.excavatus and Dendrobaena veneta.Similar studies were done with L.mauritii reared on different vermibed substrates by Ponnuraj et al. (1998).From the present studies,it could be concluded that both E.fetida and L.mauritii are potential species for rearing and the mineralization of organic wastes in the Jodhpur district.Under controlled conditions the opti-mum temperature,moisture content and pH for growth and reproduction of E.fetida was25°C,70%and6.5, respectively,while for L.mauritii30°C temperature, 60%moisture content and7.5pH were optimum.The earthworm species E.fetida and L.mauritii completed their life cycle within4and51months,respectively in the desert region of Rajasthan.4.ConclusionExperiments were performed to determine the com-posting potential of a non-native(E.fetida)and a local species of Jodhpur(L.mauritii)over a period of150 days under laboratory conditions using mixed substrate as a bedding material.Our results obtained by vermi-composting of mixed substrate showed more minerali-zation by L.mauritii(3fold increase in nitrogen)than E. fetida(2.84fold increase in nitrogen).This concludes that L.mauritii may be used as a local composting species in the desert region of Rajasthan.Under con-trolled conditions the optimum temperature for growth and development for E.fetida was25°C.At this tem-perature,the optimum moisture content and pH for the same species were70%and6.5,respectively.Whereas for L.mauritii30°C,60%moisture content and7.5pH were optimum under same conditions.The difference in optimum requirements of both the species may be due to their different origins.Studies on biology of both the species showed that E.fetida(4months)completed their life cycle earlier than L.mauritii(514months)in the desert region of Rajasthan.The cocoon production was also higher in E.fetida(average four cocoons per month)than in L.mauritii(average two cocoons per month).Therefore,it could be concluded that E.fetida and L.mauritii are potential species for rearing and mineralization of organic waste in the Jodhpur district.AcknowledgementsThe authors express their gratitude to the Depart-ment of Biotechnology(DBT),Government of India, New Delhi for providingfinancial support.We are also thankful to Dr.G.Singh,AFRI,Jodhpur(India)for doing statistical analysis.ReferencesAnderson,J.M.,Ingram,J.S.I.,1993.Chemical analyses.In:Tropical Soil Biology and Fertility.A Handbook of Methods.CAB International Wallingford,UK,pp.71–73.Bhadauria,T.,Ramakrishnan,P.S.,1996.Earthworm population dynamics and contribution to nutrient cycling during cropping and follow phases of shifting agriculture(Jhum)in northeast India.J.Appl.Ecol.26,505–520.Biradar,V.R.,Amoji,S.D.,Shagoti,U.M.,Biradar,P.M.,1999.Seasonal variations in growth and reproduction of the earthworms Perionyx excavatus(Oligochaeta:Megascolecidae).Biol.Fertil.Soil.28,389–392.Buchanam,M.A.,Rusell,E.,Block,S.D.,1988.Chemical character-ization and nitrogen mineralization potentials of vermicomposte derived from differing organic wastes.In:Edwards,C.A.,Neuha-user, E.F.(Eds.),Earthworms in Environmental and Waste Management.SPB Academic Publishing,The Netherlands,pp.231–240.Chaudhuri,P.S.,Pal,T.K.,Bhattacharjee,G.,Dey,S.K.,2000.Chemical changes during vermicomposting(Perionyx excavatus)of kitchen waste.Trop.Ecol.41,107–110.Croxton,F.E.,1953.Linear correlation of two variables.In:Elemen-tary Statistics with Applications.Dover Publications Inc.,New York,pp.109–147.Dash,M.C.,Senapati,B.K.,1986.Vermitechnology,an option for organic waste management in India.In:Dash,M.C.,Senapati,B.K.,Mishra,P.C.,(Eds.),.Waste Utiliz.Vermicomp.Part B:Verms and Vermicomposting.Five Star Printing Press,Burla,India,pp.157–172.Edwards,C.A.,1988.Breakdown of animal,vegetable and industrial organic wastes by earthworm.In:Edwards,C.A.,Neuhauser,E.F.282G.Tripathi,P.Bhardwaj/Bioresource Technology92(2004)275–283(Eds.),Earthworms in Waste and Environmental Management.SPB,The Hague,pp.21–31.Edwards,C.A.,Bohlen,P.J.,1996.Biology and Ecology of Earth-worms,third ed.Chapman and Hall,London,England. 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