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使大鼠星形胶质细胞转化为神经元：谁做了有效的重编程？
在以往的报道中，需要2个及以上的转录因子才能有效地重编程体细胞为神经元。
成纤维细胞在转录因子Mash1和Brn2作用下即可转变成神经元。
而对于和神经元来自于同一胚层的胶质细胞，是否更容易转变成神经元？中国上海中医药大学王拥军领导的团队进行的一项研究，发现Brn2可在成年大鼠星形胶质细胞中内源性表达，因此推测仅仅Mash1基因即可实现胶质细胞向神经元的转变。
实验构建MSCV-MASH1重组质粒引入星形胶质细胞后1周，星形胶质细胞的形态发生明显变化，有典型神经元形态，且神经元标志基因β-tubullin表达水平显著升高。
说明Mash1单独作用于星形胶质细胞即可使其转变成神经元。
实验提供了一种快速有效获得神经元的方法，而且由于无细胞中间转化状态，避免了其体内实验时形成肿瘤的风险，可进一步为干细胞治疗提供基础依据。
此项研究成果发表在2014年1月第1期的《中国神经再生研究（英文版）》杂志上。
Mash1感染星形胶质细胞后1周，细胞阳性表达β-tubulin（绿色），蓝色为DAPI标记的细胞核
Article: " Mash1 efficiently reprograms rat astrocytes into neurons" by Daofang Ding1, 2, Leqin Xu1, 2, Hao Xu1, 2, Xiaofeng Li1, 2, Qianqian Liang1, 2, Yongjian Zhao1, 2, Yongjun Wang1, 2 (1 Institute of Spine, Shanghai University of Traditional Chinese Medicine, Shanghai, China; 2 Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China)
Ding DF, Xu LQ, Xu H, Li XF, Liang QQ, Zhao YJ, Wang YJ. Mash1 efficiently reprograms rat astrocytes into neurons . Neural Regen Res. 2014;9(1):25-32.
Who reprograms rat astrocytes into neurons?
To date, it remains poorly understood whether astrocytes can be easily reprogrammed into neurons. Mash1 and Brn2 have been previously shown to cooperate to reprogram fibroblasts into neurons. Dr. Yongjun Wang and team from Shanghai University of Traditional Chinese Medicine in China found that and found that Brn2 was expressed in astrocytes from
2-month-old Sprague-Dawley rats, but Mash1 was not detectable. Thus, the researchers hypothesized that Mash1 alone could be used to reprogram astrocytes into neurons.Murine stem cell virus (MSCV)-Mash1 recombinant plasmid was constructed and transfected into GP2-293t cells to produce retrovirus. One week later, the changes in morphology of astrocytes were detectable, which showed typical neuro nal characteristics. Moreover, β-tubulin expression levels were significantly higher in astrocytes expressing Mash1 than in control cells. These results, published in the Neural Regeneration Research (Vol. 9, No. 1, 2014), indicate that Mash1 alone can reprogram astrocytes into neurons. This method determined
an easier process of reprogramming through the endogenous expression of important transcription factors, which avoids malignant transformation due to the spontaneous reactivation of viral transgenes.
After 1 week, Mash1-transfected astrocytes were positive for β-tubulin (green) expression, and 4′,6-diamidino-2-phenylindole marked all cell nuclei in blue.
Article: " Mash1 efficiently reprograms rat astrocytes into neurons" by Daofang Ding1, 2, Leqin Xu1, 2, Hao Xu1, 2, Xiaofeng Li1, 2, Qianqian Liang1, 2, Yongjian Zhao1, 2, Yongjun Wang1, 2 (1 Institute of Spine, Shanghai University of Traditional Chinese Medicine, Shanghai, China; 2 Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China)
Ding DF, Xu LQ, Xu H, Li XF, Liang QQ, Zhao YJ, Wang YJ. Mash1 efficiently reprograms rat astrocytes into neurons . Neural Regen Res. 2014;9(1):25-32.
更多信息Neural Regeneration Research。
