Put 2 ml of amylase inhibitor solution into tube 2
Keep the tube 3 as before
31
Step 3
Into each of three more boiling tubes put 2 ml amylase solution.
32
21
Step 6
Replace the tubes in the water bath at 370 C and measure the time taken for the blue/black colour to disappear.
22
Step 7
Test for sugar: add 1ml Benedict’s solution to each boiling tube and heat for 5 minutes.
Dr. A. K. M. Arif Uddin Ahmed.
Lecturer, Department of Pharmacology & Biochemistry.
Medical College of Xiamen University, China Next
28
Materials required
2
Prodedure Step 1
28°C
37°C
Set up five water baths at different temperatures, e.g. 0°C, 28°C, 37°C, 55°C, 80°C.
3
Step 2
Into each of five boiling tubes put 5 ml starch solution and 3 drops potassium iodide (I2-KI) solution.
