小麦HMW-GS与戊聚糖含量及其组分的相关分析和聚类分析

国外小麦品种资源HMW-GS组成分析薛晓强【摘要】This experiment detected and analyzed 11 foreign wheat cultivars resources of high molecular weight glutenin subunits (HMW-GS) composition,Glu-1 locus mutation and the frequency by conventional SDS-PAGE technology.The results showed that the Glu-A1 loci encoding HMW-GS had two types,2* and null.Glu-B1 loci encoding HMW-GS had four types,namely 7 + 9,6 + 8,7 + 18 and 6.8 + 20y,Glu-D1 loci encoding HMW-GS had 4 + 12,2 + 6.5,2 + 10,2 + 9,6.5 and 6 + 13 six types.The occurrence frequency of 2* subunit of Glu-A1 site,6 + 8 subunit of Glu-B1 site and 4 + 12 subunit of Glu-D1 site were higher.%以11份国外小麦品种资源为材料,利用聚丙烯酰胺凝胶电泳(SDS-PAGE)技术,对小麦材料高分子量谷蛋白亚基(HMW-GS)的组成、Glu-1位点变异类型及亚基出现的频率进行检测分析.结果表明,小麦品种的Glu-A1位点编码的HMW-GS有2种类型,即2*和Null;Glu-B1位点编码的HMW-GS有4种类型,即7+9,6+8,7+18和6.8+ 20y;Glu-D1位点编码的HMW-GS有6种类型,即4+12,2+6.5,2+10,2+9,6.5和6+13.其中,Glu-Al位点上的2*亚基、Glu-B1位点上的6+8亚基、Glu-D1位点上的4+12亚基出现频率最高.【期刊名称】《山西农业科学》【年(卷),期】2017(045)002【总页数】3页(P160-162)【关键词】小麦;HMW-GS;等位变异;Glu-1【作者】薛晓强【作者单位】山西运城农业职业技术学院,山西运城044000【正文语种】中文【中图分类】S512.1小麦籽粒中的麦谷蛋白和醇溶蛋白是形成面筋的主要成分，也是影响小麦加工品质的的主要因素[1-2]。

小麦-长穗偃麦草渗入系HMW-GS组成及等位变异分析王凌云;武宗信;贾举庆;张晓军;李欣;任文斌【摘要】长穗偃麦草(A gropyron elongatum)是小麦品质遗传改良的重要基因资源.为明确98份具有优异抗病性的小麦-长穗偃麦草渗入系中的高分子量麦谷蛋白亚基(HMW-GS)类型,利用SDS-PAGE法对其进行了调查.结果表明,共有13种等位基因变异,其中,Glu-A1位点上有1,2*和N型,N型亚基出现频率最高,为57.14％;Glu-B1位点上发现了6种等位变异,优质亚基14+ 15,7+8,13+ 16和17+ 18出现频率达到45.92％;Glu-D1位点上发现4种等位变异,出现频率最高的为2+ 12型,优质亚基5+10型达到20.41％.在22种亚基组合中,N,9+7,2+12组合出现的频率最高,为28.57％.品质为8～ 10分的材料有31份,频率达到31.5％.由此可见,在小麦-长穗偃麦草渗入系中存在丰富的高分子量谷蛋白亚基变异和优质组合,这为选育优质抗病的小麦品种提供了种质资源与依据.%Agropyron elongatum is an important resource for wheat genetic improvement.In order to clarify the types of high molecular weight glutenin subunits (HMW-GS) in 98 wheat-Agropyron elongatum introgression lines with excellent disease resistance,the method of SDS-PAGE was used.The results showed that there were 13 allelic variations among which there were 1,2 * and N types at Glu-A 1 locus and the highest frequency of N-type subunit was 57.14％.Six alleles were found at Glu-B1 locus and the frequencies of 14 + 15,7 + 8,13 + 16 and 17 + 18 with high quality subunits were45.92％.Four alleles were found at Glu-D1 locus with the highest frequency of 2 + 12 and high quality subunit 5 + 10 type reached 20.41％.Among the 22 subunit combinations,the combination of N,9 + 7 and 2 + 12 had thehighest frequency of 28.57％.There were 31 materials with a quality of 8-10 points and a frequency of 31.5％.Therefore,abundant high molecular weight glutenin subunit variation and high-quality combinations exist in these wheat-Agropyron elongatum introgression lines,which can provide the resources and basis for breeding high-quality and disease-resistant wheat varieties.【期刊名称】《山西农业科学》【年(卷),期】2018(046)006【总页数】5页(P867-870,879)【关键词】长穗偃麦草;渗入系;小麦;遗传改良;高分子量麦谷蛋白亚基【作者】王凌云;武宗信;贾举庆;张晓军;李欣;任文斌【作者单位】山西省农业科学院棉花研究所,山西运城044000;山西省农业科学院棉花研究所,山西运城044000;山西农业大学农学院,山西太谷030801;山西省农业科学院作物科学研究所,作物遗传与分子改良山西省重点实验室,山西太原030031;山西省农业科学院作物科学研究所,作物遗传与分子改良山西省重点实验室,山西太原030031;山西省农业科学院棉花研究所,山西运城044000【正文语种】中文【中图分类】S512高分子量麦谷蛋白亚基（HMW-GS）是小麦及其近缘物种种子中重要的贮藏蛋白，在面团黏弹性性能方面起着重要作用[1]。

