D609_83373-60-8_CoA_MedChemExpress

D5319dat Rev 07/231Product InformationDeoxyribonuclease I bovineRecombinant, expressed in Pichia pastoris , buffered aqueous glycerol solution, ≥ 5,000 units/mg proteinD5319Product DescriptionCAS Registry Number: 9003-98-9Enzyme Commission (EC) Number: 3.1.21.1 Synonyms: DNase I,Deoxyribonucleate 5'-Oligonucleotidohydrolase Molecular mass: ~39 kDaExtinction Coefficient: E 2801% = 11.1Deoxyribonuclease I (DNase I) is an endonuclease that acts on phosphodiester bonds adjacent topyrimidines to produce polynucleotides with terminal 5'-phosphates. A tetranucleotide is the smallest average digestion product. DNase I hydrolyzes single-stranded and double-stranded DNA.•In the presence of Mg 2+ ions, DNase I attacks each strand of DNA independently and the cleavage sites are random.•If Mn 2+ ions are present, both DNA strands are cleaved at approximately the same site.1When chromatin DNA is digested, the reaction rate is restricted by the association of DNA with histones.1 DNase I is found in most cells and tissues. Inmammals, the pancreas is one of the best sources for the enzyme. Pancreatic DNase I was the first isolated DNase.DNase I can be used to remove DNA from protein and nucleic acid samples, and to nick DNA as a first step to incorporate labeled bases into DNA.This recombinant bovine DNase I is a glycoprotein, produced without the addition of any animal-derived materials. Several theses 2 and dissertations 3-7 have cited use of product D5319 in their protocols.ActivatorsDNase I has an absolute requirement for divalent metal cations:• Mg 2+ is the most commonly used divalent cation.8,9•Mn 2+, Ca 2+, Co 2+, and Zn 2+ will also activate DNase I.8-10A concentration of 5 mM Ca 2+ will stabilize DNase I against proteolytic digestion. 0.1 mM Ca 2+ is needed to reduce the rate of inactivation by one-half.11Inhibitors•2-Mercaptoethanol (the reduced enzyme is inactive, but can be reactivated in the presence of Ca 2+ or Mg 2+ ions)10 • Chelators (such as EDTA) • Sodium dodecyl sulfate (SDS)12 •Actin 13There is no single general inhibitor specific for DNase I.2 Citrate inhibits Mg 2+-activated DNase I, but not Mn 2+-activated DNase I.Optimal pHThe optimal pH of DNase I activity is dependent on the divalent ion present. In the presence of both Mg 2+ and Ca 2+, the optimal pH is between 7-8, while in the absence of Ca 2+, the optimal pH is between 5.5-6.0.14Precautions and DisclaimerThis product is for R&D use only. Not for drug,household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.The life science business of Merck operates as MilliporeSigma in the U.S. and Canada.Merck and Sigma-Aldrich are trademarks of Merck KGaA, Darmstadt, Germany or its affiliates. All other trademarks are the property of their respective owners. Detailed information on trademarks is available via publicly accessible resources.© 2015-2023 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved. D5319dat Rev 07/23 DT,GCY,LS,RBG,RC,MAM2ProductThis product is supplied as a solution in 4 mg/mL glycine (pH 5.0), 5 mM calcium acetate, and 50% glycerol.Specific activity: ≥ 5,000 units/mg proteinUnit definition: One unit will produce a change in A 260 of 0.001 per minute per mL at pH 5.0 at 25 °C using DNA, Type I or III, as the substrate. This enzyme assay reaction is performed in 83 mM acetate buffer (pH 5.0), at 25 °C, containing 4.2 mM Mg 2+, in a 3 mL reaction.ImpuritiesProtease: None Detected RNase: None detectedStorage/StabilityThis product retains activity for at least two years when stored at –20 °C.References1. Sambrook, J. et al ., Molecular Cloning: ALaboratory Manual , 2nd edition. Cold Spring Harbor Laboratory Press (Cold Spring Harbor, New York, USA), Vol. 2, p. 5.83 (1989). 2. Schöftner, Leonie Christine, "Development of ahuman 3D skin model to study cutaneous T cells". University of Salzburg, M.Sc. thesis, pp. 18, 21 (2021). 3. Alsiwiehri, Naif, "Identification andcharacterisation of novel cancer testis antigens in human cancer cells". Bangor University, Ph.D. dissertation, p. 35 (2017). 4. Deans, Jonathan Robert, "The Role of NuclearReceptors in Tissue-Specific Gene Expression: The Impact of Genetic Variation on DNA Binding". University of California Riverside, Ph.D. dissertation, p. 34 (2017). 5. Hempel, Heidi Anne, "The role of mast cells inprostate cancer initiation and recurrence". Johns Hopkins University, Ph.D. dissertation, p. 55 (2017). 6. Wu, Zhiyuan, "Immunophenotyping ofColorectal Cancer Reveals a Reduction of CD73 Expression on Tumor-Infiltrating Lymphocytes". Friedrich-Alexander-Universität Erlangen-Nürnberg, Dr. med. dissertation, p. 9 (2021).7. Hinterdobler, Julia Michaela, "Linkinginflammation and atherosclerosis – novel mechanistic insights into how vascular inflammation fuels progression ofatherosclerosis". Technischen UniversitätMünchen, Dr. rer. nat. dissertation, p. 31 (2022). 8. Weir, A. F., Methods Mol. Biol ., 16, 1-6 (1993). 9. Weir, A. F., Methods Mol. Biol ., 16, 7-16 (1993). 10. Price, P. A. et al ., J. Biol. Chem ., 244(3),929-932 (1969). 11. Price, P. A., et al ., J. Biol Chem ., 244(3),917-923 (1969). 12. Liao, T.-H., J. Biol. Chem ., 250(10), 3831-3836(1975). 13. Love, J. D., and Hewitt, R. R., J. Biol. Chem .,254(24), 12588-12594 (1979). 14. Lazarides, E., and Lindberg, U., Proc. Natl. Acad.Sci. USA , 71(12), 4742-4746 (1974).NoticeWe provide information and advice to our customers on application technologies and regulatory matters to the best of our knowledge and ability, but without obligation or liability. Existing laws and regulations are to be observed in all cases by our customers. This also applies in respect to any rights of third parties. Our information and advice do not relieve ourcustomers of their own responsibility for checking the suitability of our products for the envisaged purpose. The information in this document is subject to change without notice and should not be construed as acommitment by the manufacturing or selling entity, or an affiliate. We assume no responsibility for any errors that may appear in this document.Technical AssistanceVisit the tech service page at /techservice .Terms and Conditions of UseWarranty, use restrictions, and other conditions of sale may be found at /terms .Contact InformationFor the location of the office nearest you, go to /offices .。