黄淮麦区小麦新品系高分子量谷蛋白亚基的组成及结构变化分析李立;龚梦婕;李锁平;张大乐【摘要】为了解高分子量谷蛋白亚基(HMW-GS)组成对小麦品质产生的影响,利用SDS-PAGE技术对黄淮麦区221份小麦新品系的HMW-GS组成进行鉴定,对其系谱来源进行分析,并比较近10年来黄淮麦区小麦品种(系)的HMW-GS结构变化.结果表明,黄淮麦区小麦新品系的杂交亲本来源较为单一,具有周8425B和豫麦2号血统的材料高达155个,占到全部供试材料的70.1%,以郑麦9023等小偃系列和济麦20等鲁麦系列为亲本的材料分别有34,40个,占全部供试材料的15.4%和18.1%.221份供试材料中共出现13种亚基及亚基组合,其中在Glu-A1位点,亚基1出现频率最高,占供试材料的58.8%,自2008年以来,亚基1的出现频率高于Null 类型;在Glu-B1位点上,共出现7种亚基变异类型,出现频率较高的亚基组合是7+9和7+8,分别占供试材料的57.5%和30.3%,自2007年以来,7+9亚基组合类型的出现频率最高,优质强筋的17+18和13+16亚基组合的出现频率相对较低;在Glu-D1位点上,强筋亚基组合5+10占供试材料的15.4%,自2007年以来,5+10和2+12亚基组合的出现频率整体呈平行的趋势.本试验还检测出普通小麦品种中很少出现的5+12亚基组合,占供试材料的9.0%.%To understand the effect of high-molecular-weight glutenin subunit (HMW-GS) composition on wheat quality,the HMW-GS compositions of 221 new wheat lines from the Huang-Huai wheat area were character-ized through SDS-PAGE,and their pedigrees were analyzed. Additionally, the structural changes of HMW-GS in wheat varieties from the Huang-Huai wheat area during the last decade was investigated. Results showed that the cross parent of the new wheatlines from the study area was far less diversified. Up to 155 test materials descended from Zhoumai 8425B and Yumai 2,constituting 70.1% of the overall number. In comparison,34 and 40 test ma-terials descended from Zhengmai 9023 (and other Xiaoyan lines) and Jimai 20 (and other Lumai lines), respec-tively,constituting 15.4% and 18.1% of the total number. A total of 13 subunits and subunit compositions were i-dentified in the 221 materials tested. Among the Glu-A1 locus,the frequency of subunit 1 was the highest,consti-tuting 58.8%,and its occurrence frequency has exceeded that of subunit Null as of 2008. Seven subunits were found in the Glu-B1 locus,among which the frequencies of 7+9 and 7+8 subunit compositions were higher than the rest,constituting 57.5% and 30.3%,respectively. The occurrence frequency of 7+9 subunit was the highest at this locus as of 2007. However,the occurrence frequencies of subunit compositions relating to good gluten quali-ty were relatively low,such as 17+18 and13+16. Subunit composition 5+10 with strong gluten at Glu-D1 locus constituted 15.4% of the total number. The occurrence frequencies of5+10 and 2+12 subunit compositions ran paralleled as of 2007. The 5+12 subunit composition, which has rarely been found in historical wheat varieties, was also identified,constituting 9.0% of the total number.【期刊名称】《华北农学报》【年(卷),期】2018(033)002【总页数】6页(P157-162)【关键词】小麦;高分子量谷蛋白亚基;SDS-PAGE技术;黄淮麦区【作者】李立;龚梦婕;李锁平;张大乐【作者单位】中国科学院地理科学与资源研究所,北京 100012;河南大学生命科学学院,河南开封 475004;河南大学生命科学学院,河南开封 475004;河南大学生命科学学院,河南开封 475004【正文语种】中文【中图分类】S512.03小麦是世界上最重要的粮食作物之一，世界上有超过40%的人口以小麦为主食[1]，黄淮麦区是中国最大的小麦主产区，在育种家的不断努力下，小麦品种的产量潜力还在不断地提高。