25g
≥98%
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CAS
包装
纯度
MedBio
MED11116
Fmoc-(2-氯苄氧基羰基)赖氨酸
Fmoc-Lys(2-Cl-Z)-OH
133970-31-7
25g
≥98%
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CAS
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Fmoc-N-甲基-D-亮氨酸
Fmoc-D-N-Me-Leu-OH
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Boc-D-N-Me-Phe.DCHA
102185-45-5
25g
≥98%
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英文名称
CAS
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MedBio
MED11002
2-(叔-丁氧基碳酰胺)-2-苯乙腈
Boc-ON
58632-95-4
25g
≥98%
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货号
中文名称
英文名称
CAS
包装
纯度
MedBio
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N,N'-双芴甲氧羰基-L-赖氨酸
Fmoc-Lys(Fmoc)-OH
78081-87-5
100g
≥98%
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货号
中文名称
英文名称
CAS
包装
纯度
MedBio
MED11110
Fmoc-L-异亮氨酸
Fmoc-Ile-OH

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科研热词 固相萃取 高效液相色谱 萃取 药品和个人护理用品 综述 生物毒性 液相色谱-串联质谱 液相微萃取 检测方法 支撑液膜 抗生素 土壤 去除工艺 加速溶剂萃取 前处理技术 制药工业 高效液相荧光检测(hplc-fd) 高效液相色谱法 高效液相色谱-串联质谱 雌二醇 阿米替林 铜离子 酸奶 酶联免疫吸附检测 酚类化合物(aroh) 邻苯二甲酸二甲酯 选择性富集 迁移 超高效液相色谱/电喷雾/串联质谱 超高效液相色谱-串联质谱(uplc-ms/ms) 超高效液相色谱 超声波萃取 血药浓度 血清浓度 薄荷 葡萄汁 萃取富集 药物动力学 苯酚 芳烃化合物 色谱法 色谱分析 致嗅有机物 膜液流失 联用技术 羟基萘酚 硒 皂苷 白花蛇舌草 生物富集 环烯醚萜苷类 环境水样

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GMS10016.6 GMS10016.7 GMS10016.8 GMS10067.1 GMS10067.2 GMS10067.3 GMS10111.1 GMS10111.2 GMS10104.1 GMS10104.2
GMS10104.3
GMS10104.4
GMS10104.5
GMS10104.6
3． 移取
微升 GENMED 缓冲液（Reagent A）到新的 1 毫升比色杯
4． 加入
微升 GENMED 稀释液（Reagent C）
5． 放入分光光度仪，置零
6． 取出比色杯，加入
微升含有 GENMED 反应液（Reagent B）和 GENMED 稳定液（Reagent D）的 GENMED
11．上下倾倒数次，混匀
12．放入分光光度仪，置零
13．取出比色杯，加入
微升含有 GENMED 反应液（Reagent B）和 GENMED 稳定液（Reagent D）的 GENMED
反应工作液
14．即刻上下倾倒数次，混匀（限定在 3 秒之内）
15．即刻放入分光光度仪检测，此为样品读数（0 秒读数－60 秒读数），通常活性读数差值为正数
GENMED SCIENTIFICS INC. U.S.A
GMS10014.2 v.A
GENMED 纯化线粒体细胞色素 C 氧化酶活性测定试剂盒产品说明书（中文版）
主要用途
GENMED 纯化线粒体细胞色素 C 氧化酶活性测定试剂是一种旨在通过测定纯化的可溶性完整线粒体样品 中的细胞色素 C 氧化酶的活性，即采用比色法测定受到细胞色素 C 氧化酶催化的氧化反应的权威而经典的 技术方法。该技术由大师级科学家精心研制、成功实验证明的。其适用于各种线粒体（动物、人体、植物、 昆虫等）细胞色素 C 氧化酶的活性检测。产品严格无菌，即到即用，活体检测，操作简捷，性能稳定。